                         SEQUENCE LISTING

<110>  Evonik Degussa GmbH
 
<120>  Verfahren zur fermentativen Herstellung von L-Ornithin

<130>  200900319

<160>  30    

<170>  PatentIn version 3.3

<210>  1
<211>  1901
<212>  DNA
<213>  Corynebacterium glutamicum ATCC13032


<220>
<221>  misc_feature
<222>  (930)..(61)
<223>  Komplementrer Strang der Kodierregion des Regulatorgens lysG 
       kodierend fr das Aktivatorprotein LysG

<220>
<221>  misc_feature
<222>  (1000)..(1000)
<223>  Transkriptionsstart des lysE-Gens

<220>
<221>  CDS
<222>  (1001)..(1702)
<223>  Kodierregion des lysE-Gens

<400>  1
cctggcccaa ttcctgcggg cgaagaagtg aaaaaccctg aaccttttca gaagtaacta     60

aggccgcaat ccctcgattg ctgcatcaac gacggcgtct gtgagtctag ctagagatct    120

agattccagg cgccatcgtt gccaatacat cggtgtgtca atgggtatct catcgaggag    180

gatcacttct cctgctttta gcatgggagc agcttgggtt tcgggaagaa gtccccaacc    240

aaggcctcgg cgaattgcct caccaaaacc ttccgccgac gggacaatgg atacgcgcct    300

gcgccccaca ggaccatcga cgcgcccgtc caggtcacgg tcttgaagca catctttggg    360

accgaagcgt aagacgggca tcgcagccca atctagtttc ccatcaacca tgtaggcatc    420

ccgcaatgag ggggttgcaa tggccaagtg gcgcatggtt ccaagttcta ctacttcaca    480

tcccgccacg ggattagctt cacgggttac cgctcctaaa acatctccac gccgcagcaa    540

ggataatgtg tgcgcttcat cttccaagcg cagcgtgagc gttgctccac cccaagaagc    600

tacctcgttg aacacgggag gaaaccatgt ggatagcgaa tctgcgttga tggcgatggt    660

taacgggatt tcagcaaggc gtccagatag ttgcgcttta gtttctgctt gcagcaacac    720

cattttccgc gctgcttgca caaggacttc acccgcttcg gttgctttgg ccggttgggt    780

gcgcgatacc aacactcgac ccacgtgatg ctcgagagct ttaacgcgct gactcaccgc    840

cgagggggaa atggaaaggg ctaaggaggc gccttcgaag ctgccttcat caatgattga    900

gagcaaagtg tccagttgaa tggggttcat gaagctatat taaaccatgt taagaaccaa    960

tcattttact taagtacttc cataggtcac gatggtgatc atg gaa atc ttc att     1015
                                            Met Glu Ile Phe Ile         
                                            1               5           

aca ggt ctg ctt ttg ggg gcc agt ctt tta ctg tcc atc gga ccg cag     1063
Thr Gly Leu Leu Leu Gly Ala Ser Leu Leu Leu Ser Ile Gly Pro Gln         
                10                  15                  20              

aat gta ctg gtg att aaa caa gga att aag cgc gaa gga ctc att gcg     1111
Asn Val Leu Val Ile Lys Gln Gly Ile Lys Arg Glu Gly Leu Ile Ala         
            25                  30                  35                  

gtt ctt ctc gtg tgt tta att tct gac gtc ttt ttg ttc atc gcc ggc     1159
Val Leu Leu Val Cys Leu Ile Ser Asp Val Phe Leu Phe Ile Ala Gly         
        40                  45                  50                      

acc ttg ggc gtt gat ctt ttg tcc aat gcc gcg ccg atc gtg ctc gat     1207
Thr Leu Gly Val Asp Leu Leu Ser Asn Ala Ala Pro Ile Val Leu Asp         
    55                  60                  65                          

att atg cgc tgg ggt ggc atc gct tac ctg tta tgg ttt gcc gtc atg     1255
Ile Met Arg Trp Gly Gly Ile Ala Tyr Leu Leu Trp Phe Ala Val Met         
70                  75                  80                  85          

gca gcg aaa gac gcc atg aca aac aag gtg gaa gcg cca cag atc att     1303
Ala Ala Lys Asp Ala Met Thr Asn Lys Val Glu Ala Pro Gln Ile Ile         
                90                  95                  100             

gaa gaa aca gaa cca acc gtg ccc gat gac acg cct ttg ggc ggt tcg     1351
Glu Glu Thr Glu Pro Thr Val Pro Asp Asp Thr Pro Leu Gly Gly Ser         
            105                 110                 115                 

gcg gtg gcc act gac acg cgc aac cgg gtg cgg gtg gag gtg agc gtc     1399
Ala Val Ala Thr Asp Thr Arg Asn Arg Val Arg Val Glu Val Ser Val         
        120                 125                 130                     

gat aag cag cgg gtt tgg gta aag ccc atg ttg atg gca atc gtg ctg     1447
Asp Lys Gln Arg Val Trp Val Lys Pro Met Leu Met Ala Ile Val Leu         
    135                 140                 145                         

acc tgg ttg aac ccg aat gcg tat ttg gac gcg ttt gtg ttt atc ggc     1495
Thr Trp Leu Asn Pro Asn Ala Tyr Leu Asp Ala Phe Val Phe Ile Gly         
150                 155                 160                 165         

ggc gtc ggc gcg caa tac ggc gac acc gga cgg tgg att ttc gcc gct     1543
Gly Val Gly Ala Gln Tyr Gly Asp Thr Gly Arg Trp Ile Phe Ala Ala         
                170                 175                 180             

ggc gcg ttc gcg gca agc ctg atc tgg ttc ccg ctg gtg ggt ttc ggc     1591
Gly Ala Phe Ala Ala Ser Leu Ile Trp Phe Pro Leu Val Gly Phe Gly         
            185                 190                 195                 

gca gca gca ttg tca cgc ccg ctg tcc agc ccc aag gtg tgg cgc tgg     1639
Ala Ala Ala Leu Ser Arg Pro Leu Ser Ser Pro Lys Val Trp Arg Trp         
        200                 205                 210                     

atc aac gtc gtc gtg gca gtt gtg atg acc gca ttg gcc atc aaa ctg     1687
Ile Asn Val Val Val Ala Val Val Met Thr Ala Leu Ala Ile Lys Leu         
    215                 220                 225                         

atg ttg atg ggt tag ttttcgcggg ttttggaatc ggtggccttc gcccaaatgt     1742
Met Leu Met Gly                                                         
230                                                                     

tgatgccggc gtcgtgggaa atctcatcga tcgcctccaa ctcggcgtca gaaaactcca   1802

agttgttgag tgaatcaagg ctgttgtcca gctgctcaac tgacgaagca ccaatcaatg   1862

cactggtcac ggtatccgcg ccgtactctc cttgctcgc                          1901


<210>  2
<211>  233
<212>  PRT
<213>  Corynebacterium glutamicum ATCC13032

<400>  2

Met Glu Ile Phe Ile Thr Gly Leu Leu Leu Gly Ala Ser Leu Leu Leu 
1               5                   10                  15      


Ser Ile Gly Pro Gln Asn Val Leu Val Ile Lys Gln Gly Ile Lys Arg 
            20                  25                  30          


Glu Gly Leu Ile Ala Val Leu Leu Val Cys Leu Ile Ser Asp Val Phe 
        35                  40                  45              


Leu Phe Ile Ala Gly Thr Leu Gly Val Asp Leu Leu Ser Asn Ala Ala 
    50                  55                  60                  


Pro Ile Val Leu Asp Ile Met Arg Trp Gly Gly Ile Ala Tyr Leu Leu 
65                  70                  75                  80  


Trp Phe Ala Val Met Ala Ala Lys Asp Ala Met Thr Asn Lys Val Glu 
                85                  90                  95      


Ala Pro Gln Ile Ile Glu Glu Thr Glu Pro Thr Val Pro Asp Asp Thr 
            100                 105                 110         


Pro Leu Gly Gly Ser Ala Val Ala Thr Asp Thr Arg Asn Arg Val Arg 
        115                 120                 125             


Val Glu Val Ser Val Asp Lys Gln Arg Val Trp Val Lys Pro Met Leu 
    130                 135                 140                 


Met Ala Ile Val Leu Thr Trp Leu Asn Pro Asn Ala Tyr Leu Asp Ala 
145                 150                 155                 160 


Phe Val Phe Ile Gly Gly Val Gly Ala Gln Tyr Gly Asp Thr Gly Arg 
                165                 170                 175     


Trp Ile Phe Ala Ala Gly Ala Phe Ala Ala Ser Leu Ile Trp Phe Pro 
            180                 185                 190         


Leu Val Gly Phe Gly Ala Ala Ala Leu Ser Arg Pro Leu Ser Ser Pro 
        195                 200                 205             


Lys Val Trp Arg Trp Ile Asn Val Val Val Ala Val Val Met Thr Ala 
    210                 215                 220                 


Leu Ala Ile Lys Leu Met Leu Met Gly 
225                 230             


<210>  3
<211>  761
<212>  DNA
<213>  Corynebacterium glutamicum ATCC13032


<220>
<221>  PCR-lysE-Fragment
<222>  (1)..(761)

<400>  3
tcgatatcat ggaaatcttc attacaggtc tgcttttggg ggccagtctt ttactgtcca     60

tcggaccgca gaatgtactg gtgattaaac aaggaattaa gcgcgaagga ctcattgcgg    120

ttcttctcgt gtgtttaatt tctgacgtct ttttgttcat cgccggcacc ttgggcgttg    180

atcttttgtc caatgccgcg ccgatcgtgc tcgatattat gcgctggggt ggcatcgctt    240

acctgttatg gtttgccgtc atggcagcga aagacgccat gacaaacaag gtggaagcgc    300

cacagatcat tgaagaaaca gaaccaaccg tgcccgatga cacgcctttg ggcggttcgg    360

cggtggccac tgacacgcgc aaccgggtgc gggtggaggt gagcgtcgat aagcagcggg    420

tttgggtaaa gcccatgttg atggcaatcg tgctgacctg gttgaacccg aatgcgtatt    480

tggacgcgtt tgtgtttatc ggcggcgtcg gcgcgcaata cggcgacacc ggacggtgga    540

ttttcgccgc tggcgcgttc gcggcaagcc tgatctggtt cccgctggtg ggtttcggcg    600

cagcagcatt gtcacgcccg ctgtccagcc ccaaggtgtg gcgctggatc aacgtcgtcg    660

tggcagttgt gatgaccgca ttggccatca aactgatgtt gatgggttag ttttcgcggg    720

ttttggaatc ggtggccttc gcccaaatat tgacctaggc a                        761


<210>  4
<211>  236
<212>  PRT
<213>  Corynebacterium glutamicum R


<220>
<221>  LysE
<222>  (1)..(236)

<400>  4

Met Val Ile Met Glu Ile Phe Ile Thr Gly Leu Leu Leu Gly Ala Ser 
1               5                   10                  15      


Leu Leu Leu Ser Ile Gly Pro Gln Asn Val Leu Val Ile Lys Gln Gly 
            20                  25                  30          


Ile Lys Arg Glu Gly Leu Ile Ala Val Leu Leu Val Cys Leu Ile Ser 
        35                  40                  45              


Asp Val Phe Leu Phe Ile Ala Gly Thr Leu Gly Val Asp Leu Leu Ser 
    50                  55                  60                  


Asn Ala Ala Pro Ile Val Leu Asp Ile Met Arg Trp Gly Gly Ile Ala 
65                  70                  75                  80  


Tyr Leu Leu Trp Phe Ala Val Met Ala Ala Lys Asp Ala Met Thr Asn 
                85                  90                  95      


Lys Val Glu Ala Pro Gln Ile Ile Glu Glu Thr Glu Pro Thr Val Pro 
            100                 105                 110         


Asp Asp Thr Pro Leu Gly Gly Ser Ala Val Ala Thr Asp Thr Arg Asn 
        115                 120                 125             


Arg Val Arg Val Glu Val Ser Val Asp Lys Gln Arg Val Trp Val Lys 
    130                 135                 140                 


Pro Met Leu Met Ala Ile Val Leu Thr Trp Leu Asn Pro Asn Ala Tyr 
145                 150                 155                 160 


Leu Asp Ala Phe Val Phe Ile Gly Gly Val Gly Ala Gln Tyr Gly Asp 
                165                 170                 175     


Thr Gly Arg Trp Ile Phe Ala Ala Gly Ala Phe Ala Ala Ser Leu Ile 
            180                 185                 190         


Trp Phe Pro Leu Val Gly Phe Gly Ala Ala Ala Leu Ser Arg Pro Leu 
        195                 200                 205             


Ser Ser Pro Lys Val Trp Arg Trp Ile Asn Val Val Val Ala Val Val 
    210                 215                 220                 


Met Thr Ala Leu Ala Ile Lys Leu Met Leu Met Gly 
225                 230                 235     


<210>  5
<211>  233
<212>  PRT
<213>  Corynebacterium glutamicum ATCC14067


<220>
<221>  LysE
<222>  (1)..(233)

<400>  5

Met Glu Ile Phe Ile Thr Gly Leu Leu Leu Gly Ala Ser Leu Leu Leu 
1               5                   10                  15      


Ser Ile Gly Pro Gln Asn Val Leu Val Ile Lys Gln Gly Ile Lys Arg 
            20                  25                  30          


Glu Gly Leu Ile Ala Val Leu Leu Val Cys Leu Ile Ser Asp Val Phe 
        35                  40                  45              


Leu Phe Ile Ala Gly Thr Leu Gly Val Asp Leu Leu Ser Asn Ala Ala 
    50                  55                  60                  


Pro Ile Val Leu Asp Ile Met Arg Trp Gly Gly Ile Ala Tyr Leu Leu 
65                  70                  75                  80  


Trp Phe Ala Val Met Ala Ala Lys Asp Ala Met Thr Asn Lys Val Glu 
                85                  90                  95      


Ala Pro Gln Ile Ile Glu Glu Thr Glu Pro Thr Val Pro Asp Asp Thr 
            100                 105                 110         


Pro Leu Gly Gly Ser Ala Val Ala Thr Asp Thr Arg Asn Arg Val Arg 
        115                 120                 125             


Val Glu Val Ser Val Asp Lys Gln Arg Val Trp Val Lys Pro Met Leu 
    130                 135                 140                 


Met Ala Ile Val Leu Thr Trp Leu Asn Pro Asn Ala Tyr Leu Asp Ala 
145                 150                 155                 160 


Phe Val Phe Ile Gly Gly Val Gly Ala Gln Tyr Gly Asp Thr Gly Arg 
                165                 170                 175     


Trp Ile Phe Ala Ala Gly Ala Phe Ala Ala Ser Leu Ile Trp Phe Pro 
            180                 185                 190         


Leu Val Gly Phe Gly Ala Ala Ala Leu Ser Arg Pro Leu Ser Ser Pro 
        195                 200                 205             


Lys Val Trp Arg Trp Ile Asn Val Val Val Ala Val Val Met Thr Ala 
    210                 215                 220                 


Leu Ala Ile Lys Leu Met Leu Met Gly 
225                 230             


<210>  6
<211>  702
<212>  DNA
<213>  Corynebacterium glutamicum ATCC14067


<220>
<221>  lysE-CDS
<222>  (1)..(702)

<400>  6
atggaaatct tcattacagg tctgcttttg ggggccagtc ttttgctgtc catcggaccg     60

cagaatgtac tggtgattaa acaaggaatt aagcgcgaag gactcattgc ggttcttctc    120

gtgtgtttaa tttctgacgt ctttttgttc atcgccggca ccttgggcgt tgatcttttg    180

tccaatgccg cgccgatcgt gctcgatatt atgcgctggg gtggtatcgc ttacctgtta    240

tggtttgccg tcatggcagc gaaagacgcc atgacaaaca aggtggaagc gccacagatc    300

attgaagaaa cagaaccaac cgtgcccgat gacacgcctt tgggcggttc ggcggtggcc    360

actgacacgc gcaaccgggt gcgggtggag gtgagcgtcg ataagcagcg ggtttgggta    420

aagcccatgt tgatggcaat cgtgctgacc tggttgaacc cgaatgcgta tttggacgcg    480

tttgtgttta tcggcggcgt cggcgcgcaa tacggcgaca ccggacggtg gattttcgcc    540

gctggcgcgt tcgcggcaag cctgatctgg ttcccgctgg tgggtttcgg cgcagcagca    600

ttgtcacgcc cgctgtccag ccccaaggtg tggcgctgga tcaacgtcgt cgtggcagtt    660

gtgatgaccg cattggccat caaactgatg ttgatgggtt ag                       702


<210>  7
<211>  233
<212>  PRT
<213>  Corynebacterium glutamicum ATCC13869


<220>
<221>  LysE
<222>  (1)..(233)

<400>  7

Met Glu Ile Phe Ile Thr Gly Leu Leu Leu Gly Ala Ser Leu Leu Leu 
1               5                   10                  15      


Ser Ile Gly Pro Gln Asn Val Leu Val Ile Lys Gln Gly Ile Lys Arg 
            20                  25                  30          


Glu Gly Leu Ile Ala Val Leu Leu Val Cys Leu Ile Ser Asp Val Phe 
        35                  40                  45              


Leu Phe Ile Ala Gly Thr Leu Gly Val Asp Leu Leu Ser Asn Ala Ala 
    50                  55                  60                  


Pro Ile Val Leu Asp Ile Met Arg Trp Gly Gly Ile Ala Tyr Leu Leu 
65                  70                  75                  80  


Trp Phe Ala Val Met Ala Ala Lys Asp Ala Met Thr Asn Lys Val Glu 
                85                  90                  95      


Ala Pro Gln Ile Ile Glu Glu Thr Glu Pro Thr Val Pro Asp Asp Thr 
            100                 105                 110         


Pro Leu Gly Gly Ser Ala Val Ala Thr Asp Thr Arg Asn Arg Val Arg 
        115                 120                 125             


Val Glu Val Ser Val Asp Lys Gln Arg Val Trp Val Lys Pro Met Leu 
    130                 135                 140                 


Met Ala Ile Val Leu Thr Trp Leu Asn Pro Asn Ala Tyr Leu Asp Ala 
145                 150                 155                 160 


Phe Val Phe Ile Gly Gly Val Gly Ala Gln Tyr Gly Asp Thr Gly Arg 
                165                 170                 175     


Trp Ile Phe Ala Ala Gly Ala Phe Ala Ala Ser Leu Ile Trp Phe Pro 
            180                 185                 190         


Leu Val Gly Phe Gly Ala Ala Ala Leu Ser Arg Pro Leu Ser Ser Pro 
        195                 200                 205             


Lys Val Trp Arg Trp Ile Asn Val Val Val Ala Val Val Met Thr Ala 
    210                 215                 220                 


Leu Ala Ile Lys Leu Met Leu Met Gly 
225                 230             


<210>  8
<211>  702
<212>  DNA
<213>  Corynebacterium glutamicum ATCC13869


<220>
<221>  lysE-CDS
<222>  (1)..(702)

<400>  8
atggaaatct tcattacagg tctgcttttg ggggccagtc ttttgctgtc catcggaccg     60

cagaatgtac tggtgattaa acaaggaatt aagcgcgaag gactcattgc ggttcttctc    120

gtgtgtttaa tttctgacgt ctttttgttc atcgccggca ccttgggcgt tgatcttttg    180

tccaatgccg cgccgatcgt gctcgatatt atgcgctggg gtggcatcgc ttacctgtta    240

tggtttgccg tcatggcagc gaaagacgcc atgacaaaca aggtggaagc gccacagatc    300

attgaagaaa cagaaccaac cgtgcccgat gacacgcctt tgggcggttc ggcggtggcc    360

actgacacgc gcaaccgggt gcgggtggag gtgagcgtcg ataagcagcg ggtttgggtg    420

aagcccatgt tgatggcaat cgtgctgacc tggttgaacc cgaatgcgta tttggacgcg    480

tttgtgttta tcggcggcgt cggcgcgcaa tacggcgaca ccggacggtg gattttcgcc    540

gctggcgcgt tcgcggcaag cctgatctgg ttcccgctgg tgggtttcgg cgcagcagca    600

ttgtcacgcc cgctgtccag ccccaaggtg tggcgctgga tcaacgtcgt cgtggcagtt    660

gtgatgaccg cattggccat caaactgatg ttgatgggtt ag                       702


<210>  9
<211>  228
<212>  PRT
<213>  Corynebacterium efficiens YS-314


<220>
<221>  LysE
<222>  (1)..(228)

<400>  9

Met Glu Ile Phe Val Thr Gly Leu Leu Leu Gly Ala Ser Leu Leu Leu 
1               5                   10                  15      


Ala Ile Gly Pro Gln Asn Val Leu Val Ile Lys Gln Gly Ile Lys Arg 
            20                  25                  30          


Glu Gly Ile Thr Ala Val Ile Ile Val Cys Leu Leu Ser Asp Val Val 
        35                  40                  45              


Leu Phe Thr Leu Gly Thr Leu Gly Val Gly Leu Ile Ser Asp Thr Ala 
    50                  55                  60                  


Pro Ile Ile Leu Asp Ile Leu Arg Trp Cys Gly Ile Ala Tyr Leu Leu 
65                  70                  75                  80  


Trp Phe Ala Val Met Ala Ala Arg Asp Ala Leu Arg Ala Arg Thr Glu 
                85                  90                  95      


Val Thr Phe Val Glu His Ser Glu Pro Val Ala Ala Ala Ser Ala Ser 
            100                 105                 110         


Gly Gly Gly Val Thr Thr Lys Gln Arg Pro Arg Leu Arg Ile Thr Ser 
        115                 120                 125             


Gly Thr Arg Gln Val Trp Val Arg Pro Met Leu Met Ala Ile Val Leu 
    130                 135                 140                 


Thr Trp Leu Asn Pro Asn Ala Tyr Leu Asp Ala Phe Val Phe Ile Gly 
145                 150                 155                 160 


Gly Val Gly Ala Gln Tyr Gly Glu Thr Gly Arg Trp Ile Phe Ala Ala 
                165                 170                 175     


Gly Ala Phe Ala Ala Ser Leu Val Trp Phe Pro Leu Val Gly Tyr Gly 
            180                 185                 190         


Ala Ala Ala Leu Ser Arg Pro Leu Ser Ser Pro Arg Val Trp Arg Trp 
        195                 200                 205             


Ile Asn Ile Gly Val Ala Val Val Leu Thr Gly Leu Ala Val Lys Leu 
    210                 215                 220                 


Ile Leu Met Gly 
225             


<210>  10
<211>  228
<212>  PRT
<213>  Corynebacterium diphteriae NCTC13129


<220>
<221>  LysE
<222>  (1)..(228)

<400>  10

Met Ser Ile Ala Ile Ala Gly Phe Leu Met Gly Leu Ser Leu Ile Val 
1               5                   10                  15      


Ala Ile Gly Pro Gln Asn Ala Leu Ile Ile Arg Gln Gly Ile Lys Arg 
            20                  25                  30          


Glu Gly Leu Ile Pro Ile Leu Val Val Cys Ile Leu Ser Asp Val Ile 
        35                  40                  45              


Leu Ile Phe Gly Gly Thr Ala Gly Val Gly Ala Leu Val Asp Arg Ala 
    50                  55                  60                  


Pro Ile Ala Leu Val Val Leu Lys Trp Leu Gly Val Ala Tyr Leu Leu 
65                  70                  75                  80  


Tyr Phe Gly Phe Thr Cys Phe Lys Glu Ala Phe Lys Arg His Gly Gln 
                85                  90                  95      


Ala Leu Ala Val Glu Gln Ser Glu Pro Val Ala Tyr Glu Pro Val Ala 
            100                 105                 110         


Asp Ala Ser Ser Gly Val Ile Thr Lys Thr Arg Thr Lys Ala Gln Pro 
        115                 120                 125             


Lys Ser Ala Gln Arg Thr Trp Val Lys Pro Val Leu Ala Ala Leu Ala 
    130                 135                 140                 


Phe Thr Trp Leu Asn Pro Ala Ala Tyr Ile Asp Val Leu Val Met Leu 
145                 150                 155                 160 


Gly Gly Ile Ala Asn Gln His Gly Pro Asp Gly Arg Trp Val Phe Ala 
                165                 170                 175     


Leu Gly Ala Leu Cys Ala Ser Leu Thr Trp Phe Pro Phe Ile Gly Tyr 
            180                 185                 190         


Thr Ser Thr Arg Phe Ser Thr Val Leu Ser Arg Pro Ala Val Trp Arg 
        195                 200                 205             


Tyr Ile Asn Ile Ala Ile Gly Ile Ile Met Met Ile Met Cys Ala Arg 
    210                 215                 220                 


Leu Ile Met His 
225             


<210>  11
<211>  222
<212>  PRT
<213>  Corynebacterium striatum ATCC6940


<220>
<221>  LysE
<222>  (1)..(222)

<400>  11

Met Ser Val Leu Leu Ala Gly Phe Leu Leu Gly Leu Ser Leu Ile Val 
1               5                   10                  15      


Ala Ile Gly Pro Gln Asn Ala Tyr Ile Ile Lys Met Gly Val Lys Arg 
            20                  25                  30          


Asp His Ile Gly Ala Ile Ile Leu Ala Cys Leu Leu Ser Asp Val Ile 
        35                  40                  45              


Leu Ile Asn Ala Gly Val Gly Gly Met Gly Val Leu Val Glu Lys Phe 
    50                  55                  60                  


Pro Thr Gly Leu Ile Ile Met Lys Tyr Leu Gly Ala Ala Tyr Leu Ile 
65                  70                  75                  80  


Tyr Phe Gly Phe Thr Cys Phe Arg Asp Ala Phe Lys Lys Glu Gln Glu 
                85                  90                  95      


Ala Leu Val Val Ser Ser Thr Pro Pro Ser Ala Pro Asn Glu Thr Glu 
            100                 105                 110         


Leu Gly Gly Ala Thr Thr Val Met Thr Lys Gln Arg Thr Lys Ser Arg 
        115                 120                 125             


Thr Trp Val Lys Pro Val Met Gly Ala Met Ala Leu Thr Trp Leu Asn 
    130                 135                 140                 


Pro Leu Ala Tyr Val Asp Val Leu Val Met Leu Gly Gly Ile Ala Gln 
145                 150                 155                 160 


His Tyr Gly Asp Gln Arg Trp Val Phe Ala Ala Gly Ala Ile Met Ala 
                165                 170                 175     


Ser Ala Val Trp Phe Pro Thr Val Gly Tyr Gly Ala Phe Lys Leu Ser 
            180                 185                 190         


His Val Leu Ala Lys Pro Thr Thr Trp Arg Tyr Val Asn Phe Ala Ile 
        195                 200                 205             


Gly Cys Val Met Met Leu Leu Thr Ala Lys Leu Leu Leu His 
    210                 215                 220         


<210>  12
<211>  235
<212>  PRT
<213>  Corynebacterium aurimucosum ATCC700975


<220>
<221>  LysE
<222>  (1)..(235)

<400>  12

Met Arg Arg Leu Glu Ala Met Ser Val Leu Leu Ala Gly Phe Ala Leu 
1               5                   10                  15      


Gly Leu Ser Leu Ile Ile Ala Ile Gly Pro Gln Asn Ala Tyr Ile Ile 
            20                  25                  30          


Lys Met Gly Ile Lys Arg Asp His Val Gly Pro Ile Leu Leu Ala Cys 
        35                  40                  45              


Leu Leu Ser Asp Val Ile Leu Ile Thr Gly Gly Thr Ala Gly Val Gly 
    50                  55                  60                  


Val Leu Val Glu Arg Phe Pro Thr Ala Leu Val Val Val Lys Tyr Leu 
65                  70                  75                  80  


Gly Ala Ala Tyr Leu Ile Tyr Phe Gly Phe Thr Cys Phe Arg Asp Ala 
                85                  90                  95      


Phe Lys Lys Gln Gln Asp Ala Leu Val Ile Glu Glu Thr Thr Pro Val 
            100                 105                 110         


Ala Gln Val Val Asp Glu Asn Ser Gly Asn Ala Gly Ala Pro Gly Thr 
        115                 120                 125             


Ser Val Leu Thr Lys Ile Arg Pro Arg Val Arg Ser Lys Ser Trp Val 
    130                 135                 140                 


Lys Pro Val Leu Gly Ala Leu Ala Leu Thr Trp Leu Asn Pro Leu Ala 
145                 150                 155                 160 


Tyr Val Asp Ala Leu Val Met Leu Gly Ser Ile Ala Asn Gln Tyr Gly 
                165                 170                 175     


Asp Gln Arg Trp Val Phe Ala Gly Gly Ala Ile Leu Ala Ser Ala Val 
            180                 185                 190         


Trp Phe Pro Ser Leu Gly Phe Gly Ala Tyr Lys Leu Ser His Val Leu 
        195                 200                 205             


Ala Lys Pro Thr Thr Trp Arg Val Val Asn Ile Val Ile Gly Cys Val 
    210                 215                 220                 


Met Leu Ala Leu Thr Ala Lys Leu Leu Phe Leu 
225                 230                 235 


<210>  13
<211>  244
<212>  PRT
<213>  Corynebacterium matruchotii ATCC33806


<220>
<221>  LysE
<222>  (1)..(244)

<400>  13

Met Ser Ile Ala Val Ala Gly Phe Leu Leu Gly Leu Ser Leu Ile Val 
1               5                   10                  15      


Ala Ile Gly Pro Gln Asn Ala Leu Val Ile Arg Gln Gly Val Lys Arg 
            20                  25                  30          


Glu Gly Leu Ile Val Val Leu Ala Ile Cys Ile Leu Ser Asp Ile Phe 
        35                  40                  45              


Leu Ile Phe Gly Gly Thr Ala Gly Val Gly Val Ile Ile Glu Lys Ala 
    50                  55                  60                  


Pro Leu Ala Leu Val Ala Leu Lys Trp Phe Gly Ala Ala Tyr Leu Ala 
65                  70                  75                  80  


Trp Phe Ala Val Ser Cys Phe Arg Asp Met Val Lys Pro Arg Ala Leu 
                85                  90                  95      


Asp Ser Ser Ala Thr Asp Asp Gly Thr Ser Leu Asp Asp Ala Pro Thr 
            100                 105                 110         


Ala Ala His Val Ser Asn Val Asp Thr Thr Ser Gly Asn Gly Gly Gln 
        115                 120                 125             


Val Gln Thr Lys Thr Arg Pro Ile Thr Thr Thr Ala Pro Thr Arg Gln 
    130                 135                 140                 


Ala His Pro Ala Arg Pro Trp Val Lys Pro Ala Leu Ala Ala Leu Ala 
145                 150                 155                 160 


Phe Thr Trp Leu Asn Pro Ser Ala Tyr Ile Asp Thr Leu Val Met Leu 
                165                 170                 175     


Gly Gly Ile Ala Asn Gln His Gly Glu Ser Gly Arg Trp Val Phe Ala 
            180                 185                 190         


Ala Gly Ala Leu Met Ala Ser Ala Val Trp Phe Pro Leu Leu Gly Phe 
        195                 200                 205             


Phe Ser Thr Arg Phe Ser Arg Val Leu Ser Arg Pro Gln Ala Trp Arg 
    210                 215                 220                 


Val Ile Asn Gly Val Ile Gly Cys Ile Met Val Val Met Cys Ile Arg 
225                 230                 235                 240 


Leu Val Met His 
                


<210>  14
<211>  230
<212>  PRT
<213>  Corynebacterium pseudogenitalium ATCC33035


<220>
<221>  LysE
<222>  (1)..(230)

<400>  14

Met Ser Ile Val Leu Ala Gly Phe Phe Leu Gly Leu Ser Leu Ile Val 
1               5                   10                  15      


Ala Val Gly Pro Gln Asn Ala Met Leu Leu Lys Tyr Gly Ile Arg Arg 
            20                  25                  30          


Asp His Ile Gly Leu Ile Ile Val Val Cys Ala Leu Ser Asp Val Ile 
        35                  40                  45              


Leu Ile Thr Ser Gly Thr Ala Gly Val Gly Tyr Leu Val Glu Lys Phe 
    50                  55                  60                  


Pro Asn Ala Leu Gln Val Leu Lys Tyr Val Gly Ala Ala Tyr Leu Ala 
65                  70                  75                  80  


Tyr Phe Thr Phe Thr Cys Phe Arg Asp Ala Phe Lys Thr Lys Gly Glu 
                85                  90                  95      


Ala Ile Glu Val Glu Ser Thr Gln Pro Lys Ala Pro Gln Glu Val Ala 
            100                 105                 110         


Ser Phe Asp Gly Ser Gln Ala Arg Ser Thr Thr Lys Thr Ala Ala Arg 
        115                 120                 125             


Val Glu Ile Lys Arg Ser Pro Ser Trp Val Lys Pro Leu Leu Thr Ala 
    130                 135                 140                 


Leu Ala Leu Thr Trp Leu Asn Pro Gly Ala Tyr Val Asp Val Val Val 
145                 150                 155                 160 


Met Leu Gly Ser Ile Ala Asn Gln Tyr Gly Glu Ser Gly Arg Trp Leu 
                165                 170                 175     


Phe Ala Val Gly Ala Ile Cys Ala Ser Phe Thr Trp Phe Pro Phe Ile 
            180                 185                 190         


Gly Phe Gly Ala Ala Arg Phe Ser His Val Leu Ser Arg Pro Thr Val 
        195                 200                 205             


Trp Arg Trp Ile Asn Phe Gly Ile Gly Val Ile Met Ile Gly Leu Thr 
    210                 215                 220                 


Leu Lys Leu Leu Leu Leu 
225                 230 


<210>  15
<211>  241
<212>  PRT
<213>  Corynebacterium accolens ATCC49725


<220>
<221>  LysE
<222>  (1)..(241)

<400>  15

Met Ser Ile Val Leu Ala Gly Phe Val Leu Gly Leu Ser Leu Ile Val 
1               5                   10                  15      


Ala Val Gly Pro Gln Asn Ala Met Leu Leu Lys Tyr Gly Ile Arg Arg 
            20                  25                  30          


Asp His Ile Gly Leu Ile Ile Val Val Cys Ala Leu Ser Asp Val Ile 
        35                  40                  45              


Leu Ile Thr Ser Gly Thr Ala Gly Val Gly Tyr Leu Val Glu Arg Phe 
    50                  55                  60                  


Pro Asn Ala Leu Glu Ala Leu Lys Tyr Ile Gly Ala Ala Tyr Leu Ala 
65                  70                  75                  80  


Phe Phe Thr Phe Thr Cys Phe Arg Asp Ala Phe Lys Thr Lys Gly Glu 
                85                  90                  95      


Ala Ile Asp Val Glu Ser Thr Ser Pro Asn Ser Thr Glu Glu Val Ala 
            100                 105                 110         


Thr Phe Asp Gly Asp Gly Asp Ser Thr Gly Gly Val Gly Thr Glu His 
        115                 120                 125             


Gly Ser Val Ala Thr Ala Thr Ala Thr Gln Arg Gln Glu Ile Lys Arg 
    130                 135                 140                 


Ser Pro Ser Trp Val Lys Pro Leu Leu Thr Ala Leu Ala Leu Thr Trp 
145                 150                 155                 160 


Leu Asn Pro Gly Ala Tyr Val Asp Val Leu Val Met Leu Gly Gly Ile 
                165                 170                 175     


Ala Asn Gln Tyr Gly Asp Pro Gly Arg Trp Leu Phe Ala Gly Gly Ala 
            180                 185                 190         


Ile Ala Ala Ser Phe Thr Trp Phe Pro Val Ile Gly Phe Gly Ala Ala 
        195                 200                 205             


Arg Phe Ser His Val Leu Ser Arg Pro Glu Val Trp Arg Trp Ile Asn 
    210                 215                 220                 


Val Gly Ile Gly Val Ile Met Ile Gly Leu Thr Leu Lys Leu Leu Leu 
225                 230                 235                 240 


Leu 
    


<210>  16
<211>  261
<212>  PRT
<213>  Corynebacterium glucuronalyticum ATCC51867

<400>  16

Met Val Pro Glu Asn Leu Ser Phe Tyr Leu Cys Val Leu Leu Thr His 
1               5                   10                  15      


Asn Lys Tyr Val Asn Val Phe Phe Ala Gly Leu Leu Phe Asn Leu Ser 
            20                  25                  30          


Leu Ile Leu Ala Leu Gly Pro Gln Asn Ala Leu Ile Leu Lys Tyr Gly 
        35                  40                  45              


Leu Arg Arg Gln Ala Ile Thr Leu Val Ile Ser Val Cys Ala Leu Cys 
    50                  55                  60                  


Asp Ile Thr Leu Ile Ala Leu Ser Gly Val Gly Val Gly Val Ile Leu 
65                  70                  75                  80  


Gln Lys Ala Pro Ile Val Leu Glu Ile Leu Arg Tyr Ala Gly Phe Leu 
                85                  90                  95      


Tyr Leu Leu Trp Phe Ala Tyr Thr Cys Phe Arg Asp Ala Ile His Pro 
            100                 105                 110         


Lys Thr Leu Ala Thr Glu Thr Val Ser Glu Thr Lys Pro His Glu Glu 
        115                 120                 125             


Glu Leu Pro Asp Val Ser Ser Thr Thr Ala Gly Thr Thr Met Ala Thr 
    130                 135                 140                 


Ala Thr Val Met Glu Thr Ala Thr Thr Val Lys Glu Lys Thr His Arg 
145                 150                 155                 160 


Arg Thr Phe His Ile Pro Gln Glu Ile Lys Gly Pro Ala Val Ala Ala 
                165                 170                 175     


Phe Val Val Ser Val Ile Asn Pro Ala Ala Trp Val Asp Leu Phe Val 
            180                 185                 190         


Val Ile Gly Ser Ile Ser Ser Ser Tyr Gly Pro Asp Lys Trp Ala Phe 
        195                 200                 205             


Leu Leu Gly Thr Met Ala Ala Ser Leu Val Trp Phe Pro Ala Phe Gly 
    210                 215                 220                 


Tyr Gly Ala Ala Ala Leu Ser Arg Pro Leu Ser Ser Pro Lys Val Trp 
225                 230                 235                 240 


Arg Cys Ile Asn Thr Gly Ile Gly Leu Phe Met Val Phe Met Ala Phe 
                245                 250                 255     


Arg Val Leu Phe Met 
            260     


<210>  17
<211>  204
<212>  PRT
<213>  Micrococcus luteus NCTC2665

<400>  17

Met Trp Thr Leu Ala Gly Thr Gly Leu Leu Thr Gly Leu Ala Leu Ile 
1               5                   10                  15      


Val Ala Ile Gly Ala Gln Asn Ala Phe Val Leu Arg Gln Gly Val Arg 
            20                  25                  30          


Arg Glu His Val Gly Ala Val Val Leu Val Cys Met Ala Ser Asp Ala 
        35                  40                  45              


Val Leu Ile Leu Ala Gly Thr Ala Gly Val Gly Ala Leu Val Gln Ala 
    50                  55                  60                  


Val Pro Trp Leu Leu Glu Val Leu Arg Trp Gly Gly Ala Leu Tyr Leu 
65                  70                  75                  80  


Leu Trp Phe Ala Val Ser Ser Leu Arg Ala Ala Leu Arg Pro Gln Gly 
                85                  90                  95      


Leu Met Ala Glu Gln Ala Pro Arg Thr Ala Gly Ser Val Ile Ala Thr 
            100                 105                 110         


Thr Leu Ala Leu Thr Trp Leu Asn Pro His Val Tyr Leu Asp Thr Val 
        115                 120                 125             


Val Leu Leu Gly Ser Leu Ala Asn Gln His Gly Pro Asp Ala Arg Trp 
    130                 135                 140                 


Val Phe Ala Ala Gly Ala Val Ala Ala Ser Val Leu Trp Phe Thr Ala 
145                 150                 155                 160 


Leu Gly Tyr Gly Ala Arg Leu Leu Ala Arg Val Leu Ala Asp Pro Lys 
                165                 170                 175     


Ala Trp Arg Val Val Asp Val Val Ile Ala Val Val Met Ala Val Leu 
            180                 185                 190         


Ala Val Arg Leu Ile Ala Gly Ser Asp Val Trp Gly 
        195                 200                 


<210>  18
<211>  230
<212>  PRT
<213>  Corynebacterium tubuculostearicum SK141


<220>
<221>  ArgO(LysE)
<222>  (1)..(230)

<400>  18

Met Ser Ile Val Leu Ala Gly Phe Phe Leu Gly Leu Ser Leu Ile Val 
1               5                   10                  15      


Ala Val Gly Pro Gln Asn Ala Met Leu Leu Lys Tyr Gly Ile Arg Arg 
            20                  25                  30          


Asp His Ile Gly Leu Ile Ile Val Val Cys Ala Leu Ser Asp Val Ile 
        35                  40                  45              


Leu Ile Thr Ser Gly Thr Ala Gly Val Gly Tyr Leu Val Glu Lys Phe 
    50                  55                  60                  


Pro Asn Ala Leu Gln Val Leu Lys Tyr Val Gly Ala Ala Tyr Leu Ala 
65                  70                  75                  80  


Tyr Phe Thr Phe Thr Cys Phe Arg Asp Ala Leu Lys Thr Lys Gly Glu 
                85                  90                  95      


Ala Ile Glu Val Glu Ser Thr Gln Pro Lys Val Pro Gln Glu Val Ala 
            100                 105                 110         


Ser Phe Asp Gly Ser Gln Ala Arg Asn Thr Thr Lys Thr Ala Thr Arg 
        115                 120                 125             


Val Glu Ile Lys Arg Ser Pro Ser Trp Val Lys Pro Leu Leu Thr Ala 
    130                 135                 140                 


Leu Ala Leu Thr Trp Leu Asn Pro Gly Ala Tyr Val Asp Val Val Val 
145                 150                 155                 160 


Met Leu Gly Ser Ile Ala Asn Gln Tyr Gly Glu Ser Gly Arg Trp Leu 
                165                 170                 175     


Phe Ala Val Gly Ala Ile Cys Ala Ser Phe Thr Trp Phe Pro Phe Ile 
            180                 185                 190         


Gly Phe Gly Ala Ala Arg Phe Ser His Val Leu Ser Arg Pro Thr Val 
        195                 200                 205             


Trp Arg Trp Ile Asn Phe Gly Ile Gly Val Ile Met Ile Gly Leu Thr 
    210                 215                 220                 


Leu Lys Leu Leu Leu Leu 
225                 230 


<210>  19
<211>  244
<212>  PRT
<213>  Corynebacterium matruchotii ATCC14266


<220>
<221>  ArgO(LysE)
<222>  (1)..(244)

<400>  19

Met Ser Ile Ala Val Ala Gly Phe Leu Leu Gly Leu Ser Leu Ile Val 
1               5                   10                  15      


Ala Ile Gly Pro Gln Asn Ala Leu Val Ile Arg Gln Gly Val Lys Arg 
            20                  25                  30          


Glu Gly Leu Ile Val Val Leu Ala Ile Cys Met Leu Ser Asp Ile Phe 
        35                  40                  45              


Leu Ile Phe Gly Gly Thr Ala Gly Val Gly Val Ile Ile Glu Lys Ala 
    50                  55                  60                  


Pro Leu Ala Leu Val Ala Leu Lys Trp Phe Gly Ala Ala Tyr Leu Ala 
65                  70                  75                  80  


Trp Phe Ala Val Ser Cys Phe Lys Asp Met Val Lys Pro Arg Ala Leu 
                85                  90                  95      


Asp Ser Ser Ala Thr Asp Asn Gly Thr Ser Leu Asp Asp Ala Pro Thr 
            100                 105                 110         


Val Ala His Ile Ser Asn Val Asp Ser Thr Ser Gly Asn Gly Gly Gln 
        115                 120                 125             


Val Gln Thr Lys Thr Arg Pro Ile Thr Thr Thr Ala Pro Thr Arg Gln 
    130                 135                 140                 


Ala His Pro Ala Arg Pro Trp Val Lys Pro Ala Leu Ala Ala Leu Ala 
145                 150                 155                 160 


Phe Thr Trp Leu Asn Pro Ser Ala Tyr Ile Asp Thr Leu Val Met Leu 
                165                 170                 175     


Gly Gly Ile Ala Asn Gln His Gly Glu Ser Gly Arg Trp Val Phe Ala 
            180                 185                 190         


Ala Gly Ala Leu Met Ala Ser Ala Val Trp Phe Pro Leu Leu Gly Phe 
        195                 200                 205             


Phe Ser Thr Arg Phe Ser Arg Val Leu Ser Arg Pro Gln Ala Trp Arg 
    210                 215                 220                 


Val Ile Asn Gly Val Ile Gly Cys Ile Met Val Val Met Cys Ile Arg 
225                 230                 235                 240 


Leu Ile Met His 
                


<210>  20
<211>  864
<212>  DNA
<213>  Corynebacterium glutamicum ATCC13032


<220>
<221>  lysE
<222>  (76)..(777)
<223>  pVWEx1- Insert

<400>  20
tgagcggata acaatttcac acaggaaaca gaattaaaag atatgaccat gattacgcca     60

agcttgcatg ccatcatgga aatcttcatt acaggtctgc ttttgggggc cagtctttta    120

ctgtccatcg gaccgcagaa tgtactggtg attaaacaag gaattaagcg cgaaggactc    180

attgcggttc ttctcgtgtg tttaatttct gacgtctttt tgttcatcgc cggcaccttg    240

ggcgttgatc ttttgtccaa tgccgcgccg atcgtgctcg atattatgcg ctggggtggc    300

atcgcttacc tgttatggtt tgccgtcatg gcagcgaaag acgccatgac aaacaaggtg    360

gaagcgccac agatcattga agaaacagaa ccaaccgtgc ccgatgacac gcctttgggc    420

ggttcggcgg tggccactga cacgcgcaac cgggtgcggg tggaggtgag cgtcgataag    480

cagcgggttt gggtaaagcc catgttgatg gcaatcgtgc tgacctggtt gaacccgaat    540

gcgtatttgg acgcgtttgt gtttatcggc ggcgtcggcg cgcaatacgg cgacaccgga    600

cggtggattt tcgccgctgg cgcgttcgcg gcaagcctga tctggttccc gctggtgggt    660

ttcggcgcag cagcattgtc acgcccgctg tccagcccca aggtgtggcg ctggatcaac    720

gtcgtcgtgg cagttgtgat gaccgcattg gccatcaaac tgatgttgat gggttagttt    780

tcgcgggttt tggaatcggt ggccttcgcc caaatattga cctagaggat ccccgggtac    840

cgagctcgaa ttcactggcc gtcg                                           864


<210>  21
<211>  1036
<212>  DNA
<213>  Corynebacterium glutamicum ATCC13032


<220>
<221>  'argD
<222>  (15)..(491)
<223>  3'-Ende des argD-Gens

<220>
<221>  argF'
<222>  (505)..(523)
<223>  5'-Ende des argF-Gens

<220>
<221>  'argH
<222>  (524)..(538)
<223>  3'-Ende des argH-Gens

<220>
<221>  cg1589'
<222>  (605)..(1024)
<223>  5'-Ende des cg1589-Leserasters

<400>  21
ggtggtgcta gcccggcgat ttctttgcac atcagcacga tggcgttgtt cccgatgtgg     60

tgaccatggc caagggactt ggcggcggtc ttcccatcgg tgcttgtttg gccactggcc    120

gtgcagctga attgatgacc ccaggcaagc acggcaccac tttcggtggc aacccagttg    180

cttgtgcagc tgccaaggca gtgctgtctg ttgtcgatga cgctttctgc gcagaagttg    240

cccgcaaggg cgagctgttc aaggaacttc ttgccaaggt tgacggcgtt gtagacgtcc    300

gtggcagggg cttgatgttg ggcgtggtgc tggagcgcga cgtcgcaaag caagctgttc    360

ttgatggttt taagcacggc gttattttga atgcaccggc ggacaacatt atccgtttga    420

ccccgccgct ggtgatcacc gacgaagaaa tcgcagacgc agtcaaggct attgccgaga    480

caatcgcata aaggactcaa acttatgact tcacaaccac aggggggtac gtcgataagc    540

attagtttat ggcctgtgct gctttccgat tgcgggagtt gcacaggcca tttattatca    600

attcatgaat ggttcccctg attactcaag aaaatctcga ggcaggggat tttccgtatt    660

tttaggcatc atcctgctgg tcatcgcggt attagcggtc ttggtgggcc gcggaaccat    720

cgccatgcca aagctcttcg gatcaagtaa cttgaccgag gtcagagcag taattggctc    780

ggaaaagaag gaattcttcg aagatccaga agtcgttgaa gccttcgccg accacggctt    840

tgaagttaac gtggacaccg caggatctcg tcggatcgcc actgatgtgg acttgagccc    900

gtacgatttc gcgttcccat cctctgcacc agctgcgcag aagatctccg aagccaacac    960

cacgacgggc cgattcaccc cattctattc gcctatggcg gtagcaacat tcggaaccat   1020

caccatgcat accacc                                                   1036


<210>  22
<211>  28
<212>  DNA
<213>  Oligonukleotid


<220>
<221>  lysE_1.p
<222>  (1)..(28)

<400>  22
tcgatatcat ggaaatcttc attacagg                                        28


<210>  23
<211>  32
<212>  DNA
<213>  Oligonukleotid


<220>
<221>  lysE_2.p
<222>  (1)..(32)

<400>  23
tgcctaggtc aatatttggg cgaaggccac cg                                   32


<210>  24
<211>  32
<212>  DNA
<213>  Oligonukleotid


<220>
<221>  argFRGH_d1
<222>  (1)..(32)

<400>  24
ggtggtgcta gcccggcgat ttctttgcac at                                   32


<210>  25
<211>  40
<212>  DNA
<213>  Oligonukleotid


<220>
<221>  argFRGH_d2
<222>  (1)..(40)

<400>  25
aatgcttatc gacgtacccc cctgtggttg tgaagtcata                           40


<210>  26
<211>  20
<212>  DNA
<213>  Oligonukleotid


<220>
<221>  argFRGH_d3
<222>  (1)..(20)

<400>  26
ggggtacgtc gataagcatt                                                 20


<210>  27
<211>  32
<212>  DNA
<213>  Oligonukleotid


<220>
<221>  argFRGH_d4
<222>  (1)..(32)

<400>  27
ggtggtatgc atggtgatgg ttccgaatgt tg                                   32


<210>  28
<211>  238
<212>  DNA
<213>  PgapRBS Corynebacterium glutamicum ATCC13032


<220>
<221>  RBS
<222>  (225)..(232)

<400>  28
ctgtatgatt ttgcatctgc tgcgaaatct ttgtttcccc gctaaagttg aggacaggtt     60

gacacggagt tgactcgacg aattatccaa tgtgagtagg tttggtgcgt gagttggaaa    120

aattcgccat actcgccctt gggttctgtc agctcaagaa ttcttgagtg accgatgctc    180

tgattgacct aactgcttga cacattgcat ttcctacaat ctttagagga gacacaac      238


<210>  29
<211>  8281
<212>  DNA
<213>  pEC7lysE

<400>  29
tcccagaggc agcgtcggcg gctcctgcct cccccgcccg gcgccgaccg ggacccgcaa     60

accccttgat ccgctgtcgg gggtgatcct gcaagcctcg tcgtcctggc cggaccacgc    120

tatctgtgca aggtccccgg ccccggacgc gcgctccatg agcagagcgc ccgccgggga    180

tcgatccggg cttatcgact gcacggtgca ccaatgcttc tggcgtcagg cagccatcgg    240

aagctgtggt atggctgtgc aggtcgtaaa tcactgcata attcgtgtcg ctcaaggcgc    300

actcccgttc tggataatgt tttttgcgcc gacatcataa cggttctggc aaatattctg    360

aaatgagctg ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa    420

caatttcaca caggaaacag aattcccagc ttgagtagga caaatccgcc gagcttcgac    480

gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt    540

tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg    600

tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa    660

taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc    720

ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg    780

ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga    840

cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct    900

ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt    960

gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt   1020

caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt   1080

tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta   1140

ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac   1200

gcctggttgc tacgcctgaa taagtgataa taagcggatg aatggcagaa attcgtcgag   1260

gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag   1320

aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag   1380

cagccgcacg cggcgcatct cggctgtttt ggcggatgag agaagatttt cagcctgata   1440

cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc   1500

gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt   1560

agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc   1620

tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag   1680

taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg   1740

ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga   1800

tggccttttt gcgtttctac aaactcttcc tgtcgtcata tctacaagcc atccccccac   1860

agatacggta aactagcctc gtttttgcat caggaaagca gaattcgtaa tcatgtcata   1920

gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag   1980

cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg   2040

ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca   2100

acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc   2160

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg   2220

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa   2280

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga   2340

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag   2400

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct   2460

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg   2520

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc   2580

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt   2640

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta   2700

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac   2760

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc   2820

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat   2880

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc   2940

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt   3000

cacctagatc cttttggggg gggggggaaa gccacgttgt gtctcaaaat ctctgatgtt   3060

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca   3120

gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aagcccgcct   3180

aatgagcggg ctttttttta aaaaaaagcc cgctcattag gcgggctagc ccgcctaatg   3240

agcgggcttt ttttttcgag gccgcgatta aattccaaca tggatgctga tttatatggg   3300

tataaatggg ctcgaaaaga tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg   3360

actctagagg atccaccgtg accagtgcat tgattggtgc ttcgtcagtt gagcagctgg   3420

acaacagcct tgattcactc aacaacttgg agttttctga cgccgagttg gaggcgatcg   3480

atgagatttc ccacgacgcc ggcatcaaca tttgggcgaa ggccaccgat tccaaaaccc   3540

gcgaaaacta acccatcaac atcagtttga tggccaatgc ggtcatcaca actgccacga   3600

cgacgttgat ccagcgccac accttggggc tggacagcgg gcgtgacaat gctgctgcgc   3660

cgaaacccac cagcgggaac cagatcaggc ttgccgcgaa cgcgccagcg gcgaaaatcc   3720

accgtccggt gtcgccgtat tgcgcgccga cgccgccgat aaacacaaac gcgtccaaat   3780

acgcattcgg gttcaaccag gtcagcacga ttgccatcaa catgggcttt acccaaaccc   3840

gctgcttatc gacgctcacc tccacccgca cccggttgcg cgtgtcagtg gccaccgccg   3900

aaccgcccaa aggcgtgtca tcgggcacgg ttggttctgt ttcttcaatg atctgtggcg   3960

cttccacctt gtttgtcatg gcgtctttcg ctgccatgac ggcaaaccat aacaggtaag   4020

cgatgccacc ccagcgcata atatcgagca cgatcggcgc ggcattggac aaaagatcaa   4080

cgcccaaggt gccggcgatg aacaaaaaga cgtcagaaat taaacacacg agaagaaccg   4140

caatgagtcc ttcgcgctta attccttgtt taatcaccag tacattctgc ggtccgatgg   4200

acagtaaaag actggccccc aaaagcagac ctgtaatgaa gatttccatg atcaccatcg   4260

tgacctatgg aagtacttaa gtaaaatgat tggttcttaa catggtttaa tatagcttca   4320

tgaaccccat tcaactggac actttgctct caatcattga tgaaggcagc ttcgaaggcg   4380

cctccttagc cctttccatt tccccctcgg cggtgagtca gcgcggatcc ccgggtaccg   4440

agctcgaatt cactggccgt cgttttaccg ataatgtcgg gcaatcaggt gcgacaatct   4500

atcgagcttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat   4560

aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc   4620

gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca   4680

ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag   4740

tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg   4800

cagagcatta cgctgacttg acgggacggc ggctttgttg aataaatcga acttttgctg   4860

agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa   4920

agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt ggctccctca   4980

ctttctggct ggatgatggg gcgattcagg cctggtatga gtcagcaaca ccttcttcac   5040

gaggcagacc tcagcgcccc ccccccccta gcttgtctac gtctgatgct ttgaatcgga   5100

cggacttgcc gatcttgtat gcggtgattt ttccctcgtt tgcccacttt ttaatggtgg   5160

ccggggtgag agctacgcgg gcggcgacct gctgcgctgt gatccaatat tcggggtcgt   5220

tcactggttc ccctttctga tttctggcat agaagaaccc ccgtgaactg tgtggttccg   5280

ggggttgctg atttttgcga gacttctcgc gcaattccct agcttaggtg aaaacaccat   5340

gaaacactag ggaaacaccc atgaaacacc cattagggca gtagggcggc ttcttcgtct   5400

agggcttgca tttgggcggt gatctggtct ttagcgtgtg aaagtgtgtc gtaggtggcg   5460

tgctcaatgc actcgaacgt cacgtcattt accgggtcac ggtgggcaaa gagaactagt   5520

gggttagaca ttgttttcct cgttgtcggt ggtggtgagc ttttctagcc gctcggtaaa   5580

cgcggcgatc atgaactctt ggaggttttc accgttctgc atgcctgcgc gcttcatgtc   5640

ctcacgtagt gccaaaggaa cgcgtgcggt gaccacgacg ggcttagcct ttgcctgcgc   5700

ttctagtgct tcgatggtgg cttgtgcctg cgcttgctgc gcctgtagtg cctgttgagc   5760

ttcttgtagt tgctgttcta gctgtgcctt ggttgccatg ctttaagact ctagtagctt   5820

tcctgcgata tgtcatgcgc atgcgtagca aacattgtcc tgcaactcat tcattatgtg   5880

cagtgctcct gttactagtc gtacatactc atatttacct agtctgcatg cagtgcatgc   5940

acatgcagtc atgtcgtgct aatgtgtaaa acatgtacat gcagattgct gggggtgcag   6000

ggggcggagc caccctgtcc atgcggggtg tggggcttgc cccgccggta cagacagtga   6060

gcaccggggc acctagtcgc ggataccccc cctaggtatc ggacacgtaa ccctcccatg   6120

tcgatgcaaa tctttaacat tgagtacggg taagctggca cgcatagcca agctaggcgg   6180

ccaccaaaca ccactaaaaa ttaatagttc ctagacaaga caaacccccg tgcgagctac   6240

caactcatat gcacgggggc cacataaccc gaaggggttt caattgacaa ccatagcact   6300

agctaagaca acgggcacaa cacccgcaca aactcgcact gcgcaacccc gcacaacatc   6360

gggtctaggt aacactgaaa tagaagtgaa cacctctaag gaaccgcagg tcaatgaggg   6420

ttctaaggtc actcgcgcta gggcgtggcg taggcaaaac gtcatgtaca agatcaccaa   6480

tagtaaggct ctggcggggt gccataggtg gcgcagggac gaagctgttg cggtgtcctg   6540

gtcgtctaac ggtgcttcgc agtttgaggg tctgcaaaac tctcactctc gctgggggtc   6600

acctctggct gaattggaag tcatgggcga acgccgcatt gagctggcta ttgctactaa   6660

gaatcacttg gcggcgggtg gcgcgctcat gatgtttgtg ggcactgttc gacacaaccg   6720

ctcacagtca tttgcgcagg ttgaagcggg tattaagact gcgtactctt cgatggtgaa   6780

aacatctcag tggaagaaag aacgtgcacg gtacggggtg gagcacacct atagtgacta   6840

tgaggtcaca gactcttggg cgaacggttg gcacttgcac cgcaacatgc tgttgttctt   6900

ggatcgtcca ctgtctgacg atgaactcaa ggcgtttgag gattccatgt tttcccgctg   6960

gtctgctggt gtggttaagg ccggtatgga cgcgccactg cgtgagcacg gggtcaaact   7020

tgatcaggtg tctacctggg gtggagacgc tgcgaaaatg gcaacctacc tcgctaaggg   7080

catgtctcag gaactgactg gctccgctac taaaaccgcg tctaaggggt cgtacacgcc   7140

gtttcagatg ttggatatgt tggccgatca aagcgacgcc ggcgaggata tggacgctgt   7200

tttggtggct cggtggcgtg agtatgaggt tggttctaaa aacctgcgtt cgtcctggtc   7260

acgtggggct aagcgtgctt tgggcattga ttacatagac gctgatgtac gtcgtgaaat   7320

ggaagaagaa ctgtacaagc tcgccggtct ggaagcaccg gaacgggtcg aatcaacccg   7380

cgttgctgtt gctttggtga agcccgatga ttggaaactg attcagtctg atttcgcggt   7440

taggcagtac gttctagatt gcgtggataa ggctaaggac gtggccgctg cgcaacgtgt   7500

cgctaatgag gtgctggcaa gtctgggtgt ggattccacc ccgtgcatga tcgttatgga   7560

tgatgtggac ttggacgcgg ttctgcctac tcatggggac gctactaagc gtgatctgaa   7620

tgcggcggtg ttcgcgggta atgagcagac tattcttcgc acccactaaa agcggcataa   7680

accccgttcg atattttgtg cgatgaattt atggtcaatg tcgcgggggc aaactatgat   7740

gggtcttgtt gttgacaatg gctgatttca tcaggaatgg aactgtcatg ctgttatgtg   7800

cctggctcct aatcaaagct ggggacaatg ggttgccccg ttgatctgat ctagttcgga   7860

ttggcggggc ttcactgtat ctgggggtgg catcgtgaat agattgcaca ccgtagtggg   7920

cagtgtgcac accatagtgg ccatgagcac caccaccccc agggacgccg acggcgcgaa   7980

gctctgcgcc tggtgcggct cggagatcaa gcaatccggc gtcggccgga gccgggacta   8040

ctgccgccgc tcctgccgcc agcgggcgta cgaggcccgg cgccagcgcg aggcgatcgt   8100

gtccgccgtg gcgtcggcag tcgctcgccg agatacgtca cgtgacgaaa tgcagcagcc   8160

ttccattccg tcacgtgacg aaactcgggc cgcaggtcag agcacggttc cgcccgctcc   8220

ggccctgccg gacccccggc tgcagctcgc ccggccgccg gtccccctgc cgtccggccc   8280

g                                                                   8281


<210>  30
<211>  290
<212>  PRT
<213>  Corynebacterium glutamicum ATCC13032


<220>
<221>  misc_feature
<222>  (1)..(290)
<223>  Aminosuresequenz des Aktivatorproteins LysG von Corynebacterium 
       glutamicum ATCC13032

<400>  30

Met Asn Pro Ile Gln Leu Asp Thr Leu Leu Ser Ile Ile Asp Glu Gly 
1               5                   10                  15      


Ser Phe Glu Gly Ala Ser Leu Ala Leu Ser Ile Ser Pro Ser Ala Val 
            20                  25                  30          


Ser Gln Arg Val Lys Ala Leu Glu His His Val Gly Arg Val Leu Val 
        35                  40                  45              


Ser Arg Thr Gln Pro Ala Lys Ala Thr Glu Ala Gly Glu Val Leu Val 
    50                  55                  60                  


Gln Ala Ala Arg Lys Met Val Leu Leu Gln Ala Glu Thr Lys Ala Gln 
65                  70                  75                  80  


Leu Ser Gly Arg Leu Ala Glu Ile Pro Leu Thr Ile Ala Ile Asn Ala 
                85                  90                  95      


Asp Ser Leu Ser Thr Trp Phe Pro Pro Val Phe Asn Glu Val Ala Ser 
            100                 105                 110         


Trp Gly Gly Ala Thr Leu Thr Leu Arg Leu Glu Asp Glu Ala His Thr 
        115                 120                 125             


Leu Ser Leu Leu Arg Arg Gly Asp Val Leu Gly Ala Val Thr Arg Glu 
    130                 135                 140                 


Ala Asn Pro Val Ala Gly Cys Glu Val Val Glu Leu Gly Thr Met Arg 
145                 150                 155                 160 


His Leu Ala Ile Ala Thr Pro Ser Leu Arg Asp Ala Tyr Met Val Asp 
                165                 170                 175     


Gly Lys Leu Asp Trp Ala Ala Met Pro Val Leu Arg Phe Gly Pro Lys 
            180                 185                 190         


Asp Val Leu Gln Asp Arg Asp Leu Asp Gly Arg Val Asp Gly Pro Val 
        195                 200                 205             


Gly Arg Arg Arg Val Ser Ile Val Pro Ser Ala Glu Gly Phe Gly Glu 
    210                 215                 220                 


Ala Ile Arg Arg Gly Leu Gly Trp Gly Leu Leu Pro Glu Thr Gln Ala 
225                 230                 235                 240 


Ala Pro Met Leu Lys Ala Gly Glu Val Ile Leu Leu Asp Glu Ile Pro 
                245                 250                 255     


Ile Asp Thr Pro Met Tyr Trp Gln Arg Trp Arg Leu Glu Ser Arg Ser 
            260                 265                 270         


Leu Ala Arg Leu Thr Asp Ala Val Val Asp Ala Ala Ile Glu Gly Leu 
        275                 280                 285             


Arg Pro 
    290 



1
200900319 Ausland

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