                         SEQUENCE LISTING

<110>  Marquette University
       Zitomer, Daniel H.
 
<120>  PRESERVATION OF METHANOGENIC, HYDROGEN-UTILIZING MICROBIAL 
       CULTURES

<130>  5528-00030

<150>  US 61/341,705
<151>  2010-04-04

<150>  US 61/396,337
<151>  2010-05-26

<150>  US 61/396,339
<151>  2010-05-26

<150>  US 61/396,340
<151>  2010-05-26

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  1466
<212>  DNA
<213>  Methanospirillum hungatei


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or t

<400>  1
attctgnttg atcctgccag aggccactgc tatcggggtt tgactaagcc atgcgagtcg       60

agaggtgcaa gacctcggcg tactgctcag taacacgtgg acaatctgcc ctgaagagga      120

ggataatccc gggaaactgg gggtaatact ccatagttcg tgctgactgg aatgttatgc      180

gaacgaaaga tccgtcgctt caggatgagt ctgcggccga ttaggtagtt gttggggtaa      240

cggcccaaca agcctgtcat cggtacgggt tgtgggagca agagcccgga gatggattct      300

gagacacgaa tccaggccct acggggcgca gcaggcgcga aaactttacc atgcgggcaa      360

ccgtgataag gaaaccccga gtgccagcac aggctggctg tccaccagtg taaataactg      420

gtgaagaaag ggccgggcaa gaccggtgcc agccgccgcg gtaataccgg cggctcgagt      480

ggtggccgct attactgggc ttaaagggtc cgtagctgga tatacaagtc ccttgagaaa      540

tccgccggct taaccggtgg gcgttcaggg gaaactgtat ttctagggac cgggagaggt      600

gagaggtact gccggggtag gagtgaaatc ctgtaatccc ggtgggacca cctatggcga      660

aggcatctca ccagaacggg tccgacagtg agggacgaaa gctgggggag caaaccggat      720

tagatacccg ggtagtccca gctgtaaacg atgcgcgtta ggtgtgtcag tgaccacgtg      780

tcactgaggt gccgaaggga aaccgtgaaa cgcgccgcct ggggagtacg gtcgcaaggc      840

tgaaacttaa aggaattggc gggggagcac cacaacgggt ggagcctgcg gtttaatcgg      900

actcaacgcc ggaaatctca ccggataaga cagctgaatg atagtcggga tgaagactct      960

acttgactag ctgagaggag gtgcatggcc gtcgtcagtt cgtactgtga agcatcctgt     1020

ttagtcaggc aacgagcgag acccacgcga gcagttgcca gcttgacctt cgggttgatg     1080

gggacactgc tcggaccgcc tctgctaaag gggaggaagg aatgggcaac ggtaggtcag     1140

catgccccga attatccggg ctacacgcgg gctacaatgg acaggacaat gggtttcgac     1200

accgagaggt gaggataatc tcctaaacct gtccgaagtt cggattgcgg gttgtaactc     1260

acccgcatga agctggaatc cgtagtaatc gcgtttcaac atagcgcggt gaatatgtcc     1320

ctgctccttg cacacaccgc ccgtcaaacc acccgagtga ggtcttgatg aggatgtatc     1380

attgatatgt tcgaatctgg gttttgcaag gggggttaag tcgtaacaag gtagccgtag     1440

gggaatctgc ggctggatca cctcct                                          1466


<210>  2
<211>  743
<212>  DNA
<213>  Methanospirillum hungatei

<400>  2
cttcattacc gcataccgca tgtgtgccgg agaagcagca gtcgctgacc tgtcctttgc       60

agcaaagcac gctggtgtta tccagatggc aagtcacctc ccggcccgtc gtgcccgtgg      120

tccaaatgaa ccaggaggta tcatgttcgg acactttgct gacatgatcc aggcaaaccg      180

gaagtacccg aatgacccag caaaggcatc acttgaggtt gtcggtgcag gttgtatgct      240

cttcgaccag atctggctcg gttcctacat gtctggtggt gtcggattta cccagtatgc      300

aaccgcagca tacaccgaca acatcctcga tgagttcacc tactatggta tggactacat      360

caaggacaag tacaaagtcg actggaagaa cccaagcccg aaagacaagg tcaagccaac      420

ccaggagatc gtcaacgaca ttgccggaga ggtcaccctc aatgcaatgg agcagtacga      480

acagttccca accatgatgg aagaccactt tggtggttcc cagcgtgcag gagttatcgc      540

agcagcatcc ggtctgtctg tcggtgtcgc aacagcaaac tccaacgcag gtctgaacgg      600

atggtacctc tccatgctca tgcacaagga aggctggtca cgtctcggat tcttcggata      660

cgacctgcag gaccagtgtg gttccaccaa ctcactctct gtcagacctg acgagggttg      720

tatcggtgaa taccgtggtc cta                                              743


<210>  3
<211>  1433
<212>  DNA
<213>  Methanolinea tarda

<400>  3
ttccggttga tcctgccgga ggccactgct atcggggttc gattaagcca tgcgagtcga       60

gaggtgcaag acctcggcgc actgctcagt aacacgtgga taacctaccc tcaggtgggg      120

gataaccccg ggaaactggg gataataccc catagaccag ggacgctgga atgccccctg      180

atcgaaaggt ccgccgcctg aggatgggtc tgcggccgat taggttgttg ttggggtaac      240

ggcccaacaa gcctttgatc ggtacgggtt gtgagagcaa gagcccggag atggattctg      300

agacacaaat ccaggcccta cggggcgcag caggcgcgaa aactttacaa tgcgagaaat      360

cgtgataagg gaaccccgag tgcccgtaaa ttcgggctgt ccatcagcgt aaaaaactgg      420

tgaagaaagg gccgggcaag accggtgcca gccgccgcgg taataccggc ggctcgagtg      480

gtggccacta ttactgggct taaagcgtcc gtagctggat tgttaagtct cttgggaaat      540

ccgccggctt aaccggcggg cgttcaggag aaactggcaa tctagggacc gggagaggtg      600

agaggtactc caggggtagg agtgaaatcc tgtaatcctt gggggaccac ctgtggcgaa      660

ggcgtctcac tagaacggct ccgacagtga gggacgaaag ctgggggagc aaaccggatt      720

agatacccgg gtagtcccag ctgtaaacga tgcgcgttag gtgtatcggt gaccacgagt      780

catcgaggtg ccgaagggaa accgtgaaac gtgccgcctg ggaagtacgg tcgcaaggct      840

gaaacttaaa ggaattggcg ggggagcacc acaacgggtg gagcctgcgg tttaattgga      900

ctcaacgccg ggaagctcac cggataagac agctggatga tagccgggct gaagactctg      960

cttgactagc tgagaggagg tgcatggccg tcgtcagttc gtactgtgaa gcatcctgtt     1020

aagtcaggca acgagcgaga cccacgccaa cagttgccag cgtatcctcc gggatgacgg     1080

ggacactgtt gggaccgcct ctgctaaaga ggaggaagga atgggcaacg gtaggtcagc     1140

atgccccgaa ttatccgggc tacacgcggg ctacaatggt caggacaatg ggtatcgaca     1200

ccgagaggtg aaggcaatct cctaaacctg atcgtagttc ggattgtggg ctgcaactcg     1260

cccacatgaa gctggaatcc gtagtaatcg cgtttcaaaa tagcgcggtg aatatgtccc     1320

tgctccttgc acacaccgcc cgtcaaacca cccgagtggg gtcttgatga ggctgcggtt     1380

gccgccgtgg tcgaatctag gttccgcaag gggggttaag tcgtaacaag gta            1433


<210>  4
<211>  763
<212>  DNA
<213>  Methanolinea tarda

<400>  4
gccatgcaga ttggtatgtc cttcatcggt gcctaccgca tgtgcgccgg tgaggcggcg       60

accgctgacc ttgcattcgc agcaaagcac gccggtgtca tccagatggg tgagatcctg      120

cctgcacgcc gtgcccgtgg cccgaacgag cccggtggca tcaagttcgg acactttgcc      180

gacatggtcc agacggacag gaagtacccg aacgaccccg cacgcgcctc cctcgaggtc      240

gtgggtgcag ggacgatgct ctttgaccag atctggctcg ggtcctacat gtccggcggt      300

gtcgggttca cgcagtacgc aactgccgcc tacaccgaca acatcctcga tgactatacc      360

tactacggta tggactacat caagcagaaa tacaaagtcg actggcagaa cccgaacgag      420

aaggacaagg tcaagccgac ccaggacatc gtcaacgaca tcgcaacgga ggtcaccctc      480

tacggcatgg agcagtacga gcacttcccg actgcactcg aggaccactt cggcggttcc      540

cagcgtgcgt cggtccttgc tgctgcatcc ggtctcacga ccgcaattgc cacagggaac      600

tccaatgccg gactgaacgg ctggtacctg tccatgctcc tgcacaagga gggctggtca      660

cggctcggct tctacggata cgacctgcag gaccagtgcg gttccgcaaa caccgagtcc      720

atccgtgcag acgagggttg tgtcggagag ctccgcgggc cca                        763


<210>  5
<211>  1349
<212>  DNA
<213>  Methanobacterium beijingense

<400>  5
ttccggttga tcctgccgga ggccactgct attggggtcc gattaagcca tgcaagtcga       60

acgttcttcg gaacgtggca aacggctcag taacacgtgg ataacctacc cttaggaccg      120

ggataaccct gggaaactgg ggataatacc ggatatatgg agatacctgg aatggttctc      180

cacttaaagc tccggcgcct aaggatggat ctgcggcaga ttaggtcgtt ggtggggtaa      240

tggcccacca agcctttgat ctgtacgggt tgtgagagca agagcccgga gatggaacct      300

gagacaaggt tccaggccct acggggcgca gcaggcgcga aacctccgca atgcgagcaa      360

tcgcgacggg gggaccccaa gtgccactct taacggggtg gcttttctta agtgtaaaaa      420

gcttttggaa taagggctgg gcaagaccgg tgccagccgc cgcggtaaca ccggcagccc      480

aagtggtggc catttttatt gggcctaaag cgttcgtagc cggcctgata agtctctggt      540

gaaatcccgc agcttaactg tgggaattgc tggagatact atcaggcttg aggtcgggag      600

aggttagagg tactcccagg gtaggggtga aatcctataa tcctgggagg accacctgtg      660

gcgaaggcgt ctaactggaa cgaacctgac ggtgagtaac gaaagccagg ggcgcgaacc      720

ggattagata cccgggtagt cctggccgta aacgatgtgg acttggtgtt gggatggcct      780

cgagctgccc cagtgccgaa gggaagctgt taagtccacc gcctgggaag tacggtcgca      840

agactgaaac ttaaaggaat tggcggggga gcaccacaac gcgtggagcc tgcggtttaa      900

atggattcaa cgccggacat ctcaccaggg gcgacagcag gatgatggcc agattgacga      960

tcttgcttga caagctgaga ggaggtgcat ggccgccgtc agctcgtacc gtgaggcgtc     1020

ctgttaagtc aggcaacgag cgagacccac gcccttagtt accagcggat ccttacagga     1080

tgccgggcac actaagggga ccgccagtga taaactggag gaaggagtgg acgacggtag     1140

gtccgtatgc cccgaatccc ctgggctaca cgcgggctac aatggctagg acaatgggtt     1200

ccaacactga aaagtgaagg taatctccta aacctagtct tagttcggat tgagggctgt     1260

aactcgccct catgaagctg gaatgcgtag taatcgcgtg tcataatcgc gcggtgaata     1320

cgtccctgct ccttgcacac accgcccgt                                       1349


<210>  6
<211>  493
<212>  DNA
<213>  Methanobacterium beijingense

<400>  6
atgatcagat ttggctaggt tcatacatgt ctggcggtgt aggattcacc cagtacgcaa       60

ccgcagcata caccgacaac atactggacg acttcaccta ctttggtaaa gagtacgtag      120

aagacaaata cggtataacc gaagcaccta acaccatgga caccgttctg gatgttgctt      180

cagaagtcac tttctacgga ctggaacagt acgaagaata cccatcacta cttgaagatc      240

agttcggagg atcacagaga gcagcagtaa ccgctgcagc atctgcatgt tccactggat      300

ttgcaactgg aaacgcccaa actgctttaa gtggatggta tctctctatg tacctgcaca      360

aagaacagca cagccgactt ggattctacg gttacgacct tcaggaccag tgtggtgcat      420

ctaacgtatt ctcaattaga ggagatgaag gattaccact ggaattgaga ggagctaact      480

atccaaacta cgc                                                         493


<210>  7
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Forward primer for amplifying 16S rRNA gene of Archaeal 
       communities

<400>  7
ttccggttga tccygccgga                                                   20


<210>  8
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Reverse primer for amplifying 16S rRNA gene of Archael 
       communities

<400>  8
yccggcgttg amtccaatt                                                    19


