                         SEQUENCE LISTING

<110>  New England Biolabs, Inc.
       Zhelkovsky, Alexander
       McReynolds, Larry
 
<120>  Compositions and Methods for Efficiently Adenylating 
       Oligonucleotides

<130>  NEB-332-PCT

<150>  61/320,203
<151>  2010-04-01

<150>  61/427,179
<151>  2010-12-25

<150>  61/427,178
<151>  2010-12-25

<160>  14    

<170>  PatentIn version 3.4

<210>  1
<211>  395
<212>  PRT
<213>  Bacteriophage TS2126

<400>  1

Met Ser Ser Leu Ala Pro Trp Arg Thr Thr Ser Trp Ser Pro Leu Gly 
1               5                   10                  15      


Ser Pro Pro Ser Leu Glu Asp Ala Leu Arg Leu Ala Arg Thr Thr Arg 
            20                  25                  30          


Ala Phe Ala Val Arg Arg Asp Gly Glu Gly Arg Ala Leu Val Thr Tyr 
        35                  40                  45              


Leu Tyr Gly Thr Pro Glu Leu Phe Ser Leu Pro Gly Ala Arg Glu Leu 
    50                  55                  60                  


Arg Gly Ile Val Tyr Arg Glu Glu Asp Gly Thr Val Leu Ser Arg Pro 
65                  70                  75                  80  


Phe His Lys Phe Phe Asn Phe Gly Glu Pro Leu Ala Pro Gly Glu Glu 
                85                  90                  95      


Ala Phe Lys Ala Phe Arg Asp Ser Met Val Pro Leu Phe Val Ala Glu 
            100                 105                 110         


Lys Val Asp Gly Tyr Leu Ala Gln Ala Tyr Leu Asp Gly Gly Glu Leu 
        115                 120                 125             


Arg Phe Ala Ser Arg His Ser Leu Asn Pro Pro Leu Val Gly Ala Leu 
    130                 135                 140                 


Leu Arg Lys Ala Val Asp Glu Glu Ala Met Ala Arg Leu Gly Lys Leu 
145                 150                 155                 160 


Leu Ala Ala Glu Gly Gly Arg Trp Thr Ala Leu Leu Glu Val Val Asp 
                165                 170                 175     


Pro Glu Ala Pro Val Met Val Pro Tyr Gln Glu Pro Gly Val Tyr Leu 
            180                 185                 190         


Leu Ala Leu Arg Ser Ile Gly Glu Gly His Tyr Leu Leu Pro Gly Val 
        195                 200                 205             


His Phe Pro Leu Pro Glu Ala Leu Arg Tyr Val Arg Trp Glu Pro Arg 
    210                 215                 220                 


Met Asp Phe Asp Pro His Arg Phe Arg Gly Glu Ile Arg Asp Leu Gln 
225                 230                 235                 240 


Gly Val Glu Gly Tyr Val Val Thr Asp Gly Ala Glu Phe Val Lys Phe 
                245                 250                 255     


Lys Thr Gly Trp Ala Phe Arg Leu Ala Arg Phe Leu Met Asp Pro Glu 
            260                 265                 270         


Gly Val Phe Leu Glu Ala Tyr Ala Glu Asp Arg Leu Asp Asp Leu Val 
        275                 280                 285             


Gly Ala Leu Ala Gly Arg Glu Asp Leu Leu Arg Ala Val Ala Arg Ala 
    290                 295                 300                 


Gln Asp Tyr Leu Ala Gly Leu Tyr Gly Glu Ala Val Gly Ala Gly Asp 
305                 310                 315                 320 


Ala Leu Arg Arg Met Gly Leu Pro Arg Lys Glu Ala Trp Ala Arg Val 
                325                 330                 335     


Gln Glu Glu Ala Gly Arg Trp Gly Gly Phe Ala Pro Ala Tyr Ala Arg 
            340                 345                 350         


Ala Ala Met Ala Ala Tyr Glu Gly Gly Glu Ala Arg Glu Ala Phe Leu 
        355                 360                 365             


Val Glu Leu Arg Lys Arg Ser Ala Arg Lys Ala Leu Glu Ala Leu His 
    370                 375                 380                 


Leu Phe Pro Arg Val Gly Gly Glu Leu Arg Gly 
385                 390                 395 


<210>  2
<211>  380
<212>  PRT
<213>  Methanobacterium thermoautotrophicum

<400>  2

Met Asn Ser Met Asn Ser Asp Ile Pro Phe Asp Leu Ile Gln Glu Arg 
1               5                   10                  15      


Thr Gly Val Pro Ser Ser Arg Leu Lys Val Ala Phe Ala Arg Gly Ser 
            20                  25                  30          


Leu Arg Leu Leu Glu Ser Ala Gly Met Gln Ala Leu Leu Phe Lys Lys 
        35                  40                  45              


Pro Leu Gly Asp Leu Glu Ala Gly Thr Val Ile Tyr Leu Gly Asp Glu 
    50                  55                  60                  


Thr Glu Val Ile Arg Gly Phe Pro Lys Ile Arg Arg Thr Leu Leu Leu 
65                  70                  75                  80  


Ser Pro Thr Ile Gln Glu His Phe Arg Asp Arg Val Ala Val Glu Glu 
                85                  90                  95      


Lys Met Asn Gly Tyr Asn Val Arg Ile Ala Cys Leu Ser Ser Gly Glu 
            100                 105                 110         


Thr Val Ala Leu Thr Arg Gly Gly His Val Cys Pro Phe Thr Thr Arg 
        115                 120                 125             


Lys Ala Gln Glu Leu Leu Asp Leu Ser Glu Phe Phe Arg Glu His Pro 
    130                 135                 140                 


Asp Leu Val Ile Cys Gly Glu Met Ile Gly Arg Asp Asn Pro Tyr Val 
145                 150                 155                 160 


Ser Gln Asp Tyr Pro Glu Val Gly Pro Leu Gly Phe Arg Val Phe Asp 
                165                 170                 175     


Leu Arg Glu Lys Asn Thr Asn Arg Pro Leu Pro Val Glu Glu Arg Arg 
            180                 185                 190         


Ala Leu Leu Asp Ser Tyr Gly Leu Pro Asn Val Arg Leu Phe Gly Val 
        195                 200                 205             


Tyr Pro Ile Glu Glu Ala Ala Ser Glu Val Ala Asp Ile Ile Arg Ala 
    210                 215                 220                 


Leu Gly Met Ala Gly Arg Glu Gly Val Val Met Lys Asp Pro Ser Met 
225                 230                 235                 240 


Glu Val Pro Pro Leu Lys Tyr Ser Ser Gln Ala His Ala Arg Glu Leu 
                245                 250                 255     


Ala Tyr Ala Phe Ser Tyr Pro Phe Asp Phe Gly Arg Pro Phe Phe Phe 
            260                 265                 270         


Ser Arg Val Ile Arg Glu Gly Phe Gln Ala Tyr Glu Leu Asp Glu Ser 
        275                 280                 285             


Asp Asp Glu Thr Arg Glu Arg Ala Arg Arg Leu Gly Glu Ala Ile Ile 
    290                 295                 300                 


Tyr Pro Met Leu Glu Arg Ile Lys Ser Ile Ser Ala Gly Glu Ala Ala 
305                 310                 315                 320 


Tyr Glu Asp Thr Val Ile Asp Val Glu Asp Arg Glu Ala Ala Glu Glu 
                325                 330                 335     


Phe Ile Arg His Leu Val Arg Leu Gly Val Ser Ala Thr Leu Ala Asp 
            340                 345                 350         


Tyr Arg Asp Gly Arg Ala Thr Ile Arg Arg Phe Tyr Gln Ser Thr Thr 
        355                 360                 365             


Asp Arg Ile Asn Asn Tyr Leu Lys Gly Gly Leu Tyr 
    370                 375                 380 


<210>  3
<211>  50
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide

<400>  3
agtgaattcg agctcggtac ccggtggatc ctctagagtc gacctgcagg                50


<210>  4
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide

<400>  4
tcgtatgccg tcttctgctt g                                               21


<210>  5
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide

<400>  5
tcgtatgccg tcttctgctt g                                               21


<210>  6
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide

<400>  6
ctatagaaac ccacgcaaag ccc                                             23


<210>  7
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide

<400>  7
ctgtaggcac catcaat                                                    17


<210>  8
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide

<400>  8
atgtaggcac catcaat                                                    17


<210>  9
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide

<400>  9
ttgtaggcac catcaat                                                    17


<210>  10
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide

<400>  10
gtgtaggcac catcaat                                                    17


<210>  11
<211>  23
<212>  RNA
<213>  artificial

<220>
<223>  acceptor

<400>  11
cugaugaaac ccacgcaaag ccc                                             23


<210>  12
<211>  22
<212>  RNA
<213>  artificial

<220>
<223>  acceptor

<400>  12
cuauacaacc uacuaccuca aa                                              22


<210>  13
<211>  26
<212>  RNA
<213>  artificial 

<220>
<223>  oligonucleotide

<400>  13
guucagaguu cuacaguccg acgauc                                          26


<210>  14
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide

<400>  14
tcgtatgccg tcttctgctt gt                                              22


