                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  THERMOSTABLE PHYTASE VARIANTS

<130>  11540-WO-PCT

<150>  EP 10158031.4
<151>  2010-03-26

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  1239
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1239)

<400>  1
cag agt gag ccg gag ctg aag ctg gaa agt gtg gtg att gtc agt cgt         48
Gln Ser Glu Pro Glu Leu Lys Leu Glu Ser Val Val Ile Val Ser Arg           
1               5                   10                  15                

cat ggt gtg cgt gct cca acc aag gcc acg caa ctg atg cag gat gtc         96
His Gly Val Arg Ala Pro Thr Lys Ala Thr Gln Leu Met Gln Asp Val           
            20                  25                  30                    

acc cca gac gca tgg cca acc tgg ccg gta aaa ctg ggt tgg ctg aca        144
Thr Pro Asp Ala Trp Pro Thr Trp Pro Val Lys Leu Gly Trp Leu Thr           
        35                  40                  45                        

ccg cgc ggt ggt gag cta atc gcc tat ctc gga cat tac caa cgc cag        192
Pro Arg Gly Gly Glu Leu Ile Ala Tyr Leu Gly His Tyr Gln Arg Gln           
    50                  55                  60                            

cgt ctg gta gcc gac gga ttg ctg gcg aaa aag ggc tgc ccg cag tct        240
Arg Leu Val Ala Asp Gly Leu Leu Ala Lys Lys Gly Cys Pro Gln Ser           
65                  70                  75                  80            

ggt cag gtc gcg att att gct gat gtc gac gag cgt acc cgt aaa aca        288
Gly Gln Val Ala Ile Ile Ala Asp Val Asp Glu Arg Thr Arg Lys Thr           
                85                  90                  95                

ggc gaa gcc ttc gcc gcc ggg ctg gca cct gac tgt gca ata acc gta        336
Gly Glu Ala Phe Ala Ala Gly Leu Ala Pro Asp Cys Ala Ile Thr Val           
            100                 105                 110                   

cat acc cag aca gat acg tcc agt ccc gat ccg tta ttt aat cct cta        384
His Thr Gln Thr Asp Thr Ser Ser Pro Asp Pro Leu Phe Asn Pro Leu           
        115                 120                 125                       

aaa act ggc gtt tgc caa ctg gat aac gcg aac gtg act gac gcg atc        432
Lys Thr Gly Val Cys Gln Leu Asp Asn Ala Asn Val Thr Asp Ala Ile           
    130                 135                 140                           

ctc agc agg gca gga ggg tca att gct gac ttt acc ggg cat cgg caa        480
Leu Ser Arg Ala Gly Gly Ser Ile Ala Asp Phe Thr Gly His Arg Gln           
145                 150                 155                 160           

acg gcg ttt cgc gaa ctg gaa cgg gtg ctt aat ttt ccg caa tca aac        528
Thr Ala Phe Arg Glu Leu Glu Arg Val Leu Asn Phe Pro Gln Ser Asn           
                165                 170                 175               

ttg tgc ctt aaa cgt gag aaa cag gac gaa agc tgt tca tta acg cag        576
Leu Cys Leu Lys Arg Glu Lys Gln Asp Glu Ser Cys Ser Leu Thr Gln           
            180                 185                 190                   

gca tta cca tcg gaa ctc aag gtg agc gcc gac aat gtc tca tta acc        624
Ala Leu Pro Ser Glu Leu Lys Val Ser Ala Asp Asn Val Ser Leu Thr           
        195                 200                 205                       

ggt gcg gta agc ctc gca tca atg ctg acg gag ata ttt ctc ctg caa        672
Gly Ala Val Ser Leu Ala Ser Met Leu Thr Glu Ile Phe Leu Leu Gln           
    210                 215                 220                           

caa gca cag gga atg ccg gag ccg ggg tgg gga agg atc acc gat tca        720
Gln Ala Gln Gly Met Pro Glu Pro Gly Trp Gly Arg Ile Thr Asp Ser           
225                 230                 235                 240           

cac cag tgg aac acc ttg cta agt ttg cat aac gcg caa ttt tat ttg        768
His Gln Trp Asn Thr Leu Leu Ser Leu His Asn Ala Gln Phe Tyr Leu           
                245                 250                 255               

cta caa cgc acg cca gag gtt gcc cgc agc cgc gcc acc ccg tta tta        816
Leu Gln Arg Thr Pro Glu Val Ala Arg Ser Arg Ala Thr Pro Leu Leu           
            260                 265                 270                   

gat ttg atc aag aca gcg ttg acg ccc cat cca ccg caa aaa cag gcg        864
Asp Leu Ile Lys Thr Ala Leu Thr Pro His Pro Pro Gln Lys Gln Ala           
        275                 280                 285                       

tat ggt gtg aca tta ccc act tca gtg ctg ttt atc gcc gga cac gat        912
Tyr Gly Val Thr Leu Pro Thr Ser Val Leu Phe Ile Ala Gly His Asp           
    290                 295                 300                           

act aat ctg gca aat ctc ggc ggc gca ctg gag ctc aac tgg acg ctt        960
Thr Asn Leu Ala Asn Leu Gly Gly Ala Leu Glu Leu Asn Trp Thr Leu           
305                 310                 315                 320           

ccc ggt cag ccg gat aac acg ccg cca ggt ggt gaa ctg gtg ttt gaa       1008
Pro Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Glu Leu Val Phe Glu           
                325                 330                 335               

cgc tgg cgt cgg cta agc gat aac agc cag tgg att cag gtt tcg ctg       1056
Arg Trp Arg Arg Leu Ser Asp Asn Ser Gln Trp Ile Gln Val Ser Leu           
            340                 345                 350                   

gtc ttc cag act tta cag cag atg cgt gat aaa acg ccg ctg tca tta       1104
Val Phe Gln Thr Leu Gln Gln Met Arg Asp Lys Thr Pro Leu Ser Leu           
        355                 360                 365                       

aat acg ccg ccc gga gag gtg aaa ctg acc ctg gca gga tgt gaa gag       1152
Asn Thr Pro Pro Gly Glu Val Lys Leu Thr Leu Ala Gly Cys Glu Glu           
    370                 375                 380                           

cga aat gcg cag ggc atg tgt tcg ttg gca ggt ttt acg caa atc gtg       1200
Arg Asn Ala Gln Gly Met Cys Ser Leu Ala Gly Phe Thr Gln Ile Val           
385                 390                 395                 400           

aat gaa gca cgc ata ccg gcg tgc agt ttg cgt agt taa                   1239
Asn Glu Ala Arg Ile Pro Ala Cys Ser Leu Arg Ser                           
                405                 410                                   


<210>  2
<211>  412
<212>  PRT
<213>  Escherichia coli

<400>  2

Gln Ser Glu Pro Glu Leu Lys Leu Glu Ser Val Val Ile Val Ser Arg 
1               5                   10                  15      


His Gly Val Arg Ala Pro Thr Lys Ala Thr Gln Leu Met Gln Asp Val 
            20                  25                  30          


Thr Pro Asp Ala Trp Pro Thr Trp Pro Val Lys Leu Gly Trp Leu Thr 
        35                  40                  45              


Pro Arg Gly Gly Glu Leu Ile Ala Tyr Leu Gly His Tyr Gln Arg Gln 
    50                  55                  60                  


Arg Leu Val Ala Asp Gly Leu Leu Ala Lys Lys Gly Cys Pro Gln Ser 
65                  70                  75                  80  


Gly Gln Val Ala Ile Ile Ala Asp Val Asp Glu Arg Thr Arg Lys Thr 
                85                  90                  95      


Gly Glu Ala Phe Ala Ala Gly Leu Ala Pro Asp Cys Ala Ile Thr Val 
            100                 105                 110         


His Thr Gln Thr Asp Thr Ser Ser Pro Asp Pro Leu Phe Asn Pro Leu 
        115                 120                 125             


Lys Thr Gly Val Cys Gln Leu Asp Asn Ala Asn Val Thr Asp Ala Ile 
    130                 135                 140                 


Leu Ser Arg Ala Gly Gly Ser Ile Ala Asp Phe Thr Gly His Arg Gln 
145                 150                 155                 160 


Thr Ala Phe Arg Glu Leu Glu Arg Val Leu Asn Phe Pro Gln Ser Asn 
                165                 170                 175     


Leu Cys Leu Lys Arg Glu Lys Gln Asp Glu Ser Cys Ser Leu Thr Gln 
            180                 185                 190         


Ala Leu Pro Ser Glu Leu Lys Val Ser Ala Asp Asn Val Ser Leu Thr 
        195                 200                 205             


Gly Ala Val Ser Leu Ala Ser Met Leu Thr Glu Ile Phe Leu Leu Gln 
    210                 215                 220                 


Gln Ala Gln Gly Met Pro Glu Pro Gly Trp Gly Arg Ile Thr Asp Ser 
225                 230                 235                 240 


His Gln Trp Asn Thr Leu Leu Ser Leu His Asn Ala Gln Phe Tyr Leu 
                245                 250                 255     


Leu Gln Arg Thr Pro Glu Val Ala Arg Ser Arg Ala Thr Pro Leu Leu 
            260                 265                 270         


Asp Leu Ile Lys Thr Ala Leu Thr Pro His Pro Pro Gln Lys Gln Ala 
        275                 280                 285             


Tyr Gly Val Thr Leu Pro Thr Ser Val Leu Phe Ile Ala Gly His Asp 
    290                 295                 300                 


Thr Asn Leu Ala Asn Leu Gly Gly Ala Leu Glu Leu Asn Trp Thr Leu 
305                 310                 315                 320 


Pro Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Glu Leu Val Phe Glu 
                325                 330                 335     


Arg Trp Arg Arg Leu Ser Asp Asn Ser Gln Trp Ile Gln Val Ser Leu 
            340                 345                 350         


Val Phe Gln Thr Leu Gln Gln Met Arg Asp Lys Thr Pro Leu Ser Leu 
        355                 360                 365             


Asn Thr Pro Pro Gly Glu Val Lys Leu Thr Leu Ala Gly Cys Glu Glu 
    370                 375                 380                 


Arg Asn Ala Gln Gly Met Cys Ser Leu Ala Gly Phe Thr Gln Ile Val 
385                 390                 395                 400 


Asn Glu Ala Arg Ile Pro Ala Cys Ser Leu Arg Ser 
                405                 410         


<210>  3
<211>  412
<212>  PRT
<213>  Escherichia coli


<220>
<221>  mat_peptide
<222>  (1)..(412)

<400>  3

Met Ala Gln Ser Glu Pro Glu Leu Lys Leu Glu Ser Val Val Ile Val 
1               5                   10                  15      


Ser Arg His Gly Val Arg Ala Pro Thr Lys Ala Thr Gln Leu Met Gln 
            20                  25                  30          


Asp Val Thr Pro Asp Ala Trp Pro Thr Trp Pro Val Lys Leu Gly Trp 
        35                  40                  45              


Leu Thr Pro Arg Gly Gly Glu Leu Ile Ala Tyr Leu Gly His Tyr Gln 
    50                  55                  60                  


Arg Gln Arg Leu Val Ala Asp Gly Leu Leu Ala Lys Lys Gly Cys Pro 
65                  70                  75                  80  


Gln Ser Gly Gln Val Ala Ile Ile Ala Asp Val Asp Glu Arg Thr Arg 
                85                  90                  95      


Lys Thr Gly Glu Ala Phe Ala Ala Gly Leu Ala Pro Asp Cys Ala Ile 
            100                 105                 110         


Thr Val His Thr Gln Ala Asp Thr Ser Ser Pro Asp Pro Leu Phe Asn 
        115                 120                 125             


Pro Leu Lys Thr Gly Val Cys Gln Leu Asp Asn Ala Asn Val Thr Asp 
    130                 135                 140                 


Ala Ile Leu Ser Arg Ala Gly Gly Ser Ile Ala Asp Phe Thr Gly His 
145                 150                 155                 160 


Arg Gln Thr Ala Phe Arg Glu Leu Glu Arg Val Leu Asn Phe Pro Gln 
                165                 170                 175     


Ser Asn Leu Cys Leu Lys Arg Glu Lys Gln Asp Glu Ser Cys Ser Leu 
            180                 185                 190         


Thr Gln Ala Leu Pro Ser Glu Leu Lys Val Ser Ala Asp Asn Val Ser 
        195                 200                 205             


Leu Thr Gly Ala Val Ser Leu Ala Ser Met Leu Thr Glu Ile Phe Leu 
    210                 215                 220                 


Leu Gln Gln Ala Gln Gly Met Pro Glu Pro Gly Trp Gly Arg Ile Thr 
225                 230                 235                 240 


Asp Ser His Gln Trp Asn Thr Leu Leu Ser Leu His Asn Ala Gln Phe 
                245                 250                 255     


Tyr Leu Leu Gln Arg Thr Pro Glu Val Ala Arg Ser Arg Ala Thr Pro 
            260                 265                 270         


Leu Leu Asp Leu Ile Lys Thr Ala Leu Thr Pro His Pro Pro Gln Lys 
        275                 280                 285             


Gln Ala Tyr Gly Val Thr Leu Pro Thr Ser Val Leu Phe Ile Ala Gly 
    290                 295                 300                 


His Asp Thr Asn Leu Ala Asn Leu Gly Gly Ala Leu Glu Leu Asn Trp 
305                 310                 315                 320 


Thr Leu Pro Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Glu Leu Val 
                325                 330                 335     


Phe Glu Arg Trp Arg Arg Leu Ser Asp Asn Ser Gln Trp Ile Gln Val 
            340                 345                 350         


Ser Leu Val Phe Gln Thr Leu Gln Gln Met Arg Asp Lys Thr Pro Leu 
        355                 360                 365             


Ser Leu Asn Thr Pro Pro Gly Glu Val Lys Leu Thr Leu Ala Gly Cys 
    370                 375                 380                 


Glu Glu Arg Asn Ala Gln Gly Met Cys Ser Leu Ala Gly Phe Thr Gln 
385                 390                 395                 400 


Ile Val Asn Glu Ala Arg Ile Pro Ala Cys Ser Leu 
                405                 410         


<210>  4
<211>  412
<212>  PRT
<213>  Escherichia coli


<220>
<221>  VARIANT
<222>  (1)..(412)
<223>  Variant Nov9X from WO 2009/073399

<400>  4

Met Ala Gln Ser Glu Pro Glu Leu Lys Leu Glu Ser Val Val Ile Val 
1               5                   10                  15      


Ser Arg His Gly Val Arg Ala Pro Thr Lys Ala Thr Gln Leu Met Gln 
            20                  25                  30          


Asp Val Thr Pro Asp Ala Trp Pro Thr Trp Pro Val Lys Leu Gly Glu 
        35                  40                  45              


Leu Thr Pro Arg Gly Gly Glu Leu Ile Ala Tyr Leu Gly His Tyr Trp 
    50                  55                  60                  


Arg Gln Arg Leu Val Ala Asp Gly Leu Leu Pro Lys Cys Gly Cys Pro 
65                  70                  75                  80  


Gln Ser Gly Gln Val Ala Ile Ile Ala Asp Val Asp Glu Arg Thr Arg 
                85                  90                  95      


Lys Thr Gly Glu Ala Phe Ala Ala Gly Leu Ala Pro Asp Cys Ala Ile 
            100                 105                 110         


Thr Val His Thr Gln Ala Asp Thr Ser Ser Pro Asp Pro Leu Phe Asn 
        115                 120                 125             


Pro Leu Lys Thr Gly Val Cys Gln Leu Asp Asn Ala Asn Val Thr Asp 
    130                 135                 140                 


Ala Ile Leu Glu Arg Ala Gly Gly Ser Ile Ala Asp Phe Thr Gly His 
145                 150                 155                 160 


Tyr Gln Thr Ala Phe Arg Glu Leu Glu Arg Val Leu Asn Phe Pro Gln 
                165                 170                 175     


Ser Asn Leu Cys Leu Lys Arg Glu Lys Gln Asp Glu Ser Cys Ser Leu 
            180                 185                 190         


Thr Gln Ala Leu Pro Ser Glu Leu Lys Val Ser Ala Asp Cys Val Ser 
        195                 200                 205             


Leu Thr Gly Ala Val Ser Leu Ala Ser Met Leu Thr Glu Ile Phe Leu 
    210                 215                 220                 


Leu Gln Gln Ala Gln Gly Met Pro Glu Pro Gly Trp Gly Arg Ile Thr 
225                 230                 235                 240 


Asp Ser His Gln Trp Asn Thr Leu Leu Ser Leu His Asn Ala Gln Phe 
                245                 250                 255     


Asp Leu Leu Gln Arg Thr Pro Glu Val Ala Arg Ser Arg Ala Thr Pro 
            260                 265                 270         


Leu Leu Asp Leu Ile Lys Thr Ala Leu Thr Pro His Pro Pro Gln Lys 
        275                 280                 285             


Gln Ala Tyr Gly Val Thr Leu Pro Thr Ser Val Leu Phe Ile Ala Gly 
    290                 295                 300                 


His Asp Thr Asn Leu Ala Asn Leu Gly Gly Ala Leu Glu Leu Asn Trp 
305                 310                 315                 320 


Thr Leu Pro Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Glu Leu Val 
                325                 330                 335     


Phe Glu Arg Trp Arg Arg Leu Ser Asp Asn Ser Gln Trp Ile Gln Val 
            340                 345                 350         


Ser Leu Val Phe Gln Thr Leu Gln Gln Met Arg Asp Lys Thr Pro Leu 
        355                 360                 365             


Ser Leu Asn Thr Pro Pro Gly Glu Val Lys Leu Thr Leu Ala Gly Cys 
    370                 375                 380                 


Glu Glu Arg Asn Ala Gln Gly Met Cys Ser Leu Ala Gly Phe Thr Gln 
385                 390                 395                 400 


Ile Val Asn Glu Ala Arg Ile Pro Ala Cys Ser Leu 
                405                 410         


<210>  5
<211>  432
<212>  PRT
<213>  Escherichia coli


<220>
<221>  PROPEP
<222>  (1)..(22)

<220>
<221>  mat_peptide
<222>  (23)..(432)

<400>  5

Met Lys Ala Ile Leu Ile Pro Phe Leu Ser Leu Leu Ile Pro Leu Thr 
        -20                 -15                 -10             


Pro Gln Ser Ala Phe Ala Gln Ser Glu Pro Glu Leu Lys Leu Glu Ser 
    -5              -1  1               5                   10  


Val Val Ile Val Ser Arg His Gly Val Arg Ala Pro Thr Lys Ala Thr 
                15                  20                  25      


Gln Leu Met Gln Asp Val Thr Pro Asp Ala Trp Pro Thr Trp Pro Val 
            30                  35                  40          


Lys Leu Gly Glu Leu Thr Pro Arg Gly Gly Glu Leu Ile Ala Tyr Leu 
        45                  50                  55              


Gly His Tyr Trp Arg Gln Arg Leu Val Ala Asp Gly Leu Leu Pro Lys 
    60                  65                  70                  


Cys Gly Cys Pro Gln Ser Gly Gln Val Ala Ile Ile Ala Asp Val Asp 
75                  80                  85                  90  


Glu Arg Thr Arg Lys Thr Gly Glu Ala Phe Ala Ala Gly Leu Ala Pro 
                95                  100                 105     


Asp Cys Ala Ile Thr Val His Thr Gln Ala Asp Thr Ser Ser Pro Asp 
            110                 115                 120         


Pro Leu Phe Asn Pro Leu Lys Thr Gly Val Cys Gln Leu Asp Asn Ala 
        125                 130                 135             


Asn Val Thr Asp Ala Ile Leu Glu Arg Ala Gly Gly Ser Ile Ala Asp 
    140                 145                 150                 


Phe Thr Gly His Tyr Gln Thr Ala Phe Arg Glu Leu Glu Arg Val Leu 
155                 160                 165                 170 


Asn Phe Pro Gln Ser Asn Leu Cys Leu Lys Arg Glu Lys Gln Asp Glu 
                175                 180                 185     


Ser Cys Ser Leu Thr Gln Ala Leu Pro Ser Glu Leu Lys Val Ser Ala 
            190                 195                 200         


Asp Cys Val Ser Leu Thr Gly Ala Val Ser Leu Ala Ser Met Leu Thr 
        205                 210                 215             


Glu Ile Phe Leu Leu Gln Gln Ala Gln Gly Met Pro Glu Pro Gly Trp 
    220                 225                 230                 


Gly Arg Ile Thr Asp Ser His Gln Trp Asn Thr Leu Leu Ser Leu His 
235                 240                 245                 250 


Asn Ala Gln Phe Asp Leu Leu Gln Arg Thr Pro Glu Val Ala Arg Ser 
                255                 260                 265     


Arg Ala Thr Pro Leu Leu Asp Leu Ile Lys Thr Ala Leu Thr Pro His 
            270                 275                 280         


Pro Pro Gln Lys Gln Ala Tyr Gly Val Thr Leu Pro Thr Ser Val Leu 
        285                 290                 295             


Phe Ile Ala Gly His Asp Thr Asn Leu Ala Asn Leu Gly Gly Ala Leu 
    300                 305                 310                 


Glu Leu Asn Trp Thr Leu Pro Gly Gln Pro Asp Asn Thr Pro Pro Gly 
315                 320                 325                 330 


Gly Glu Leu Val Phe Glu Arg Trp Arg Arg Leu Ser Asp Asn Ser Gln 
                335                 340                 345     


Trp Ile Gln Val Ser Leu Val Phe Gln Thr Leu Gln Gln Met Arg Asp 
            350                 355                 360         


Lys Thr Pro Leu Ser Leu Asn Thr Pro Pro Gly Glu Val Lys Leu Thr 
        365                 370                 375             


Leu Ala Gly Cys Glu Glu Arg Asn Ala Gln Gly Met Cys Ser Leu Ala 
    380                 385                 390                 


Gly Phe Thr Gln Ile Val Asn Glu Ala Arg Ile Pro Ala Cys Ser Leu 
395                 400                 405                 410 


<210>  6
<211>  432
<212>  PRT
<213>  Escherichia coli


<220>
<221>  PROPEP
<222>  (1)..(22)

<220>
<221>  mat_peptide
<222>  (23)..(432)

<400>  6

Met Lys Ala Ile Leu Ile Pro Phe Leu Ser Leu Leu Ile Pro Leu Thr 
        -20                 -15                 -10             


Pro Gln Ser Ala Phe Ala Gln Ser Glu Pro Glu Leu Lys Leu Glu Ser 
    -5              -1  1               5                   10  


Val Val Ile Val Ser Arg His Gly Val Arg Ala Pro Thr Lys Ala Thr 
                15                  20                  25      


Gln Leu Met Gln Asp Val Thr Pro Asp Ala Trp Pro Thr Trp Pro Val 
            30                  35                  40          


Lys Leu Gly Trp Leu Thr Pro Arg Gly Gly Glu Leu Ile Ala Tyr Leu 
        45                  50                  55              


Gly His Tyr Gln Arg Gln Arg Leu Val Ala Asp Gly Leu Leu Ala Lys 
    60                  65                  70                  


Lys Gly Cys Pro Gln Ser Gly Gln Val Ala Ile Ile Ala Asp Val Asp 
75                  80                  85                  90  


Glu Arg Thr Arg Lys Thr Gly Glu Ala Phe Ala Ala Gly Leu Ala Pro 
                95                  100                 105     


Asp Cys Ala Ile Thr Val His Thr Gln Ala Asp Thr Ser Ser Pro Asp 
            110                 115                 120         


Pro Leu Phe Asn Pro Leu Lys Thr Gly Val Cys Gln Leu Asp Asn Ala 
        125                 130                 135             


Asn Val Thr Asp Ala Ile Leu Ser Arg Ala Gly Gly Ser Ile Ala Asp 
    140                 145                 150                 


Phe Thr Gly His Arg Gln Thr Ala Phe Arg Glu Leu Glu Arg Val Leu 
155                 160                 165                 170 


Asn Phe Pro Gln Ser Asn Leu Cys Leu Lys Arg Glu Lys Gln Asp Glu 
                175                 180                 185     


Ser Cys Ser Leu Thr Gln Ala Leu Pro Ser Glu Leu Lys Val Ser Ala 
            190                 195                 200         


Asp Asn Val Ser Leu Thr Gly Ala Val Ser Leu Ala Ser Met Leu Thr 
        205                 210                 215             


Glu Ile Phe Leu Leu Gln Gln Ala Gln Gly Met Pro Glu Pro Gly Trp 
    220                 225                 230                 


Gly Arg Ile Thr Asp Ser His Gln Trp Asn Thr Leu Leu Ser Leu His 
235                 240                 245                 250 


Asn Ala Gln Phe Tyr Leu Leu Gln Arg Thr Pro Glu Val Ala Arg Ser 
                255                 260                 265     


Arg Ala Thr Pro Leu Leu Asp Leu Ile Lys Thr Ala Leu Thr Pro His 
            270                 275                 280         


Pro Pro Gln Lys Gln Ala Tyr Gly Val Thr Leu Pro Thr Ser Val Leu 
        285                 290                 295             


Phe Ile Ala Gly His Asp Thr Asn Leu Ala Asn Leu Gly Gly Ala Leu 
    300                 305                 310                 


Glu Leu Asn Trp Thr Leu Pro Gly Gln Pro Asp Asn Thr Pro Pro Gly 
315                 320                 325                 330 


Gly Glu Leu Val Phe Glu Arg Trp Arg Arg Leu Ser Asp Asn Ser Gln 
                335                 340                 345     


Trp Ile Gln Val Ser Leu Val Phe Gln Thr Leu Gln Gln Met Arg Asp 
            350                 355                 360         


Lys Thr Pro Leu Ser Leu Asn Thr Pro Pro Gly Glu Val Lys Leu Thr 
        365                 370                 375             


Leu Ala Gly Cys Glu Glu Arg Asn Ala Gln Gly Met Cys Ser Leu Ala 
    380                 385                 390                 


Gly Phe Thr Gln Ile Val Asn Glu Ala Arg Ile Pro Ala Cys Ser Leu 
395                 400                 405                 410 


