                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  Phytase variants

<130>  11523-WO-PCT

<160>  9     

<170>  PatentIn version 3.4

<210>  1
<211>  1233
<212>  DNA
<213>  Citrobacter braakii ATCC 51113


<220>
<221>  mat_peptide
<222>  (1)..(1233)

<220>
<221>  CDS
<222>  (1)..(1233)

<400>  1
gaa gag cag aat ggt atg aaa ctt gag cgg gtt gtg ata gtg agt cgt       48
Glu Glu Gln Asn Gly Met Lys Leu Glu Arg Val Val Ile Val Ser Arg         
1               5                   10                  15              

cat gga gta aga gca cct acg aag ttc act cca ata atg aaa aat gtc       96
His Gly Val Arg Ala Pro Thr Lys Phe Thr Pro Ile Met Lys Asn Val         
            20                  25                  30                  

aca ccc gat caa tgg cca caa tgg gat gtg ccg tta gga tgg cta acg      144
Thr Pro Asp Gln Trp Pro Gln Trp Asp Val Pro Leu Gly Trp Leu Thr         
        35                  40                  45                      

cct cgt ggg gga gaa ctt gtt tct gaa tta ggt cag tat caa cgt tta      192
Pro Arg Gly Gly Glu Leu Val Ser Glu Leu Gly Gln Tyr Gln Arg Leu         
    50                  55                  60                          

tgg ttc acg agc aaa ggt ctg ttg aat aat caa acg tgc cca tct cca      240
Trp Phe Thr Ser Lys Gly Leu Leu Asn Asn Gln Thr Cys Pro Ser Pro         
65                  70                  75                  80          

ggg cag gtt gct gtt att gca gac acg gat caa cgc acc cgt aaa acg      288
Gly Gln Val Ala Val Ile Ala Asp Thr Asp Gln Arg Thr Arg Lys Thr         
                85                  90                  95              

ggt gag gcg ttt ctg gct ggg tta gca cca aaa tgt caa att caa gtg      336
Gly Glu Ala Phe Leu Ala Gly Leu Ala Pro Lys Cys Gln Ile Gln Val         
            100                 105                 110                 

cat tat cag aag gat gaa gaa aaa aat gat cct ctt ttt aat ccg gta      384
His Tyr Gln Lys Asp Glu Glu Lys Asn Asp Pro Leu Phe Asn Pro Val         
        115                 120                 125                     

aaa atg ggg aaa tgt tcg ttt aac aca ttg cag gtt aaa aac gct att      432
Lys Met Gly Lys Cys Ser Phe Asn Thr Leu Gln Val Lys Asn Ala Ile         
    130                 135                 140                         

ctg gaa cgg gcc gga gga aat att gaa ctg tat acc caa cgc tat caa      480
Leu Glu Arg Ala Gly Gly Asn Ile Glu Leu Tyr Thr Gln Arg Tyr Gln         
145                 150                 155                 160         

tct tca ttt cgg acc ctg gaa aat gtt tta aat ttc tca caa tcg gag      528
Ser Ser Phe Arg Thr Leu Glu Asn Val Leu Asn Phe Ser Gln Ser Glu         
                165                 170                 175             

aca tgt aag act aca gaa aag tct acg aaa tgc aca tta cca gag gct      576
Thr Cys Lys Thr Thr Glu Lys Ser Thr Lys Cys Thr Leu Pro Glu Ala         
            180                 185                 190                 

tta ccg tct gaa ctt aag gta act cct gac aat gta tca tta cct ggt      624
Leu Pro Ser Glu Leu Lys Val Thr Pro Asp Asn Val Ser Leu Pro Gly         
        195                 200                 205                     

gcc tgg agt ctt tct tcc acg ctg act gag ata ttt ctg ttg caa gag      672
Ala Trp Ser Leu Ser Ser Thr Leu Thr Glu Ile Phe Leu Leu Gln Glu         
    210                 215                 220                         

gcc cag gga atg cca cag gta gcc tgg ggg cgt att acg gga gaa aaa      720
Ala Gln Gly Met Pro Gln Val Ala Trp Gly Arg Ile Thr Gly Glu Lys         
225                 230                 235                 240         

gaa tgg aga gat ttg tta agt ctg cat aac gct cag ttt gat ctt ttg      768
Glu Trp Arg Asp Leu Leu Ser Leu His Asn Ala Gln Phe Asp Leu Leu         
                245                 250                 255             

caa aga act cca gaa gtt gcc cgt agt agg gcc aca cca tta ctc gat      816
Gln Arg Thr Pro Glu Val Ala Arg Ser Arg Ala Thr Pro Leu Leu Asp         
            260                 265                 270                 

atg ata gac act gca tta ttg aca aat ggt aca aca gaa aac agg tat      864
Met Ile Asp Thr Ala Leu Leu Thr Asn Gly Thr Thr Glu Asn Arg Tyr         
        275                 280                 285                     

ggc ata aaa tta ccc gta tct ctg ttg ttt att gct ggt cat gat acc      912
Gly Ile Lys Leu Pro Val Ser Leu Leu Phe Ile Ala Gly His Asp Thr         
    290                 295                 300                         

aat ctt gca aat tta agc ggg gct tta gat ctt aac tgg tcg cta ccc      960
Asn Leu Ala Asn Leu Ser Gly Ala Leu Asp Leu Asn Trp Ser Leu Pro         
305                 310                 315                 320         

ggt caa ccc gat aat acc cct cct ggt ggg gag ctt gta ttc gaa aag     1008
Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Glu Leu Val Phe Glu Lys         
                325                 330                 335             

tgg aaa aga acc agt gat aat acg gat tgg gtt cag gtt tca ttt gtt     1056
Trp Lys Arg Thr Ser Asp Asn Thr Asp Trp Val Gln Val Ser Phe Val         
            340                 345                 350                 

tat cag acg ctg aga gat atg agg gat ata caa ccg ttg tcg tta gaa     1104
Tyr Gln Thr Leu Arg Asp Met Arg Asp Ile Gln Pro Leu Ser Leu Glu         
        355                 360                 365                     

aaa cct gct ggc aaa gtt gat tta aaa tta att gca tgt gaa gag aaa     1152
Lys Pro Ala Gly Lys Val Asp Leu Lys Leu Ile Ala Cys Glu Glu Lys         
    370                 375                 380                         

aat agt cag gga atg tgt tcg tta aaa agt ttt tcc agg ctc att aag     1200
Asn Ser Gln Gly Met Cys Ser Leu Lys Ser Phe Ser Arg Leu Ile Lys         
385                 390                 395                 400         

gaa att cgc gtg cca gag tgt gca gtt acg gaa                         1233
Glu Ile Arg Val Pro Glu Cys Ala Val Thr Glu                             
                405                 410                                 


<210>  2
<211>  411
<212>  PRT
<213>  Citrobacter braakii ATCC 51113

<400>  2

Glu Glu Gln Asn Gly Met Lys Leu Glu Arg Val Val Ile Val Ser Arg 
1               5                   10                  15      


His Gly Val Arg Ala Pro Thr Lys Phe Thr Pro Ile Met Lys Asn Val 
            20                  25                  30          


Thr Pro Asp Gln Trp Pro Gln Trp Asp Val Pro Leu Gly Trp Leu Thr 
        35                  40                  45              


Pro Arg Gly Gly Glu Leu Val Ser Glu Leu Gly Gln Tyr Gln Arg Leu 
    50                  55                  60                  


Trp Phe Thr Ser Lys Gly Leu Leu Asn Asn Gln Thr Cys Pro Ser Pro 
65                  70                  75                  80  


Gly Gln Val Ala Val Ile Ala Asp Thr Asp Gln Arg Thr Arg Lys Thr 
                85                  90                  95      


Gly Glu Ala Phe Leu Ala Gly Leu Ala Pro Lys Cys Gln Ile Gln Val 
            100                 105                 110         


His Tyr Gln Lys Asp Glu Glu Lys Asn Asp Pro Leu Phe Asn Pro Val 
        115                 120                 125             


Lys Met Gly Lys Cys Ser Phe Asn Thr Leu Gln Val Lys Asn Ala Ile 
    130                 135                 140                 


Leu Glu Arg Ala Gly Gly Asn Ile Glu Leu Tyr Thr Gln Arg Tyr Gln 
145                 150                 155                 160 


Ser Ser Phe Arg Thr Leu Glu Asn Val Leu Asn Phe Ser Gln Ser Glu 
                165                 170                 175     


Thr Cys Lys Thr Thr Glu Lys Ser Thr Lys Cys Thr Leu Pro Glu Ala 
            180                 185                 190         


Leu Pro Ser Glu Leu Lys Val Thr Pro Asp Asn Val Ser Leu Pro Gly 
        195                 200                 205             


Ala Trp Ser Leu Ser Ser Thr Leu Thr Glu Ile Phe Leu Leu Gln Glu 
    210                 215                 220                 


Ala Gln Gly Met Pro Gln Val Ala Trp Gly Arg Ile Thr Gly Glu Lys 
225                 230                 235                 240 


Glu Trp Arg Asp Leu Leu Ser Leu His Asn Ala Gln Phe Asp Leu Leu 
                245                 250                 255     


Gln Arg Thr Pro Glu Val Ala Arg Ser Arg Ala Thr Pro Leu Leu Asp 
            260                 265                 270         


Met Ile Asp Thr Ala Leu Leu Thr Asn Gly Thr Thr Glu Asn Arg Tyr 
        275                 280                 285             


Gly Ile Lys Leu Pro Val Ser Leu Leu Phe Ile Ala Gly His Asp Thr 
    290                 295                 300                 


Asn Leu Ala Asn Leu Ser Gly Ala Leu Asp Leu Asn Trp Ser Leu Pro 
305                 310                 315                 320 


Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Glu Leu Val Phe Glu Lys 
                325                 330                 335     


Trp Lys Arg Thr Ser Asp Asn Thr Asp Trp Val Gln Val Ser Phe Val 
            340                 345                 350         


Tyr Gln Thr Leu Arg Asp Met Arg Asp Ile Gln Pro Leu Ser Leu Glu 
        355                 360                 365             


Lys Pro Ala Gly Lys Val Asp Leu Lys Leu Ile Ala Cys Glu Glu Lys 
    370                 375                 380                 


Asn Ser Gln Gly Met Cys Ser Leu Lys Ser Phe Ser Arg Leu Ile Lys 
385                 390                 395                 400 


Glu Ile Arg Val Pro Glu Cys Ala Val Thr Glu 
                405                 410     


<210>  3
<211>  411
<212>  PRT
<213>  Citrobacter braakii YH-15


<220>
<221>  mat_peptide
<222>  (1)..(411)

<400>  3

Glu Glu Gln Asn Gly Met Lys Leu Glu Arg Val Val Ile Val Ser Arg 
1               5                   10                  15      


His Gly Val Arg Ala Pro Thr Lys Phe Thr Pro Ile Met Lys Asp Val 
            20                  25                  30          


Thr Pro Asp Gln Trp Pro Gln Trp Asp Val Pro Leu Gly Trp Leu Thr 
        35                  40                  45              


Pro Arg Gly Gly Glu Leu Val Ser Glu Leu Gly Gln Tyr Gln Arg Leu 
    50                  55                  60                  


Trp Phe Thr Ser Lys Gly Leu Leu Asn Asn Gln Thr Cys Pro Ser Pro 
65                  70                  75                  80  


Gly Gln Val Ala Val Ile Ala Asp Thr Asp Gln Arg Thr Arg Lys Thr 
                85                  90                  95      


Gly Glu Ala Phe Leu Ala Gly Leu Ala Pro Lys Cys Gln Ile Gln Val 
            100                 105                 110         


His Tyr Gln Lys Asp Glu Glu Lys Asn Asp Pro Leu Phe Asn Pro Val 
        115                 120                 125             


Lys Met Gly Lys Cys Ser Phe Asn Thr Leu Lys Val Lys Asn Ala Ile 
    130                 135                 140                 


Leu Glu Arg Ala Gly Gly Asn Ile Glu Leu Tyr Thr Gln Arg Tyr Gln 
145                 150                 155                 160 


Ser Ser Phe Arg Thr Leu Glu Asn Val Leu Asn Phe Ser Gln Ser Glu 
                165                 170                 175     


Thr Cys Lys Thr Thr Glu Lys Ser Thr Lys Cys Thr Leu Pro Glu Ala 
            180                 185                 190         


Leu Pro Ser Glu Phe Lys Val Thr Pro Asp Asn Val Ser Leu Pro Gly 
        195                 200                 205             


Ala Trp Ser Leu Ser Ser Thr Leu Thr Glu Ile Phe Leu Leu Gln Glu 
    210                 215                 220                 


Ala Gln Gly Met Pro Gln Val Ala Trp Gly Arg Ile Thr Gly Glu Lys 
225                 230                 235                 240 


Glu Trp Arg Asp Leu Leu Ser Leu His Asn Ala Gln Phe Asp Leu Leu 
                245                 250                 255     


Gln Arg Thr Pro Glu Val Ala Arg Ser Arg Ala Thr Pro Leu Leu Asp 
            260                 265                 270         


Met Ile Asp Thr Ala Leu Leu Thr Asn Gly Thr Thr Glu Asn Arg Tyr 
        275                 280                 285             


Gly Ile Lys Leu Pro Val Ser Leu Leu Phe Ile Ala Gly His Asp Thr 
    290                 295                 300                 


Asn Leu Ala Asn Leu Ser Gly Ala Leu Asp Leu Lys Trp Ser Leu Pro 
305                 310                 315                 320 


Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Glu Leu Val Phe Glu Lys 
                325                 330                 335     


Trp Lys Arg Thr Ser Asp Asn Thr Asp Trp Val Gln Val Ser Phe Val 
            340                 345                 350         


Tyr Gln Thr Leu Arg Asp Met Arg Asp Ile Gln Pro Leu Ser Leu Glu 
        355                 360                 365             


Lys Pro Ala Gly Lys Val Asp Leu Lys Leu Ile Ala Cys Glu Glu Lys 
    370                 375                 380                 


Asn Ser Gln Gly Met Cys Ser Leu Lys Ser Phe Ser Arg Leu Ile Lys 
385                 390                 395                 400 


Glu Ile Arg Val Pro Glu Cys Ala Val Thr Glu 
                405                 410     


<210>  4
<211>  411
<212>  PRT
<213>  Citrobacter freundii


<220>
<221>  mat_peptide
<222>  (1)..(411)

<400>  4

Glu Glu Gln Asn Gly Met Lys Leu Glu Arg Val Val Ile Val Ser Arg 
1               5                   10                  15      


His Gly Val Arg Ala Pro Thr Lys Phe Thr Pro Ile Met Lys Asp Val 
            20                  25                  30          


Thr Pro Asp Gln Trp Pro Gln Trp Asp Val Pro Leu Gly Trp Leu Thr 
        35                  40                  45              


Pro Arg Gly Gly Glu Leu Val Ser Glu Leu Gly Gln Tyr Gln Arg Leu 
    50                  55                  60                  


Trp Phe Thr Ser Lys Gly Leu Leu Asn Asn Gln Thr Cys Pro Ser Pro 
65                  70                  75                  80  


Gly Gln Val Ala Val Ile Ala Asp Thr Asp Gln Arg Thr Arg Lys Thr 
                85                  90                  95      


Gly Glu Ala Phe Leu Ala Gly Leu Ala Pro Lys Cys Gln Ile Gln Val 
            100                 105                 110         


His Tyr Gln Lys Asp Glu Glu Lys Thr Asp Pro Leu Phe Asn Pro Val 
        115                 120                 125             


Lys Met Gly Thr Cys Ser Phe Asn Thr Leu Lys Val Lys Asn Ala Ile 
    130                 135                 140                 


Leu Glu Arg Ala Gly Gly Asn Ile Glu Leu Tyr Thr Gln Arg Tyr Gln 
145                 150                 155                 160 


Ser Ser Phe Arg Thr Leu Glu Asn Val Leu Asn Phe Ser Gln Ser Glu 
                165                 170                 175     


Thr Cys Lys Thr Thr Glu Lys Ser Thr Lys Cys Thr Leu Pro Glu Ala 
            180                 185                 190         


Leu Pro Ser Glu Leu Lys Val Thr Pro Asp Asn Val Ser Leu Pro Gly 
        195                 200                 205             


Ala Trp Ser Leu Ser Ser Thr Leu Thr Glu Ile Phe Leu Leu Gln Glu 
    210                 215                 220                 


Ala Gln Gly Met Pro Gln Val Ala Trp Gly Arg Ile Thr Gly Glu Lys 
225                 230                 235                 240 


Glu Trp Arg Asp Leu Leu Ser Leu His Asn Ala Gln Phe Asp Leu Leu 
                245                 250                 255     


Gln Arg Thr Pro Glu Val Ala Arg Ser Arg Ala Thr Pro Leu Leu Asp 
            260                 265                 270         


Met Ile Asp Thr Ala Leu Leu Thr Asn Gly Thr Thr Glu Asn Arg Tyr 
        275                 280                 285             


Gly Ile Lys Leu Pro Val Ser Leu Leu Phe Ile Ala Gly His Asp Thr 
    290                 295                 300                 


Asn Leu Ala Asn Leu Ser Gly Ala Leu Asp Leu Asn Trp Ser Leu Pro 
305                 310                 315                 320 


Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Glu Leu Val Phe Glu Lys 
                325                 330                 335     


Trp Lys Arg Thr Ser Asp Asn Thr Asp Trp Val Gln Val Ser Phe Val 
            340                 345                 350         


Tyr Gln Thr Leu Arg Asp Met Arg Asp Ile Gln Pro Leu Ser Leu Glu 
        355                 360                 365             


Lys Pro Ala Gly Lys Val Asp Leu Lys Leu Ile Ala Cys Glu Glu Lys 
    370                 375                 380                 


Asn Ser Gln Gly Met Cys Ser Leu Lys Ser Phe Ser Arg Leu Ile Lys 
385                 390                 395                 400 


Glu Ile Arg Val Pro Glu Cys Ala Val Thr Glu 
                405                 410     


<210>  5
<211>  1236
<212>  DNA
<213>  Variant of SEQ ID NO:2


<220>
<221>  CDS
<222>  (1)..(1233)

<220>
<221>  mat_peptide
<222>  (1)..(1233)

<400>  5
gaa gag cag aat ggt atg aaa ctt gag cgg gtt gtg ata gtg agt cgt       48
Glu Glu Gln Asn Gly Met Lys Leu Glu Arg Val Val Ile Val Ser Arg         
1               5                   10                  15              

cat ggr gta aga gca cct acg aag ttc act cca ata atg aaa aat gtc       96
His Xaa Val Arg Ala Pro Thr Lys Phe Thr Pro Ile Met Lys Asn Val         
            20                  25                  30                  

aca ccc gat caa tgg cca caa tgg gat gtg ccg tta gga tgg cta acg      144
Thr Pro Asp Gln Trp Pro Gln Trp Asp Val Pro Leu Gly Trp Leu Thr         
        35                  40                  45                      

cct cgt ggg gga gaa ctt gtt tct gaa tta ggt cag tat caa cgt tta      192
Pro Arg Gly Gly Glu Leu Val Ser Glu Leu Gly Gln Tyr Gln Arg Leu         
    50                  55                  60                          

tgg ttc acg agc aaa ggt ctg ttg aat aat caa acg tgc cca tct cca      240
Trp Phe Thr Ser Lys Gly Leu Leu Asn Asn Gln Thr Cys Pro Ser Pro         
65                  70                  75                  80          

ggg cag gtt gct gtt att gca gac acg gat caa cgc acc cgt aaa acg      288
Gly Gln Val Ala Val Ile Ala Asp Thr Asp Gln Arg Thr Arg Lys Thr         
                85                  90                  95              

ggt gag gcg ttt ctg gct ggg tta gca cca aaa tgt caa att caa gtg      336
Gly Glu Ala Phe Leu Ala Gly Leu Ala Pro Lys Cys Gln Ile Gln Val         
            100                 105                 110                 

cat tat cag aag gat gaa gaa aaa aat gat cct ctt ttt aat ccg gta      384
His Tyr Gln Lys Asp Glu Glu Lys Asn Asp Pro Leu Phe Asn Pro Val         
        115                 120                 125                     

aaa atg ggg aaa tgt tcg ttt aac aca ttg cag gtt aaa aac gct att      432
Lys Met Gly Lys Cys Ser Phe Asn Thr Leu Gln Val Lys Asn Ala Ile         
    130                 135                 140                         

ctg gaa cgg gcc gga gga aat att gaa ctg tat acc caa cgc tat caa      480
Leu Glu Arg Ala Gly Gly Asn Ile Glu Leu Tyr Thr Gln Arg Tyr Gln         
145                 150                 155                 160         

tct tca ttt cgg acc ctg gaa aat gtt tta aat ttc tca caa tcg gag      528
Ser Ser Phe Arg Thr Leu Glu Asn Val Leu Asn Phe Ser Gln Ser Glu         
                165                 170                 175             

aca tgt aag act aca gaa aag tct acg aaa tgc aca tta cca gag gct      576
Thr Cys Lys Thr Thr Glu Lys Ser Thr Lys Cys Thr Leu Pro Glu Ala         
            180                 185                 190                 

tta ccg tct gaa ctt aag gta act cct gac aat gta tca tta cct ggt      624
Leu Pro Ser Glu Leu Lys Val Thr Pro Asp Asn Val Ser Leu Pro Gly         
        195                 200                 205                     

gcc tgg agt ctt tct tcc acg ctg act gag ata ttt ctg ttg caa gag      672
Ala Trp Ser Leu Ser Ser Thr Leu Thr Glu Ile Phe Leu Leu Gln Glu         
    210                 215                 220                         

gcc cag gga atg cca cag gta gcc tgg ggg cgt att acg gga gaa aaa      720
Ala Gln Gly Met Pro Gln Val Ala Trp Gly Arg Ile Thr Gly Glu Lys         
225                 230                 235                 240         

gaa tgg aga gat ttg tta agt ctg cat aac gct cag ttt gat ctt ttg      768
Glu Trp Arg Asp Leu Leu Ser Leu His Asn Ala Gln Phe Asp Leu Leu         
                245                 250                 255             

caa aga act cca gaa gtt gcc cgt agt agg gcc aca cca tta ctc gat      816
Gln Arg Thr Pro Glu Val Ala Arg Ser Arg Ala Thr Pro Leu Leu Asp         
            260                 265                 270                 

atg ata gac act gca tta ttg aca aat ggt aca aca gaa aac agg tat      864
Met Ile Asp Thr Ala Leu Leu Thr Asn Gly Thr Thr Glu Asn Arg Tyr         
        275                 280                 285                     

ggc ata aaa tta ccc gta tct ctg ttg ttt att gct ggt cat gat acc      912
Gly Ile Lys Leu Pro Val Ser Leu Leu Phe Ile Ala Gly His Asp Thr         
    290                 295                 300                         

aat ctt gca aat tta agc ggg gct tta gat ctt aac tgg tcg cta ccc      960
Asn Leu Ala Asn Leu Ser Gly Ala Leu Asp Leu Asn Trp Ser Leu Pro         
305                 310                 315                 320         

ggt caa ccs gat aay acc ccg ccg ggc gac aag ctt gta ttc gaa aag     1008
Gly Gln Xaa Asp Asn Thr Pro Pro Gly Asp Lys Leu Val Phe Glu Lys         
                325                 330                 335             

tgg aaa aga acc agt gat aat acg gat tgg gtt cag gtt tca ttt gtt     1056
Trp Lys Arg Thr Ser Asp Asn Thr Asp Trp Val Gln Val Ser Phe Val         
            340                 345                 350                 

tat cag acg ctg aga gat atg agg gat ata caa ccg ttg tcg tta gaa     1104
Tyr Gln Thr Leu Arg Asp Met Arg Asp Ile Gln Pro Leu Ser Leu Glu         
        355                 360                 365                     

aaa cct gct ggc aaa gtt gat tta aaa tta att gca tgt gaa gag aaa     1152
Lys Pro Ala Gly Lys Val Asp Leu Lys Leu Ile Ala Cys Glu Glu Lys         
    370                 375                 380                         

aat agt cag gga atg tgt tcg tta aaa agt ttt tcc agg ctc att aag     1200
Asn Ser Gln Gly Met Cys Ser Leu Lys Ser Phe Ser Arg Leu Ile Lys         
385                 390                 395                 400         

gaa att cgc gtg cca gag tgt gca gtt acg gaa taa                     1236
Glu Ile Arg Val Pro Glu Cys Ala Val Thr Glu                             
                405                 410                                 


<210>  6
<211>  411
<212>  PRT
<213>  Variant of SEQ ID NO:2

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  The 'Xaa' at location 18 stands for Gly.

<220>
<221>  misc_feature
<222>  (323)..(323)
<223>  The 'Xaa' at location 323 stands for Pro.

<400>  6

Glu Glu Gln Asn Gly Met Lys Leu Glu Arg Val Val Ile Val Ser Arg 
1               5                   10                  15      


His Xaa Val Arg Ala Pro Thr Lys Phe Thr Pro Ile Met Lys Asn Val 
            20                  25                  30          


Thr Pro Asp Gln Trp Pro Gln Trp Asp Val Pro Leu Gly Trp Leu Thr 
        35                  40                  45              


Pro Arg Gly Gly Glu Leu Val Ser Glu Leu Gly Gln Tyr Gln Arg Leu 
    50                  55                  60                  


Trp Phe Thr Ser Lys Gly Leu Leu Asn Asn Gln Thr Cys Pro Ser Pro 
65                  70                  75                  80  


Gly Gln Val Ala Val Ile Ala Asp Thr Asp Gln Arg Thr Arg Lys Thr 
                85                  90                  95      


Gly Glu Ala Phe Leu Ala Gly Leu Ala Pro Lys Cys Gln Ile Gln Val 
            100                 105                 110         


His Tyr Gln Lys Asp Glu Glu Lys Asn Asp Pro Leu Phe Asn Pro Val 
        115                 120                 125             


Lys Met Gly Lys Cys Ser Phe Asn Thr Leu Gln Val Lys Asn Ala Ile 
    130                 135                 140                 


Leu Glu Arg Ala Gly Gly Asn Ile Glu Leu Tyr Thr Gln Arg Tyr Gln 
145                 150                 155                 160 


Ser Ser Phe Arg Thr Leu Glu Asn Val Leu Asn Phe Ser Gln Ser Glu 
                165                 170                 175     


Thr Cys Lys Thr Thr Glu Lys Ser Thr Lys Cys Thr Leu Pro Glu Ala 
            180                 185                 190         


Leu Pro Ser Glu Leu Lys Val Thr Pro Asp Asn Val Ser Leu Pro Gly 
        195                 200                 205             


Ala Trp Ser Leu Ser Ser Thr Leu Thr Glu Ile Phe Leu Leu Gln Glu 
    210                 215                 220                 


Ala Gln Gly Met Pro Gln Val Ala Trp Gly Arg Ile Thr Gly Glu Lys 
225                 230                 235                 240 


Glu Trp Arg Asp Leu Leu Ser Leu His Asn Ala Gln Phe Asp Leu Leu 
                245                 250                 255     


Gln Arg Thr Pro Glu Val Ala Arg Ser Arg Ala Thr Pro Leu Leu Asp 
            260                 265                 270         


Met Ile Asp Thr Ala Leu Leu Thr Asn Gly Thr Thr Glu Asn Arg Tyr 
        275                 280                 285             


Gly Ile Lys Leu Pro Val Ser Leu Leu Phe Ile Ala Gly His Asp Thr 
    290                 295                 300                 


Asn Leu Ala Asn Leu Ser Gly Ala Leu Asp Leu Asn Trp Ser Leu Pro 
305                 310                 315                 320 


Gly Gln Xaa Asp Asn Thr Pro Pro Gly Asp Lys Leu Val Phe Glu Lys 
                325                 330                 335     


Trp Lys Arg Thr Ser Asp Asn Thr Asp Trp Val Gln Val Ser Phe Val 
            340                 345                 350         


Tyr Gln Thr Leu Arg Asp Met Arg Asp Ile Gln Pro Leu Ser Leu Glu 
        355                 360                 365             


Lys Pro Ala Gly Lys Val Asp Leu Lys Leu Ile Ala Cys Glu Glu Lys 
    370                 375                 380                 


Asn Ser Gln Gly Met Cys Ser Leu Lys Ser Phe Ser Arg Leu Ile Lys 
385                 390                 395                 400 


Glu Ile Arg Val Pro Glu Cys Ala Val Thr Glu 
                405                 410     


<210>  7
<211>  66
<212>  DNA
<213>  Citrobacter braakii ATCC 51113


<220>
<221>  sig_peptide
<222>  (1)..(66)

<220>
<221>  CDS
<222>  (1)..(66)

<400>  7
atg agt aca ttc atc att cgt tta tta ttt ttt tct ctc tta tgc ggt       48
Met Ser Thr Phe Ile Ile Arg Leu Leu Phe Phe Ser Leu Leu Cys Gly         
1               5                   10                  15              

tct ttc tca ata cat gct                                               66
Ser Phe Ser Ile His Ala                                                 
            20                                                          


<210>  8
<211>  22
<212>  PRT
<213>  Citrobacter braakii ATCC 51113

<400>  8

Met Ser Thr Phe Ile Ile Arg Leu Leu Phe Phe Ser Leu Leu Cys Gly 
1               5                   10                  15      


Ser Phe Ser Ile His Ala 
            20          


<210>  9
<211>  433
<212>  PRT
<213>  Citrobacter freundii NCIMB 41247


<220>
<221>  mat_peptide
<222>  (1)..(433)

<400>  9

Met Ser Thr Phe Ile Ile Arg Leu Leu Phe Phe Ser Leu Leu Cys Gly 
1               5                   10                  15      


Ser Phe Ser Ile His Ala Glu Glu Pro Asn Gly Met Lys Leu Glu Arg 
            20                  25                  30          


Val Val Ile Val Ser Arg His Gly Val Arg Ala Pro Thr Lys Phe Thr 
        35                  40                  45              


Pro Ile Met Lys Asp Val Thr Pro Asp Gln Trp Pro Gln Trp Asp Val 
    50                  55                  60                  


Pro Leu Gly Trp Leu Thr Pro Arg Gly Gly Glu Leu Val Ser Glu Leu 
65                  70                  75                  80  


Gly Gln Tyr Gln Arg Leu Trp Phe Thr Ser Lys Gly Leu Leu Asn Asn 
                85                  90                  95      


Gln Thr Cys Pro Ser Pro Gly Gln Val Ala Val Ile Ala Asp Thr Asp 
            100                 105                 110         


Gln Arg Thr Arg Lys Thr Gly Glu Ala Phe Leu Ala Gly Leu Ala Pro 
        115                 120                 125             


Lys Cys Gln Ile Gln Val His Tyr Gln Lys Asp Glu Glu Lys Thr Asp 
    130                 135                 140                 


Pro Leu Phe Asn Pro Val Lys Met Gly Thr Cys Ser Phe Asn Thr Leu 
145                 150                 155                 160 


Lys Val Lys Asn Ala Ile Leu Glu Arg Ala Gly Gly Asn Ile Glu Leu 
                165                 170                 175     


Tyr Thr Gln Arg Tyr Gln Ser Ser Phe Arg Thr Leu Glu Asn Val Leu 
            180                 185                 190         


Asn Phe Ser Gln Ser Glu Thr Cys Lys Thr Thr Glu Lys Ser Thr Lys 
        195                 200                 205             


Cys Thr Leu Pro Glu Ala Leu Pro Ser Glu Leu Lys Val Thr Pro Asp 
    210                 215                 220                 


Asn Val Ser Leu Pro Gly Ala Trp Ser Leu Ser Ser Thr Leu Thr Glu 
225                 230                 235                 240 


Ile Phe Leu Leu Gln Glu Ala Gln Gly Met Pro Gln Val Ala Trp Gly 
                245                 250                 255     


Arg Ile Thr Gly Glu Lys Glu Trp Arg Asp Leu Leu Ser Leu His Asn 
            260                 265                 270         


Ala Gln Phe Asp Leu Leu Gln Arg Thr Pro Glu Val Ala Arg Ser Arg 
        275                 280                 285             


Ala Thr Pro Leu Leu Asp Met Ile Asp Thr Ala Leu Leu Thr Asn Gly 
    290                 295                 300                 


Thr Thr Glu Asn Arg Tyr Gly Ile Lys Leu Pro Val Ser Leu Leu Phe 
305                 310                 315                 320 


Ile Ala Gly His Asp Thr Asn Leu Ala Asn Leu Ser Gly Ala Leu Asp 
                325                 330                 335     


Leu Asn Trp Ser Leu Pro Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly 
            340                 345                 350         


Glu Leu Val Phe Glu Lys Trp Lys Arg Thr Ser Asp Asn Thr Asp Trp 
        355                 360                 365             


Val Gln Val Ser Phe Val Tyr Gln Thr Leu Arg Asp Met Arg Asp Ile 
    370                 375                 380                 


Gln Pro Leu Ser Leu Glu Lys Pro Ala Gly Lys Val Asp Leu Lys Leu 
385                 390                 395                 400 


Ile Ala Cys Glu Glu Lys Asn Ser Gln Gly Met Cys Ser Leu Lys Ser 
                405                 410                 415     


Phe Ser Arg Leu Ile Lys Glu Ile Arg Val Pro Glu Cys Ala Val Thr 
            420                 425                 430         


Glu 
    


