                         SEQUENCE LISTING

<110>  Amsterdam Molecular Therapeutics (AMT) IP B.V. 
       Noordman, Yvet
       Lubelski, Jacek
       Bakker, Andrew Christian
 
<120>  Mutated Rep encoding sequences for use in AAV production

<130>  P6034703PCT

<150>  61/312,845
<151>  2010-11-03

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  1866
<212>  DNA
<213>  adeno-associated virus 2


<220>
<221>  CDS
<222>  (1)..(1866)

<400>  1
atg ccg ggg ttt tac gag att gtg att aag gtc ccc agc gac ctt gac         48
Met Pro Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp           
1               5                   10                  15                

gag cat ctg ccc ggc att tct gac agc ttt gtg aac tgg gtg gcc gag         96
Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu           
            20                  25                  30                    

aag gaa tgg gag ttg ccg cca gat tct gac atg gat ctg aat ctg att        144
Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile           
        35                  40                  45                        

gag cag gca ccc ctg acc gtg gcc gag aag ctg cag cgc gac ttt ctg        192
Glu Gln Ala Pro Leu Thr Val Ala Glu Lys Leu Gln Arg Asp Phe Leu           
    50                  55                  60                            

acg gaa tgg cgc cgt gtg agt aag gcc ccg gag gcc ctt ttc ttt gtg        240
Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Phe Val           
65                  70                  75                  80            

caa ttt gag aag gga gag agc tac ttc cac atg cac gtg ctc gtg gaa        288
Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu           
                85                  90                  95                

acc acc ggg gtg aaa tcc atg gtt ttg gga cgt ttc ctg agt cag att        336
Thr Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile           
            100                 105                 110                   

cgc gaa aaa ctg att cag aga att tac cgc ggg atc gag ccg act ttg        384
Arg Glu Lys Leu Ile Gln Arg Ile Tyr Arg Gly Ile Glu Pro Thr Leu           
        115                 120                 125                       

cca aac tgg ttc gcg gtc aca aag acc aga aat ggc gcc gga ggc ggg        432
Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly           
    130                 135                 140                           

aac aag gtg gtg gat gag tgc tac atc ccc aat tac ttg ctc ccc aaa        480
Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys           
145                 150                 155                 160           

acc cag cct gag ctc cag tgg gcg tgg act aat atg gaa cag tat tta        528
Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu           
                165                 170                 175               

agc gcc tgt ttg aat ctc acg gag cgt aaa cgg ttg gtg gcg cag cat        576
Ser Ala Cys Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His           
            180                 185                 190                   

ctg acg cac gtg tcg cag acg cag gag cag aac aaa gag aat cag aat        624
Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn           
        195                 200                 205                       

ccc aat tct gat gcg ccg gtg atc aga tca aaa act tca gcc agg tac        672
Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr           
    210                 215                 220                           

atg gag ctg gtc ggg tgg ctc gtg gac aag ggg att acc tcg gag aag        720
Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys           
225                 230                 235                 240           

cag tgg atc cag gag gac cag gcc tca tac atc tcc ttc aat gcg gcc        768
Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala           
                245                 250                 255               

tcc aac tcg cgg tcc caa atc aag gct gcc ttg gac aat gcg gga aag        816
Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys           
            260                 265                 270                   

att atg agc ctg act aaa acc gcc ccc gac tac ctg gtg ggc cag cag        864
Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln           
        275                 280                 285                       

ccc gtg gag gac att tcc agc aat cgg att tat aaa att ttg gaa cta        912
Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu           
    290                 295                 300                           

aac ggg tac gat ccc caa tat gcg gct tcc gtc ttt ctg gga tgg gcc        960
Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala           
305                 310                 315                 320           

acg aaa aag ttc ggc aag agg aac acc atc tgg ctg ttt ggg cct gca       1008
Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala           
                325                 330                 335               

act acc ggg aag acc aac atc gcg gag gcc ata gcc cac act gtg ccc       1056
Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro           
            340                 345                 350                   

ttc tac ggg tgc gta aac tgg acc aat gag aac ttt ccc ttc aac gac       1104
Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp           
        355                 360                 365                       

tgt gtc gac aag atg gtg atc tgg tgg gag gag ggg aag atg acc gcc       1152
Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala           
    370                 375                 380                           

aag gtc gtg gag tcg gcc aaa gcc att ctc gga gga agc aag gtg cgc       1200
Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg           
385                 390                 395                 400           

gtg gac cag aaa tgc aag tcc tcg gcc cag ata gac ccg act ccc gtg       1248
Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val           
                405                 410                 415               

atc gtc acc tcc aac acc aac atg tgc gcc gtg att gac ggg aac tca       1296
Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser           
            420                 425                 430                   

acg acc ttc gaa cac cag cag ccg ttg caa gac cgg atg ttc aaa ttt       1344
Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe           
        435                 440                 445                       

gaa ctc acc cgc cgt ctg gat cat gac ttt ggg aag gtc acc aag cag       1392
Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln           
    450                 455                 460                           

gaa gtc aaa gac ttt ttc cgg tgg gca aag gat cac gtg gtt gag gtg       1440
Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val           
465                 470                 475                 480           

gag cat gaa ttc tac gtc aaa aag ggt gga gcc aag aaa aga ccc gcc       1488
Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys Lys Arg Pro Ala           
                485                 490                 495               

ccc agt gac gca gat ata agt gag ccc aaa cgg gtg cgc gag tca gtt       1536
Pro Ser Asp Ala Asp Ile Ser Glu Pro Lys Arg Val Arg Glu Ser Val           
            500                 505                 510                   

gcg cag cca tcg acg tca gac gcg gaa gct tcg atc aac tac gca gac       1584
Ala Gln Pro Ser Thr Ser Asp Ala Glu Ala Ser Ile Asn Tyr Ala Asp           
        515                 520                 525                       

agg tac caa aac aaa tgt tct cgt cac gtg ggc atg aat ctg atg ctg       1632
Arg Tyr Gln Asn Lys Cys Ser Arg His Val Gly Met Asn Leu Met Leu           
    530                 535                 540                           

ttt ccc tgc aga caa tgc gag aga atg aat cag aat tca aat atc tgc       1680
Phe Pro Cys Arg Gln Cys Glu Arg Met Asn Gln Asn Ser Asn Ile Cys           
545                 550                 555                 560           

ttc act cac gga cag aaa gac tgt tta gag tgc ttt ccc gtg tca gaa       1728
Phe Thr His Gly Gln Lys Asp Cys Leu Glu Cys Phe Pro Val Ser Glu           
                565                 570                 575               

tct caa ccc gtt tct gtc gtc aaa aag gcg tat cag aaa ctg tgc tac       1776
Ser Gln Pro Val Ser Val Val Lys Lys Ala Tyr Gln Lys Leu Cys Tyr           
            580                 585                 590                   

att cat cat atc atg gga aag gtg cca gac gct tgc act gcc tgc gat       1824
Ile His His Ile Met Gly Lys Val Pro Asp Ala Cys Thr Ala Cys Asp           
        595                 600                 605                       

ctg gtc aat gtg gat ttg gat gac tgc atc ttt gaa caa taa               1866
Leu Val Asn Val Asp Leu Asp Asp Cys Ile Phe Glu Gln                       
    610                 615                 620                           


<210>  2
<211>  621
<212>  PRT
<213>  adeno-associated virus 2

<400>  2

Met Pro Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp 
1               5                   10                  15      


Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu 
            20                  25                  30          


Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile 
        35                  40                  45              


Glu Gln Ala Pro Leu Thr Val Ala Glu Lys Leu Gln Arg Asp Phe Leu 
    50                  55                  60                  


Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Phe Val 
65                  70                  75                  80  


Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu 
                85                  90                  95      


Thr Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile 
            100                 105                 110         


Arg Glu Lys Leu Ile Gln Arg Ile Tyr Arg Gly Ile Glu Pro Thr Leu 
        115                 120                 125             


Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly 
    130                 135                 140                 


Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys 
145                 150                 155                 160 


Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu 
                165                 170                 175     


Ser Ala Cys Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His 
            180                 185                 190         


Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn 
        195                 200                 205             


Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr 
    210                 215                 220                 


Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys 
225                 230                 235                 240 


Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala 
                245                 250                 255     


Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys 
            260                 265                 270         


Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln 
        275                 280                 285             


Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu 
    290                 295                 300                 


Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala 
305                 310                 315                 320 


Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala 
                325                 330                 335     


Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro 
            340                 345                 350         


Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp 
        355                 360                 365             


Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala 
    370                 375                 380                 


Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg 
385                 390                 395                 400 


Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val 
                405                 410                 415     


Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser 
            420                 425                 430         


Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe 
        435                 440                 445             


Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln 
    450                 455                 460                 


Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val 
465                 470                 475                 480 


Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys Lys Arg Pro Ala 
                485                 490                 495     


Pro Ser Asp Ala Asp Ile Ser Glu Pro Lys Arg Val Arg Glu Ser Val 
            500                 505                 510         


Ala Gln Pro Ser Thr Ser Asp Ala Glu Ala Ser Ile Asn Tyr Ala Asp 
        515                 520                 525             


Arg Tyr Gln Asn Lys Cys Ser Arg His Val Gly Met Asn Leu Met Leu 
    530                 535                 540                 


Phe Pro Cys Arg Gln Cys Glu Arg Met Asn Gln Asn Ser Asn Ile Cys 
545                 550                 555                 560 


Phe Thr His Gly Gln Lys Asp Cys Leu Glu Cys Phe Pro Val Ser Glu 
                565                 570                 575     


Ser Gln Pro Val Ser Val Val Lys Lys Ala Tyr Gln Lys Leu Cys Tyr 
            580                 585                 590         


Ile His His Ile Met Gly Lys Val Pro Asp Ala Cys Thr Ala Cys Asp 
        595                 600                 605             


Leu Val Asn Val Asp Leu Asp Asp Cys Ile Phe Glu Gln 
    610                 615                 620     


<210>  3
<211>  1866
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pVD88 variant of Rep78 from AAV2


<220>
<221>  CDS
<222>  (1)..(1866)

<400>  3
acg gcg ggg ttc tac gag att gtg att aag gtc ccc agc gac ctt gac         48
Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp           
1               5                   10                  15                

gag cat ctg ccc ggc att tct gac agc ttt gtg aac tgg gtg gcc gag         96
Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu           
            20                  25                  30                    

aag gaa tgg gag ttg ccg cca gat tct gac atg gat ctg aat ctg att        144
Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile           
        35                  40                  45                        

gag cag gca ccc ctg acc gtg gcc gag aag ctg cag cgc gac ttt ctg        192
Glu Gln Ala Pro Leu Thr Val Ala Glu Lys Leu Gln Arg Asp Phe Leu           
    50                  55                  60                            

acg gaa tgg cgc cgt gtg agt aag gcc ccg gag gcc ctt ttc ttt gtg        240
Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Phe Val           
65                  70                  75                  80            

caa ttt gag aag gga gag agc tac ttc cac atg cac gtg ctc gtg gaa        288
Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu           
                85                  90                  95                

acc acc ggg gtg aaa tcc atg gtt ttg gga cgt ttc ctg agt cag att        336
Thr Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile           
            100                 105                 110                   

cgc gaa aaa ctg att cag aga att tac cgc ggg atc gag ccg act ttg        384
Arg Glu Lys Leu Ile Gln Arg Ile Tyr Arg Gly Ile Glu Pro Thr Leu           
        115                 120                 125                       

cca aac tgg ttc gcg gtc aca aag acc aga aat ggc gcc gga ggc ggg        432
Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly           
    130                 135                 140                           

aac aag gtg gtg gat gag tgc tac atc ccc aat tac ttg ctc ccc aaa        480
Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys           
145                 150                 155                 160           

acc cag cct gag ctc cag tgg gcg tgg act aat atg gaa cag tat tta        528
Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu           
                165                 170                 175               

agc gcc tgt ttg aat ctc acg gag cgt aaa cgg ttg gtg gcg cag cat        576
Ser Ala Cys Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His           
            180                 185                 190                   

ctg acg cac gtg tcg cag acg cag gag cag aac aaa gag aat cag aat        624
Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn           
        195                 200                 205                       

ccc aat tct gat gcg ccg gtg atc aga tca aaa act tca gcc agg tac        672
Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr           
    210                 215                 220                           

atg gag ctg gtc ggg tgg ctc gtg gac aag ggg att acc tcg gag aag        720
Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys           
225                 230                 235                 240           

cag tgg atc cag gag gac cag gcc tca tac atc tcc ttc aat gcg gcc        768
Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala           
                245                 250                 255               

tcc aac tcg cgg tcc caa atc aag gct gcc ttg gac aat gcg gga aag        816
Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys           
            260                 265                 270                   

att atg agc ctg act aaa acc gcc ccc gac tac ctg gtg ggc cag cag        864
Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln           
        275                 280                 285                       

ccc gtg gag gac att tcc agc aat cgg att tat aaa att ttg gaa cta        912
Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu           
    290                 295                 300                           

aac ggg tac gat ccc caa tat gcg gct tcc gtc ttt ctg gga tgg gcc        960
Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala           
305                 310                 315                 320           

acg aaa aag ttc ggc aag agg aac acc atc tgg ctg ttt ggg cct gca       1008
Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala           
                325                 330                 335               

act acc ggg aag acc aac atc gcg gag gcc ata gcc cac act gtg ccc       1056
Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro           
            340                 345                 350                   

ttc tac ggg tgc gta aac tgg acc aat gag aac ttt ccc ttc aac gac       1104
Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp           
        355                 360                 365                       

tgt gtc gac aag atg gtg atc tgg tgg gag gag ggg aag atg acc gcc       1152
Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala           
    370                 375                 380                           

aag gtc gtg gag tcg gcc aaa gcc att ctc gga gga agc aag gtg cgc       1200
Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg           
385                 390                 395                 400           

gtg gac cag aaa tgc aag tcc tcg gcc cag ata gac ccg act ccc gtg       1248
Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val           
                405                 410                 415               

atc gtc acc tcc aac acc aac atg tgc gcc gtg att gac ggg aac tca       1296
Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser           
            420                 425                 430                   

acg acc ttc gaa cac cag cag ccg ttg caa gac cgg atg ttc aaa ttt       1344
Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe           
        435                 440                 445                       

gaa ctc acc cgc cgt ctg gat cat gac ttt ggg aag gtc acc aag cag       1392
Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln           
    450                 455                 460                           

gaa gtc aaa gac ttt ttc cgg tgg gca aag gat cac gtg gtt gag gtg       1440
Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val           
465                 470                 475                 480           

gag cat gaa ttc tac gtc aaa aag ggt gga gcc aag aaa aga ccc gcc       1488
Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys Lys Arg Pro Ala           
                485                 490                 495               

ccc agt gac gca gat ata agt gag ccc aaa cgg gtg cgc gag tca gtt       1536
Pro Ser Asp Ala Asp Ile Ser Glu Pro Lys Arg Val Arg Glu Ser Val           
            500                 505                 510                   

gcg cag cca tcg acg tca gac gcg gaa gct tcg atc aac tac gca gac       1584
Ala Gln Pro Ser Thr Ser Asp Ala Glu Ala Ser Ile Asn Tyr Ala Asp           
        515                 520                 525                       

agg tac caa aac aaa tgt tct cgt cac gtg ggc atg aat ctg atg ctg       1632
Arg Tyr Gln Asn Lys Cys Ser Arg His Val Gly Met Asn Leu Met Leu           
    530                 535                 540                           

ttt ccc tgc aga caa tgc gag aga atg aat cag aat tca aat atc tgc       1680
Phe Pro Cys Arg Gln Cys Glu Arg Met Asn Gln Asn Ser Asn Ile Cys           
545                 550                 555                 560           

ttc act cac gga cag aaa gac tgt tta gag tgc ttt ccc gtg tca gaa       1728
Phe Thr His Gly Gln Lys Asp Cys Leu Glu Cys Phe Pro Val Ser Glu           
                565                 570                 575               

tct caa ccc gtt tct gtc gtc aaa aag gcg tat cag aaa ctg tgc tac       1776
Ser Gln Pro Val Ser Val Val Lys Lys Ala Tyr Gln Lys Leu Cys Tyr           
            580                 585                 590                   

att cat cat atc atg gga aag gtg cca gac gct tgc act gcc tgc gat       1824
Ile His His Ile Met Gly Lys Val Pro Asp Ala Cys Thr Ala Cys Asp           
        595                 600                 605                       

ctg gtc aat gtg gat ttg gat gac tgc atc ttt gaa caa taa               1866
Leu Val Asn Val Asp Leu Asp Asp Cys Ile Phe Glu Gln                       
    610                 615                 620                           


<210>  4
<211>  621
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  4

Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp 
1               5                   10                  15      


Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu 
            20                  25                  30          


Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile 
        35                  40                  45              


Glu Gln Ala Pro Leu Thr Val Ala Glu Lys Leu Gln Arg Asp Phe Leu 
    50                  55                  60                  


Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Phe Val 
65                  70                  75                  80  


Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu 
                85                  90                  95      


Thr Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile 
            100                 105                 110         


Arg Glu Lys Leu Ile Gln Arg Ile Tyr Arg Gly Ile Glu Pro Thr Leu 
        115                 120                 125             


Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly 
    130                 135                 140                 


Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys 
145                 150                 155                 160 


Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu 
                165                 170                 175     


Ser Ala Cys Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His 
            180                 185                 190         


Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn 
        195                 200                 205             


Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr 
    210                 215                 220                 


Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys 
225                 230                 235                 240 


Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala 
                245                 250                 255     


Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys 
            260                 265                 270         


Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln 
        275                 280                 285             


Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu 
    290                 295                 300                 


Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala 
305                 310                 315                 320 


Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala 
                325                 330                 335     


Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro 
            340                 345                 350         


Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp 
        355                 360                 365             


Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala 
    370                 375                 380                 


Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg 
385                 390                 395                 400 


Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val 
                405                 410                 415     


Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser 
            420                 425                 430         


Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe 
        435                 440                 445             


Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln 
    450                 455                 460                 


Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val 
465                 470                 475                 480 


Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys Lys Arg Pro Ala 
                485                 490                 495     


Pro Ser Asp Ala Asp Ile Ser Glu Pro Lys Arg Val Arg Glu Ser Val 
            500                 505                 510         


Ala Gln Pro Ser Thr Ser Asp Ala Glu Ala Ser Ile Asn Tyr Ala Asp 
        515                 520                 525             


Arg Tyr Gln Asn Lys Cys Ser Arg His Val Gly Met Asn Leu Met Leu 
    530                 535                 540                 


Phe Pro Cys Arg Gln Cys Glu Arg Met Asn Gln Asn Ser Asn Ile Cys 
545                 550                 555                 560 


Phe Thr His Gly Gln Lys Asp Cys Leu Glu Cys Phe Pro Val Ser Glu 
                565                 570                 575     


Ser Gln Pro Val Ser Val Val Lys Lys Ala Tyr Gln Lys Leu Cys Tyr 
            580                 585                 590         


Ile His His Ile Met Gly Lys Val Pro Asp Ala Cys Thr Ala Cys Asp 
        595                 600                 605             


Leu Val Asn Val Asp Leu Asp Asp Cys Ile Phe Glu Gln 
    610                 615                 620     


<210>  5
<211>  1479
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pVD210 variant of Rep78 from AAV2


<220>
<221>  CDS
<222>  (1)..(1479)

<400>  5
acg gcg ggg ttc tac gag att gtg att aag gtc ccc agc gac ctt gac         48
Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp           
1               5                   10                  15                

gag cat ctg ccc ggc att tct gac agc ttt gtg aac tgg gtg gcc gag         96
Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu           
            20                  25                  30                    

aag gaa tgg gag ttg ccg cca gat tct gac atg gat ctg aat ctg att        144
Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile           
        35                  40                  45                        

gag cag gca ccc ctg acc gtg gcc gag aag ctg cag cgc gac ttt ctg        192
Glu Gln Ala Pro Leu Thr Val Ala Glu Lys Leu Gln Arg Asp Phe Leu           
    50                  55                  60                            

acg gaa tgg cgc cgt gtg agt aag gcc ccg gag gcc ctt ttc ttt gtg        240
Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Phe Val           
65                  70                  75                  80            

caa ttt gag aag gga gag agc tac ttc cac atg cac gtg ctc gtg gaa        288
Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu           
                85                  90                  95                

acc acc ggg gtg aaa tcc atg gtt ttg gga cgt ttc ctg agt cag att        336
Thr Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile           
            100                 105                 110                   

cgc gaa aaa ctg att cag aga att tac cgc ggg atc gag ccg act ttg        384
Arg Glu Lys Leu Ile Gln Arg Ile Tyr Arg Gly Ile Glu Pro Thr Leu           
        115                 120                 125                       

cca aac tgg ttc gcg gtc aca aag acc aga aat ggc gcc gga ggc ggg        432
Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly           
    130                 135                 140                           

aac aag gtg gtg gat gag tgc tac atc ccc aat tac ttg ctc ccc aaa        480
Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys           
145                 150                 155                 160           

acc cag cct gag ctc cag tgg gcg tgg act aat atg gaa cag tat tta        528
Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu           
                165                 170                 175               

agc gcc tgt ttg aat ctc acg gag cgt aaa cgg ttg gtg gcg cag cat        576
Ser Ala Cys Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His           
            180                 185                 190                   

ctg acg cac gtg tcg cag acg cag gag cag aac aaa gag aat cag aat        624
Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn           
        195                 200                 205                       

ccc aat tct gat gcg ccg gtg atc aga tca aaa act tca gcc agg tac        672
Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr           
    210                 215                 220                           

atg gag ctg gtc ggg tgg ctc gtg gac aag ggg att acc tcg gag aag        720
Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys           
225                 230                 235                 240           

cag tgg atc cag gag gac cag gcc tca tac atc tcc ttc aat gcg gcc        768
Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala           
                245                 250                 255               

tcc aac tcg cgg tcc caa atc aag gct gcc ttg gac aat gcg gga aag        816
Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys           
            260                 265                 270                   

att atg agc ctg act aaa acc gcc ccc gac tac ctg gtg ggc cag cag        864
Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln           
        275                 280                 285                       

ccc gtg gag gac att tcc agc aat cgg att tat aaa att ttg gaa cta        912
Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu           
    290                 295                 300                           

aac ggg tac gat ccc caa tat gcg gct tcc gtc ttt ctg gga tgg gcc        960
Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala           
305                 310                 315                 320           

acg aaa aag ttc ggc aag agg aac acc atc tgg ctg ttt ggg cct gca       1008
Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala           
                325                 330                 335               

act acc ggg aag acc aac atc gcg gag gcc ata gcc cac act gtg ccc       1056
Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro           
            340                 345                 350                   

ttc tac ggg tgc gta aac tgg acc aat gag aac ttt ccc ttc aac gac       1104
Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp           
        355                 360                 365                       

tgt gtc gac aag atg gtg atc tgg tgg gag gag ggg aag atg acc gcc       1152
Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala           
    370                 375                 380                           

aag gtc gtg gag tcg gcc aaa gcc att ctc gga gga agc aag gtg cgc       1200
Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg           
385                 390                 395                 400           

gtg gac cag aaa tgc aag tcc tcg gcc cag ata gac ccg act ccc gtg       1248
Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val           
                405                 410                 415               

atc gtc acc tcc aac acc aac atg tgc gcc gtg att gac ggg aac tca       1296
Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser           
            420                 425                 430                   

acg acc ttc gaa cac cag cag ccg ttg caa gac cgg atg ttc aaa ttt       1344
Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe           
        435                 440                 445                       

gaa ctc acc cgc cgt ctg gat cat gac ttt ggg aag gtc acc aag cag       1392
Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln           
    450                 455                 460                           

gaa gtc aaa gac ttt ttc cgg tgg gca aag gat cac gtg gtt gag gtg       1440
Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val           
465                 470                 475                 480           

gag cat gaa ttc tac gtc aaa aag ggt gga gcc aag taa                   1479
Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys                           
                485                 490                                   


<210>  6
<211>  492
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  6

Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp 
1               5                   10                  15      


Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu 
            20                  25                  30          


Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile 
        35                  40                  45              


Glu Gln Ala Pro Leu Thr Val Ala Glu Lys Leu Gln Arg Asp Phe Leu 
    50                  55                  60                  


Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Phe Val 
65                  70                  75                  80  


Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu 
                85                  90                  95      


Thr Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile 
            100                 105                 110         


Arg Glu Lys Leu Ile Gln Arg Ile Tyr Arg Gly Ile Glu Pro Thr Leu 
        115                 120                 125             


Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly 
    130                 135                 140                 


Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys 
145                 150                 155                 160 


Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu 
                165                 170                 175     


Ser Ala Cys Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His 
            180                 185                 190         


Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn 
        195                 200                 205             


Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr 
    210                 215                 220                 


Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys 
225                 230                 235                 240 


Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala 
                245                 250                 255     


Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys 
            260                 265                 270         


Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln 
        275                 280                 285             


Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu 
    290                 295                 300                 


Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala 
305                 310                 315                 320 


Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala 
                325                 330                 335     


Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro 
            340                 345                 350         


Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp 
        355                 360                 365             


Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala 
    370                 375                 380                 


Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg 
385                 390                 395                 400 


Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val 
                405                 410                 415     


Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser 
            420                 425                 430         


Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe 
        435                 440                 445             


Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln 
    450                 455                 460                 


Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val 
465                 470                 475                 480 


Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys 
                485                 490         


<210>  7
<211>  1866
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pVD211 variant of Rep78 from AAV2


<220>
<221>  CDS
<222>  (1)..(1866)

<400>  7
acg gcg ggg ttc tac gag att gtg att aag gtc ccc agc gac ctt gac         48
Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp           
1               5                   10                  15                

gag cat ctg ccc ggc att tct gac agc ttt gtg aac tgg gtg gcc gag         96
Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu           
            20                  25                  30                    

aag gaa tgg gag ttg ccg cca gat tct gac atg gat ctg aat ctg att        144
Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile           
        35                  40                  45                        

gag cag gca ccc ctg acc gtg gcc gag aag ctg cag cgc gac ttt ctg        192
Glu Gln Ala Pro Leu Thr Val Ala Glu Lys Leu Gln Arg Asp Phe Leu           
    50                  55                  60                            

acg gaa tgg cgc cgt gtg agt aag gcc ccg gag gcc ctt ttc tat gtg        240
Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Tyr Val           
65                  70                  75                  80            

caa ttt gag aag gga gag agc tac ttc cac atg cac gtg ctc gtg gaa        288
Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu           
                85                  90                  95                

acc acc ggg gtg aaa tcc atg gtt ttg gga cgt ttc ctg agt cag att        336
Thr Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile           
            100                 105                 110                   

cgc gaa aaa ctg att cag aga ttt tac cgc ggg atc gag ccg act ttg        384
Arg Glu Lys Leu Ile Gln Arg Phe Tyr Arg Gly Ile Glu Pro Thr Leu           
        115                 120                 125                       

cca aac tgg ttc gcg gtc aca aag acc aga aat ggc gcc gga ggc ggg        432
Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly           
    130                 135                 140                           

aac aag gtg gtg gat gag tgc tac atc ccc aat tac ttg ctc ccc aaa        480
Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys           
145                 150                 155                 160           

acc cag cct gag ctc cag tgg gcg tgg act aat atg gaa cag tat tta        528
Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu           
                165                 170                 175               

agc gcc tgt ttg aat ctc acg gag cgt aaa cgg ttg gtg gcg cag cat        576
Ser Ala Cys Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His           
            180                 185                 190                   

ctg acg cac gtg tcg cag acg cag gag cag aac aaa gag aat cag aat        624
Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn           
        195                 200                 205                       

ccc aat tct gat gcg ccg gtg atc aga tca aaa act tca gcc agg tac        672
Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr           
    210                 215                 220                           

atg gag ctg gtc ggg tgg ctc gtg gac aag ggg att acc tcg gag aag        720
Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys           
225                 230                 235                 240           

cag tgg atc cag gag gac cag gcc tca tac atc tcc ttc aat gcg gcc        768
Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala           
                245                 250                 255               

tcc aac tcg cgg tcc caa atc aag gct gcc ttg gac aat gcg gga aag        816
Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys           
            260                 265                 270                   

att atg agc ctg act aaa acc gcc ccc gac tac ctg gtg ggc cag cag        864
Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln           
        275                 280                 285                       

ccc gtg gag gac att tcc agc aat cgg att tat aaa att ttg gaa cta        912
Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu           
    290                 295                 300                           

aac ggg tac gat ccc caa tat gcg gct tcc gtc ttt ctg gga tgg gcc        960
Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala           
305                 310                 315                 320           

acg aaa aag ttc ggc aag agg aac acc atc tgg ctg ttt ggg cct gca       1008
Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala           
                325                 330                 335               

act acc ggg aag acc aac atc gcg gag gcc ata gcc cac act gtg ccc       1056
Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro           
            340                 345                 350                   

ttc tac ggg tgc gta aac tgg acc aat gag aac ttt ccc ttc aac gac       1104
Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp           
        355                 360                 365                       

tgt gtc gac aag atg gtg atc tgg tgg gag gag ggg aag atg acc gcc       1152
Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala           
    370                 375                 380                           

aag gtc gtg gag tcg gcc aaa gcc att ctc gga gga agc aag gtg cgc       1200
Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg           
385                 390                 395                 400           

gtg gac cag aaa tgc aag tcc tcg gcc cag ata gac ccg act ccc gtg       1248
Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val           
                405                 410                 415               

atc gtc acc tcc aac acc aac atg tgc gcc gtg att gac ggg aac tca       1296
Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser           
            420                 425                 430                   

acg acc ttc gaa cac cag cag ccg ttg caa gac cgg atg ttc aaa ttt       1344
Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe           
        435                 440                 445                       

gaa ctc acc cgc cgt ctg gat cat gac ttt ggg aag gtc acc aag cag       1392
Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln           
    450                 455                 460                           

gaa gtc aaa gac ttt ttc cgg tgg gca aag gat cac gtg gtt gag gtg       1440
Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val           
465                 470                 475                 480           

gag cat gaa ttc tac gtc aaa aag ggt gga gcc aag aaa aga ccc gcc       1488
Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys Lys Arg Pro Ala           
                485                 490                 495               

ccc agt gac gca gat ata agt gag ccc aaa cgg gtg cgc gag tca gtt       1536
Pro Ser Asp Ala Asp Ile Ser Glu Pro Lys Arg Val Arg Glu Ser Val           
            500                 505                 510                   

gcg cag cca tcg acg tca gac gcg gaa gct tcg atc aac tac gca gac       1584
Ala Gln Pro Ser Thr Ser Asp Ala Glu Ala Ser Ile Asn Tyr Ala Asp           
        515                 520                 525                       

agg tac caa aac aaa tgt tct cgt cac gtg ggc atg aat ctg atg ctg       1632
Arg Tyr Gln Asn Lys Cys Ser Arg His Val Gly Met Asn Leu Met Leu           
    530                 535                 540                           

ttt ccc tgc aga caa tgc gag aga atg aat cag aat tca aat atc tgc       1680
Phe Pro Cys Arg Gln Cys Glu Arg Met Asn Gln Asn Ser Asn Ile Cys           
545                 550                 555                 560           

ttc act cac gga cag aaa gac tgt tta gag tgc ttt ccc gtg tca gaa       1728
Phe Thr His Gly Gln Lys Asp Cys Leu Glu Cys Phe Pro Val Ser Glu           
                565                 570                 575               

tct caa ccc gtt tct gtc gtc aaa aag gcg tat cag aaa ctg tgc tac       1776
Ser Gln Pro Val Ser Val Val Lys Lys Ala Tyr Gln Lys Leu Cys Tyr           
            580                 585                 590                   

att cat cat atc atg gga aag gtg cca gac gct tgc act gcc tgc gat       1824
Ile His His Ile Met Gly Lys Val Pro Asp Ala Cys Thr Ala Cys Asp           
        595                 600                 605                       

ctg gtc aat gtg gat ttg gat gac tgc atc ttt gaa caa taa               1866
Leu Val Asn Val Asp Leu Asp Asp Cys Ile Phe Glu Gln                       
    610                 615                 620                           


<210>  8
<211>  621
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8

Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp 
1               5                   10                  15      


Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu 
            20                  25                  30          


Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile 
        35                  40                  45              


Glu Gln Ala Pro Leu Thr Val Ala Glu Lys Leu Gln Arg Asp Phe Leu 
    50                  55                  60                  


Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Tyr Val 
65                  70                  75                  80  


Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu 
                85                  90                  95      


Thr Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile 
            100                 105                 110         


Arg Glu Lys Leu Ile Gln Arg Phe Tyr Arg Gly Ile Glu Pro Thr Leu 
        115                 120                 125             


Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly 
    130                 135                 140                 


Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys 
145                 150                 155                 160 


Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu 
                165                 170                 175     


Ser Ala Cys Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His 
            180                 185                 190         


Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn 
        195                 200                 205             


Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr 
    210                 215                 220                 


Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys 
225                 230                 235                 240 


Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala 
                245                 250                 255     


Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys 
            260                 265                 270         


Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln 
        275                 280                 285             


Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu 
    290                 295                 300                 


Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala 
305                 310                 315                 320 


Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala 
                325                 330                 335     


Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro 
            340                 345                 350         


Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp 
        355                 360                 365             


Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala 
    370                 375                 380                 


Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg 
385                 390                 395                 400 


Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val 
                405                 410                 415     


Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser 
            420                 425                 430         


Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe 
        435                 440                 445             


Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln 
    450                 455                 460                 


Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val 
465                 470                 475                 480 


Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys Lys Arg Pro Ala 
                485                 490                 495     


Pro Ser Asp Ala Asp Ile Ser Glu Pro Lys Arg Val Arg Glu Ser Val 
            500                 505                 510         


Ala Gln Pro Ser Thr Ser Asp Ala Glu Ala Ser Ile Asn Tyr Ala Asp 
        515                 520                 525             


Arg Tyr Gln Asn Lys Cys Ser Arg His Val Gly Met Asn Leu Met Leu 
    530                 535                 540                 


Phe Pro Cys Arg Gln Cys Glu Arg Met Asn Gln Asn Ser Asn Ile Cys 
545                 550                 555                 560 


Phe Thr His Gly Gln Lys Asp Cys Leu Glu Cys Phe Pro Val Ser Glu 
                565                 570                 575     


Ser Gln Pro Val Ser Val Val Lys Lys Ala Tyr Gln Lys Leu Cys Tyr 
            580                 585                 590         


Ile His His Ile Met Gly Lys Val Pro Asp Ala Cys Thr Ala Cys Asp 
        595                 600                 605             


Leu Val Asn Val Asp Leu Asp Asp Cys Ile Phe Glu Gln 
    610                 615                 620     


<210>  9
<211>  1866
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pVD212 variant of Rep78 from AAV2


<220>
<221>  CDS
<222>  (1)..(1866)

<400>  9
acg gcg ggg ttc tac gag att gtg att aag gtc ccc agc gac ctt gac         48
Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp           
1               5                   10                  15                

gag cat ctg ccc ggc att tct gac agc ttt gtg aac tgg gtg gcc gag         96
Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu           
            20                  25                  30                    

aag gaa tgg gag ttg ccg cca gat tct gac atg gat ctg aat ctg att        144
Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile           
        35                  40                  45                        

gag cag gca ccc ctg acc gtg gcc ggg aag ctg cag cgc gac ttt ctg        192
Glu Gln Ala Pro Leu Thr Val Ala Gly Lys Leu Gln Arg Asp Phe Leu           
    50                  55                  60                            

acg gaa tgg cgc cgt gtg agt aag gcc ccg gag gcc ctt ttc ttt gtg        240
Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Phe Val           
65                  70                  75                  80            

caa ttt gag aag gga gag agc tac ttc cac atg cac gtg ctc gtg gaa        288
Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu           
                85                  90                  95                

ccc acc ggg gtg aaa tcc atg gtt ttg gga cgt ttc ctg agt cag att        336
Pro Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile           
            100                 105                 110                   

cgc gaa aaa ctg att cag aga att tac cgc ggg atc gag ccg act ttg        384
Arg Glu Lys Leu Ile Gln Arg Ile Tyr Arg Gly Ile Glu Pro Thr Leu           
        115                 120                 125                       

cca aac tgg ttc gcg gtc aca aag acc aga aat ggc gcc gga ggc ggg        432
Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly           
    130                 135                 140                           

aac aag gtg gtg gat gag tgc tac atc ccc aat tac ttg ctc ccc aaa        480
Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys           
145                 150                 155                 160           

acc cag cct gag ctc cag tgg gcg tgg act aat atg gaa cag tat tta        528
Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu           
                165                 170                 175               

agc gcc cgt ttg aat ctc acg gag cgt aaa cgg ttg gtg gcg cag cat        576
Ser Ala Arg Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His           
            180                 185                 190                   

ctg acg cac gtg tcg cag acg cag gag cag aac aaa gag aat cag aat        624
Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn           
        195                 200                 205                       

ccc aat tct gat gcg cct gtg atc aga tca aaa act tca gcc agg tac        672
Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr           
    210                 215                 220                           

atg gag ctg gtc ggg tgg ctc gtg gac aag ggg att acc tcg gag aag        720
Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys           
225                 230                 235                 240           

cag tgg atc cag gag gac cag gcc tca tac atc tcc ttc aat gcg gcc        768
Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala           
                245                 250                 255               

tcc aac tcg cgg tcc caa atc aag gct gcc ttg gac aat gcg gga aag        816
Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys           
            260                 265                 270                   

att atg agc ctg act aaa acc gcc ccc gac tac ctg gtg ggc cag cag        864
Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln           
        275                 280                 285                       

ccc gtg gag gac att tcc agc aat cgg att tat aaa att ttg gaa cta        912
Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu           
    290                 295                 300                           

aac ggg tac gat ccc caa tat gcg gct tcc gtc ttt ctg gga tgg gcc        960
Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala           
305                 310                 315                 320           

acg aaa aag ttc ggc aag agg aac acc atc tgg ctg ttt ggg cct gca       1008
Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala           
                325                 330                 335               

act acc ggg aag acc aac atc gcg gag gcc ata gcc cac act gtg ccc       1056
Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro           
            340                 345                 350                   

ttc tac ggg tgc gta aac tgg acc aat gag aac ttt ccc ttc aac gac       1104
Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp           
        355                 360                 365                       

tgt gtc gac aag atg gtg atc tgg tgg gag gag ggg aag atg acc gcc       1152
Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala           
    370                 375                 380                           

aag gtc gtg gag tcg gcc aaa gcc att ctc gga gga agc aag gtg cgc       1200
Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg           
385                 390                 395                 400           

gtg gac cag aaa tgc aag tcc tcg gcc cag ata gac ccg act ccc gtg       1248
Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val           
                405                 410                 415               

atc gtc acc tcc aac acc aac atg tgc gcc gtg att gac ggg aac tca       1296
Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser           
            420                 425                 430                   

acg acc ttc gaa cac cag cag ccg ttg caa gac cgg atg ttc aaa ttt       1344
Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe           
        435                 440                 445                       

gaa ctc acc cgc cgt ctg gat cat gac ttt ggg aag gtc acc aag cag       1392
Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln           
    450                 455                 460                           

gaa gtc aaa gac ttt ttc cgg tgg gca aag gat cac gtg gtt gag gtg       1440
Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val           
465                 470                 475                 480           

gag cat gaa ttc tac gtc aaa aag ggt gga gcc aag aaa aga ccc gcc       1488
Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys Lys Arg Pro Ala           
                485                 490                 495               

ccc agt gac gca gat ata agt gag ccc aaa cgg gtg cgc gag tca gtt       1536
Pro Ser Asp Ala Asp Ile Ser Glu Pro Lys Arg Val Arg Glu Ser Val           
            500                 505                 510                   

gcg cag cca tcg acg tca gac gcg gaa gct tcg atc aac tac gca gac       1584
Ala Gln Pro Ser Thr Ser Asp Ala Glu Ala Ser Ile Asn Tyr Ala Asp           
        515                 520                 525                       

agg tac caa aac aaa tgt tct cgt cac gtg ggc atg aat ctg atg ctg       1632
Arg Tyr Gln Asn Lys Cys Ser Arg His Val Gly Met Asn Leu Met Leu           
    530                 535                 540                           

ttt ccc tgc aga caa tgc gag aga atg aat cag aat tca aat atc tgc       1680
Phe Pro Cys Arg Gln Cys Glu Arg Met Asn Gln Asn Ser Asn Ile Cys           
545                 550                 555                 560           

ttc act cac gga cag aaa gac tgt tta gag tgc ttt ccc gtg tca gaa       1728
Phe Thr His Gly Gln Lys Asp Cys Leu Glu Cys Phe Pro Val Ser Glu           
                565                 570                 575               

tct caa ccc gtt tct gtc gtc aaa aag gcg tat cag aaa ctg tgc tac       1776
Ser Gln Pro Val Ser Val Val Lys Lys Ala Tyr Gln Lys Leu Cys Tyr           
            580                 585                 590                   

att cat cat atc atg gga aag gtg cca gac gct tgc act gcc tgc gat       1824
Ile His His Ile Met Gly Lys Val Pro Asp Ala Cys Thr Ala Cys Asp           
        595                 600                 605                       

ctg gtc aat gtg gat ttg gat gac tgc atc ttt gaa caa taa               1866
Leu Val Asn Val Asp Leu Asp Asp Cys Ile Phe Glu Gln                       
    610                 615                 620                           


<210>  10
<211>  621
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  10

Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp 
1               5                   10                  15      


Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu 
            20                  25                  30          


Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile 
        35                  40                  45              


Glu Gln Ala Pro Leu Thr Val Ala Gly Lys Leu Gln Arg Asp Phe Leu 
    50                  55                  60                  


Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Phe Val 
65                  70                  75                  80  


Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu 
                85                  90                  95      


Pro Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile 
            100                 105                 110         


Arg Glu Lys Leu Ile Gln Arg Ile Tyr Arg Gly Ile Glu Pro Thr Leu 
        115                 120                 125             


Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly 
    130                 135                 140                 


Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys 
145                 150                 155                 160 


Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu 
                165                 170                 175     


Ser Ala Arg Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His 
            180                 185                 190         


Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn 
        195                 200                 205             


Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr 
    210                 215                 220                 


Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys 
225                 230                 235                 240 


Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala 
                245                 250                 255     


Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys 
            260                 265                 270         


Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln 
        275                 280                 285             


Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu 
    290                 295                 300                 


Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala 
305                 310                 315                 320 


Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala 
                325                 330                 335     


Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro 
            340                 345                 350         


Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp 
        355                 360                 365             


Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala 
    370                 375                 380                 


Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg 
385                 390                 395                 400 


Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val 
                405                 410                 415     


Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser 
            420                 425                 430         


Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe 
        435                 440                 445             


Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln 
    450                 455                 460                 


Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val 
465                 470                 475                 480 


Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys Lys Arg Pro Ala 
                485                 490                 495     


Pro Ser Asp Ala Asp Ile Ser Glu Pro Lys Arg Val Arg Glu Ser Val 
            500                 505                 510         


Ala Gln Pro Ser Thr Ser Asp Ala Glu Ala Ser Ile Asn Tyr Ala Asp 
        515                 520                 525             


Arg Tyr Gln Asn Lys Cys Ser Arg His Val Gly Met Asn Leu Met Leu 
    530                 535                 540                 


Phe Pro Cys Arg Gln Cys Glu Arg Met Asn Gln Asn Ser Asn Ile Cys 
545                 550                 555                 560 


Phe Thr His Gly Gln Lys Asp Cys Leu Glu Cys Phe Pro Val Ser Glu 
                565                 570                 575     


Ser Gln Pro Val Ser Val Val Lys Lys Ala Tyr Gln Lys Leu Cys Tyr 
            580                 585                 590         


Ile His His Ile Met Gly Lys Val Pro Asp Ala Cys Thr Ala Cys Asp 
        595                 600                 605             


Leu Val Asn Val Asp Leu Asp Asp Cys Ile Phe Glu Gln 
    610                 615                 620     


<210>  11
<211>  1713
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pVD214 variant of Rep78 from AAV2


<220>
<221>  CDS
<222>  (1)..(1713)

<400>  11
acg gcg ggg ttc tac gag att gtg att aag gtc ccc agc gac ctt gac         48
Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp           
1               5                   10                  15                

gag cat ctg ccc ggc att tct gac agc ttt gtg aac tgg gtg gcc gag         96
Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu           
            20                  25                  30                    

aag gaa tgg gag ttg ccg cca gat tct gac atg gat ctg aat ctg att        144
Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile           
        35                  40                  45                        

gag cag gca ccc ctg acc gtg gcc ggg aag ctg cag cgc gac ttt ctg        192
Glu Gln Ala Pro Leu Thr Val Ala Gly Lys Leu Gln Arg Asp Phe Leu           
    50                  55                  60                            

acg gaa tgg cgc cgt gtg agt aag gcc ccg gag gcc ctt ttc ttt gtg        240
Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Phe Val           
65                  70                  75                  80            

caa ttt gag aag gga gag agc tac ttc cac atg cac gtg ctc gtg gaa        288
Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu           
                85                  90                  95                

ccc acc ggg gtg aaa tcc atg gtt ttg gga cgt ttc ctg agt cag att        336
Pro Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile           
            100                 105                 110                   

cgc gaa aaa ctg att cag aga att tac cgc ggg atc gag ccg act ttg        384
Arg Glu Lys Leu Ile Gln Arg Ile Tyr Arg Gly Ile Glu Pro Thr Leu           
        115                 120                 125                       

cca aac tgg ttc gcg gtc aca aag acc aga aat ggc gcc gga ggc ggg        432
Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly           
    130                 135                 140                           

aac aag gtg gtg gat gag tgc tac atc ccc aat tac ttg ctc ccc aaa        480
Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys           
145                 150                 155                 160           

acc cag cct gag ctc cag tgg gcg tgg act aat atg gaa cag tat tta        528
Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu           
                165                 170                 175               

agc gcc cgt ttg aat ctc acg gag cgt aaa cgg ttg gtg gcg cag cat        576
Ser Ala Arg Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His           
            180                 185                 190                   

ctg acg cac gtg tcg cag acg cag gag cag aac aaa gag aat cag aat        624
Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn           
        195                 200                 205                       

ccc aat tct gat gcg cct gtg atc aga tca aaa act tca gcc agg tac        672
Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr           
    210                 215                 220                           

atg gag ctg gtc ggg tgg ctc gtg gac aag ggg att acc tcg gag aag        720
Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys           
225                 230                 235                 240           

cag tgg atc cag gag gac cag gcc tca tac atc tcc ttc aat gcg gcc        768
Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala           
                245                 250                 255               

tcc aac tcg cgg tcc caa atc aag gct gcc ttg gac aat gcg gga aag        816
Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys           
            260                 265                 270                   

att atg agc ctg act aaa acc gcc ccc gac tac ctg gtg ggc cag cag        864
Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln           
        275                 280                 285                       

ccc gtg gag gac att tcc agc aat cgg ata tat aaa att ttg gaa cta        912
Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu           
    290                 295                 300                           

aac ggg tac gat ccc caa tat gcg gct tcc gtc ttt ctg gga tgg gcc        960
Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala           
305                 310                 315                 320           

acg aaa aag ttc ggc aag agg aac acc atc tgg ctg ttt ggg cct gca       1008
Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala           
                325                 330                 335               

act acc ggg aag acc aac atc gcg gag gcc ata gcc cac act gtg ccc       1056
Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro           
            340                 345                 350                   

ttc tac ggg tgc gta aac tgg acc aat gag aac ttt ccc ttc aac gac       1104
Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp           
        355                 360                 365                       

tgt gtc gac aag atg gtg atc tgg tgg gag gag ggg aag atg acc gcc       1152
Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala           
    370                 375                 380                           

aag gtc gtg gag tcg gcc aaa gcc att ctc gga gga agc aag gtg cgc       1200
Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg           
385                 390                 395                 400           

gtg gac cag aaa tgc aag tcc tcg gcc cag ata gac ccg act ccc gtg       1248
Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val           
                405                 410                 415               

atc gtc acc tcc aac acc aac atg tgc gcc gtg att gac ggg aac tca       1296
Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser           
            420                 425                 430                   

acg acc ttc gaa cac cag cag ccg ttg caa gac cgg atg ttc aaa ttt       1344
Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe           
        435                 440                 445                       

gaa ctc acc cgc cgt ctg gat cat gac ttt ggg aag gtc acc aag cag       1392
Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln           
    450                 455                 460                           

gaa gtc aaa gac ttt ttc cgg tgg gca aag gat cac gtg gtt gag gtg       1440
Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val           
465                 470                 475                 480           

gag cat gaa ctc tac gtc aaa aag ggt gga gcc aag aaa aga ccc gcc       1488
Glu His Glu Leu Tyr Val Lys Lys Gly Gly Ala Lys Lys Arg Pro Ala           
                485                 490                 495               

ccc agt gac gca gat ata agt gag ccc aaa cgg gtg cgc gag tca gtt       1536
Pro Ser Asp Ala Asp Ile Ser Glu Pro Lys Arg Val Arg Glu Ser Val           
            500                 505                 510                   

gcg cag cca tcg acg tca gac gcg gaa gct tcg atc aac tac gca gac       1584
Ala Gln Pro Ser Thr Ser Asp Ala Glu Ala Ser Ile Asn Tyr Ala Asp           
        515                 520                 525                       

agg tac caa aac aaa tgt tct cgt cac gtg ggc atg aat ctg atg ctg       1632
Arg Tyr Gln Asn Lys Cys Ser Arg His Val Gly Met Asn Leu Met Leu           
    530                 535                 540                           

ttt ccc tgc aga caa tgc gag aga atg aat cag aat tca aat atc tgc       1680
Phe Pro Cys Arg Gln Cys Glu Arg Met Asn Gln Asn Ser Asn Ile Cys           
545                 550                 555                 560           

ttc act cac gga cag aaa gac tgt tta gag tga                           1713
Phe Thr His Gly Gln Lys Asp Cys Leu Glu                                   
                565                 570                                   


<210>  12
<211>  570
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  12

Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp 
1               5                   10                  15      


Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu 
            20                  25                  30          


Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile 
        35                  40                  45              


Glu Gln Ala Pro Leu Thr Val Ala Gly Lys Leu Gln Arg Asp Phe Leu 
    50                  55                  60                  


Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Phe Val 
65                  70                  75                  80  


Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu 
                85                  90                  95      


Pro Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile 
            100                 105                 110         


Arg Glu Lys Leu Ile Gln Arg Ile Tyr Arg Gly Ile Glu Pro Thr Leu 
        115                 120                 125             


Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly 
    130                 135                 140                 


Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys 
145                 150                 155                 160 


Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu 
                165                 170                 175     


Ser Ala Arg Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His 
            180                 185                 190         


Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn 
        195                 200                 205             


Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr 
    210                 215                 220                 


Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys 
225                 230                 235                 240 


Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala 
                245                 250                 255     


Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys 
            260                 265                 270         


Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln 
        275                 280                 285             


Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu 
    290                 295                 300                 


Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala 
305                 310                 315                 320 


Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala 
                325                 330                 335     


Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro 
            340                 345                 350         


Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp 
        355                 360                 365             


Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala 
    370                 375                 380                 


Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg 
385                 390                 395                 400 


Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val 
                405                 410                 415     


Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser 
            420                 425                 430         


Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe 
        435                 440                 445             


Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln 
    450                 455                 460                 


Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val 
465                 470                 475                 480 


Glu His Glu Leu Tyr Val Lys Lys Gly Gly Ala Lys Lys Arg Pro Ala 
                485                 490                 495     


Pro Ser Asp Ala Asp Ile Ser Glu Pro Lys Arg Val Arg Glu Ser Val 
            500                 505                 510         


Ala Gln Pro Ser Thr Ser Asp Ala Glu Ala Ser Ile Asn Tyr Ala Asp 
        515                 520                 525             


Arg Tyr Gln Asn Lys Cys Ser Arg His Val Gly Met Asn Leu Met Leu 
    530                 535                 540                 


Phe Pro Cys Arg Gln Cys Glu Arg Met Asn Gln Asn Ser Asn Ile Cys 
545                 550                 555                 560 


Phe Thr His Gly Gln Lys Asp Cys Leu Glu 
                565                 570 


<210>  13
<211>  1479
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pVD215 variant of Rep78 from AAV2


<220>
<221>  CDS
<222>  (1)..(1479)

<400>  13
acg gcg ggg ttc tac gag att gtg att aag gtc ccc agc gac ctt gac         48
Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp           
1               5                   10                  15                

gag cat ctg ccc ggc att tct gac agc ttt gtg aac tgg gtg gcc gag         96
Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu           
            20                  25                  30                    

aag gaa tgg gag ttg ccg cca gat tct gac gtg gat ctg aat ctg att        144
Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Val Asp Leu Asn Leu Ile           
        35                  40                  45                        

gag cag gca ccc ctg acc gtg gcc gag aag ctg cag cgc gac ttt ctg        192
Glu Gln Ala Pro Leu Thr Val Ala Glu Lys Leu Gln Arg Asp Phe Leu           
    50                  55                  60                            

acg gaa tgg cgc cgt gtg agt aag gcc ccg gag gcc ctt ttc tat gtg        240
Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Tyr Val           
65                  70                  75                  80            

caa ttt gag aag gga gag agc tac ttc cac atg cac gtg ctc gtg gaa        288
Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu           
                85                  90                  95                

acc acc ggg gtg aaa tcc atg gtt ttg gga cgt ttc ctg agt cag att        336
Thr Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile           
            100                 105                 110                   

cgc gaa aaa ctg att cag aga ttt tac cgc ggg atc gag ccg act ttg        384
Arg Glu Lys Leu Ile Gln Arg Phe Tyr Arg Gly Ile Glu Pro Thr Leu           
        115                 120                 125                       

cca aac tgg ttc gcg gtc aca aag acc aga aat ggc gcc gga ggc ggg        432
Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly           
    130                 135                 140                           

aac aag gtg gtg gat gag tgc tac atc ccc aat tac ttg ctc ccc aaa        480
Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys           
145                 150                 155                 160           

acc cag cct gag ctc cag tgg gcg tgg act aat atg gaa cag tat tta        528
Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu           
                165                 170                 175               

agc gcc tgt ttg aat ctc acg gag cgt aaa cgg ttg gtg gcg cag cat        576
Ser Ala Cys Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His           
            180                 185                 190                   

ctg acg cac gtg tcg cag acg cag gag cag aac aaa gag aat cag aat        624
Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn           
        195                 200                 205                       

ccc aat tcc gat gcg ccg gtg atc aga tca aaa act tca gcc agg tac        672
Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr           
    210                 215                 220                           

atg gag ctg gtc ggg tgg ctc gtg gac aag ggg att acc tcg gag aag        720
Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys           
225                 230                 235                 240           

cag tgg atc cag gag gac cag gcc tca tac atc tcc ttc aat gcg gcc        768
Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala           
                245                 250                 255               

tcc aac tcg cgg tcc caa atc aag gct gcc ttg gac aat gcg gga aag        816
Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys           
            260                 265                 270                   

att atg agc ctg act aaa acc gcc ccc gac tac ctg gtg ggc cag cag        864
Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln           
        275                 280                 285                       

ccc gtg gag gac att tcc agc aat cgg att tat aaa att ttg gaa cta        912
Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu           
    290                 295                 300                           

aac ggg tac gat ccc caa tat gcg gct tcc gtc ttt ctg gga tgg gcc        960
Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala           
305                 310                 315                 320           

acg aaa aag ttc ggc aag agg aac acc atc tgg ctg ttt ggg cct gca       1008
Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala           
                325                 330                 335               

act acc ggg aag acc aac atc gcg gag gcc ata gcc cac act gtg ccc       1056
Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro           
            340                 345                 350                   

ttc tac ggg tgc gta aac tgg acc aat gag aac ttt ccc ttc aac gac       1104
Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp           
        355                 360                 365                       

tgt gtc gac aag atg gtg atc tgg tgg gag gag ggg aag atg acc gcc       1152
Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala           
    370                 375                 380                           

aag gtc gtg gag tcg gcc aaa gcc att ctc gga gga agc aag gtg cgc       1200
Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg           
385                 390                 395                 400           

gtg gac cag aaa tgc aag tcc tcg gcc cag ata gac ccg act ccc gtg       1248
Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val           
                405                 410                 415               

atc gtc acc tcc aac acc aac atg tgc gcc gtg att gac ggg aac tca       1296
Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser           
            420                 425                 430                   

acg acc ttc gaa cac cag cag ccg ttg caa gac cgg atg ttc aaa ttt       1344
Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe           
        435                 440                 445                       

gaa ctc acc cgc cgt ctg gat cat gac ttt ggg aag gtc acc aag cag       1392
Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln           
    450                 455                 460                           

gaa gtc aaa gac ttt ttc cgg tgg gca aag gat cac gtg gtt gag gtg       1440
Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val           
465                 470                 475                 480           

gag cat gaa ttc tac gtc aaa aag ggt gga gcc aag taa                   1479
Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys                           
                485                 490                                   


<210>  14
<211>  492
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  14

Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp 
1               5                   10                  15      


Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu 
            20                  25                  30          


Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Val Asp Leu Asn Leu Ile 
        35                  40                  45              


Glu Gln Ala Pro Leu Thr Val Ala Glu Lys Leu Gln Arg Asp Phe Leu 
    50                  55                  60                  


Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Tyr Val 
65                  70                  75                  80  


Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu 
                85                  90                  95      


Thr Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile 
            100                 105                 110         


Arg Glu Lys Leu Ile Gln Arg Phe Tyr Arg Gly Ile Glu Pro Thr Leu 
        115                 120                 125             


Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly 
    130                 135                 140                 


Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys 
145                 150                 155                 160 


Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu 
                165                 170                 175     


Ser Ala Cys Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His 
            180                 185                 190         


Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn 
        195                 200                 205             


Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr 
    210                 215                 220                 


Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys 
225                 230                 235                 240 


Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala 
                245                 250                 255     


Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys 
            260                 265                 270         


Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln 
        275                 280                 285             


Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu 
    290                 295                 300                 


Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala 
305                 310                 315                 320 


Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala 
                325                 330                 335     


Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro 
            340                 345                 350         


Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp 
        355                 360                 365             


Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala 
    370                 375                 380                 


Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg 
385                 390                 395                 400 


Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val 
                405                 410                 415     


Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser 
            420                 425                 430         


Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe 
        435                 440                 445             


Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln 
    450                 455                 460                 


Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val 
465                 470                 475                 480 


Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys 
                485                 490         


<210>  15
<211>  1479
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pVD216 variant of Rep78 from AAV2


<220>
<221>  CDS
<222>  (1)..(1479)

<400>  15
acg gcg ggg ttc tac gag att gtg att aag gtc ccc agc gac ctt gac         48
Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp           
1               5                   10                  15                

gag cat ctg ccc ggc att tct gac agc ttt gtg aac tgg gtg gcc gag         96
Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu           
            20                  25                  30                    

aag gaa tgg gag ttg ccg cca gat tct gac gtg gat ctg aat ctg att        144
Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Val Asp Leu Asn Leu Ile           
        35                  40                  45                        

gag cag gca ccc ctg acc gtg gcc gag aag ctg cag cgc gac ttt ctg        192
Glu Gln Ala Pro Leu Thr Val Ala Glu Lys Leu Gln Arg Asp Phe Leu           
    50                  55                  60                            

acg gaa tgg cgc cgt gtg agt aag gcc ccg gag gcc ctt ttc tat gtg        240
Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Tyr Val           
65                  70                  75                  80            

caa ttt gag aag gga gag agc tac ttc cac atg cac gtg ctc gtg gaa        288
Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu           
                85                  90                  95                

acc acc ggg gtg aaa tcc atg gtt ttg gga cgt ttc ctg agt cag att        336
Thr Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile           
            100                 105                 110                   

cgc gaa aaa ctg att cag aga ttt tac cgc ggg atc gag ccg act ttg        384
Arg Glu Lys Leu Ile Gln Arg Phe Tyr Arg Gly Ile Glu Pro Thr Leu           
        115                 120                 125                       

cca aac tgg ttc gcg gtc aca aag acc aga aat ggc gcc gga ggc ggg        432
Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly           
    130                 135                 140                           

aac aag gtg gtg gat gag tgc tac atc ccc aat tac ttg ctc ccc aaa        480
Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys           
145                 150                 155                 160           

acc cag cct gag ctc cag tgg gcg tgg act aat atg gaa cag tat tta        528
Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu           
                165                 170                 175               

agc gcc tgt ttg aat ctc acg gag cgt aaa cgg ttg gtg gcg cag cat        576
Ser Ala Cys Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His           
            180                 185                 190                   

ctg acg cac gtg tcg cag acg cag gag cag aac aaa gag aat cag aat        624
Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn           
        195                 200                 205                       

ccc aat tct gat gcg ccg gtg atc aga tca aaa act tca gcc agg tac        672
Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr           
    210                 215                 220                           

atg gag ctg gtc ggg tgg ctc gtg gac aag ggg att acc tcg gag aag        720
Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys           
225                 230                 235                 240           

cag tgg atc cag gag gac cag gcc tca tac atc tcc ttc aat gcg gcc        768
Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala           
                245                 250                 255               

tcc aac tcg cgg tcc caa atc aag gct gcc ttg gac aat gcg gga aag        816
Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys           
            260                 265                 270                   

att atg agc ctg act aaa acc gcc ccc gac tac ctg gtg ggc cag cag        864
Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln           
        275                 280                 285                       

ccc gtg gag gac att tcc agc aat cgg att tat aaa att ttg gaa cta        912
Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu           
    290                 295                 300                           

aac ggg tac gat ccc caa tat gcg gct tcc gtc ttt ctg gga tgg gcc        960
Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala           
305                 310                 315                 320           

acg aaa aag ttc ggc aag agg aac acc atc tgg ctg ttt ggg cct gca       1008
Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala           
                325                 330                 335               

act acc ggg aag acc aac atc gcg gag gcc ata gcc cac act gtg ccc       1056
Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro           
            340                 345                 350                   

ttc tac ggg tgc gta aac tgg acc aat gag aac ttt ccc ttc aac gac       1104
Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp           
        355                 360                 365                       

tgt gtc gac aag atg gtg atc tgg tgg gag gag ggg aag atg acc gcc       1152
Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala           
    370                 375                 380                           

aag gtc gtg gag tcg gcc aaa gcc att ctc gga gga agc aag gtg cgc       1200
Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg           
385                 390                 395                 400           

gtg gac cag aaa tgc aag tcc tcg gcc cag ata gac ccg act ccc gtg       1248
Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val           
                405                 410                 415               

atc gtc acc tcc aac acc aac atg tgc gcc gtg att gac ggg aac tca       1296
Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser           
            420                 425                 430                   

acg acc ttc gaa cac cag cag ccg ttg caa gac cgg atg ttc aaa ttt       1344
Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe           
        435                 440                 445                       

gaa ctc acc cgc cgt ctg gat cat gac ttt ggg aag gtc acc aag cag       1392
Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln           
    450                 455                 460                           

gaa gtc aaa gac ttt ttc cgg tgg gca aag gat cac gtg gtt gag gtg       1440
Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val           
465                 470                 475                 480           

gag cat gaa ttc tac gtc aaa aag ggt gga gcc aag taa                   1479
Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys                           
                485                 490                                   


<210>  16
<211>  492
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  16

Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp 
1               5                   10                  15      


Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu 
            20                  25                  30          


Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Val Asp Leu Asn Leu Ile 
        35                  40                  45              


Glu Gln Ala Pro Leu Thr Val Ala Glu Lys Leu Gln Arg Asp Phe Leu 
    50                  55                  60                  


Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Tyr Val 
65                  70                  75                  80  


Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu 
                85                  90                  95      


Thr Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile 
            100                 105                 110         


Arg Glu Lys Leu Ile Gln Arg Phe Tyr Arg Gly Ile Glu Pro Thr Leu 
        115                 120                 125             


Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly 
    130                 135                 140                 


Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys 
145                 150                 155                 160 


Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu 
                165                 170                 175     


Ser Ala Cys Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His 
            180                 185                 190         


Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn 
        195                 200                 205             


Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr 
    210                 215                 220                 


Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys 
225                 230                 235                 240 


Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala 
                245                 250                 255     


Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys 
            260                 265                 270         


Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln 
        275                 280                 285             


Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu 
    290                 295                 300                 


Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala 
305                 310                 315                 320 


Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala 
                325                 330                 335     


Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro 
            340                 345                 350         


Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp 
        355                 360                 365             


Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala 
    370                 375                 380                 


Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg 
385                 390                 395                 400 


Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val 
                405                 410                 415     


Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser 
            420                 425                 430         


Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe 
        435                 440                 445             


Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln 
    450                 455                 460                 


Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val 
465                 470                 475                 480 


Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys 
                485                 490         


<210>  17
<211>  1479
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pVD217 variant of Rep78 from AAV2


<220>
<221>  CDS
<222>  (1)..(1479)

<400>  17
acg gcg ggg ttc tac gag att gtg att aag gtc ccc agc gac ctt gac         48
Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp           
1               5                   10                  15                

gag cat ctg ccc ggc att tct gac agc ttt gtg aac tgg gtg gcc gag         96
Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu           
            20                  25                  30                    

aag gaa tgg gag ttg ccg cca gat tct gac atg gat ctg aat ctg att        144
Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile           
        35                  40                  45                        

gag cag gca ccc ctg acc gtg gcc gag aag ctg cag cgc gac ttt ctg        192
Glu Gln Ala Pro Leu Thr Val Ala Glu Lys Leu Gln Arg Asp Phe Leu           
    50                  55                  60                            

acg gaa tgg cgc cgt gtg agt aag gcc ccg gag gcc ctt ttc tat gtg        240
Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Tyr Val           
65                  70                  75                  80            

caa ttt gag aag gga gag agc tac ttc cac atg cac gtg ctc gtg gaa        288
Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu           
                85                  90                  95                

acc acc ggg gtg aaa tcc atg gtt ttg gga cgt ttc ctg agt cag att        336
Thr Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile           
            100                 105                 110                   

cgc gaa aaa ctg att cag aga ttt tac cgc ggg atc gag ccg act ttg        384
Arg Glu Lys Leu Ile Gln Arg Phe Tyr Arg Gly Ile Glu Pro Thr Leu           
        115                 120                 125                       

cca aac tgg ttc gcg gtc aca aag acc aga aat ggc gcc gga ggc ggg        432
Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly           
    130                 135                 140                           

aac aag gtg gtg gat gag tgc tac atc ccc aat tac ttg ctc ccc aaa        480
Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys           
145                 150                 155                 160           

acc cag cct gag ctc cag tgg gcg tgg act aat atg gaa cag tat tta        528
Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu           
                165                 170                 175               

agc gcc tgt ttg aat ctc acg gag cgt aaa cgg ttg gtg gcg cag cat        576
Ser Ala Cys Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His           
            180                 185                 190                   

ctg acg cac gtg tcg cag acg cag gag cag aac aaa gag aat cag aat        624
Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn           
        195                 200                 205                       

ccc aat tct gat gcg ccg gtg atc aga tca aaa act tca gcc agg tac        672
Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr           
    210                 215                 220                           

atg gag ctg gtc ggg tgg ctc gtg gac aag ggg att acc tcg gag aag        720
Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys           
225                 230                 235                 240           

cag tgg atc cag gag gac cag gcc tca tac atc tcc ttc aat gcg gcc        768
Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala           
                245                 250                 255               

tcc aac tcg cgg tcc caa atc aag gct gcc ttg gac aat gcg gga aag        816
Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys           
            260                 265                 270                   

att atg agc ctg act aaa acc gcc ccc gac tac ctg gtg ggc cag cag        864
Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln           
        275                 280                 285                       

ccc gtg gag gac att tcc agc aat cgg att tat aaa att ttg gaa cta        912
Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu           
    290                 295                 300                           

aac ggg tac gat ccc caa tat gcg gct tcc gtc ttt ctg gga tgg gcc        960
Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala           
305                 310                 315                 320           

acg aaa aag ttc ggc aag agg aac acc atc tgg ctg ttt ggg cct gca       1008
Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala           
                325                 330                 335               

act acc ggg aag acc aac atc gcg gag gcc ata gcc cac act gtg ccc       1056
Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro           
            340                 345                 350                   

ttc tac ggg tgc gta aac tgg acc aat gag aac ttt ccc ttc aac gac       1104
Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp           
        355                 360                 365                       

tgt gtc gac aag atg gtg atc tgg tgg gag gag ggg aag atg acc gcc       1152
Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala           
    370                 375                 380                           

aag gtc gtg gag tcg gcc aaa gcc att ctc gga gga agc aag gtg cgc       1200
Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg           
385                 390                 395                 400           

gtg gac cag aaa tgc aag tcc tcg gcc cag ata gac ccg act ccc gtg       1248
Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val           
                405                 410                 415               

atc gtc acc tcc aac acc aac atg tgc gcc gtg att gac ggg aac tca       1296
Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser           
            420                 425                 430                   

acg acc ttc gaa cac cag cag ccg ttg caa gac cgg atg ttc aaa ttt       1344
Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe           
        435                 440                 445                       

gaa ctc acc cgc cgt ctg gat cat gac ttt ggg aag gtc acc aag cag       1392
Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln           
    450                 455                 460                           

gaa gtc aaa gac ttt ttc cgg tgg gca aag gat cac gtg gtt gag gtg       1440
Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val           
465                 470                 475                 480           

gag cat gaa ttc tac gtc aaa aag ggt gga gcc aag taa                   1479
Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys                           
                485                 490                                   


<210>  18
<211>  492
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  18

Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp 
1               5                   10                  15      


Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu 
            20                  25                  30          


Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile 
        35                  40                  45              


Glu Gln Ala Pro Leu Thr Val Ala Glu Lys Leu Gln Arg Asp Phe Leu 
    50                  55                  60                  


Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Tyr Val 
65                  70                  75                  80  


Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu 
                85                  90                  95      


Thr Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile 
            100                 105                 110         


Arg Glu Lys Leu Ile Gln Arg Phe Tyr Arg Gly Ile Glu Pro Thr Leu 
        115                 120                 125             


Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly 
    130                 135                 140                 


Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys 
145                 150                 155                 160 


Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu 
                165                 170                 175     


Ser Ala Cys Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His 
            180                 185                 190         


Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn 
        195                 200                 205             


Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr 
    210                 215                 220                 


Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys 
225                 230                 235                 240 


Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala 
                245                 250                 255     


Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys 
            260                 265                 270         


Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln 
        275                 280                 285             


Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu 
    290                 295                 300                 


Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala 
305                 310                 315                 320 


Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala 
                325                 330                 335     


Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro 
            340                 345                 350         


Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp 
        355                 360                 365             


Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala 
    370                 375                 380                 


Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg 
385                 390                 395                 400 


Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val 
                405                 410                 415     


Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser 
            420                 425                 430         


Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe 
        435                 440                 445             


Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln 
    450                 455                 460                 


Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val 
465                 470                 475                 480 


Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys 
                485                 490         


<210>  19
<211>  1479
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pVD218 variant of Rep78 from AAV2


<220>
<221>  CDS
<222>  (1)..(1479)

<400>  19
acg gcg ggg ttc tac gag att gtg att aag gtc ccc agc gac ctt gac         48
Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp           
1               5                   10                  15                

gag cat ctg ccc ggc att tct gac agc ttt gtg aac tgg gtg gcc gag         96
Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu           
            20                  25                  30                    

aag gaa tgg gag ttg ccg cca gat tct gac atg gat ctg aat ctg att        144
Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile           
        35                  40                  45                        

gag cag gca ccc ctg acc gtg gcc gag aag ctg cag cgc gac ttt ctg        192
Glu Gln Ala Pro Leu Thr Val Ala Glu Lys Leu Gln Arg Asp Phe Leu           
    50                  55                  60                            

acg gaa tgg cgc cgt gtg agt aag gcc ccg gag gcc ctt ttc tat gtg        240
Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Tyr Val           
65                  70                  75                  80            

caa ttt gag aag gga gag agc tac ttc cac atg cac gtg ctc gtg gaa        288
Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu           
                85                  90                  95                

acc acc ggg gtg aaa tcc atg gtt ttg gga cgt ttc ctg agt cag att        336
Thr Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile           
            100                 105                 110                   

cgc gaa aaa ctg att cag aga ttt tac cgc ggg atc gag ccg act ttg        384
Arg Glu Lys Leu Ile Gln Arg Phe Tyr Arg Gly Ile Glu Pro Thr Leu           
        115                 120                 125                       

cca aac tgg ttc gcg gtc aca aag acc aga aat ggc gcc gga ggc ggg        432
Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly           
    130                 135                 140                           

aac aag gtg gtg gat gag tgc tac atc ccc aat tac ttg ctc ccc aaa        480
Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys           
145                 150                 155                 160           

acc cag cct gag ctc cag tgg gcg tgg act aat atg gaa cag tat tta        528
Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu           
                165                 170                 175               

agc gcc tgt ttg aat ctc acg gag cgt aaa cgg ttg gtg gcg cag cat        576
Ser Ala Cys Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His           
            180                 185                 190                   

ctg acg cac gtg tcg cag acg cag gag cag aac aaa gag aat cag aat        624
Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn           
        195                 200                 205                       

ccc aat tct gat gcg ccg gtg atc aga tca aaa act tca gcc agg tac        672
Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr           
    210                 215                 220                           

atg gag ctg gtc ggg tgg ctc gtg gac aag ggg att acc tcg gag aag        720
Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys           
225                 230                 235                 240           

cag tgg atc cag gag gac cag gcc tca tac atc tcc ttc aat gcg gcc        768
Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala           
                245                 250                 255               

tcc aac tcg cgg tcc caa atc aag gct gcc ttg gac aat gcg gga aag        816
Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys           
            260                 265                 270                   

att atg agc ctg act aaa acc gcc ccc gac tac ctg gtg ggc cag cag        864
Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln           
        275                 280                 285                       

ccc gtg gag gac att tcc agc aat cgg att tat aaa att ttg gaa cta        912
Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu           
    290                 295                 300                           

agc ggg tac gat ccc caa tat gcg gct tcc gtc ttt ctg gga tgg gcc        960
Ser Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala           
305                 310                 315                 320           

acg aaa aag ttc ggc aag agg aac acc atc tgg ctg ttt ggg cct gca       1008
Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala           
                325                 330                 335               

act acc ggg aag acc aac atc gcg gag gcc ata gcc cac act gtg ccc       1056
Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro           
            340                 345                 350                   

ttc tac ggg tgc gta aac tgg acc aat gag aac ttt ccc ttc aac gac       1104
Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp           
        355                 360                 365                       

tgt gtc gac aag atg gtg atc tgg tgg gag gag ggg aag atg acc gcc       1152
Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala           
    370                 375                 380                           

aag gtc gtg gag tcg gcc aaa gcc att ctc gga gga agc aag gtg cgc       1200
Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg           
385                 390                 395                 400           

gtg gac cag aaa tgc aag tcc tcg gcc cag ata gac ccg act ccc gtg       1248
Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val           
                405                 410                 415               

atc gtc acc tcc aac acc aac atg tgc gcc gtg att gac ggg aac tca       1296
Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser           
            420                 425                 430                   

acg acc ttc gaa cac cag cag ccg ttg caa gac cgg atg ttc aaa ttt       1344
Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe           
        435                 440                 445                       

gaa ctc acc cgc cgt ctg gat cat gac ttt ggg aag gtc acc aag cag       1392
Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln           
    450                 455                 460                           

gaa gtc aaa gac ttt ttc cgg tgg gca aag gat cac gtg gtt gag gtg       1440
Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val           
465                 470                 475                 480           

gag cat gaa ttc tac gtc aaa aag ggt gga gcc aag taa                   1479
Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys                           
                485                 490                                   


<210>  20
<211>  492
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  20

Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp 
1               5                   10                  15      


Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu 
            20                  25                  30          


Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile 
        35                  40                  45              


Glu Gln Ala Pro Leu Thr Val Ala Glu Lys Leu Gln Arg Asp Phe Leu 
    50                  55                  60                  


Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Tyr Val 
65                  70                  75                  80  


Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu 
                85                  90                  95      


Thr Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile 
            100                 105                 110         


Arg Glu Lys Leu Ile Gln Arg Phe Tyr Arg Gly Ile Glu Pro Thr Leu 
        115                 120                 125             


Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly 
    130                 135                 140                 


Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys 
145                 150                 155                 160 


Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu 
                165                 170                 175     


Ser Ala Cys Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His 
            180                 185                 190         


Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn 
        195                 200                 205             


Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr 
    210                 215                 220                 


Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys 
225                 230                 235                 240 


Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala 
                245                 250                 255     


Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys 
            260                 265                 270         


Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln 
        275                 280                 285             


Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu 
    290                 295                 300                 


Ser Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala 
305                 310                 315                 320 


Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala 
                325                 330                 335     


Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro 
            340                 345                 350         


Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp 
        355                 360                 365             


Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala 
    370                 375                 380                 


Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg 
385                 390                 395                 400 


Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val 
                405                 410                 415     


Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser 
            420                 425                 430         


Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe 
        435                 440                 445             


Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln 
    450                 455                 460                 


Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val 
465                 470                 475                 480 


Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys 
                485                 490         


<210>  21
<211>  1479
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pVD220 variant of Rep78 from AAV2


<220>
<221>  CDS
<222>  (1)..(1479)

<400>  21
acg gcg ggg ttc tac gag att gtg att aag gtc ccc agc gac ctt gac         48
Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp           
1               5                   10                  15                

gag cat ctg ccc ggc att tct gac agc ttt gtg aac tgg gtg gcc gag         96
Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu           
            20                  25                  30                    

aag gaa tgg gag ttg ccg cca gat tct gac atg gat ctg aat ctg att        144
Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile           
        35                  40                  45                        

gag cag gca ccc ctg acc gtg gcc ggg aag ctg cag cgc gac ttt ctg        192
Glu Gln Ala Pro Leu Thr Val Ala Gly Lys Leu Gln Arg Asp Phe Leu           
    50                  55                  60                            

acg gaa tgg cgc cgt gtg agt aag gcc ccg gag gcc ctt ttc ttt gtg        240
Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Phe Val           
65                  70                  75                  80            

caa ttt gag aag gga gag agc tac ttc cac atg cac gtg ctc gtg gaa        288
Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu           
                85                  90                  95                

ccc acc ggg gtg aaa tcc atg gtt ttg gga cgt ttc ctg agt cag att        336
Pro Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile           
            100                 105                 110                   

cgc gaa aaa ctg att cag aga att tac cgc ggg atc gag ccg act ttg        384
Arg Glu Lys Leu Ile Gln Arg Ile Tyr Arg Gly Ile Glu Pro Thr Leu           
        115                 120                 125                       

cca aac tgg ttc gcg gtc aca aag acc aga aat ggc gcc gga ggc ggg        432
Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly           
    130                 135                 140                           

aac aag gtg gtg gat gag tgc tac atc ccc aat tac ttg ctc ccc aaa        480
Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys           
145                 150                 155                 160           

acc cag cct gag ctc cag tgg gcg tgg act aat atg gaa cag tat tta        528
Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu           
                165                 170                 175               

agc gcc cgt ttg aat ctc acg gag cgt aaa cgg ttg gtg gcg cag cat        576
Ser Ala Arg Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His           
            180                 185                 190                   

ctg acg cac gtg tcg cag acg cag gag cag aac aaa gag aat cag aat        624
Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn           
        195                 200                 205                       

ccc aat tct gat gcg cct gtg atc aga tca aaa act tca gcc agg tac        672
Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr           
    210                 215                 220                           

atg gag ctg gtc ggg tgg ctc gtg gac aag ggg att acc tcg gag aag        720
Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys           
225                 230                 235                 240           

cag tgg atc cag gag gac cag gcc tca tac atc tcc ttc aat gcg gcc        768
Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala           
                245                 250                 255               

tcc aac tcg cgg tcc caa atc aag gct gcc ttg gac aat gcg gga aag        816
Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys           
            260                 265                 270                   

att atg agc ctg act aaa acc gcc ccc gac tac ctg gtg ggc cag cag        864
Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln           
        275                 280                 285                       

ccc gtg gag gac att tcc agc aat cgg att tat aaa att ttg gaa cta        912
Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu           
    290                 295                 300                           

aac ggg tac gat ccc caa tat gcg gct tcc gtc ttt ctg gga tgg gcc        960
Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala           
305                 310                 315                 320           

acg aaa aag ttc ggc aag agg aac acc atc tgg ctg ttt ggg cct gca       1008
Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala           
                325                 330                 335               

act acc ggg aag acc aac atc gcg gag gcc ata gcc cac act gtg ccc       1056
Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro           
            340                 345                 350                   

ttc tac ggg tgc gta aac tgg acc aat gag aac ttt ccc ttc aac gac       1104
Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp           
        355                 360                 365                       

tgt gtc gac aag atg gtg atc tgg tgg gag gag ggg aag atg acc gcc       1152
Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala           
    370                 375                 380                           

aag gtc gtg gag tcg gcc aaa gcc att ctc gga gga agc aag gtg cgc       1200
Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg           
385                 390                 395                 400           

gtg gac cag aaa tgc aag tcc tcg gcc cag ata gac ccg act ccc gtg       1248
Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val           
                405                 410                 415               

atc gtc acc tcc aac acc aac atg tgc gcc gtg att gac ggg aac tca       1296
Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser           
            420                 425                 430                   

acg acc ttc gaa cac cag cag ccg ttg caa gac cgg atg ttc aaa ttt       1344
Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe           
        435                 440                 445                       

gaa ctc acc cgc cgt ctg gat cat gac ttt ggg aag gtc acc aag cag       1392
Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln           
    450                 455                 460                           

gaa gtc aaa gac ttt ttc cgg tgg gca aag gat cac gtg gtt gag gtg       1440
Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val           
465                 470                 475                 480           

gag cat gaa ttc tac gtc aaa aag ggt gga gcc aag taa                   1479
Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys                           
                485                 490                                   


<210>  22
<211>  492
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  22

Thr Ala Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp 
1               5                   10                  15      


Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu 
            20                  25                  30          


Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile 
        35                  40                  45              


Glu Gln Ala Pro Leu Thr Val Ala Gly Lys Leu Gln Arg Asp Phe Leu 
    50                  55                  60                  


Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Phe Val 
65                  70                  75                  80  


Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu 
                85                  90                  95      


Pro Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile 
            100                 105                 110         


Arg Glu Lys Leu Ile Gln Arg Ile Tyr Arg Gly Ile Glu Pro Thr Leu 
        115                 120                 125             


Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly 
    130                 135                 140                 


Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys 
145                 150                 155                 160 


Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu 
                165                 170                 175     


Ser Ala Arg Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His 
            180                 185                 190         


Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn 
        195                 200                 205             


Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr 
    210                 215                 220                 


Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys 
225                 230                 235                 240 


Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala 
                245                 250                 255     


Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys 
            260                 265                 270         


Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln 
        275                 280                 285             


Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu 
    290                 295                 300                 


Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala 
305                 310                 315                 320 


Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala 
                325                 330                 335     


Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro 
            340                 345                 350         


Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp 
        355                 360                 365             


Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala 
    370                 375                 380                 


Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg 
385                 390                 395                 400 


Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val 
                405                 410                 415     


Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser 
            420                 425                 430         


Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe 
        435                 440                 445             


Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln 
    450                 455                 460                 


Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val 
465                 470                 475                 480 


Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys 
                485                 490         


