LISTADO DE SECUENCIAS 


<110> Consejo Superior de Investigaciones Cientficas (CSIC)y Universidad Politcnica de Valencia 


<120> Vector para la coexpresin de varias protenas heterlogas en cantidades equimolares 


<130> ES1641.687 


<160> 24 


<170> PatentIn version 3.5 


<210>  1 
<211>  11 
<212>  PRT 
<213>  Tobacco etch virus 

<400>  1
Met Ser Glu Leu Val Tyr Ser Gln Gly Glu Lys 
1               5                   10 


<210>  2 
<211>  11 
<212>  PRT 
<213>  Tobacco etch virus 

<400>  2 
Thr Thr Glu Asn Leu Tyr Phe Gln Ser Gly Thr 
1               5                   10 


<210>  3 
<211>  244 
<212>  PRT 
<213>  Artificial Sequence 

<220> 
<223>  Protena mCherry marcada con la etiqueta FLAG y con mutaciones silenciosas 

<400>  3 
Asp Tyr Lys Asp Asp Asp Asp Lys Met Val Ser Lys Gly Glu Glu Asp 
1               5                   10                  15 

Asn Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His Met Glu 
            20                  25                  30         

Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu Gly 
        35                  40                  45             

Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys Gly 
    50                  55                  60                 
 
Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met Tyr 
65                  70                  75                  80 
 
Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr Leu 
                85                  90                  95     
 
Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn Phe 
            100                 105                 110        
 
Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln Asp 
        115                 120                 125            
 
Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro Ser 
    130                 135                 140                
 
Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser Ser 
145                 150                 155                 160
 
Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys Gln 
                165                 170                 175    

Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys Thr 
            180                 185                 190        
 
Thr Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn Val 
        195                 200                 205            

Asn Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile Val 
    210                 215                 220                
 
Glu Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly Gly Met Asp 
225                 230                 235                 240
 
Glu Leu Tyr Lys 


<210>  4 
<211>  248 
<212>  PRT 
<213>  Artificial Sequence 

<220>  
<223>  Protena Venus marcada con la etiqueta HA 

<400>  4 
Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Met Val Ser Lys Gly Glu Glu 
1               5                   10                  15 

Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val 
            20                  25                  30         

Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr 
        35                  40                  45             

Tyr Gly Lys Leu Thr Leu Lys Leu Ile Cys Thr Thr Gly Lys Leu Pro 
    50                  55                  60                  

Val Pro Trp Pro Thr Leu Val Thr Thr Leu Gly Tyr Gly Leu Gln Cys 
65                  70                  75                  80  

Phe Ala Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser 
                85                  90                  95 

Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp 
            100                 105                 110         

Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr 
        115                 120                 125 

Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly 
    130                 135                 140                 

Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val 
145                 150                 155                 160 

Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys 
                165                 170                 175 

Ile Arg His Asn Ile Glu Asp Gly Gly Val Gln Leu Ala Asp His Tyr 
            180                 185                 190         

Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn 
        195                 200                 205            

His Tyr Leu Ser Tyr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys 
    210                 215                 220                 

Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr 
225                 230                 235                 240 

Leu Gly Met Asp Glu Leu Tyr Lys 
                245 


<210>  5 
<211>  243 
<212>  PRT 
<213>  Artificial Sequence 

<220>  
<223>  Protena mTagBFP marcada con la etiqueta c-Myc 

<400>  5 
Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Met Ser Glu Leu Ile Lys 
1               5                   10                  15 

Glu Asn Met His Met Lys Leu Tyr Met Glu Gly Thr Val Asp Asn His 
            20                  25                  30 

His Phe Lys Cys Thr Ser Glu Gly Glu Gly Lys Pro Tyr Glu Gly Thr 
        35                  40                  45             

Gln Thr Met Arg Ile Lys Val Val Glu Gly Gly Pro Leu Pro Phe Ala 
    50                  55                  60              

Phe Asp Ile Leu Ala Thr Ser Phe Leu Tyr Gly Ser Lys Thr Phe Ile 
65                  70                  75                  80 

Asn His Thr Gln Gly Ile Pro Asp Phe Phe Lys Gln Ser Phe Pro Glu 
                85                  90                  95 

Gly Phe Thr Trp Glu Arg Val Thr Thr Tyr Glu Asp Gly Gly Val Leu 
            100                 105                 110 

Thr Ala Thr Gln Asp Thr Ser Leu Gln Asp Gly Cys Leu Ile Tyr Asn 
        115                 120                 125 

Val Lys Ile Arg Gly Val Asn Phe Thr Ser Asn Gly Pro Val Met Gln 
    130                 135                 140 

Lys Lys Thr Leu Gly Trp Glu Ala Phe Thr Glu Thr Leu Tyr Pro Ala 
145                 150                 155                 160 

Asp Gly Gly Leu Glu Gly Arg Asn Asp Met Ala Leu Lys Leu Val Gly 
                165                 170                 175 

Gly Ser His Leu Ile Ala Asn Ile Lys Thr Thr Tyr Arg Ser Lys Lys 
            180                 185                 190 

Pro Ala Lys Asn Leu Lys Met Pro Gly Val Tyr Tyr Val Asp Tyr Arg 
        195                 200                 205 

Leu Glu Arg Ile Lys Glu Ala Asn Asn Glu Thr Tyr Val Glu Gln His 
    210                 215                 220 

Glu Val Ala Val Ala Arg Tyr Cys Asp Leu Pro Ser Lys Leu Gly His 
225                 230                 235                 240 

Lys Leu Asn 


<210>  6 
<211>  60 
<212>  DNA 
<213>  Artificial Sequence 

<220> 
<223>  Cebador PI para amplificar el cDNA de mCherry a partir de pmCherry 

<400>  6 
ggcgggtctc ggaaggacta caaggacgac gatgacaaaa tggttagcaa aggcgaggag   60 


<210>  7 
<211>  35 
<212>  DNA 
<213>  Artificial Sequence 

<220>  
<223>  Cebador PII para amplificar el cDNA de mCherry a partir de pmCherry 

<400>  7 

ggcgggtctc gtcgtcttat acagctcatc catgc                              35 


<210>  8 
<211>  63 
<212>  DNA 
<213>  Artificial Sequence 

<220>  
<223>  Cebador PIII para amplificar el cDNA de Venus a partir de pDH51-GW-YFP 

<400>  8 
ggcgggtctc ggaagtaccc atacgatgtt ccagattacg ctatggtgag caagggcgag   60 
gag                                                                 63 


<210>  9 
<211>  35 
<212>  DNA 
<213>  Artificial Sequence 

<220>  
<223>  Cebador PIV para amplificar el cDNA de Venus a partir de pDH51-GW-YFP 

<400>  9 
ggcgggtctc gtcgtcttgt acagctcgtc catgc                              35 


<210>  10 
<211>  66 
<212>  DNA 
<213>  Artificial Sequence 

<220>  
<223>  Cebador PV para amplificar el cDNA de mTagBFP a partir de pmTagBFP 

<400>  10 
ggcgggtctc ggaaggaaca aaaacttatt tctgaagaag atctgatgag cgagctgatt   60  
aaggag                                                              66 


<210>  11 
<211>  37 
<212>  DNA 
<213>  Artificial Sequence 

<220>  
<223>  Cebador PVI para amplificar el cDNA de mTagBFP a partir de pmTagBFP 

<400>  11 
ggcgggtctc gtcgtattaa gcttgtgccc cagtttg                            37 


<210>  12 
<211>  33 
<212>  DNA 
<213>  Artificial Sequence 

<220>  
<223>  Cebador PVII para amplificar pTEV89 

<400>  12 
ggcgggtctc gcttctcccc ttgcgagtac acc                                33 


<210>  13 
<211>  33 
<212>  DNA 
<213>  Artificial Sequence 

<220>  
<223>  Cebador PVIII para amplificar pTEV89 

<400>  13 
ggcgggtctc gacgactgag aatctttatt ttc                                33
 
 
<210>  14 
<211>  64 
<212>  DNA 
<213>  Artificial Sequence 

<220> 
<223>  Cebador PIX para amplificar el cDNA de FLAG-mCherry a partir de pTEV89ANIb-Cherry 

<400>  14 
ggcgggtctc gagtgccgct ttggaagtac aagttttcag tcgtcttata cagctcatcc   60 
atgc                                                                64 
 

<210>  15 
<211>  39 
<212>  DNA 
<213>  Artificial Sequence 

<220> 
<223>  Cebador PX para amplificar el cDNA de HA-Venus a partir de pTEV89ANIb-Venus
 
<400>  15 
ggcgggtctc gcacttaccc atacgatgtt ccagattac                           39 


<210>  16
<211>  64 
<212>  DNA 
<213>  Artificial Sequence 

<220> 
<223>  Cebador PXI para amplificar el cDNA de HA-Venus a partir de pTEV89ANIb-Venus 

<400> 16 
ggcgggtctc gtgtacctga ttgaaagtag aggttctctg tagtcttgta cagctcgtcc    60 
atgc                                                                 64 

<210>  17 
<211>  37 
<212>  DNA 
<213>  Artificial Sequence 

<220> 
<223>  Cebador PXII para amplificar el cDNA de c-Myc-mTagBFP a partir de pTEV89ANIb-Blue 

<400>  17 
ggcgggtctc gtacagaaca aaaacttatt tctgaag                             37 

 
<210>  18 
<211>  32 
<212>  DNA 
<213>  Artificial Sequence 

<220> 
<223>  Cebador PXIII para amplificar el cDNA de la NIb del TEV a partir de pGTEV 

<400>  18 
ggcgatcgat gggggagaag aggaaatggg tc                                   32 


<210>  19 
<211>  40 
<212>  DNA 
<213>  Artificial Sequence 

<220> 
<223>  Cebador PXIV para amplificar el cDNA de la NIb del TEV a partir de pGTEV 

<400>  19 
ggcgggtctc gtcgatcact gaaaataaag attctcagtc                           40 


<210>  20 
<211>  25 
<212>  DNA 
<213>  Artificial Sequence 

<220>  
<223>  Cebador PXV complementario a las posiciones 9.471-9.495 de TEV-DQ986288 
 
<400>  20 
ctcgcactac ataggagaat tagac                                           25 


<210>  21 
<211>  25 
<212>  DNA 
<213>  Artificial Sequence 

<220> 
<223>  Cebador PXVI homlogo a las posiciones 8.518-8.542 de TEV-DQ986288 

<400>  21 
ctcgcactac ataggagaat tagac                                           25 


<210>  22 
<211>  25 
<212>  DNA 
<213>  Artificial Sequence 

<220> 
<223>  Cebador PXVII complementario a las posiciones 9.289-9.306 de TEV-DQ986288 

<400>  22 
ctcgcactac ataggagaat tagac                                           25 


<210>  23 
<211>  25 
<212>  DNA 
<213>  Artificial Sequence 

<220>  
<223>  Cebador PXVIII homlogo a las posiciones 6.506-6.530 de TEV-DQ986288 

<400>  23 
ctcgcactac ataggagaat tagac                                           25 


<210>  24 
<211>  25 
<212>  DNA 
<213>  Artificial Sequence 

<220>  
<223>  Cebador PXIX complementario a las posiciones 8.500-8.526 de TEV-DQ986288
 
<400>  24 
ctcgcactac ataggagaat tagac                                           25 

