                         SEQUENCE LISTING

<110>  The Salk Institute for Biological Studies
       Joint Center for Biosciences
       Choe, Senyon
       Klammt, Christian
       Kwiatkowski, Witek
       Maslennikov, Innokentiy
 
<120>  Methods and Compositions for NMR Spectroscopic Analysis Using 
       Isotopic Labeling Schemes

<130>  027731-002710PC

<150>  US 61/311,191
<151>  2010-03-05

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide of transmembrane domain of E. coli protein 
       ArcB with Protein Data Bank entry 2ksd

<400>  1

Met Lys Gln Ile Arg Leu Leu Ala Gln Tyr Tyr Val Asp Leu Met Met 
1               5                   10                  15      


Lys Leu Gly Leu Val Arg Phe Ser Met Leu Leu Ala Leu Ala Leu Val 
            20                  25                  30          


Val Leu Ala Ile Val Val Gln Met Ala Val Thr Met Val Leu His Gly 
        35                  40                  45              


Gln Val Glu Ser Ile Asp Val Ile Arg Ser Ile Phe Phe Gly Leu Leu 
    50                  55                  60                  


Ile Thr Pro Trp Ala Val Tyr Phe Leu Ser Val Val Val Glu Gln Leu 
65                  70                  75                  80  


Glu Glu Ser Arg Gln Arg Leu Ser Arg Leu Val Gln Lys Leu Glu Glu 
                85                  90                  95      


Met Arg Glu Arg Asp Leu Ser Leu Asn Val Gln Leu Lys Asp Asn Ile 
            100                 105                 110         


Ala Gln Leu 
        115 


<210>  2
<211>  186
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide of transmembrane domain of E. coli protein 
       QseC with Protein Data Bank entry 2kse

<400>  2

Met Gly Lys Phe Thr Gln Arg Leu Ser Leu Arg Val Arg Leu Thr Leu 
1               5                   10                  15      


Ile Phe Leu Ile Leu Ala Ser Val Thr Trp Leu Leu Ser Ser Phe Val 
            20                  25                  30          


Ala Trp Lys Gln Thr Thr Asp Asn Val Asp Glu Leu Phe Asp Thr Gln 
        35                  40                  45              


Leu Met Leu Phe Ala Lys Arg Leu Ser Thr Leu Asp Leu Asn Glu Ile 
    50                  55                  60                  


Asn Ala Ala Asp Arg Met Ala Gln Thr Pro Asn Arg Leu Lys His Gly 
65                  70                  75                  80  


His Val Asp Asp Asp Ala Leu Thr Phe Ala Ile Phe Thr His Asp Gly 
                85                  90                  95      


Arg Met Val Leu Asn Asp Gly Asp Asn Gly Glu Asp Ile Pro Tyr Ser 
            100                 105                 110         


Tyr Gln Arg Glu Gly Phe Ala Asp Gly Gln Leu Val Gly Glu Asp Asp 
        115                 120                 125             


Pro Trp Arg Phe Val Trp Met Thr Ser Pro Asp Gly Lys Tyr Arg Ile 
    130                 135                 140                 


Val Val Gly Gln Glu Trp Glu Tyr Arg Glu Asp Met Ala Leu Ala Ile 
145                 150                 155                 160 


Val Ala Gly Gln Leu Ile Pro Trp Leu Val Ala Leu Pro Ile Met Leu 
                165                 170                 175     


Ile Ile Met Met Val Leu Leu Gly Arg Glu 
            180                 185     


<210>  3
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide of transmembrane domain of E. coli protein 
       KdpD with Protein Data Bank entry 2ksf

<400>  3

Met Val Gln Ile Gln Gly Ser Val Val Ala Ala Ala Leu Ser Ala Val 
1               5                   10                  15      


Ile Thr Leu Ile Ala Met Gln Trp Leu Met Ala Phe Asp Ala Ala Asn 
            20                  25                  30          


Leu Val Met Leu Tyr Leu Leu Gly Val Val Val Val Ala Leu Phe Tyr 
        35                  40                  45              


Gly Arg Trp Pro Ser Val Val Ala Thr Val Ile Asn Val Val Ser Phe 
    50                  55                  60                  


Asp Leu Phe Phe Ile Ala Pro Arg Gly Thr Leu Ala Val Ser Asp Val 
65                  70                  75                  80  


Gln Tyr Leu Leu Thr Phe Ala Val Met Leu Thr Val Gly Leu Val Ile 
                85                  90                  95      


Gly Asn Leu Thr Ala Gly Val Arg Tyr Gln Ala 
            100                 105         


<210>  4
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide of N-terminal tag which includes a His-9 
       tag

<400>  4

Met Lys His His His His His His His His His Gly Gly Leu Glu Ser 
1               5                   10                  15      


Thr Ser Leu Tyr Lys Lys Ala Gly Ser Leu Val Pro Arg Gly Ser Gly 
            20                  25                  30          


Ser 
    


<210>  5
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide of transmembrane domain of E. coli protein 
       ArcB with Protein Data Bank entry 2ksd and N-terminal construct 
       of SEQ ID NO:4

<400>  5

Met Lys His His His His His His His His His Gly Gly Leu Glu Ser 
1               5                   10                  15      


Thr Ser Leu Tyr Lys Lys Ala Gly Ser Leu Val Pro Arg Gly Ser Gly 
            20                  25                  30          


Ser Met Lys Gln Ile Arg Leu Leu Ala Gln Tyr Tyr Val Asp Leu Met 
        35                  40                  45              


Met Lys Leu Gly Leu Val Arg Phe Ser Met Leu Leu Ala Leu Ala Leu 
    50                  55                  60                  


Val Val Leu Ala Ile Val Val Gln Met Ala Val Thr Met Val Leu His 
65                  70                  75                  80  


Gly Gln Val Glu Ser Ile Asp Val Ile Arg Ser Ile Phe Phe Gly Leu 
                85                  90                  95      


Leu Ile Thr Pro Trp Ala Val Tyr Phe Leu Ser Val Val Val Glu Gln 
            100                 105                 110         


Leu Glu Glu Ser Arg Gln Arg Leu Ser Arg Leu Val Gln Lys Leu Glu 
        115                 120                 125             


Glu Met Arg Glu Arg Asp Leu Ser Leu Asn Val Gln Leu Lys Asp Asn 
    130                 135                 140                 


Ile Ala Gln Leu 
145             


