                         SEQUENCE LISTING

<110>  KARANEWSKY, Donald  S
       FOTSING, Joseph R.
       TACHDJIAN, Catherine
       ARELLANO, Melissa
 
<120>  IDENTIFICATION OF HUMAN T2R RECEPTORS THAT 
       RESPOND TO BITTER COMPOUNDS THAT ELICIT THE 
       BITTER TASTE IN COMPOSITIONS, AND THE USE THEREOF 
       IN ASSAYS TO IDENTIFY COMPOUNDS THAT INHIBIT (BLOCK) 
       BITTER TASTE IN COMPOSITIONS AND USE THEREOF

<130>  67824.704003

<150>  12/656,936
<151>  2010-02-19

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  1

Met Asn Gly Thr Glu Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Lys 
1               5                   10                  15      


Thr Gly Val Val Arg Ser Pro Phe Glu Ala Pro Gln Tyr Tyr Leu Ala 
            20                  25                  30          


Glu Pro Trp 
        35  


<210>  2
<211>  374
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic chimeric protein

<400>  2

Met Ala Arg Ser Leu Thr Trp Arg Cys Cys Pro Trp Cys Leu Thr Glu 
1               5                   10                  15      


Asp Glu Lys Ala Ala Ala Arg Val Asp Gln Glu Ile Asn Arg Ile Leu 
            20                  25                  30          


Leu Glu Gln Lys Lys Gln Asp Arg Gly Glu Leu Lys Leu Leu Leu Leu 
        35                  40                  45              


Gly Pro Gly Glu Ser Gly Lys Ser Thr Phe Ile Lys Gln Met Arg Ile 
    50                  55                  60                  


Ile His Gly Ala Gly Tyr Ser Glu Glu Glu Arg Lys Gly Phe Arg Pro 
65                  70                  75                  80  


Leu Val Tyr Gln Asn Ile Phe Val Ser Met Arg Ala Met Ile Glu Ala 
                85                  90                  95      


Met Glu Arg Leu Gln Ile Pro Phe Ser Arg Pro Glu Ser Lys His His 
            100                 105                 110         


Ala Ser Leu Val Met Ser Gln Asp Pro Tyr Lys Val Thr Thr Phe Glu 
        115                 120                 125             


Lys Arg Tyr Ala Ala Ala Met Gln Trp Leu Trp Arg Asp Ala Gly Ile 
    130                 135                 140                 


Arg Ala Cys Tyr Glu Arg Arg Arg Glu Phe His Leu Leu Asp Ser Ala 
145                 150                 155                 160 


Val Tyr Tyr Leu Ser His Leu Glu Arg Ile Thr Glu Glu Gly Tyr Val 
                165                 170                 175     


Pro Thr Ala Gln Asp Val Leu Arg Ser Arg Met Pro Thr Thr Gly Ile 
            180                 185                 190         


Asn Glu Tyr Cys Phe Ser Val Gln Lys Thr Asn Leu Arg Ile Val Asp 
        195                 200                 205             


Val Gly Gly Gln Lys Ser Glu Arg Lys Lys Trp Ile His Cys Phe Glu 
    210                 215                 220                 


Asn Val Ile Ala Leu Ile Tyr Leu Ala Ser Leu Ser Glu Tyr Asp Gln 
225                 230                 235                 240 


Cys Leu Glu Glu Asn Asn Gln Glu Asn Arg Met Lys Glu Ser Leu Ala 
                245                 250                 255     


Leu Phe Gly Thr Ile Leu Glu Leu Pro Trp Phe Lys Ser Thr Ser Val 
            260                 265                 270         


Ile Leu Phe Leu Asn Lys Thr Asp Ile Leu Glu Glu Lys Ile Pro Thr 
        275                 280                 285             


Ser His Leu Ala Thr Tyr Phe Pro Ser Phe Gln Gly Pro Lys Gln Asp 
    290                 295                 300                 


Ala Glu Ala Ala Lys Arg Phe Ile Leu Asp Met Tyr Thr Arg Met Tyr 
305                 310                 315                 320 


Thr Gly Cys Val Asp Gly Pro Glu Gly Ser Asn Leu Lys Lys Glu Asp 
                325                 330                 335     


Lys Glu Ile Tyr Ser His Met Thr Cys Ala Thr Asp Thr Gln Asn Val 
            340                 345                 350         


Lys Phe Val Phe Asp Ala Val Thr Asp Ile Ile Ile Lys Glu Asn Leu 
        355                 360                 365             


Lys Asp Cys Gly Leu Phe 
    370                 


<210>  3
<211>  930
<212>  DNA
<213>  Homo sapiens

<400>  3
atgttcagtc ctgcagataa catctttata atcctaataa ctggagaatt catactagga       60

atattgggga atggatacat tgcactagtc aactggattg actggattaa gaagaaaaag      120

atttccacag ttgactacat ccttaccaat ttagttatcg ccagaatttg tttgatcagt      180

gtaatggttg taaatggcat tgtaatagta ctgaacccag atgtttatac aaaaaataaa      240

caacagatag tcatttttac cttctggaca tttgccaact acttaaatat gtggattacc      300

acctgcctta atgtcttcta ttttctgaag atagccagtt cctctcatcc actttttctc      360

tggctgaagt ggaaaattga tatggtggtg cactggatcc tgctgggatg ctttgccatt      420

tccttgttgg tcagccttat agcagcaata gtactgagtt gtgattatag gtttcatgca      480

attgccaaac ataaaagaaa cattactgaa atgttccatg tgagtaaaat accatacttt      540

gaacccttaa ctctctttaa cctgtttgca attgtcccat ttattgtgtc actgatatca      600

tttttccttt tagtaagatc tttatggaga cataccaagc aaataaaact ctatgctacc      660

ggcagtagag accccagcac agaagttcat gtgagagcca ttaaaactat gacttcattt      720

atcttctttt ttttcctata ctatatttct tctattttga tgacctttag ctatcttatg      780

acaaaataca agttagctgt ggagtttgga gagattgcag caattctcta ccccttgggt      840

cactcactta ttttaattgt tttaaataat aaactgaggc agacatttgt cagaatgctg      900

acatgtagaa aaattgcctg catgatatga                                       930


<210>  4
<211>  309
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Phe Ser Pro Ala Asp Asn Ile Phe Ile Ile Leu Ile Thr Gly Glu 
1               5                   10                  15      


Phe Ile Leu Gly Ile Leu Gly Asn Gly Tyr Ile Ala Leu Val Asn Trp 
            20                  25                  30          


Ile Asp Trp Ile Lys Lys Lys Lys Ile Ser Thr Val Asp Tyr Ile Leu 
        35                  40                  45              


Thr Asn Leu Val Ile Ala Arg Ile Cys Leu Ile Ser Val Met Val Val 
    50                  55                  60                  


Asn Gly Ile Val Ile Val Leu Asn Pro Asp Val Tyr Thr Lys Asn Lys 
65                  70                  75                  80  


Gln Gln Ile Val Ile Phe Thr Phe Trp Thr Phe Ala Asn Tyr Leu Asn 
                85                  90                  95      


Met Trp Ile Thr Thr Cys Leu Asn Val Phe Tyr Phe Leu Lys Ile Ala 
            100                 105                 110         


Ser Ser Ser His Pro Leu Phe Leu Trp Leu Lys Trp Lys Ile Asp Met 
        115                 120                 125             


Val Val His Trp Ile Leu Leu Gly Cys Phe Ala Ile Ser Leu Leu Val 
    130                 135                 140                 


Ser Leu Ile Ala Ala Ile Val Leu Ser Cys Asp Tyr Arg Phe His Ala 
145                 150                 155                 160 


Ile Ala Lys His Lys Arg Asn Ile Thr Glu Met Phe His Val Ser Lys 
                165                 170                 175     


Ile Pro Tyr Phe Glu Pro Leu Thr Leu Phe Asn Leu Phe Ala Ile Val 
            180                 185                 190         


Pro Phe Ile Val Ser Leu Ile Ser Phe Phe Leu Leu Val Arg Ser Leu 
        195                 200                 205             


Trp Arg His Thr Lys Gln Ile Lys Leu Tyr Ala Thr Gly Ser Arg Asp 
    210                 215                 220                 


Pro Ser Thr Glu Val His Val Arg Ala Ile Lys Thr Met Thr Ser Phe 
225                 230                 235                 240 


Ile Phe Phe Phe Phe Leu Tyr Tyr Ile Ser Ser Ile Leu Met Thr Phe 
                245                 250                 255     


Ser Tyr Leu Met Thr Lys Tyr Lys Leu Ala Val Glu Phe Gly Glu Ile 
            260                 265                 270         


Ala Ala Ile Leu Tyr Pro Leu Gly His Ser Leu Ile Leu Ile Val Leu 
        275                 280                 285             


Asn Asn Lys Leu Arg Gln Thr Phe Val Arg Met Leu Thr Cys Arg Lys 
    290                 295                 300                 


Ile Ala Cys Met Ile 
305                 


<210>  5
<211>  954
<212>  DNA
<213>  Homo sapiens

<400>  5
atgggtggtg tcataaagag catatttaca ttcgttttaa ttgtggaatt tataattgga       60

aatttaggaa atagtttcat agcactggtg aactgtattg actgggtcaa gggaagaaag      120

atctcttcgg ttgatcggat cctcactgct ttggcaatct ctcgaattag cctggtttgg      180

ttaatattcg gaagctggtg tgtgtctgtg tttttcccag ctttatttgc cactgaaaaa      240

atgttcagaa tgcttactaa tatctggaca gtgatcaatc attttagtgt ctggttagct      300

acaggcctcg gtacttttta ttttctcaag atagccaatt tttctaactc tatttttctc      360

tacctaaagt ggagagttaa aaaggtggtt ttggtgctgc ttcttgtgac ttcggtcttc      420

ttgtttttaa atattgcact gataaacatc catataaatg ccagtatcaa tggatacaga      480

agaaacaaga cttgcagttc tgattcaagt aactttacac gattttccag tcttattgta      540

ttaaccagca ctgtgttcat tttcataccc tttactttgt ccctggcaat gtttcttctc      600

ctcatcttct ccatgtggaa acatcgcaag aagatgcagc acactgtcaa aatatccgga      660

gacgccagca ccaaagccca cagaggagtt aaaagtgtga tcactttctt cctactctat      720

gccattttct ctctgtcttt tttcatatca gtttggacct ctgaaaggtt ggaggaaaat      780

ctaattattc tttcccaggt gatgggaatg gcttatcctt catgtcactc atgtgttctg      840

attcttggaa acaagaagct gagacaggcc tctctgtcag tgctactgtg gctgaggtac      900

atgttcaaag atggggagcc ctcaggtcac aaagaattta gagaatcatc ttga            954


<210>  6
<211>  317
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Gly Gly Val Ile Lys Ser Ile Phe Thr Phe Val Leu Ile Val Glu 
1               5                   10                  15      


Phe Ile Ile Gly Asn Leu Gly Asn Ser Phe Ile Ala Leu Val Asn Cys 
            20                  25                  30          


Ile Asp Trp Val Lys Gly Arg Lys Ile Ser Ser Val Asp Arg Ile Leu 
        35                  40                  45              


Thr Ala Leu Ala Ile Ser Arg Ile Ser Leu Val Trp Leu Ile Phe Gly 
    50                  55                  60                  


Ser Trp Cys Val Ser Val Phe Phe Pro Ala Leu Phe Ala Thr Glu Lys 
65                  70                  75                  80  


Met Phe Arg Met Leu Thr Asn Ile Trp Thr Val Ile Asn His Phe Ser 
                85                  90                  95      


Val Trp Leu Ala Thr Gly Leu Gly Thr Phe Tyr Phe Leu Lys Ile Ala 
            100                 105                 110         


Asn Phe Ser Asn Ser Ile Phe Leu Tyr Leu Lys Trp Arg Val Lys Lys 
        115                 120                 125             


Val Val Leu Val Leu Leu Leu Val Thr Ser Val Phe Leu Phe Leu Asn 
    130                 135                 140                 


Ile Ala Leu Ile Asn Ile His Ile Asn Ala Ser Ile Asn Gly Tyr Arg 
145                 150                 155                 160 


Arg Asn Lys Thr Cys Ser Ser Asp Ser Ser Asn Phe Thr Arg Phe Ser 
                165                 170                 175     


Ser Leu Ile Val Leu Thr Ser Thr Val Phe Ile Phe Ile Pro Phe Thr 
            180                 185                 190         


Leu Ser Leu Ala Met Phe Leu Leu Leu Ile Phe Ser Met Trp Lys His 
        195                 200                 205             


Arg Lys Lys Met Gln His Thr Val Lys Ile Ser Gly Asp Ala Ser Thr 
    210                 215                 220                 


Lys Ala His Arg Gly Val Lys Ser Val Ile Thr Phe Phe Leu Leu Tyr 
225                 230                 235                 240 


Ala Ile Phe Ser Leu Ser Phe Phe Ile Ser Val Trp Thr Ser Glu Arg 
                245                 250                 255     


Leu Glu Glu Asn Leu Ile Ile Leu Ser Gln Val Met Gly Met Ala Tyr 
            260                 265                 270         


Pro Ser Cys His Ser Cys Val Leu Ile Leu Gly Asn Lys Lys Leu Arg 
        275                 280                 285             


Gln Ala Ser Leu Ser Val Leu Leu Trp Leu Arg Tyr Met Phe Lys Asp 
    290                 295                 300                 


Gly Glu Pro Ser Gly His Lys Glu Phe Arg Glu Ser Ser 
305                 310                 315         


