                         SEQUENCE LISTING

<110>  TcL PHARMA
       INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE 
       (INSERM)
       MARY, Caroline
       POIRIER, Nicolas
       VANHOVE, Bernard
 
<120>  ANTI-CD28 HUMANIZED ANTIBODIES

<130>  MJP/ll - F2173/3 WO

<150>  EP 10290080.0
<151>  2010-02-18

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCD28.3 VH

<400>  1

Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Lys Lys Pro Gly Ala Ser 
1               5                   10                  15      


Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Glu Tyr Ile 
            20                  25                  30          


Ile His Trp Ile Lys Leu Arg Ser Gly Gln Gly Leu Glu Trp Ile Gly 
        35                  40                  45              


Trp Phe Tyr Pro Gly Ser Asn Asp Ile Gln Tyr Asn Ala Gln Phe Lys 
    50                  55                  60                  


Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Val Tyr Met 
65                  70                  75                  80  


Glu Leu Thr Gly Leu Thr Pro Glu Asp Ser Ala Val Tyr Phe Cys Ala 
                85                  90                  95      


Arg Arg Asp Asp Phe Ser Gly Tyr Asp Ala Leu Pro Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ala 
        115                 120 


<210>  2
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCD28.3 VL


<220>
<221>  MISC_FEATURE
<222>  (96)..(96)
<223>  Xaa = Cys, Ala, or Asn

<400>  2

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Thr Asn Glu Asn Ile Tyr Ser Asn 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Asp Gly Lys Ser Pro Gln Leu Leu Ile 
        35                  40                  45              


Tyr Ala Ala Thr His Leu Val Glu Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Gln Tyr Ser Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Gly Asn Tyr Tyr Cys Gln His Phe Trp Gly Thr Pro Xaa 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105             


<210>  3
<211>  750
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Signal-VH-hCH1

<400>  3
atggaatggt gctgggtctt tctcttcctc ctgtcagtaa ctgcaggtgt ccactccaag       60

gtccaactgc agcagtctgg agctgagctg aagaaacccg gggcgtcggt gaaagtctcc      120

tgcaaggcgt ctggttacac cttcactgaa tatattatac actggataaa gctgaggtct      180

ggacagggtc ttgagtggat tgggtggttt taccctggaa gtaatgatat acagtacaat      240

gcgcaattca agggcaaggc cacattgact gcggacaaat cctccagcac cgtctatatg      300

gaacttactg gattgacacc cgaggactct gcggtctatt tttgtgcaag acgcgacgat      360

ttctctggtt acgacgccct tccttactgg ggccaaggga ctctggtcac tgtctctgca      420

gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg      480

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg      540

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca      600

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc      660

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc      720

aaatcttgtg acaaaactca cacatgctaa                                       750


<210>  4
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal-VH-hCH1

<400>  4

Met Glu Trp Cys Trp Val Phe Leu Phe Leu Leu Ser Val Thr Ala Gly 
1               5                   10                  15      


Val His Ser Lys Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Lys Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Glu Tyr Ile Ile His Trp Ile Lys Leu Arg Ser Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Trp Phe Tyr Pro Gly Ser Asn Asp Ile Gln Tyr Asn 
65                  70                  75                  80  


Ala Gln Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Val Tyr Met Glu Leu Thr Gly Leu Thr Pro Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Phe Cys Ala Arg Arg Asp Asp Phe Ser Gly Tyr Asp Ala Leu Pro 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Ala Ser Thr Lys 
    130                 135                 140                 


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
145                 150                 155                 160 


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
                165                 170                 175     


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
            180                 185                 190         


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
        195                 200                 205             


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
    210                 215                 220                 


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
225                 230                 235                 240 


Lys Ser Cys Asp Lys Thr His Thr Cys Ala Ala 
                245                 250     


<210>  5
<211>  705
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Signal-VL-hCkappa

<400>  5
atgagtgtgc ccactcaggt cctggggttg ctgctgctgt ggcttacaga tgccagatgt       60

gacatccaga tgactcagtc tccatcttcc ctatctgcat ctgtgggaga cagggtcacc      120

atcacgtgta aaacaaatga gaatatttac agtaatttag catggtatca gcagaaagac      180

ggaaaatctc ctcagctcct gatctatgct gcaacacact tagtagaggg tgtgccatca      240

aggttcagtg gcagtggatc aggcacacag tattccctca caatcagcag cctgcagcca      300

gaagattttg ggaattatta ctgtcaacac ttttggggta ctccgtgcac gttcggaggg      360

gggaccaagc tggaaataaa acggacggtg gctgcaccat ctgtcttcat cttcccgcca      420

tctgatgagc agttgaaatc tggaactgcc tctgttgtgt gcctgctgaa taacttctat      480

cccagagagg ccaaagtaca gtggaaggtg gataacgccc tccaatcggg taactcccag      540

gagagtgtca cagagcagga cagcaaggac agcacctaca gcctcagcag caccctgacg      600

ctgagcaaag cagactacga gaaacacaaa gtctacgcct gcgaagtcac ccatcagggc      660

ctgagttcgc ccgtcacaaa gagcttcaac aggggagagt gttaa                      705


<210>  6
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal-VL-hCkappa


<220>
<221>  MISC_FEATURE
<222>  (116)..(116)
<223>  Xaa = Cys or Ala

<400>  6

Met Ser Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr 
1               5                   10                  15      


Asp Ala Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
            20                  25                  30          


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Thr Asn Glu Asn 
        35                  40                  45              


Ile Tyr Ser Asn Leu Ala Trp Tyr Gln Gln Lys Asp Gly Lys Ser Pro 
    50                  55                  60                  


Gln Leu Leu Ile Tyr Ala Ala Thr His Leu Val Glu Gly Val Pro Ser 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Gln Tyr Ser Leu Thr Ile Ser 
                85                  90                  95      


Ser Leu Gln Pro Glu Asp Phe Gly Asn Tyr Tyr Cys Gln His Phe Trp 
            100                 105                 110         


Gly Thr Pro Xaa Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
        115                 120                 125             


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
    130                 135                 140                 


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
145                 150                 155                 160 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
                165                 170                 175     


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
            180                 185                 190         


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
        195                 200                 205             


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
    210                 215                 220                 


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 


<210>  7
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  7

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  8
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  8

Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10  


<210>  9
<211>  1107
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hVHCD28.3-short hingegamma1-hgamma4CH2CH3

<400>  9
atggaatggt gctgggtgtt cctgttcctg ctgtccgtga ccgctggcgt gcactccaag       60

caggtgcagc tgcagcagtc tggcgccgag ctgaagaagc ctggcgcctc cgtcaaggtg      120

tcctgcaagg cctccggcta caccttcacc gagtacatca tccactggat caagctgaga      180

tccggccagg gcctggaatg gatcggctgg ttctaccctg gctccaacga catccagtac      240

aacgcccagt tcaagggcaa ggccaccctg accgccgaca agtcctcctc caccgtgtac      300

atggaactga ccggcctgac ccctgaggac tccgccgtgt acttctgcgc caggcgggac      360

gacttctctg gctacgacgc cctgccttat tggggccagg gcaccctggt gaccgtgtcc      420

gccgacaaaa ctcacacatg cccaccgtgc ccagcacctg agttcctggg gggaccatca      480

gtcttcctgt tccccccaaa acccaaggac actctcatga tctcccggac ccctgaggtc      540

acgtgcgtgg tggtggacgt gagccaggaa gaccccgagg tccagttcaa ctggtacgtg      600

gatggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagtt caacagcacg      660

taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaacgg caaggagtac      720

aagtgcaagg tctccaacaa aggcctcccg tcctccatcg agaaaaccat ctccaaagcc      780

aaagggcagc cccgagagcc acaggtgtac accctgcccc catcccagga ggagatgacc      840

aagaaccagg tcagcctgac ctgcctggtc aaaggcttct accccagcga catcgccgtg      900

gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac      960

tccgacggct ccttcttcct ctacagcagg ctcaccgtgg acaagagcag gtggcaggag     1020

gggaatgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacacagaag     1080

agcctctccc tgtctctggg taaatga                                         1107


<210>  10
<211>  368
<212>  PRT
<213>  Artificial

<220>
<223>  hVHCD28.3-short hingegamma1-hgamma4CH2CH3

<400>  10

Met Glu Trp Cys Trp Val Phe Leu Phe Leu Leu Ser Val Thr Ala Gly 
1               5                   10                  15      


Val His Ser Lys Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Lys 
            20                  25                  30          


Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr 
        35                  40                  45              


Phe Thr Glu Tyr Ile Ile His Trp Ile Lys Leu Arg Ser Gly Gln Gly 
    50                  55                  60                  


Leu Glu Trp Ile Gly Trp Phe Tyr Pro Gly Ser Asn Asp Ile Gln Tyr 
65                  70                  75                  80  


Asn Ala Gln Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser 
                85                  90                  95      


Ser Thr Val Tyr Met Glu Leu Thr Gly Leu Thr Pro Glu Asp Ser Ala 
            100                 105                 110         


Val Tyr Phe Cys Ala Arg Arg Asp Asp Phe Ser Gly Tyr Asp Ala Leu 
        115                 120                 125             


Pro Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Asp Lys Thr 
    130                 135                 140                 


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser 
145                 150                 155                 160 


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
                165                 170                 175     


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro 
            180                 185                 190         


Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
        195                 200                 205             


Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val 
    210                 215                 220                 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
225                 230                 235                 240 


Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr 
                245                 250                 255     


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
            260                 265                 270         


Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
        275                 280                 285             


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
    290                 295                 300                 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
305                 310                 315                 320 


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser 
                325                 330                 335     


Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
            340                 345                 350         


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
        355                 360                 365             


<210>  11
<211>  1068
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hVLCD28.3-short hingegamma1-hgamma4CH2CH3

<400>  11
atgtccgtgc ctacccaggt gctgggactg ctgctgctgt ggctgaccga cgccagatgc       60

gacatccaga tgacccagtc cccctcctcc ctgtctgcct ccgtgggcga ccgggtgacc      120

atcacctgta agaccaacga gaacatctac tccaacctgg cctggtatca gcagaaggac      180

ggcaagtccc ctcagctgct gatctacgcc gccacccatc tggtggaggg cgtgccctct      240

agattctccg gctccggctc tggcacccag tactccctga ccatcagctc cctgcagcct      300

gaggacttcg gcaactacta ctgccagcac ttctggggca ccccttgtac cttcggcgga      360

ggcaccaagc tggaaatcaa gcgggacaaa actcacacat gcccaccgtg cccagcacct      420

gagttcctgg ggggaccatc agtcttcctg ttccccccaa aacccaagga cactctcatg      480

atctcccgga cccctgaggt cacgtgcgtg gtggtggacg tgagccagga agaccccgag      540

gtccagttca actggtacgt ggatggcgtg gaggtgcata atgccaagac aaagccgcgg      600

gaggagcagt tcaacagcac gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac      660

tggctgaacg gcaaggagta caagtgcaag gtctccaaca aaggcctccc gtcctccatc      720

gagaaaacca tctccaaagc caaagggcag ccccgagagc cacaggtgta caccctgccc      780

ccatcccagg aggagatgac caagaaccag gtcagcctga cctgcctggt caaaggcttc      840

taccccagcg acatcgccgt ggagtgggag agcaatgggc agccggagaa caactacaag      900

accacgcctc ccgtgctgga ctccgacggc tccttcttcc tctacagcag gctcaccgtg      960

gacaagagca ggtggcagga ggggaatgtc ttctcatgct ccgtgatgca tgaggctctg     1020

cacaaccact acacacagaa gagcctctcc ctgtctctgg gtaaatga                  1068


<210>  12
<211>  355
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hVLCD28.3-short hingegamma1-hgamma4CH2CH3

<400>  12

Met Ser Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr 
1               5                   10                  15      


Asp Ala Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
            20                  25                  30          


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Thr Asn Glu Asn 
        35                  40                  45              


Ile Tyr Ser Asn Leu Ala Trp Tyr Gln Gln Lys Asp Gly Lys Ser Pro 
    50                  55                  60                  


Gln Leu Leu Ile Tyr Ala Ala Thr His Leu Val Glu Gly Val Pro Ser 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Gln Tyr Ser Leu Thr Ile Ser 
                85                  90                  95      


Ser Leu Gln Pro Glu Asp Phe Gly Asn Tyr Tyr Cys Gln His Phe Trp 
            100                 105                 110         


Gly Thr Pro Cys Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
        115                 120                 125             


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly 
    130                 135                 140                 


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
145                 150                 155                 160 


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln 
                165                 170                 175     


Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
            180                 185                 190         


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr 
        195                 200                 205             


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
    210                 215                 220                 


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile 
225                 230                 235                 240 


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
                245                 250                 255     


Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser 
            260                 265                 270         


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
        275                 280                 285             


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
    290                 295                 300                 


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val 
305                 310                 315                 320 


Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met 
                325                 330                 335     


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
            340                 345                 350         


Leu Gly Lys 
        355 


<210>  13
<211>  1122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hVHCD28.3-full hingegamma1-hgamma4CH2CH3

<400>  13
atggaatggt gctgggtgtt cctgttcctg ctgtccgtga ccgctggcgt gcactccaag       60

caggtgcagc tgcagcagtc tggcgccgag ctgaagaagc ctggcgcctc cgtcaaggtg      120

tcctgcaagg cctccggcta caccttcacc gagtacatca tccactggat caagctgaga      180

tccggccagg gcctggaatg gatcggctgg ttctaccctg gctccaacga catccagtac      240

aacgcccagt tcaagggcaa ggccaccctg accgccgaca agtcctcctc caccgtgtac      300

atggaactga ccggcctgac ccctgaggac tccgccgtgt acttctgcgc caggcgggac      360

gacttctctg gctacgacgc cctgccttat tggggccagg gcaccctggt gaccgtgtcc      420

gccgagccca aatcttgtga caaaactcac acatgcccac cgtgcccagc acctgagttc      480

ctggggggac catcagtctt cctgttcccc ccaaaaccca aggacactct catgatctcc      540

cggacccctg aggtcacgtg cgtggtggtg gacgtgagcc aggaagaccc cgaggtccag      600

ttcaactggt acgtggatgg cgtggaggtg cataatgcca agacaaagcc gcgggaggag      660

cagttcaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca ggactggctg      720

aacggcaagg agtacaagtg caaggtctcc aacaaaggcc tcccgtcctc catcgagaaa      780

accatctcca aagccaaagg gcagccccga gagccacagg tgtacaccct gcccccatcc      840

caggaggaga tgaccaagaa ccaggtcagc ctgacctgcc tggtcaaagg cttctacccc      900

agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta caagaccacg      960

cctcccgtgc tggactccga cggctccttc ttcctctaca gcaggctcac cgtggacaag     1020

agcaggtggc aggaggggaa tgtcttctca tgctccgtga tgcatgaggc tctgcacaac     1080

cactacacac agaagagcct ctccctgtct ctgggtaaat ga                        1122


<210>  14
<211>  373
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hVHCD28.3-full hingegamma1-hgamma4CH2CH3

<400>  14

Met Glu Trp Cys Trp Val Phe Leu Phe Leu Leu Ser Val Thr Ala Gly 
1               5                   10                  15      


Val His Ser Lys Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Lys 
            20                  25                  30          


Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr 
        35                  40                  45              


Phe Thr Glu Tyr Ile Ile His Trp Ile Lys Leu Arg Ser Gly Gln Gly 
    50                  55                  60                  


Leu Glu Trp Ile Gly Trp Phe Tyr Pro Gly Ser Asn Asp Ile Gln Tyr 
65                  70                  75                  80  


Asn Ala Gln Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser 
                85                  90                  95      


Ser Thr Val Tyr Met Glu Leu Thr Gly Leu Thr Pro Glu Asp Ser Ala 
            100                 105                 110         


Val Tyr Phe Cys Ala Arg Arg Asp Asp Phe Ser Gly Tyr Asp Ala Leu 
        115                 120                 125             


Pro Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Glu Pro Lys 
    130                 135                 140                 


Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Phe 
145                 150                 155                 160 


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
                165                 170                 175     


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
            180                 185                 190         


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
        195                 200                 205             


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
    210                 215                 220                 


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
225                 230                 235                 240 


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
                245                 250                 255     


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
            260                 265                 270         


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
        275                 280                 285             


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
    290                 295                 300                 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
305                 310                 315                 320 


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
                325                 330                 335     


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
            340                 345                 350         


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
        355                 360                 365             


Leu Ser Leu Gly Lys 
    370             


<210>  15
<211>  1083
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hVLCD28.3-full hingegamma1-hgamma4CH2CH3

<400>  15
atgtccgtgc ctacccaggt gctgggactg ctgctgctgt ggctgaccga cgccagatgc       60

gacatccaga tgacccagtc cccctcctcc ctgtctgcct ccgtgggcga ccgggtgacc      120

atcacctgta agaccaacga gaacatctac tccaacctgg cctggtatca gcagaaggac      180

ggcaagtccc ctcagctgct gatctacgcc gccacccatc tggtggaggg cgtgccctct      240

agattctccg gctccggctc tggcacccag tactccctga ccatcagctc cctgcagcct      300

gaggacttcg gcaactacta ctgccagcac ttctggggca ccccttgtac cttcggcgga      360

ggcaccaagc tggaaatcaa gcgggagccc aaatcttgtg acaaaactca cacatgccca      420

ccgtgcccag cacctgagtt cctgggggga ccatcagtct tcctgttccc cccaaaaccc      480

aaggacactc tcatgatctc ccggacccct gaggtcacgt gcgtggtggt ggacgtgagc      540

caggaagacc ccgaggtcca gttcaactgg tacgtggatg gcgtggaggt gcataatgcc      600

aagacaaagc cgcgggagga gcagttcaac agcacgtacc gtgtggtcag cgtcctcacc      660

gtcctgcacc aggactggct gaacggcaag gagtacaagt gcaaggtctc caacaaaggc      720

ctcccgtcct ccatcgagaa aaccatctcc aaagccaaag ggcagccccg agagccacag      780

gtgtacaccc tgcccccatc ccaggaggag atgaccaaga accaggtcag cctgacctgc      840

ctggtcaaag gcttctaccc cagcgacatc gccgtggagt gggagagcaa tgggcagccg      900

gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctac      960

agcaggctca ccgtggacaa gagcaggtgg caggagggga atgtcttctc atgctccgtg     1020

atgcatgagg ctctgcacaa ccactacaca cagaagagcc tctccctgtc tctgggtaaa     1080

tga                                                                   1083


<210>  16
<211>  360
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hVLCD28.3-full hingegamma1-hgamma4CH2CH3

<400>  16

Met Ser Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr 
1               5                   10                  15      


Asp Ala Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
            20                  25                  30          


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Thr Asn Glu Asn 
        35                  40                  45              


Ile Tyr Ser Asn Leu Ala Trp Tyr Gln Gln Lys Asp Gly Lys Ser Pro 
    50                  55                  60                  


Gln Leu Leu Ile Tyr Ala Ala Thr His Leu Val Glu Gly Val Pro Ser 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Gln Tyr Ser Leu Thr Ile Ser 
                85                  90                  95      


Ser Leu Gln Pro Glu Asp Phe Gly Asn Tyr Tyr Cys Gln His Phe Trp 
            100                 105                 110         


Gly Thr Pro Cys Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
        115                 120                 125             


Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
    130                 135                 140                 


Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
145                 150                 155                 160 


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
                165                 170                 175     


Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val 
            180                 185                 190         


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
        195                 200                 205             


Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
    210                 215                 220                 


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly 
225                 230                 235                 240 


Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
                245                 250                 255     


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr 
            260                 265                 270         


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
        275                 280                 285             


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
    290                 295                 300                 


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
305                 310                 315                 320 


Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe 
                325                 330                 335     


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
            340                 345                 350         


Ser Leu Ser Leu Ser Leu Gly Lys 
        355                 360 


