                         SEQUENCE LISTING

<110>  Ben-Gurion University of the Negev Research and Development
        Authority
 
<120>  CHEMICAL PREPARATION OF UBIQUITIN THIOESTERS AND MODIFICATIONS 
       THEREOF

<130>  11-078PCT

<140>  PCT/IL2011/000138
<141>  2011-02-09

<150>  US 61/302,559
<151>  2010-02-09

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  76
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  2
<211>  76
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized ubiquitin with the N-terrminus methionine replaced by
       leucine.

<400>  2

Leu Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  3
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide intended for attchment at its C terminal to a 
       latent thioester functionaly (LTF) .

<400>  3

Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile 
1               5                   10                  15      


Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
            20                  25                  30      


<210>  4
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Second fragment in thioester form, intended for native chemical 
       ligation with Ub46-76

<400>  4

Leu Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe 
        35                  40                  45  


<210>  5
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide attached at its C terminus to a latent 
       thioester functionality (LTF).

<400>  5

Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu 
1               5                   10                  15      


Ile Phe Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr 
            20                  25                  30          


Asn Ile Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly 
        35                  40                  45              


Gly 
    


<210>  6
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Second synthetic peptide fragment in thioester form, intended for
       native chemical ligation with Ub28-76

<400>  6

Leu Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys 
            20                  25          


<210>  7
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Third synthetic peptide ubiquitin fragment intended for native 
       chemical ligation together with Ub46-76 and Ub1-27

<400>  7

Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu 
1               5                   10                  15      


Ile Phe 
        


