                         SEQUENCE LISTING

<110>  F. Hoffmann-La Roche AG
 
<120>  IGF-I poly (ethylene glycol) conjugates

<130>  26585 WO

<150>  EP10153275.2
<151>  2010-02-11

<160>  29    

<170>  PatentIn version 3.5

<210>  1
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified trypsine site

<400>  1

Tyr Arg His Ala Ala Gly 
1               5       


<210>  2
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  target peptide

<400>  2

Arg His Ala Lys 
1               


<210>  3
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  displaced part of the tryp site

<400>  3

Arg His Ala Ala Gly 
1               5   


<210>  4
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  remaining peptide

<400>  4

Tyr Arg His Ala Lys 
1               5   


<210>  5
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site 1

<400>  5

Pro Ala Pro Ser Pro 
1               5   


<210>  6
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site 2

<400>  6

Pro Pro Ser Pro 
1               


<210>  7
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site 3

<400>  7

Pro Pro Ala Pro 
1               


<210>  8
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site 4

<400>  8

Pro Pro Thr Pro 
1               


<210>  9
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site 5

<400>  9

Pro Pro Gly Pro 
1               


<210>  10
<211>  103
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IGF-I polypeptide 1

<400>  10

Met His His His His His His Arg Ala Arg Arg Phe Arg Arg His Pro 
1               5                   10                  15      


Arg Pro Pro Gly Pro Glu Thr Leu Cys Gly Ala Glu Leu Val Asp Ala 
            20                  25                  30          


Leu Gln Phe Val Cys Gly Asp Arg Gly Phe Tyr Phe Asn Lys Pro Thr 
        35                  40                  45              


Gly Tyr Gly Ser Ser Ser Arg Arg Ala Pro Gln Thr Gly Ile Val Asp 
    50                  55                  60                  


Glu Cys Cys Phe Arg Ser Cys Asp Leu Arg Arg Leu Glu Met Tyr Cys 
65                  70                  75                  80  


Ala Pro Leu Lys Pro Ala Lys Ser Ala Tyr Arg His Ala Ala Gly Trp 
                85                  90                  95      


Ser His Pro Gln Phe Glu Lys 
            100             


<210>  11
<211>  95
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IGF-I polypeptide 2

<400>  11

Met His His His His His His Arg Ala Arg Arg Phe Arg Arg His Pro 
1               5                   10                  15      


Arg Pro Pro Gly Pro Glu Thr Leu Cys Gly Ala Glu Leu Val Asp Ala 
            20                  25                  30          


Leu Gln Phe Val Cys Gly Asp Arg Gly Phe Tyr Phe Asn Lys Pro Thr 
        35                  40                  45              


Gly Tyr Gly Ser Ser Ser Arg Arg Ala Pro Gln Thr Gly Ile Val Asp 
    50                  55                  60                  


Glu Cys Cys Phe Arg Ser Cys Asp Leu Arg Arg Leu Glu Met Tyr Cys 
65                  70                  75                  80  


Ala Pro Leu Lys Pro Ala Lys Ser Ala Tyr Arg His Ala Ala Gly 
                85                  90                  95  


<210>  12
<211>  95
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IGF-I polypeptide 3

<400>  12

Met His His His His His His Arg Ala Arg Arg Phe Arg Arg His Pro 
1               5                   10                  15      


Arg Pro Pro Gly Pro Glu Thr Leu Cys Gly Ala Glu Leu Val Asp Ala 
            20                  25                  30          


Leu Gln Phe Val Cys Gly Asp Arg Gly Phe Tyr Phe Asn Arg Pro Thr 
        35                  40                  45              


Gly Tyr Gly Ser Ser Ser Arg Arg Ala Pro Gln Thr Gly Ile Val Asp 
    50                  55                  60                  


Glu Cys Cys Phe Arg Ser Cys Asp Leu Arg Arg Leu Glu Met Tyr Cys 
65                  70                  75                  80  


Ala Pro Leu Arg Pro Ala Lys Ser Ala Tyr Arg His Ala Ala Gly 
                85                  90                  95  


<210>  13
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Strep-tag

<400>  13

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  14
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site 6

<400>  14

Ala Pro Arg Pro 
1               


<210>  15
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site 7

<400>  15

Pro Ala Pro Arg Pro 
1               5   


<210>  16
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site 8

<400>  16

Pro Ala Pro Gly Pro 
1               5   


<210>  17
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site 9

<400>  17

Pro Pro Thr Pro Ser Pro 
1               5       


<210>  18
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site 10

<400>  18

Pro Arg Pro Pro Thr Pro 
1               5       


<210>  19
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site 11

<400>  19

Pro Arg Pro Pro Ser Pro 
1               5       


<210>  20
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site 12

<400>  20

Pro Arg Pro Pro Ala Pro 
1               5       


<210>  21
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site 13

<400>  21

Pro Pro Arg Pro Pro Gly Pro 
1               5           


<210>  22
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site 14

<400>  22

Lys Pro Ala Pro Ser Pro 
1               5       


<210>  23
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site 15

<400>  23

Ser Thr Pro Pro Thr Pro 
1               5       


<210>  24
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site 16

<400>  24

Val Ala Pro Pro Ser Pro 
1               5       


<210>  25
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site 17

<400>  25

Ala Pro Arg Pro Pro Gly Pro 
1               5           


<210>  26
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site 18

<400>  26

Pro Ala Pro Arg Pro Pro Gly Pro 
1               5               


<210>  27
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Strep-tag 1

<400>  27

Ala Trp Arg His Pro Gln Phe Gly Gly 
1               5                   


<210>  28
<211>  94
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mono-PEGylated human IGF-I

<400>  28

Met His His His His His His Arg Ala Arg Arg Phe Arg Arg His Pro 
1               5                   10                  15      


Arg Pro Pro Gly Pro Glu Thr Leu Cys Gly Ala Glu Leu Val Asp Ala 
            20                  25                  30          


Leu Gln Phe Val Cys Gly Asp Arg Gly Phe Tyr Phe Asn Lys Pro Thr 
        35                  40                  45              


Gly Tyr Gly Ser Ser Ser Arg Arg Ala Pro Gln Thr Gly Ile Val Asp 
    50                  55                  60                  


Glu Cys Cys Phe Arg Ser Cys Asp Leu Arg Arg Leu Glu Met Tyr Cys 
65                  70                  75                  80  


Ala Pro Leu Lys Pro Ala Lys Ser Ala Tyr Arg His Ala Lys 
                85                  90                  


<210>  29
<211>  75
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-tag cleavaed mono-PEGylated IGF-I

<400>  29

Gly Pro Glu Thr Leu Cys Gly Ala Glu Leu Val Asp Ala Leu Gln Phe 
1               5                   10                  15      


Val Cys Gly Asp Arg Gly Phe Tyr Phe Asn Lys Pro Thr Gly Tyr Gly 
            20                  25                  30          


Ser Ser Ser Arg Arg Ala Pro Gln Thr Gly Ile Val Asp Glu Cys Cys 
        35                  40                  45              


Phe Arg Ser Cys Asp Leu Arg Arg Leu Glu Met Tyr Cys Ala Pro Leu 
    50                  55                  60                  


Lys Pro Ala Lys Ser Ala Tyr Arg His Ala Lys 
65                  70                  75  


