<110>    Samsung Electronics Co. Ltd

<120>    Kit including sequence specific binding protein and method and 
         device for determining nucleotide sequence of target nucleic 
         acid
         

<150>    KR2010-0010500
<151>    2010-02-04

<160>    18

<170>    KopatentIn 1.71

<210>    1
<211>    27
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    YFP-BamHI-F primer


<400>    1
ggatccatgg tgagcaaggg cgaggag                                             27


<210>    2
<211>    27
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    YFP-XhoI-R primer


<400>    2
ctcgagttac ttgtacagct cgtccat                                             27


<210>    3
<211>    7
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    amino acid sequence which specifically recognize "AAT"


<400>    3
Thr Thr Gly Asn Leu Thr Val
  1               5        


<210>    4
<211>    7
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    amino acid sequence which specifically recognize "TAG"


<400>    4
Arg Glu Asp Asn Leu His Thr
  1               5        


<210>    5
<211>    7
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    amino acid sequence which specifically recognize "AAC"


<400>    5
Asp Ser Gly Asn Leu Arg Val
  1               5        


<210>    6
<211>    7
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    amino acid sequence which specifically recognize "TGA"


<400>    6
Gln Ala Gly His Leu Ala Ser
  1               5        


<210>    7
<211>    64
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    amino acid sequence of zinc finger motif which specifically
         recognize "AATTAG"


<400>    7
Leu Glu Pro Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys Ser
  1               5                  10                  15 

Phe Ser Arg Glu Asp Asn Leu His Thr His Gln Arg Thr His Thr Gly
             20                  25                  30 

Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe Ser Thr Thr
         35                  40                  45 

Gly Asn Leu Thr Val His Gln Arg Thr His Thr Gly Lys Lys Thr Ser
     50                  55                  60 




<210>    8
<211>    64
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    amino acid sequence of zinc finger motif which specifically
         recognize "AACTGA"


<400>    8
Leu Glu Pro Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys Ser
  1               5                  10                  15 

Phe Ser Gln Ala Gly His Leu Ala Ser His Gln Arg Thr His Thr Gly
             20                  25                  30 

Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe Ser Asp Ser
         35                  40                  45 

Gly Asn Leu Arg Val His Gln Arg Thr His Thr Gly Lys Lys Thr Ser
     50                  55                  60 




<210>    9
<211>    192
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA sequence(sense) of zinc finger motif which specifically
         recognize "AATTAG"


<400>    9
ctggaaccgg gtgaaaaacc gtacaaatgc ccggaatgcg gtaaatcttt ctctcgtgaa         60

gacaacctgc acacccacca gcgtacccac accggtgaaa aaccgtacaa atgcccggaa        120

tgcggtaaat ctttctctac caccggtaac ctgaccgttc accagcgtac ccacaccggt        180

aaaaaaacct ct                                                            192


<210>    10
<211>    192
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA sequence(antisense) of zinc finger motif which specifically
         recognize "AATTAG"


<400>    10
agaggttttt ttaccggtgt gggtacgctg gtgaacggtc aggttaccgg tggtagagaa         60

agatttaccg cattccgggc atttgtacgg tttttcaccg gtgtgggtac gctggtgggt        120

gtgcaggttg tcttcacgag agaaagattt accgcattcc gggcatttgt acggtttttc        180

acccggttcc ag                                                            192


<210>    11
<211>    192
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA sequence(sense) of zinc finger motif which specifically
         recognize "AACTGA"


<400>    11
ctggaaccgg gtgaaaaacc gtacaaatgc ccggaatgcg gtaaatcttt ctctcaggcg         60

ggtcacctgg cgtctcacca gcgtacccac accggtgaaa aaccgtacaa atgcccggaa        120

tgcggtaaat ctttctctga ctctggtaac ctgcgtgttc accagcgtac ccacaccggt        180

aaaaaaacct ct                                                            192


<210>    12
<211>    192
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA sequence(antisense) of zinc finger motif which specifically
         recognize "AACTGA"


<400>    12
agaggttttt ttaccggtgt gggtacgctg gtgaacacgc aggttaccag agtcagagaa         60

agatttaccg cattccgggc atttgtacgg tttttcaccg gtgtgggtac gctggtgaga        120

cgccaggtga cccgcctgag agaaagattt accgcattcc gggcatttgt acggtttttc        180

acccggttcc ag                                                            192


<210>    13
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    oligonucleotide for EMSA including "AATTAG"(sense strand)


<400>    13
tttttttaat tagttttttt                                                     20


<210>    14
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    oligonucleotide for EMSA including "AATTAG"(antisense strand)


<400>    14
aaaaaaatta atcaaaaaaa                                                     20


<210>    15
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    oligonucleotide for EMSA including "AACTGA"(sense strand)


<400>    15
tttttttaac tgattttttt                                                     20


<210>    16
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    oligonucleotide for EMSA including "AACTGA"(antisense strand)


<400>    16
aaaaaaattg actaaaaaaa                                                     20

