                         SEQUENCE LISTING

<110>  New York University
 
<120>  RECOMBINANT DERIVATIVES OF BOTULINUM NEUROTOXINS ENGINEERED FOR 
       TRAFFICKING STUDIES AND NEURONAL DELIVERY

<130>  29527.0272 (57953/1842)

<140>  PCT/US11/22408
<141>  2011-01-25

<150>  US 61/298,078
<151>  2010-01-25

<160>  49    

<170>  PatentIn version 3.5

<210>  1
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  Botulinum Neurotoxin Light Chain Motif


<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  1

His Glu Xaa Xaa His Xaa Xaa His 
1               5               


<210>  2
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  S6 Peptide Sequence

<400>  2

Gly Asp Ser Leu Ser Trp Leu Leu Arg Leu Leu Asn 
1               5                   10          


<210>  3
<211>  1372
<212>  PRT
<213>  Artificial

<220>
<223>  BoNT/Aadek Neurotoxin

<400>  3

Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      


Ser Tyr Ile Tyr Ala Ala Ala His His His His His His His His His 
            20                  25                  30          


His Thr Arg Glu Asn Leu Tyr Phe Gln Gly Ala Gly Asp Ser Leu Ser 
        35                  40                  45              


Trp Leu Leu Arg Leu Leu Asn Ala Arg Gly Gly Ala Ser Gly Pro Phe 
    50                  55                  60                  


Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly Val Asp Ile 
65                  70                  75                  80  


Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro Val Lys Ala 
                85                  90                  95      


Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg Asp Thr Phe 
            100                 105                 110         


Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu Ala Lys Gln 
        115                 120                 125             


Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr Asp Asn Glu 
    130                 135                 140                 


Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu Arg Ile Tyr 
145                 150                 155                 160 


Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val Arg Gly Ile 
                165                 170                 175     


Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys Val Ile Asp 
            180                 185                 190         


Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr Arg Ser Glu 
        195                 200                 205             


Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile Ile Gln Phe 
    210                 215                 220                 


Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr Arg Asn Gly 
225                 230                 235                 240 


Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe Thr Phe Gly 
                245                 250                 255     


Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu Gly Ala Gly 
            260                 265                 270         


Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Ala Leu Ile His 
        275                 280                 285             


Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn Arg Val Phe 
    290                 295                 300                 


Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu Glu Val Ser 
305                 310                 315                 320 


Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys Phe Ile Asp 
                325                 330                 335     


Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn Lys Phe Lys 
            340                 345                 350         


Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val Gly Thr Thr 
        355                 360                 365             


Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys Tyr Leu Leu 
    370                 375                 380                 


Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu Lys Phe Asp 
385                 390                 395                 400 


Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp Asn Phe Val 
                405                 410                 415     


Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Ala Leu Asn Phe Asp Lys 
            420                 425                 430         


Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr Thr Ile Tyr 
        435                 440                 445             


Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn Phe Asn Gly 
    450                 455                 460                 


Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu Lys Asn Phe 
465                 470                 475                 480 


Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg Gly Ile Ile 
                485                 490                 495     


Thr Ser His Thr Gln Ser Leu Asp Gln Gly Tyr Asn Asp Asp Asp Asp 
            500                 505                 510         


Lys Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe 
        515                 520                 525             


Phe Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu 
    530                 535                 540                 


Glu Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser 
545                 550                 555                 560 


Leu Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu 
                565                 570                 575     


Pro Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln 
            580                 585                 590         


Leu Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr 
        595                 600                 605             


Glu Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe 
    610                 615                 620                 


Glu His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala 
625                 630                 635                 640 


Leu Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val 
                645                 650                 655     


Lys Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val 
            660                 665                 670         


Glu Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr 
        675                 680                 685             


Thr Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro 
    690                 695                 700                 


Ala Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala 
705                 710                 715                 720 


Leu Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile 
                725                 730                 735     


Ala Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn 
            740                 745                 750         


Lys Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn 
        755                 760                 765             


Glu Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala 
    770                 775                 780                 


Lys Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala 
785                 790                 795                 800 


Leu Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr 
                805                 810                 815     


Asn Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp 
            820                 825                 830         


Asp Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn 
        835                 840                 845             


Ile Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser 
    850                 855                 860                 


Met Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu 
865                 870                 875                 880 


Lys Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile 
                885                 890                 895     


Gly Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr 
            900                 905                 910         


Asp Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu 
        915                 920                 925             


Ser Thr Phe Thr Glu Tyr Ile Asn Asn Ile Ile Asn Thr Ser Ile Leu 
    930                 935                 940                 


Asn Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala 
945                 950                 955                 960 


Ser Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys 
                965                 970                 975     


Asn Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile 
            980                 985                 990         


Leu Lys Asn Ala Ile Val Tyr Asn  Ser Met Tyr Glu Asn  Phe Ser Thr 
        995                 1000                 1005             


Ser Phe  Trp Ile Arg Ile Pro  Lys Tyr Phe Asn Ser  Ile Ser Leu 
    1010                 1015                 1020             


Asn Asn  Glu Tyr Thr Ile Ile  Asn Cys Met Glu Asn  Asn Ser Gly 
    1025                 1030                 1035             


Trp Lys  Val Ser Leu Asn Tyr  Gly Glu Ile Ile Trp  Thr Leu Gln 
    1040                 1045                 1050             


Asp Thr  Gln Glu Ile Lys Gln  Arg Val Val Phe Lys  Tyr Ser Gln 
    1055                 1060                 1065             


Met Ile  Asn Ile Ser Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr 
    1070                 1075                 1080             


Ile Thr  Asn Asn Arg Leu Asn  Asn Ser Lys Ile Tyr  Ile Asn Gly 
    1085                 1090                 1095             


Arg Leu  Ile Asp Gln Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His 
    1100                 1105                 1110             


Ala Ser  Asn Asn Ile Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr 
    1115                 1120                 1125             


His Arg  Tyr Ile Trp Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu 
    1130                 1135                 1140             


Leu Asn  Glu Lys Glu Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn 
    1145                 1150                 1155             


Ser Gly  Ile Leu Lys Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp 
    1160                 1165                 1170             


Lys Pro  Tyr Tyr Met Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val 
    1175                 1180                 1185             


Asp Val  Asn Asn Val Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly 
    1190                 1195                 1200             


Pro Arg  Gly Ser Val Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser 
    1205                 1210                 1215             


Leu Tyr  Arg Gly Thr Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly 
    1220                 1225                 1230             


Asn Lys  Asp Asn Ile Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn 
    1235                 1240                 1245             


Val Val  Val Lys Asn Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser 
    1250                 1255                 1260             


Gln Ala  Gly Val Glu Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Asp 
    1265                 1270                 1275             


Val Gly  Asn Leu Ser Gln Val  Val Val Met Lys Ser  Lys Asn Asp 
    1280                 1285                 1290             


Gln Gly  Ile Thr Asn Lys Cys  Lys Met Asn Leu Gln  Asp Asn Asn 
    1295                 1300                 1305             


Gly Asn  Asp Ile Gly Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile 
    1310                 1315                 1320             


Ala Lys  Leu Val Ala Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg 
    1325                 1330                 1335             


Ser Ser  Arg Thr Leu Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp 
    1340                 1345                 1350             


Asp Gly  Trp Gly Glu Arg Pro  Leu Gly Ala Gly Trp  Ser His Pro 
    1355                 1360                 1365             


Gln Phe  Glu Lys 
    1370         


<210>  4
<211>  1373
<212>  PRT
<213>  Artificial

<220>
<223>  BoNT/Aadtev Neurotoxin

<400>  4

Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      


Ser Tyr Ile Tyr Ala Ala Ala His His His His His His His His His 
            20                  25                  30          


His Thr Arg Glu Asn Leu Tyr Phe Gln Gly Ala Gly Asp Ser Leu Ser 
        35                  40                  45              


Trp Leu Leu Arg Leu Leu Asn Ala Arg Gly Gly Ala Ser Gly Pro Phe 
    50                  55                  60                  


Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly Val Asp Ile 
65                  70                  75                  80  


Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro Val Lys Ala 
                85                  90                  95      


Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg Asp Thr Phe 
            100                 105                 110         


Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu Ala Lys Gln 
        115                 120                 125             


Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr Asp Asn Glu 
    130                 135                 140                 


Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu Arg Ile Tyr 
145                 150                 155                 160 


Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val Arg Gly Ile 
                165                 170                 175     


Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys Val Ile Asp 
            180                 185                 190         


Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr Arg Ser Glu 
        195                 200                 205             


Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile Ile Gln Phe 
    210                 215                 220                 


Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr Arg Asn Gly 
225                 230                 235                 240 


Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe Thr Phe Gly 
                245                 250                 255     


Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu Gly Ala Gly 
            260                 265                 270         


Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Ala Leu Ile His 
        275                 280                 285             


Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn Arg Val Phe 
    290                 295                 300                 


Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu Glu Val Ser 
305                 310                 315                 320 


Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys Phe Ile Asp 
                325                 330                 335     


Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn Lys Phe Lys 
            340                 345                 350         


Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val Gly Thr Thr 
        355                 360                 365             


Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys Tyr Leu Leu 
    370                 375                 380                 


Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu Lys Phe Asp 
385                 390                 395                 400 


Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp Asn Phe Val 
                405                 410                 415     


Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Ala Leu Asn Phe Asp Lys 
            420                 425                 430         


Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr Thr Ile Tyr 
        435                 440                 445             


Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn Phe Asn Gly 
    450                 455                 460                 


Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu Lys Asn Phe 
465                 470                 475                 480 


Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg Gly Ile Ile 
                485                 490                 495     


Thr Ser His Thr Gln Ser Leu Asp Gln Gly Gly Glu Asn Leu Tyr Phe 
            500                 505                 510         


Gln Gly Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu 
        515                 520                 525             


Phe Phe Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly 
    530                 535                 540                 


Glu Glu Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile 
545                 550                 555                 560 


Ser Leu Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn 
                565                 570                 575     


Glu Pro Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly 
            580                 585                 590         


Gln Leu Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys 
        595                 600                 605             


Tyr Glu Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu 
    610                 615                 620                 


Phe Glu His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu 
625                 630                 635                 640 


Ala Leu Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr 
                645                 650                 655     


Val Lys Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp 
            660                 665                 670         


Val Glu Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser 
        675                 680                 685             


Thr Thr Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly 
    690                 695                 700                 


Pro Ala Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly 
705                 710                 715                 720 


Ala Leu Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu 
                725                 730                 735     


Ile Ala Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala 
            740                 745                 750         


Asn Lys Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg 
        755                 760                 765             


Asn Glu Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu 
    770                 775                 780                 


Ala Lys Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu 
785                 790                 795                 800 


Ala Leu Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln 
                805                 810                 815     


Tyr Asn Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile 
            820                 825                 830         


Asp Asp Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile 
        835                 840                 845             


Asn Ile Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn 
    850                 855                 860                 


Ser Met Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser 
865                 870                 875                 880 


Leu Lys Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu 
                885                 890                 895     


Ile Gly Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser 
            900                 905                 910         


Thr Asp Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu 
        915                 920                 925             


Leu Ser Thr Phe Thr Glu Tyr Ile Asn Asn Ile Ile Asn Thr Ser Ile 
    930                 935                 940                 


Leu Asn Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr 
945                 950                 955                 960 


Ala Ser Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp 
                965                 970                 975     


Lys Asn Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val 
            980                 985                 990         


Ile Leu Lys Asn Ala Ile Val Tyr  Asn Ser Met Tyr Glu  Asn Phe Ser 
        995                 1000                 1005             


Thr Ser  Phe Trp Ile Arg Ile  Pro Lys Tyr Phe Asn  Ser Ile Ser 
    1010                 1015                 1020             


Leu Asn  Asn Glu Tyr Thr Ile  Ile Asn Cys Met Glu  Asn Asn Ser 
    1025                 1030                 1035             


Gly Trp  Lys Val Ser Leu Asn  Tyr Gly Glu Ile Ile  Trp Thr Leu 
    1040                 1045                 1050             


Gln Asp  Thr Gln Glu Ile Lys  Gln Arg Val Val Phe  Lys Tyr Ser 
    1055                 1060                 1065             


Gln Met  Ile Asn Ile Ser Asp  Tyr Ile Asn Arg Trp  Ile Phe Val 
    1070                 1075                 1080             


Thr Ile  Thr Asn Asn Arg Leu  Asn Asn Ser Lys Ile  Tyr Ile Asn 
    1085                 1090                 1095             


Gly Arg  Leu Ile Asp Gln Lys  Pro Ile Ser Asn Leu  Gly Asn Ile 
    1100                 1105                 1110             


His Ala  Ser Asn Asn Ile Met  Phe Lys Leu Asp Gly  Cys Arg Asp 
    1115                 1120                 1125             


Thr His  Arg Tyr Ile Trp Ile  Lys Tyr Phe Asn Leu  Phe Asp Lys 
    1130                 1135                 1140             


Glu Leu  Asn Glu Lys Glu Ile  Lys Asp Leu Tyr Asp  Asn Gln Ser 
    1145                 1150                 1155             


Asn Ser  Gly Ile Leu Lys Asp  Phe Trp Gly Asp Tyr  Leu Gln Tyr 
    1160                 1165                 1170             


Asp Lys  Pro Tyr Tyr Met Leu  Asn Leu Tyr Asp Pro  Asn Lys Tyr 
    1175                 1180                 1185             


Val Asp  Val Asn Asn Val Gly  Ile Arg Gly Tyr Met  Tyr Leu Lys 
    1190                 1195                 1200             


Gly Pro  Arg Gly Ser Val Met  Thr Thr Asn Ile Tyr  Leu Asn Ser 
    1205                 1210                 1215             


Ser Leu  Tyr Arg Gly Thr Lys  Phe Ile Ile Lys Lys  Tyr Ala Ser 
    1220                 1225                 1230             


Gly Asn  Lys Asp Asn Ile Val  Arg Asn Asn Asp Arg  Val Tyr Ile 
    1235                 1240                 1245             


Asn Val  Val Val Lys Asn Lys  Glu Tyr Arg Leu Ala  Thr Asn Ala 
    1250                 1255                 1260             


Ser Gln  Ala Gly Val Glu Lys  Ile Leu Ser Ala Leu  Glu Ile Pro 
    1265                 1270                 1275             


Asp Val  Gly Asn Leu Ser Gln  Val Val Val Met Lys  Ser Lys Asn 
    1280                 1285                 1290             


Asp Gln  Gly Ile Thr Asn Lys  Cys Lys Met Asn Leu  Gln Asp Asn 
    1295                 1300                 1305             


Asn Gly  Asn Asp Ile Gly Phe  Ile Gly Phe His Gln  Phe Asn Asn 
    1310                 1315                 1320             


Ile Ala  Lys Leu Val Ala Ser  Asn Trp Tyr Asn Arg  Gln Ile Glu 
    1325                 1330                 1335             


Arg Ser  Ser Arg Thr Leu Gly  Cys Ser Trp Glu Phe  Ile Pro Val 
    1340                 1345                 1350             


Asp Asp  Gly Trp Gly Glu Arg  Pro Leu Gly Ala Gly  Trp Ser His 
    1355                 1360                 1365             


Pro Gln  Phe Glu Lys 
    1370             


<210>  5
<211>  993
<212>  PRT
<213>  Artificial

<220>
<223>  Delta LC-Peptide-BoNT/Atev Neurotoxin

<400>  5

Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      


Ser Tyr Ile Tyr Ala Ala Ala His His His His His His His His His 
            20                  25                  30          


His Thr Arg Glu Asn Leu Tyr Phe Gln Gly Ala Gly Asp Ser Leu Ser 
        35                  40                  45              


Trp Leu Leu Arg Leu Leu Asn Ala Arg Gly Gly Ala Ser Gly Gly Thr 
    50                  55                  60                  


Asn Gly Asn Gly Asn Gly Gly Asn Leu Arg Asn Thr Asn Leu Ala Ala 
65                  70                  75                  80  


Asn Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys 
                85                  90                  95      


Leu Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val 
            100                 105                 110         


Arg Gly Ile Ile Thr Ser His Thr Gln Ser Leu Asp Gln Gly Gly Glu 
        115                 120                 125             


Asn Leu Tyr Phe Gln Gly Ala Leu Asn Asp Leu Cys Ile Lys Val Asn 
    130                 135                 140                 


Asn Trp Asp Leu Phe Phe Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp 
145                 150                 155                 160 


Leu Asn Lys Gly Glu Glu Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala 
                165                 170                 175     


Glu Glu Asn Ile Ser Leu Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe 
            180                 185                 190         


Asn Phe Asp Asn Glu Pro Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser 
        195                 200                 205             


Asp Ile Ile Gly Gln Leu Glu Leu Met Pro Asn Ile Glu Arg Phe Pro 
    210                 215                 220                 


Asn Gly Lys Lys Tyr Glu Leu Asp Lys Tyr Thr Met Phe His Tyr Leu 
225                 230                 235                 240 


Arg Ala Gln Glu Phe Glu His Gly Lys Ser Arg Ile Ala Leu Thr Asn 
                245                 250                 255     


Ser Val Asn Glu Ala Leu Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe 
            260                 265                 270         


Ser Ser Asp Tyr Val Lys Lys Val Asn Lys Ala Thr Glu Ala Ala Met 
        275                 280                 285             


Phe Leu Gly Trp Val Glu Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr 
    290                 295                 300                 


Ser Glu Val Ser Thr Thr Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile 
305                 310                 315                 320 


Pro Tyr Ile Gly Pro Ala Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp 
                325                 330                 335     


Asp Phe Val Gly Ala Leu Ile Phe Ser Gly Ala Val Ile Leu Leu Glu 
            340                 345                 350         


Phe Ile Pro Glu Ile Ala Ile Pro Val Leu Gly Thr Phe Ala Leu Val 
        355                 360                 365             


Ser Tyr Ile Ala Asn Lys Val Leu Thr Val Gln Thr Ile Asp Asn Ala 
    370                 375                 380                 


Leu Ser Lys Arg Asn Glu Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val 
385                 390                 395                 400 


Thr Asn Trp Leu Ala Lys Val Asn Thr Gln Ile Asp Leu Ile Arg Lys 
                405                 410                 415     


Lys Met Lys Glu Ala Leu Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile 
            420                 425                 430         


Ile Asn Tyr Gln Tyr Asn Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile 
        435                 440                 445             


Asn Phe Asn Ile Asp Asp Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn 
    450                 455                 460                 


Lys Ala Met Ile Asn Ile Asn Lys Phe Leu Asn Gln Cys Ser Val Ser 
465                 470                 475                 480 


Tyr Leu Met Asn Ser Met Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp 
                485                 490                 495     


Phe Asp Ala Ser Leu Lys Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn 
            500                 505                 510         


Arg Gly Thr Leu Ile Gly Gln Val Asp Arg Leu Lys Asp Lys Val Asn 
        515                 520                 525             


Asn Thr Leu Ser Thr Asp Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp 
    530                 535                 540                 


Asn Gln Arg Leu Leu Ser Thr Phe Thr Glu Tyr Ile Asn Asn Ile Ile 
545                 550                 555                 560 


Asn Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His Leu Ile Asp 
                565                 570                 575     


Leu Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys Val Asn Phe 
            580                 585                 590         


Asp Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser 
        595                 600                 605             


Lys Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr 
    610                 615                 620                 


Glu Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn 
625                 630                 635                 640 


Ser Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn 
                645                 650                 655     


Asn Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr 
            660                 665                 670         


Leu Gln Asp Thr Gln Glu Ile Lys Gln Arg Val Val Phe Lys Tyr Ser 
        675                 680                 685             


Gln Met Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp Ile Phe Val Thr 
    690                 695                 700                 


Ile Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr Ile Asn Gly Arg 
705                 710                 715                 720 


Leu Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn Ile His Ala Ser 
                725                 730                 735     


Asn Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp Thr His Arg Tyr 
            740                 745                 750         


Ile Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu Leu Asn Glu Lys 
        755                 760                 765             


Glu Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser Gly Ile Leu Lys 
    770                 775                 780                 


Asp Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys Pro Tyr Tyr Met Leu 
785                 790                 795                 800 


Asn Leu Tyr Asp Pro Asn Lys Tyr Val Asp Val Asn Asn Val Gly Ile 
                805                 810                 815     


Arg Gly Tyr Met Tyr Leu Lys Gly Pro Arg Gly Ser Val Met Thr Thr 
            820                 825                 830         


Asn Ile Tyr Leu Asn Ser Ser Leu Tyr Arg Gly Thr Lys Phe Ile Ile 
        835                 840                 845             


Lys Lys Tyr Ala Ser Gly Asn Lys Asp Asn Ile Val Arg Asn Asn Asp 
    850                 855                 860                 


Arg Val Tyr Ile Asn Val Val Val Lys Asn Lys Glu Tyr Arg Leu Ala 
865                 870                 875                 880 


Thr Asn Ala Ser Gln Ala Gly Val Glu Lys Ile Leu Ser Ala Leu Glu 
                885                 890                 895     


Ile Pro Asp Val Gly Asn Leu Ser Gln Val Val Val Met Lys Ser Lys 
            900                 905                 910         


Asn Asp Gln Gly Ile Thr Asn Lys Cys Lys Met Asn Leu Gln Asp Asn 
        915                 920                 925             


Asn Gly Asn Asp Ile Gly Phe Ile Gly Phe His Gln Phe Asn Asn Ile 
    930                 935                 940                 


Ala Lys Leu Val Ala Ser Asn Trp Tyr Asn Arg Gln Ile Glu Arg Ser 
945                 950                 955                 960 


Ser Arg Thr Leu Gly Cys Ser Trp Glu Phe Ile Pro Val Asp Asp Gly 
                965                 970                 975     


Trp Gly Glu Arg Pro Leu Gly Ala Gly Trp Ser His Pro Gln Phe Glu 
            980                 985                 990         


Lys 
    


<210>  6
<211>  1230
<212>  PRT
<213>  Artificial

<220>
<223>  Delta LC-GFP-BoNT/Atev Neurotoxin

<400>  6

Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      


Ser Tyr Ile Tyr Ala Ala Ala His His His His His His His His His 
            20                  25                  30          


His Thr Arg Glu Asn Leu Tyr Phe Gln Gly Ala Gly Asp Ser Leu Ser 
        35                  40                  45              


Trp Leu Leu Arg Leu Leu Asn Ala Arg Gly Gly Ala Ser Val Ser Lys 
    50                  55                  60                  


Gly Ala Glu Leu Phe Thr Gly Ile Val Pro Ile Leu Ile Glu Leu Asn 
65                  70                  75                  80  


Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly 
                85                  90                  95      


Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly 
            100                 105                 110         


Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Ser Tyr Gly 
        115                 120                 125             


Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe 
    130                 135                 140                 


Phe Lys Ser Ala Met Pro Glu Gly Tyr Ile Gln Glu Arg Thr Ile Phe 
145                 150                 155                 160 


Phe Glu Asp Asp Gly Asn Tyr Lys Ser Arg Ala Glu Val Lys Phe Glu 
                165                 170                 175     


Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Thr Gly Thr Asp Phe Lys 
            180                 185                 190         


Glu Asp Gly Asn Ile Leu Gly Asn Lys Met Glu Tyr Asn Tyr Asn Ala 
        195                 200                 205             


His Asn Val Tyr Ile Met Thr Asp Lys Ala Lys Asn Gly Ile Lys Val 
    210                 215                 220                 


Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala 
225                 230                 235                 240 


Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu 
                245                 250                 255     


Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro 
            260                 265                 270         


Asn Glu Lys Arg Asp His Met Ile Tyr Phe Gly Phe Val Thr Ala Ala 
        275                 280                 285             


Ala Ile Thr His Gly Met Asp Glu Leu Tyr Lys Gly Thr Asn Gly Asn 
    290                 295                 300                 


Gly Asn Gly Gly Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn Phe Asn 
305                 310                 315                 320 


Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu Lys Asn 
                325                 330                 335     


Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg Gly Ile 
            340                 345                 350         


Ile Thr Ser His Thr Gln Ser Leu Asp Gln Gly Gly Glu Asn Leu Tyr 
        355                 360                 365             


Phe Gln Gly Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp 
    370                 375                 380                 


Leu Phe Phe Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys 
385                 390                 395                 400 


Gly Glu Glu Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn 
                405                 410                 415     


Ile Ser Leu Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp 
            420                 425                 430         


Asn Glu Pro Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile 
        435                 440                 445             


Gly Gln Leu Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys 
    450                 455                 460                 


Lys Tyr Glu Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln 
465                 470                 475                 480 


Glu Phe Glu His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn 
                485                 490                 495     


Glu Ala Leu Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp 
            500                 505                 510         


Tyr Val Lys Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly 
        515                 520                 525             


Trp Val Glu Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val 
    530                 535                 540                 


Ser Thr Thr Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile 
545                 550                 555                 560 


Gly Pro Ala Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val 
                565                 570                 575     


Gly Ala Leu Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro 
            580                 585                 590         


Glu Ile Ala Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile 
        595                 600                 605             


Ala Asn Lys Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys 
    610                 615                 620                 


Arg Asn Glu Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp 
625                 630                 635                 640 


Leu Ala Lys Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys 
                645                 650                 655     


Glu Ala Leu Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr 
            660                 665                 670         


Gln Tyr Asn Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn 
        675                 680                 685             


Ile Asp Asp Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met 
    690                 695                 700                 


Ile Asn Ile Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met 
705                 710                 715                 720 


Asn Ser Met Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala 
                725                 730                 735     


Ser Leu Lys Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr 
            740                 745                 750         


Leu Ile Gly Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu 
        755                 760                 765             


Ser Thr Asp Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg 
    770                 775                 780                 


Leu Leu Ser Thr Phe Thr Glu Tyr Ile Asn Asn Ile Ile Asn Thr Ser 
785                 790                 795                 800 


Ile Leu Asn Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg 
                805                 810                 815     


Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile 
            820                 825                 830         


Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu 
        835                 840                 845             


Val Ile Leu Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe 
    850                 855                 860                 


Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser 
865                 870                 875                 880 


Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly 
                885                 890                 895     


Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp 
            900                 905                 910         


Thr Gln Glu Ile Lys Gln Arg Val Val Phe Lys Tyr Ser Gln Met Ile 
        915                 920                 925             


Asn Ile Ser Asp Tyr Ile Asn Arg Trp Ile Phe Val Thr Ile Thr Asn 
    930                 935                 940                 


Asn Arg Leu Asn Asn Ser Lys Ile Tyr Ile Asn Gly Arg Leu Ile Asp 
945                 950                 955                 960 


Gln Lys Pro Ile Ser Asn Leu Gly Asn Ile His Ala Ser Asn Asn Ile 
                965                 970                 975     


Met Phe Lys Leu Asp Gly Cys Arg Asp Thr His Arg Tyr Ile Trp Ile 
            980                 985                 990         


Lys Tyr Phe Asn Leu Phe Asp Lys  Glu Leu Asn Glu Lys  Glu Ile Lys 
        995                 1000                 1005             


Asp Leu  Tyr Asp Asn Gln Ser  Asn Ser Gly Ile Leu  Lys Asp Phe 
    1010                 1015                 1020             


Trp Gly  Asp Tyr Leu Gln Tyr  Asp Lys Pro Tyr Tyr  Met Leu Asn 
    1025                 1030                 1035             


Leu Tyr  Asp Pro Asn Lys Tyr  Val Asp Val Asn Asn  Val Gly Ile 
    1040                 1045                 1050             


Arg Gly  Tyr Met Tyr Leu Lys  Gly Pro Arg Gly Ser  Val Met Thr 
    1055                 1060                 1065             


Thr Asn  Ile Tyr Leu Asn Ser  Ser Leu Tyr Arg Gly  Thr Lys Phe 
    1070                 1075                 1080             


Ile Ile  Lys Lys Tyr Ala Ser  Gly Asn Lys Asp Asn  Ile Val Arg 
    1085                 1090                 1095             


Asn Asn  Asp Arg Val Tyr Ile  Asn Val Val Val Lys  Asn Lys Glu 
    1100                 1105                 1110             


Tyr Arg  Leu Ala Thr Asn Ala  Ser Gln Ala Gly Val  Glu Lys Ile 
    1115                 1120                 1125             


Leu Ser  Ala Leu Glu Ile Pro  Asp Val Gly Asn Leu  Ser Gln Val 
    1130                 1135                 1140             


Val Val  Met Lys Ser Lys Asn  Asp Gln Gly Ile Thr  Asn Lys Cys 
    1145                 1150                 1155             


Lys Met  Asn Leu Gln Asp Asn  Asn Gly Asn Asp Ile  Gly Phe Ile 
    1160                 1165                 1170             


Gly Phe  His Gln Phe Asn Asn  Ile Ala Lys Leu Val  Ala Ser Asn 
    1175                 1180                 1185             


Trp Tyr  Asn Arg Gln Ile Glu  Arg Ser Ser Arg Thr  Leu Gly Cys 
    1190                 1195                 1200             


Ser Trp  Glu Phe Ile Pro Val  Asp Asp Gly Trp Gly  Glu Arg Pro 
    1205                 1210                 1215             


Leu Gly  Ala Gly Trp Ser His  Pro Gln Phe Glu Lys  
    1220                 1225                 1230 


<210>  7
<211>  1296
<212>  PRT
<213>  Clostridium Botulinum

<400>  7

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser 
                885                 890                 895     


Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn 
            900                 905                 910         


Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu 
        915                 920                 925             


Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser 
    930                 935                 940                 


Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn 
945                 950                 955                 960 


Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val 
                965                 970                 975     


Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu 
            980                 985                 990         


Ile Lys Gln Arg Val Val Phe Lys  Tyr Ser Gln Met Ile  Asn Ile Ser 
        995                 1000                 1005             


Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr Ile Thr  Asn Asn Arg 
    1010                 1015                 1020             


Leu Asn  Asn Ser Lys Ile Tyr  Ile Asn Gly Arg Leu  Ile Asp Gln 
    1025                 1030                 1035             


Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His Ala Ser  Asn Asn Ile 
    1040                 1045                 1050             


Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr His Arg  Tyr Ile Trp 
    1055                 1060                 1065             


Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu Leu Asn  Glu Lys Glu 
    1070                 1075                 1080             


Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn Ser Gly  Ile Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp Lys Pro  Tyr Tyr Met 
    1100                 1105                 1110             


Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val Asp Val  Asn Asn Val 
    1115                 1120                 1125             


Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly Pro Arg  Gly Ser Val 
    1130                 1135                 1140             


Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser Leu Tyr  Arg Gly Thr 
    1145                 1150                 1155             


Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly Asn Lys  Asp Asn Ile 
    1160                 1165                 1170             


Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn Val Val  Val Lys Asn 
    1175                 1180                 1185             


Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser Gln Ala  Gly Val Glu 
    1190                 1195                 1200             


Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Asp Val Gly  Asn Leu Ser 
    1205                 1210                 1215             


Gln Val  Val Val Met Lys Ser  Lys Asn Asp Gln Gly  Ile Thr Asn 
    1220                 1225                 1230             


Lys Cys  Lys Met Asn Leu Gln  Asp Asn Asn Gly Asn  Asp Ile Gly 
    1235                 1240                 1245             


Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile Ala Lys  Leu Val Ala 
    1250                 1255                 1260             


Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg Ser Ser  Arg Thr Leu 
    1265                 1270                 1275             


Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1280                 1285                 1290             


Arg Pro  Leu 
    1295     


<210>  8
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  Enterokinase cleavage site

<400>  8

Asp Asp Asp Asp Lys 
1               5   


<210>  9
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Spacer or linker sequence

<400>  9

Ala Arg Gly Gly Ala Ser Gly 
1               5           


<210>  10
<211>  8971
<212>  DNA
<213>  Artificial

<220>
<223>  BoNT/Aadtev vector pFB1SB3ATEV

<400>  10
gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc       60

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc      120

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt      180

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg      240

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt      300

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta      360

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt      420

aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat      480

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg      540

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa      600

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac      660

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac      720

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt      780

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc      840

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca      900

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc      960

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     1020

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     1080

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     1140

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     1200

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     1260

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     1320

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     1380

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     1440

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     1500

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     1560

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     1620

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     1680

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     1740

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     1800

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     1860

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     1920

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     1980

tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg     2040

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     2100

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     2160

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     2220

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     2280

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     2340

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg     2400

cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct     2460

ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga     2520

caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag     2580

acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt     2640

tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga     2700

ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac     2760

aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg     2820

tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg     2880

ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca     2940

tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact     3000

gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc     3060

gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta     3120

cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct     3180

ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg     3240

agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg     3300

ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca     3360

tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa     3420

acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa     3480

ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca     3540

ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac     3600

cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc     3660

ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg     3720

cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt     3780

ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt     3840

gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa     3900

tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt     3960

ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca     4020

ccatcgggcg cggatcccgg tccgttcgaa ccagaactct ggaagcttaa ctcctaaaaa     4080

accgccacca tgaaattctt agtcaacgtt gcccttgttt ttatggtcgt atacatttct     4140

tacatctatg cggccgctca tcaccaccat catcaccatc accaccacac gcgtgaaaac     4200

ctgtattttc agggcgccgg tgactccctg tcttggctgc tccgtctgct caacgcgcgc     4260

ggtggagcta gcggcccgtt cgttaacaaa caatttaact acaaggatcc tgtcaatggt     4320

gtggacattg cctatattaa gatcccgaat gcgggtcaga tgcaacccgt gaaagcattc     4380

aagatccaca acaaaatctg ggtcatccct gaacgtgaca ctttcacaaa ccctgaagag     4440

ggcgacctca accctccccc agaagccaaa caggttccgg tgtcttacta cgatagcacg     4500

tacttgtcca ccgataacga gaaggacaac tacctgaagg gagtgaccaa gttgtttgag     4560

aggatctact ctaccgatct cggacgtatg ctgctcacga gcattgtgcg cggtatccca     4620

ttctggggcg gttcaaccat tgatacagaa ctgaaagtca ttgacactaa ttgtatcaac     4680

gttattcaac cagatggcag ctaccgttcc gaggaattga acttggtcat cattggtcca     4740

tccgcagaca tcattcagtt tgaatgcaaa tccttcggtc acgaagtgct caacctgacg     4800

cgcaacggtt acggctccac ccagtacatc cgtttcagcc ctgatttcac atttggcttc     4860

gaggaaagcc tggaggttga caccaacccg ctcctgggtg ctggcaagtt tgcaaccgat     4920

cccgcggtga ctctcgctca tgctctgatc cacgccggac accgcctcta tggcatcgct     4980

atcaatccga accgcgtgtt caaagtgaat acgaacgcct actatgagat gagcggtctg     5040

gaggtttcct ttgaggaact gagaaccttc ggcggtcacg atgccaagtt catcgacagc     5100

ttgcaggaaa atgagtttcg cctgtactat tacaacaagt ttaaagacat cgcttccaca     5160

ttgaacaaag ccaagtcaat cgtgggtacg acagcttcat tgcagtatat gaagaatgtt     5220

ttcaaggaga aatacttgct gtcagaggat acctctggca agttctctgt ggacaaactg     5280

aaattcgaca aactgtacaa gatgctgacc gagatttata cggaagataa ctttgtgaaa     5340

ttcttcaaag tcctcaacag gaaaactgct ctgaactttg acaaggctgt gttcaagatc     5400

aacatcgtcc ccaaagttaa ctacacaatc tatgatggat tcaatctgag aaacaccaac     5460

ttggccgcca acttcaacgg ccaaaatacc gaaattaata acatgaattt caccaaactg     5520

aagaacttta ctggactgtt cgagttctac aagctgctct gcgtgcgtgg catcatcacc     5580

tcacatactc agtctctaga ccagggtggc gagaacctgt acttccaggg tgctctgaac     5640

gatctgtgta tcaaggtgaa taactgggat ctgttcttta gcccaagcga ggataacttc     5700

acgaacgatc tcaacaaagg tgaagagatc acgtctgata ccaatatcga agcggctgaa     5760

gagaatatct ccttggatct catccagcaa tattacctga cctttaactt cgataacgag     5820

cccgaaaaca tctccatcga gaacctcagc tcagacatca ttggtcagtt ggagctgatg     5880

ccaaacattg aacgcttccc caacggcaag aaatacgaac tcgacaagta tacgatgttt     5940

cattacttaa gagcgcagga gtttgaacac ggcaagagcc gcattgctct cactaactcc     6000

gtgaatgaag ccctgctcaa tccgtcaagg gtgtacacat tctttagctc cgactatgtc     6060

aagaaagtga acaaagccac cgaagcggca atgttcctgg gatgggttga acaactggtc     6120

tacgacttca ccgacgagac ctctgaggtg agcacaacgg acaagattgc tgacatcact     6180

atcattatcc cgtatattgg acctgccttg aatattggca acatgctcta caaagacgat     6240

ttcgttggtg ccctgatctt cagcggtgcc gtgatcctgt tggagttcat tcctgaaatc     6300

gccatccctg tgctgggcac gttcgctctg gtctcataca ttgcgaataa ggtcttgacc     6360

gtgcagacaa tcgataatgc cctctccaaa cgtaacgaaa aatgggacga ggtctacaaa     6420

tacatcgtga ccaactggct ggcaaaggtt aacacccaaa ttgatctgat ccgtaagaaa     6480

atgaaggagg ctttggagaa ccaggctgaa gctactaaag ccattatcaa ctaccagtat     6540

aatcagtata cagaagagga aaagaataac atcaatttca acatcgatga cttgtcctca     6600

aagctgaacg agtccatcaa caaagctatg atcaacatca acaaattcct gaatcagtgc     6660

tccgtgtctt acctgatgaa ctctatgatc ccatacggtg tgaagcgcct ggaggacttc     6720

gatgccagcc tgaaagacgc actgctcaaa tacatttacg ataatcgcgg cactttgatt     6780

ggccaagttg accgtctgaa ggacaaggtt aacaatacct tgtcaaccga tatccccttt     6840

caactgtcca aatacgttga taaccagcgc ttgctctcta ctttcaccga atacattaac     6900

aacattatca atacatcaat tctcaacctg cgctatgagt ccaatcatct gatcgatctg     6960

tctcgttacg ccagcaagat caacattggc agcaaagtga acttcgatcc gattgacaag     7020

aaccaaatcc agttgttcaa cctcgaaagc tccaaaatcg aagtgatcct gaagaatgcc     7080

atcgtctaca actccatgta tgaaaatttc tcaacttcat tctggattag aatcccgaaa     7140

tacttcaact caatctctct gaataacgaa tacacgatca ttaactgtat ggagaataac     7200

tctggttgga aggtttcctt gaactatgga gaaattatct ggactctgca agatacgcaa     7260

gagatcaaac agcgtgtggt ctttaaatac agccagatga ttaacatctc tgactacatc     7320

aacagatgga tctttgtcac cattacaaac aatcgcctga ataactccaa aatctacatc     7380

aacggtcgtc tgatcgacca gaaacctatt tcaaacctcg gcaacattca tgcttccaat     7440

aacatcatgt ttaagttgga tggttgccgc gatacccacc gttacatctg gatcaagtat     7500

ttcaatctgt tcgacaaaga actcaatgag aaagagatca aagacttgta tgataatcag     7560

tcaaactccg gcattctgaa agacttctgg ggcgattacc tccagtacga taagccatat     7620

tacatgctga atctctatga ccctaacaaa tatgtggacg tgaacaatgt cggtatccgt     7680

ggctacatgt acctcaaagg accacgtggt agcgttatga caaccaacat ctacctgaat     7740

agctccttgt atcgcggtac gaagttcatt atcaagaagt acgcttcagg caacaaggac     7800

aacatcgtga ggaacaatga tcgcgtgtac atcaacgtcg tggtgaagaa taaggaatac     7860

cgcttggcga ccaacgcttc tcaggctgga gttgagaaga tcctgagcgc cttggagatc     7920

ccagacgttg gcaacctgag ccaagtggtt gtgatgaaaa gcaagaatga ccagggaatc     7980

accaacaaat gcaaaatgaa cctgcaagac aacaacggca atgacatcgg tttcatcggt     8040

ttccaccagt ttaacaatat tgcgaagctg gtcgccagca actggtacaa caggcagatt     8100

gagaggtcat cccgtacctt aggatgctct tgggaattta tccccgtgga cgatggttgg     8160

ggcgagagac ccctgggcgc aggttggtcc caccctcagt tcgagaagta atagttaata     8220

gataataata gctcgaggca tgcgagctcc ctcaggaggc ctacgtcgac gagctcacta     8280

gtcgcggccg ctttcgaatc tagagcctgc agtctcgagg catgcggtac caagcttgtc     8340

gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta     8400

aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt     8460

aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca     8520

aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct     8580

tatcatgtct ggatctgatc actgcttgag cctaggagat ccgaaccaga taagtgaaat     8640

ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag     8700

ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc     8760

ccagttccca actattttgt ccgcccacag cggggcattt ttcttcctgt tatgttttta     8820

atcaaacatc ctgccaactc catgtgacaa accgtcatct tcggctactt tttctctgtc     8880

acagaatgaa aatttttctg tcatctcttc gttattaatg tttgtaattg actgaatatc     8940

aacgcttatt tgcagcctga atggcgaatg g                                    8971


<210>  11
<211>  7831
<212>  DNA
<213>  Artificial

<220>
<223>  Delta LC-Peptide-BoNT/Atev Vector pFB1SPepB2ATEV

<400>  11
gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc       60

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc      120

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt      180

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg      240

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt      300

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta      360

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt      420

aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat      480

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg      540

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa      600

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac      660

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac      720

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt      780

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc      840

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca      900

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc      960

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     1020

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     1080

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     1140

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     1200

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     1260

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     1320

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     1380

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     1440

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     1500

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     1560

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     1620

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     1680

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     1740

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     1800

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     1860

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     1920

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     1980

tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg     2040

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     2100

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     2160

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     2220

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     2280

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     2340

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg     2400

cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct     2460

ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga     2520

caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag     2580

acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt     2640

tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga     2700

ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac     2760

aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg     2820

tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg     2880

ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca     2940

tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact     3000

gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc     3060

gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta     3120

cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct     3180

ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg     3240

agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg     3300

ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca     3360

tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa     3420

acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa     3480

ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca     3540

ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac     3600

cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc     3660

ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg     3720

cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt     3780

ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt     3840

gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa     3900

tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt     3960

ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca     4020

ccatcgggcg cggatcccgg tccgttcgaa ccagaactct ggaagcttaa ctcctaaaaa     4080

accgccacca tgaaattctt agtcaacgtt gcccttgttt ttatggtcgt atacatttct     4140

tacatctatg cggccgctca tcaccaccat catcaccatc accaccacac gcgtgaaaac     4200

ctgtattttc agggcgccgg tgactccctg tcttggctgc tccgtctgct caacgcgcgc     4260

ggtggagcta gcggcggtac caatggcaac ggtaacggtg gtaatctgag aaacaccaac     4320

ttggccgcca acttcaacgg ccaaaatacc gaaattaata acatgaattt caccaaactg     4380

aagaacttta ctggactgtt cgagttctac aagctgctct gcgtgcgtgg catcatcacc     4440

tcacatactc agtctctaga ccagggtggc gagaacctgt acttccaggg tgctctgaac     4500

gatctgtgta tcaaggtgaa taactgggat ctgttcttta gcccaagcga ggataacttc     4560

acgaacgatc tcaacaaagg tgaagagatc acgtctgata ccaatatcga agcggctgaa     4620

gagaatatct ccttggatct catccagcaa tattacctga cctttaactt cgataacgag     4680

cccgaaaaca tctccatcga gaacctcagc tcagacatca ttggtcagtt ggagctgatg     4740

ccaaacattg aacgcttccc caacggcaag aaatacgaac tcgacaagta tacgatgttt     4800

cattacttaa gagcgcagga gtttgaacac ggcaagagcc gcattgctct cactaactcc     4860

gtgaatgaag ccctgctcaa tccgtcaagg gtgtacacat tctttagctc cgactatgtc     4920

aagaaagtga acaaagccac cgaagcggca atgttcctgg gatgggttga acaactggtc     4980

tacgacttca ccgacgagac ctctgaggtg agcacaacgg acaagattgc tgacatcact     5040

atcattatcc cgtatattgg acctgccttg aatattggca acatgctcta caaagacgat     5100

ttcgttggtg ccctgatctt cagcggtgcc gtgatcctgt tggagttcat tcctgaaatc     5160

gccatccctg tgctgggcac gttcgctctg gtctcataca ttgcgaataa ggtcttgacc     5220

gtgcagacaa tcgataatgc cctctccaaa cgtaacgaaa aatgggacga ggtctacaaa     5280

tacatcgtga ccaactggct ggcaaaggtt aacacccaaa ttgatctgat ccgtaagaaa     5340

atgaaggagg ctttggagaa ccaggctgaa gctactaaag ccattatcaa ctaccagtat     5400

aatcagtata cagaagagga aaagaataac atcaatttca acatcgatga cttgtcctca     5460

aagctgaacg agtccatcaa caaagctatg atcaacatca acaaattcct gaatcagtgc     5520

tccgtgtctt acctgatgaa ctctatgatc ccatacggtg tgaagcgcct ggaggacttc     5580

gatgccagcc tgaaagacgc actgctcaaa tacatttacg ataatcgcgg cactttgatt     5640

ggccaagttg accgtctgaa ggacaaggtt aacaatacct tgtcaaccga tatccccttt     5700

caactgtcca aatacgttga taaccagcgc ttgctctcta ctttcaccga atacattaac     5760

aacattatca atacatcaat tctcaacctg cgctatgagt ccaatcatct gatcgatctg     5820

tctcgttacg ccagcaagat caacattggc agcaaagtga acttcgatcc gattgacaag     5880

aaccaaatcc agttgttcaa cctcgaaagc tccaaaatcg aagtgatcct gaagaatgcc     5940

atcgtctaca actccatgta tgaaaatttc tcaacttcat tctggattag aatcccgaaa     6000

tacttcaact caatctctct gaataacgaa tacacgatca ttaactgtat ggagaataac     6060

tctggttgga aggtttcctt gaactatgga gaaattatct ggactctgca agatacgcaa     6120

gagatcaaac agcgtgtggt ctttaaatac agccagatga ttaacatctc tgactacatc     6180

aacagatgga tctttgtcac cattacaaac aatcgcctga ataactccaa aatctacatc     6240

aacggtcgtc tgatcgacca gaaacctatt tcaaacctcg gcaacattca tgcttccaat     6300

aacatcatgt ttaagttgga tggttgccgc gatacccacc gttacatctg gatcaagtat     6360

ttcaatctgt tcgacaaaga actcaatgag aaagagatca aagacttgta tgataatcag     6420

tcaaactccg gcattctgaa agacttctgg ggcgattacc tccagtacga taagccatat     6480

tacatgctga atctctatga ccctaacaaa tatgtggacg tgaacaatgt cggtatccgt     6540

ggctacatgt acctcaaagg accacgtggt agcgttatga caaccaacat ctacctgaat     6600

agctccttgt atcgcggtac gaagttcatt atcaagaagt acgcttcagg caacaaggac     6660

aacatcgtga ggaacaatga tcgcgtgtac atcaacgtcg tggtgaagaa taaggaatac     6720

cgcttggcga ccaacgcttc tcaggctgga gttgagaaga tcctgagcgc cttggagatc     6780

ccagacgttg gcaacctgag ccaagtggtt gtgatgaaaa gcaagaatga ccagggaatc     6840

accaacaaat gcaaaatgaa cctgcaagac aacaacggca atgacatcgg tttcatcggt     6900

ttccaccagt ttaacaatat tgcgaagctg gtcgccagca actggtacaa caggcagatt     6960

gagaggtcat cccgtacctt aggatgctct tgggaattta tccccgtgga cgatggttgg     7020

ggcgagagac ccctgggcgc aggttggtcc caccctcagt tcgagaagta atagttaata     7080

gataataata gctcgaggca tgcgagctcc ctcaggaggc ctacgtcgac gagctcacta     7140

gtcgcggccg ctttcgaatc tagagcctgc agtctcgagg catgcggtac caagcttgtc     7200

gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta     7260

aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt     7320

aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca     7380

aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct     7440

tatcatgtct ggatctgatc actgcttgag cctaggagat ccgaaccaga taagtgaaat     7500

ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag     7560

ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc     7620

ccagttccca actattttgt ccgcccacag cggggcattt ttcttcctgt tatgttttta     7680

atcaaacatc ctgccaactc catgtgacaa accgtcatct tcggctactt tttctctgtc     7740

acagaatgaa aatttttctg tcatctcttc gttattaatg tttgtaattg actgaatatc     7800

aacgcttatt tgcagcctga atggcgaatg g                                    7831


<210>  12
<211>  8542
<212>  DNA
<213>  Artificial

<220>
<223>  Delta LC-GFP-BoNT/Atev Vector pFB1SGFPB2ATEV

<400>  12
gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc       60

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc      120

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt      180

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg      240

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt      300

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta      360

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt      420

aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat      480

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg      540

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa      600

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac      660

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac      720

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt      780

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc      840

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca      900

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc      960

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     1020

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     1080

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     1140

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     1200

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     1260

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     1320

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     1380

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     1440

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     1500

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     1560

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     1620

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     1680

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     1740

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     1800

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     1860

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     1920

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     1980

tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg     2040

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     2100

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     2160

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     2220

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     2280

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     2340

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg     2400

cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct     2460

ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga     2520

caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag     2580

acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt     2640

tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga     2700

ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac     2760

aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg     2820

tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg     2880

ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca     2940

tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact     3000

gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc     3060

gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta     3120

cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct     3180

ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg     3240

agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg     3300

ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca     3360

tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa     3420

acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa     3480

ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca     3540

ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac     3600

cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc     3660

ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg     3720

cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt     3780

ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt     3840

gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa     3900

tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt     3960

ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca     4020

ccatcgggcg cggatcccgg tccgttcgaa ccagaactct ggaagcttaa ctcctaaaaa     4080

accgccacca tgaaattctt agtcaacgtt gcccttgttt ttatggtcgt atacatttct     4140

tacatctatg cggccgctca tcaccaccat catcaccatc accaccacac gcgtgaaaac     4200

ctgtattttc agggcgccgg tgactccctg tcttggctgc tccgtctgct caacgcgcgc     4260

ggtggagcta gcgtgagcaa gggcgccgag ctgttcaccg gcatcgtgcc catcctgatc     4320

gagctgaatg gcgatgtgaa tggccacaag ttcagcgtga gcggcgaggg cgagggcgat     4380

gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcctgtgccc     4440

tggcccaccc tggtgaccac cctgagctac ggcgtgcagt gcttctcacg ctaccccgat     4500

cacatgaagc agcacgactt cttcaagagc gccatgcctg agggctacat ccaggagcgc     4560

accatcttct tcgaggatga cggcaactac aagtcgcgcg ccgaggtgaa gttcgagggc     4620

gataccctgg tgaatcgcat cgagctgacc ggcaccgatt tcaaggagga tggcaacatc     4680

ctgggcaata agatggagta caactacaac gcccacaatg tgtacatcat gaccgacaag     4740

gccaagaatg gcatcaaggt gaacttcaag atccgccaca acatcgagga tggcagcgtg     4800

cagctggccg accactacca gcagaatacc cccatcggcg atggccctgt gctgctgccc     4860

gataaccact acctgtccac ccagagcgcc ctgtccaagg accccaacga gaagcgcgat     4920

cacatgatct acttcggctt cgtgaccgcc gccgccatca cccacggcat ggatgagctg     4980

tacaagggta ccaatggcaa cggtaacggt ggtaatctga gaaacaccaa cttggccgcc     5040

aacttcaacg gccaaaatac cgaaattaat aacatgaatt tcaccaaact gaagaacttt     5100

actggactgt tcgagttcta caagctgctc tgcgtgcgtg gcatcatcac ctcacatact     5160

cagtctctag accagggtgg cgagaacctg tacttccagg gtgctctgaa cgatctgtgt     5220

atcaaggtga ataactggga tctgttcttt agcccaagcg aggataactt cacgaacgat     5280

ctcaacaaag gtgaagagat cacgtctgat accaatatcg aagcggctga agagaatatc     5340

tccttggatc tcatccagca atattacctg acctttaact tcgataacga gcccgaaaac     5400

atctccatcg agaacctcag ctcagacatc attggtcagt tggagctgat gccaaacatt     5460

gaacgcttcc ccaacggcaa gaaatacgaa ctcgacaagt atacgatgtt tcattactta     5520

agagcgcagg agtttgaaca cggcaagagc cgcattgctc tcactaactc cgtgaatgaa     5580

gccctgctca atccgtcaag ggtgtacaca ttctttagct ccgactatgt caagaaagtg     5640

aacaaagcca ccgaagcggc aatgttcctg ggatgggttg aacaactggt ctacgacttc     5700

accgacgaga cctctgaggt gagcacaacg gacaagattg ctgacatcac tatcattatc     5760

ccgtatattg gacctgcctt gaatattggc aacatgctct acaaagacga tttcgttggt     5820

gccctgatct tcagcggtgc cgtgatcctg ttggagttca ttcctgaaat cgccatccct     5880

gtgctgggca cgttcgctct ggtctcatac attgcgaata aggtcttgac cgtgcagaca     5940

atcgataatg ccctctccaa acgtaacgaa aaatgggacg aggtctacaa atacatcgtg     6000

accaactggc tggcaaaggt taacacccaa attgatctga tccgtaagaa aatgaaggag     6060

gctttggaga accaggctga agctactaaa gccattatca actaccagta taatcagtat     6120

acagaagagg aaaagaataa catcaatttc aacatcgatg acttgtcctc aaagctgaac     6180

gagtccatca acaaagctat gatcaacatc aacaaattcc tgaatcagtg ctccgtgtct     6240

tacctgatga actctatgat cccatacggt gtgaagcgcc tggaggactt cgatgccagc     6300

ctgaaagacg cactgctcaa atacatttac gataatcgcg gcactttgat tggccaagtt     6360

gaccgtctga aggacaaggt taacaatacc ttgtcaaccg atatcccctt tcaactgtcc     6420

aaatacgttg ataaccagcg cttgctctct actttcaccg aatacattaa caacattatc     6480

aatacatcaa ttctcaacct gcgctatgag tccaatcatc tgatcgatct gtctcgttac     6540

gccagcaaga tcaacattgg cagcaaagtg aacttcgatc cgattgacaa gaaccaaatc     6600

cagttgttca acctcgaaag ctccaaaatc gaagtgatcc tgaagaatgc catcgtctac     6660

aactccatgt atgaaaattt ctcaacttca ttctggatta gaatcccgaa atacttcaac     6720

tcaatctctc tgaataacga atacacgatc attaactgta tggagaataa ctctggttgg     6780

aaggtttcct tgaactatgg agaaattatc tggactctgc aagatacgca agagatcaaa     6840

cagcgtgtgg tctttaaata cagccagatg attaacatct ctgactacat caacagatgg     6900

atctttgtca ccattacaaa caatcgcctg aataactcca aaatctacat caacggtcgt     6960

ctgatcgacc agaaacctat ttcaaacctc ggcaacattc atgcttccaa taacatcatg     7020

tttaagttgg atggttgccg cgatacccac cgttacatct ggatcaagta tttcaatctg     7080

ttcgacaaag aactcaatga gaaagagatc aaagacttgt atgataatca gtcaaactcc     7140

ggcattctga aagacttctg gggcgattac ctccagtacg ataagccata ttacatgctg     7200

aatctctatg accctaacaa atatgtggac gtgaacaatg tcggtatccg tggctacatg     7260

tacctcaaag gaccacgtgg tagcgttatg acaaccaaca tctacctgaa tagctccttg     7320

tatcgcggta cgaagttcat tatcaagaag tacgcttcag gcaacaagga caacatcgtg     7380

aggaacaatg atcgcgtgta catcaacgtc gtggtgaaga ataaggaata ccgcttggcg     7440

accaacgctt ctcaggctgg agttgagaag atcctgagcg ccttggagat cccagacgtt     7500

ggcaacctga gccaagtggt tgtgatgaaa agcaagaatg accagggaat caccaacaaa     7560

tgcaaaatga acctgcaaga caacaacggc aatgacatcg gtttcatcgg tttccaccag     7620

tttaacaata ttgcgaagct ggtcgccagc aactggtaca acaggcagat tgagaggtca     7680

tcccgtacct taggatgctc ttgggaattt atccccgtgg acgatggttg gggcgagaga     7740

cccctgggcg caggttggtc ccaccctcag ttcgagaagt aatagttaat agataataat     7800

agctcgaggc atgcgagctc cctcaggagg cctacgtcga cgagctcact agtcgcggcc     7860

gctttcgaat ctagagcctg cagtctcgag gcatgcggta ccaagcttgt cgagaagtac     7920

tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc     7980

ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt     8040

attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca     8100

tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc     8160

tggatctgat cactgcttga gcctaggaga tccgaaccag ataagtgaaa tctagttcca     8220

aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct     8280

attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc     8340

aactattttg tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat     8400

cctgccaact ccatgtgaca aaccgtcatc ttcggctact ttttctctgt cacagaatga     8460

aaatttttct gtcatctctt cgttattaat gtttgtaatt gactgaatat caacgcttat     8520

ttgcagcctg aatggcgaat gg                                              8542


<210>  13
<211>  2890
<212>  DNA
<213>  Artificial

<220>
<223>  Vector pLitmus28C1

<400>  13
gttaactacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt       60

tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca      120

ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt      180

ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga      240

tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa      300

gatccttgag agttttcgcc ccgaagaacg ttctccaatg atgagcactt ttaaagttct      360

gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat      420

acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga      480

tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc      540

caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat      600

gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa      660

cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac      720

tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa      780

agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc      840

tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc      900

ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag      960

acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta     1020

ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg     1080

aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta     1140

aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat     1200

aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca     1260

ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc     1320

ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta     1380

aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcg aacgtggcga     1440

gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca     1500

cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg     1560

atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg     1620

ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt     1680

ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg     1740

ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata     1800

ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca     1860

ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag     1920

tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc     1980

tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga     2040

tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg     2100

tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac     2160

gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg     2220

tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg     2280

ttcctggcct tttgctggcc ttttgctcac atgtaatgtg agttagctca ctcattaggc     2340

accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata     2400

acaatttcac acaggaaaca gctatgacca tgattacgcc aagctacgta atacgactca     2460

ctatagggca gatctgaaga cataagtcgg tccgttcgaa ccagaactct ggaagcttga     2520

cgcggccgct atccatggca cacgcgttca gctagcttag gcgcctatgc gcgctaaccg     2580

cggtcactta agtatgatat ctctctgcag ttacccgggc atgacgtcta tatgcatatt     2640

ctcgaggcat gcgagctccc tcaggaggcc tatagtgagt cgtattacgg actggccgtc     2700

gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca     2760

catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa     2820

cagttgcgca gcctgaatgg cgaatggcgc ttcgcttggt aataaagccc gcttcggcgg     2880

gctttttttt                                                            2890


<210>  14
<211>  6925
<212>  DNA
<213>  Artificial

<220>
<223>  Vector pLitSB3A

<400>  14
gttaactacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt       60

tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca      120

ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt      180

ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga      240

tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa      300

gatccttgag agttttcgcc ccgaagaacg ttctccaatg atgagcactt ttaaagttct      360

gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat      420

acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga      480

tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc      540

caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat      600

gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa      660

cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac      720

tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa      780

agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc      840

tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc      900

ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag      960

acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta     1020

ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg     1080

aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta     1140

aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat     1200

aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca     1260

ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc     1320

ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta     1380

aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcg aacgtggcga     1440

gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca     1500

cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg     1560

atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg     1620

ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt     1680

ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg     1740

ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata     1800

ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca     1860

ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag     1920

tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc     1980

tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga     2040

tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg     2100

tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac     2160

gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg     2220

tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg     2280

ttcctggcct tttgctggcc ttttgctcac atgtaatgtg agttagctca ctcattaggc     2340

accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata     2400

acaatttcac acaggaaaca gctatgacca tgattacgcc aagctacgta atacgactca     2460

ctatagggca gatctgaaga cataagtcgg tccgttcgaa ccagaactct ggaagcttaa     2520

ctcctaaaaa accgccacca tgaaattctt agtcaacgtt gcccttgttt ttatggtcgt     2580

atacatttct tacatctatg cggccgctca tcaccaccat catcaccatc accaccacac     2640

gcgtgaaaac ctgtattttc agggcgccgg tgactccctg tcttggctgc tccgtctgct     2700

caacgcgcgc ggtggagcta gcggcccgtt cgttaacaaa caatttaact acaaggatcc     2760

tgtcaatggt gtggacattg cctatattaa gatcccgaat gcgggtcaga tgcaacccgt     2820

gaaagcattc aagatccaca acaaaatctg ggtcatccct gaacgtgaca ctttcacaaa     2880

ccctgaagag ggcgacctca accctccccc agaagccaaa caggttccgg tgtcttacta     2940

cgatagcacg tacttgtcca ccgataacga gaaggacaac tacctgaagg gagtgaccaa     3000

gttgtttgag aggatctact ctaccgatct cggacgtatg ctgctcacga gcattgtgcg     3060

cggtatccca ttctggggcg gttcaaccat tgatacagaa ctgaaagtca ttgacactaa     3120

ttgtatcaac gttattcaac cagatggcag ctaccgttcc gaggaattga acttggtcat     3180

cattggtcca tccgcagaca tcattcagtt tgaatgcaaa tccttcggtc acgaagtgct     3240

caacctgacg cgcaacggtt acggctccac ccagtacatc cgtttcagcc ctgatttcac     3300

atttggcttc gaggaaagcc tggaggttga caccaacccg ctcctgggtg ctggcaagtt     3360

tgcaaccgat cccgcggtga ctctcgctca tgctctgatc cacgccggac accgcctcta     3420

tggcatcgct atcaatccga accgcgtgtt caaagtgaat acgaacgcct actatgagat     3480

gagcggtctg gaggtttcct ttgaggaact gagaaccttc ggcggtcacg atgccaagtt     3540

catcgacagc ttgcaggaaa atgagtttcg cctgtactat tacaacaagt ttaaagacat     3600

cgcttccaca ttgaacaaag ccaagtcaat cgtgggtacg acagcttcat tgcagtatat     3660

gaagaatgtt ttcaaggaga aatacttgct gtcagaggat acctctggca agttctctgt     3720

ggacaaactg aaattcgaca aactgtacaa gatgctgacc gagatttata cggaagataa     3780

ctttgtgaaa ttcttcaaag tcctcaacag gaaaactgct ctgaactttg acaaggctgt     3840

gttcaagatc aacatcgtcc ccaaagttaa ctacacaatc tatgatggat tcaatctgag     3900

aaacaccaac ttggccgcca acttcaacgg ccaaaatacc gaaattaata acatgaattt     3960

caccaaactg aagaacttta ctggactgtt cgagttctac aagctgctct gcgtgcgtgg     4020

catcatcacc tcacatactc agtctctaga ccagggttat aacgacgatg acgataaagc     4080

tctgaacgat ctgtgtatca aggtgaataa ctgggatctg ttctttagcc caagcgagga     4140

taacttcacg aacgatctca acaaaggtga agagatcacg tctgatacca atatcgaagc     4200

ggctgaagag aatatctcct tggatctcat ccagcaatat tacctgacct ttaacttcga     4260

taacgagccc gaaaacatct ccatcgagaa cctcagctca gacatcattg gtcagttgga     4320

gctgatgcca aacattgaac gcttccccaa cggcaagaaa tacgaactcg acaagtatac     4380

gatgtttcat tacttaagag cgcaggagtt tgaacacggc aagagccgca ttgctctcac     4440

taactccgtg aatgaagccc tgctcaatcc gtcaagggtg tacacattct ttagctccga     4500

ctatgtcaag aaagtgaaca aagccaccga agcggcaatg ttcctgggat gggttgaaca     4560

actggtctac gacttcaccg acgagacctc tgaggtgagc acaacggaca agattgctga     4620

catcactatc attatcccgt atattggacc tgccttgaat attggcaaca tgctctacaa     4680

agacgatttc gttggtgccc tgatcttcag cggtgccgtg atcctgttgg agttcattcc     4740

tgaaatcgcc atccctgtgc tgggcacgtt cgctctggtc tcatacattg cgaataaggt     4800

cttgaccgtg cagacaatcg ataatgccct ctccaaacgt aacgaaaaat gggacgaggt     4860

ctacaaatac atcgtgacca actggctggc aaaggttaac acccaaattg atctgatccg     4920

taagaaaatg aaggaggctt tggagaacca ggctgaagct actaaagcca ttatcaacta     4980

ccagtataat cagtatacag aagaggaaaa gaataacatc aatttcaaca tcgatgactt     5040

gtcctcaaag ctgaacgagt ccatcaacaa agctatgatc aacatcaaca aattcctgaa     5100

tcagtgctcc gtgtcttacc tgatgaactc tatgatccca tacggtgtga agcgcctgga     5160

ggacttcgat gccagcctga aagacgcact gctcaaatac atttacgata atcgcggcac     5220

tttgattggc caagttgacc gtctgaagga caaggttaac aataccttgt caaccgatat     5280

cccctttcaa ctgtccaaat acgttgataa ccagcgcttg ctctctactt tcaccgaata     5340

cattaacaac attatcaata catcaattct caacctgcgc tatgagtcca atcatctgat     5400

cgatctgtct cgttacgcca gcaagatcaa cattggcagc aaagtgaact tcgatccgat     5460

tgacaagaac caaatccagt tgttcaacct cgaaagctcc aaaatcgaag tgatcctgaa     5520

gaatgccatc gtctacaact ccatgtatga aaatttctca acttcattct ggattagaat     5580

cccgaaatac ttcaactcaa tctctctgaa taacgaatac acgatcatta actgtatgga     5640

gaataactct ggttggaagg tttccttgaa ctatggagaa attatctgga ctctgcaaga     5700

tacgcaagag atcaaacagc gtgtggtctt taaatacagc cagatgatta acatctctga     5760

ctacatcaac agatggatct ttgtcaccat tacaaacaat cgcctgaata actccaaaat     5820

ctacatcaac ggtcgtctga tcgaccagaa acctatttca aacctcggca acattcatgc     5880

ttccaataac atcatgttta agttggatgg ttgccgcgat acccaccgtt acatctggat     5940

caagtatttc aatctgttcg acaaagaact caatgagaaa gagatcaaag acttgtatga     6000

taatcagtca aactccggca ttctgaaaga cttctggggc gattacctcc agtacgataa     6060

gccatattac atgctgaatc tctatgaccc taacaaatat gtggacgtga acaatgtcgg     6120

tatccgtggc tacatgtacc tcaaaggacc acgtggtagc gttatgacaa ccaacatcta     6180

cctgaatagc tccttgtatc gcggtacgaa gttcattatc aagaagtacg cttcaggcaa     6240

caaggacaac atcgtgagga acaatgatcg cgtgtacatc aacgtcgtgg tgaagaataa     6300

ggaataccgc ttggcgacca acgcttctca ggctggagtt gagaagatcc tgagcgcctt     6360

ggagatccca gacgttggca acctgagcca agtggttgtg atgaaaagca agaatgacca     6420

gggaatcacc aacaaatgca aaatgaacct gcaagacaac aacggcaatg acatcggttt     6480

catcggtttc caccagttta acaatattgc gaagctggtc gccagcaact ggtacaacag     6540

gcagattgag aggtcatccc gtaccttagg atgctcttgg gaatttatcc ccgtggacga     6600

tggttggggc gagagacccc tgggcgcagg ttggtcccac cctcagttcg agaagtaata     6660

gttaatagat aataatagct cgaggcatgc gagctccctc aggaggatag tgagtcgtat     6720

tacggactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt     6780

aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc     6840

gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcttcgc ttggtaataa     6900

agcccgcttc ggcgggcttt ttttt                                           6925


<210>  15
<211>  8968
<212>  DNA
<213>  Artificial

<220>
<223>  BoNT/Aadek vector pFB1SB3AEK

<400>  15
gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc       60

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc      120

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt      180

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg      240

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt      300

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta      360

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt      420

aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat      480

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg      540

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa      600

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac      660

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac      720

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt      780

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc      840

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca      900

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc      960

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     1020

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     1080

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     1140

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     1200

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     1260

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     1320

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     1380

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     1440

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     1500

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     1560

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     1620

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     1680

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     1740

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     1800

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     1860

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     1920

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     1980

tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg     2040

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     2100

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     2160

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     2220

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     2280

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     2340

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg     2400

cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct     2460

ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga     2520

caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag     2580

acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt     2640

tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga     2700

ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac     2760

aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg     2820

tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg     2880

ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca     2940

tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact     3000

gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc     3060

gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta     3120

cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct     3180

ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg     3240

agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg     3300

ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca     3360

tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa     3420

acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa     3480

ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca     3540

ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac     3600

cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc     3660

ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg     3720

cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt     3780

ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt     3840

gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa     3900

tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt     3960

ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca     4020

ccatcgggcg cggatcccgg tccgttcgaa ccagaactct ggaagcttaa ctcctaaaaa     4080

accgccacca tgaaattctt agtcaacgtt gcccttgttt ttatggtcgt atacatttct     4140

tacatctatg cggccgctca tcaccaccat catcaccatc accaccacac gcgtgaaaac     4200

ctgtattttc agggcgccgg tgactccctg tcttggctgc tccgtctgct caacgcgcgc     4260

ggtggagcta gcggcccgtt cgttaacaaa caatttaact acaaggatcc tgtcaatggt     4320

gtggacattg cctatattaa gatcccgaat gcgggtcaga tgcaacccgt gaaagcattc     4380

aagatccaca acaaaatctg ggtcatccct gaacgtgaca ctttcacaaa ccctgaagag     4440

ggcgacctca accctccccc agaagccaaa caggttccgg tgtcttacta cgatagcacg     4500

tacttgtcca ccgataacga gaaggacaac tacctgaagg gagtgaccaa gttgtttgag     4560

aggatctact ctaccgatct cggacgtatg ctgctcacga gcattgtgcg cggtatccca     4620

ttctggggcg gttcaaccat tgatacagaa ctgaaagtca ttgacactaa ttgtatcaac     4680

gttattcaac cagatggcag ctaccgttcc gaggaattga acttggtcat cattggtcca     4740

tccgcagaca tcattcagtt tgaatgcaaa tccttcggtc acgaagtgct caacctgacg     4800

cgcaacggtt acggctccac ccagtacatc cgtttcagcc ctgatttcac atttggcttc     4860

gaggaaagcc tggaggttga caccaacccg ctcctgggtg ctggcaagtt tgcaaccgat     4920

cccgcggtga ctctcgctca tgctctgatc cacgccggac accgcctcta tggcatcgct     4980

atcaatccga accgcgtgtt caaagtgaat acgaacgcct actatgagat gagcggtctg     5040

gaggtttcct ttgaggaact gagaaccttc ggcggtcacg atgccaagtt catcgacagc     5100

ttgcaggaaa atgagtttcg cctgtactat tacaacaagt ttaaagacat cgcttccaca     5160

ttgaacaaag ccaagtcaat cgtgggtacg acagcttcat tgcagtatat gaagaatgtt     5220

ttcaaggaga aatacttgct gtcagaggat acctctggca agttctctgt ggacaaactg     5280

aaattcgaca aactgtacaa gatgctgacc gagatttata cggaagataa ctttgtgaaa     5340

ttcttcaaag tcctcaacag gaaaactgct ctgaactttg acaaggctgt gttcaagatc     5400

aacatcgtcc ccaaagttaa ctacacaatc tatgatggat tcaatctgag aaacaccaac     5460

ttggccgcca acttcaacgg ccaaaatacc gaaattaata acatgaattt caccaaactg     5520

aagaacttta ctggactgtt cgagttctac aagctgctct gcgtgcgtgg catcatcacc     5580

tcacatactc agtctctaga ccagggttat aacgacgatg acgataaagc tctgaacgat     5640

ctgtgtatca aggtgaataa ctgggatctg ttctttagcc caagcgagga taacttcacg     5700

aacgatctca acaaaggtga agagatcacg tctgatacca atatcgaagc ggctgaagag     5760

aatatctcct tggatctcat ccagcaatat tacctgacct ttaacttcga taacgagccc     5820

gaaaacatct ccatcgagaa cctcagctca gacatcattg gtcagttgga gctgatgcca     5880

aacattgaac gcttccccaa cggcaagaaa tacgaactcg acaagtatac gatgtttcat     5940

tacttaagag cgcaggagtt tgaacacggc aagagccgca ttgctctcac taactccgtg     6000

aatgaagccc tgctcaatcc gtcaagggtg tacacattct ttagctccga ctatgtcaag     6060

aaagtgaaca aagccaccga agcggcaatg ttcctgggat gggttgaaca actggtctac     6120

gacttcaccg acgagacctc tgaggtgagc acaacggaca agattgctga catcactatc     6180

attatcccgt atattggacc tgccttgaat attggcaaca tgctctacaa agacgatttc     6240

gttggtgccc tgatcttcag cggtgccgtg atcctgttgg agttcattcc tgaaatcgcc     6300

atccctgtgc tgggcacgtt cgctctggtc tcatacattg cgaataaggt cttgaccgtg     6360

cagacaatcg ataatgccct ctccaaacgt aacgaaaaat gggacgaggt ctacaaatac     6420

atcgtgacca actggctggc aaaggttaac acccaaattg atctgatccg taagaaaatg     6480

aaggaggctt tggagaacca ggctgaagct actaaagcca ttatcaacta ccagtataat     6540

cagtatacag aagaggaaaa gaataacatc aatttcaaca tcgatgactt gtcctcaaag     6600

ctgaacgagt ccatcaacaa agctatgatc aacatcaaca aattcctgaa tcagtgctcc     6660

gtgtcttacc tgatgaactc tatgatccca tacggtgtga agcgcctgga ggacttcgat     6720

gccagcctga aagacgcact gctcaaatac atttacgata atcgcggcac tttgattggc     6780

caagttgacc gtctgaagga caaggttaac aataccttgt caaccgatat cccctttcaa     6840

ctgtccaaat acgttgataa ccagcgcttg ctctctactt tcaccgaata cattaacaac     6900

attatcaata catcaattct caacctgcgc tatgagtcca atcatctgat cgatctgtct     6960

cgttacgcca gcaagatcaa cattggcagc aaagtgaact tcgatccgat tgacaagaac     7020

caaatccagt tgttcaacct cgaaagctcc aaaatcgaag tgatcctgaa gaatgccatc     7080

gtctacaact ccatgtatga aaatttctca acttcattct ggattagaat cccgaaatac     7140

ttcaactcaa tctctctgaa taacgaatac acgatcatta actgtatgga gaataactct     7200

ggttggaagg tttccttgaa ctatggagaa attatctgga ctctgcaaga tacgcaagag     7260

atcaaacagc gtgtggtctt taaatacagc cagatgatta acatctctga ctacatcaac     7320

agatggatct ttgtcaccat tacaaacaat cgcctgaata actccaaaat ctacatcaac     7380

ggtcgtctga tcgaccagaa acctatttca aacctcggca acattcatgc ttccaataac     7440

atcatgttta agttggatgg ttgccgcgat acccaccgtt acatctggat caagtatttc     7500

aatctgttcg acaaagaact caatgagaaa gagatcaaag acttgtatga taatcagtca     7560

aactccggca ttctgaaaga cttctggggc gattacctcc agtacgataa gccatattac     7620

atgctgaatc tctatgaccc taacaaatat gtggacgtga acaatgtcgg tatccgtggc     7680

tacatgtacc tcaaaggacc acgtggtagc gttatgacaa ccaacatcta cctgaatagc     7740

tccttgtatc gcggtacgaa gttcattatc aagaagtacg cttcaggcaa caaggacaac     7800

atcgtgagga acaatgatcg cgtgtacatc aacgtcgtgg tgaagaataa ggaataccgc     7860

ttggcgacca acgcttctca ggctggagtt gagaagatcc tgagcgcctt ggagatccca     7920

gacgttggca acctgagcca agtggttgtg atgaaaagca agaatgacca gggaatcacc     7980

aacaaatgca aaatgaacct gcaagacaac aacggcaatg acatcggttt catcggtttc     8040

caccagttta acaatattgc gaagctggtc gccagcaact ggtacaacag gcagattgag     8100

aggtcatccc gtaccttagg atgctcttgg gaatttatcc ccgtggacga tggttggggc     8160

gagagacccc tgggcgcagg ttggtcccac cctcagttcg agaagtaata gttaatagat     8220

aataatagct cgaggcatgc gagctccctc aggaggccta cgtcgacgag ctcactagtc     8280

gcggccgctt tcgaatctag agcctgcagt ctcgaggcat gcggtaccaa gcttgtcgag     8340

aagtactaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa     8400

aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac     8460

ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat     8520

aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat     8580

catgtctgga tctgatcact gcttgagcct aggagatccg aaccagataa gtgaaatcta     8640

gttccaaact attttgtcat ttttaatttt cgtattagct tacgacgcta cacccagttc     8700

ccatctattt tgtcactctt ccctaaataa tccttaaaaa ctccatttcc acccctccca     8760

gttcccaact attttgtccg cccacagcgg ggcatttttc ttcctgttat gtttttaatc     8820

aaacatcctg ccaactccat gtgacaaacc gtcatcttcg gctacttttt ctctgtcaca     8880

gaatgaaaat ttttctgtca tctcttcgtt attaatgttt gtaattgact gaatatcaac     8940

gcttatttgc agcctgaatg gcgaatgg                                        8968


<210>  16
<211>  103
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide C1-1S

<400>  16
gatctgaaga cataagtcgg tccgttcgaa ccagaactct ggaagcttga cgcggccgct       60

atccatggca cacgcgttca gctagcttag gcgcctatga cgt                        103


<210>  17
<211>  95
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide C1-1A

<400>  17
cataggcgcc taagctagct gaacgcgtgt gccatggata gcggccgcgt caagcttcca       60

gagttctggt tcgaacggac cgacttatgt cttca                                  95


<210>  18
<211>  108
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide C1-2S

<400>  18
gcgcctatgc gcgctaaccg cggtcactta agtatgatat ctctctgcag ttacccgggc       60

atgacgtcta tatgcatatt ctcgaggcat gcgagctccc tcaggagg                   108


<210>  19
<211>  104
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide C1-2A

<400>  19
cctcctgagg gagctcgcat gcctcgagaa tatgcatata gacgtcatgc ccgggtaact       60

gcagagagat atcatactta agtgaccgcg gttagcgcgc atag                       104


<210>  20
<211>  88
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide CP1S

<400>  20
agcttaactc ctaaaaaacc gccaccatga aattcttagt caacgttgcc cttgttttta       60

tggtcgtata catttcttac atctatgc                                          88


<210>  21
<211>  88
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide CP1A

<400>  21
ggccgcatag atgtaagaaa tgtatacgac cataaaaaca agggcaacgt tgactaagaa       60

tttcatggtg gcggtttttt aggagtta                                          88


<210>  22
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide CP2S

<400>  22
ggccgctcat caccaccatc atcaccatca ccaccaca                               38


<210>  23
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide CP2A

<400>  23
cgcgtgtggt ggtgatggtg atgatggtgg tgatgagc                               38


<210>  24
<211>  78
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide CP3S

<400>  24
cgcgtgaaaa cctgtatttt cagggcgccg gtgactccct gtcttggctg ctccgtctgc       60

tcaacgcgcg cggtggcg                                                     78


<210>  25
<211>  78
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide CP3A

<400>  25
ctagcgccac cgcgcgcgtt gagcagacgg agcagccaag acagggagtc accggcgccc       60

tgaaaataca ggttttca                                                     78


<210>  26
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide CP4S

<400>  26
agcttaccat gggtcatcac caccatcatc accatcacca ccaca                       45


<210>  27
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide CP4A

<400>  27
cgcgtgtggt ggtgatggtg atgatggtgg tgatgaccca tggta                       45


<210>  28
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide CP5S

<400>  28
agcttacgct gctccatcac caccatcatc accatcacca ccaca                       45


<210>  29
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide CP5A

<400>  29
cgcgtgtggt ggtgatggtg atgatggtgg tgatggagca tgcta                       45


<210>  30
<211>  10
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide CP6

<400>  30
taccctaggg                                                              10


<210>  31
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide CP7S

<400>  31
tcatgctagc gtgagcaagg gcgccgagct g                                      31


<210>  32
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide CP7A

<400>  32
tataggtacc cttgtacagc tcatccatgc cg                                     32


<210>  33
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Enhancer sequence

<400>  33
aactcctaaa aaaccgccac c                                                 21


<210>  34
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  Strep Tag II Sequence

<400>  34

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  35
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  cis-DNA sequence

<400>  35
aactcctaaa aaaccgccac c                                                 21


<210>  36
<211>  22
<212>  PRT
<213>  Artificial

<220>
<223>  Honeybee mellitin signal peptide

<400>  36

Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      


Ser Tyr Ile Tyr Ala Ala 
            20          


<210>  37
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  BoNT/Aadek Light Chain N-Terminus

<400>  37

Gly Ala Gly Asp Ser Leu 
1               5       


<210>  38
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  BoNT/Aadek Heavy Chain N-Terminus

<400>  38

Ala Leu Asn Asp Leu Cys 
1               5       


<210>  39
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  BoNT/Aadek Light Chain Tryptic Peptide Sequence

<400>  39

Leu Leu Cys Val Arg 
1               5   


<210>  40
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  BoNT/Aadek Heavy Chain Tryptic Peptide Sequence

<400>  40

Ala Leu Asn Asp Leu Cys Ile Lys 
1               5               


<210>  41
<211>  4
<212>  PRT
<213>  Artificial

<220>
<223>  Cleavage Product of BoNT/A Propeptide

<400>  41

Gly Tyr Asn Lys 
1               


<210>  42
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Excision Product of BoNT/A Precursor

<400>  42

Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
1               5                   10  


<210>  43
<211>  13
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide Insert With Substrate Sequence for BACE1

<400>  43

Ser Glu Ile Ser Tyr Glu Val Glu Phe Arg Trp Lys Lys 
1               5                   10              


<210>  44
<211>  4
<212>  PRT
<213>  Artificial

<220>
<223>  Insert Between N447 and K448 of BoNT/Aadek Construct

<400>  44

Asp Asp Asp Asp 
1               


<210>  45
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  Cargo Attachment Peptide

<400>  45

Gly Asp Ser Leu Asp Met Leu Glu Trp Ser Leu Met 
1               5                   10          


<210>  46
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  Cargo Attachment Peptide

<400>  46

Asp Ser Leu Asp Met Leu Glu Trp 
1               5               


<210>  47
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  Cargo Attachment Peptide

<400>  47

Met Ser Gly Leu Val Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His 
1               5                   10                  15      


<210>  48
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Cargo Attachment Peptide

<400>  48

Pro Lys Pro Gln Gln Phe Met 
1               5           


<210>  49
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  Cargo Attachment Peptide

<400>  49

Gly Gly Gly Gly Gly 
1               5   


