
                                SEQUENCE LISTING

<110> Ghanshani, Sanjiv
      Le, Linh Q.
      Liu, Li
      Steward, Lance E.

<120> Methods of Intracellular Conversion of
  Single-Chain Proteins into their Di-chain Form
  
<130> 18469 (BOT)

<150> US 61/286,963      
<151> 2010-01-25

<160> 107

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 1296
<212> PRT
<213> Clostridium botulinum Serotype A

<400> 1
Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly
 1               5                  10                  15      
Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro
            20                  25                  30          
Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg
        35                  40                  45              
Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu
    50                  55                  60                  
Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr
65                  70                  75                  80  
Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu
                85                  90                  95      
Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val
            100                 105                 110         
Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys
        115                 120                 125             
Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr
    130                 135                 140                 
Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile
145                 150                 155                 160 
Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr
                165                 170                 175     
Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe
            180                 185                 190         
Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu
        195                 200                 205             
Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu
    210                 215                 220                 
Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn
225                 230                 235                 240 
Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu
                245                 250                 255     
Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys
            260                 265                 270         
Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn
        275                 280                 285             
Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val
    290                 295                 300                 
Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys
305                 310                 315                 320 
Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu
                325                 330                 335     
Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp
            340                 345                 350         
Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn
        355                 360                 365             
Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr
    370                 375                 380                 
Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn
385                 390                 395                 400 
Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu
                405                 410                 415     
Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg
            420                 425                 430         
Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys
        435                 440                 445             
Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe
    450                 455                 460                 
Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu
465                 470                 475                 480 
Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu
                485                 490                 495     
Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro
            500                 505                 510         
Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu
        515                 520                 525             
Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu
    530                 535                 540                 
Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu
545                 550                 555                 560 
His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu
                565                 570                 575     
Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys
            580                 585                 590         
Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu
        595                 600                 605             
Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr
    610                 615                 620                 
Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala
625                 630                 635                 640 
Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu
                645                 650                 655     
Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala
            660                 665                 670         
Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys
        675                 680                 685             
Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu
    690                 695                 700                 
Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys
705                 710                 715                 720 
Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu
                725                 730                 735     
Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn
            740                 745                 750         
Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp
        755                 760                 765             
Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile
    770                 775                 780                 
Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met
785                 790                 795                 800 
Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys
                805                 810                 815     
Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly
            820                 825                 830         
Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp
        835                 840                 845             
Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser
    850                 855                 860                 
Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn
865                 870                 875                 880 
Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser
                885                 890                 895     
Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn
            900                 905                 910         
Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu
        915                 920                 925             
Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser
    930                 935                 940                 
Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn
945                 950                 955                 960 
Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val
                965                 970                 975     
Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu
            980                 985                 990         
Ile Lys Gln Arg Val Val Phe Lys Tyr Ser Gln Met Ile Asn Ile Ser
        995                 1000                1005            
Asp Tyr Ile Asn Arg Trp Ile Phe Val Thr Ile Thr Asn Asn Arg Leu
    1010                1015                1020                
Asn Asn Ser Lys Ile Tyr Ile Asn Gly Arg Leu Ile Asp Gln Lys Pro
1025                1030                1035                1040
Ile Ser Asn Leu Gly Asn Ile His Ala Ser Asn Asn Ile Met Phe Lys
                1045                1050                1055    
Leu Asp Gly Cys Arg Asp Thr His Arg Tyr Ile Trp Ile Lys Tyr Phe
            1060                1065                1070        
Asn Leu Phe Asp Lys Glu Leu Asn Glu Lys Glu Ile Lys Asp Leu Tyr
        1075                1080                1085            
Asp Asn Gln Ser Asn Ser Gly Ile Leu Lys Asp Phe Trp Gly Asp Tyr
    1090                1095                1100                
Leu Gln Tyr Asp Lys Pro Tyr Tyr Met Leu Asn Leu Tyr Asp Pro Asn
1105                1110                1115                1120
Lys Tyr Val Asp Val Asn Asn Val Gly Ile Arg Gly Tyr Met Tyr Leu
                1125                1130                1135    
Lys Gly Pro Arg Gly Ser Val Met Thr Thr Asn Ile Tyr Leu Asn Ser
            1140                1145                1150        
Ser Leu Tyr Arg Gly Thr Lys Phe Ile Ile Lys Lys Tyr Ala Ser Gly
        1155                1160                1165            
Asn Lys Asp Asn Ile Val Arg Asn Asn Asp Arg Val Tyr Ile Asn Val
    1170                1175                1180                
Val Val Lys Asn Lys Glu Tyr Arg Leu Ala Thr Asn Ala Ser Gln Ala
1185                1190                1195                1200
Gly Val Glu Lys Ile Leu Ser Ala Leu Glu Ile Pro Asp Val Gly Asn
                1205                1210                1215    
Leu Ser Gln Val Val Val Met Lys Ser Lys Asn Asp Gln Gly Ile Thr
            1220                1225                1230        
Asn Lys Cys Lys Met Asn Leu Gln Asp Asn Asn Gly Asn Asp Ile Gly
        1235                1240                1245            
Phe Ile Gly Phe His Gln Phe Asn Asn Ile Ala Lys Leu Val Ala Ser
    1250                1255                1260                
Asn Trp Tyr Asn Arg Gln Ile Glu Arg Ser Ser Arg Thr Leu Gly Cys
1265                1270                1275                1280
Ser Trp Glu Phe Ile Pro Val Asp Asp Gly Trp Gly Glu Arg Pro Leu
                1285                1290                1295    


<210> 2
<211> 1291
<212> PRT
<213> Clostridium botulinum Serotype B

<400> 2
Met Pro Val Thr Ile Asn Asn Phe Asn Tyr Asn Asp Pro Ile Asp Asn
 1               5                  10                  15      
Asn Asn Ile Ile Met Met Glu Pro Pro Phe Ala Arg Gly Thr Gly Arg
            20                  25                  30          
Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Ile Pro Glu
        35                  40                  45              
Arg Tyr Thr Phe Gly Tyr Lys Pro Glu Asp Phe Asn Lys Ser Ser Gly
    50                  55                  60                  
Ile Phe Asn Arg Asp Val Cys Glu Tyr Tyr Asp Pro Asp Tyr Leu Asn
65                  70                  75                  80  
Thr Asn Asp Lys Lys Asn Ile Phe Leu Gln Thr Met Ile Lys Leu Phe
                85                  90                  95      
Asn Arg Ile Lys Ser Lys Pro Leu Gly Glu Lys Leu Leu Glu Met Ile
            100                 105                 110         
Ile Asn Gly Ile Pro Tyr Leu Gly Asp Arg Arg Val Pro Leu Glu Glu
        115                 120                 125             
Phe Asn Thr Asn Ile Ala Ser Val Thr Val Asn Lys Leu Ile Ser Asn
    130                 135                 140                 
Pro Gly Glu Val Glu Arg Lys Lys Gly Ile Phe Ala Asn Leu Ile Ile
145                 150                 155                 160 
Phe Gly Pro Gly Pro Val Leu Asn Glu Asn Glu Thr Ile Asp Ile Gly
                165                 170                 175     
Ile Gln Asn His Phe Ala Ser Arg Glu Gly Phe Gly Gly Ile Met Gln
            180                 185                 190         
Met Lys Phe Cys Pro Glu Tyr Val Ser Val Phe Asn Asn Val Gln Glu
        195                 200                 205             
Asn Lys Gly Ala Ser Ile Phe Asn Arg Arg Gly Tyr Phe Ser Asp Pro
    210                 215                 220                 
Ala Leu Ile Leu Met His Glu Leu Ile His Val Leu His Gly Leu Tyr
225                 230                 235                 240 
Gly Ile Lys Val Asp Asp Leu Pro Ile Val Pro Asn Glu Lys Lys Phe
                245                 250                 255     
Phe Met Gln Ser Thr Asp Ala Ile Gln Ala Glu Glu Leu Tyr Thr Phe
            260                 265                 270         
Gly Gly Gln Asp Pro Ser Ile Ile Thr Pro Ser Thr Asp Lys Ser Ile
        275                 280                 285             
Tyr Asp Lys Val Leu Gln Asn Phe Arg Gly Ile Val Asp Arg Leu Asn
    290                 295                 300                 
Lys Val Leu Val Cys Ile Ser Asp Pro Asn Ile Asn Ile Asn Ile Tyr
305                 310                 315                 320 
Lys Asn Lys Phe Lys Asp Lys Tyr Lys Phe Val Glu Asp Ser Glu Gly
                325                 330                 335     
Lys Tyr Ser Ile Asp Val Glu Ser Phe Asp Lys Leu Tyr Lys Ser Leu
            340                 345                 350         
Met Phe Gly Phe Thr Glu Thr Asn Ile Ala Glu Asn Tyr Lys Ile Lys
        355                 360                 365             
Thr Arg Ala Ser Tyr Phe Ser Asp Ser Leu Pro Pro Val Lys Ile Lys
    370                 375                 380                 
Asn Leu Leu Asp Asn Glu Ile Tyr Thr Ile Glu Glu Gly Phe Asn Ile
385                 390                 395                 400 
Ser Asp Lys Asp Met Glu Lys Glu Tyr Arg Gly Gln Asn Lys Ala Ile
                405                 410                 415     
Asn Lys Gln Ala Tyr Glu Glu Ile Ser Lys Glu His Leu Ala Val Tyr
            420                 425                 430         
Lys Ile Gln Met Cys Lys Ser Val Lys Ala Pro Gly Ile Cys Ile Asp
        435                 440                 445             
Val Asp Asn Glu Asp Leu Phe Phe Ile Ala Asp Lys Asn Ser Phe Ser
    450                 455                 460                 
Asp Asp Leu Ser Lys Asn Glu Arg Ile Glu Tyr Asn Thr Gln Ser Asn
465                 470                 475                 480 
Tyr Ile Glu Asn Asp Phe Pro Ile Asn Glu Leu Ile Leu Asp Thr Asp
                485                 490                 495     
Leu Ile Ser Lys Ile Glu Leu Pro Ser Glu Asn Thr Glu Ser Leu Thr
            500                 505                 510         
Asp Phe Asn Val Asp Val Pro Val Tyr Glu Lys Gln Pro Ala Ile Lys
        515                 520                 525             
Lys Ile Phe Thr Asp Glu Asn Thr Ile Phe Gln Tyr Leu Tyr Ser Gln
    530                 535                 540                 
Thr Phe Pro Leu Asp Ile Arg Asp Ile Ser Leu Thr Ser Ser Phe Asp
545                 550                 555                 560 
Asp Ala Leu Leu Phe Ser Asn Lys Val Tyr Ser Phe Phe Ser Met Asp
                565                 570                 575     
Tyr Ile Lys Thr Ala Asn Lys Val Val Glu Ala Gly Leu Phe Ala Gly
            580                 585                 590         
Trp Val Lys Gln Ile Val Asn Asp Phe Val Ile Glu Ala Asn Lys Ser
        595                 600                 605             
Asn Thr Met Asp Lys Ile Ala Asp Ile Ser Leu Ile Val Pro Tyr Ile
    610                 615                 620                 
Gly Leu Ala Leu Asn Val Gly Asn Glu Thr Ala Lys Gly Asn Phe Glu
625                 630                 635                 640 
Asn Ala Phe Glu Ile Ala Gly Ala Ser Ile Leu Leu Glu Phe Ile Pro
                645                 650                 655     
Glu Leu Leu Ile Pro Val Val Gly Ala Phe Leu Leu Glu Ser Tyr Ile
            660                 665                 670         
Asp Asn Lys Asn Lys Ile Ile Lys Thr Ile Asp Asn Ala Leu Thr Lys
        675                 680                 685             
Arg Asn Glu Lys Trp Ser Asp Met Tyr Gly Leu Ile Val Ala Gln Trp
    690                 695                 700                 
Leu Ser Thr Val Asn Thr Gln Phe Tyr Thr Ile Lys Glu Gly Met Tyr
705                 710                 715                 720 
Lys Ala Leu Asn Tyr Gln Ala Gln Ala Leu Glu Glu Ile Ile Lys Tyr
                725                 730                 735     
Arg Tyr Asn Ile Tyr Ser Glu Lys Glu Lys Ser Asn Ile Asn Ile Asp
            740                 745                 750         
Phe Asn Asp Ile Asn Ser Lys Leu Asn Glu Gly Ile Asn Gln Ala Ile
        755                 760                 765             
Asp Asn Ile Asn Asn Phe Ile Asn Gly Cys Ser Val Ser Tyr Leu Met
    770                 775                 780                 
Lys Lys Met Ile Pro Leu Ala Val Glu Lys Leu Leu Asp Phe Asp Asn
785                 790                 795                 800 
Thr Leu Lys Lys Asn Leu Leu Asn Tyr Ile Asp Glu Asn Lys Leu Tyr
                805                 810                 815     
Leu Ile Gly Ser Ala Glu Tyr Glu Lys Ser Lys Val Asn Lys Tyr Leu
            820                 825                 830         
Lys Thr Ile Met Pro Phe Asp Leu Ser Ile Tyr Thr Asn Asp Thr Ile
        835                 840                 845             
Leu Ile Glu Met Phe Asn Lys Tyr Asn Ser Glu Ile Leu Asn Asn Ile
    850                 855                 860                 
Ile Leu Asn Leu Arg Tyr Lys Asp Asn Asn Leu Ile Asp Leu Ser Gly
865                 870                 875                 880 
Tyr Gly Ala Lys Val Glu Val Tyr Asp Gly Val Glu Leu Asn Asp Lys
                885                 890                 895     
Asn Gln Phe Lys Leu Thr Ser Ser Ala Asn Ser Lys Ile Arg Val Thr
            900                 905                 910         
Gln Asn Gln Asn Ile Ile Phe Asn Ser Val Phe Leu Asp Phe Ser Val
        915                 920                 925             
Ser Phe Trp Ile Arg Ile Pro Lys Tyr Lys Asn Asp Gly Ile Gln Asn
    930                 935                 940                 
Tyr Ile His Asn Glu Tyr Thr Ile Ile Asn Cys Met Lys Asn Asn Ser
945                 950                 955                 960 
Gly Trp Lys Ile Ser Ile Arg Gly Asn Arg Ile Ile Trp Thr Leu Ile
                965                 970                 975     
Asp Ile Asn Gly Lys Thr Lys Ser Val Phe Phe Glu Tyr Asn Ile Arg
            980                 985                 990         
Glu Asp Ile Ser Glu Tyr Ile Asn Arg Trp Phe Phe Val Thr Ile Thr
        995                 1000                1005            
Asn Asn Leu Asn Asn Ala Lys Ile Tyr Ile Asn Gly Lys Leu Glu Ser
    1010                1015                1020                
Asn Thr Asp Ile Lys Asp Ile Arg Glu Val Ile Ala Asn Gly Glu Ile
1025                1030                1035                1040
Ile Phe Lys Leu Asp Gly Asp Ile Asp Arg Thr Gln Phe Ile Trp Met
                1045                1050                1055    
Lys Tyr Phe Ser Ile Phe Asn Thr Glu Leu Ser Gln Ser Asn Ile Glu
            1060                1065                1070        
Glu Arg Tyr Lys Ile Gln Ser Tyr Ser Glu Tyr Leu Lys Asp Phe Trp
        1075                1080                1085            
Gly Asn Pro Leu Met Tyr Asn Lys Glu Tyr Tyr Met Phe Asn Ala Gly
    1090                1095                1100                
Asn Lys Asn Ser Tyr Ile Lys Leu Lys Lys Asp Ser Pro Val Gly Glu
1105                1110                1115                1120
Ile Leu Thr Arg Ser Lys Tyr Asn Gln Asn Ser Lys Tyr Ile Asn Tyr
                1125                1130                1135    
Arg Asp Leu Tyr Ile Gly Glu Lys Phe Ile Ile Arg Arg Lys Ser Asn
            1140                1145                1150        
Ser Gln Ser Ile Asn Asp Asp Ile Val Arg Lys Glu Asp Tyr Ile Tyr
        1155                1160                1165            
Leu Asp Phe Phe Asn Leu Asn Gln Glu Trp Arg Val Tyr Thr Tyr Lys
    1170                1175                1180                
Tyr Phe Lys Lys Glu Glu Glu Lys Leu Phe Leu Ala Pro Ile Ser Asp
1185                1190                1195                1200
Ser Asp Glu Phe Tyr Asn Thr Ile Gln Ile Lys Glu Tyr Asp Glu Gln
                1205                1210                1215    
Pro Thr Tyr Ser Cys Gln Leu Leu Phe Lys Lys Asp Glu Glu Ser Thr
            1220                1225                1230        
Asp Glu Ile Gly Leu Ile Gly Ile His Arg Phe Tyr Glu Ser Gly Ile
        1235                1240                1245            
Val Phe Glu Glu Tyr Lys Asp Tyr Phe Cys Ile Ser Lys Trp Tyr Leu
    1250                1255                1260                
Lys Glu Val Lys Arg Lys Pro Tyr Asn Leu Lys Leu Gly Cys Asn Trp
1265                1270                1275                1280
Gln Phe Ile Pro Lys Asp Glu Gly Trp Thr Glu
                1285                1290    


<210> 3
<211> 1291
<212> PRT
<213> Clostridium botulinum Serotype C1

<400> 3
Met Pro Ile Thr Ile Asn Asn Phe Asn Tyr Ser Asp Pro Val Asp Asn
 1               5                  10                  15      
Lys Asn Ile Leu Tyr Leu Asp Thr His Leu Asn Thr Leu Ala Asn Glu
            20                  25                  30          
Pro Glu Lys Ala Phe Arg Ile Thr Gly Asn Ile Trp Val Ile Pro Asp
        35                  40                  45              
Arg Phe Ser Arg Asn Ser Asn Pro Asn Leu Asn Lys Pro Pro Arg Val
    50                  55                  60                  
Thr Ser Pro Lys Ser Gly Tyr Tyr Asp Pro Asn Tyr Leu Ser Thr Asp
65                  70                  75                  80  
Ser Asp Lys Asp Pro Phe Leu Lys Glu Ile Ile Lys Leu Phe Lys Arg
                85                  90                  95      
Ile Asn Ser Arg Glu Ile Gly Glu Glu Leu Ile Tyr Arg Leu Ser Thr
            100                 105                 110         
Asp Ile Pro Phe Pro Gly Asn Asn Asn Thr Pro Ile Asn Thr Phe Asp
        115                 120                 125             
Phe Asp Val Asp Phe Asn Ser Val Asp Val Lys Thr Arg Gln Gly Asn
    130                 135                 140                 
Asn Trp Val Lys Thr Gly Ser Ile Asn Pro Ser Val Ile Ile Thr Gly
145                 150                 155                 160 
Pro Arg Glu Asn Ile Ile Asp Pro Glu Thr Ser Thr Phe Lys Leu Thr
                165                 170                 175     
Asn Asn Thr Phe Ala Ala Gln Glu Gly Phe Gly Ala Leu Ser Ile Ile
            180                 185                 190         
Ser Ile Ser Pro Arg Phe Met Leu Thr Tyr Ser Asn Ala Thr Asn Asp
        195                 200                 205             
Val Gly Glu Gly Arg Phe Ser Lys Ser Glu Phe Cys Met Asp Pro Ile
    210                 215                 220                 
Leu Ile Leu Met His Glu Leu Asn His Ala Met His Asn Leu Tyr Gly
225                 230                 235                 240 
Ile Ala Ile Pro Asn Asp Gln Thr Ile Ser Ser Val Thr Ser Asn Ile
                245                 250                 255     
Phe Tyr Ser Gln Tyr Asn Val Lys Leu Glu Tyr Ala Glu Ile Tyr Ala
            260                 265                 270         
Phe Gly Gly Pro Thr Ile Asp Leu Ile Pro Lys Ser Ala Arg Lys Tyr
        275                 280                 285             
Phe Glu Glu Lys Ala Leu Asp Tyr Tyr Arg Ser Ile Ala Lys Arg Leu
    290                 295                 300                 
Asn Ser Ile Thr Thr Ala Asn Pro Ser Ser Phe Asn Lys Tyr Ile Gly
305                 310                 315                 320 
Glu Tyr Lys Gln Lys Leu Ile Arg Lys Tyr Arg Phe Val Val Glu Ser
                325                 330                 335     
Ser Gly Glu Val Thr Val Asn Arg Asn Lys Phe Val Glu Leu Tyr Asn
            340                 345                 350         
Glu Leu Thr Gln Ile Phe Thr Glu Phe Asn Tyr Ala Lys Ile Tyr Asn
        355                 360                 365             
Val Gln Asn Arg Lys Ile Tyr Leu Ser Asn Val Tyr Thr Pro Val Thr
    370                 375                 380                 
Ala Asn Ile Leu Asp Asp Asn Val Tyr Asp Ile Gln Asn Gly Phe Asn
385                 390                 395                 400 
Ile Pro Lys Ser Asn Leu Asn Val Leu Phe Met Gly Gln Asn Leu Ser
                405                 410                 415     
Arg Asn Pro Ala Leu Arg Lys Val Asn Pro Glu Asn Met Leu Tyr Leu
            420                 425                 430         
Phe Thr Lys Phe Cys His Lys Ala Ile Asp Gly Arg Ser Leu Tyr Asn
        435                 440                 445             
Lys Thr Leu Asp Cys Arg Glu Leu Leu Val Lys Asn Thr Asp Leu Pro
    450                 455                 460                 
Phe Ile Gly Asp Ile Ser Asp Val Lys Thr Asp Ile Phe Leu Arg Lys
465                 470                 475                 480 
Asp Ile Asn Glu Glu Thr Glu Val Ile Tyr Tyr Pro Asp Asn Val Ser
                485                 490                 495     
Val Asp Gln Val Ile Leu Ser Lys Asn Thr Ser Glu His Gly Gln Leu
            500                 505                 510         
Asp Leu Leu Tyr Pro Ser Ile Asp Ser Glu Ser Glu Ile Leu Pro Gly
        515                 520                 525             
Glu Asn Gln Val Phe Tyr Asp Asn Arg Thr Gln Asn Val Asp Tyr Leu
    530                 535                 540                 
Asn Ser Tyr Tyr Tyr Leu Glu Ser Gln Lys Leu Ser Asp Asn Val Glu
545                 550                 555                 560 
Asp Phe Thr Phe Thr Arg Ser Ile Glu Glu Ala Leu Asp Asn Ser Ala
                565                 570                 575     
Lys Val Tyr Thr Tyr Phe Pro Thr Leu Ala Asn Lys Val Asn Ala Gly
            580                 585                 590         
Val Gln Gly Gly Leu Phe Leu Met Trp Ala Asn Asp Val Val Glu Asp
        595                 600                 605             
Phe Thr Thr Asn Ile Leu Arg Lys Asp Thr Leu Asp Lys Ile Ser Asp
    610                 615                 620                 
Val Ser Ala Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile Ser Asn
625                 630                 635                 640 
Ser Val Arg Arg Gly Asn Phe Thr Glu Ala Phe Ala Val Thr Gly Val
                645                 650                 655     
Thr Ile Leu Leu Glu Ala Phe Pro Glu Phe Thr Ile Pro Ala Leu Gly
            660                 665                 670         
Ala Phe Val Ile Tyr Ser Lys Val Gln Glu Arg Asn Glu Ile Ile Lys
        675                 680                 685             
Thr Ile Asp Asn Cys Leu Glu Gln Arg Ile Lys Arg Trp Lys Asp Ser
    690                 695                 700                 
Tyr Glu Trp Met Met Gly Thr Trp Leu Ser Arg Ile Ile Thr Gln Phe
705                 710                 715                 720 
Asn Asn Ile Ser Tyr Gln Met Tyr Asp Ser Leu Asn Tyr Gln Ala Gly
                725                 730                 735     
Ala Ile Lys Ala Lys Ile Asp Leu Glu Tyr Lys Lys Tyr Ser Gly Ser
            740                 745                 750         
Asp Lys Glu Asn Ile Lys Ser Gln Val Glu Asn Leu Lys Asn Ser Leu
        755                 760                 765             
Asp Val Lys Ile Ser Glu Ala Met Asn Asn Ile Asn Lys Phe Ile Arg
    770                 775                 780                 
Glu Cys Ser Val Thr Tyr Leu Phe Lys Asn Met Leu Pro Lys Val Ile
785                 790                 795                 800 
Asp Glu Leu Asn Glu Phe Asp Arg Asn Thr Lys Ala Lys Leu Ile Asn
                805                 810                 815     
Leu Ile Asp Ser His Asn Ile Ile Leu Val Gly Glu Val Asp Lys Leu
            820                 825                 830         
Lys Ala Lys Val Asn Asn Ser Phe Gln Asn Thr Ile Pro Phe Asn Ile
        835                 840                 845             
Phe Ser Tyr Thr Asn Asn Ser Leu Leu Lys Asp Ile Ile Asn Glu Tyr
    850                 855                 860                 
Phe Asn Asn Ile Asn Asp Ser Lys Ile Leu Ser Leu Gln Asn Arg Lys
865                 870                 875                 880 
Asn Thr Leu Val Asp Thr Ser Gly Tyr Asn Ala Glu Val Ser Glu Glu
                885                 890                 895     
Gly Asp Val Gln Leu Asn Pro Ile Phe Pro Phe Asp Phe Lys Leu Gly
            900                 905                 910         
Ser Ser Gly Glu Asp Arg Gly Lys Val Ile Val Thr Gln Asn Glu Asn
        915                 920                 925             
Ile Val Tyr Asn Ser Met Tyr Glu Ser Phe Ser Ile Ser Phe Trp Ile
    930                 935                 940                 
Arg Ile Asn Lys Trp Val Ser Asn Leu Pro Gly Tyr Thr Ile Ile Asp
945                 950                 955                 960 
Ser Val Lys Asn Asn Ser Gly Trp Ser Ile Gly Ile Ile Ser Asn Phe
                965                 970                 975     
Leu Val Phe Thr Leu Lys Gln Asn Glu Asp Ser Glu Gln Ser Ile Asn
            980                 985                 990         
Phe Ser Tyr Asp Ile Ser Asn Asn Ala Pro Gly Tyr Asn Lys Trp Phe
        995                 1000                1005            
Phe Val Thr Val Thr Asn Asn Met Met Gly Asn Met Lys Ile Tyr Ile
    1010                1015                1020                
Asn Gly Lys Leu Ile Asp Thr Ile Lys Val Lys Glu Leu Thr Gly Ile
1025                1030                1035                1040
Asn Phe Ser Lys Thr Ile Thr Phe Glu Ile Asn Lys Ile Pro Asp Thr
                1045                1050                1055    
Gly Leu Ile Thr Ser Asp Ser Asp Asn Ile Asn Met Trp Ile Arg Asp
            1060                1065                1070        
Phe Tyr Ile Phe Ala Lys Glu Leu Asp Gly Lys Asp Ile Asn Ile Leu
        1075                1080                1085            
Phe Asn Ser Leu Gln Tyr Thr Asn Val Val Lys Asp Tyr Trp Gly Asn
    1090                1095                1100                
Asp Leu Arg Tyr Asn Lys Glu Tyr Tyr Met Val Asn Ile Asp Tyr Leu
1105                1110                1115                1120
Asn Arg Tyr Met Tyr Ala Asn Ser Arg Gln Ile Val Phe Asn Thr Arg
                1125                1130                1135    
Arg Asn Asn Asn Asp Phe Asn Glu Gly Tyr Lys Ile Ile Ile Lys Arg
            1140                1145                1150        
Ile Arg Gly Asn Thr Asn Asp Thr Arg Val Arg Gly Gly Asp Ile Leu
        1155                1160                1165            
Tyr Phe Asp Met Thr Ile Asn Asn Lys Ala Tyr Asn Leu Phe Met Lys
    1170                1175                1180                
Asn Glu Thr Met Tyr Ala Asp Asn His Ser Thr Glu Asp Ile Tyr Ala
1185                1190                1195                1200
Ile Gly Leu Arg Glu Gln Thr Lys Asp Ile Asn Asp Asn Ile Ile Phe
                1205                1210                1215    
Gln Ile Gln Pro Met Asn Asn Thr Tyr Tyr Tyr Ala Ser Gln Ile Phe
            1220                1225                1230        
Lys Ser Asn Phe Asn Gly Glu Asn Ile Ser Gly Ile Cys Ser Ile Gly
        1235                1240                1245            
Thr Tyr Arg Phe Arg Leu Gly Gly Asp Trp Tyr Arg His Asn Tyr Leu
    1250                1255                1260                
Val Pro Thr Val Lys Gln Gly Asn Tyr Ala Ser Leu Leu Glu Ser Thr
1265                1270                1275                1280
Ser Thr His Trp Gly Phe Val Pro Val Ser Glu
                1285                1290    


<210> 4
<211> 1276
<212> PRT
<213> Clostridium botulinum Serotype D

<400> 4
Met Thr Trp Pro Val Lys Asp Phe Asn Tyr Ser Asp Pro Val Asn Asp
 1               5                  10                  15      
Asn Asp Ile Leu Tyr Leu Arg Ile Pro Gln Asn Lys Leu Ile Thr Thr
            20                  25                  30          
Pro Val Lys Ala Phe Met Ile Thr Gln Asn Ile Trp Val Ile Pro Glu
        35                  40                  45              
Arg Phe Ser Ser Asp Thr Asn Pro Ser Leu Ser Lys Pro Pro Arg Pro
    50                  55                  60                  
Thr Ser Lys Tyr Gln Ser Tyr Tyr Asp Pro Ser Tyr Leu Ser Thr Asp
65                  70                  75                  80  
Glu Gln Lys Asp Thr Phe Leu Lys Gly Ile Ile Lys Leu Phe Lys Arg
                85                  90                  95      
Ile Asn Glu Arg Asp Ile Gly Lys Lys Leu Ile Asn Tyr Leu Val Val
            100                 105                 110         
Gly Ser Pro Phe Met Gly Asp Ser Ser Thr Pro Glu Asp Thr Phe Asp
        115                 120                 125             
Phe Thr Arg His Thr Thr Asn Ile Ala Val Glu Lys Phe Glu Asn Gly
    130                 135                 140                 
Ser Trp Lys Val Thr Asn Ile Ile Thr Pro Ser Val Leu Ile Phe Gly
145                 150                 155                 160 
Pro Leu Pro Asn Ile Leu Asp Tyr Thr Ala Ser Leu Thr Leu Gln Gly
                165                 170                 175     
Gln Gln Ser Asn Pro Ser Phe Glu Gly Phe Gly Thr Leu Ser Ile Leu
            180                 185                 190         
Lys Val Ala Pro Glu Phe Leu Leu Thr Phe Ser Asp Val Thr Ser Asn
        195                 200                 205             
Gln Ser Ser Ala Val Leu Gly Lys Ser Ile Phe Cys Met Asp Pro Val
    210                 215                 220                 
Ile Ala Leu Met His Glu Leu Thr His Ser Leu His Gln Leu Tyr Gly
225                 230                 235                 240 
Ile Asn Ile Pro Ser Asp Lys Arg Ile Arg Pro Gln Val Ser Glu Gly
                245                 250                 255     
Phe Phe Ser Gln Asp Gly Pro Asn Val Gln Phe Glu Glu Leu Tyr Thr
            260                 265                 270         
Phe Gly Gly Leu Asp Val Glu Ile Ile Pro Gln Ile Glu Arg Ser Gln
        275                 280                 285             
Leu Arg Glu Lys Ala Leu Gly His Tyr Lys Asp Ile Ala Lys Arg Leu
    290                 295                 300                 
Asn Asn Ile Asn Lys Thr Ile Pro Ser Ser Trp Ile Ser Asn Ile Asp
305                 310                 315                 320 
Lys Tyr Lys Lys Ile Phe Ser Glu Lys Tyr Asn Phe Asp Lys Asp Asn
                325                 330                 335     
Thr Gly Asn Phe Val Val Asn Ile Asp Lys Phe Asn Ser Leu Tyr Ser
            340                 345                 350         
Asp Leu Thr Asn Val Met Ser Glu Val Val Tyr Ser Ser Gln Tyr Asn
        355                 360                 365             
Val Lys Asn Arg Thr His Tyr Phe Ser Arg His Tyr Leu Pro Val Phe
    370                 375                 380                 
Ala Asn Ile Leu Asp Asp Asn Ile Tyr Thr Ile Arg Asp Gly Phe Asn
385                 390                 395                 400 
Leu Thr Asn Lys Gly Phe Asn Ile Glu Asn Ser Gly Gln Asn Ile Glu
                405                 410                 415     
Arg Asn Pro Ala Leu Gln Lys Leu Ser Ser Glu Ser Val Val Asp Leu
            420                 425                 430         
Phe Thr Lys Val Cys Leu Arg Leu Thr Lys Asn Ser Arg Asp Asp Ser
        435                 440                 445             
Thr Cys Ile Lys Val Lys Asn Asn Arg Leu Pro Tyr Val Ala Asp Lys
    450                 455                 460                 
Asp Ser Ile Ser Gln Glu Ile Phe Glu Asn Lys Ile Ile Thr Asp Glu
465                 470                 475                 480 
Thr Asn Val Gln Asn Tyr Ser Asp Lys Phe Ser Leu Asp Glu Ser Ile
                485                 490                 495     
Leu Asp Gly Gln Val Pro Ile Asn Pro Glu Ile Val Asp Pro Leu Leu
            500                 505                 510         
Pro Asn Val Asn Met Glu Pro Leu Asn Leu Pro Gly Glu Glu Ile Val
        515                 520                 525             
Phe Tyr Asp Asp Ile Thr Lys Tyr Val Asp Tyr Leu Asn Ser Tyr Tyr
    530                 535                 540                 
Tyr Leu Glu Ser Gln Lys Leu Ser Asn Asn Val Glu Asn Ile Thr Leu
545                 550                 555                 560 
Thr Thr Ser Val Glu Glu Ala Leu Gly Tyr Ser Asn Lys Ile Tyr Thr
                565                 570                 575     
Phe Leu Pro Ser Leu Ala Glu Lys Val Asn Lys Gly Val Gln Ala Gly
            580                 585                 590         
Leu Phe Leu Asn Trp Ala Asn Glu Val Val Glu Asp Phe Thr Thr Asn
        595                 600                 605             
Ile Met Lys Lys Asp Thr Leu Asp Lys Ile Ser Asp Val Ser Val Ile
    610                 615                 620                 
Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile Gly Asn Ser Ala Leu Arg
625                 630                 635                 640 
Gly Asn Phe Asn Gln Ala Phe Ala Thr Ala Gly Val Ala Phe Leu Leu
                645                 650                 655     
Glu Gly Phe Pro Glu Phe Thr Ile Pro Ala Leu Gly Val Phe Thr Phe
            660                 665                 670         
Tyr Ser Ser Ile Gln Glu Arg Glu Lys Ile Ile Lys Thr Ile Glu Asn
        675                 680                 685             
Cys Leu Glu Gln Arg Val Lys Arg Trp Lys Asp Ser Tyr Gln Trp Met
    690                 695                 700                 
Val Ser Asn Trp Leu Ser Arg Ile Thr Thr Gln Phe Asn His Ile Asn
705                 710                 715                 720 
Tyr Gln Met Tyr Asp Ser Leu Ser Tyr Gln Ala Asp Ala Ile Lys Ala
                725                 730                 735     
Lys Ile Asp Leu Glu Tyr Lys Lys Tyr Ser Gly Ser Asp Lys Glu Asn
            740                 745                 750         
Ile Lys Ser Gln Val Glu Asn Leu Lys Asn Ser Leu Asp Val Lys Ile
        755                 760                 765             
Ser Glu Ala Met Asn Asn Ile Asn Lys Phe Ile Arg Glu Cys Ser Val
    770                 775                 780                 
Thr Tyr Leu Phe Lys Asn Met Leu Pro Lys Val Ile Asp Glu Leu Asn
785                 790                 795                 800 
Lys Phe Asp Leu Arg Thr Lys Thr Glu Leu Ile Asn Leu Ile Asp Ser
                805                 810                 815     
His Asn Ile Ile Leu Val Gly Glu Val Asp Arg Leu Lys Ala Lys Val
            820                 825                 830         
Asn Glu Ser Phe Glu Asn Thr Met Pro Phe Asn Ile Phe Ser Tyr Thr
        835                 840                 845             
Asn Asn Ser Leu Leu Lys Asp Ile Ile Asn Glu Tyr Phe Asn Ser Ile
    850                 855                 860                 
Asn Asp Ser Lys Ile Leu Ser Leu Gln Asn Lys Lys Asn Ala Leu Val
865                 870                 875                 880 
Asp Thr Ser Gly Tyr Asn Ala Glu Val Arg Val Gly Asp Asn Val Gln
                885                 890                 895     
Leu Asn Thr Ile Tyr Thr Asn Asp Phe Lys Leu Ser Ser Ser Gly Asp
            900                 905                 910         
Lys Ile Ile Val Asn Leu Asn Asn Asn Ile Leu Tyr Ser Ala Ile Tyr
        915                 920                 925             
Glu Asn Ser Ser Val Ser Phe Trp Ile Lys Ile Ser Lys Asp Leu Thr
    930                 935                 940                 
Asn Ser His Asn Glu Tyr Thr Ile Ile Asn Ser Ile Glu Gln Asn Ser
945                 950                 955                 960 
Gly Trp Lys Leu Cys Ile Arg Asn Gly Asn Ile Glu Trp Ile Leu Gln
                965                 970                 975     
Asp Val Asn Arg Lys Tyr Lys Ser Leu Ile Phe Asp Tyr Ser Glu Ser
            980                 985                 990         
Leu Ser His Thr Gly Tyr Thr Asn Lys Trp Phe Phe Val Thr Ile Thr
        995                 1000                1005            
Asn Asn Ile Met Gly Tyr Met Lys Leu Tyr Ile Asn Gly Glu Leu Lys
    1010                1015                1020                
Gln Ser Gln Lys Ile Glu Asp Leu Asp Glu Val Lys Leu Asp Lys Thr
1025                1030                1035                1040
Ile Val Phe Gly Ile Asp Glu Asn Ile Asp Glu Asn Gln Met Leu Trp
                1045                1050                1055    
Ile Arg Asp Phe Asn Ile Phe Ser Lys Glu Leu Ser Asn Glu Asp Ile
            1060                1065                1070        
Asn Ile Val Tyr Glu Gly Gln Ile Leu Arg Asn Val Ile Lys Asp Tyr
        1075                1080                1085            
Trp Gly Asn Pro Leu Lys Phe Asp Thr Glu Tyr Tyr Ile Ile Asn Asp
    1090                1095                1100                
Asn Tyr Ile Asp Arg Tyr Ile Ala Pro Glu Ser Asn Val Leu Val Leu
1105                1110                1115                1120
Val Gln Tyr Pro Asp Arg Ser Lys Leu Tyr Thr Gly Asn Pro Ile Thr
                1125                1130                1135    
Ile Lys Ser Val Ser Asp Lys Asn Pro Tyr Ser Arg Ile Leu Asn Gly
            1140                1145                1150        
Asp Asn Ile Ile Leu His Met Leu Tyr Asn Ser Arg Lys Tyr Met Ile
        1155                1160                1165            
Ile Arg Asp Thr Asp Thr Ile Tyr Ala Thr Gln Gly Gly Glu Cys Ser
    1170                1175                1180                
Gln Asn Cys Val Tyr Ala Leu Lys Leu Gln Ser Asn Leu Gly Asn Tyr
1185                1190                1195                1200
Gly Ile Gly Ile Phe Ser Ile Lys Asn Ile Val Ser Lys Asn Lys Tyr
                1205                1210                1215    
Cys Ser Gln Ile Phe Ser Ser Phe Arg Glu Asn Thr Met Leu Leu Ala
            1220                1225                1230        
Asp Ile Tyr Lys Pro Trp Arg Phe Ser Phe Lys Asn Ala Tyr Thr Pro
        1235                1240                1245            
Val Ala Val Thr Asn Tyr Glu Thr Lys Leu Leu Ser Thr Ser Ser Phe
    1250                1255                1260                
Trp Lys Phe Ile Ser Arg Asp Pro Gly Trp Val Glu
1265                1270                1275    


<210> 5
<211> 1252
<212> PRT
<213> Clostridium botulinum Serotype E

<400> 5
Met Pro Lys Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp Arg
 1               5                  10                  15      
Thr Ile Leu Tyr Ile Lys Pro Gly Gly Cys Gln Glu Phe Tyr Lys Ser
            20                  25                  30          
Phe Asn Ile Met Lys Asn Ile Trp Ile Ile Pro Glu Arg Asn Val Ile
        35                  40                  45              
Gly Thr Thr Pro Gln Asp Phe His Pro Pro Thr Ser Leu Lys Asn Gly
    50                  55                  60                  
Asp Ser Ser Tyr Tyr Asp Pro Asn Tyr Leu Gln Ser Asp Glu Glu Lys
65                  70                  75                  80  
Asp Arg Phe Leu Lys Ile Val Thr Lys Ile Phe Asn Arg Ile Asn Asn
                85                  90                  95      
Asn Leu Ser Gly Gly Ile Leu Leu Glu Glu Leu Ser Lys Ala Asn Pro
            100                 105                 110         
Tyr Leu Gly Asn Asp Asn Thr Pro Asp Asn Gln Phe His Ile Gly Asp
        115                 120                 125             
Ala Ser Ala Val Glu Ile Lys Phe Ser Asn Gly Ser Gln Asp Ile Leu
    130                 135                 140                 
Leu Pro Asn Val Ile Ile Met Gly Ala Glu Pro Asp Leu Phe Glu Thr
145                 150                 155                 160 
Asn Ser Ser Asn Ile Ser Leu Arg Asn Asn Tyr Met Pro Ser Asn His
                165                 170                 175     
Gly Phe Gly Ser Ile Ala Ile Val Thr Phe Ser Pro Glu Tyr Ser Phe
            180                 185                 190         
Arg Phe Asn Asp Asn Ser Met Asn Glu Phe Ile Gln Asp Pro Ala Leu
        195                 200                 205             
Thr Leu Met His Glu Leu Ile His Ser Leu His Gly Leu Tyr Gly Ala
    210                 215                 220                 
Lys Gly Ile Thr Thr Lys Tyr Thr Ile Thr Gln Lys Gln Asn Pro Leu
225                 230                 235                 240 
Ile Thr Asn Ile Arg Gly Thr Asn Ile Glu Glu Phe Leu Thr Phe Gly
                245                 250                 255     
Gly Thr Asp Leu Asn Ile Ile Thr Ser Ala Gln Ser Asn Asp Ile Tyr
            260                 265                 270         
Thr Asn Leu Leu Ala Asp Tyr Lys Lys Ile Ala Ser Lys Leu Ser Lys
        275                 280                 285             
Val Gln Val Ser Asn Pro Leu Leu Asn Pro Tyr Lys Asp Val Phe Glu
    290                 295                 300                 
Ala Lys Tyr Gly Leu Asp Lys Asp Ala Ser Gly Ile Tyr Ser Val Asn
305                 310                 315                 320 
Ile Asn Lys Phe Asn Asp Ile Phe Lys Lys Leu Tyr Ser Phe Thr Glu
                325                 330                 335     
Phe Asp Leu Ala Thr Lys Phe Gln Val Lys Cys Arg Gln Thr Tyr Ile
            340                 345                 350         
Gly Gln Tyr Lys Tyr Phe Lys Leu Ser Asn Leu Leu Asn Asp Ser Ile
        355                 360                 365             
Tyr Asn Ile Ser Glu Gly Tyr Asn Ile Asn Asn Leu Lys Val Asn Phe
    370                 375                 380                 
Arg Gly Gln Asn Ala Asn Leu Asn Pro Arg Ile Ile Thr Pro Ile Thr
385                 390                 395                 400 
Gly Arg Gly Leu Val Lys Lys Ile Ile Arg Phe Cys Lys Asn Ile Val
                405                 410                 415     
Ser Val Lys Gly Ile Arg Lys Ser Ile Cys Ile Glu Ile Asn Asn Gly
            420                 425                 430         
Glu Leu Phe Phe Val Ala Ser Glu Asn Ser Tyr Asn Asp Asp Asn Ile
        435                 440                 445             
Asn Thr Pro Lys Glu Ile Asp Asp Thr Val Thr Ser Asn Asn Asn Tyr
    450                 455                 460                 
Glu Asn Asp Leu Asp Gln Val Ile Leu Asn Phe Asn Ser Glu Ser Ala
465                 470                 475                 480 
Pro Gly Leu Ser Asp Glu Lys Leu Asn Leu Thr Ile Gln Asn Asp Ala
                485                 490                 495     
Tyr Ile Pro Lys Tyr Asp Ser Asn Gly Thr Ser Asp Ile Glu Gln His
            500                 505                 510         
Asp Val Asn Glu Leu Asn Val Phe Phe Tyr Leu Asp Ala Gln Lys Val
        515                 520                 525             
Pro Glu Gly Glu Asn Asn Val Asn Leu Thr Ser Ser Ile Asp Thr Ala
    530                 535                 540                 
Leu Leu Glu Gln Pro Lys Ile Tyr Thr Phe Phe Ser Ser Glu Phe Ile
545                 550                 555                 560 
Asn Asn Val Asn Lys Pro Val Gln Ala Ala Leu Phe Val Ser Trp Ile
                565                 570                 575     
Gln Gln Val Leu Val Asp Phe Thr Thr Glu Ala Asn Gln Lys Ser Thr
            580                 585                 590         
Val Asp Lys Ile Ala Asp Ile Ser Ile Val Val Pro Tyr Ile Gly Leu
        595                 600                 605             
Ala Leu Asn Ile Gly Asn Glu Ala Gln Lys Gly Asn Phe Lys Asp Ala
    610                 615                 620                 
Leu Glu Leu Leu Gly Ala Gly Ile Leu Leu Glu Phe Glu Pro Glu Leu
625                 630                 635                 640 
Leu Ile Pro Thr Ile Leu Val Phe Thr Ile Lys Ser Phe Leu Gly Ser
                645                 650                 655     
Ser Asp Asn Lys Asn Lys Val Ile Lys Ala Ile Asn Asn Ala Leu Lys
            660                 665                 670         
Glu Arg Asp Glu Lys Trp Lys Glu Val Tyr Ser Phe Ile Val Ser Asn
        675                 680                 685             
Trp Met Thr Lys Ile Asn Thr Gln Phe Asn Lys Arg Lys Glu Gln Met
    690                 695                 700                 
Tyr Gln Ala Leu Gln Asn Gln Val Asn Ala Ile Lys Thr Ile Ile Glu
705                 710                 715                 720 
Ser Lys Tyr Asn Ser Tyr Thr Leu Glu Glu Lys Asn Glu Leu Thr Asn
                725                 730                 735     
Lys Tyr Asp Ile Lys Gln Ile Glu Asn Glu Leu Asn Gln Lys Val Ser
            740                 745                 750         
Ile Ala Met Asn Asn Ile Asp Arg Phe Leu Thr Glu Ser Ser Ile Ser
        755                 760                 765             
Tyr Leu Met Lys Leu Ile Asn Glu Val Lys Ile Asn Lys Leu Arg Glu
    770                 775                 780                 
Tyr Asp Glu Asn Val Lys Thr Tyr Leu Leu Asn Tyr Ile Ile Gln His
785                 790                 795                 800 
Gly Ser Ile Leu Gly Glu Ser Gln Gln Glu Leu Asn Ser Met Val Thr
                805                 810                 815     
Asp Thr Leu Asn Asn Ser Ile Pro Phe Lys Leu Ser Ser Tyr Thr Asp
            820                 825                 830         
Asp Lys Ile Leu Ile Ser Tyr Phe Asn Lys Phe Phe Lys Arg Ile Lys
        835                 840                 845             
Ser Ser Ser Val Leu Asn Met Arg Tyr Lys Asn Asp Lys Tyr Val Asp
    850                 855                 860                 
Thr Ser Gly Tyr Asp Ser Asn Ile Asn Ile Asn Gly Asp Val Tyr Lys
865                 870                 875                 880 
Tyr Pro Thr Asn Lys Asn Gln Phe Gly Ile Tyr Asn Asp Lys Leu Ser
                885                 890                 895     
Glu Val Asn Ile Ser Gln Asn Asp Tyr Ile Ile Tyr Asp Asn Lys Tyr
            900                 905                 910         
Lys Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro Asn Tyr Asp Asn
        915                 920                 925             
Lys Ile Val Asn Val Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Arg
    930                 935                 940                 
Asp Asn Asn Ser Gly Trp Lys Val Ser Leu Asn His Asn Glu Ile Ile
945                 950                 955                 960 
Trp Thr Leu Gln Asp Asn Ala Gly Ile Asn Gln Lys Leu Ala Phe Asn
                965                 970                 975     
Tyr Gly Asn Ala Asn Gly Ile Ser Asp Tyr Ile Asn Lys Trp Ile Phe
            980                 985                 990         
Val Thr Ile Thr Asn Asp Arg Leu Gly Asp Ser Lys Leu Tyr Ile Asn
        995                 1000                1005            
Gly Asn Leu Ile Asp Gln Lys Ser Ile Leu Asn Leu Gly Asn Ile His
    1010                1015                1020                
Val Ser Asp Asn Ile Leu Phe Lys Ile Val Asn Cys Ser Tyr Thr Arg
1025                1030                1035                1040
Tyr Ile Gly Ile Arg Tyr Phe Asn Ile Phe Asp Lys Glu Leu Asp Glu
                1045                1050                1055    
Thr Glu Ile Gln Thr Leu Tyr Ser Asn Glu Pro Asn Thr Asn Ile Leu
            1060                1065                1070        
Lys Asp Phe Trp Gly Asn Tyr Leu Leu Tyr Asp Lys Glu Tyr Tyr Leu
        1075                1080                1085            
Leu Asn Val Leu Lys Pro Asn Asn Phe Ile Asp Arg Arg Lys Asp Ser
    1090                1095                1100                
Thr Leu Ser Ile Asn Asn Ile Arg Ser Thr Ile Leu Leu Ala Asn Arg
1105                1110                1115                1120
Leu Tyr Ser Gly Ile Lys Val Lys Ile Gln Arg Val Asn Asn Ser Ser
                1125                1130                1135    
Thr Asn Asp Asn Leu Val Arg Lys Asn Asp Gln Val Tyr Ile Asn Phe
            1140                1145                1150        
Val Ala Ser Lys Thr His Leu Phe Pro Leu Tyr Ala Asp Thr Ala Thr
        1155                1160                1165            
Thr Asn Lys Glu Lys Thr Ile Lys Ile Ser Ser Ser Gly Asn Arg Phe
    1170                1175                1180                
Asn Gln Val Val Val Met Asn Ser Val Gly Asn Asn Cys Thr Met Asn
1185                1190                1195                1200
Phe Lys Asn Asn Asn Gly Asn Asn Ile Gly Leu Leu Gly Phe Lys Ala
                1205                1210                1215    
Asp Thr Val Val Ala Ser Thr Trp Tyr Tyr Thr His Met Arg Asp His
            1220                1225                1230        
Thr Asn Ser Asn Gly Cys Phe Trp Asn Phe Ile Ser Glu Glu His Gly
        1235                1240                1245            
Trp Gln Glu Lys
    1250        


<210> 6
<211> 1274
<212> PRT
<213> Clostridium botulinum Serotype F

<400> 6
Met Pro Val Ala Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp
 1               5                  10                  15      
Asp Thr Ile Leu Tyr Met Gln Ile Pro Tyr Glu Glu Lys Ser Lys Lys
            20                  25                  30          
Tyr Tyr Lys Ala Phe Glu Ile Met Arg Asn Val Trp Ile Ile Pro Glu
        35                  40                  45              
Arg Asn Thr Ile Gly Thr Asn Pro Ser Asp Phe Asp Pro Pro Ala Ser
    50                  55                  60                  
Leu Lys Asn Gly Ser Ser Ala Tyr Tyr Asp Pro Asn Tyr Leu Thr Thr
65                  70                  75                  80  
Asp Ala Glu Lys Asp Arg Tyr Leu Lys Thr Thr Ile Lys Leu Phe Lys
                85                  90                  95      
Arg Ile Asn Ser Asn Pro Ala Gly Lys Val Leu Leu Gln Glu Ile Ser
            100                 105                 110         
Tyr Ala Lys Pro Tyr Leu Gly Asn Asp His Thr Pro Ile Asp Glu Phe
        115                 120                 125             
Ser Pro Val Thr Arg Thr Thr Ser Val Asn Ile Lys Leu Ser Thr Asn
    130                 135                 140                 
Val Glu Ser Ser Met Leu Leu Asn Leu Leu Val Leu Gly Ala Gly Pro
145                 150                 155                 160 
Asp Ile Phe Glu Ser Cys Cys Tyr Pro Val Arg Lys Leu Ile Asp Pro
                165                 170                 175     
Asp Val Val Tyr Asp Pro Ser Asn Tyr Gly Phe Gly Ser Ile Asn Ile
            180                 185                 190         
Val Thr Phe Ser Pro Glu Tyr Glu Tyr Thr Phe Asn Asp Ile Ser Gly
        195                 200                 205             
Gly His Asn Ser Ser Thr Glu Ser Phe Ile Ala Asp Pro Ala Ile Ser
    210                 215                 220                 
Leu Ala His Glu Leu Ile His Ala Leu His Gly Leu Tyr Gly Ala Arg
225                 230                 235                 240 
Gly Val Thr Tyr Glu Glu Thr Ile Glu Val Lys Gln Ala Pro Leu Met
                245                 250                 255     
Ile Ala Glu Lys Pro Ile Arg Leu Glu Glu Phe Leu Thr Phe Gly Gly
            260                 265                 270         
Gln Asp Leu Asn Ile Ile Thr Ser Ala Met Lys Glu Lys Ile Tyr Asn
        275                 280                 285             
Asn Leu Leu Ala Asn Tyr Glu Lys Ile Ala Thr Arg Leu Ser Glu Val
    290                 295                 300                 
Asn Ser Ala Pro Pro Glu Tyr Asp Ile Asn Glu Tyr Lys Asp Tyr Phe
305                 310                 315                 320 
Gln Trp Lys Tyr Gly Leu Asp Lys Asn Ala Asp Gly Ser Tyr Thr Val
                325                 330                 335     
Asn Glu Asn Lys Phe Asn Glu Ile Tyr Lys Lys Leu Tyr Ser Phe Thr
            340                 345                 350         
Glu Ser Asp Leu Ala Asn Lys Phe Lys Val Lys Cys Arg Asn Thr Tyr
        355                 360                 365             
Phe Ile Lys Tyr Glu Phe Leu Lys Val Pro Asn Leu Leu Asp Asp Asp
    370                 375                 380                 
Ile Tyr Thr Val Ser Glu Gly Phe Asn Ile Gly Asn Leu Ala Val Asn
385                 390                 395                 400 
Asn Arg Gly Gln Ser Ile Lys Leu Asn Pro Lys Ile Ile Asp Ser Ile
                405                 410                 415     
Pro Asp Lys Gly Leu Val Glu Lys Ile Val Lys Phe Cys Lys Ser Val
            420                 425                 430         
Ile Pro Arg Lys Gly Thr Lys Ala Pro Pro Arg Leu Cys Ile Arg Val
        435                 440                 445             
Asn Asn Ser Glu Leu Phe Phe Val Ala Ser Glu Ser Ser Tyr Asn Glu
    450                 455                 460                 
Asn Asp Ile Asn Thr Pro Lys Glu Ile Asp Asp Thr Thr Asn Leu Asn
465                 470                 475                 480 
Asn Asn Tyr Arg Asn Asn Leu Asp Glu Val Ile Leu Asp Tyr Asn Ser
                485                 490                 495     
Gln Thr Ile Pro Gln Ile Ser Asn Arg Thr Leu Asn Thr Leu Val Gln
            500                 505                 510         
Asp Asn Ser Tyr Val Pro Arg Tyr Asp Ser Asn Gly Thr Ser Glu Ile
        515                 520                 525             
Glu Glu Tyr Asp Val Val Asp Phe Asn Val Phe Phe Tyr Leu His Ala
    530                 535                 540                 
Gln Lys Val Pro Glu Gly Glu Thr Asn Ile Ser Leu Thr Ser Ser Ile
545                 550                 555                 560 
Asp Thr Ala Leu Leu Glu Glu Ser Lys Asp Ile Phe Phe Ser Ser Glu
                565                 570                 575     
Phe Ile Asp Thr Ile Asn Lys Pro Val Asn Ala Ala Leu Phe Ile Asp
            580                 585                 590         
Trp Ile Ser Lys Val Ile Arg Asp Phe Thr Thr Glu Ala Thr Gln Lys
        595                 600                 605             
Ser Thr Val Asp Lys Ile Ala Asp Ile Ser Leu Ile Val Pro Tyr Val
    610                 615                 620                 
Gly Leu Ala Leu Asn Ile Ile Ile Glu Ala Glu Lys Gly Asn Phe Glu
625                 630                 635                 640 
Glu Ala Phe Glu Leu Leu Gly Val Gly Ile Leu Leu Glu Phe Val Pro
                645                 650                 655     
Glu Leu Thr Ile Pro Val Ile Leu Val Phe Thr Ile Lys Ser Tyr Ile
            660                 665                 670         
Asp Ser Tyr Glu Asn Lys Asn Lys Ala Ile Lys Ala Ile Asn Asn Ser
        675                 680                 685             
Leu Ile Glu Arg Glu Ala Lys Trp Lys Glu Ile Tyr Ser Trp Ile Val
    690                 695                 700                 
Ser Asn Trp Leu Thr Arg Ile Asn Thr Gln Phe Asn Lys Arg Lys Glu
705                 710                 715                 720 
Gln Met Tyr Gln Ala Leu Gln Asn Gln Val Asp Ala Ile Lys Thr Ala
                725                 730                 735     
Ile Glu Tyr Lys Tyr Asn Asn Tyr Thr Ser Asp Glu Lys Asn Arg Leu
            740                 745                 750         
Glu Ser Glu Tyr Asn Ile Asn Asn Ile Glu Glu Glu Leu Asn Lys Lys
        755                 760                 765             
Val Ser Leu Ala Met Lys Asn Ile Glu Arg Phe Met Thr Glu Ser Ser
    770                 775                 780                 
Ile Ser Tyr Leu Met Lys Leu Ile Asn Glu Ala Lys Val Gly Lys Leu
785                 790                 795                 800 
Lys Lys Tyr Asp Asn His Val Lys Ser Asp Leu Leu Asn Tyr Ile Leu
                805                 810                 815     
Asp His Arg Ser Ile Leu Gly Glu Gln Thr Asn Glu Leu Ser Asp Leu
            820                 825                 830         
Val Thr Ser Thr Leu Asn Ser Ser Ile Pro Phe Glu Leu Ser Ser Tyr
        835                 840                 845             
Thr Asn Asp Lys Ile Leu Ile Ile Tyr Phe Asn Arg Leu Tyr Lys Lys
    850                 855                 860                 
Ile Lys Asp Ser Ser Ile Leu Asp Met Arg Tyr Glu Asn Asn Lys Phe
865                 870                 875                 880 
Ile Asp Ile Ser Gly Tyr Gly Ser Asn Ile Ser Ile Asn Gly Asn Val
                885                 890                 895     
Tyr Ile Tyr Ser Thr Asn Arg Asn Gln Phe Gly Ile Tyr Asn Ser Arg
            900                 905                 910         
Leu Ser Glu Val Asn Ile Ala Gln Asn Asn Asp Ile Ile Tyr Asn Ser
        915                 920                 925             
Arg Tyr Gln Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro Lys His
    930                 935                 940                 
Tyr Lys Pro Met Asn His Asn Arg Glu Tyr Thr Ile Ile Asn Cys Met
945                 950                 955                 960 
Gly Asn Asn Asn Ser Gly Trp Lys Ile Ser Leu Arg Thr Val Arg Asp
                965                 970                 975     
Cys Glu Ile Ile Trp Thr Leu Gln Asp Thr Ser Gly Asn Lys Glu Asn
            980                 985                 990         
Leu Ile Phe Arg Tyr Glu Glu Leu Asn Arg Ile Ser Asn Tyr Ile Asn
        995                 1000                1005            
Lys Trp Ile Phe Val Thr Ile Thr Asn Asn Arg Leu Gly Asn Ser Arg
    1010                1015                1020                
Ile Tyr Ile Asn Gly Asn Leu Ile Val Glu Lys Ser Ile Ser Asn Leu
1025                1030                1035                1040
Gly Asp Ile His Val Ser Asp Asn Ile Leu Phe Lys Ile Val Gly Cys
                1045                1050                1055    
Asp Asp Glu Thr Tyr Val Gly Ile Arg Tyr Phe Lys Val Phe Asn Thr
            1060                1065                1070        
Glu Leu Asp Lys Thr Glu Ile Glu Thr Leu Tyr Ser Asn Glu Pro Asp
        1075                1080                1085            
Pro Ser Ile Leu Lys Asn Tyr Trp Gly Asn Tyr Leu Leu Tyr Asn Lys
    1090                1095                1100                
Lys Tyr Tyr Leu Phe Asn Leu Leu Arg Lys Asp Lys Tyr Ile Thr Leu
1105                1110                1115                1120
Asn Ser Gly Ile Leu Asn Ile Asn Gln Gln Arg Gly Val Thr Glu Gly
                1125                1130                1135    
Ser Val Phe Leu Asn Tyr Lys Leu Tyr Glu Gly Val Glu Val Ile Ile
            1140                1145                1150        
Arg Lys Asn Gly Pro Ile Asp Ile Ser Asn Thr Asp Asn Phe Val Arg
        1155                1160                1165            
Lys Asn Asp Leu Ala Tyr Ile Asn Val Val Asp Arg Gly Val Glu Tyr
    1170                1175                1180                
Arg Leu Tyr Ala Asp Thr Lys Ser Glu Lys Glu Lys Ile Ile Arg Thr
1185                1190                1195                1200
Ser Asn Leu Asn Asp Ser Leu Gly Gln Ile Ile Val Met Asp Ser Ile
                1205                1210                1215    
Gly Asn Asn Cys Thr Met Asn Phe Gln Asn Asn Asn Gly Ser Asn Ile
            1220                1225                1230        
Gly Leu Leu Gly Phe His Ser Asn Asn Leu Val Ala Ser Ser Trp Tyr
        1235                1240                1245            
Tyr Asn Asn Ile Arg Arg Asn Thr Ser Ser Asn Gly Cys Phe Trp Ser
    1250                1255                1260                
Ser Ile Ser Lys Glu Asn Gly Trp Lys Glu
1265                1270                


<210> 7
<211> 1297
<212> PRT
<213> Clostridium botulinum Serotype G

<400> 7
Met Pro Val Asn Ile Lys Asn Phe Asn Tyr Asn Asp Pro Ile Asn Asn
 1               5                  10                  15      
Asp Asp Ile Ile Met Met Glu Pro Phe Asn Asp Pro Gly Pro Gly Thr
            20                  25                  30          
Tyr Tyr Lys Ala Phe Arg Ile Ile Asp Arg Ile Trp Ile Val Pro Glu
        35                  40                  45              
Arg Phe Thr Tyr Gly Phe Gln Pro Asp Gln Phe Asn Ala Ser Thr Gly
    50                  55                  60                  
Val Phe Ser Lys Asp Val Tyr Glu Tyr Tyr Asp Pro Thr Tyr Leu Lys
65                  70                  75                  80  
Thr Asp Ala Glu Lys Asp Lys Phe Leu Lys Thr Met Ile Lys Leu Phe
                85                  90                  95      
Asn Arg Ile Asn Ser Lys Pro Ser Gly Gln Arg Leu Leu Asp Met Ile
            100                 105                 110         
Val Asp Ala Ile Pro Tyr Leu Gly Asn Ala Ser Thr Pro Pro Asp Lys
        115                 120                 125             
Phe Ala Ala Asn Val Ala Asn Val Ser Ile Asn Lys Lys Ile Ile Gln
    130                 135                 140                 
Pro Gly Ala Glu Asp Gln Ile Lys Gly Leu Met Thr Asn Leu Ile Ile
145                 150                 155                 160 
Phe Gly Pro Gly Pro Val Leu Ser Asp Asn Phe Thr Asp Ser Met Ile
                165                 170                 175     
Met Asn Gly His Ser Pro Ile Ser Glu Gly Phe Gly Ala Arg Met Met
            180                 185                 190         
Ile Arg Phe Cys Pro Ser Cys Leu Asn Val Phe Asn Asn Val Gln Glu
        195                 200                 205             
Asn Lys Asp Thr Ser Ile Phe Ser Arg Arg Ala Tyr Phe Ala Asp Pro
    210                 215                 220                 
Ala Leu Thr Leu Met His Glu Leu Ile His Val Leu His Gly Leu Tyr
225                 230                 235                 240 
Gly Ile Lys Ile Ser Asn Leu Pro Ile Thr Pro Asn Thr Lys Glu Phe
                245                 250                 255     
Phe Met Gln His Ser Asp Pro Val Gln Ala Glu Glu Leu Tyr Thr Phe
            260                 265                 270         
Gly Gly His Asp Pro Ser Val Ile Ser Pro Ser Thr Asp Met Asn Ile
        275                 280                 285             
Tyr Asn Lys Ala Leu Gln Asn Phe Gln Asp Ile Ala Asn Arg Leu Asn
    290                 295                 300                 
Ile Val Ser Ser Ala Gln Gly Ser Gly Ile Asp Ile Ser Leu Tyr Lys
305                 310                 315                 320 
Gln Ile Tyr Lys Asn Lys Tyr Asp Phe Val Glu Asp Pro Asn Gly Lys
                325                 330                 335     
Tyr Ser Val Asp Lys Asp Lys Phe Asp Lys Leu Tyr Lys Ala Leu Met
            340                 345                 350         
Phe Gly Phe Thr Glu Thr Asn Leu Ala Gly Glu Tyr Gly Ile Lys Thr
        355                 360                 365             
Arg Tyr Ser Tyr Phe Ser Glu Tyr Leu Pro Pro Ile Lys Thr Glu Lys
    370                 375                 380                 
Leu Leu Asp Asn Thr Ile Tyr Thr Gln Asn Glu Gly Phe Asn Ile Ala
385                 390                 395                 400 
Ser Lys Asn Leu Lys Thr Glu Phe Asn Gly Gln Asn Lys Ala Val Asn
                405                 410                 415     
Lys Glu Ala Tyr Glu Glu Ile Ser Leu Glu His Leu Val Ile Tyr Arg
            420                 425                 430         
Ile Ala Met Cys Lys Pro Val Met Tyr Lys Asn Thr Gly Lys Ser Glu
        435                 440                 445             
Gln Cys Ile Ile Val Asn Asn Glu Asp Leu Phe Phe Ile Ala Asn Lys
    450                 455                 460                 
Asp Ser Phe Ser Lys Asp Leu Ala Lys Ala Glu Thr Ile Ala Tyr Asn
465                 470                 475                 480 
Thr Gln Asn Asn Thr Ile Glu Asn Asn Phe Ser Ile Asp Gln Leu Ile
                485                 490                 495     
Leu Asp Asn Asp Leu Ser Ser Gly Ile Asp Leu Pro Asn Glu Asn Thr
            500                 505                 510         
Glu Pro Phe Thr Asn Phe Asp Asp Ile Asp Ile Pro Val Tyr Ile Lys
        515                 520                 525             
Gln Ser Ala Leu Lys Lys Ile Phe Val Asp Gly Asp Ser Leu Phe Glu
    530                 535                 540                 
Tyr Leu His Ala Gln Thr Phe Pro Ser Asn Ile Glu Asn Leu Gln Leu
545                 550                 555                 560 
Thr Asn Ser Leu Asn Asp Ala Leu Arg Asn Asn Asn Lys Val Tyr Thr
                565                 570                 575     
Phe Phe Ser Thr Asn Leu Val Glu Lys Ala Asn Thr Val Val Gly Ala
            580                 585                 590         
Ser Leu Phe Val Asn Trp Val Lys Gly Val Ile Asp Asp Phe Thr Ser
        595                 600                 605             
Glu Ser Thr Gln Lys Ser Thr Ile Asp Lys Val Ser Asp Val Ser Ile
    610                 615                 620                 
Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Val Gly Asn Glu Thr Ala
625                 630                 635                 640 
Lys Glu Asn Phe Lys Asn Ala Phe Glu Ile Gly Gly Ala Ala Ile Leu
                645                 650                 655     
Met Glu Phe Ile Pro Glu Leu Ile Val Pro Ile Val Gly Phe Phe Thr
            660                 665                 670         
Leu Glu Ser Tyr Val Gly Asn Lys Gly His Ile Ile Met Thr Ile Ser
        675                 680                 685             
Asn Ala Leu Lys Lys Arg Asp Gln Lys Trp Thr Asp Met Tyr Gly Leu
    690                 695                 700                 
Ile Val Ser Gln Trp Leu Ser Thr Val Asn Thr Gln Phe Tyr Thr Ile
705                 710                 715                 720 
Lys Glu Arg Met Tyr Asn Ala Leu Asn Asn Gln Ser Gln Ala Ile Glu
                725                 730                 735     
Lys Ile Ile Glu Asp Gln Tyr Asn Arg Tyr Ser Glu Glu Asp Lys Met
            740                 745                 750         
Asn Ile Asn Ile Asp Phe Asn Asp Ile Asp Phe Lys Leu Asn Gln Ser
        755                 760                 765             
Ile Asn Leu Ala Ile Asn Asn Ile Asp Asp Phe Ile Asn Gln Cys Ser
    770                 775                 780                 
Ile Ser Tyr Leu Met Asn Arg Met Ile Pro Leu Ala Val Lys Lys Leu
785                 790                 795                 800 
Lys Asp Phe Asp Asp Asn Leu Lys Arg Asp Leu Leu Glu Tyr Ile Asp
                805                 810                 815     
Thr Asn Glu Leu Tyr Leu Leu Asp Glu Val Asn Ile Leu Lys Ser Lys
            820                 825                 830         
Val Asn Arg His Leu Lys Asp Ser Ile Pro Phe Asp Leu Ser Leu Tyr
        835                 840                 845             
Thr Lys Asp Thr Ile Leu Ile Gln Val Phe Asn Asn Tyr Ile Ser Asn
    850                 855                 860                 
Ile Ser Ser Asn Ala Ile Leu Ser Leu Ser Tyr Arg Gly Gly Arg Leu
865                 870                 875                 880 
Ile Asp Ser Ser Gly Tyr Gly Ala Thr Met Asn Val Gly Ser Asp Val
                885                 890                 895     
Ile Phe Asn Asp Ile Gly Asn Gly Gln Phe Lys Leu Asn Asn Ser Glu
            900                 905                 910         
Asn Ser Asn Ile Thr Ala His Gln Ser Lys Phe Val Val Tyr Asp Ser
        915                 920                 925             
Met Phe Asp Asn Phe Ser Ile Asn Phe Trp Val Arg Thr Pro Lys Tyr
    930                 935                 940                 
Asn Asn Asn Asp Ile Gln Thr Tyr Leu Gln Asn Glu Tyr Thr Ile Ile
945                 950                 955                 960 
Ser Cys Ile Lys Asn Asp Ser Gly Trp Lys Val Ser Ile Lys Gly Asn
                965                 970                 975     
Arg Ile Ile Trp Thr Leu Ile Asp Val Asn Ala Lys Ser Lys Ser Ile
            980                 985                 990         
Phe Phe Glu Tyr Ser Ile Lys Asp Asn Ile Ser Asp Tyr Ile Asn Lys
        995                 1000                1005            
Trp Phe Ser Ile Thr Ile Thr Asn Asp Arg Leu Gly Asn Ala Asn Ile
    1010                1015                1020                
Tyr Ile Asn Gly Ser Leu Lys Lys Ser Glu Lys Ile Leu Asn Leu Asp
1025                1030                1035                1040
Arg Ile Asn Ser Ser Asn Asp Ile Asp Phe Lys Leu Ile Asn Cys Thr
                1045                1050                1055    
Asp Thr Thr Lys Phe Val Trp Ile Lys Asp Phe Asn Ile Phe Gly Arg
            1060                1065                1070        
Glu Leu Asn Ala Thr Glu Val Ser Ser Leu Tyr Trp Ile Gln Ser Ser
        1075                1080                1085            
Thr Asn Thr Leu Lys Asp Phe Trp Gly Asn Pro Leu Arg Tyr Asp Thr
    1090                1095                1100                
Gln Tyr Tyr Leu Phe Asn Gln Gly Met Gln Asn Ile Tyr Ile Lys Tyr
1105                1110                1115                1120
Phe Ser Lys Ala Ser Met Gly Glu Thr Ala Pro Arg Thr Asn Phe Asn
                1125                1130                1135    
Asn Ala Ala Ile Asn Tyr Gln Asn Leu Tyr Leu Gly Leu Arg Phe Ile
            1140                1145                1150        
Ile Lys Lys Ala Ser Asn Ser Arg Asn Ile Asn Asn Asp Asn Ile Val
        1155                1160                1165            
Arg Glu Gly Asp Tyr Ile Tyr Leu Asn Ile Asp Asn Ile Ser Asp Glu
    1170                1175                1180                
Ser Tyr Arg Val Tyr Val Leu Val Asn Ser Lys Glu Ile Gln Thr Gln
1185                1190                1195                1200
Leu Phe Leu Ala Pro Ile Asn Asp Asp Pro Thr Phe Tyr Asp Val Leu
                1205                1210                1215    
Gln Ile Lys Lys Tyr Tyr Glu Lys Thr Thr Tyr Asn Cys Gln Ile Leu
            1220                1225                1230        
Cys Glu Lys Asp Thr Lys Thr Phe Gly Leu Phe Gly Ile Gly Lys Phe
        1235                1240                1245            
Val Lys Asp Tyr Gly Tyr Val Trp Asp Thr Tyr Asp Asn Tyr Phe Cys
    1250                1255                1260                
Ile Ser Gln Trp Tyr Leu Arg Arg Ile Ser Glu Asn Ile Asn Lys Leu
1265                1270                1275                1280
Arg Leu Gly Cys Asn Trp Gln Phe Ile Pro Val Asp Glu Gly Trp Thr
                1285                1290                1295    
Glu
    


<210> 8
<211> 1315
<212> PRT
<213> Clostridium tetani

<400> 8
Met Pro Ile Thr Ile Asn Asn Phe Arg Tyr Ser Asp Pro Val Asn Asn
 1               5                  10                  15      
Asp Thr Ile Ile Met Met Glu Pro Pro Tyr Cys Lys Gly Leu Asp Ile
            20                  25                  30          
Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Val Pro Glu
        35                  40                  45              
Arg Tyr Glu Phe Gly Thr Lys Pro Glu Asp Phe Asn Pro Pro Ser Ser
    50                  55                  60                  
Leu Ile Glu Gly Ala Ser Glu Tyr Tyr Asp Pro Asn Tyr Leu Arg Thr
65                  70                  75                  80  
Asp Ser Asp Lys Asp Arg Phe Leu Gln Thr Met Val Lys Leu Phe Asn
                85                  90                  95      
Arg Ile Lys Asn Asn Val Ala Gly Glu Ala Leu Leu Asp Lys Ile Ile
            100                 105                 110         
Asn Ala Ile Pro Tyr Leu Gly Asn Ser Tyr Ser Leu Leu Asp Lys Phe
        115                 120                 125             
Asp Thr Asn Ser Asn Ser Val Ser Phe Asn Leu Leu Glu Gln Asp Pro
    130                 135                 140                 
Ser Gly Ala Thr Thr Lys Ser Ala Met Leu Thr Asn Leu Ile Ile Phe
145                 150                 155                 160 
Gly Pro Gly Pro Val Leu Asn Lys Asn Glu Val Arg Gly Ile Val Leu
                165                 170                 175     
Arg Val Asp Asn Lys Asn Tyr Phe Pro Cys Arg Asp Gly Phe Gly Ser
            180                 185                 190         
Ile Met Gln Met Ala Phe Cys Pro Glu Tyr Val Pro Thr Phe Asp Asn
        195                 200                 205             
Val Ile Glu Asn Ile Thr Ser Leu Thr Ile Gly Lys Ser Lys Tyr Phe
    210                 215                 220                 
Gln Asp Pro Ala Leu Leu Leu Met His Glu Leu Ile His Val Leu His
225                 230                 235                 240 
Gly Leu Tyr Gly Met Gln Val Ser Ser His Glu Ile Ile Pro Ser Lys
                245                 250                 255     
Gln Glu Ile Tyr Met Gln His Thr Tyr Pro Ile Ser Ala Glu Glu Leu
            260                 265                 270         
Phe Thr Phe Gly Gly Gln Asp Ala Asn Leu Ile Ser Ile Asp Ile Lys
        275                 280                 285             
Asn Asp Leu Tyr Glu Lys Thr Leu Asn Asp Tyr Lys Ala Ile Ala Asn
    290                 295                 300                 
Lys Leu Ser Gln Val Thr Ser Cys Asn Asp Pro Asn Ile Asp Ile Asp
305                 310                 315                 320 
Ser Tyr Lys Gln Ile Tyr Gln Gln Lys Tyr Gln Phe Asp Lys Asp Ser
                325                 330                 335     
Asn Gly Gln Tyr Ile Val Asn Glu Asp Lys Phe Gln Ile Leu Tyr Asn
            340                 345                 350         
Ser Ile Met Tyr Gly Phe Thr Glu Ile Glu Leu Gly Lys Lys Phe Asn
        355                 360                 365             
Ile Lys Thr Arg Leu Ser Tyr Phe Ser Met Asn His Asp Pro Val Lys
    370                 375                 380                 
Ile Pro Asn Leu Leu Asp Asp Thr Ile Tyr Asn Asp Thr Glu Gly Phe
385                 390                 395                 400 
Asn Ile Glu Ser Lys Asp Leu Lys Ser Glu Tyr Lys Gly Gln Asn Met
                405                 410                 415     
Arg Val Asn Thr Asn Ala Phe Arg Asn Val Asp Gly Ser Gly Leu Val
            420                 425                 430         
Ser Lys Leu Ile Gly Leu Cys Lys Lys Ile Ile Pro Pro Thr Asn Ile
        435                 440                 445             
Arg Glu Asn Leu Tyr Asn Arg Thr Ala Ser Leu Thr Asp Leu Gly Gly
    450                 455                 460                 
Glu Leu Cys Ile Lys Ile Lys Asn Glu Asp Leu Thr Phe Ile Ala Glu
465                 470                 475                 480 
Lys Asn Ser Phe Ser Glu Glu Pro Phe Gln Asp Glu Ile Val Ser Tyr
                485                 490                 495     
Asn Thr Lys Asn Lys Pro Leu Asn Phe Asn Tyr Ser Leu Asp Lys Ile
            500                 505                 510         
Ile Val Asp Tyr Asn Leu Gln Ser Lys Ile Thr Leu Pro Asn Asp Arg
        515                 520                 525             
Thr Thr Pro Val Thr Lys Gly Ile Pro Tyr Ala Pro Glu Tyr Lys Ser
    530                 535                 540                 
Asn Ala Ala Ser Thr Ile Glu Ile His Asn Ile Asp Asp Asn Thr Ile
545                 550                 555                 560 
Tyr Gln Tyr Leu Tyr Ala Gln Lys Ser Pro Thr Thr Leu Gln Arg Ile
                565                 570                 575     
Thr Met Thr Asn Ser Val Asp Asp Ala Leu Ile Asn Ser Thr Lys Ile
            580                 585                 590         
Tyr Ser Tyr Phe Pro Ser Val Ile Ser Lys Val Asn Gln Gly Ala Gln
        595                 600                 605             
Gly Ile Leu Phe Leu Gln Trp Val Arg Asp Ile Ile Asp Asp Phe Thr
    610                 615                 620                 
Asn Glu Ser Ser Gln Lys Thr Thr Ile Asp Lys Ile Ser Asp Val Ser
625                 630                 635                 640 
Thr Ile Val Pro Tyr Ile Gly Pro Ala Leu Asn Ile Val Lys Gln Gly
                645                 650                 655     
Tyr Glu Gly Asn Phe Ile Gly Ala Leu Glu Thr Thr Gly Val Val Leu
            660                 665                 670         
Leu Leu Glu Tyr Ile Pro Glu Ile Thr Leu Pro Val Ile Ala Ala Leu
        675                 680                 685             
Ser Ile Ala Glu Ser Ser Thr Gln Lys Glu Lys Ile Ile Lys Thr Ile
    690                 695                 700                 
Asp Asn Phe Leu Glu Lys Arg Tyr Glu Lys Trp Ile Glu Val Tyr Lys
705                 710                 715                 720 
Leu Val Lys Ala Lys Trp Leu Gly Thr Val Asn Thr Gln Phe Gln Lys
                725                 730                 735     
Arg Ser Tyr Gln Met Tyr Arg Ser Leu Glu Tyr Gln Val Asp Ala Ile
            740                 745                 750         
Lys Lys Ile Ile Asp Tyr Glu Tyr Lys Ile Tyr Ser Gly Pro Asp Lys
        755                 760                 765             
Glu Gln Ile Ala Asp Glu Ile Asn Asn Leu Lys Asn Lys Leu Glu Glu
    770                 775                 780                 
Lys Ala Asn Lys Ala Met Ile Asn Ile Asn Ile Phe Met Arg Glu Ser
785                 790                 795                 800 
Ser Arg Ser Phe Leu Val Asn Gln Met Ile Asn Glu Ala Lys Lys Gln
                805                 810                 815     
Leu Leu Glu Phe Asp Thr Gln Ser Lys Asn Ile Leu Met Gln Tyr Ile
            820                 825                 830         
Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Glu Leu Lys Lys Leu Glu
        835                 840                 845             
Ser Lys Ile Asn Lys Val Phe Ser Thr Pro Ile Pro Phe Ser Tyr Ser
    850                 855                 860                 
Lys Asn Leu Asp Cys Trp Val Asp Asn Glu Glu Asp Ile Asp Val Ile
865                 870                 875                 880 
Leu Lys Lys Ser Thr Ile Leu Asn Leu Asp Ile Asn Asn Asp Ile Ile
                885                 890                 895     
Ser Asp Ile Ser Gly Phe Asn Ser Ser Val Ile Thr Tyr Pro Asp Ala
            900                 905                 910         
Gln Leu Val Pro Gly Ile Asn Gly Lys Ala Ile His Leu Val Asn Asn
        915                 920                 925             
Glu Ser Ser Glu Val Ile Val His Lys Ala Met Asp Ile Glu Tyr Asn
    930                 935                 940                 
Asp Met Phe Asn Asn Phe Thr Val Ser Phe Trp Leu Arg Val Pro Lys
945                 950                 955                 960 
Val Ser Ala Ser His Leu Glu Gln Tyr Gly Thr Asn Glu Tyr Ser Ile
                965                 970                 975     
Ile Ser Ser Met Lys Lys His Ser Leu Ser Ile Gly Ser Gly Trp Ser
            980                 985                 990         
Val Ser Leu Lys Gly Asn Asn Leu Ile Trp Thr Leu Lys Asp Ser Ala
        995                 1000                1005            
Gly Glu Val Arg Gln Ile Thr Phe Arg Asp Leu Pro Asp Lys Phe Asn
    1010                1015                1020                
Ala Tyr Leu Ala Asn Lys Trp Val Phe Ile Thr Ile Thr Asn Asp Arg
1025                1030                1035                1040
Leu Ser Ser Ala Asn Leu Tyr Ile Asn Gly Val Leu Met Gly Ser Ala
                1045                1050                1055    
Glu Ile Thr Gly Leu Gly Ala Ile Arg Glu Asp Asn Asn Ile Thr Leu
            1060                1065                1070        
Lys Leu Asp Arg Cys Asn Asn Asn Asn Gln Tyr Val Ser Ile Asp Lys
        1075                1080                1085            
Phe Arg Ile Phe Cys Lys Ala Leu Asn Pro Lys Glu Ile Glu Lys Leu
    1090                1095                1100                
Tyr Thr Ser Tyr Leu Ser Ile Thr Phe Leu Arg Asp Phe Trp Gly Asn
1105                1110                1115                1120
Pro Leu Arg Tyr Asp Thr Glu Tyr Tyr Leu Ile Pro Val Ala Ser Ser
                1125                1130                1135    
Ser Lys Asp Val Gln Leu Lys Asn Ile Thr Asp Tyr Met Tyr Leu Thr
            1140                1145                1150        
Asn Ala Pro Ser Tyr Thr Asn Gly Lys Leu Asn Ile Tyr Tyr Arg Arg
        1155                1160                1165            
Leu Tyr Asn Gly Leu Lys Phe Ile Ile Lys Arg Tyr Thr Pro Asn Asn
    1170                1175                1180                
Glu Ile Asp Ser Phe Val Lys Ser Gly Asp Phe Ile Lys Leu Tyr Val
1185                1190                1195                1200
Ser Tyr Asn Asn Asn Glu His Ile Val Gly Tyr Pro Lys Asp Gly Asn
                1205                1210                1215    
Ala Phe Asn Asn Leu Asp Arg Ile Leu Arg Val Gly Tyr Asn Ala Pro
            1220                1225                1230        
Gly Ile Pro Leu Tyr Lys Lys Met Glu Ala Val Lys Leu Arg Asp Leu
        1235                1240                1245            
Lys Thr Tyr Ser Val Gln Leu Lys Leu Tyr Asp Asp Lys Asn Ala Ser
    1250                1255                1260                
Leu Gly Leu Val Gly Thr His Asn Gly Gln Ile Gly Asn Asp Pro Asn
1265                1270                1275                1280
Arg Asp Ile Leu Ile Ala Ser Asn Trp Tyr Phe Asn His Leu Lys Asp
                1285                1290                1295    
Lys Ile Leu Gly Cys Asp Trp Tyr Phe Val Pro Thr Asp Glu Gly Trp
            1300                1305                1310        
Thr Asn Asp
        1315


<210> 9
<211> 1268
<212> PRT
<213> Clostridium baratii

<400> 9
Met Pro Val Asn Ile Asn Asn Phe Asn Tyr Asn Asp Pro Ile Asn Asn
 1               5                  10                  15      
Thr Thr Ile Leu Tyr Met Lys Met Pro Tyr Tyr Glu Asp Ser Asn Lys
            20                  25                  30          
Tyr Tyr Lys Ala Phe Glu Ile Met Asp Asn Val Trp Ile Ile Pro Glu
        35                  40                  45              
Arg Asn Ile Ile Gly Lys Lys Pro Ser Asp Phe Tyr Pro Pro Ile Ser
    50                  55                  60                  
Leu Asp Ser Gly Ser Ser Ala Tyr Tyr Asp Pro Asn Tyr Leu Thr Thr
65                  70                  75                  80  
Asp Ala Glu Lys Asp Arg Phe Leu Lys Thr Val Ile Lys Leu Phe Asn
                85                  90                  95      
Arg Ile Asn Ser Asn Pro Ala Gly Gln Val Leu Leu Glu Glu Ile Lys
            100                 105                 110         
Asn Gly Lys Pro Tyr Leu Gly Asn Asp His Thr Ala Val Asn Glu Phe
        115                 120                 125             
Cys Ala Asn Asn Arg Ser Thr Ser Val Glu Ile Lys Glu Ser Asn Gly
    130                 135                 140                 
Thr Thr Asp Ser Met Leu Leu Asn Leu Val Ile Leu Gly Pro Gly Pro
145                 150                 155                 160 
Asn Ile Leu Glu Cys Ser Thr Phe Pro Val Arg Ile Phe Pro Asn Asn
                165                 170                 175     
Ile Ala Tyr Asp Pro Ser Glu Lys Gly Phe Gly Ser Ile Gln Leu Met
            180                 185                 190         
Ser Phe Ser Thr Glu Tyr Glu Tyr Ala Phe Asn Asp Asn Thr Asp Leu
        195                 200                 205             
Phe Ile Ala Asp Pro Ala Ile Ser Leu Ala His Glu Leu Ile His Val
    210                 215                 220                 
Leu His Gly Leu Tyr Gly Ala Lys Gly Val Thr Asn Lys Lys Val Ile
225                 230                 235                 240 
Glu Val Asp Gln Gly Ala Leu Met Ala Ala Glu Lys Asp Ile Lys Ile
                245                 250                 255     
Glu Glu Phe Ile Thr Phe Gly Gly Gln Asp Leu Asn Ile Ile Thr Asn
            260                 265                 270         
Ser Thr Asn Gln Lys Ile Tyr Val Ile Leu Leu Ser Asn Tyr Thr Ala
        275                 280                 285             
Ile Ala Ser Arg Leu Ser Gln Val Asn Arg Asn Asn Ser Ala Leu Asn
    290                 295                 300                 
Thr Thr Tyr Tyr Lys Asn Phe Phe Gln Trp Lys Tyr Gly Leu Asp Gln
305                 310                 315                 320 
Asp Ser Asn Gly Asn Tyr Thr Val Asn Ile Ser Lys Phe Asn Ala Ile
                325                 330                 335     
Tyr Lys Lys Leu Phe Ser Phe Thr Glu Cys Asp Leu Ala Gln Lys Phe
            340                 345                 350         
Gln Val Lys Asn Arg Ser Asn Tyr Leu Phe His Phe Lys Pro Phe Arg
        355                 360                 365             
Leu Leu Asp Leu Leu Asp Asp Asn Ile Tyr Ser Ile Ser Glu Gly Phe
    370                 375                 380                 
Asn Ile Gly Ser Leu Arg Val Asn Asn Asn Gly Gln Asn Ile Asn Leu
385                 390                 395                 400 
Asn Ser Arg Ile Val Gly Pro Ile Pro Asp Asn Gly Leu Val Glu Arg
                405                 410                 415     
Phe Val Gly Leu Cys Lys Ser Ile Val Ser Lys Lys Gly Thr Lys Asn
            420                 425                 430         
Ser Leu Cys Ile Lys Val Asn Asn Arg Asp Leu Phe Phe Val Ala Ser
        435                 440                 445             
Glu Ser Ser Tyr Asn Glu Asn Gly Ile Asn Ser Pro Lys Glu Ile Asp
    450                 455                 460                 
Asp Thr Thr Ile Thr Asn Asn Asn Tyr Lys Lys Asn Leu Asp Glu Val
465                 470                 475                 480 
Ile Leu Asp Tyr Asn Ser Asp Ala Ile Pro Asn Leu Ser Ser Arg Leu
                485                 490                 495     
Leu Asn Thr Thr Ala Gln Asn Asp Ser Tyr Val Pro Lys Tyr Asp Ser
            500                 505                 510         
Asn Gly Thr Ser Glu Ile Lys Glu Tyr Thr Val Asp Lys Leu Asn Val
        515                 520                 525             
Phe Phe Tyr Leu Tyr Ala Gln Lys Ala Pro Glu Gly Glu Ser Ala Ile
    530                 535                 540                 
Ser Leu Thr Ser Ser Val Asn Thr Ala Leu Leu Asp Ala Ser Lys Val
545                 550                 555                 560 
Tyr Thr Phe Phe Ser Ser Asp Phe Ile Asn Thr Val Asn Lys Pro Val
                565                 570                 575     
Gln Ala Ala Leu Phe Ile Ser Trp Ile Gln Gln Val Ile Asn Asp Phe
            580                 585                 590         
Thr Thr Glu Ala Thr Gln Lys Ser Thr Ile Asp Lys Ile Ala Asp Ile
        595                 600                 605             
Ser Leu Ile Val Pro Tyr Val Gly Leu Ala Leu Asn Ile Gly Asn Glu
    610                 615                 620                 
Val Gln Lys Gly Asn Phe Lys Glu Ala Ile Glu Leu Leu Gly Ala Gly
625                 630                 635                 640 
Ile Leu Leu Glu Phe Val Pro Glu Leu Leu Ile Pro Thr Ile Leu Val
                645                 650                 655     
Phe Thr Ile Lys Ser Phe Ile Asn Ser Asp Asp Ser Lys Asn Lys Ile
            660                 665                 670         
Ile Lys Ala Ile Asn Asn Ala Leu Arg Glu Arg Glu Leu Lys Trp Lys
        675                 680                 685             
Glu Val Tyr Ser Trp Ile Val Ser Asn Trp Leu Thr Arg Ile Asn Thr
    690                 695                 700                 
Gln Phe Asn Lys Arg Lys Glu Gln Met Tyr Gln Ala Leu Gln Asn Gln
705                 710                 715                 720 
Val Asp Gly Ile Lys Lys Ile Ile Glu Tyr Lys Tyr Asn Asn Tyr Thr
                725                 730                 735     
Leu Asp Glu Lys Asn Arg Leu Arg Ala Glu Tyr Asn Ile Tyr Ser Ile
            740                 745                 750         
Lys Glu Glu Leu Asn Lys Lys Val Ser Leu Ala Met Gln Asn Ile Asp
        755                 760                 765             
Arg Phe Leu Thr Glu Ser Ser Ile Ser Tyr Leu Met Lys Leu Ile Asn
    770                 775                 780                 
Glu Ala Lys Ile Asn Lys Leu Ser Glu Tyr Asp Lys Arg Val Asn Gln
785                 790                 795                 800 
Tyr Leu Leu Asn Tyr Ile Leu Glu Asn Ser Ser Thr Leu Gly Thr Ser
                805                 810                 815     
Ser Val Pro Glu Leu Asn Asn Leu Val Ser Asn Thr Leu Asn Asn Ser
            820                 825                 830         
Ile Pro Phe Glu Leu Ser Glu Tyr Thr Asn Asp Lys Ile Leu Ile His
        835                 840                 845             
Ile Leu Ile Arg Phe Tyr Lys Arg Ile Ile Asp Ser Ser Ile Leu Asn
    850                 855                 860                 
Met Lys Tyr Glu Asn Asn Arg Phe Ile Asp Ser Ser Gly Tyr Gly Ser
865                 870                 875                 880 
Asn Ile Ser Ile Asn Gly Asp Ile Tyr Ile Tyr Ser Thr Asn Arg Asn
                885                 890                 895     
Gln Phe Gly Ile Tyr Ser Ser Arg Leu Ser Glu Val Asn Ile Thr Gln
            900                 905                 910         
Asn Asn Thr Ile Ile Tyr Asn Ser Arg Tyr Gln Asn Phe Ser Val Ser
        915                 920                 925             
Phe Trp Val Arg Ile Pro Lys Tyr Asn Asn Leu Lys Asn Leu Asn Asn
    930                 935                 940                 
Glu Tyr Thr Ile Ile Asn Cys Met Arg Asn Asn Asn Ser Gly Trp Lys
945                 950                 955                 960 
Ile Ser Leu Asn Tyr Asn Asn Ile Ile Trp Thr Leu Gln Asp Thr Thr
                965                 970                 975     
Gly Asn Asn Gln Lys Leu Val Phe Asn Tyr Thr Gln Met Ile Asp Ile
            980                 985                 990         
Ser Asp Tyr Ile Asn Lys Trp Thr Phe Val Thr Ile Thr Asn Asn Arg
        995                 1000                1005            
Leu Gly His Ser Lys Leu Tyr Ile Asn Gly Asn Leu Thr Asp Gln Lys
    1010                1015                1020                
Ser Ile Leu Asn Leu Gly Asn Ile His Val Asp Asp Asn Ile Leu Phe
1025                1030                1035                1040
Lys Ile Val Gly Cys Asn Asp Thr Arg Tyr Val Gly Ile Arg Tyr Phe
                1045                1050                1055    
Lys Ile Phe Asn Met Glu Leu Asp Lys Thr Glu Ile Glu Thr Leu Tyr
            1060                1065                1070        
His Ser Glu Pro Asp Ser Thr Ile Leu Lys Asp Phe Trp Gly Asn Tyr
        1075                1080                1085            
Leu Leu Tyr Asn Lys Lys Tyr Tyr Leu Leu Asn Leu Leu Lys Pro Asn
    1090                1095                1100                
Met Ser Val Thr Lys Asn Ser Asp Ile Leu Asn Ile Asn Arg Gln Arg
1105                1110                1115                1120
Gly Ile Tyr Ser Lys Thr Asn Ile Phe Ser Asn Ala Arg Leu Tyr Thr
                1125                1130                1135    
Gly Val Glu Val Ile Ile Arg Lys Val Gly Ser Thr Asp Thr Ser Asn
            1140                1145                1150        
Thr Asp Asn Phe Val Arg Lys Asn Asp Thr Val Tyr Ile Asn Val Val
        1155                1160                1165            
Asp Gly Asn Ser Glu Tyr Gln Leu Tyr Ala Asp Val Ser Thr Ser Ala
    1170                1175                1180                
Val Glu Lys Thr Ile Lys Leu Arg Arg Ile Ser Asn Ser Asn Tyr Asn
1185                1190                1195                1200
Ser Asn Gln Met Ile Ile Met Asp Ser Ile Gly Asp Asn Cys Thr Met
                1205                1210                1215    
Asn Phe Lys Thr Asn Asn Gly Asn Asp Ile Gly Leu Leu Gly Phe His
            1220                1225                1230        
Leu Asn Asn Leu Val Ala Ser Ser Trp Tyr Tyr Lys Asn Ile Arg Asn
        1235                1240                1245            
Asn Thr Arg Asn Asn Gly Cys Phe Trp Ser Phe Ile Ser Lys Glu His
    1250                1255                1260                
Gly Trp Gln Glu
1265            


<210> 10
<211> 1251
<212> PRT
<213> Clostridium butyricum

<400> 10
Met Pro Thr Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asn Arg
 1               5                  10                  15      
Thr Ile Leu Tyr Ile Lys Pro Gly Gly Cys Gln Gln Phe Tyr Lys Ser
            20                  25                  30          
Phe Asn Ile Met Lys Asn Ile Trp Ile Ile Pro Glu Arg Asn Val Ile
        35                  40                  45              
Gly Thr Ile Pro Gln Asp Phe Leu Pro Pro Thr Ser Leu Lys Asn Gly
    50                  55                  60                  
Asp Ser Ser Tyr Tyr Asp Pro Asn Tyr Leu Gln Ser Asp Gln Glu Lys
65                  70                  75                  80  
Asp Lys Phe Leu Lys Ile Val Thr Lys Ile Phe Asn Arg Ile Asn Asp
                85                  90                  95      
Asn Leu Ser Gly Arg Ile Leu Leu Glu Glu Leu Ser Lys Ala Asn Pro
            100                 105                 110         
Tyr Leu Gly Asn Asp Asn Thr Pro Asp Gly Asp Phe Ile Ile Asn Asp
        115                 120                 125             
Ala Ser Ala Val Pro Ile Gln Phe Ser Asn Gly Ser Gln Ser Ile Leu
    130                 135                 140                 
Leu Pro Asn Val Ile Ile Met Gly Ala Glu Pro Asp Leu Phe Glu Thr
145                 150                 155                 160 
Asn Ser Ser Asn Ile Ser Leu Arg Asn Asn Tyr Met Pro Ser Asn His
                165                 170                 175     
Gly Phe Gly Ser Ile Ala Ile Val Thr Phe Ser Pro Glu Tyr Ser Phe
            180                 185                 190         
Arg Phe Lys Asp Asn Ser Met Asn Glu Phe Ile Gln Asp Pro Ala Leu
        195                 200                 205             
Thr Leu Met His Glu Leu Ile His Ser Leu His Gly Leu Tyr Gly Ala
    210                 215                 220                 
Lys Gly Ile Thr Thr Lys Tyr Thr Ile Thr Gln Lys Gln Asn Pro Leu
225                 230                 235                 240 
Ile Thr Asn Ile Arg Gly Thr Asn Ile Glu Glu Phe Leu Thr Phe Gly
                245                 250                 255     
Gly Thr Asp Leu Asn Ile Ile Thr Ser Ala Gln Ser Asn Asp Ile Tyr
            260                 265                 270         
Thr Asn Leu Leu Ala Asp Tyr Lys Lys Ile Ala Ser Lys Leu Ser Lys
        275                 280                 285             
Val Gln Val Ser Asn Pro Leu Leu Asn Pro Tyr Lys Asp Val Phe Glu
    290                 295                 300                 
Ala Lys Tyr Gly Leu Asp Lys Asp Ala Ser Gly Ile Tyr Ser Val Asn
305                 310                 315                 320 
Ile Asn Lys Phe Asn Asp Ile Phe Lys Lys Leu Tyr Ser Phe Thr Glu
                325                 330                 335     
Phe Asp Leu Ala Thr Lys Phe Gln Val Lys Cys Arg Gln Thr Tyr Ile
            340                 345                 350         
Gly Gln Tyr Lys Tyr Phe Lys Leu Ser Asn Leu Leu Asn Asp Ser Ile
        355                 360                 365             
Tyr Asn Ile Ser Glu Gly Tyr Asn Ile Asn Asn Leu Lys Val Asn Phe
    370                 375                 380                 
Arg Gly Gln Asn Ala Asn Leu Asn Pro Arg Ile Ile Thr Pro Ile Thr
385                 390                 395                 400 
Gly Arg Gly Leu Val Lys Lys Ile Ile Arg Phe Cys Lys Asn Ile Val
                405                 410                 415     
Ser Val Lys Gly Ile Arg Lys Ser Ile Cys Ile Glu Ile Asn Asn Gly
            420                 425                 430         
Glu Leu Phe Phe Val Ala Ser Glu Asn Ser Tyr Asn Asp Asp Asn Ile
        435                 440                 445             
Asn Thr Pro Lys Glu Ile Asp Asp Thr Val Thr Ser Asn Asn Asn Tyr
    450                 455                 460                 
Glu Asn Asp Leu Asp Gln Val Ile Leu Asn Phe Asn Ser Glu Ser Ala
465                 470                 475                 480 
Pro Gly Leu Ser Asp Glu Lys Leu Asn Leu Thr Ile Gln Asn Asp Ala
                485                 490                 495     
Tyr Ile Pro Lys Tyr Asp Ser Asn Gly Thr Ser Asp Ile Glu Gln His
            500                 505                 510         
Asp Val Asn Glu Leu Asn Val Phe Phe Tyr Leu Asp Ala Gln Lys Val
        515                 520                 525             
Pro Glu Gly Glu Asn Asn Val Asn Leu Thr Ser Ser Ile Asp Thr Ala
    530                 535                 540                 
Leu Leu Glu Gln Pro Lys Ile Tyr Thr Phe Phe Ser Ser Glu Phe Ile
545                 550                 555                 560 
Asn Asn Val Asn Lys Pro Val Gln Ala Ala Leu Phe Val Gly Trp Ile
                565                 570                 575     
Gln Gln Val Leu Val Asp Phe Thr Thr Glu Ala Asn Gln Lys Ser Thr
            580                 585                 590         
Val Asp Lys Ile Ala Asp Ile Ser Ile Val Val Pro Tyr Ile Gly Leu
        595                 600                 605             
Ala Leu Asn Ile Gly Asn Glu Ala Gln Lys Gly Asn Phe Lys Asp Ala
    610                 615                 620                 
Leu Glu Leu Leu Gly Ala Gly Ile Leu Leu Glu Phe Glu Pro Glu Leu
625                 630                 635                 640 
Leu Ile Pro Thr Ile Leu Val Phe Thr Ile Lys Ser Phe Leu Gly Ser
                645                 650                 655     
Ser Asp Asn Lys Asn Lys Val Ile Lys Ala Ile Asn Asn Ala Leu Lys
            660                 665                 670         
Glu Arg Asp Glu Lys Trp Lys Glu Val Tyr Ser Phe Ile Val Ser Asn
        675                 680                 685             
Trp Met Thr Lys Ile Asn Thr Gln Phe Asn Lys Arg Lys Glu Gln Met
    690                 695                 700                 
Tyr Gln Ala Leu Gln Asn Gln Val Asn Ala Leu Lys Ala Ile Ile Glu
705                 710                 715                 720 
Ser Lys Tyr Asn Ser Tyr Thr Leu Glu Glu Lys Asn Glu Leu Thr Asn
                725                 730                 735     
Lys Tyr Asp Ile Glu Gln Ile Glu Asn Glu Leu Asn Gln Lys Val Ser
            740                 745                 750         
Ile Ala Met Asn Asn Ile Asp Arg Phe Leu Thr Glu Ser Ser Ile Ser
        755                 760                 765             
Tyr Leu Met Lys Leu Ile Asn Glu Val Lys Ile Asn Lys Leu Arg Glu
    770                 775                 780                 
Tyr Asp Glu Asn Val Lys Thr Tyr Leu Leu Asp Tyr Ile Ile Lys His
785                 790                 795                 800 
Gly Ser Ile Leu Gly Glu Ser Gln Gln Glu Leu Asn Ser Met Val Ile
                805                 810                 815     
Asp Thr Leu Asn Asn Ser Ile Pro Phe Lys Leu Ser Ser Tyr Thr Asp
            820                 825                 830         
Asp Lys Ile Leu Ile Ser Tyr Phe Asn Lys Phe Phe Lys Arg Ile Lys
        835                 840                 845             
Ser Ser Ser Val Leu Asn Met Arg Tyr Lys Asn Asp Lys Tyr Val Asp
    850                 855                 860                 
Thr Ser Gly Tyr Asp Ser Asn Ile Asn Ile Asn Gly Asp Val Tyr Lys
865                 870                 875                 880 
Tyr Pro Thr Asn Lys Asn Gln Phe Gly Ile Tyr Asn Asp Lys Leu Ser
                885                 890                 895     
Glu Val Asn Ile Ser Gln Asn Asp Tyr Ile Ile Tyr Asp Asn Lys Tyr
            900                 905                 910         
Lys Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro Asn Tyr Asp Asn
        915                 920                 925             
Lys Ile Val Asn Val Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Arg
    930                 935                 940                 
Asp Asn Asn Ser Gly Trp Lys Val Ser Leu Asn His Asn Glu Ile Ile
945                 950                 955                 960 
Trp Thr Leu Gln Asp Asn Ser Gly Ile Asn Gln Lys Leu Ala Phe Asn
                965                 970                 975     
Tyr Gly Asn Ala Asn Gly Ile Ser Asp Tyr Ile Asn Lys Trp Ile Phe
            980                 985                 990         
Val Thr Ile Thr Asn Asp Arg Leu Gly Asp Ser Lys Leu Tyr Ile Asn
        995                 1000                1005            
Gly Asn Leu Ile Asp Lys Lys Ser Ile Leu Asn Leu Gly Asn Ile His
    1010                1015                1020                
Val Ser Asp Asn Ile Leu Phe Lys Ile Val Asn Cys Ser Tyr Thr Arg
1025                1030                1035                1040
Tyr Ile Gly Ile Arg Tyr Phe Asn Ile Phe Asp Lys Glu Leu Asp Glu
                1045                1050                1055    
Thr Glu Ile Gln Thr Leu Tyr Asn Asn Glu Pro Asn Ala Asn Ile Leu
            1060                1065                1070        
Lys Asp Phe Trp Gly Asn Tyr Leu Leu Tyr Asp Lys Glu Tyr Tyr Leu
        1075                1080                1085            
Leu Asn Val Leu Lys Pro Asn Asn Phe Ile Asn Arg Arg Thr Asp Ser
    1090                1095                1100                
Thr Leu Ser Ile Asn Asn Ile Arg Ser Thr Ile Leu Leu Ala Asn Arg
1105                1110                1115                1120
Leu Tyr Ser Gly Ile Lys Val Lys Ile Gln Arg Val Asn Asn Ser Ser
                1125                1130                1135    
Thr Asn Asp Asn Leu Val Arg Lys Asn Asp Gln Val Tyr Ile Asn Phe
            1140                1145                1150        
Val Ala Ser Lys Thr His Leu Leu Pro Leu Tyr Ala Asp Thr Ala Thr
        1155                1160                1165            
Thr Asn Lys Glu Lys Thr Ile Lys Ile Ser Ser Ser Gly Asn Arg Phe
    1170                1175                1180                
Asn Gln Val Val Val Met Asn Ser Val Gly Asn Cys Thr Met Asn Phe
1185                1190                1195                1200
Lys Asn Asn Asn Gly Asn Asn Ile Gly Leu Leu Gly Phe Lys Ala Asp
                1205                1210                1215    
Thr Val Val Ala Ser Thr Trp Tyr Tyr Thr His Met Arg Asp Asn Thr
            1220                1225                1230        
Asn Ser Asn Gly Phe Phe Trp Asn Phe Ile Ser Glu Glu His Gly Trp
        1235                1240                1245            
Gln Glu Lys
    1250    


<210> 11
<211> 1035
<212> PRT
<213> Bos taurus

<400> 11
Met Gly Ser Lys Arg Ser Val Pro Ser Arg His Arg Ser Leu Thr Thr
 1               5                  10                  15      
Tyr Glu Val Met Phe Ala Val Leu Phe Val Ile Leu Val Ala Leu Cys
            20                  25                  30          
Ala Gly Leu Ile Ala Val Ser Trp Leu Ser Ile Gln Gly Ser Val Lys
        35                  40                  45              
Asp Ala Ala Phe Gly Lys Ser His Glu Ala Arg Gly Thr Leu Lys Ile
    50                  55                  60                  
Ile Ser Gly Ala Thr Tyr Asn Pro His Leu Gln Asp Lys Leu Ser Val
65                  70                  75                  80  
Asp Phe Lys Val Leu Ala Phe Asp Ile Gln Gln Met Ile Asp Asp Ile
                85                  90                  95      
Phe Gln Ser Ser Asn Leu Lys Asn Glu Tyr Lys Asn Ser Arg Val Leu
            100                 105                 110         
Gln Phe Glu Asn Gly Ser Ile Ile Val Ile Phe Asp Leu Leu Phe Asp
        115                 120                 125             
Gln Trp Val Ser Asp Lys Asn Val Lys Glu Glu Leu Ile Gln Gly Ile
    130                 135                 140                 
Glu Ala Asn Lys Ser Ser Gln Leu Val Thr Phe His Ile Asp Leu Asn
145                 150                 155                 160 
Ser Ile Asp Ile Thr Ala Ser Leu Glu Asn Phe Ser Thr Ile Ser Pro
                165                 170                 175     
Ala Thr Thr Ser Glu Lys Leu Thr Thr Ser Ile Pro Leu Ala Thr Pro
            180                 185                 190         
Gly Asn Val Ser Ile Glu Cys Pro Pro Asp Ser Arg Leu Cys Ala Asp
        195                 200                 205             
Ala Leu Lys Cys Ile Ala Ile Asp Leu Phe Cys Asp Gly Glu Leu Asn
    210                 215                 220                 
Cys Pro Asp Gly Ser Asp Glu Asp Asn Lys Thr Cys Ala Thr Ala Cys
225                 230                 235                 240 
Asp Gly Arg Phe Leu Leu Thr Gly Ser Ser Gly Ser Phe Glu Ala Leu
                245                 250                 255     
His Tyr Pro Lys Pro Ser Asn Asn Thr Ser Ala Val Cys Arg Trp Ile
            260                 265                 270         
Ile Arg Val Asn Gln Gly Leu Ser Ile Gln Leu Asn Phe Asp Tyr Phe
        275                 280                 285             
Asn Thr Tyr Tyr Ala Asp Val Leu Asn Ile Tyr Glu Gly Met Gly Ser
    290                 295                 300                 
Ser Lys Ile Leu Arg Ala Ser Leu Trp Ser Asn Asn Pro Gly Ile Ile
305                 310                 315                 320 
Arg Ile Phe Ser Asn Gln Val Thr Ala Thr Phe Leu Ile Gln Ser Asp
                325                 330                 335     
Glu Ser Asp Tyr Ile Gly Phe Lys Val Thr Tyr Thr Ala Phe Asn Ser
            340                 345                 350         
Lys Glu Leu Asn Asn Tyr Glu Lys Ile Asn Cys Asn Phe Glu Asp Gly
        355                 360                 365             
Phe Cys Phe Trp Ile Gln Asp Leu Asn Asp Asp Asn Glu Trp Glu Arg
    370                 375                 380                 
Thr Gln Gly Ser Thr Phe Pro Pro Ser Thr Gly Pro Thr Phe Asp His
385                 390                 395                 400 
Thr Phe Gly Asn Glu Ser Gly Phe Tyr Ile Ser Thr Pro Thr Gly Pro
                405                 410                 415     
Gly Gly Arg Arg Glu Arg Val Gly Leu Leu Thr Leu Pro Leu Asp Pro
            420                 425                 430         
Thr Pro Glu Gln Ala Cys Leu Ser Phe Trp Tyr Tyr Met Tyr Gly Glu
        435                 440                 445             
Asn Val Tyr Lys Leu Ser Ile Asn Ile Ser Ser Asp Gln Asn Met Glu
    450                 455                 460                 
Lys Thr Ile Phe Gln Lys Glu Gly Asn Tyr Gly Gln Asn Trp Asn Tyr
465                 470                 475                 480 
Gly Gln Val Thr Leu Asn Glu Thr Val Glu Phe Lys Val Ser Phe Tyr
                485                 490                 495     
Gly Phe Lys Asn Gln Ile Leu Ser Asp Ile Ala Leu Asp Asp Ile Ser
            500                 505                 510         
Leu Thr Tyr Gly Ile Cys Asn Val Ser Val Tyr Pro Glu Pro Thr Leu
        515                 520                 525             
Val Pro Thr Pro Pro Pro Glu Leu Pro Thr Asp Cys Gly Gly Pro His
    530                 535                 540                 
Asp Leu Trp Glu Pro Asn Thr Thr Phe Thr Ser Ile Asn Phe Pro Asn
545                 550                 555                 560 
Ser Tyr Pro Asn Gln Ala Phe Cys Ile Trp Asn Leu Asn Ala Gln Lys
                565                 570                 575     
Gly Lys Asn Ile Gln Leu His Phe Gln Glu Phe Asp Leu Glu Asn Ile
            580                 585                 590         
Ala Asp Val Val Glu Ile Arg Asp Gly Glu Gly Asp Asp Ser Leu Phe
        595                 600                 605             
Leu Ala Val Tyr Thr Gly Pro Gly Pro Val Asn Asp Val Phe Ser Thr
    610                 615                 620                 
Thr Asn Arg Met Thr Val Leu Phe Ile Thr Asp Asn Met Leu Ala Lys
625                 630                 635                 640 
Gln Gly Phe Lys Ala Asn Phe Thr Thr Gly Tyr Gly Leu Gly Ile Pro
                645                 650                 655     
Glu Pro Cys Lys Glu Asp Asn Phe Gln Cys Lys Asp Gly Glu Cys Ile
            660                 665                 670         
Pro Leu Val Asn Leu Cys Asp Gly Phe Pro His Cys Lys Asp Gly Ser
        675                 680                 685             
Asp Glu Ala His Cys Val Arg Leu Phe Asn Gly Thr Thr Asp Ser Ser
    690                 695                 700                 
Gly Leu Val Gln Phe Arg Ile Gln Ser Ile Trp His Val Ala Cys Ala
705                 710                 715                 720 
Glu Asn Trp Thr Thr Gln Ile Ser Asp Asp Val Cys Gln Leu Leu Gly
                725                 730                 735     
Leu Gly Thr Gly Asn Ser Ser Val Pro Thr Phe Ser Thr Gly Gly Gly
            740                 745                 750         
Pro Tyr Val Asn Leu Asn Thr Ala Pro Asn Gly Ser Leu Ile Leu Thr
        755                 760                 765             
Pro Ser Gln Gln Cys Leu Glu Asp Ser Leu Ile Leu Leu Gln Cys Asn
    770                 775                 780                 
Tyr Lys Ser Cys Gly Lys Lys Leu Val Thr Gln Glu Val Ser Pro Lys
785                 790                 795                 800 
Ile Val Gly Gly Ser Asp Ser Arg Glu Gly Ala Trp Pro Trp Val Val
                805                 810                 815     
Ala Leu Tyr Phe Asp Asp Gln Gln Val Cys Gly Ala Ser Leu Val Ser
            820                 825                 830         
Arg Asp Trp Leu Val Ser Ala Ala His Cys Val Tyr Gly Arg Asn Met
        835                 840                 845             
Glu Pro Ser Lys Trp Lys Ala Val Leu Gly Leu His Met Ala Ser Asn
    850                 855                 860                 
Leu Thr Ser Pro Gln Ile Glu Thr Arg Leu Ile Asp Gln Ile Val Ile
865                 870                 875                 880 
Asn Pro His Tyr Asn Lys Arg Arg Lys Asn Asn Asp Ile Ala Met Met
                885                 890                 895     
His Leu Glu Met Lys Val Asn Tyr Thr Asp Tyr Ile Gln Pro Ile Cys
            900                 905                 910         
Leu Pro Glu Glu Asn Gln Val Phe Pro Pro Gly Arg Ile Cys Ser Ile
        915                 920                 925             
Ala Gly Trp Gly Ala Leu Ile Tyr Gln Gly Ser Thr Ala Asp Val Leu
    930                 935                 940                 
Gln Glu Ala Asp Val Pro Leu Leu Ser Asn Glu Lys Cys Gln Gln Gln
945                 950                 955                 960 
Met Pro Glu Tyr Asn Ile Thr Glu Asn Met Val Cys Ala Gly Tyr Glu
                965                 970                 975     
Ala Gly Gly Val Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro Leu Met
            980                 985                 990         
Cys Gln Glu Asn Asn Arg Trp Leu Leu Ala Gly Val Thr Ser Phe Gly
        995                 1000                1005            
Tyr Gln Cys Ala Leu Pro Asn Arg Pro Gly Val Tyr Ala Arg Val Pro
    1010                1015                1020                
Arg Phe Thr Glu Trp Ile Gln Ser Phe Leu His
1025                1030                1035


<210> 12
<211> 183
<212> PRT
<213> Human rhinovirus C

<400> 12
Gly Pro Glu His Glu Phe Leu Asn Ala Leu Ile Arg Arg Asn Cys His
 1               5                  10                  15      
Ile Ile Thr Thr Asp Lys Gly Glu Phe Asn Leu Leu Gly Ile Tyr Ser
            20                  25                  30          
Asn Cys Ala Val Val Pro Thr His Ala Glu Pro Gly Asp Val Val Asp
        35                  40                  45              
Ile Asp Gly Arg Leu Val Arg Val Leu Lys Gln Gln Val Leu Thr Asp
    50                  55                  60                  
Met Asn Asp Val Asp Thr Glu Val Thr Val Leu Trp Leu Asp Gln Asn
65                  70                  75                  80  
Glu Lys Phe Arg Asp Ile Arg Arg Phe Ile Pro Glu His Gln Gln Asp
                85                  90                  95      
Trp His Asn Ile His Leu Ala Thr Asn Val Thr Lys Phe Pro Met Leu
            100                 105                 110         
Asn Val Glu Val Gly His Thr Val Pro Tyr Gly Glu Ile Asn Leu Ser
        115                 120                 125             
Gly Asn Ala Thr Cys Arg Leu Tyr Lys Tyr Asp Tyr Pro Thr Gln Pro
    130                 135                 140                 
Gly Gln Cys Gly Ala Val Leu Ala Asn Thr Gly Asn Ile Ile Gly Ile
145                 150                 155                 160 
His Val Gly Gly Asn Gly Arg Val Gly Tyr Ala Ala Ala Leu Leu Arg
                165                 170                 175     
Lys Tyr Phe Ala Glu Glu Gln
            180             


<210> 13
<211> 183
<212> PRT
<213> Human enterovirus 71

<400> 13
Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Val Arg
 1               5                  10                  15      
Gln Val Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp
            20                  25                  30          
Arg Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp
        35                  40                  45              
Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp
    50                  55                  60                  
Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Ile Thr Leu Asp Thr Asn
65                  70                  75                  80  
Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Asn Ile Ser Thr
                85                  90                  95      
Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met
            100                 105                 110         
Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Xaa Leu Asn Leu Ser
        115                 120                 125             
Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala
    130                 135                 140                 
Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Val Ile Gly Ile
145                 150                 155                 160 
His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg
                165                 170                 175     
Ser Tyr Phe Ala Ser Glu Gln
            180             


<210> 14
<211> 3054
<212> PRT
<213> Potyvirus

<400> 14
Met Ala Leu Ile Phe Gly Thr Val Asn Ala Asn Ile Leu Lys Glu Val
 1               5                  10                  15      
Phe Gly Gly Ala Arg Met Ala Cys Val Thr Ser Ala His Met Ala Gly
            20                  25                  30          
Ala Asn Gly Ser Ile Leu Lys Lys Ala Glu Glu Thr Ser Arg Ala Ile
        35                  40                  45              
Met His Lys Pro Val Ile Phe Gly Glu Asp Tyr Ile Thr Glu Ala Asp
    50                  55                  60                  
Leu Pro Tyr Thr Pro Leu His Leu Glu Val Asp Ala Glu Met Glu Arg
65                  70                  75                  80  
Met Tyr Tyr Leu Gly Arg Arg Ala Leu Thr His Gly Lys Arg Arg Lys
                85                  90                  95      
Val Ser Val Asn Asn Lys Arg Asn Arg Arg Arg Lys Val Ala Lys Thr
            100                 105                 110         
Tyr Val Gly Arg Asp Ser Ile Val Glu Lys Ile Val Val Pro His Thr
        115                 120                 125             
Glu Arg Lys Val Asp Thr Thr Ala Ala Val Glu Asp Ile Cys Asn Glu
    130                 135                 140                 
Ala Thr Thr Gln Leu Val His Asn Ser Met Pro Lys Arg Lys Lys Gln
145                 150                 155                 160 
Lys Asn Phe Leu Pro Ala Thr Ser Leu Ser Asn Val Tyr Ala Gln Thr
                165                 170                 175     
Trp Ser Ile Val Arg Lys Arg His Met Gln Val Glu Ile Ile Ser Lys
            180                 185                 190         
Lys Ser Val Arg Ala Arg Val Lys Arg Phe Glu Gly Ser Val Gln Leu
        195                 200                 205             
Phe Ala Ser Val Arg His Met Tyr Gly Glu Arg Lys Arg Val Asp Leu
    210                 215                 220                 
Arg Ile Asp Asn Trp Gln Gln Glu Thr Leu Leu Asp Leu Ala Lys Arg
225                 230                 235                 240 
Phe Lys Asn Glu Arg Val Asp Gln Ser Lys Leu Thr Phe Gly Ser Ser
                245                 250                 255     
Gly Leu Val Leu Arg Gln Gly Ser Tyr Gly Pro Ala His Trp Tyr Arg
            260                 265                 270         
His Gly Met Phe Ile Val Arg Gly Arg Ser Asp Gly Met Leu Val Asp
        275                 280                 285             
Ala Arg Ala Lys Val Thr Phe Ala Val Cys His Ser Met Thr His Tyr
    290                 295                 300                 
Ser Asp Lys Ser Ile Ser Glu Ala Phe Phe Ile Pro Tyr Ser Lys Lys
305                 310                 315                 320 
Phe Leu Glu Leu Arg Pro Asp Gly Ile Ser His Glu Cys Thr Arg Gly
                325                 330                 335     
Val Ser Val Glu Arg Cys Gly Glu Val Ala Ala Ile Leu Thr Gln Ala
            340                 345                 350         
Leu Ser Pro Cys Gly Lys Ile Thr Cys Lys Arg Cys Met Val Glu Thr
        355                 360                 365             
Pro Asp Ile Val Glu Gly Glu Ser Gly Glu Ser Val Thr Asn Gln Gly
    370                 375                 380                 
Lys Leu Leu Ala Met Leu Lys Glu Gln Tyr Pro Asp Phe Pro Met Ala
385                 390                 395                 400 
Glu Lys Leu Leu Thr Arg Phe Leu Gln Gln Lys Ser Leu Val Asn Thr
                405                 410                 415     
Asn Leu Thr Ala Cys Val Ser Val Lys Gln Leu Ile Gly Asp Arg Lys
            420                 425                 430         
Gln Ala Pro Phe Thr His Val Leu Ala Val Ser Glu Ile Leu Phe Lys
        435                 440                 445             
Gly Asn Lys Leu Thr Gly Ala Asp Leu Glu Glu Ala Ser Thr His Met
    450                 455                 460                 
Leu Glu Ile Ala Arg Phe Leu Asn Asn Arg Thr Glu Asn Met Arg Ile
465                 470                 475                 480 
Gly His Leu Gly Ser Phe Arg Asn Lys Ile Ser Ser Lys Ala His Val
                485                 490                 495     
Asn Asn Ala Leu Met Cys Asp Asn Gln Leu Asp Gln Asn Gly Asn Phe
            500                 505                 510         
Ile Trp Gly Leu Arg Gly Ala His Ala Lys Arg Phe Leu Lys Gly Phe
        515                 520                 525             
Phe Thr Glu Ile Asp Pro Asn Glu Gly Tyr Asp Lys Tyr Val Ile Arg
    530                 535                 540                 
Lys His Ile Arg Gly Ser Arg Lys Leu Ala Ile Gly Asn Leu Ile Met
545                 550                 555                 560 
Ser Thr Asp Phe Gln Thr Leu Arg Gln Gln Ile Gln Gly Glu Thr Ile
                565                 570                 575     
Glu Arg Lys Glu Ile Gly Asn His Cys Ile Ser Met Arg Asn Gly Asn
            580                 585                 590         
Tyr Val Tyr Pro Cys Cys Cys Val Thr Leu Glu Asp Gly Lys Ala Gln
        595                 600                 605             
Tyr Ser Asp Leu Lys His Pro Thr Lys Arg His Leu Val Ile Gly Asn
    610                 615                 620                 
Ser Gly Asp Ser Lys Tyr Leu Asp Leu Pro Val Leu Asn Glu Glu Lys
625                 630                 635                 640 
Met Tyr Ile Ala Asn Glu Gly Tyr Cys Tyr Met Asn Ile Phe Phe Ala
                645                 650                 655     
Leu Leu Val Asn Val Lys Glu Glu Asp Ala Lys Asp Phe Thr Lys Phe
            660                 665                 670         
Ile Arg Asp Thr Ile Val Pro Lys Leu Gly Ala Trp Pro Thr Met Gln
        675                 680                 685             
Asp Val Ala Thr Ala Cys Tyr Leu Leu Ser Ile Leu Tyr Pro Asp Val
    690                 695                 700                 
Leu Arg Ala Glu Leu Pro Arg Ile Leu Val Asp His Asp Asn Lys Thr
705                 710                 715                 720 
Met His Val Leu Asp Ser Tyr Gly Ser Arg Thr Thr Gly Tyr His Met
                725                 730                 735     
Leu Lys Met Asn Thr Thr Ser Gln Leu Ile Glu Phe Val His Ser Gly
            740                 745                 750         
Leu Glu Ser Glu Met Lys Thr Tyr Asn Val Gly Gly Met Asn Arg Asp
        755                 760                 765             
Val Val Thr Gln Gly Ala Ile Glu Met Leu Ile Lys Ser Ile Tyr Lys
    770                 775                 780                 
Pro His Leu Met Lys Gln Leu Leu Glu Glu Glu Pro Tyr Ile Ile Val
785                 790                 795                 800 
Leu Ala Ile Val Ser Pro Ser Ile Leu Ile Ala Met Tyr Asn Ser Gly
                805                 810                 815     
Thr Phe Glu Gln Ala Leu Gln Met Trp Leu Pro Asn Thr Met Arg Leu
            820                 825                 830         
Ala Asn Leu Ala Ala Ile Leu Ser Ala Leu Ala Gln Lys Leu Thr Leu
        835                 840                 845             
Ala Asp Leu Phe Val Gln Gln Arg Asn Leu Ile Asn Glu Tyr Ala Gln
    850                 855                 860                 
Val Ile Leu Asp Asn Leu Ile Asp Gly Val Arg Val Asn His Ser Leu
865                 870                 875                 880 
Ser Leu Ala Met Glu Ile Val Thr Ile Lys Leu Ala Thr Gln Glu Met
                885                 890                 895     
Asp Met Ala Leu Arg Glu Gly Gly Tyr Ala Val Thr Ser Glu Lys Val
            900                 905                 910         
His Glu Met Leu Glu Lys Asn Tyr Val Lys Ala Leu Lys Asp Ala Trp
        915                 920                 925             
Asp Glu Leu Thr Trp Leu Glu Lys Phe Ser Ala Ile Arg His Ser Arg
    930                 935                 940                 
Lys Leu Leu Lys Phe Gly Arg Lys Pro Leu Ile Met Lys Asn Thr Val
945                 950                 955                 960 
Asp Cys Gly Gly His Ile Asp Leu Ser Val Lys Ser Leu Phe Lys Phe
                965                 970                 975     
His Leu Glu Leu Leu Lys Gly Thr Ile Ser Arg Ala Val Asn Gly Gly
            980                 985                 990         
Ala Arg Lys Val Arg Val Ala Lys Asn Ala Met Thr Lys Gly Val Phe
        995                 1000                1005            
Leu Lys Ile Tyr Ser Met Leu Pro Asp Val Tyr Lys Phe Ile Thr Val
    1010                1015                1020                
Ser Ser Val Leu Ser Leu Leu Leu Thr Phe Leu Phe Gln Ile Asp Cys
1025                1030                1035                1040
Met Ile Arg Ala His Arg Glu Ala Lys Val Ala Ala Gln Leu Gln Lys
                1045                1050                1055    
Glu Ser Glu Trp Asp Asn Ile Ile Asn Arg Thr Phe Gln Tyr Ser Lys
            1060                1065                1070        
Leu Glu Asn Pro Ile Gly Tyr Arg Ser Thr Ala Glu Glu Arg Leu Gln
        1075                1080                1085            
Ser Glu His Pro Glu Ala Phe Glu Tyr Tyr Lys Phe Cys Ile Gly Lys
    1090                1095                1100                
Glu Asp Leu Val Glu Gln Ala Lys Gln Pro Glu Ile Ala Tyr Phe Glu
1105                1110                1115                1120
Lys Ile Ile Ala Phe Ile Thr Leu Val Leu Met Ala Phe Asp Ala Glu
                1125                1130                1135    
Arg Ser Asp Gly Val Phe Lys Ile Leu Asn Lys Phe Lys Gly Ile Leu
            1140                1145                1150        
Ser Ser Thr Glu Arg Glu Ile Ile Tyr Thr Gln Ser Leu Asp Asp Tyr
        1155                1160                1165            
Val Thr Thr Phe Asp Asp Asn Met Thr Ile Asn Leu Glu Leu Asn Met
    1170                1175                1180                
Asp Glu Leu His Lys Thr Ser Leu Pro Gly Val Thr Phe Lys Gln Trp
1185                1190                1195                1200
Trp Asn Asn Gln Ile Ser Arg Gly Asn Val Lys Pro His Tyr Arg Thr
                1205                1210                1215    
Glu Gly His Phe Met Glu Phe Thr Arg Asp Thr Ala Ala Ser Val Ala
            1220                1225                1230        
Ser Glu Ile Ser His Ser Pro Ala Arg Asp Phe Leu Val Arg Gly Ala
        1235                1240                1245            
Val Gly Ser Gly Lys Ser Thr Gly Leu Pro Tyr His Leu Ser Lys Arg
    1250                1255                1260                
Gly Arg Val Leu Met Leu Glu Pro Thr Arg Pro Leu Thr Asp Asn Met
1265                1270                1275                1280
His Lys Gln Leu Arg Ser Glu Pro Phe Asn Cys Phe Pro Thr Leu Arg
                1285                1290                1295    
Met Arg Gly Lys Ser Thr Phe Gly Ser Ser Pro Ile Thr Val Met Thr
            1300                1305                1310        
Ser Gly Phe Ala Leu His His Phe Ala Arg Asn Ile Ala Glu Val Lys
        1315                1320                1325            
Thr Tyr Asp Phe Val Ile Ile Asp Glu Cys His Val Asn Asp Ala Ser
    1330                1335                1340                
Ala Ile Ala Phe Arg Asn Leu Leu Phe Glu His Glu Phe Glu Gly Lys
1345                1350                1355                1360
Val Leu Lys Val Ser Ala Thr Pro Pro Gly Arg Glu Val Glu Phe Thr
                1365                1370                1375    
Thr Gln Phe Pro Val Lys Leu Lys Ile Glu Glu Ala Leu Ser Phe Gln
            1380                1385                1390        
Glu Phe Val Ser Leu Gln Gly Thr Gly Ala Asn Ala Asp Val Ile Ser
        1395                1400                1405            
Cys Gly Asp Asn Ile Leu Val Tyr Val Ala Ser Tyr Asn Asp Val Asp
    1410                1415                1420                
Ser Leu Gly Lys Leu Leu Val Gln Lys Gly Tyr Lys Val Ser Lys Ile
1425                1430                1435                1440
Asp Gly Arg Thr Met Lys Ser Gly Gly Thr Glu Ile Ile Thr Glu Gly
                1445                1450                1455    
Thr Ser Val Lys Lys His Phe Ile Val Ala Thr Asn Ile Ile Glu Asn
            1460                1465                1470        
Gly Val Thr Ile Asp Ile Asp Val Val Val Asp Phe Gly Thr Lys Val
        1475                1480                1485            
Val Pro Val Leu Asp Val Asp Asn Arg Ala Val Gln Tyr Asn Lys Thr
    1490                1495                1500                
Val Val Ser Tyr Gly Glu Arg Ile Gln Lys Leu Gly Arg Val Gly Arg
1505                1510                1515                1520
His Lys Glu Gly Val Ala Leu Arg Ile Gly Gln Thr Asn Lys Thr Leu
                1525                1530                1535    
Val Glu Ile Pro Glu Met Val Ala Thr Glu Ala Ala Phe Leu Cys Phe
            1540                1545                1550        
Met Tyr Asn Leu Pro Val Thr Thr Gln Ser Val Ser Thr Thr Leu Leu
        1555                1560                1565            
Glu Asn Ala Thr Leu Leu Gln Ala Arg Thr Met Ala Gln Phe Glu Leu
    1570                1575                1580                
Ser Tyr Phe Tyr Thr Ile Asn Phe Val Arg Phe Asp Gly Ser Met His
1585                1590                1595                1600
Pro Val Ile His Asp Lys Leu Lys Arg Phe Lys Leu His Thr Cys Glu
                1605                1610                1615    
Thr Phe Leu Asn Lys Leu Ala Ile Pro Asn Lys Gly Leu Ser Ser Trp
            1620                1625                1630        
Leu Thr Ser Gly Glu Tyr Lys Arg Leu Gly Tyr Ile Ala Glu Asp Ala
        1635                1640                1645            
Gly Ile Arg Ile Pro Phe Val Cys Lys Glu Ile Pro Asp Ser Leu His
    1650                1655                1660                
Glu Glu Ile Trp His Ile Val Val Ala His Lys Gly Asp Ser Gly Ile
1665                1670                1675                1680
Gly Arg Leu Thr Ser Val Gln Ala Ala Lys Val Val Tyr Thr Leu Gln
                1685                1690                1695    
Thr Asp Val His Ser Ile Ala Arg Thr Leu Ala Cys Ile Asn Arg Arg
            1700                1705                1710        
Ile Ala Asp Glu Gln Met Lys Gln Ser His Phe Glu Ala Ala Thr Gly
        1715                1720                1725            
Arg Ala Phe Ser Phe Thr Asn Tyr Ser Ile Gln Ser Ile Phe Asp Thr
    1730                1735                1740                
Leu Lys Ala Asn Tyr Ala Thr Lys His Thr Lys Glu Asn Ile Ala Val
1745                1750                1755                1760
Leu Gln Gln Ala Lys Asp Gln Leu Leu Glu Phe Ser Asn Leu Ala Lys
                1765                1770                1775    
Asp Gln Asp Val Thr Gly Ile Ile Gln Asp Phe Asn His Leu Glu Thr
            1780                1785                1790        
Ile Tyr Leu Gln Ser Asp Ser Glu Val Ala Lys His Leu Lys Leu Lys
        1795                1800                1805            
Ser His Trp Asn Lys Ser Gln Ile Thr Arg Asp Ile Ile Ile Ala Leu
    1810                1815                1820                
Ser Val Leu Ile Gly Gly Gly Trp Met Leu Ala Thr Tyr Phe Lys Asp
1825                1830                1835                1840
Lys Phe Asn Glu Pro Val Tyr Phe Gln Gly Lys Lys Asn Gln Lys His
                1845                1850                1855    
Lys Leu Lys Met Arg Glu Ala Arg Gly Ala Arg Gly Gln Tyr Glu Val
            1860                1865                1870        
Ala Ala Glu Pro Glu Ala Leu Glu His Tyr Phe Gly Ser Ala Tyr Asn
        1875                1880                1885            
Asn Lys Gly Lys Arg Lys Gly Thr Thr Arg Gly Met Gly Ala Lys Ser
    1890                1895                1900                
Arg Lys Phe Ile Asn Met Tyr Gly Phe Asp Pro Thr Asp Phe Ser Tyr
1905                1910                1915                1920
Ile Arg Phe Val Asp Pro Leu Thr Gly His Thr Ile Asp Glu Ser Thr
                1925                1930                1935    
Asn Ala Pro Ile Asp Leu Val Gln His Glu Phe Gly Lys Val Arg Thr
            1940                1945                1950        
Arg Met Leu Ile Asp Asp Glu Ile Glu Pro Gln Ser Leu Ser Thr His
        1955                1960                1965            
Thr Thr Ile His Ala Tyr Leu Val Asn Ser Gly Thr Lys Lys Val Leu
    1970                1975                1980                
Lys Val Asp Leu Thr Pro His Ser Ser Leu Arg Ala Ser Glu Lys Ser
1985                1990                1995                2000
Thr Ala Ile Met Gly Phe Pro Glu Arg Glu Asn Glu Leu Arg Gln Thr
                2005                2010                2015    
Gly Met Ala Val Pro Val Ala Tyr Asp Gln Leu Pro Pro Lys Asn Glu
            2020                2025                2030        
Asp Leu Thr Phe Glu Gly Glu Ser Leu Phe Lys Gly Pro Arg Asp Tyr
        2035                2040                2045            
Asn Pro Ile Ser Ser Thr Ile Cys His Leu Thr Asn Glu Ser Asp Gly
    2050                2055                2060                
His Thr Thr Ser Leu Tyr Gly Ile Gly Phe Gly Pro Phe Ile Ile Thr
2065                2070                2075                2080
Asn Lys His Leu Phe Arg Arg Asn Asn Gly Thr Leu Leu Val Gln Ser
                2085                2090                2095    
Leu His Gly Val Phe Lys Val Lys Asn Thr Thr Thr Leu Gln Gln His
            2100                2105                2110        
Leu Ile Asp Gly Arg Asp Met Ile Ile Ile Arg Met Pro Lys Asp Phe
        2115                2120                2125            
Pro Pro Phe Pro Gln Lys Leu Lys Phe Arg Glu Pro Gln Arg Glu Glu
    2130                2135                2140                
Arg Ile Cys Leu Val Thr Thr Asn Phe Gln Thr Lys Ser Met Ser Ser
2145                2150                2155                2160
Met Val Ser Asp Thr Ser Cys Thr Phe Pro Ser Ser Asp Gly Ile Phe
                2165                2170                2175    
Trp Lys His Trp Ile Gln Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu
            2180                2185                2190        
Val Ser Thr Arg Asp Gly Phe Ile Val Gly Ile His Ser Ala Ser Asn
        2195                2200                2205            
Phe Thr Asn Thr Asn Asn Tyr Phe Thr Ser Val Pro Lys Asn Phe Met
    2210                2215                2220                
Glu Leu Leu Thr Asn Gln Glu Ala Gln Gln Trp Val Ser Gly Trp Arg
2225                2230                2235                2240
Leu Asn Ala Asp Ser Val Leu Trp Gly Gly His Lys Val Phe Met Ser
                2245                2250                2255    
Lys Pro Glu Glu Pro Phe Gln Pro Val Lys Glu Ala Thr Gln Leu Met
            2260                2265                2270        
Asn Glu Leu Val Tyr Ser Gln Gly Glu Lys Arg Lys Trp Val Val Glu
        2275                2280                2285            
Ala Leu Ser Gly Asn Leu Arg Pro Val Ala Glu Cys Pro Ser Gln Leu
    2290                2295                2300                
Val Thr Lys His Val Val Lys Gly Lys Cys Pro Leu Phe Glu Leu Tyr
2305                2310                2315                2320
Leu Gln Leu Asn Pro Glu Lys Glu Ala Tyr Phe Lys Pro Met Met Gly
                2325                2330                2335    
Ala Tyr Lys Pro Ser Arg Leu Asn Arg Glu Ala Phe Leu Lys Asp Ile
            2340                2345                2350        
Leu Lys Tyr Ala Ser Glu Ile Glu Ile Gly Asn Val Asp Cys Asp Leu
        2355                2360                2365            
Leu Glu Leu Ala Ile Ser Met Leu Val Thr Lys Leu Lys Ala Leu Gly
    2370                2375                2380                
Phe Pro Thr Val Asn Tyr Ile Thr Asp Pro Glu Glu Ile Phe Ser Ala
2385                2390                2395                2400
Leu Asn Met Lys Ala Ala Met Gly Ala Leu Tyr Lys Gly Lys Lys Lys
                2405                2410                2415    
Glu Ala Leu Ser Glu Leu Thr Leu Asp Glu Gln Glu Ala Met Leu Lys
            2420                2425                2430        
Ala Ser Cys Leu Arg Leu Tyr Thr Gly Lys Leu Gly Ile Trp Asn Gly
        2435                2440                2445            
Ser Leu Lys Ala Glu Leu Arg Pro Ile Glu Lys Val Glu Asn Asn Lys
    2450                2455                2460                
Thr Arg Thr Phe Thr Ala Ala Pro Ile Asp Thr Leu Leu Ala Gly Lys
2465                2470                2475                2480
Val Cys Val Asp Asp Phe Asn Asn Gln Phe Tyr Asp Leu Asn Ile Lys
                2485                2490                2495    
Ala Pro Trp Thr Val Gly Met Thr Lys Phe Tyr Gln Gly Trp Asn Glu
            2500                2505                2510        
Leu Met Glu Ala Leu Pro Ser Gly Trp Val Tyr Cys Asp Ala Asp Gly
        2515                2520                2525            
Ser Gln Phe Asp Ser Ser Leu Thr Pro Phe Leu Ile Asn Ala Val Leu
    2530                2535                2540                
Lys Val Arg Leu Ala Phe Met Glu Glu Trp Asp Ile Gly Glu Gln Met
2545                2550                2555                2560
Leu Arg Asn Leu Tyr Thr Glu Ile Val Tyr Thr Pro Ile Leu Thr Pro
                2565                2570                2575    
Asp Gly Thr Ile Ile Lys Lys His Lys Gly Asn Asn Ser Gly Gln Pro
            2580                2585                2590        
Ser Thr Val Val Asp Asn Thr Leu Met Val Ile Ile Ala Met Leu Tyr
        2595                2600                2605            
Thr Cys Glu Lys Cys Gly Ile Asn Lys Glu Glu Ile Val Tyr Tyr Val
    2610                2615                2620                
Asn Gly Asp Asp Leu Leu Ile Ala Ile His Pro Asp Lys Ala Glu Arg
2625                2630                2635                2640
Leu Ser Arg Phe Lys Glu Ser Phe Gly Glu Leu Gly Leu Lys Tyr Glu
                2645                2650                2655    
Phe Asp Cys Thr Thr Arg Asp Lys Thr Gln Leu Trp Phe Met Ser His
            2660                2665                2670        
Arg Ala Leu Glu Arg Asp Gly Met Tyr Ile Pro Lys Leu Glu Glu Glu
        2675                2680                2685            
Arg Ile Val Ser Ile Leu Glu Trp Asp Arg Ser Lys Glu Pro Ser His
    2690                2695                2700                
Arg Leu Glu Ala Ile Cys Ala Ser Met Ile Glu Ala Trp Gly Tyr Asp
2705                2710                2715                2720
Lys Leu Val Glu Glu Ile Arg Asn Phe Tyr Ala Trp Val Leu Glu Gln
                2725                2730                2735    
Ala Pro Tyr Ser Gln Leu Ala Glu Glu Gly Lys Ala Pro Tyr Leu Ala
            2740                2745                2750        
Glu Thr Ala Leu Lys Phe Leu Tyr Thr Ser Gln His Gly Thr Asn Ser
        2755                2760                2765            
Glu Ile Glu Glu Tyr Leu Lys Val Leu Tyr Asp Tyr Asp Ile Pro Thr
    2770                2775                2780                
Thr Glu Asn Leu Tyr Phe Gln Ser Gly Thr Val Asp Ala Gly Ala Asp
2785                2790                2795                2800
Ala Gly Lys Lys Lys Asp Gln Lys Asp Asp Lys Val Ala Glu Gln Ala
                2805                2810                2815    
Ser Lys Asp Arg Asp Val Asn Ala Gly Thr Ser Gly Thr Phe Ser Val
            2820                2825                2830        
Pro Arg Ile Asn Ala Met Ala Thr Lys Leu Gln Tyr Pro Arg Met Arg
        2835                2840                2845            
Gly Glu Val Val Val Asn Leu Asn His Leu Leu Gly Tyr Lys Pro Gln
    2850                2855                2860                
Gln Ile Asp Leu Ser Asn Ala Arg Ala Thr His Glu Gln Phe Ala Ala
2865                2870                2875                2880
Trp His Gln Ala Val Met Thr Ala Tyr Gly Val Asn Glu Glu Gln Met
                2885                2890                2895    
Lys Ile Leu Leu Asn Gly Phe Met Val Trp Cys Ile Glu Asn Gly Thr
            2900                2905                2910        
Ser Pro Asn Leu Asn Gly Thr Trp Val Met Met Asp Gly Glu Asp Gln
        2915                2920                2925            
Val Ser Tyr Pro Leu Lys Pro Met Val Glu Asn Ala Gln Pro Thr Leu
    2930                2935                2940                
Arg Gln Ile Met Thr His Phe Ser Asp Leu Ala Glu Ala Tyr Ile Glu
2945                2950                2955                2960
Met Arg Asn Arg Glu Arg Pro Tyr Met Pro Arg Tyr Gly Leu Gln Arg
                2965                2970                2975    
Asn Ile Thr Asp Met Ser Leu Ser Arg Tyr Ala Phe Asp Phe Tyr Glu
            2980                2985                2990        
Leu Thr Ser Lys Thr Pro Val Arg Ala Arg Glu Ala His Met Gln Met
        2995                3000                3005            
Lys Ala Ala Ala Val Arg Asn Ser Gly Thr Arg Leu Phe Gly Leu Asp
    3010                3015                3020                
Gly Asn Val Gly Thr Ala Glu Glu Asp Thr Glu Arg His Thr Ala His
3025                3030                3035                3040
Asp Val Asn Arg Asn Met His Thr Leu Leu Gly Val Arg Gln
                3045                3050                


<210> 15
<211> 242
<212> PRT
<213> Potyvirus

<400> 15
Gly Glu Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Thr Ile Cys His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser Leu
            20                  25                  30          
Tyr Gly Ile Gly Phe Gly Pro Phe Ile Ile Thr Asn Lys His Leu Phe
        35                  40                  45              
Arg Arg Asn Asn Gly Thr Leu Leu Val Gln Ser Leu His Gly Val Phe
    50                  55                  60                  
Lys Val Lys Asn Thr Thr Thr Leu Gln Gln His Leu Ile Asp Gly Arg
65                  70                  75                  80  
Asp Met Ile Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Lys Leu Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu Val
            100                 105                 110         
Thr Thr Asn Phe Gln Thr Lys Ser Met Ser Ser Met Val Ser Asp Thr
        115                 120                 125             
Ser Cys Thr Phe Pro Ser Ser Asp Gly Ile Phe Trp Lys His Trp Ile
    130                 135                 140                 
Gln Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg Asp
145                 150                 155                 160 
Gly Phe Ile Val Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr Asn
                165                 170                 175     
Asn Tyr Phe Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr Asn
            180                 185                 190         
Gln Glu Ala Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp Ser
        195                 200                 205             
Val Leu Trp Gly Gly His Lys Val Phe Met Ser Lys Pro Glu Glu Pro
    210                 215                 220                 
Phe Gln Pro Val Lys Glu Ala Thr Gln Leu Met Asn Glu Leu Val Tyr
225                 230                 235                 240 
Ser Gln
        


<210> 16
<211> 242
<212> PRT
<213> Potyvirus

<220> 
<221> VARIANT        
<222> 216
<223> S219N

<400> 16
Gly Glu Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Thr Ile Cys His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser Leu
            20                  25                  30          
Tyr Gly Ile Gly Phe Gly Pro Phe Ile Ile Thr Asn Lys His Leu Phe
        35                  40                  45              
Arg Arg Asn Asn Gly Thr Leu Leu Val Gln Ser Leu His Gly Val Phe
    50                  55                  60                  
Lys Val Lys Asn Thr Thr Thr Leu Gln Gln His Leu Ile Asp Gly Arg
65                  70                  75                  80  
Asp Met Ile Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Lys Leu Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu Val
            100                 105                 110         
Thr Thr Asn Phe Gln Thr Lys Ser Met Ser Ser Met Val Ser Asp Thr
        115                 120                 125             
Ser Cys Thr Phe Pro Ser Ser Asp Gly Ile Phe Trp Lys His Trp Ile
    130                 135                 140                 
Gln Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg Asp
145                 150                 155                 160 
Gly Phe Ile Val Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr Asn
                165                 170                 175     
Asn Tyr Phe Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr Asn
            180                 185                 190         
Gln Glu Ala Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp Ser
        195                 200                 205             
Val Leu Trp Gly Gly His Lys Val Phe Met Asn Lys Pro Glu Glu Pro
    210                 215                 220                 
Phe Gln Pro Val Lys Glu Ala Thr Gln Leu Met Asn Glu Leu Val Tyr
225                 230                 235                 240 
Ser Gln
        


<210> 17
<211> 242
<212> PRT
<213> Potyvirus

<220> 
<221> VARIANT        
<222> 56
<223> L56V

<221> VARIANT        
<222> 135
<223> S135G

<221> VARIANT        
<222> 219
<223> S219N

<400> 17
Gly Glu Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Thr Ile Cys His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser Leu
            20                  25                  30          
Tyr Gly Ile Gly Phe Gly Pro Phe Ile Ile Thr Asn Lys His Leu Phe
        35                  40                  45              
Arg Arg Asn Asn Gly Thr Leu Val Val Gln Ser Leu His Gly Val Phe
    50                  55                  60                  
Lys Val Lys Asn Thr Thr Thr Leu Gln Gln His Leu Ile Asp Gly Arg
65                  70                  75                  80  
Asp Met Ile Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Lys Leu Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu Val
            100                 105                 110         
Thr Thr Asn Phe Gln Thr Lys Ser Met Ser Ser Met Val Ser Asp Thr
        115                 120                 125             
Ser Cys Thr Phe Pro Ser Gly Asp Gly Ile Phe Trp Lys His Trp Ile
    130                 135                 140                 
Gln Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg Asp
145                 150                 155                 160 
Gly Phe Ile Val Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr Asn
                165                 170                 175     
Asn Tyr Phe Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr Asn
            180                 185                 190         
Gln Glu Ala Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp Ser
        195                 200                 205             
Val Leu Trp Gly Gly His Lys Val Phe Met Asn Lys Pro Glu Glu Pro
    210                 215                 220                 
Phe Gln Pro Val Lys Glu Ala Thr Gln Leu Met Asn Glu Leu Val Tyr
225                 230                 235                 240 
Ser Gln
        


<210> 18
<211> 242
<212> PRT
<213> Potyvirus

<220> 
<221> VARIANT        
<222> 17
<223> T17V

<221> VARIANT        
<222> 68
<223> N68D

<221> VARIANT        
<222> 77
<223> I77V

<221> VARIANT        
<222> 219
<223> S219N

<400> 18
Gly Glu Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Ser Ile Cys His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser Leu
            20                  25                  30          
Tyr Gly Ile Gly Phe Gly Pro Phe Ile Ile Thr Asn Lys His Leu Phe
        35                  40                  45              
Arg Arg Asn Asn Gly Thr Leu Leu Val Gln Ser Leu His Gly Val Phe
    50                  55                  60                  
Lys Val Lys Asp Thr Thr Thr Leu Gln Gln His Leu Val Asp Gly Arg
65                  70                  75                  80  
Asp Met Ile Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Lys Leu Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu Val
            100                 105                 110         
Thr Thr Asn Phe Gln Thr Lys Ser Met Ser Ser Met Val Ser Asp Thr
        115                 120                 125             
Ser Cys Thr Phe Pro Ser Ser Asp Gly Ile Phe Trp Lys His Trp Ile
    130                 135                 140                 
Gln Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg Asp
145                 150                 155                 160 
Gly Phe Ile Val Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr Asn
                165                 170                 175     
Asn Tyr Phe Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr Asn
            180                 185                 190         
Gln Glu Ala Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp Ser
        195                 200                 205             
Val Leu Trp Gly Gly His Lys Val Phe Met Asn Lys Pro Glu Glu Pro
    210                 215                 220                 
Phe Gln Pro Val Lys Glu Ala Thr Gln Leu Met Asn Glu Leu Val Tyr
225                 230                 235                 240 
Ser Gln
        


<210> 19
<211> 242
<212> PRT
<213> Potyvirus

<220> 
<221> VARIANT        
<222> 44
<223> N44V

<221> VARIANT        
<222> 56
<223> L56V

<221> VARIANT        
<222> 135
<223> S135G

<221> VARIANT        
<222> 219
<223> S219N

<400> 19
Gly Glu Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Thr Ile Cys His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser Leu
            20                  25                  30          
Tyr Gly Ile Gly Phe Gly Pro Phe Ile Ile Thr Val Lys His Leu Phe
        35                  40                  45              
Arg Arg Asn Asn Gly Thr Leu Val Val Gln Ser Leu His Gly Val Phe
    50                  55                  60                  
Lys Val Lys Asn Thr Thr Thr Leu Gln Gln His Leu Ile Asp Gly Arg
65                  70                  75                  80  
Asp Met Ile Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Lys Leu Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu Val
            100                 105                 110         
Thr Thr Asn Phe Gln Thr Lys Ser Met Ser Ser Met Val Ser Asp Thr
        115                 120                 125             
Ser Cys Thr Phe Pro Ser Gly Asp Gly Ile Phe Trp Lys His Trp Ile
    130                 135                 140                 
Gln Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg Asp
145                 150                 155                 160 
Gly Phe Ile Val Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr Asn
                165                 170                 175     
Asn Tyr Phe Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr Asn
            180                 185                 190         
Gln Glu Ala Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp Ser
        195                 200                 205             
Val Leu Trp Gly Gly His Lys Val Phe Met Asn Lys Pro Glu Glu Pro
    210                 215                 220                 
Phe Gln Pro Val Lys Glu Ala Thr Gln Leu Met Asn Glu Leu Val Tyr
225                 230                 235                 240 
Ser Gln
        


<210> 20
<211> 242
<212> PRT
<213> Potyvirus

<220> 
<221> VARIANT        
<222> 56
<223> L56V

<221> VARIANT        
<222> 68
<223> N68D

<221> VARIANT        
<222> 135
<223> S135G

<221> VARIANT        
<222> 219
<223> S219N

<400> 20
Gly Glu Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Thr Ile Cys His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser Leu
            20                  25                  30          
Tyr Gly Ile Gly Phe Gly Pro Phe Ile Ile Thr Asn Lys His Leu Phe
        35                  40                  45              
Arg Arg Asn Asn Gly Thr Leu Val Val Gln Ser Leu His Gly Val Phe
    50                  55                  60                  
Lys Val Lys Asp Thr Thr Thr Leu Gln Gln His Leu Ile Asp Gly Arg
65                  70                  75                  80  
Asp Met Ile Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Lys Leu Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu Val
            100                 105                 110         
Thr Thr Asn Phe Gln Thr Lys Ser Met Ser Ser Met Val Ser Asp Thr
        115                 120                 125             
Ser Cys Thr Phe Pro Ser Gly Asp Gly Ile Phe Trp Lys His Trp Ile
    130                 135                 140                 
Gln Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg Asp
145                 150                 155                 160 
Gly Phe Ile Val Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr Asn
                165                 170                 175     
Asn Tyr Phe Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr Asn
            180                 185                 190         
Gln Glu Ala Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp Ser
        195                 200                 205             
Val Leu Trp Gly Gly His Lys Val Phe Met Asn Lys Pro Glu Glu Pro
    210                 215                 220                 
Phe Gln Pro Val Lys Glu Ala Thr Gln Leu Met Asn Glu Leu Val Tyr
225                 230                 235                 240 
Ser Gln
        


<210> 21
<211> 242
<212> PRT
<213> Potyvirus

<220> 
<221> VARIANT        
<222> 17
<223> T17S

<221> VARIANT        
<222> 56
<223> L56V

<221> VARIANT        
<222> 68
<223> N68D

<221> VARIANT        
<222> 77
<223> I77V

<221> VARIANT        
<222> 219
<223> S219N

<400> 21
Gly Glu Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Ser Ile Cys His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser Leu
            20                  25                  30          
Tyr Gly Ile Gly Phe Gly Pro Phe Ile Ile Thr Asn Lys His Leu Phe
        35                  40                  45              
Arg Arg Asn Asn Gly Thr Leu Val Val Gln Ser Leu His Gly Val Phe
    50                  55                  60                  
Lys Val Lys Asp Thr Thr Thr Leu Gln Gln His Leu Val Asp Gly Arg
65                  70                  75                  80  
Asp Met Ile Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Lys Leu Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu Val
            100                 105                 110         
Thr Thr Asn Phe Gln Thr Lys Ser Met Ser Ser Met Val Ser Asp Thr
        115                 120                 125             
Ser Cys Thr Phe Pro Ser Ser Asp Gly Ile Phe Trp Lys His Trp Ile
    130                 135                 140                 
Gln Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg Asp
145                 150                 155                 160 
Gly Phe Ile Val Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr Asn
                165                 170                 175     
Asn Tyr Phe Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr Asn
            180                 185                 190         
Gln Glu Ala Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp Ser
        195                 200                 205             
Val Leu Trp Gly Gly His Lys Val Phe Met Asn Lys Pro Glu Glu Pro
    210                 215                 220                 
Phe Gln Pro Val Lys Glu Ala Thr Gln Leu Met Asn Glu Leu Val Tyr
225                 230                 235                 240 
Ser Gln
        


<210> 22
<211> 242
<212> PRT
<213> Potyvirus

<220> 
<221> VARIANT        
<222> 17
<223> T17S

<221> VARIANT        
<222> 68
<223> N68D

<221> VARIANT        
<222> 77
<223> I77V

<221> VARIANT        
<222> 135
<223> S135G

<221> VARIANT        
<222> 219
<223> S219N

<400> 22
Gly Glu Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Ser Ile Cys His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser Leu
            20                  25                  30          
Tyr Gly Ile Gly Phe Gly Pro Phe Ile Ile Thr Asn Lys His Leu Phe
        35                  40                  45              
Arg Arg Asn Asn Gly Thr Leu Leu Val Gln Ser Leu His Gly Val Phe
    50                  55                  60                  
Lys Val Lys Asp Thr Thr Thr Leu Gln Gln His Leu Val Asp Gly Arg
65                  70                  75                  80  
Asp Met Ile Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Lys Leu Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu Val
            100                 105                 110         
Thr Thr Asn Phe Gln Thr Lys Ser Met Ser Ser Met Val Ser Asp Thr
        115                 120                 125             
Ser Cys Thr Phe Pro Ser Gly Asp Gly Ile Phe Trp Lys His Trp Ile
    130                 135                 140                 
Gln Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg Asp
145                 150                 155                 160 
Gly Phe Ile Val Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr Asn
                165                 170                 175     
Asn Tyr Phe Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr Asn
            180                 185                 190         
Gln Glu Ala Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp Ser
        195                 200                 205             
Val Leu Trp Gly Gly His Lys Val Phe Met Asn Lys Pro Glu Glu Pro
    210                 215                 220                 
Phe Gln Pro Val Lys Glu Ala Thr Gln Leu Met Asn Glu Leu Val Tyr
225                 230                 235                 240 
Ser Gln
        


<210> 23
<211> 242
<212> PRT
<213> Potyvirus

<220> 
<221> VARIANT        
<222> 17
<223> T17S

<221> VARIANT        
<222> 44
<223> N44V

<221> VARIANT        
<222> 56
<223> L56V

<221> VARIANT        
<222> 68
<223> N68D

<221> VARIANT        
<222> 77
<223> I77V

<221> VARIANT        
<222> 135
<223> S135G

<221> VARIANT        
<222> (219)...(219)
<223> S219N

<400> 23
Gly Glu Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Ser Ile Cys His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser Leu
            20                  25                  30          
Tyr Gly Ile Gly Phe Gly Pro Phe Ile Ile Thr Val Lys His Leu Phe
        35                  40                  45              
Arg Arg Asn Asn Gly Thr Leu Val Val Gln Ser Leu His Gly Val Phe
    50                  55                  60                  
Lys Val Lys Asp Thr Thr Thr Leu Gln Gln His Leu Val Asp Gly Arg
65                  70                  75                  80  
Asp Met Ile Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Lys Leu Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu Val
            100                 105                 110         
Thr Thr Asn Phe Gln Thr Lys Ser Met Ser Ser Met Val Ser Asp Thr
        115                 120                 125             
Ser Cys Thr Phe Pro Ser Gly Asp Gly Ile Phe Trp Lys His Trp Ile
    130                 135                 140                 
Gln Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg Asp
145                 150                 155                 160 
Gly Phe Ile Val Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr Asn
                165                 170                 175     
Asn Tyr Phe Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr Asn
            180                 185                 190         
Gln Glu Ala Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp Ser
        195                 200                 205             
Val Leu Trp Gly Gly His Lys Val Phe Met Asn Lys Pro Glu Glu Pro
    210                 215                 220                 
Phe Gln Pro Val Lys Glu Ala Thr Gln Leu Met Asn Glu Leu Val Tyr
225                 230                 235                 240 
Ser Gln
        


<210> 24
<211> 3023
<212> PRT
<213> Potyvirus

<400> 24
Met Ala Ala Thr Met Ile Phe Gly Ser Phe Thr His Asp Leu Leu Gly
 1               5                  10                  15      
Lys Ala Met Ser Thr Ile His Ser Ala Val Thr Ala Glu Lys Asp Ile
            20                  25                  30          
Phe Ser Ser Ile Lys Glu Arg Leu Glu Arg Lys Arg His Gly Lys Ile
        35                  40                  45              
Cys Arg Met Lys Asn Gly Ser Ile Tyr Ile Lys Ala Ala Ser Ser Thr
    50                  55                  60                  
Lys Val Glu Lys Ile Asn Ala Ala Ala Lys Lys Leu Ala Asp Asp Lys
65                  70                  75                  80  
Ala Ala Phe Leu Lys Ala Gln Pro Thr Ile Val Asp Lys Ile Ile Val
                85                  90                  95      
Asn Glu Lys Ile Gln Val Val Glu Ala Glu Glu Val His Lys Arg Glu
            100                 105                 110         
Asp Val Gln Thr Val Phe Phe Lys Lys Thr Lys Lys Arg Ala Pro Lys
        115                 120                 125             
Leu Arg Ala Thr Cys Ser Ser Ser Gly Leu Asp Asn Leu Tyr Asn Ala
    130                 135                 140                 
Val Ala Asn Ile Ala Lys Ala Ser Ser Leu Arg Val Glu Val Ile His
145                 150                 155                 160 
Lys Lys Arg Val Cys Gly Glu Phe Lys Gln Thr Arg Phe Gly Arg Ala
                165                 170                 175     
Leu Phe Ile Asp Val Ala His Ala Lys Gly His Arg Arg Arg Ile Asp
            180                 185                 190         
Cys Arg Met His Arg Arg Glu Gln Arg Thr Met His Met Phe Met Arg
        195                 200                 205             
Lys Thr Thr Lys Thr Glu Val Arg Ser Lys His Leu Arg Lys Gly Asp
    210                 215                 220                 
Ser Gly Ile Val Leu Leu Thr Gln Lys Ile Lys Gly His Leu Ser Gly
225                 230                 235                 240 
Val Arg Asp Glu Phe Phe Ile Val Arg Gly Thr Cys Asp Asp Ser Leu
                245                 250                 255     
Leu Glu Ala Arg Ala Arg Phe Ser Gln Ser Ile Thr Leu Arg Ala Thr
            260                 265                 270         
His Phe Ser Thr Gly Asp Ile Phe Trp Lys Gly Phe Asn Ala Ser Phe
        275                 280                 285             
Gln Glu Gln Lys Ala Ile Gly Leu Asp His Thr Cys Thr Ser Asp Leu
    290                 295                 300                 
Pro Val Glu Ala Cys Gly His Val Ala Ala Leu Met Cys Gln Ser Leu
305                 310                 315                 320 
Phe Pro Cys Gly Lys Ile Thr Cys Lys Arg Cys Ile Ala Asn Leu Ser
                325                 330                 335     
Asn Leu Asp Phe Asp Thr Phe Ser Glu Leu Gln Gly Asp Arg Ala Met
            340                 345                 350         
Arg Ile Leu Asp Val Met Arg Ala Arg Phe Pro Ser Phe Thr His Thr
        355                 360                 365             
Ile Arg Phe Leu His Asp Leu Phe Thr Gln Arg Arg Val Thr Asn Pro
    370                 375                 380                 
Asn Thr Ala Ala Phe Arg Glu Ile Leu Arg Leu Ile Gly Asp Arg Asn
385                 390                 395                 400 
Glu Ala Pro Phe Ala His Val Asn Arg Leu Asn Glu Ile Leu Leu Leu
                405                 410                 415     
Gly Ser Lys Ala Asn Pro Asp Ser Leu Ala Lys Ala Ser Asp Ser Leu
            420                 425                 430         
Leu Glu Leu Ala Arg Tyr Leu Asn Asn Arg Thr Glu Asn Ile Arg Asn
        435                 440                 445             
Gly Ser Leu Lys His Phe Arg Asn Lys Ile Ser Ser Lys Ala His Ser
    450                 455                 460                 
Asn Leu Ala Leu Ser Cys Asp Asn Gln Leu Asp Gln Asn Gly Asn Phe
465                 470                 475                 480 
Leu Trp Gly Leu Ala Gly Ile Ala Ala Lys Arg Phe Leu Asn Asn Tyr
                485                 490                 495     
Phe Glu Thr Ile Asp Pro Glu Gln Gly Tyr Asp Lys Tyr Val Ile Arg
            500                 505                 510         
Lys Asn Pro Asn Gly Glu Arg Lys Leu Ala Ile Gly Asn Phe Ile Ile
        515                 520                 525             
Ser Thr Asn Leu Glu Lys Leu Arg Asp Gln Leu Glu Gly Glu Ser Ile
    530                 535                 540                 
Ala Arg Val Gly Ile Thr Glu Glu Cys Val Ser Arg Lys Asp Gly Asn
545                 550                 555                 560 
Tyr Arg Tyr Pro Cys Cys Cys Val Thr Leu Glu Asp Gly Ser Pro Met
                565                 570                 575     
Tyr Ser Glu Leu Lys Met Pro Thr Lys Asn His Leu Val Ile Gly Asn
            580                 585                 590         
Ser Gly Asp Pro Lys Tyr Leu Asp Leu Pro Gly Glu Ile Ser Asn Leu
        595                 600                 605             
Met Tyr Ile Ala Lys Glu Gly Tyr Cys Tyr Ile Asn Ile Phe Leu Ala
    610                 615                 620                 
Met Leu Val Asn Val Asp Glu Ala Asn Ala Lys Asp Phe Thr Lys Arg
625                 630                 635                 640 
Val Arg Asp Glu Ser Val Gln Lys Leu Gly Lys Trp Pro Ser Leu Ile
                645                 650                 655     
Asp Val Ala Thr Glu Cys Ala Leu Leu Ser Thr Tyr Tyr Pro Ala Ala
            660                 665                 670         
Ala Ser Ala Glu Leu Pro Arg Leu Leu Val Asp His Ala Gln Lys Thr
        675                 680                 685             
Ile His Val Val Asp Ser Tyr Gly Ser Leu Asn Thr Gly Tyr His Ile
    690                 695                 700                 
Leu Lys Ala Asn Thr Val Ser Gln Leu Glu Lys Phe Ala Ser Asn Thr
705                 710                 715                 720 
Leu Glu Ser Pro Met Ala Gln Tyr Lys Val Gly Gly Leu Val Tyr Ser
                725                 730                 735     
Glu Asn Asn Asp Ala Ser Ala Val Lys Ala Leu Thr Gln Ala Ile Phe
            740                 745                 750         
Arg Pro Asp Val Leu Ser Glu Leu Ile Glu Lys Glu Pro Tyr Leu Met
        755                 760                 765             
Val Phe Ala Leu Val Ser Pro Gly Ile Leu Met Ala Met Ser Asn Ser
    770                 775                 780                 
Gly Ala Leu Glu Phe Gly Ile Ser Lys Trp Ile Ser Ser Asp His Ser
785                 790                 795                 800 
Leu Val Arg Met Ala Ser Ile Leu Lys Thr Leu Ala Ser Lys Val Ser
                805                 810                 815     
Val Ala Asp Thr Leu Ala Leu Gln Lys His Ile Met Arg Gln Asn Ala
            820                 825                 830         
Asn Phe Leu Cys Gly Glu Leu Ile Asn Gly Phe Gln Lys Lys Lys Ser
        835                 840                 845             
Tyr Thr His Ala Thr Arg Phe Leu Leu Met Ile Ser Glu Glu Asn Glu
    850                 855                 860                 
Met Asp Asp Pro Val Leu Asn Ala Gly Tyr Arg Val Leu Glu Ala Ser
865                 870                 875                 880 
Ser His Glu Ile Met Glu Lys Thr Tyr Leu Ala Leu Leu Glu Thr Ser
                885                 890                 895     
Trp Ser Asp Leu Ser Leu Tyr Gly Lys Phe Lys Ser Ile Trp Phe Thr
            900                 905                 910         
Arg Lys His Phe Gly Arg Tyr Lys Ala Glu Leu Phe Pro Lys Glu Gln
        915                 920                 925             
Thr Asp Leu Gln Gly Arg Tyr Ser Asn Ser Leu Arg Phe His Tyr Gln
    930                 935                 940                 
Ser Thr Leu Lys Arg Leu Arg Asn Lys Gly Ser Leu Cys Arg Glu Arg
945                 950                 955                 960 
Phe Leu Glu Ser Ile Ser Ser Ala Arg Arg Arg Thr Thr Cys Ala Val
                965                 970                 975     
Phe Ser Leu Leu His Lys Ala Phe Pro Asp Val Leu Lys Phe Ile Asn
            980                 985                 990         
Thr Leu Val Ile Val Ser Leu Ser Met Gln Ile Tyr Tyr Met Leu Val
        995                 1000                1005            
Ala Ile Ile His Glu His Arg Ala Ala Lys Ile Lys Ser Ala Gln Leu
    1010                1015                1020                
Glu Glu Arg Val Leu Glu Asp Lys Thr Met Leu Leu Tyr Asp Asp Phe
1025                1030                1035                1040
Lys Ala Lys Leu Pro Glu Gly Ser Phe Glu Glu Phe Leu Glu Tyr Thr
                1045                1050                1055    
Arg Gln Arg Asp Lys Glu Val Tyr Glu Tyr Leu Met Met Glu Thr Thr
            1060                1065                1070        
Glu Ile Val Glu Phe Gln Ala Lys Asn Thr Gly Gln Ala Ser Leu Glu
        1075                1080                1085            
Arg Ile Ile Ala Phe Val Ser Leu Thr Leu Met Leu Phe Asp Asn Glu
    1090                1095                1100                
Arg Ser Asp Cys Val Tyr Lys Ile Leu Thr Lys Phe Lys Gly Ile Leu
1105                1110                1115                1120
Gly Ser Val Glu Asn Asn Val Arg Phe Gln Ser Leu Asp Thr Ile Val
                1125                1130                1135    
Pro Thr Gln Glu Glu Lys Asn Met Val Ile Asp Phe Glu Leu Asp Ser
            1140                1145                1150        
Asp Thr Ala His Thr Pro Gln Met Gln Glu Gln Thr Phe Ser Asp Trp
        1155                1160                1165            
Trp Ser Asn Gln Ile Ala Asn Asn Arg Val Val Pro His Tyr Arg Thr
    1170                1175                1180                
Glu Gly Tyr Phe Met Gln Phe Thr Arg Asn Thr Ala Ser Ala Val Ser
1185                1190                1195                1200
His Gln Ile Ala His Asn Glu His Lys Asp Ile Ile Leu Met Gly Ala
                1205                1210                1215    
Val Gly Ser Gly Lys Ser Thr Gly Leu Pro Thr Asn Leu Cys Lys Phe
            1220                1225                1230        
Gly Gly Val Leu Leu Leu Glu Pro Thr Arg Pro Leu Ala Glu Asn Val
        1235                1240                1245            
Thr Lys Gln Met Arg Gly Ser Pro Phe Phe Ala Ser Pro Thr Leu Arg
    1250                1255                1260                
Met Arg Asn Leu Ser Thr Phe Gly Ser Ser Pro Ile Thr Val Met Thr
1265                1270                1275                1280
Thr Gly Phe Ala Leu His Phe Phe Ala Asn Asn Val Lys Glu Phe Asp
                1285                1290                1295    
Arg Tyr Gln Phe Ile Ile Phe Asp Glu Phe His Val Leu Asp Ser Asn
            1300                1305                1310        
Ala Ile Ala Phe Arg Asn Leu Cys His Glu Tyr Ser Tyr Asn Gly Lys
        1315                1320                1325            
Ile Ile Lys Val Ser Ala Thr Pro Pro Gly Arg Glu Cys Asp Leu Thr
    1330                1335                1340                
Thr Gln Tyr Pro Val Glu Leu Leu Ile Glu Glu Gln Leu Ser Leu Arg
1345                1350                1355                1360
Asp Phe Val Asp Ala Gln Gly Thr Asp Ala His Ala Asp Val Val Lys
                1365                1370                1375    
Lys Gly Asp Asn Ile Leu Val Tyr Val Ala Ser Tyr Asn Glu Val Asp
            1380                1385                1390        
Gln Leu Ser Lys Met Leu Asn Glu Arg Gly Phe Leu Val Thr Lys Val
        1395                1400                1405            
Asp Gly Arg Thr Met Lys Leu Gly Gly Val Glu Ile Ile Thr Lys Gly
    1410                1415                1420                
Ser Ser Ile Lys Lys His Phe Ile Val Ala Thr Asn Ile Ile Glu Asn
1425                1430                1435                1440
Gly Val Thr Leu Asp Val Asp Val Val Val Asp Phe Gly Leu Lys Val
                1445                1450                1455    
Val Pro Asn Leu Asp Ser Asp Asn Arg Leu Val Ser Tyr Cys Lys Ile
            1460                1465                1470        
Pro Ile Ser Leu Gly Glu Arg Ile Gln Arg Phe Gly Arg Val Gly Arg
        1475                1480                1485            
Asn Lys Pro Gly Val Ala Leu Arg Ile Gly Glu Thr Ile Lys Gly Leu
    1490                1495                1500                
Val Glu Ile Pro Ser Met Ile Ala Thr Glu Ala Ala Phe Leu Cys Phe
1505                1510                1515                1520
Val Tyr Gly Leu Pro Val Thr Thr Gln Asn Val Ser Thr Ser Ile Leu
                1525                1530                1535    
Ser Gln Val Ser Val Arg Gln Ala Arg Val Met Cys Gln Phe Glu Leu
            1540                1545                1550        
Pro Ile Phe Tyr Thr Ala His Leu Val Arg Tyr Asp Gly Ala Met His
        1555                1560                1565            
Pro Ala Ile His Asn Ala Leu Lys Arg Phe Lys Leu Arg Asp Ser Glu
    1570                1575                1580                
Ile Asn Leu Asn Thr Leu Ala Ile Pro Thr Ser Ser Ser Lys Thr Trp
1585                1590                1595                1600
Tyr Thr Gly Lys Cys Tyr Lys Gln Leu Val Gly Arg Leu Asp Ile Pro
                1605                1610                1615    
Asp Glu Ile Lys Ile Pro Phe Tyr Thr Lys Glu Val Pro Glu Lys Val
            1620                1625                1630        
Pro Glu Gln Ile Trp Asp Val Met Val Lys Phe Ser Ser Asp Ala Gly
        1635                1640                1645            
Phe Gly Arg Met Thr Ser Ala Ala Ala Cys Lys Val Ala Tyr Thr Leu
    1650                1655                1660                
Gln Thr Asp Ile His Ser Ile Gln Arg Thr Val Gln Ile Ile Asp Arg
1665                1670                1675                1680
Leu Leu Glu Asn Glu Met Lys Lys Arg Asn His Phe Asn Leu Val Val
                1685                1690                1695    
Asn Gln Ser Cys Ser Ser His Phe Met Ser Leu Ser Ser Ile Met Ala
            1700                1705                1710        
Ser Leu Arg Ala His Tyr Ala Lys Asn His Thr Gly Gln Asn Ile Glu
        1715                1720                1725            
Ile Leu Gln Lys Ala Lys Ala Gln Leu Leu Glu Phe Ser Asn Leu Ala
    1730                1735                1740                
Ile Asp Pro Ser Thr Thr Glu Ala Leu Arg Asp Phe Gly Tyr Leu Glu
1745                1750                1755                1760
Ala Val Arg Phe Gln Ser Glu Ser Glu Met Ala Arg Gly Leu Lys Leu
                1765                1770                1775    
Ser Gly His Trp Lys Trp Ser Leu Ile Ser Arg Asp Leu Ile Val Val
            1780                1785                1790        
Ser Gly Val Gly Ile Gly Leu Gly Cys Met Leu Trp Gln Phe Phe Lys
        1795                1800                1805            
Glu Lys Met His Glu Pro Val Lys Phe Gln Gly Lys Ser Arg Arg Arg
    1810                1815                1820                
Leu Gln Phe Arg Lys Ala Arg Asp Asp Lys Met Gly Tyr Ile Met His
1825                1830                1835                1840
Gly Glu Gly Asp Thr Ile Glu His Phe Phe Gly Ala Ala Tyr Thr Lys
                1845                1850                1855    
Lys Gly Lys Ser Lys Gly Lys Thr His Gly Ala Gly Thr Lys Ala His
            1860                1865                1870        
Lys Phe Val Asn Met Tyr Gly Val Ser Pro Asp Glu Tyr Ser Tyr Val
        1875                1880                1885            
Arg Tyr Leu Asp Pro Val Thr Gly Ala Thr Leu Asp Glu Ser Pro Met
    1890                1895                1900                
Thr Asp Leu Asn Ile Val Gln Glu His Phe Gly Glu Ile Arg Arg Glu
1905                1910                1915                1920
Ala Ile Leu Ala Asp Ala Met Ser Pro Gln Gln Arg Asn Lys Gly Ile
                1925                1930                1935    
Gln Ala Tyr Phe Val Arg Asn Ser Thr Met Pro Ile Leu Lys Val Asp
            1940                1945                1950        
Leu Thr Pro His Ile Pro Leu Lys Val Cys Glu Ser Asn Asn Ile Ala
        1955                1960                1965            
Gly Phe Pro Glu Arg Glu Gly Glu Leu Arg Arg Thr Gly Pro Thr Glu
    1970                1975                1980                
Thr Leu Pro Phe Asp Ala Leu Pro Pro Glu Lys Gln Glu Val Ala Phe
1985                1990                1995                2000
Glu Ser Lys Ala Leu Leu Lys Gly Val Arg Asp Phe Asn Pro Ile Ser
                2005                2010                2015    
Ala Cys Val Trp Leu Leu Glu Asn Ser Ser Asp Gly His Ser Glu Arg
            2020                2025                2030        
Leu Phe Gly Ile Gly Phe Gly Pro Tyr Ile Ile Ala Asn Gln His Leu
        2035                2040                2045            
Phe Arg Arg Asn Asn Gly Glu Leu Thr Ile Lys Thr Met His Gly Glu
    2050                2055                2060                
Phe Lys Val Lys Asn Ser Thr Gln Leu Gln Met Lys Pro Val Glu Gly
2065                2070                2075                2080
Arg Asp Ile Ile Val Ile Lys Met Ala Lys Asp Phe Pro Pro Phe Pro
                2085                2090                2095    
Gln Lys Leu Lys Phe Arg Gln Pro Thr Ile Lys Asp Arg Val Cys Met
            2100                2105                2110        
Val Ser Thr Asn Phe Gln Gln Lys Ser Val Ser Ser Leu Val Ser Glu
        2115                2120                2125            
Ser Ser His Ile Val His Lys Glu Asp Thr Ser Phe Trp Gln His Trp
    2130                2135                2140                
Ile Thr Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Ile Ile
2145                2150                2155                2160
Asp Gly Asn Ile Leu Gly Ile His Ser Leu Thr His Thr Thr Asn Gly
                2165                2170                2175    
Ser Asn Tyr Phe Val Glu Phe Pro Glu Lys Phe Val Ala Thr Tyr Leu
            2180                2185                2190        
Asp Ala Ala Asp Gly Trp Cys Lys Asn Trp Lys Phe Asn Ala Asp Lys
        2195                2200                2205            
Ile Ser Trp Gly Ser Phe Thr Leu Val Glu Asp Ala Pro Glu Asp Asp
    2210                2215                2220                
Phe Met Ala Lys Lys Thr Val Ala Ala Ile Met Asp Asp Leu Val Arg
2225                2230                2235                2240
Thr Gln Gly Glu Lys Arg Lys Trp Met Leu Glu Ala Ala His Thr Asn
                2245                2250                2255    
Ile Gln Pro Val Ala His Leu Gln Ser Gln Leu Val Thr Lys His Ile
            2260                2265                2270        
Val Lys Gly Arg Cys Lys Met Phe Ala Leu Tyr Leu Gln Glu Asn Ala
        2275                2280                2285            
Asp Ala Arg Asp Phe Phe Lys Ser Phe Met Gly Ala Tyr Gly Pro Ser
    2290                2295                2300                
His Leu Asn Lys Glu Ala Tyr Ile Lys Asp Ile Met Lys Tyr Ser Lys
2305                2310                2315                2320
Gln Ile Val Val Gly Ser Val Asp Cys Asp Thr Phe Glu Ser Ser Leu
                2325                2330                2335    
Lys Val Leu Ser Arg Lys Met Lys Glu Trp Gly Phe Glu Asn Leu Glu
            2340                2345                2350        
Tyr Val Thr Asp Glu Gln Thr Ile Lys Asn Ala Leu Asn Met Asp Ala
        2355                2360                2365            
Ala Val Gly Ala Leu Tyr Ser Gly Lys Lys Lys Gln Tyr Phe Glu Asp
    2370                2375                2380                
Leu Ser Asp Asp Ala Val Ala Asn Leu Val Gln Lys Ser Cys Leu Arg
2385                2390                2395                2400
Leu Phe Lys Asn Lys Leu Gly Val Trp Asn Gly Ser Leu Lys Ala Glu
                2405                2410                2415    
Leu Arg Pro Phe Glu Lys Leu Ile Glu Asn Lys Thr Arg Thr Phe Thr
            2420                2425                2430        
Ala Ala Pro Ile Glu Thr Leu Leu Gly Gly Lys Val Cys Val Asp Asp
        2435                2440                2445            
Phe Asn Asn His Phe Tyr Ser Lys His Ile Gln Cys Pro Trp Ser Val
    2450                2455                2460                
Gly Met Thr Lys Phe Tyr Gly Gly Trp Asn Glu Leu Leu Gly Lys Leu
2465                2470                2475                2480
Pro Asp Gly Trp Val Tyr Cys Asp Ala Asp Gly Ser Gln Phe Asp Ser
                2485                2490                2495    
Ser Leu Ser Pro Tyr Leu Ile Asn Ala Val Leu Arg Leu Arg Leu Ser
            2500                2505                2510        
Ser Met Glu Glu Trp Asp Val Gly Gln Lys Met Leu Gln Asn Leu Tyr
        2515                2520                2525            
Thr Glu Ile Val Tyr Thr Pro Ile Ser Thr Pro Asp Gly Thr Ile Val
    2530                2535                2540                
Lys Lys Phe Lys Gly Asn Asn Ser Gly Gln Pro Ser Thr Val Val Asp
2545                2550                2555                2560
Asn Thr Leu Met Val Val Leu Ala Met Tyr Tyr Ala Leu Ser Lys Leu
                2565                2570                2575    
Gly Val Asp Ile Asn Ser Gln Glu Asp Val Cys Lys Phe Phe Ala Asn
            2580                2585                2590        
Gly Asp Asp Leu Ile Ile Ala Ile Ser Pro Glu Leu Glu His Val Leu
        2595                2600                2605            
Asp Gly Phe Gln Gln His Phe Ser Asp Leu Gly Leu Asn Tyr Asp Phe
    2610                2615                2620                
Ser Ser Arg Thr Arg Asp Lys Lys Glu Leu Trp Phe Met Ser His Arg
2625                2630                2635                2640
Ala Leu Ser Lys Asp Gly Ile Leu Ile Pro Lys Leu Glu Pro Glu Arg
                2645                2650                2655    
Ile Val Ser Ile Leu Glu Trp Asp Arg Ser Ala Glu Pro His His Arg
            2660                2665                2670        
Leu Glu Ala Ile Cys Ala Ser Met Ile Glu Ala Trp Gly Tyr Thr Asp
        2675                2680                2685            
Leu Leu Gln Asn Ile Arg Arg Phe Tyr Lys Trp Thr Ile Glu Gln Glu
    2690                2695                2700                
Pro Tyr Arg Ser Leu Ala Glu Gln Gly Leu Ala Pro Tyr Leu Ser Glu
2705                2710                2715                2720
Val Ala Leu Arg Arg Leu Tyr Thr Ser Gln Ile Ala Thr Asp Asn Glu
                2725                2730                2735    
Leu Thr Asp Tyr Tyr Lys Glu Ile Leu Ala Asn Asn Glu Phe Leu Arg
            2740                2745                2750        
Glu Thr Val Arg Phe Gln Ser Asp Thr Val Asp Ala Gly Lys Asp Lys
        2755                2760                2765            
Ala Arg Asp Gln Lys Leu Ala Asp Lys Pro Thr Leu Ala Ile Asp Arg
    2770                2775                2780                
Thr Lys Asp Lys Asp Val Asn Thr Gly Thr Ser Gly Thr Phe Ser Ile
2785                2790                2795                2800
Pro Arg Leu Lys Lys Ala Ala Met Asn Met Lys Leu Pro Lys Val Gly
                2805                2810                2815    
Gly Ser Ser Val Val Asn Leu Asp His Leu Leu Thr Tyr Lys Pro Ala
            2820                2825                2830        
Gln Glu Phe Val Val Asn Thr Arg Ala Thr His Ser Gln Phe Lys Ala
        2835                2840                2845            
Trp His Thr Asn Val Met Ala Glu Leu Glu Leu Asn Glu Glu Gln Met
    2850                2855                2860                
Lys Ile Val Leu Asn Gly Phe Met Ile Trp Cys Ile Glu Asn Gly Thr
2865                2870                2875                2880
Ser Pro Asn Ile Ser Gly Val Trp Thr Met Met Asp Gly Asp Glu Gln
                2885                2890                2895    
Val Glu Tyr Pro Ile Glu Pro Met Val Lys His Ala Asn Pro Ser Leu
            2900                2905                2910        
Arg Gln Ile Met Lys His Phe Ser Asn Leu Ala Glu Ala Tyr Ile Arg
        2915                2920                2925            
Met Arg Asn Ser Glu Gln Val Tyr Ile Pro Arg Tyr Gly Leu Gln Arg
    2930                2935                2940                
Gly Leu Val Asp Arg Asn Leu Ala Pro Phe Ala Phe Asp Phe Phe Glu
2945                2950                2955                2960
Val Asn Gly Ala Thr Pro Val Arg Ala Arg Glu Ala His Ala Gln Met
                2965                2970                2975    
Lys Ala Ala Ala Leu Arg Asn Ser Gln Gln Arg Met Phe Cys Leu Asp
            2980                2985                2990        
Gly Ser Val Ser Gly Gln Glu Glu Asn Thr Glu Arg His Thr Val Asp
        2995                3000                3005            
Asp Val Asn Ala Gln Met His His Leu Leu Gly Val Lys Gly Val
    3010                3015                3020            


<210> 25
<211> 381
<212> PRT
<213> Bacillus subtilis

<400> 25
Met Arg Gly Lys Lys Val Trp Ile Ser Leu Leu Phe Ala Leu Thr Leu
 1               5                  10                  15      
Ile Phe Thr Met Ala Phe Ser Asn Met Ser Ala Gln Ala Ala Gly Lys
            20                  25                  30          
Ser Ser Thr Glu Lys Lys Tyr Ile Val Gly Phe Lys Gln Thr Met Ser
        35                  40                  45              
Ala Met Ser Ser Ala Lys Lys Lys Asp Val Ile Ser Glu Lys Gly Gly
    50                  55                  60                  
Lys Val Gln Lys Gln Phe Lys Tyr Val Asn Ala Ala Thr Ala Thr Leu
65                  70                  75                  80  
Asp Glu Lys Ala Val Lys Glu Leu Lys Gln Asp Pro Ser Val Ala Tyr
                85                  90                  95      
Val Glu Glu Asp His Ile Ala His Glu Tyr Ala Gln Ser Val Pro Tyr
            100                 105                 110         
Gly Ile Ser Gln Ile Lys Ala Pro Ala Leu His Ser Gln Gly Tyr Thr
        115                 120                 125             
Gly Ser Asn Val Lys Val Ala Val Ile Asp Ser Gly Ile Asp Ser Ser
    130                 135                 140                 
His Pro Asp Leu Asn Val Lys Gly Gly Ala Ser Phe Val Pro Ser Glu
145                 150                 155                 160 
Thr Asn Pro Tyr Gln Asp Gly Ser Ser His Gly Thr His Val Ala Gly
                165                 170                 175     
Thr Ile Ala Ala Leu Asn Asn Thr Ile Gly Val Leu Gly Val Ala Pro
            180                 185                 190         
Asn Ala Ser Leu Tyr Ala Val Lys Val Leu Asp Ser Thr Gly Ser Gly
        195                 200                 205             
Gln Tyr Ser Trp Ile Ile Asn Gly Ile Glu Trp Ala Ile Ser Asn Asn
    210                 215                 220                 
Met Asp Val Ile Asn Met Ser Leu Gly Gly Pro Ser Gly Ser Thr Ala
225                 230                 235                 240 
Leu Lys Thr Val Val Asp Lys Ala Val Ser Ser Gly Ile Val Val Ala
                245                 250                 255     
Ala Ala Ala Gly Asn Glu Gly Ser Ser Gly Ser Thr Ser Thr Val Gly
            260                 265                 270         
Tyr Pro Ala Lys Tyr Pro Ser Thr Ile Ala Val Gly Ala Val Asn Ser
        275                 280                 285             
Ser Asn Gln Arg Ala Ser Phe Ser Ser Ala Gly Ser Glu Leu Asp Val
    290                 295                 300                 
Met Ala Pro Gly Val Ser Ile Gln Ser Thr Leu Pro Gly Gly Thr Tyr
305                 310                 315                 320 
Gly Ala Tyr Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala
                325                 330                 335     
Ala Ala Leu Ile Leu Ser Lys His Pro Thr Trp Thr Asn Ala Gln Val
            340                 345                 350         
Arg Asp Arg Leu Glu Ser Thr Ala Thr Tyr Leu Gly Ser Ser Phe Tyr
        355                 360                 365             
Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala Ala Ala Gln
    370                 375                 380     


<210> 26
<211> 277
<212> PRT
<213> Rattus norvegicus

<400> 26
Met Asp Asn Asn Glu Thr Ser Val Asp Ser Lys Ser Ile Asn Asn Phe
 1               5                  10                  15      
Glu Thr Lys Thr Ile His Gly Ser Lys Ser Met Asp Ser Gly Ile Tyr
            20                  25                  30          
Leu Asp Ser Ser Tyr Lys Met Asp Tyr Pro Glu Met Gly Leu Cys Ile
        35                  40                  45              
Ile Ile Asn Asn Lys Asn Phe His Lys Ser Thr Gly Met Ser Ala Arg
    50                  55                  60                  
Asn Gly Thr Asp Val Asp Ala Ala Asn Leu Arg Glu Thr Phe Met Ala
65                  70                  75                  80  
Leu Lys Tyr Glu Val Arg Asn Lys Asn Asp Leu Thr Arg Glu Glu Ile
                85                  90                  95      
Met Glu Leu Met Asp Ser Val Ser Lys Glu Asp His Ser Lys Arg Ser
            100                 105                 110         
Ser Phe Val Cys Val Ile Leu Ser His Gly Asp Glu Gly Val Ile Phe
        115                 120                 125             
Gly Thr Asn Gly Pro Val Asp Leu Lys Lys Leu Thr Ser Phe Phe Arg
    130                 135                 140                 
Gly Asp Tyr Cys Arg Ser Leu Thr Gly Lys Pro Lys Leu Phe Ile Ile
145                 150                 155                 160 
Gln Ala Cys Arg Gly Thr Glu Leu Asp Cys Gly Ile Glu Thr Asp Ser
                165                 170                 175     
Gly Thr Asp Asp Asp Met Ala Cys Gln Lys Ile Pro Val Glu Ala Asp
            180                 185                 190         
Phe Leu Tyr Ala Tyr Ser Thr Ala Pro Gly Tyr Tyr Ser Trp Arg Asn
        195                 200                 205             
Ser Arg Asp Gly Ser Trp Phe Ile Gln Ser Leu Cys Ala Met Leu Lys
    210                 215                 220                 
Leu Tyr Ala His Lys Leu Glu Phe Met His Ile Leu Thr Arg Val Asn
225                 230                 235                 240 
Arg Lys Val Ala Thr Glu Phe Glu Ser Phe Ser Leu Asp Ala Thr Phe
                245                 250                 255     
His Ala Lys Lys Gln Ile Pro Cys Ile Val Ser Met Leu Thr Lys Glu
            260                 265                 270         
Leu Tyr Phe Tyr His
        275         


<210> 27
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tobacco Etch Virus protease cleavage site
      consensus sequence

<221> VARIANT        
<222> 2, 3, 5
<223> Xaa can be any amino acid

<400> 27
Glu Xaa Xaa Tyr Xaa Gln Gly
 1               5          


<210> 28
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tobacco Etch Virus protease cleavage site
      consensus sequence

<221> VARIANT        
<222> 2, 3, 5
<223> Xaa can be any amino acid

<400> 28
Glu Xaa Xaa Tyr Xaa Gln Ser
 1               5          


<210> 29
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tobacco Etch Virus protease cleavage site

<400> 29
Glu Asn Leu Tyr Phe Gln Gly
 1               5          


<210> 30
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tobacco Etch Virus protease cleavage site

<400> 30
Glu Asn Leu Tyr Phe Gln Ser
 1               5          


<210> 31
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tobacco Etch Virus protease cleavage site

<400> 31
Glu Asn Ile Tyr Thr Gln Gly
 1               5          


<210> 32
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tobacco Etch Virus protease cleavage site

<400> 32
Glu Asn Ile Tyr Thr Gln Ser
 1               5          


<210> 33
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tobacco Etch Virus protease cleavage site

<400> 33
Glu Asn Ile Tyr Leu Gln Gly
 1               5          


<210> 34
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tobacco Etch Virus protease cleavage site

<400> 34
Glu Asn Ile Tyr Leu Gln Ser
 1               5          


<210> 35
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tobacco Etch Virus protease cleavage site

<400> 35
Glu Asn Val Tyr Phe Gln Gly
 1               5          


<210> 36
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tobacco Etch Virus protease cleavage site

<400> 36
Glu Asn Val Tyr Ser Gln Ser
 1               5          


<210> 37
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tobacco Etch Virus protease cleavage site

<400> 37
Glu Asn Val Tyr Ser Gln Gly
 1               5          


<210> 38
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tobacco Etch Virus protease cleavage site

<400> 38
Glu Asn Val Tyr Ser Gln Ser
 1               5          


<210> 39
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tobacco Vein Mottling Virus protease cleavage site
      consensus sequence

<221> VARIANT        
<222> 1, 2
<223> Xaa can be any amino acid

<400> 39
Xaa Xaa Val Arg Phe Gln Gly
 1               5          


<210> 40
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tobacco Vein Mottling Virus protease cleavage site
      consensus sequence

<221> VARIANT        
<222> 1, 2
<223> Xaa can be any amino acid

<400> 40
Xaa Xaa Val Arg Phe Gln Ser
 1               5          


<210> 41
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tobacco Vein Mottling Virus protease cleavage site

<400> 41
Glu Thr Val Arg Phe Gln Gly
 1               5          


<210> 42
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tobacco Vein Mottling Virus protease cleavage site

<400> 42
Glu Thr Val Arg Phe Gln Ser
 1               5          


<210> 43
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tobacco Vein Mottling Virus protease cleavage site

<400> 43
Asn Asn Val Arg Phe Gln Gly
 1               5          


<210> 44
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tobacco Vein Mottling Virus protease cleavage site

<400> 44
Asn Asn Val Arg Phe Gln Ser
 1               5          


<210> 45
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human Rhinovirus 3C protease cleavage site
      consensus sequence

<221> VARIANT        
<222> 1
<223> Xaa can be amino acid, with D or E preferred

<221> VARIANT        
<222> 2
<223> Xaa can be G, A, V, L, I, M, S or T

<400> 45
Xaa Xaa Leu Phe Gln Gly Pro
 1               5          


<210> 46
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human Rhinovirus 3C protease cleavage site

<400> 46
Glu Ala Leu Phe Gln Gly Pro
 1               5          


<210> 47
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human Rhinovirus 3C protease cleavage site

<400> 47
Glu Val Leu Phe Gln Gly Pro
 1               5          


<210> 48
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human Rhinovirus 3C protease cleavage site

<400> 48
Glu Leu Leu Phe Gln Gly Pro
 1               5          


<210> 49
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human Rhinovirus 3C protease cleavage site

<400> 49
Asp Ala Leu Phe Gln Gly Pro
 1               5          


<210> 50
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human Rhinovirus 3C protease cleavage site

<400> 50
Asp Val Leu Phe Gln Gly Pro
 1               5          


<210> 51
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human Rhinovirus 3C protease cleavage site

<400> 51
Asp Leu Leu Phe Gln Gly Pro
 1               5          


<210> 52
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> Subtilisin protease cleavage site consensus
      sequence

<221> VARIANT        
<222> 1, 2, 3, 4
<223> Xaa can be any amino acid

<400> 52
Xaa Xaa Xaa Xaa His Tyr
 1               5      


<210> 53
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> Subtilisin protease cleavage site consensus
      sequence

<221> VARIANT        
<222> 1, 2, 3, 4
<223> Xaa can be any amino acid

<400> 53
Xaa Xaa Xaa Xaa His Tyr
 1               5      


<210> 54
<211> 2
<212> PRT
<213> Artificial Sequence

<220> 
<223> Subtilisin protease cleavage site

<400> 54
His Tyr
 1      


<210> 55
<211> 2
<212> PRT
<213> Artificial Sequence

<220> 
<223> Subtilisin protease cleavage site

<400> 55
Tyr His
 1      


<210> 56
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> Subtilisin protease cleavage site

<400> 56
Pro Gly Ala Ala His Tyr
 1               5      


<210> 57
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> Caspase 3 protease cleavage site consensus
      sequence

<221> VARIANT        
<222> 2
<223> Xaa can be any amino acid with E preferred

<221> VARIANT        
<222> 3
<223> Xaa can be any amino acid

<221> VARIANT        
<222> 5
<223> Xaa can be any amino acid with G or S preferred

<400> 57
Asp Xaa Xaa Asp Xaa
 1               5  


<210> 58
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> Caspase 3 protease cleavage site

<400> 58
Asp Glu Val Asp Gly
 1               5  


<210> 59
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> Caspase 3 protease cleavage site

<400> 59
Asp Glu Val Asp Ser
 1               5  


<210> 60
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> Caspase 3 protease cleavage site

<400> 60
Asp Glu Pro Asp Gly
 1               5  


<210> 61
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> Caspase 3 protease cleavage site

<400> 61
Asp Glu Pro Asp Ser
 1               5  


<210> 62
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> Caspase 3 protease cleavage site

<400> 62
Asp Glu Leu Asp Gly
 1               5  


<210> 63
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> Caspase 3 protease cleavage site

<400> 63
Asp Glu Leu Asp Ser
 1               5  


<210> 64
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> Enterokinase protease cleavage site consensus
      sequence

<400> 64
Asp Asp Asp Asp Lys
 1               5  


<210> 65
<211> 753
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon-optimized TEV open reading frame

<400> 65
atgggcgaat ctctgttcaa gggtccgcgt gattataacc cgatatcttc tactatttgt 60
catctgacta acgaaagcga cggccacacg acttctctgt acggtatcgg tttcggtccg 120
ttcatcatta ccaacaagca tctgttccgc cgtaacaacg gtaccctgct ggttcaatct 180
ctgcacggcg tcttcaaggt aaaaaatacc actacgctgc agcagcacct gattgacggc 240
cgtgacatga tcatcatccg catgccgaaa gattttccgc cgttcccgca aaaactgaag 300
tttcgtgaac cgcaacgcga agaacgtatt tgcctggtta ccaccaactt tcagaccaaa 360
agcatgtctt ctatggtttc cgatacctct tgcaccttcc caagctctga cggtattttc 420
tggaaacatt ggatccagac caaagatggt cagtgcggct ctccgctggt gtctacgcgt 480
gacggtttca tcgttggtat ccattctgct tctaacttca ctaacactaa caactacttt 540
acttccgttc cgaaaaactt catggagctg ctgactaacc aagaggccca gcagtgggtg 600
tccggttggc gcctgaacgc agattctgta ctgtggggtg gtcataaggt attcatgaac 660
aaaccggagg agccgttcca gccggtcaaa gaggcgaccc agctgatgaa cgaactggtt 720
tactctcagg gtcaccacca tcaccaccat taa                              753

<210> 66
<211> 250
<212> PRT
<213> Artificial Sequence

<220> 
<223> TEV protease (S219N) with amino-terminus
      polyhistidine affinity tag

<400> 66
Met Gly Glu Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser
 1               5                  10                  15      
Ser Thr Ile Cys His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser
            20                  25                  30          
Leu Tyr Gly Ile Gly Phe Gly Pro Phe Ile Ile Thr Asn Lys His Leu
        35                  40                  45              
Phe Arg Arg Asn Asn Gly Thr Leu Leu Val Gln Ser Leu His Gly Val
    50                  55                  60                  
Phe Lys Val Lys Asn Thr Thr Thr Leu Gln Gln His Leu Ile Asp Gly
65                  70                  75                  80  
Arg Asp Met Ile Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro
                85                  90                  95      
Gln Lys Leu Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu
            100                 105                 110         
Val Thr Thr Asn Phe Gln Thr Lys Ser Met Ser Ser Met Val Ser Asp
        115                 120                 125             
Thr Ser Cys Thr Phe Pro Ser Ser Asp Gly Ile Phe Trp Lys His Trp
    130                 135                 140                 
Ile Gln Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg
145                 150                 155                 160 
Asp Gly Phe Ile Val Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr
                165                 170                 175     
Asn Asn Tyr Phe Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr
            180                 185                 190         
Asn Gln Glu Ala Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp
        195                 200                 205             
Ser Val Leu Trp Gly Gly His Lys Val Phe Met Asn Lys Pro Glu Glu
    210                 215                 220                 
Pro Phe Gln Pro Val Lys Glu Ala Thr Gln Leu Met Asn Glu Leu Val
225                 230                 235                 240 
Tyr Ser Gln Gly His His His His His His
                245                 250 


<210> 67
<211> 753
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon-optimized TEV open reading frame

<400> 67
atgggtcacc accatcacca ccatggcgaa tctctgttca agggtccgcg tgattataac 60
ccgatatctt ctactatttg tcatctgact aacgaaagcg acggccacac gacttctctg 120
tacggtatcg gtttcggtcc gttcatcatt accaacaagc atctgttccg ccgtaacaac 180
ggtaccctgg tggttcaatc tctgcacggc gtcttcaagg taaaaaatac cactacgctg 240
cagcagcacc tgattgacgg ccgtgacatg atcatcatcc gcatgccgaa agattttccg 300
ccgttcccgc aaaaactgaa gtttcgtgaa ccgcaacgcg aagaacgtat ttgcctggtt 360
accaccaact ttcagaccaa aagcatgtct tctatggttt ccgatacctc ttgcaccttc 420
ccaagcggtg acggtatttt ctggaaacat tggatccaga ccaaagatgg tcagtgcggc 480
tctccgctgg tgtctacgcg tgacggtttc atcgttggta tccattctgc ttctaacttc 540
actaacacta acaactactt tacttccgtt ccgaaaaact tcatggagct gctgactaac 600
caagaggccc agcagtgggt gtccggttgg cgcctgaacg cagattctgt actgtggggt 660
ggtcataagg tattcatgaa caaaccggag gagccgttcc agccggtcaa agaggcgacc 720
cagctgatga acgaactggt ttactctcag taa                              753

<210> 68
<211> 250
<212> PRT
<213> Artificial Sequence

<220> 
<223> TEV protease (L56V, S135G, S219N) with
      amino-terminus polyhistidine affinity tag

<400> 68
Met Gly His His His His His His Gly Glu Ser Leu Phe Lys Gly Pro
 1               5                  10                  15      
Arg Asp Tyr Asn Pro Ile Ser Ser Thr Ile Cys His Leu Thr Asn Glu
            20                  25                  30          
Ser Asp Gly His Thr Thr Ser Leu Tyr Gly Ile Gly Phe Gly Pro Phe
        35                  40                  45              
Ile Ile Thr Asn Lys His Leu Phe Arg Arg Asn Asn Gly Thr Leu Val
    50                  55                  60                  
Val Gln Ser Leu His Gly Val Phe Lys Val Lys Asn Thr Thr Thr Leu
65                  70                  75                  80  
Gln Gln His Leu Ile Asp Gly Arg Asp Met Ile Ile Ile Arg Met Pro
                85                  90                  95      
Lys Asp Phe Pro Pro Phe Pro Gln Lys Leu Lys Phe Arg Glu Pro Gln
            100                 105                 110         
Arg Glu Glu Arg Ile Cys Leu Val Thr Thr Asn Phe Gln Thr Lys Ser
        115                 120                 125             
Met Ser Ser Met Val Ser Asp Thr Ser Cys Thr Phe Pro Ser Gly Asp
    130                 135                 140                 
Gly Ile Phe Trp Lys His Trp Ile Gln Thr Lys Asp Gly Gln Cys Gly
145                 150                 155                 160 
Ser Pro Leu Val Ser Thr Arg Asp Gly Phe Ile Val Gly Ile His Ser
                165                 170                 175     
Ala Ser Asn Phe Thr Asn Thr Asn Asn Tyr Phe Thr Ser Val Pro Lys
            180                 185                 190         
Asn Phe Met Glu Leu Leu Thr Asn Gln Glu Ala Gln Gln Trp Val Ser
        195                 200                 205             
Gly Trp Arg Leu Asn Ala Asp Ser Val Leu Trp Gly Gly His Lys Val
    210                 215                 220                 
Phe Met Asn Lys Pro Glu Glu Pro Phe Gln Pro Val Lys Glu Ala Thr
225                 230                 235                 240 
Gln Leu Met Asn Glu Leu Val Tyr Ser Gln
                245                 250 


<210> 69
<211> 753
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon-optimized TEV open reading frame

<400> 69
atgggtcacc accatcacca ccatggcgaa tctctgttca agggtccgcg tgattataac 60
ccgatatctt cttctatttg tcatctgact aacgaaagcg acggccacac gacttctctg 120
tacggtatcg gtttcggtcc gttcatcatt accaacaagc atctgttccg ccgtaacaac 180
ggtaccctgc tggttcaatc tctgcacggc gtcttcaagg taaaagacac cactacgctg 240
cagcagcacc tggtcgacgg ccgtgacatg atcatcatcc gcatgccgaa agattttccg 300
ccgttcccgc aaaaactgaa gtttcgtgaa ccgcaacgcg aagaacgtat ttgcctggtt 360
accaccaact ttcagaccaa aagcatgtct tctatggttt ccgatacctc ttgcaccttc 420
ccaagctctg acggtatttt ctggaaacat tggatccaga ccaaagatgg tcagtgcggc 480
tctccgctgg tgtctacgcg tgacggtttc atcgttggta tccattctgc ttctaacttc 540
actaacacta acaactactt tacttccgtt ccgaaaaact tcatggagct gctgactaac 600
caagaggccc agcagtgggt gtccggttgg cgcctgaacg cagattctgt actgtggggt 660
ggtcataagg tattcatgaa caaaccggag gagccgttcc agccggtcaa agaggcgacc 720
cagctgatga acgaactggt ttactctcag taa                              753

<210> 70
<211> 250
<212> PRT
<213> Artificial Sequence

<220> 
<223> TEV protease (T17S, N68D, I77V, S219N) with
      amino-terminus polyhistidine affinity tag

<400> 70
Met Gly His His His His His His Gly Glu Ser Leu Phe Lys Gly Pro
 1               5                  10                  15      
Arg Asp Tyr Asn Pro Ile Ser Ser Ser Ile Cys His Leu Thr Asn Glu
            20                  25                  30          
Ser Asp Gly His Thr Thr Ser Leu Tyr Gly Ile Gly Phe Gly Pro Phe
        35                  40                  45              
Ile Ile Thr Asn Lys His Leu Phe Arg Arg Asn Asn Gly Thr Leu Leu
    50                  55                  60                  
Val Gln Ser Leu His Gly Val Phe Lys Val Lys Asp Thr Thr Thr Leu
65                  70                  75                  80  
Gln Gln His Leu Val Asp Gly Arg Asp Met Ile Ile Ile Arg Met Pro
                85                  90                  95      
Lys Asp Phe Pro Pro Phe Pro Gln Lys Leu Lys Phe Arg Glu Pro Gln
            100                 105                 110         
Arg Glu Glu Arg Ile Cys Leu Val Thr Thr Asn Phe Gln Thr Lys Ser
        115                 120                 125             
Met Ser Ser Met Val Ser Asp Thr Ser Cys Thr Phe Pro Ser Ser Asp
    130                 135                 140                 
Gly Ile Phe Trp Lys His Trp Ile Gln Thr Lys Asp Gly Gln Cys Gly
145                 150                 155                 160 
Ser Pro Leu Val Ser Thr Arg Asp Gly Phe Ile Val Gly Ile His Ser
                165                 170                 175     
Ala Ser Asn Phe Thr Asn Thr Asn Asn Tyr Phe Thr Ser Val Pro Lys
            180                 185                 190         
Asn Phe Met Glu Leu Leu Thr Asn Gln Glu Ala Gln Gln Trp Val Ser
        195                 200                 205             
Gly Trp Arg Leu Asn Ala Asp Ser Val Leu Trp Gly Gly His Lys Val
    210                 215                 220                 
Phe Met Asn Lys Pro Glu Glu Pro Phe Gln Pro Val Lys Glu Ala Thr
225                 230                 235                 240 
Gln Leu Met Asn Glu Leu Val Tyr Ser Gln
                245                 250 


<210> 71
<211> 753
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon-optimized TEV open reading frame

<400> 71
atgggtcacc accatcacca ccatggcgaa tctctgttca agggtccgcg tgattataac 60
ccgatatctt ctactatttg tcatctgact aacgaaagcg acggccacac gacttctctg 120
tacggtatcg gtttcggtcc gttcatcatt accgtgaagc atctgttccg ccgtaacaac 180
ggtaccctgg tggttcaatc tctgcacggc gtcttcaagg taaaaaatac cactacgctg 240
cagcagcacc tgattgacgg ccgtgacatg atcatcatcc gcatgccgaa agattttccg 300
ccgttcccgc aaaaactgaa gtttcgtgaa ccgcaacgcg aagaacgtat ttgcctggtt 360
accaccaact ttcagaccaa aagcatgtct tctatggttt ccgatacctc ttgcaccttc 420
ccaagcggtg acggtatttt ctggaaacat tggatccaga ccaaagatgg tcagtgcggc 480
tctccgctgg tgtctacgcg tgacggtttc atcgttggta tccattctgc ttctaacttc 540
actaacacta acaactactt tacttccgtt ccgaaaaact tcatggagct gctgactaac 600
caagaggccc agcagtgggt gtccggttgg cgcctgaacg cagattctgt actgtggggt 660
ggtcataagg tattcatgaa caaaccggag gagccgttcc agccggtcaa agaggcgacc 720
cagctgatga acgaactggt ttactctcag taa                              753

<210> 72
<211> 250
<212> PRT
<213> Artificial Sequence

<220> 
<223> TEV protease (N44V, L56V, S135G, S219N) with
      amino-terminus polyhistidine affinity tag

<400> 72
Met Gly His His His His His His Gly Glu Ser Leu Phe Lys Gly Pro
 1               5                  10                  15      
Arg Asp Tyr Asn Pro Ile Ser Ser Thr Ile Cys His Leu Thr Asn Glu
            20                  25                  30          
Ser Asp Gly His Thr Thr Ser Leu Tyr Gly Ile Gly Phe Gly Pro Phe
        35                  40                  45              
Ile Ile Thr Val Lys His Leu Phe Arg Arg Asn Asn Gly Thr Leu Val
    50                  55                  60                  
Val Gln Ser Leu His Gly Val Phe Lys Val Lys Asn Thr Thr Thr Leu
65                  70                  75                  80  
Gln Gln His Leu Ile Asp Gly Arg Asp Met Ile Ile Ile Arg Met Pro
                85                  90                  95      
Lys Asp Phe Pro Pro Phe Pro Gln Lys Leu Lys Phe Arg Glu Pro Gln
            100                 105                 110         
Arg Glu Glu Arg Ile Cys Leu Val Thr Thr Asn Phe Gln Thr Lys Ser
        115                 120                 125             
Met Ser Ser Met Val Ser Asp Thr Ser Cys Thr Phe Pro Ser Gly Asp
    130                 135                 140                 
Gly Ile Phe Trp Lys His Trp Ile Gln Thr Lys Asp Gly Gln Cys Gly
145                 150                 155                 160 
Ser Pro Leu Val Ser Thr Arg Asp Gly Phe Ile Val Gly Ile His Ser
                165                 170                 175     
Ala Ser Asn Phe Thr Asn Thr Asn Asn Tyr Phe Thr Ser Val Pro Lys
            180                 185                 190         
Asn Phe Met Glu Leu Leu Thr Asn Gln Glu Ala Gln Gln Trp Val Ser
        195                 200                 205             
Gly Trp Arg Leu Asn Ala Asp Ser Val Leu Trp Gly Gly His Lys Val
    210                 215                 220                 
Phe Met Asn Lys Pro Glu Glu Pro Phe Gln Pro Val Lys Glu Ala Thr
225                 230                 235                 240 
Gln Leu Met Asn Glu Leu Val Tyr Ser Gln
                245                 250 


<210> 73
<211> 753
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon-optimized TEV open reading frame

<400> 73
atgggtcacc accatcacca ccatggcgaa tctctgttca agggtccgcg tgattataac 60
ccgatatctt ctactatttg tcatctgact aacgaaagcg acggccacac gacttctctg 120
tacggtatcg gtttcggtcc gttcatcatt accaacaagc atctgttccg ccgtaacaac 180
ggtaccctgg tggttcaatc tctgcacggc gtcttcaagg taaaagacac cactacgctg 240
cagcagcacc tgattgacgg ccgtgacatg atcatcatcc gcatgccgaa agattttccg 300
ccgttcccgc aaaaactgaa gtttcgtgaa ccgcaacgcg aagaacgtat ttgcctggtt 360
accaccaact ttcagaccaa aagcatgtct tctatggttt ccgatacctc ttgcaccttc 420
ccaagcggtg acggtatttt ctggaaacat tggatccaga ccaaagatgg tcagtgcggc 480
tctccgctgg tgtctacgcg tgacggtttc atcgttggta tccattctgc ttctaacttc 540
actaacacta acaactactt tacttccgtt ccgaaaaact tcatggagct gctgactaac 600
caagaggccc agcagtgggt gtccggttgg cgcctgaacg cagattctgt actgtggggt 660
ggtcataagg tattcatgaa caaaccggag gagccgttcc agccggtcaa agaggcgacc 720
cagctgatga acgaactggt ttactctcag taa                              753

<210> 74
<211> 250
<212> PRT
<213> Artificial Sequence

<220> 
<223> TEV protease (L56V, N68D, S135G, S219N) with
      amino-terminus polyhistidine affinity tag

<400> 74
Met Gly His His His His His His Gly Glu Ser Leu Phe Lys Gly Pro
 1               5                  10                  15      
Arg Asp Tyr Asn Pro Ile Ser Ser Thr Ile Cys His Leu Thr Asn Glu
            20                  25                  30          
Ser Asp Gly His Thr Thr Ser Leu Tyr Gly Ile Gly Phe Gly Pro Phe
        35                  40                  45              
Ile Ile Thr Asn Lys His Leu Phe Arg Arg Asn Asn Gly Thr Leu Val
    50                  55                  60                  
Val Gln Ser Leu His Gly Val Phe Lys Val Lys Asp Thr Thr Thr Leu
65                  70                  75                  80  
Gln Gln His Leu Ile Asp Gly Arg Asp Met Ile Ile Ile Arg Met Pro
                85                  90                  95      
Lys Asp Phe Pro Pro Phe Pro Gln Lys Leu Lys Phe Arg Glu Pro Gln
            100                 105                 110         
Arg Glu Glu Arg Ile Cys Leu Val Thr Thr Asn Phe Gln Thr Lys Ser
        115                 120                 125             
Met Ser Ser Met Val Ser Asp Thr Ser Cys Thr Phe Pro Ser Gly Asp
    130                 135                 140                 
Gly Ile Phe Trp Lys His Trp Ile Gln Thr Lys Asp Gly Gln Cys Gly
145                 150                 155                 160 
Ser Pro Leu Val Ser Thr Arg Asp Gly Phe Ile Val Gly Ile His Ser
                165                 170                 175     
Ala Ser Asn Phe Thr Asn Thr Asn Asn Tyr Phe Thr Ser Val Pro Lys
            180                 185                 190         
Asn Phe Met Glu Leu Leu Thr Asn Gln Glu Ala Gln Gln Trp Val Ser
        195                 200                 205             
Gly Trp Arg Leu Asn Ala Asp Ser Val Leu Trp Gly Gly His Lys Val
    210                 215                 220                 
Phe Met Asn Lys Pro Glu Glu Pro Phe Gln Pro Val Lys Glu Ala Thr
225                 230                 235                 240 
Gln Leu Met Asn Glu Leu Val Tyr Ser Gln
                245                 250 


<210> 75
<211> 753
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon-optimized TEV open reading frame

<400> 75
atgggtcacc accatcacca ccatggcgaa tctctgttca agggtccgcg tgattataac 60
ccgatatctt cttctatttg tcatctgact aacgaaagcg acggccacac gacttctctg 120
tacggtatcg gtttcggtcc gttcatcatt accaacaagc atctgttccg ccgtaacaac 180
ggtaccctgg tggttcaatc tctgcacggc gtcttcaagg taaaagacac cactacgctg 240
cagcagcacc tggtcgacgg ccgtgacatg atcatcatcc gcatgccgaa agattttccg 300
ccgttcccgc aaaaactgaa gtttcgtgaa ccgcaacgcg aagaacgtat ttgcctggtt 360
accaccaact ttcagaccaa aagcatgtct tctatggttt ccgatacctc ttgcaccttc 420
ccaagctctg acggtatttt ctggaaacat tggatccaga ccaaagatgg tcagtgcggc 480
tctccgctgg tgtctacgcg tgacggtttc atcgttggta tccattctgc ttctaacttc 540
actaacacta acaactactt tacttccgtt ccgaaaaact tcatggagct gctgactaac 600
caagaggccc agcagtgggt gtccggttgg cgcctgaacg cagattctgt actgtggggt 660
ggtcataagg tattcatgaa caaaccggag gagccgttcc agccggtcaa agaggcgacc 720
cagctgatga acgaactggt ttactctcag taa                              753

<210> 76
<211> 250
<212> PRT
<213> Artificial Sequence

<220> 
<223> TEV protease (T17S, L56V, N68D, I77V, S219N) with
      amino-terminus polyhistidine affinity tag

<400> 76
Met Gly His His His His His His Gly Glu Ser Leu Phe Lys Gly Pro
 1               5                  10                  15      
Arg Asp Tyr Asn Pro Ile Ser Ser Ser Ile Cys His Leu Thr Asn Glu
            20                  25                  30          
Ser Asp Gly His Thr Thr Ser Leu Tyr Gly Ile Gly Phe Gly Pro Phe
        35                  40                  45              
Ile Ile Thr Asn Lys His Leu Phe Arg Arg Asn Asn Gly Thr Leu Val
    50                  55                  60                  
Val Gln Ser Leu His Gly Val Phe Lys Val Lys Asp Thr Thr Thr Leu
65                  70                  75                  80  
Gln Gln His Leu Val Asp Gly Arg Asp Met Ile Ile Ile Arg Met Pro
                85                  90                  95      
Lys Asp Phe Pro Pro Phe Pro Gln Lys Leu Lys Phe Arg Glu Pro Gln
            100                 105                 110         
Arg Glu Glu Arg Ile Cys Leu Val Thr Thr Asn Phe Gln Thr Lys Ser
        115                 120                 125             
Met Ser Ser Met Val Ser Asp Thr Ser Cys Thr Phe Pro Ser Ser Asp
    130                 135                 140                 
Gly Ile Phe Trp Lys His Trp Ile Gln Thr Lys Asp Gly Gln Cys Gly
145                 150                 155                 160 
Ser Pro Leu Val Ser Thr Arg Asp Gly Phe Ile Val Gly Ile His Ser
                165                 170                 175     
Ala Ser Asn Phe Thr Asn Thr Asn Asn Tyr Phe Thr Ser Val Pro Lys
            180                 185                 190         
Asn Phe Met Glu Leu Leu Thr Asn Gln Glu Ala Gln Gln Trp Val Ser
        195                 200                 205             
Gly Trp Arg Leu Asn Ala Asp Ser Val Leu Trp Gly Gly His Lys Val
    210                 215                 220                 
Phe Met Asn Lys Pro Glu Glu Pro Phe Gln Pro Val Lys Glu Ala Thr
225                 230                 235                 240 
Gln Leu Met Asn Glu Leu Val Tyr Ser Gln
                245                 250 


<210> 77
<211> 753
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon-optimized TEV open reading frame

<400> 77
atgggtcacc accatcacca ccatggcgaa tctctgttca agggtccgcg tgattataac 60
ccgatatctt cttctatttg tcatctgact aacgaaagcg acggccacac gacttctctg 120
tacggtatcg gtttcggtcc gttcatcatt accaacaagc atctgttccg ccgtaacaac 180
ggtaccctgc tggttcaatc tctgcacggc gtcttcaagg taaaagacac cactacgctg 240
cagcagcacc tggtcgacgg ccgtgacatg atcatcatcc gcatgccgaa agattttccg 300
ccgttcccgc aaaaactgaa gtttcgtgaa ccgcaacgcg aagaacgtat ttgcctggtt 360
accaccaact ttcagaccaa aagcatgtct tctatggttt ccgatacctc ttgcaccttc 420
ccaagcggtg acggtatttt ctggaaacat tggatccaga ccaaagatgg tcagtgcggc 480
tctccgctgg tgtctacgcg tgacggtttc atcgttggta tccattctgc ttctaacttc 540
actaacacta acaactactt tacttccgtt ccgaaaaact tcatggagct gctgactaac 600
caagaggccc agcagtgggt gtccggttgg cgcctgaacg cagattctgt actgtggggt 660
ggtcataagg tattcatgaa caaaccggag gagccgttcc agccggtcaa agaggcgacc 720
cagctgatga acgaactggt ttactctcag taa                              753

<210> 78
<211> 250
<212> PRT
<213> Artificial Sequence

<220> 
<223> TEV protease (T17S, N68D, I77V, S135G, S219N) with
      amino-terminus polyhistidine affinity tag

<400> 78
Met Gly His His His His His His Gly Glu Ser Leu Phe Lys Gly Pro
 1               5                  10                  15      
Arg Asp Tyr Asn Pro Ile Ser Ser Ser Ile Cys His Leu Thr Asn Glu
            20                  25                  30          
Ser Asp Gly His Thr Thr Ser Leu Tyr Gly Ile Gly Phe Gly Pro Phe
        35                  40                  45              
Ile Ile Thr Asn Lys His Leu Phe Arg Arg Asn Asn Gly Thr Leu Leu
    50                  55                  60                  
Val Gln Ser Leu His Gly Val Phe Lys Val Lys Asp Thr Thr Thr Leu
65                  70                  75                  80  
Gln Gln His Leu Val Asp Gly Arg Asp Met Ile Ile Ile Arg Met Pro
                85                  90                  95      
Lys Asp Phe Pro Pro Phe Pro Gln Lys Leu Lys Phe Arg Glu Pro Gln
            100                 105                 110         
Arg Glu Glu Arg Ile Cys Leu Val Thr Thr Asn Phe Gln Thr Lys Ser
        115                 120                 125             
Met Ser Ser Met Val Ser Asp Thr Ser Cys Thr Phe Pro Ser Gly Asp
    130                 135                 140                 
Gly Ile Phe Trp Lys His Trp Ile Gln Thr Lys Asp Gly Gln Cys Gly
145                 150                 155                 160 
Ser Pro Leu Val Ser Thr Arg Asp Gly Phe Ile Val Gly Ile His Ser
                165                 170                 175     
Ala Ser Asn Phe Thr Asn Thr Asn Asn Tyr Phe Thr Ser Val Pro Lys
            180                 185                 190         
Asn Phe Met Glu Leu Leu Thr Asn Gln Glu Ala Gln Gln Trp Val Ser
        195                 200                 205             
Gly Trp Arg Leu Asn Ala Asp Ser Val Leu Trp Gly Gly His Lys Val
    210                 215                 220                 
Phe Met Asn Lys Pro Glu Glu Pro Phe Gln Pro Val Lys Glu Ala Thr
225                 230                 235                 240 
Gln Leu Met Asn Glu Leu Val Tyr Ser Gln
                245                 250 


<210> 79
<211> 753
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon-optimized TEV open reading frame

<400> 79
atgggcgaat ctctgttcaa gggtccgcgt gattataacc cgatatcttc ttctatttgt 60
catctgacta acgaaagcga cggccacacg acttctctgt acggtatcgg tttcggtccg 120
ttcatcatta ccgtgaagca tctgttccgc cgtaacaacg gtaccctggt ggttcaatct 180
ctgcacggcg tcttcaaggt aaaagacacc actacgctgc agcagcacct ggtcgacggc 240
cgtgacatga tcatcatccg catgccgaaa gattttccgc cgttcccgca aaaactgaag 300
tttcgtgaac cgcaacgcga agaacgtatt tgcctggtta ccaccaactt tcagaccaaa 360
agcatgtctt ctatggtttc cgatacctct tgcaccttcc caagcggtga cggtattttc 420
tggaaacatt ggatccagac caaagatggt cagtgcggct ctccgctggt gtctacgcgt 480
gacggtttca tcgttggtat ccattctgct tctaacttca ctaacactaa caactacttt 540
acttccgttc cgaaaaactt catggagctg ctgactaacc aagaggccca gcagtgggtg 600
tccggttggc gcctgaacgc agattctgta ctgtggggtg gtcataaggt attcatgaac 660
aaaccggagg agccgttcca gccggtcaaa gaggcgaccc agctgatgaa cgaactggtt 720
tactctcagg gtcaccacca tcaccaccat taa                              753

<210> 80
<211> 250
<212> PRT
<213> Artificial Sequence

<220> 
<223> TEV protease (T17S, N44V, L56V, N68D, I77V, S135G,
      S219N) with carboxyl-terminus polyhistidine
      affinity tag

<400> 80
Met Gly Glu Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser
 1               5                  10                  15      
Ser Ser Ile Cys His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser
            20                  25                  30          
Leu Tyr Gly Ile Gly Phe Gly Pro Phe Ile Ile Thr Val Lys His Leu
        35                  40                  45              
Phe Arg Arg Asn Asn Gly Thr Leu Val Val Gln Ser Leu His Gly Val
    50                  55                  60                  
Phe Lys Val Lys Asp Thr Thr Thr Leu Gln Gln His Leu Val Asp Gly
65                  70                  75                  80  
Arg Asp Met Ile Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro
                85                  90                  95      
Gln Lys Leu Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu
            100                 105                 110         
Val Thr Thr Asn Phe Gln Thr Lys Ser Met Ser Ser Met Val Ser Asp
        115                 120                 125             
Thr Ser Cys Thr Phe Pro Ser Gly Asp Gly Ile Phe Trp Lys His Trp
    130                 135                 140                 
Ile Gln Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg
145                 150                 155                 160 
Asp Gly Phe Ile Val Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr
                165                 170                 175     
Asn Asn Tyr Phe Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr
            180                 185                 190         
Asn Gln Glu Ala Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp
        195                 200                 205             
Ser Val Leu Trp Gly Gly His Lys Val Phe Met Asn Lys Pro Glu Glu
    210                 215                 220                 
Pro Phe Gln Pro Val Lys Glu Ala Thr Gln Leu Met Asn Glu Leu Val
225                 230                 235                 240 
Tyr Ser Gln Gly His His His His His His
                245                 250 


<210> 81
<211> 753
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon-optimized TEV open reading frame

<400> 81
atgggtcacc accatcacca ccatggcgaa tctctgttca agggtccgcg tgattataac 60
ccgatatctt cttctatttg tcatctgact aacgaaagcg acggccacac gacttctctg 120
tacggtatcg gtttcggtcc gttcatcatt accgtgaagc atctgttccg ccgtaacaac 180
ggtaccctgg tggttcaatc tctgcacggc gtcttcaagg taaaagacac cactacgctg 240
cagcagcacc tggtcgacgg ccgtgacatg atcatcatcc gcatgccgaa agattttccg 300
ccgttcccgc aaaaactgaa gtttcgtgaa ccgcaacgcg aagaacgtat ttgcctggtt 360
accaccaact ttcagaccaa aagcatgtct tctatggttt ccgatacctc ttgcaccttc 420
ccaagcggtg acggtatttt ctggaaacat tggatccaga ccaaagatgg tcagtgcggc 480
tctccgctgg tgtctacgcg tgacggtttc atcgttggta tccattctgc ttctaacttc 540
actaacacta acaactactt tacttccgtt ccgaaaaact tcatggagct gctgactaac 600
caagaggccc agcagtgggt gtccggttgg cgcctgaacg cagattctgt actgtggggt 660
ggtcataagg tattcatggt gaaaccggag gagccgttcc agccggtcaa agaggcgacc 720
cagctgatga acgaactggt ttactctcag taa                              753

<210> 82
<211> 250
<212> PRT
<213> Artificial Sequence

<220> 
<223> TEV protease (T17S, N44V, L56V, N68D, I77V, S135G,
      S219V) with amino-terminus polyhistidine affinity
      tag

<400> 82
Met Gly His His His His His His Gly Glu Ser Leu Phe Lys Gly Pro
 1               5                  10                  15      
Arg Asp Tyr Asn Pro Ile Ser Ser Ser Ile Cys His Leu Thr Asn Glu
            20                  25                  30          
Ser Asp Gly His Thr Thr Ser Leu Tyr Gly Ile Gly Phe Gly Pro Phe
        35                  40                  45              
Ile Ile Thr Val Lys His Leu Phe Arg Arg Asn Asn Gly Thr Leu Val
    50                  55                  60                  
Val Gln Ser Leu His Gly Val Phe Lys Val Lys Asp Thr Thr Thr Leu
65                  70                  75                  80  
Gln Gln His Leu Val Asp Gly Arg Asp Met Ile Ile Ile Arg Met Pro
                85                  90                  95      
Lys Asp Phe Pro Pro Phe Pro Gln Lys Leu Lys Phe Arg Glu Pro Gln
            100                 105                 110         
Arg Glu Glu Arg Ile Cys Leu Val Thr Thr Asn Phe Gln Thr Lys Ser
        115                 120                 125             
Met Ser Ser Met Val Ser Asp Thr Ser Cys Thr Phe Pro Ser Gly Asp
    130                 135                 140                 
Gly Ile Phe Trp Lys His Trp Ile Gln Thr Lys Asp Gly Gln Cys Gly
145                 150                 155                 160 
Ser Pro Leu Val Ser Thr Arg Asp Gly Phe Ile Val Gly Ile His Ser
                165                 170                 175     
Ala Ser Asn Phe Thr Asn Thr Asn Asn Tyr Phe Thr Ser Val Pro Lys
            180                 185                 190         
Asn Phe Met Glu Leu Leu Thr Asn Gln Glu Ala Gln Gln Trp Val Ser
        195                 200                 205             
Gly Trp Arg Leu Asn Ala Asp Ser Val Leu Trp Gly Gly His Lys Val
    210                 215                 220                 
Phe Met Val Lys Pro Glu Glu Pro Phe Gln Pro Val Lys Glu Ala Thr
225                 230                 235                 240 
Gln Leu Met Asn Glu Leu Val Tyr Ser Gln
                245                 250 


<210> 83
<211> 753
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon-optimized TEV open reading frame

<400> 83
atgggtcacc accatcacca ccatggcgaa tctctgttca agggtccgcg tgattataac 60
ccgatatctt cttctatttg tcatctgact aacgaaagcg acggccacac gacttctctg 120
tacggtatcg gtttcggtcc gttcatcatt accgtgaagc atctgttccg ccgtaacaac 180
ggtaccctgg tggttcaatc tctgcacggc gtcttcaagg taaaagacac cactacgctg 240
cagcagcacc tggtcgacgg ccgtgacatg atcatcatcc gcatgccgaa agattttccg 300
ccgttcccgc aaaaactgaa gtttcgtgaa ccgcaacgcg aagaacgtat ttgcctggtt 360
accaccaact ttcagaccaa aagcatgtct tctatggttt ccgatacctc ttgcaccttc 420
ccaagcggtg acggtatttt ctggaaacat tggatccaga ccaaagatgg tcagtgcggc 480
tctccgctgg tgtctacgcg tgacggtttc atcgttggta tccattctgc ttctaacttc 540
actaacacta acaactactt tacttccgtt ccgaaaaact tcatggagct gctgactaac 600
caagaggccc agcagtgggt gtccggttgg cgcctgaacg cagattctgt actgtggggt 660
ggtcataagg tattcatgaa caaaccggag gagccgttcc agccggtcaa agaggcgacc 720
cagctgatga acgaactggt ttactctcag taa                              753

<210> 84
<211> 250
<212> PRT
<213> Artificial Sequence

<220> 
<223> TEV protease (T17S, N44V, L56V, N68D, I77V, S135G,
      S219N) with amino-terminus polyhistidine affinity
      tag

<400> 84
Met Gly His His His His His His Gly Glu Ser Leu Phe Lys Gly Pro
 1               5                  10                  15      
Arg Asp Tyr Asn Pro Ile Ser Ser Ser Ile Cys His Leu Thr Asn Glu
            20                  25                  30          
Ser Asp Gly His Thr Thr Ser Leu Tyr Gly Ile Gly Phe Gly Pro Phe
        35                  40                  45              
Ile Ile Thr Val Lys His Leu Phe Arg Arg Asn Asn Gly Thr Leu Val
    50                  55                  60                  
Val Gln Ser Leu His Gly Val Phe Lys Val Lys Asp Thr Thr Thr Leu
65                  70                  75                  80  
Gln Gln His Leu Val Asp Gly Arg Asp Met Ile Ile Ile Arg Met Pro
                85                  90                  95      
Lys Asp Phe Pro Pro Phe Pro Gln Lys Leu Lys Phe Arg Glu Pro Gln
            100                 105                 110         
Arg Glu Glu Arg Ile Cys Leu Val Thr Thr Asn Phe Gln Thr Lys Ser
        115                 120                 125             
Met Ser Ser Met Val Ser Asp Thr Ser Cys Thr Phe Pro Ser Gly Asp
    130                 135                 140                 
Gly Ile Phe Trp Lys His Trp Ile Gln Thr Lys Asp Gly Gln Cys Gly
145                 150                 155                 160 
Ser Pro Leu Val Ser Thr Arg Asp Gly Phe Ile Val Gly Ile His Ser
                165                 170                 175     
Ala Ser Asn Phe Thr Asn Thr Asn Asn Tyr Phe Thr Ser Val Pro Lys
            180                 185                 190         
Asn Phe Met Glu Leu Leu Thr Asn Gln Glu Ala Gln Gln Trp Val Ser
        195                 200                 205             
Gly Trp Arg Leu Asn Ala Asp Ser Val Leu Trp Gly Gly His Lys Val
    210                 215                 220                 
Phe Met Asn Lys Pro Glu Glu Pro Phe Gln Pro Val Lys Glu Ala Thr
225                 230                 235                 240 
Gln Leu Met Asn Glu Leu Val Tyr Ser Gln
                245                 250 


<210> 85
<211> 750
<212> DNA
<213> Artificial Sequence

<220> 
<223> Native TEV open reading frame

<400> 85
atgcatcacc atcaccacca tggagaaagc ttgtttaagg gaccacgtga ttacaacccg 60
atatcgagca ccatttgtca tttgacgaat gaatctgatg ggcacacaac atcgttgtat 120
ggtattggat ttggtccctt catcattaca aacaagcact tgtttcgccg taataatgga 180
acactgttgg tccaatcact acatggtgta ttcaaggtca agaacaccac gactttgcaa 240
caacacctca ttgatgggag ggacatgata attattcgca tgcctaagga tttcccacca 300
tttcctcaaa agctgaaatt tagagagcca caaagggaag agcgcatctg tcttgtgaca 360
accaacttcc aaactaagag catgtctagc atggtgtcag acactagttg cacattccct 420
tcatctgatg gcatattctg gaagcattgg atccaaacca aggatgggca gtgtggcagt 480
ccattagtat caactagaga tgggttcatt gttggtatac actcagcatc gaatttcacc 540
aacacaaaca attatttcac aagcgtgccg aaaaacttca tggaattgtt gacaaatcag 600
gaggcgcagc agtgggttag tggttggcga ttaaatgctg actcagtatt gtgggggggc 660
cataaagttt tcatgaacaa acctgaagag ccttttcagc cagttaagga agcgactcaa 720
ctcatgaatg aattggtgta ctcgcaataa                                  750

<210> 86
<211> 249
<212> PRT
<213> Artificial Sequence

<220> 
<223> TEV protease (S219N) with amino-terminus
      polyhistidine affinity tag

<400> 86
Met His His His His His His Gly Glu Ser Leu Phe Lys Gly Pro Arg
 1               5                  10                  15      
Asp Tyr Asn Pro Ile Ser Ser Thr Ile Cys His Leu Thr Asn Glu Ser
            20                  25                  30          
Asp Gly His Thr Thr Ser Leu Tyr Gly Ile Gly Phe Gly Pro Phe Ile
        35                  40                  45              
Ile Thr Asn Lys His Leu Phe Arg Arg Asn Asn Gly Thr Leu Leu Val
    50                  55                  60                  
Gln Ser Leu His Gly Val Phe Lys Val Lys Asn Thr Thr Thr Leu Gln
65                  70                  75                  80  
Gln His Leu Ile Asp Gly Arg Asp Met Ile Ile Ile Arg Met Pro Lys
                85                  90                  95      
Asp Phe Pro Pro Phe Pro Gln Lys Leu Lys Phe Arg Glu Pro Gln Arg
            100                 105                 110         
Glu Glu Arg Ile Cys Leu Val Thr Thr Asn Phe Gln Thr Lys Ser Met
        115                 120                 125             
Ser Ser Met Val Ser Asp Thr Ser Cys Thr Phe Pro Ser Ser Asp Gly
    130                 135                 140                 
Ile Phe Trp Lys His Trp Ile Gln Thr Lys Asp Gly Gln Cys Gly Ser
145                 150                 155                 160 
Pro Leu Val Ser Thr Arg Asp Gly Phe Ile Val Gly Ile His Ser Ala
                165                 170                 175     
Ser Asn Phe Thr Asn Thr Asn Asn Tyr Phe Thr Ser Val Pro Lys Asn
            180                 185                 190         
Phe Met Glu Leu Leu Thr Asn Gln Glu Ala Gln Gln Trp Val Ser Gly
        195                 200                 205             
Trp Arg Leu Asn Ala Asp Ser Val Leu Trp Gly Gly His Lys Val Phe
    210                 215                 220                 
Met Asn Lys Pro Glu Glu Pro Phe Gln Pro Val Lys Glu Ala Thr Gln
225                 230                 235                 240 
Leu Met Asn Glu Leu Val Tyr Ser Gln
                245                 


<210> 87
<211> 3984
<212> DNA
<213> Artificial Sequence

<220> 
<223> Open reading frame encoding BoNT/A-TEV

<400> 87
atgccgttcg taaacaaaca gttcaactat aaagacccag tcaacggcgt ggacattgcc 60
tatatcaaaa tcccgaatgc gggtcaaatg cagcccgtga aagcatttaa aatccataac 120
aaaatttggg tgatcccgga gcgcgatacg ttcacgaacc cggaagaagg agatttaaac 180
ccaccgcctg aggctaaaca ggtcccggtg tcttactatg atagcacata cctgagtacc 240
gacaatgaaa aggacaacta cctgaaaggt gttaccaaac tgttcgagcg catttattcg 300
acagatctcg gtcgcatgtt gctgacttct attgtgcgcg gcattccgtt ttggggtggt 360
agcaccatcg atacagaact caaagtgatt gacaccaact gcatcaatgt gattcagcct 420
gatgggagct accggtccga agagcttaac ctcgtaatca ttggcccgag cgcggatatt 480
atccaattcg aatgtaaatc ttttgggcat gaagtcctga atctgacgcg gaatggctat 540
ggatcgacgc agtatattcg tttttctcca gatttcacat ttggatttga agaaagcctc 600
gaagttgata cgaaccctct tttaggcgcg ggaaaattcg cgacggaccc agcggtgacc 660
ttggcacatg aacttattca tgccgggcat cgcttgtatg gaatcgccat taacccgaac 720
cgtgttttca aggtgaatac gaacgcgtat tacgagatgt cgggcttaga agtgtccttt 780
gaagaactgc gcacgtttgg cggtcatgat gcaaaattta ttgatagtct gcaagaaaac 840
gaatttcggc tgtactatta caataaattc aaagacattg catcaacctt aaacaaggcg 900
aaaagcattg tgggtaccac ggctagctta caatatatga aaaacgtttt caaagaaaaa 960
tacctcctta gcgaagacac ttccggcaaa ttctctgtcg ataaactgaa atttgataaa 1020
ctgtataaaa tgctcaccga gatctacaca gaggataact ttgtcaaatt cttcaaggtc 1080
ttgaatcgga aaacctatct gaacttcgat aaagccgtct ttaagatcaa catcgtaccg 1140
aaagttaact acaccatcta tgatggcttt aatctgcgca atacgaatct ggcggcgaac 1200
tttaacggcc agaacaccga aatcaacaac atgaacttta ctaaactgaa aaattttacc 1260
ggcttgtttg aattctataa gctcctgtgt gtccgcggta ttatcaccag caaaaccaaa 1320
tccttgggcg gtggtggcga aaacctgtac ttccagggcg gtggcggtgg tgataagggc 1380
tataacaagg ccttaaatga tttatgcatc aaggtgaaca actgggactt gtttttctct 1440
ccatctgaag ataattttac taacgacttg aacaaaggag aggaaattac ttccgatacc 1500
aacatcgaag cagcggaaga gaatattagc ctggatctta ttcaacaata ttacctgacc 1560
tttaattttg ataacgagcc tgagaacatt tccattgaga atctcagctc tgacatcatc 1620
ggccagctgg aactgatgcc gaatatcgaa cgctttccta atggaaagaa atatgaattg 1680
gacaaataca ccatgttcca ctatctccgc gcgcaggagt ttgagcacgg caagtctcgt 1740
attgctctga ccaattcggt aaacgaagcc cttttaaatc cttcgcgtgt gtacaccttt 1800
ttctcaagcg attatgttaa aaaagtgaac aaggcgaccg aagcggcgat gtttttggga 1860
tgggtggaac aactggtata tgactttacg gatgaaactt ctgaagtctc gaccaccgac 1920
aaaattgccg atattaccat tatcattccc tatattggcc ctgcactgaa cattggtaac 1980
atgctgtata aagatgattt tgtgggcgcc ctgatctttt caggcgctgt tatcctgctg 2040
gaatttatcc cggaaatcgc cattccagta ctcggtacct ttgcgctggt gtcctatatc 2100
gcaaacaaag ttttgactgt ccagacgatc gacaacgcgc tcagtaaacg taacgaaaaa 2160
tgggatgagg tgtataagta tattgttacc aactggctcg ctaaagtaaa cacccagatt 2220
gacctgattc gcaagaagat gaaagaagcg ctggaaaacc aagcagaagc gaccaaagct 2280
attatcaact atcaatataa ccagtacaca gaggaagaaa agaataacat caacttcaac 2340
atcgacgact tatcttcaaa gctgaatgaa tctattaaca aagcgatgat taatattaac 2400
aagttcttga accaatgtag tgtcagctat ctgatgaact cgatgatccc ttacggtgtg 2460
aaacgtctgg aagacttcga tgcaagcctt aaagatgccc ttctgaagta tatttacgat 2520
aatcgcggaa ctcttattgg ccaagtggat cgcttaaaag ataaagtcaa caacacgctg 2580
agtacagaca tcccttttca gctgtctaaa tatgtggaca atcagcgcct gctgtccacg 2640
tttacggaat acatcaaaaa catcatcaac actagtattc tgaacttgcg ttacgagagt 2700
aaccatctga ttgatctgag ccgttacgca tctaaaatca acatcggctc gaaggtgaac 2760
ttcgatccta tcgacaaaaa ccagattcaa ttgttcaact tagaatcgtc aaagattgaa 2820
gttatcttaa aaaatgcgat tgtatataat tcaatgtacg aaaatttctc tacgagcttt 2880
tggattcgta ttccgaaata tttcaacagt atctctttaa acaacgagta tactatcatc 2940
aattgtatgg agaataacag cgggtggaaa gtgagcctta actatggtga aatcatctgg 3000
actctgcagg acactcaaga aattaaacaa cgcgtggtgt ttaaatactc acagatgatt 3060
aacatctcgg attatattaa tcgctggatt tttgtgacaa ttactaacaa ccggctgaac 3120
aacagcaaaa tttacattaa cggtcgcctg atcgatcaga aaccaatcag taatctcggt 3180
aacattcacg catcgaataa tatcatgttc aaactggatg gttgtcgcga cacgcaccgt 3240
tacatttgga tcaaatactt caatttattc gacaaagaac tcaacgaaaa ggagattaag 3300
gatctttatg acaatcagtc taattcgggt attctgaaag acttttgggg tgattacctt 3360
cagtacgata aaccgtatta tatgttaaac ttatatgatc cgaataaata cgttgacgtc 3420
aacaacgttg gcattcgtgg ctatatgtat ctgaaagggc cgcgtggcag cgtgatgacc 3480
actaacattt acttaaactc ctccctctat cgcggtacta aatttattat caagaaatat 3540
gcctctggca acaaggacaa tatcgtacgc aataacgatc gcgtctacat taacgtggtg 3600
gtgaagaata aagaatatcg tctggcgacc aatgctagtc aggcgggcgt ggagaaaatt 3660
ctgtctgcac ttgaaatccc ggatgtgggt aatttatccc aggtggttgt gatgaaaagt 3720
aaaaatgacc aagggatcac caataaatgc aaaatgaatc tgcaagataa caacggcaac 3780
gacattggtt ttatcggctt ccaccaattc aataatatcg cgaaactggt ggcctcaaat 3840
tggtacaacc gtcagattga gcgcagctcc cgcactttag gctgtagctg ggagttcatt 3900
ccggtagatg acggttgggg agaacgccca ttgaaagtcg acaagcttgc ggccgcactc 3960
gagcaccacc accaccacca ctga                                        3984

<210> 88
<211> 1327
<212> PRT
<213> Artificial Sequence

<220> 
<223> BoNT/A-TEV

<400> 88
Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly
 1               5                  10                  15      
Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro
            20                  25                  30          
Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg
        35                  40                  45              
Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu
    50                  55                  60                  
Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr
65                  70                  75                  80  
Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu
                85                  90                  95      
Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val
            100                 105                 110         
Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys
        115                 120                 125             
Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr
    130                 135                 140                 
Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile
145                 150                 155                 160 
Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr
                165                 170                 175     
Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe
            180                 185                 190         
Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu
        195                 200                 205             
Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu
    210                 215                 220                 
Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn
225                 230                 235                 240 
Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu
                245                 250                 255     
Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys
            260                 265                 270         
Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn
        275                 280                 285             
Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val
    290                 295                 300                 
Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys
305                 310                 315                 320 
Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu
                325                 330                 335     
Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp
            340                 345                 350         
Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn
        355                 360                 365             
Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr
    370                 375                 380                 
Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn
385                 390                 395                 400 
Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu
                405                 410                 415     
Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg
            420                 425                 430         
Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Gly Gly Gly Gly Glu Asn
        435                 440                 445             
Leu Tyr Phe Gln Gly Gly Gly Gly Gly Asp Lys Gly Tyr Asn Lys Ala
    450                 455                 460                 
Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe Ser
465                 470                 475                 480 
Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu Ile
                485                 490                 495     
Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu Asp
            500                 505                 510         
Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro Glu
        515                 520                 525             
Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu Glu
    530                 535                 540                 
Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu Leu
545                 550                 555                 560 
Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu His
                565                 570                 575     
Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu Leu
            580                 585                 590         
Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys Lys
        595                 600                 605             
Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu Gln
    610                 615                 620                 
Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr Asp
625                 630                 635                 640 
Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala Leu
                645                 650                 655     
Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu Ile
            660                 665                 670         
Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala Ile
        675                 680                 685             
Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys Val
    690                 695                 700                 
Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu Lys
705                 710                 715                 720 
Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys Val
                725                 730                 735     
Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu Glu
            740                 745                 750         
Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn Gln
        755                 760                 765             
Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp Leu
    770                 775                 780                 
Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile Asn
785                 790                 795                 800 
Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met Ile
                805                 810                 815     
Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys Asp
            820                 825                 830         
Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly Gln
        835                 840                 845             
Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp Ile
    850                 855                 860                 
Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser Thr
865                 870                 875                 880 
Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn Leu
                885                 890                 895     
Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser Lys
            900                 905                 910         
Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn Gln
        915                 920                 925             
Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu Lys
    930                 935                 940                 
Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser Phe
945                 950                 955                 960 
Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn Glu
                965                 970                 975     
Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val Ser
            980                 985                 990         
Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu Ile
        995                 1000                1005            
Lys Gln Arg Val Val Phe Lys Tyr Ser Gln Met Ile Asn Ile Ser Asp
    1010                1015                1020                
Tyr Ile Asn Arg Trp Ile Phe Val Thr Ile Thr Asn Asn Arg Leu Asn
1025                1030                1035                1040
Asn Ser Lys Ile Tyr Ile Asn Gly Arg Leu Ile Asp Gln Lys Pro Ile
                1045                1050                1055    
Ser Asn Leu Gly Asn Ile His Ala Ser Asn Asn Ile Met Phe Lys Leu
            1060                1065                1070        
Asp Gly Cys Arg Asp Thr His Arg Tyr Ile Trp Ile Lys Tyr Phe Asn
        1075                1080                1085            
Leu Phe Asp Lys Glu Leu Asn Glu Lys Glu Ile Lys Asp Leu Tyr Asp
    1090                1095                1100                
Asn Gln Ser Asn Ser Gly Ile Leu Lys Asp Phe Trp Gly Asp Tyr Leu
1105                1110                1115                1120
Gln Tyr Asp Lys Pro Tyr Tyr Met Leu Asn Leu Tyr Asp Pro Asn Lys
                1125                1130                1135    
Tyr Val Asp Val Asn Asn Val Gly Ile Arg Gly Tyr Met Tyr Leu Lys
            1140                1145                1150        
Gly Pro Arg Gly Ser Val Met Thr Thr Asn Ile Tyr Leu Asn Ser Ser
        1155                1160                1165            
Leu Tyr Arg Gly Thr Lys Phe Ile Ile Lys Lys Tyr Ala Ser Gly Asn
    1170                1175                1180                
Lys Asp Asn Ile Val Arg Asn Asn Asp Arg Val Tyr Ile Asn Val Val
1185                1190                1195                1200
Val Lys Asn Lys Glu Tyr Arg Leu Ala Thr Asn Ala Ser Gln Ala Gly
                1205                1210                1215    
Val Glu Lys Ile Leu Ser Ala Leu Glu Ile Pro Asp Val Gly Asn Leu
            1220                1225                1230        
Ser Gln Val Val Val Met Lys Ser Lys Asn Asp Gln Gly Ile Thr Asn
        1235                1240                1245            
Lys Cys Lys Met Asn Leu Gln Asp Asn Asn Gly Asn Asp Ile Gly Phe
    1250                1255                1260                
Ile Gly Phe His Gln Phe Asn Asn Ile Ala Lys Leu Val Ala Ser Asn
1265                1270                1275                1280
Trp Tyr Asn Arg Gln Ile Glu Arg Ser Ser Arg Thr Leu Gly Cys Ser
                1285                1290                1295    
Trp Glu Phe Ile Pro Val Asp Asp Gly Trp Gly Glu Arg Pro Leu Lys
            1300                1305                1310        
Val Asp Lys Leu Ala Ala Ala Leu Glu His His His His His His
        1315                1320                1325        


<210> 89
<211> 1044
<212> DNA
<213> Artificial Sequence

<220> 
<223> Intervening sequence containing transcriptional
      and translational sites.

<400> 89
aagcttgtgg cctcaaattg gtacaaccgt cagattgagc gcagctcccg cactttaggc 60
tgtagctggg agttcattcc ggtagatgac ggttggggag aacgcccatt gcaccatcat 120
caccatcact gagcggccgc ataatgctta agtcgaacag attgatatgt agctataagt 180
aatcgtattg tacacggccg cataatcgaa attaatacga ctcactatag gggaattgtg 240
agcggataac aattccccat cttagtatat tagttaagta taagaaggag atataccatg 300
ggcgaatctc tgttcaaggg tccgcgtgat tataacccga tatcttcttc tatttgtcat 360
ctgactaacg aaagcgacgg ccacacgact tctctgtacg gtatcggttt cggtccgttc 420
atcattacca acaagcatct gttccgccgt aacaacggta ccctgctggt tcaatctctg 480
cacggcgtct tcaaggtaaa agacaccact acgctgcagc agcacctggt cgacggccgt 540
gacatgatca tcatccgcat gccgaaagat tttccgccgt tcccgcaaaa actgaagttt 600
cgtgaaccgc aacgcgaaga acgtatttgc ctggttacca ccaactttca gaccaaaagc 660
atgtcttcta tggtttccga tacctcttgc accttcccaa gcggtgacgg tattttctgg 720
aaacattgga ttcagaccaa agatggtcag tgcggctctc cgctggtgtc tacgcgtgac 780
ggtttcatcg ttggtatcca ttctgcttct aacttcacta acactaacaa ctactttact 840
tccgttccga aaaacttcat ggagctgctg actaaccaag aggcccagca gtgggtgtcc 900
ggttggcgcc tgaacgcaga ttctgtactg tggggtggtc ataaggtatt catgaacaaa 960
ccggaggagc cgttccagcc ggtcaaagag gcgacccagc tgatgaacga actggtttac 1020
tctcagtaag agctctgtct cgag                                        1044

<210> 90
<211> 4851
<212> DNA
<213> Artificial Sequence

<220> 
<223> BoNT/A-TEV and TEV protease variant 4 open reading
      frames with the intervening transcription and
      translation elements.

<400> 90
atgccgttcg taaacaaaca gttcaactat aaagacccag tcaacggcgt ggacattgcc 60
tatatcaaaa tcccgaatgc gggtcaaatg cagcccgtga aagcatttaa aatccataac 120
aaaatttggg tgatcccgga gcgcgatacg ttcacgaacc cggaagaagg agatttaaac 180
ccaccgcctg aggctaaaca ggtcccggtg tcttactatg atagcacata cctgagtacc 240
gacaatgaaa aggacaacta cctgaaaggt gttaccaaac tgttcgagcg catttattcg 300
acagatctcg gtcgcatgtt gctgacttct attgtgcgcg gcattccgtt ttggggtggt 360
agcaccatcg atacagaact caaagtgatt gacaccaact gcatcaatgt gattcagcct 420
gatgggagct accggtccga agagcttaac ctcgtaatca ttggcccgag cgcggatatt 480
atccaattcg aatgtaaatc ttttgggcat gaagtcctga atctgacgcg gaatggctat 540
ggatcgacgc agtatattcg tttttctcca gatttcacat ttggatttga agaaagcctc 600
gaagttgata cgaaccctct tttaggcgcg ggaaaattcg cgacggaccc agcggtgacc 660
ttggcacatg aacttattca tgccgggcat cgcttgtatg gaatcgccat taacccgaac 720
cgtgttttca aggtgaatac gaacgcgtat tacgagatgt cgggcttaga agtgtccttt 780
gaagaactgc gcacgtttgg cggtcatgat gcaaaattta ttgatagtct gcaagaaaac 840
gaatttcggc tgtactatta caataaattc aaagacattg catcaacctt aaacaaggcg 900
aaaagcattg tgggtaccac ggctagctta caatatatga aaaacgtttt caaagaaaaa 960
tacctcctta gcgaagacac ttccggcaaa ttctctgtcg ataaactgaa atttgataaa 1020
ctgtataaaa tgctcaccga gatctacaca gaggataact ttgtcaaatt cttcaaggtc 1080
ttgaatcgga aaacctatct gaacttcgat aaagccgtct ttaagatcaa catcgtaccg 1140
aaagttaact acaccatcta tgatggcttt aatctgcgca atacgaatct ggcggcgaac 1200
tttaacggcc agaacaccga aatcaacaac atgaacttta ctaaactgaa aaattttacc 1260
ggcttgtttg aattctataa gctcctgtgt gtccgcggta ttatcaccag caaaaccaaa 1320
tccttgggcg gtggtggcga aaacctgtac ttccagggcg gtggcggtgg tgataagggc 1380
tataacaagg ccttcaatga tttatgcatc aaggtgaaca actgggactt gtttttctct 1440
ccatctgaag ataattttac taacgacttg aacaaaggag aggaaattac ttccgatacc 1500
aacatcgaag cagcggaaga gaatattagt ctagatctta ttcaacaata ttacctgacc 1560
tttaattttg ataacgagcc tgagaacatt tccattgaga atctcagctc tgacatcatc 1620
ggccagctgg aactgatgcc gaatatcgaa cgctttccta atggaaagaa atatgaattg 1680
gacaaataca ccatgttcca ctatctccgc gcgcaggagt ttgagcacgg caagtctcgt 1740
attgctctga ccaattcggt aaacgaagcc cttttaaatc cttcgcgtgt gtacaccttt 1800
ttctcaagcg attatgttaa aaaagtgaac aaggcgaccg aagcggcgat gtttttggga 1860
tgggtggaac aactggtata tgactttacg gatgaaactt ctgaagtctc gaccaccgac 1920
aaaattgccg atattaccat tatcattccc tatattggcc ctgcactgaa cattggtaac 1980
atgctgtata aagatgattt tgtgggcgcc ctgatctttt caggcgctgt tatcctgctg 2040
gaatttatcc cggaaatcgc cattccagta ctcggtacct ttgcgctggt gtcctatatc 2100
gcaaacaaag ttttgactgt ccagacgatc gacaacgcgc tcagtaaacg taacgaaaaa 2160
tgggatgagg tgtataagta tattgttacc aactggctcg ctaaagtaaa cacccagatt 2220
gacctgattc gcaagaagat gaaagaagcg ctggaaaacc aagcagaagc gaccaaagct 2280
attatcaact atcaatataa ccagtacaca gaggaagaaa agaataacat caacttcaac 2340
atcgacgact tatcttcaaa gctgaatgaa tctattaaca aagcgatgat taatattaac 2400
aagttcttga accaatgtag tgtcagctat ctgatgaact cgatgatccc ttacggtgtg 2460
aaacgtctgg aagacttcga tgcaagcctt aaagatgccc ttctgaagta tatttacgat 2520
aatcgcggaa ctcttattgg ccaagtggat cgcttaaaag ataaagtcaa caacacgctg 2580
agtacagaca tcccttttca gctgtctaaa tatgtggaca atcagcgcct gctgtccacg 2640
tttacggaat acatcaaaaa catcatcaac actagtattc tgaacttgcg ttacgagagt 2700
aaccatctga ttgatctgag ccgttacgca tctaaaatca acatcggatc caaggtgaac 2760
ttcgatccta tcgacaaaaa ccagattcaa ttgttcaact tagaatcgtc aaagattgaa 2820
gttatcttaa aaaatgcgat tgtatataat tcaatgtacg aaaatttctc tacgagcttt 2880
tggattcgta ttccgaaata tttcaacagt atctctttaa acaacgagta tactatcatc 2940
aattgtatgg agaataacag cgggtggaaa gtgagcctta actatggtga aatcatctgg 3000
actctgcagg acactcaaga aattaaacaa cgcgtggtgt ttaaatactc acagatgatt 3060
aacatctcgg attatattaa tcgctggatt tttgtgacaa ttactaacaa ccggctgaac 3120
aacagcaaaa tttacattaa cggtcgcctg atcgatcaga aaccaatcag taatctcggt 3180
aacattcacg catcgaataa tatcatgttc aaactggatg gttgtcgcga cacgcaccgt 3240
tacatttgga tcaaatactt caatttattc gacaaagaac tcaacgaaaa ggagattaag 3300
gatctttatg acaatcagtc taattcgggt attctgaaag acttttgggg tgattacctt 3360
cagtacgata aaccgtatta tatgttaaac ttatatgatc cgaataaata cgttgacgtc 3420
aacaacgttg gcattcgtgg ctatatgtat ctgaaagggc cgcgtggcag cgtgatgacc 3480
actaacattt acttaaactc ctccctctat cgcggtacta aatttattat caagaaatat 3540
gcctctggca acaaggacaa tatcgtacgc aataacgatc gcgtctacat taacgtggtg 3600
gtgaagaata aagaatatcg tctggcgacc aatgctagtc aggcgggcgt ggagaaaatt 3660
ctgtctgcac ttgaaatccc ggatgtgggt aatttatccc aggtggttgt gatgaaaagt 3720
aaaaatgacc aagggatcac caataaatgc aaaatgaatc tgcaagataa caacggcaac 3780
gacattggtt ttatcggctt ccaccaattc aataatatcg cgaagcttgt ggcctcaaat 3840
tggtacaacc gtcagattga gcgcagctcc cgcactttag gctgtagctg ggagttcatt 3900
ccggtagatg acggttgggg agaacgccca ttgcaccatc atcaccatca ctgagcggcc 3960
gcataatgct taagtcgaac agattgatat gtagctataa gtaatcgtat tgtacacggc 4020
cgcataatcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc 4080
atcttagtat attagttaag tataagaagg agatatacca tgggcgaatc tctgttcaag 4140
ggtccgcgtg attataaccc gatatcttct tctatttgtc atctgactaa cgaaagcgac 4200
ggccacacga cttctctgta cggtatcggt ttcggtccgt tcatcattac caacaagcat 4260
ctgttccgcc gtaacaacgg taccctgctg gttcaatctc tgcacggcgt cttcaaggta 4320
aaagacacca ctacgctgca gcagcacctg gtcgacggcc gtgacatgat catcatccgc 4380
atgccgaaag attttccgcc gttcccgcaa aaactgaagt ttcgtgaacc gcaacgcgaa 4440
gaacgtattt gcctggttac caccaacttt cagaccaaaa gcatgtcttc tatggtttcc 4500
gatacctctt gcaccttccc aagcggtgac ggtattttct ggaaacattg gattcagacc 4560
aaagatggtc agtgcggctc tccgctggtg tctacgcgtg acggtttcat cgttggtatc 4620
cattctgctt ctaacttcac taacactaac aactacttta cttccgttcc gaaaaacttc 4680
atggagctgc tgactaacca agaggcccag cagtgggtgt ccggttggcg cctgaacgca 4740
gattctgtac tgtggggtgg tcataaggta ttcatgaaca aaccggagga gccgttccag 4800
ccggtcaaag aggcgaccca gctgatgaac gaactggttt actctcagta a          4851

<210> 91
<211> 732
<212> DNA
<213> Artificial Sequence

<220> 
<223> TEV variant 7

<400> 91
atgggcgaat ctctgttcaa gggtccgcgt gattataacc cgatatcttc ttctatttgt 60
catctgacta acgaaagcga cggccacacg acttctctgt acggtatcgg tttcggtccg 120
ttcatcatta ccaacaagca tctgttccgc cgtaacaacg gtaccctgct ggttcaatct 180
ctgcacggcg tcttcaaggt aaaagacacc actacgctgc agcagcacct ggtcgacggc 240
cgtgacatga tcatcatccg catgccgaaa gattttccgc cgttcccgca aaaactgaag 300
tttcgtgaac cgcaacgcga agaacgtatt tgcctggtta ccaccaactt tcagaccaaa 360
agcatgtctt ctatggtttc cgatacctct tgcaccttcc caagcggtga cggtattttc 420
tggaaacatt ggatccagac caaagatggt cagtgcggct ctccgctggt gtctacgcgt 480
gacggtttca tcgttggtat ccattctgct tctaacttca ctaacactaa caactacttt 540
acttccgttc cgaaaaactt catggagctg ctgactaacc aagaggccca gcagtgggtg 600
tccggttggc gcctgaacgc agattctgta ctgtggggtg gtcataaggt attcatgaac 660
aaaccggagg agccgttcca gccggtcaaa gaggcgaccc agctgatgaa cgaactggtt 720
tactctcagt aa                                                     732

<210> 92
<211> 415
<212> DNA
<213> Artificial Sequence

<220> 
<223> Intervening sequence containing transcriptional
      and translational sites and T7 termination site.

<400> 92
aagcttgtgg cctcaaattg gtacaaccgt cagattgagc gcagctcccg cactttaggc 60
tgtagctggg agttcattcc ggtagatgac ggttggggag aacgcccatt gcaccatcat 120
caccatcact gagcggccgc ataatgctta agtcgaacag attgatatgt agctataagt 180
aattgtatga ctgaacctag gctgctgcca ccgctgagca ataactagca taaccccttg 240
gggcctctaa acgggtcttg aggggttttt tgctgatcgt atactctcag gcatctatga 300
gttgtacacg tccgcataat cgaaattaat acgactcact ataggggaat tgtgagcgga 360
taacaattcc ccatcttagt atattagtta agtataagaa ggagatatac catgg      415

<210> 93
<211> 4965
<212> DNA
<213> Artificial Sequence

<220> 
<223> BoNT/A-TEV and TEV protease variant 4 open reading
      frames with the intervening transcription and
      translation elements and termination site.

<400> 93
atgccgttcg taaacaaaca gttcaactat aaagacccag tcaacggcgt ggacattgcc 60
tatatcaaaa tcccgaatgc gggtcaaatg cagcccgtga aagcatttaa aatccataac 120
aaaatttggg tgatcccgga gcgcgatacg ttcacgaacc cggaagaagg agatttaaac 180
ccaccgcctg aggctaaaca ggtcccggtg tcttactatg atagcacata cctgagtacc 240
gacaatgaaa aggacaacta cctgaaaggt gttaccaaac tgttcgagcg catttattcg 300
acagatctcg gtcgcatgtt gctgacttct attgtgcgcg gcattccgtt ttggggtggt 360
agcaccatcg atacagaact caaagtgatt gacaccaact gcatcaatgt gattcagcct 420
gatgggagct accggtccga agagcttaac ctcgtaatca ttggcccgag cgcggatatt 480
atccaattcg aatgtaaatc ttttgggcat gaagtcctga atctgacgcg gaatggctat 540
ggatcgacgc agtatattcg tttttctcca gatttcacat ttggatttga agaaagcctc 600
gaagttgata cgaaccctct tttaggcgcg ggaaaattcg cgacggaccc agcggtgacc 660
ttggcacatg aacttattca tgccgggcat cgcttgtatg gaatcgccat taacccgaac 720
cgtgttttca aggtgaatac gaacgcgtat tacgagatgt cgggcttaga agtgtccttt 780
gaagaactgc gcacgtttgg cggtcatgat gcaaaattta ttgatagtct gcaagaaaac 840
gaatttcggc tgtactatta caataaattc aaagacattg catcaacctt aaacaaggcg 900
aaaagcattg tgggtaccac ggctagctta caatatatga aaaacgtttt caaagaaaaa 960
tacctcctta gcgaagacac ttccggcaaa ttctctgtcg ataaactgaa atttgataaa 1020
ctgtataaaa tgctcaccga gatctacaca gaggataact ttgtcaaatt cttcaaggtc 1080
ttgaatcgga aaacctatct gaacttcgat aaagccgtct ttaagatcaa catcgtaccg 1140
aaagttaact acaccatcta tgatggcttt aatctgcgca atacgaatct ggcggcgaac 1200
tttaacggcc agaacaccga aatcaacaac atgaacttta ctaaactgaa aaattttacc 1260
ggcttgtttg aattctataa gctcctgtgt gtccgcggta ttatcaccag caaaaccaaa 1320
tccttgggcg gtggtggcga aaacctgtac ttccagggcg gtggcggtgg tgataagggc 1380
tataacaagg ccttcaatga tttatgcatc aaggtgaaca actgggactt gtttttctct 1440
ccatctgaag ataattttac taacgacttg aacaaaggag aggaaattac ttccgatacc 1500
aacatcgaag cagcggaaga gaatattagt ctagatctta ttcaacaata ttacctgacc 1560
tttaattttg ataacgagcc tgagaacatt tccattgaga atctcagctc tgacatcatc 1620
ggccagctgg aactgatgcc gaatatcgaa cgctttccta atggaaagaa atatgaattg 1680
gacaaataca ccatgttcca ctatctccgc gcgcaggagt ttgagcacgg caagtctcgt 1740
attgctctga ccaattcggt aaacgaagcc cttttaaatc cttcgcgtgt gtacaccttt 1800
ttctcaagcg attatgttaa aaaagtgaac aaggcgaccg aagcggcgat gtttttggga 1860
tgggtggaac aactggtata tgactttacg gatgaaactt ctgaagtctc gaccaccgac 1920
aaaattgccg atattaccat tatcattccc tatattggcc ctgcactgaa cattggtaac 1980
atgctgtata aagatgattt tgtgggcgcc ctgatctttt caggcgctgt tatcctgctg 2040
gaatttatcc cggaaatcgc cattccagta ctcggtacct ttgcgctggt gtcctatatc 2100
gcaaacaaag ttttgactgt ccagacgatc gacaacgcgc tcagtaaacg taacgaaaaa 2160
tgggatgagg tgtataagta tattgttacc aactggctcg ctaaagtaaa cacccagatt 2220
gacctgattc gcaagaagat gaaagaagcg ctggaaaacc aagcagaagc gaccaaagct 2280
attatcaact atcaatataa ccagtacaca gaggaagaaa agaataacat caacttcaac 2340
atcgacgact tatcttcaaa gctgaatgaa tctattaaca aagcgatgat taatattaac 2400
aagttcttga accaatgtag tgtcagctat ctgatgaact cgatgatccc ttacggtgtg 2460
aaacgtctgg aagacttcga tgcaagcctt aaagatgccc ttctgaagta tatttacgat 2520
aatcgcggaa ctcttattgg ccaagtggat cgcttaaaag ataaagtcaa caacacgctg 2580
agtacagaca tcccttttca gctgtctaaa tatgtggaca atcagcgcct gctgtccacg 2640
tttacggaat acatcaaaaa catcatcaac actagtattc tgaacttgcg ttacgagagt 2700
aaccatctga ttgatctgag ccgttacgca tctaaaatca acatcggatc caaggtgaac 2760
ttcgatccta tcgacaaaaa ccagattcaa ttgttcaact tagaatcgtc aaagattgaa 2820
gttatcttaa aaaatgcgat tgtatataat tcaatgtacg aaaatttctc tacgagcttt 2880
tggattcgta ttccgaaata tttcaacagt atctctttaa acaacgagta tactatcatc 2940
aattgtatgg agaataacag cgggtggaaa gtgagcctta actatggtga aatcatctgg 3000
actctgcagg acactcaaga aattaaacaa cgcgtggtgt ttaaatactc acagatgatt 3060
aacatctcgg attatattaa tcgctggatt tttgtgacaa ttactaacaa ccggctgaac 3120
aacagcaaaa tttacattaa cggtcgcctg atcgatcaga aaccaatcag taatctcggt 3180
aacattcacg catcgaataa tatcatgttc aaactggatg gttgtcgcga cacgcaccgt 3240
tacatttgga tcaaatactt caatttattc gacaaagaac tcaacgaaaa ggagattaag 3300
gatctttatg acaatcagtc taattcgggt attctgaaag acttttgggg tgattacctt 3360
cagtacgata aaccgtatta tatgttaaac ttatatgatc cgaataaata cgttgacgtc 3420
aacaacgttg gcattcgtgg ctatatgtat ctgaaagggc cgcgtggcag cgtgatgacc 3480
actaacattt acttaaactc ctccctctat cgcggtacta aatttattat caagaaatat 3540
gcctctggca acaaggacaa tatcgtacgc aataacgatc gcgtctacat taacgtggtg 3600
gtgaagaata aagaatatcg tctggcgacc aatgctagtc aggcgggcgt ggagaaaatt 3660
ctgtctgcac ttgaaatccc ggatgtgggt aatttatccc aggtggttgt gatgaaaagt 3720
aaaaatgacc aagggatcac caataaatgc aaaatgaatc tgcaagataa caacggcaac 3780
gacattggtt ttatcggctt ccaccaattc aataatatcg cgaagcttgt ggcctcaaat 3840
tggtacaacc gtcagattga gcgcagctcc cgcactttag gctgtagctg ggagttcatt 3900
ccggtagatg acggttgggg agaacgccca ttgcaccatc atcaccatca ctgagcggcc 3960
gcataatgct taagtcgaac agattgatat gtagctataa gtaattgtat gactgaacct 4020
aggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct 4080
tgaggggttt tttgctgatc gtatactctc aggcatctat gagttgtaca cgtccgcata 4140
atcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt ccccatctta 4200
gtatattagt taagtataag aaggagatat accatgggcg aatctctgtt caagggtccg 4260
cgtgattata acccgatatc ttcttctatt tgtcatctga ctaacgaaag cgacggccac 4320
acgacttctc tgtacggtat cggtttcggt ccgttcatca ttaccaacaa gcatctgttc 4380
cgccgtaaca acggtaccct gctggttcaa tctctgcacg gcgtcttcaa ggtaaaagac 4440
accactacgc tgcagcagca cctggtcgac ggccgtgaca tgatcatcat ccgcatgccg 4500
aaagattttc cgccgttccc gcaaaaactg aagtttcgtg aaccgcaacg cgaagaacgt 4560
atttgcctgg ttaccaccaa ctttcagacc aaaagcatgt cttctatggt ttccgatacc 4620
tcttgcacct tcccaagcgg tgacggtatt ttctggaaac attggattca gaccaaagat 4680
ggtcagtgcg gctctccgct ggtgtctacg cgtgacggtt tcatcgttgg tatccattct 4740
gcttctaact tcactaacac taacaactac tttacttccg ttccgaaaaa cttcatggag 4800
ctgctgacta accaagaggc ccagcagtgg gtgtccggtt ggcgcctgaa cgcagattct 4860
gtactgtggg gtggtcataa ggtattcatg aacaaaccgg aggagccgtt ccagccggtc 4920
aaagaggcga cccagctgat gaacgaactg gtttactctc agtaa                 4965

<210> 94
<211> 2697
<212> DNA
<213> Artificial Sequence

<220> 
<223> Open reading frame for NociLHN/A-TEV

<400> 94
atgccgttcg taaacaaaca gttcaactat aaagacccag tcaacggcgt ggacattgcc 60
tatatcaaaa tcccgaatgc gggtcaaatg cagcccgtga aagcatttaa aatccataac 120
aaaatttggg tgatcccgga gcgcgatacg ttcacgaacc cggaagaagg agatttaaac 180
ccaccgcctg aggctaaaca ggtcccggtg tcttactatg atagcacata cctgagtacc 240
gacaatgaaa aggacaacta cctgaaaggt gttaccaaac tgttcgagcg catttattcg 300
acagatctcg gtcgcatgtt gctgacttct attgtgcgcg gcattccgtt ttggggtggt 360
agcaccatcg atacagaact caaagtgatt gacaccaact gcatcaatgt gattcagcct 420
gatgggagct accggtccga agagcttaac ctcgtaatca ttggcccgag cgcggatatt 480
atccaattcg aatgtaaatc ttttgggcat gaagtcctga atctgacgcg gaatggctat 540
ggatcgacgc agtatattcg tttttctcca gatttcacat ttggatttga agaaagcctc 600
gaagttgata cgaaccctct tttaggcgcg ggaaaattcg cgacggaccc agcggtgacc 660
ttggcacatg aacttattca tgccgggcat cgcttgtatg gaatcgccat taacccgaac 720
cgtgttttca aggtgaatac gaacgcgtat tacgagatgt cgggcttaga agtgtccttt 780
gaagaactgc gcacgtttgg cggtcatgat gcaaaattta ttgatagtct gcaagaaaac 840
gaatttcggc tgtactatta caataaattc aaagacattg catcaacctt aaacaaggcg 900
aaaagcattg tgggtaccac ggctagctta caatatatga aaaacgtttt caaagaaaaa 960
tacctcctta gcgaagacac ttccggcaaa ttctctgtcg ataaactgaa atttgataaa 1020
ctgtataaaa tgctcaccga gatctacaca gaggataact ttgtcaaatt cttcaaggtc 1080
ttgaatcgga aaacctatct gaacttcgat aaagccgtct ttaagatcaa catcgtaccg 1140
aaagttaact acaccatcta tgatggcttt aatctgcgca atacgaatct ggcggcgaac 1200
tttaacggcc agaacaccga aatcaacaac atgaacttta ctaaactgaa aaattttacc 1260
ggcttgtttg aattctataa gctcctgtgt gtccgcggta ttatcaccag caaagaaaac 1320
ctgtacttcc agttcggtgg ttttaccggc gctcgtaaat ctgcacgtaa acgcaagaat 1380
caggctctgg ctggtggcgg tggctctggt ggtggcggta gcggcggtgg cggttctgcg 1440
ctcaatgatt tatgcatcaa ggtgaacaac tgggacttgt ttttctctcc atctgaagat 1500
aattttacta acgacttgaa caaaggagag gaaattactt ccgataccaa catcgaagca 1560
gcggaagaga atattagtct agatcttatt caacaatatt acctgacctt taattttgat 1620
aacgagcctg agaacatttc cattgagaat ctcagctctg acatcatcgg ccagctggaa 1680
ctgatgccga atatcgaacg ctttcctaat ggaaagaaat atgaattgga caaatacacc 1740
atgttccact atctccgcgc gcaggagttt gagcacggca agtctcgtat tgctctgacc 1800
aattcggtaa acgaagccct tttaaatcct tcgcgtgtgt acaccttttt ctcaagcgat 1860
tatgttaaaa aagtgaacaa ggcgaccgaa gcggcgatgt ttttgggatg ggtggaacaa 1920
ctggtatatg actttacgga tgaaacttct gaagtctcga ccaccgacaa aattgccgat 1980
attaccatta tcattcccta tattggccct gcactgaaca ttggtaacat gctgtataaa 2040
gatgattttg tgggcgccct gatcttttca ggcgctgtta tcctgctgga atttatcccg 2100
gaaatcgcca ttccagtact cggtaccttt gcgctggtgt cctatatcgc aaacaaagtt 2160
ttgactgtcc agacgatcga caacgcgctc agtaaacgta acgaaaaatg ggatgaggtg 2220
tataagtata ttgttaccaa ctggctcgct aaagtaaaca cccagattga cctgattcgc 2280
aagaagatga aagaagcgct ggaaaaccaa gcagaagcga ccaaagctat tatcaactat 2340
caatataacc agtacacaga ggaagaaaag aataacatca acttcaacat cgacgactta 2400
tcttcaaagc tgaatgaatc tattaacaaa gcgatgatta atattaacaa gttcttgaac 2460
caatgtagtg tcagctatct gatgaactcg atgatccctt acggtgtgaa acgtctggaa 2520
gacttcgatg caagccttaa agatgccctt ctgaagtata tttacgataa tcgcggaact 2580
cttattggcc aagtggatcg cttaaaagat aaagtcaaca acacgctgag tacagacatc 2640
ccttttcagc tgtctaaata tgtggacaat cagcgccacc atcaccatca ccactaa    2697

<210> 95
<211> 898
<212> PRT
<213> Artificial Sequence

<220> 
<223> NociLHN/A-TEV

<400> 95
Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly
 1               5                  10                  15      
Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro
            20                  25                  30          
Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg
        35                  40                  45              
Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu
    50                  55                  60                  
Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr
65                  70                  75                  80  
Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu
                85                  90                  95      
Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val
            100                 105                 110         
Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys
        115                 120                 125             
Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr
    130                 135                 140                 
Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile
145                 150                 155                 160 
Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr
                165                 170                 175     
Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe
            180                 185                 190         
Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu
        195                 200                 205             
Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu
    210                 215                 220                 
Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn
225                 230                 235                 240 
Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu
                245                 250                 255     
Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys
            260                 265                 270         
Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn
        275                 280                 285             
Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val
    290                 295                 300                 
Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys
305                 310                 315                 320 
Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu
                325                 330                 335     
Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp
            340                 345                 350         
Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn
        355                 360                 365             
Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr
    370                 375                 380                 
Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn
385                 390                 395                 400 
Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu
                405                 410                 415     
Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg
            420                 425                 430         
Gly Ile Ile Thr Ser Lys Glu Asn Leu Tyr Phe Gln Phe Gly Gly Phe
        435                 440                 445             
Thr Gly Ala Arg Lys Ser Ala Arg Lys Arg Lys Asn Gln Ala Leu Ala
    450                 455                 460                 
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala
465                 470                 475                 480 
Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe Ser
                485                 490                 495     
Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu Ile
            500                 505                 510         
Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu Asp
        515                 520                 525             
Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro Glu
    530                 535                 540                 
Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu Glu
545                 550                 555                 560 
Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu Leu
                565                 570                 575     
Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu His
            580                 585                 590         
Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu Leu
        595                 600                 605             
Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys Lys
    610                 615                 620                 
Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu Gln
625                 630                 635                 640 
Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr Asp
                645                 650                 655     
Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala Leu
            660                 665                 670         
Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu Ile
        675                 680                 685             
Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala Ile
    690                 695                 700                 
Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys Val
705                 710                 715                 720 
Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu Lys
                725                 730                 735     
Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys Val
            740                 745                 750         
Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu Glu
        755                 760                 765             
Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn Gln
    770                 775                 780                 
Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp Leu
785                 790                 795                 800 
Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile Asn
                805                 810                 815     
Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met Ile
            820                 825                 830         
Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys Asp
        835                 840                 845             
Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly Gln
    850                 855                 860                 
Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp Ile
865                 870                 875                 880 
Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg His His His His
                885                 890                 895     
His His
        


<210> 96
<211> 2709
<212> DNA
<213> Artificial Sequence

<220> 
<223> Open reading frame for DynLHN/A-TEV

<400> 96
atgccgttcg taaacaaaca gttcaactat aaagacccag tcaacggcgt ggacattgcc 60
tatatcaaaa tcccgaatgc gggtcaaatg cagcccgtga aagcatttaa aatccataac 120
aaaatttggg tgatcccgga gcgcgatacg ttcacgaacc cggaagaagg agatttaaac 180
ccaccgcctg aggctaaaca ggtcccggtg tcttactatg atagcacata cctgagtacc 240
gacaatgaaa aggacaacta cctgaaaggt gttaccaaac tgttcgagcg catttattcg 300
acagatctcg gtcgcatgtt gctgacttct attgtgcgcg gcattccgtt ttggggtggt 360
agcaccatcg atacagaact caaagtgatt gacaccaact gcatcaatgt gattcagcct 420
gatgggagct accggtccga agagcttaac ctcgtaatca ttggcccgag cgcggatatt 480
atccaattcg aatgtaaatc ttttgggcat gaagtcctga atctgacgcg gaatggctat 540
ggatcgacgc agtatattcg tttttctcca gatttcacat ttggatttga agaaagcctc 600
gaagttgata cgaaccctct tttaggcgcg ggaaaattcg cgacggaccc agcggtgacc 660
ttggcacatg aacttattca tgccgggcat cgcttgtatg gaatcgccat taacccgaac 720
cgtgttttca aggtgaatac gaacgcgtat tacgagatgt cgggcttaga agtgtccttt 780
gaagaactgc gcacgtttgg cggtcatgat gcaaaattta ttgatagtct gcaagaaaac 840
gaatttcggc tgtactatta caataaattc aaagacattg catcaacctt aaacaaggcg 900
aaaagcattg tgggtaccac ggctagctta caatatatga aaaacgtttt caaagaaaaa 960
tacctcctta gcgaagacac ttccggcaaa ttctctgtcg ataaactgaa atttgataaa 1020
ctgtataaaa tgctcaccga gatctacaca gaggataact ttgtcaaatt cttcaaggtc 1080
ttgaatcgga aaacctatct gaacttcgat aaagccgtct ttaagatcaa catcgtaccg 1140
aaagttaact acaccatcta tgatggcttt aatctgcgca atacgaatct ggcggcgaac 1200
tttaacggcc agaacaccga aatcaacaac atgaacttta ctaaactgaa aaattttacc 1260
ggcttgtttg aattctataa gctcctgtgt gtccgtggta ttatcaccag caaagaaaac 1320
ctgtacttcc agtatggcgg tttcctgcgt cgcattcgtc ctaagcttaa atgggataac 1380
caggctcttg ctggtggtgg tggctctggt ggtggcggta gcggcggtgg tggttctgca 1440
ctcaatgatt tatgtatcaa ggtgaacaac tgggacttgt ttttctctcc atctgaagat 1500
aattttacta acgacttgaa caaaggagag gaaattactt ccgataccaa catcgaagca 1560
gcggaagaga atattagtct agatcttatt caacaatatt acctgacctt taattttgat 1620
aacgagcctg agaacatttc cattgagaat ctcagctctg acatcatcgg ccagctggaa 1680
ctgatgccga atatcgaacg ctttcctaat ggaaagaaat atgaattgga caaatacacc 1740
atgttccact atctccgcgc gcaggagttt gagcacggca agtctcgtat tgctctgacc 1800
aattcggtaa acgaagccct tttaaatcct tcgcgtgtgt acaccttttt ctcaagcgat 1860
tatgttaaaa aagtgaacaa ggcgaccgaa gcggcgatgt ttttgggatg ggtggaacaa 1920
ctggtatatg actttacgga tgaaacttct gaagtctcga ccaccgacaa aattgccgat 1980
attaccatta tcattcccta tattggccct gcactgaaca ttggtaacat gctgtataaa 2040
gatgattttg tgggcgccct gatcttttca ggcgctgtta tcctgctgga atttatcccg 2100
gaaatcgcca ttccagtact cggtaccttt gcgctggtgt cctatatcgc aaacaaagtt 2160
ttgactgtcc agacgatcga caacgcgctc agtaaacgta acgaaaaatg ggatgaggtg 2220
tataagtata ttgttaccaa ctggctcgct aaagtaaaca cccagattga cctgattcgc 2280
aagaagatga aagaagcgct ggaaaaccaa gcagaagcga ccaaagctat tatcaactat 2340
caatataacc agtacacaga ggaagaaaag aataacatca acttcaacat cgacgactta 2400
tcttcaaagc tgaatgaatc tattaacaaa gcgatgatta atattaacaa gttcttgaac 2460
caatgtagtg tcagctatct gatgaactcg atgatccctt acggtgtgaa acgtctggaa 2520
gacttcgatg caagccttaa agatgccctt ctgaagtata tttacgataa tcgcggaact 2580
cttattggcc aagtggatcg cttaaaagat aaagtcaaca acacgctgag tacagacatc 2640
ccttttcagc tgtctaaata tgtggacaat cagcgcctgc tgtccacgca ccatcaccat 2700
caccactaa                                                         2709

<210> 97
<211> 902
<212> PRT
<213> Artificial Sequence

<220> 
<223> DynLHN/A-TEV

<400> 97
Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly
 1               5                  10                  15      
Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro
            20                  25                  30          
Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg
        35                  40                  45              
Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu
    50                  55                  60                  
Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr
65                  70                  75                  80  
Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu
                85                  90                  95      
Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val
            100                 105                 110         
Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys
        115                 120                 125             
Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr
    130                 135                 140                 
Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile
145                 150                 155                 160 
Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr
                165                 170                 175     
Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe
            180                 185                 190         
Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu
        195                 200                 205             
Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu
    210                 215                 220                 
Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn
225                 230                 235                 240 
Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu
                245                 250                 255     
Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys
            260                 265                 270         
Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn
        275                 280                 285             
Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val
    290                 295                 300                 
Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys
305                 310                 315                 320 
Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu
                325                 330                 335     
Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp
            340                 345                 350         
Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn
        355                 360                 365             
Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr
    370                 375                 380                 
Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn
385                 390                 395                 400 
Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu
                405                 410                 415     
Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg
            420                 425                 430         
Gly Ile Ile Thr Ser Lys Glu Asn Leu Tyr Phe Gln Tyr Gly Gly Phe
        435                 440                 445             
Leu Arg Arg Ile Arg Pro Lys Leu Lys Trp Asp Asn Gln Ala Leu Ala
    450                 455                 460                 
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala
465                 470                 475                 480 
Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe Ser
                485                 490                 495     
Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu Ile
            500                 505                 510         
Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu Asp
        515                 520                 525             
Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro Glu
    530                 535                 540                 
Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu Glu
545                 550                 555                 560 
Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu Leu
                565                 570                 575     
Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu His
            580                 585                 590         
Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu Leu
        595                 600                 605             
Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys Lys
    610                 615                 620                 
Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu Gln
625                 630                 635                 640 
Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr Asp
                645                 650                 655     
Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala Leu
            660                 665                 670         
Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu Ile
        675                 680                 685             
Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala Ile
    690                 695                 700                 
Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys Val
705                 710                 715                 720 
Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu Lys
                725                 730                 735     
Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys Val
            740                 745                 750         
Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu Glu
        755                 760                 765             
Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn Gln
    770                 775                 780                 
Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp Leu
785                 790                 795                 800 
Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile Asn
                805                 810                 815     
Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met Ile
            820                 825                 830         
Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys Asp
        835                 840                 845             
Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly Gln
    850                 855                 860                 
Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp Ile
865                 870                 875                 880 
Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser Thr
                885                 890                 895     
His His His His His His
            900         


<210> 98
<211> 320
<212> DNA
<213> Artificial Sequence

<220> 
<223> DNA fragment encoding a di-chain loop region
      comprising an integrated TEV protease cleavage
      site-Galanin binding domain

<400> 98
gaattctaca agctgctgtg cgtggacggc atcattacct ccaaaactaa atctgaaaac 60
ctgtacttcc agggctggac tttgaactct gctggttatc tcctgggccc acatgcggtt 120
gctcttgctg gtggcggtgg ctctggcggt ggcggtagcg gcggtggcgg ttctgcacta 180
gtgcttcagt gtatcaaggt taacaactgg gatttattct tcagcccgag tgaagacaac 240
ttcaccaacg acctgaacaa aggtgaagaa atcacctcag atactaacat cgaagcagcc 300
gaagaaaaca tcagtctaga                                             320

<210> 99
<211> 106
<212> PRT
<213> Artificial Sequence

<220> 
<223> Di-chain loop region comprising an integrated TEV
      protease cleavage site-Galanin binding domain

<400> 99
Glu Phe Tyr Lys Leu Leu Cys Val Asp Gly Ile Ile Thr Ser Lys Thr
 1               5                  10                  15      
Lys Ser Glu Asn Leu Tyr Phe Gln Gly Trp Thr Leu Asn Ser Ala Gly
            20                  25                  30          
Tyr Leu Leu Gly Pro His Ala Val Ala Leu Ala Gly Gly Gly Gly Ser
        35                  40                  45              
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Leu Val Leu Gln Cys
    50                  55                  60                  
Ile Lys Val Asn Asn Trp Asp Leu Phe Phe Ser Pro Ser Glu Asp Asn
65                  70                  75                  80  
Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu Ile Thr Ser Asp Thr Asn
                85                  90                  95      
Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu
            100                 105     


<210> 100
<211> 2703
<212> DNA
<213> Artificial Sequence

<220> 
<223> Open reading frame for GalLHN/A-TEV

<400> 100
atgccgttcg taaacaaaca gttcaactat aaagacccag tcaacggcgt ggacattgcc 60
tatatcaaaa tcccgaatgc gggtcaaatg cagcccgtga aagcatttaa aatccataac 120
aaaatttggg tgatcccgga gcgcgatacg ttcacgaacc cggaagaagg agatttaaac 180
ccaccgcctg aggctaaaca ggtcccggtg tcttactatg atagcacata cctgagtacc 240
gacaatgaaa aggacaacta cctgaaaggt gttaccaaac tgttcgagcg catttattcg 300
acagatctcg gtcgcatgtt gctgacttct attgtgcgcg gcattccgtt ttggggtggt 360
agcaccatcg atacagaact caaagtgatt gacaccaact gcatcaatgt gattcagcct 420
gatgggagct accggtccga agagcttaac ctcgtaatca ttggcccgag cgcggatatt 480
atccaattcg aatgtaaatc ttttgggcat gaagtcctga atctgacgcg gaatggctat 540
ggatcgacgc agtatattcg tttttctcca gatttcacat ttggatttga agaaagcctc 600
gaagttgata cgaaccctct tttaggcgcg ggaaaattcg cgacggaccc agcggtgacc 660
ttggcacatg aacttattca tgccgggcat cgcttgtatg gaatcgccat taacccgaac 720
cgtgttttca aggtgaatac gaacgcgtat tacgagatgt cgggcttaga agtgtccttt 780
gaagaactgc gcacgtttgg cggtcatgat gcaaaattta ttgatagtct gcaagaaaac 840
gaatttcggc tgtactatta caataaattc aaagacattg catcaacctt aaacaaggcg 900
aaaagcattg tgggtaccac ggctagctta caatatatga aaaacgtttt caaagaaaaa 960
tacctcctta gcgaagacac ttccggcaaa ttctctgtcg ataaactgaa atttgataaa 1020
ctgtataaaa tgctcaccga gatctacaca gaggataact ttgtcaaatt cttcaaggtc 1080
ttgaatcgga aaacctatct gaacttcgat aaagccgtct ttaagatcaa catcgtaccg 1140
aaagttaact acaccatcta tgatggcttt aatctgcgca atacgaatct ggcggcgaac 1200
tttaacggcc agaacaccga aatcaacaac atgaacttta ctaaactgaa aaattttacc 1260
ggcttgtttg aattctacaa gctgctgtgc gtggacggca tcattacctc caaaactaaa 1320
tctgaaaacc tgtacttcca gggctggact ttgaactctg ctggttatct cctgggccca 1380
catgcggttg ctcttgctgg tggcggtggc tctggcggtg gcggtagcgg cggtggcggt 1440
tctgcactag tgcttcagtg tatcaaggtt aacaactggg atttattctt cagcccgagt 1500
gaagacaact tcaccaacga cctgaacaaa ggtgaagaaa tcacctcaga tactaacatc 1560
gaagcagccg aagaaaacat cagtctagat cttattcaac aatattacct gacctttaat 1620
tttgataacg agcctgagaa catttccatt gagaatctca gctctgacat catcggccag 1680
ctggaactga tgccgaatat cgaacgcttt cctaatggaa agaaatatga attggacaaa 1740
tacaccatgt tccactatct ccgcgcgcag gagtttgagc acggcaagtc tcgtattgct 1800
ctgaccaatt cggtaaacga agccctttta aatccttcgc gtgtgtacac ctttttctca 1860
agcgattatg ttaaaaaagt gaacaaggcg accgaagcgg cgatgttttt gggatgggtg 1920
gaacaactgg tatatgactt tacggatgaa acttctgaag tctcgaccac cgacaaaatt 1980
gccgatatta ccattatcat tccctatatt ggccctgcac tgaacattgg taacatgctg 2040
tataaagatg attttgtggg cgccctgatc ttttcaggcg ctgttatcct gctggaattt 2100
atcccggaaa tcgccattcc agtactcggt acctttgcgc tggtgtccta tatcgcaaac 2160
aaagttttga ctgtccagac gatcgacaac gcgctcagta aacgtaacga aaaatgggat 2220
gaggtgtata agtatattgt taccaactgg ctcgctaaag taaacaccca gattgacctg 2280
attcgcaaga agatgaaaga agcgctggaa aaccaagcag aagcgaccaa agctattatc 2340
aactatcaat ataaccagta cacagaggaa gaaaagaata acatcaactt caacatcgac 2400
gacttatctt caaagctgaa tgaatctatt aacaaagcga tgattaatat taacaagttc 2460
ttgaaccaat gtagtgtcag ctatctgatg aactcgatga tcccttacgg tgtgaaacgt 2520
ctggaagact tcgatgcaag ccttaaagat gcccttctga agtatattta cgataatcgc 2580
ggaactctta ttggccaagt ggatcgctta aaagataaag tcaacaacac gctgagtaca 2640
gacatccctt ttcagctgtc taaatatgtg gacaatcagc gccaccatca ccatcaccac 2700
taa                                                               2703

<210> 101
<211> 900
<212> PRT
<213> Artificial Sequence

<220> 
<223> GalLHN/A-TEV

<400> 101
Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly
 1               5                  10                  15      
Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro
            20                  25                  30          
Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg
        35                  40                  45              
Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu
    50                  55                  60                  
Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr
65                  70                  75                  80  
Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu
                85                  90                  95      
Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val
            100                 105                 110         
Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys
        115                 120                 125             
Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr
    130                 135                 140                 
Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile
145                 150                 155                 160 
Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr
                165                 170                 175     
Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe
            180                 185                 190         
Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu
        195                 200                 205             
Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu
    210                 215                 220                 
Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn
225                 230                 235                 240 
Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu
                245                 250                 255     
Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys
            260                 265                 270         
Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn
        275                 280                 285             
Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val
    290                 295                 300                 
Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys
305                 310                 315                 320 
Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu
                325                 330                 335     
Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp
            340                 345                 350         
Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn
        355                 360                 365             
Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr
    370                 375                 380                 
Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn
385                 390                 395                 400 
Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu
                405                 410                 415     
Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Asp
            420                 425                 430         
Gly Ile Ile Thr Ser Lys Thr Lys Ser Glu Asn Leu Tyr Phe Gln Gly
        435                 440                 445             
Trp Thr Leu Asn Ser Ala Gly Tyr Leu Leu Gly Pro His Ala Val Ala
    450                 455                 460                 
Leu Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
465                 470                 475                 480 
Ser Ala Leu Val Leu Gln Cys Ile Lys Val Asn Asn Trp Asp Leu Phe
                485                 490                 495     
Phe Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu
            500                 505                 510         
Glu Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser
        515                 520                 525             
Leu Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu
    530                 535                 540                 
Pro Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln
545                 550                 555                 560 
Leu Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr
                565                 570                 575     
Glu Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe
            580                 585                 590         
Glu His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala
        595                 600                 605             
Leu Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val
    610                 615                 620                 
Lys Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val
625                 630                 635                 640 
Glu Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr
                645                 650                 655     
Thr Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro
            660                 665                 670         
Ala Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala
        675                 680                 685             
Leu Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile
    690                 695                 700                 
Ala Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn
705                 710                 715                 720 
Lys Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn
                725                 730                 735     
Glu Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala
            740                 745                 750         
Lys Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala
        755                 760                 765             
Leu Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr
    770                 775                 780                 
Asn Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp
785                 790                 795                 800 
Asp Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn
                805                 810                 815     
Ile Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser
            820                 825                 830         
Met Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu
        835                 840                 845             
Lys Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile
    850                 855                 860                 
Gly Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr
865                 870                 875                 880 
Asp Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg His His
                885                 890                 895     
His His His His
            900 


<210> 102
<211> 314
<212> DNA
<213> Artificial Sequence

<220> 
<223> DNA fragment encoding a di-chain loop region
      comprising an integrated TEV protease cleavage
      site-Nociceptin binding domain

<400> 102
gaattctata agctcctgtg tgtccgcggt attatcacca gcaaagaaaa cctgtacttc 60
cagttcggtg gttttaccgg cgctcgtaaa tctgcacgta aacgcaagaa tcaggctctg 120
gctggtggcg gtggctctgg tggtggcggt agcggcggtg gcggttctgc gctcaatgat 180
ttatgcatca aggtgaacaa ctgggacttg tttttctctc catctgaaga taattttact 240
aacgacttga acaaaggaga ggaaattact tccgatacca acatcgaagc agcggaagag 300
aatattagtc taga                                                   314

<210> 103
<211> 104
<212> PRT
<213> Artificial Sequence

<220> 
<223> Di-chain loop region comprising an integrated TEV
      protease cleavage site-Nociceptin binding domain

<400> 103
Glu Phe Tyr Lys Leu Leu Cys Val Arg Gly Ile Ile Thr Ser Lys Glu
 1               5                  10                  15      
Asn Leu Tyr Phe Gln Phe Gly Gly Phe Thr Gly Ala Arg Lys Ser Ala
            20                  25                  30          
Arg Lys Arg Lys Asn Gln Ala Leu Ala Gly Gly Gly Gly Ser Gly Gly
        35                  40                  45              
Gly Gly Ser Gly Gly Gly Gly Ser Ala Leu Asn Asp Leu Cys Ile Lys
    50                  55                  60                  
Val Asn Asn Trp Asp Leu Phe Phe Ser Pro Ser Glu Asp Asn Phe Thr
65                  70                  75                  80  
Asn Asp Leu Asn Lys Gly Glu Glu Ile Thr Ser Asp Thr Asn Ile Glu
                85                  90                  95      
Ala Ala Glu Glu Asn Ile Ser Leu
            100                 


<210> 104
<211> 314
<212> DNA
<213> Artificial Sequence

<220> 
<223> DNA fragment encoding a di-chain loop region
      comprising an integrated TEV protease cleavage
      site-Dynorphin binding domain

<400> 104
gaattctata agctcctgtg tgtccgtggt attatcacca gcaaagaaaa cctgtacttc 60
cagtatggcg gtttcctgcg tcgcattcgt cctaagctta aatgggataa ccaggctctt 120
gctggtggtg gtggctctgg tggtggcggt agcggcggtg gtggttctgc actcaatgat 180
ttatgtatca aggtgaacaa ctgggacttg tttttctctc catctgaaga taattttact 240
aacgacttga acaaaggaga ggaaattact tccgatacca acatcgaagc agcggaagag 300
aatattagtc taga                                                   314

<210> 105
<211> 104
<212> PRT
<213> Artificial Sequence

<220> 
<223> Di-chain loop region comprising an integrated TEV
      protease cleavage site-Dynorphin binding domain

<400> 105
Glu Phe Tyr Lys Leu Leu Cys Val Arg Gly Ile Ile Thr Ser Lys Glu
 1               5                  10                  15      
Asn Leu Tyr Phe Gln Tyr Gly Gly Phe Leu Arg Arg Ile Arg Pro Lys
            20                  25                  30          
Leu Lys Trp Asp Asn Gln Ala Leu Ala Gly Gly Gly Gly Ser Gly Gly
        35                  40                  45              
Gly Gly Ser Gly Gly Gly Gly Ser Ala Leu Asn Asp Leu Cys Ile Lys
    50                  55                  60                  
Val Asn Asn Trp Asp Leu Phe Phe Ser Pro Ser Glu Asp Asn Phe Thr
65                  70                  75                  80  
Asn Asp Leu Asn Lys Gly Glu Glu Ile Thr Ser Asp Thr Asn Ile Glu
                85                  90                  95      
Ala Ala Glu Glu Asn Ile Ser Leu
            100                 


<210>  106
<211>  750
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Open reading frame encoding TEV protease variant 7

<400>  106
ccatggatgg gtggcgaatc tctgttcaag ggtccgcgtg attataaccc gatatcttct       60
actatttgtc atctgactaa cgaaagcgac ggccacacga cttctctgta cggtatcggt      120
ttcggtccgt tcatcattac caacaagcat ctgttccgcc gtaacaacgg taccctgctg      180
gttcaatctc tgcacggcgt cttcaaggta aaagacacca ctacgctgca gcagcacctg      240
gtcgacggcc gtgacatgat catcatccgc atgccgaaag attttccgcc gttcccgcaa      300
aaactgaagt ttcgtgaacc gcaacgcgaa gaacgtattt gcctggttac caccaacttt      360
cagaccaaaa gcatgtcttc tatggtttcc gatacctctt gcaccttccc aagcggtgac      420
ggtattttct ggaaacattg gatccagacc aaagatggtc agtgcggctc tccgctggtg      480
tctacgcgtg acggtttcat cgttggtatc cattctgctt ctaacttcac taacactaac      540
aactacttta cttccgttcc gaaaaacttc atggagctgc tgactaacca agaggcccag      600
cagtgggtgt ccggttggcg cctgaacgca gattctgtac tgtggggtgg tcataaggta      660
ttcatgaaca aaccggagga gccgttccag ccggtcaaag aggcgaccca gctgatgaac      720
gaactggttt actctcagta atgaaagctt                                       750


<210>  107
<211>  3791
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF encoding p10-TEV variant 7 and polH-DynLHn/A-TEV

<400>  107
agatcttatg cggccgcact cgagtcatta gtggtgatgg tgatggtggg ttgacagaag       60
tctctgatta tcaacatact tcgacagttg gaacgggatg tctgttgaca gagtgttgtt      120
taccttatct ttcagacggt caacttggcc aatgagcgtt cccctgttat cgtagatgta      180
cttaagaagg gcgtctttca gcgaggcgtc gaagtcctcc agtctcttta caccatatgg      240
gatcattgag ttcatcaagt atgatacact gcattggttg aggaacttgt taatgtttat      300
cattgccttg tttatgctct cgttgagttt actagagagg tcgtcaatgt tgaagttgat      360
gttgttcttt tcctcctcgg tgtactggtt gtactgatag ttaatgatgg cctttgtcgc      420
ttcagcctgg ttctccagcg cctccttcat ctttttcctg atgaggtcga tttgggtgtt      480
gaccttagcg agccagttag tcacgatgta tttgtacact tcatcccact tctcgtttct      540
ttttgacaga gcattatcga ttgtttggac agtgaggacc ttgttagcaa tgtagctgac      600
caaagcgaag gtaccaagaa caggaatagc gatctctggg atgaactcca acaaaatcac      660
tgctcccgag aaaatcaacg caccgacgaa gtcgtccttg tacagcatat tgccgatgtt      720
aagagcaggt ccaatgtagg gtatgatgat agtgatgtct gcgatcttgt ccgtagtcga      780
aacttcacta gtctcgtcgg tgaaatcgta aaccaactgt tcaacccaac ccagaaacat      840
cgctgcttcg gttgccttat tcaccttctt aacgtaatcc gaactgaaga aggtgtagac      900
acgagaagga ttgagaagag cctcgttgac cgagttagtg agggcgattc tactttttcc      960
gtgctcaaac tcttgagctc tgaggtagtg gaacatcgtg tatttgtcca attcgtactt     1020
cttgccgtta gggaatctct cgatattggg catgagttcc agctgtccga tgatgtcgct     1080
gctcagattt tcgatagaaa tgttttccgg ctcgttatcg aaattgaacg tgagatagta     1140
ctgctgaatc aggtctagac taatattctc ttccgctgct tcgatgttgg tatcggaagt     1200
aatttcctct cctttgttca agtcgttagt aaaattatct tcagatggag agaaaaacaa     1260
gtcccagttg ttcaccttga tacataaatc attgagtgca gaaccaccac cgccgctacc     1320
gccaccacca gagccaccac caccagcaag agcctggtta tcccatttaa gcttaggacg     1380
aatgcgacgc aggaaaccgc catactggaa gtacaggttt tctttgctgg tgataatacc     1440
acggacacac aggagcttat agaattcaaa caggccggtg aaattcttga gctttgtgaa     1500
gttcatgtta ttgatctcgg tattctgacc attgaagtta gccgccaaat tggtgttcct     1560
aaggttaaag ccatcataga tggtgtagtt cacctttggc acgatattga tcttaaacac     1620
agctttgtcg aagttaagat aagtcttgcg gttcaatacc ttgaagaact taacaaagtt     1680
gtcctcggta tagatctctg taagcatttt gtacagcttg tcaaacttga gtttgtccac     1740
ggaaaacttt ccggaggtgt cctcggaaag caagtacttt tccttaaaga cgttcttcat     1800
atactgaagg ctagccgtgg tgccgactat acttttagcc ttattcagcg tactggcaat     1860
atctttgaat ttgttgtagt aatacagtct gaactcattc tcttgcaagg agtcgatgaa     1920
cttagcatcg tgtccaccga aggtacgaag ttcttcgaag gagacttcca gaccggacat     1980
ctcatagtat gcgttggtgt tcaccttgaa aacgcggttt ggattgatgg caattccgta     2040
cagtctatgg cctgcgtgaa tcagctcgtg agccaaggtc accgcgggat ctgtggcgaa     2100
cttgccagcg cccaacaacg gattagtgtc aacctccaat gactcttcga agccgaaagt     2160
gaaatcgggg gaaaacctga tgtattgagt agaaccataa ccgtttctgg tcaggttcag     2220
cacctcatgg ccgaaggact tacattcaaa ctgaatgatg tcggcagagg gaccgatgat     2280
caccaagttg agttcctctg aacggtagga gccgtcaggt tggatcacgt tgatacagtt     2340
tgtatcgatc actttcagct ctgtatctat ggttgatccg ccccaaaagg ggattccacg     2400
gacgatggaa gtgagcagca tgcgaccgag gtcagtggaa tagatacgct cgaaaagttt     2460
ggtcactccc ttgaggtaat tgtctttctc gttatctgtc gacaagtacg tggagtcata     2520
gtaggacacc ggcacctgct tggcctctgg tggcggattc aaatctcctt cttcggggtt     2580
agtgaaggtg tctctttcgg gaatgaccca tatcttgtta tgaatcttga aggccttaac     2640
aggctgcatt tgaccggcat tcggaatctt gatatacgca atatcgactc cgttgacagg     2700
gtccttatag ttgaattgct tgttgacaaa tcccatggga ttatatttat aggttttttt     2760
attacaaaac tgttacgaaa acagtaaaat acttatttat ttgcgagatg gttatcattt     2820
taattatctc catgatccaa taacctagaa taaaggccga cctttaattc aacccaacac     2880
aatatattat agttaaataa gaattattat caaatcattt gtatattaat taaaatacta     2940
tactgtaaat tacattttat ttacaatcac agatccatat gggcgagtca ttgttcaagg     3000
gaccgagaga ttacaacccc atctcgtcgt caatctgcca cttgacaaac gaatccgacg     3060
gtcacactac ttctctgtac ggtatcggct tcggaccttt catcatcacc aacaagcatt     3120
tgtttaggag aaacaacggt acactccttg tccagtccct gcacggcgta ttcaaagtca     3180
aagataccac gactctgcaa cagcatctgg tcgacggaag ggacatgata atcattcgca     3240
ttcctaaaga cttcccaccc ttccctcaaa agctcaagtt tcgtgagccc cagcgtgagg     3300
agaggatttg tcttgtcacg actaacttcc agaccaaatc tatgtctagc atggtcagcg     3360
atacctcgtg cacttttcca agcggcgatg gaatcttttg gaagcactgg attcagacaa     3420
aggacggcca atgcggttct cctctcgtaa gtacgcgcga cggattcatc gtgggtattc     3480
actccgcttc caacttcacc aacaccaaca actatttcac tagcgtgcca aagaatttca     3540
tggaattgct caccaaccag gaggcccaac aatgggttag tggttggcgt cttaatgcgg     3600
actcagtgct gtggggaggc cataaagttt tcatgaataa gccggaggaa ccttttcaac     3660
ccgtgaagga agcaacacag ctcatgaatg agctggttta ctcacagtga taactcgagc     3720
aatctgatac tagtaataaa agatgtttat tttcattaga tgtgtgtgtt ggttttttgt     3780
ctatagcatg c                                                          3791
