                         SEQUENCE LISTING

<110>  LigoCyte Pharmaceuticals, Inc.
       Steadman, Bryan
       Taylor, Ross
 
<120>  TARGETED HETEROLOGOUS ANTIGEN PRESENTATION ON CALICIVIRUS 
       VIRUS-LIKE PARTICLES

<130>  LIGO-023/01WO 306927-2136

<150>  US 61/297,109
<151>  2010-01-21

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  530
<212>  PRT
<213>  Norovirus Norwalk virus sp.

<400>  1

Met Met Met Ala Ser Lys Asp Ala Thr Ser Ser Val Asp Gly Ala Ser 
1               5                   10                  15      


Gly Ala Gly Gln Leu Val Pro Glu Val Asn Ala Ser Asp Pro Leu Ala 
            20                  25                  30          


Met Asp Pro Val Ala Gly Ser Ser Thr Ala Val Ala Thr Ala Gly Gln 
        35                  40                  45              


Val Asn Pro Ile Asp Pro Trp Ile Ile Asn Asn Phe Val Gln Ala Pro 
    50                  55                  60                  


Gln Gly Glu Phe Thr Ile Ser Pro Asn Asn Thr Pro Gly Asp Val Leu 
65                  70                  75                  80  


Phe Asp Leu Ser Leu Gly Pro His Leu Asn Pro Phe Leu Leu His Leu 
                85                  90                  95      


Ser Gln Met Tyr Asn Gly Trp Val Gly Asn Met Arg Val Arg Ile Met 
            100                 105                 110         


Leu Ala Gly Asn Ala Phe Thr Ala Gly Lys Ile Ile Val Ser Cys Ile 
        115                 120                 125             


Pro Pro Gly Phe Gly Ser His Asn Leu Thr Ile Ala Gln Ala Thr Leu 
    130                 135                 140                 


Phe Pro His Val Ile Ala Asp Val Arg Thr Leu Asp Pro Ile Glu Val 
145                 150                 155                 160 


Pro Leu Glu Asp Val Arg Asn Val Leu Phe His Asn Asn Asp Arg Asn 
                165                 170                 175     


Gln Gln Thr Met Arg Leu Val Cys Met Leu Tyr Thr Pro Leu Arg Thr 
            180                 185                 190         


Gly Gly Gly Thr Gly Asp Ser Phe Val Val Ala Gly Arg Val Met Thr 
        195                 200                 205             


Cys Pro Ser Pro Asp Phe Asn Phe Leu Phe Leu Val Pro Pro Thr Val 
    210                 215                 220                 


Glu Gln Lys Thr Arg Pro Phe Thr Leu Pro Asn Leu Pro Leu Ser Ser 
225                 230                 235                 240 


Leu Ser Asn Ser Arg Ala Pro Leu Pro Ile Ser Ser Met Gly Ile Ser 
                245                 250                 255     


Pro Asp Asn Val Gln Ser Val Gln Phe Gln Asn Gly Arg Cys Thr Leu 
            260                 265                 270         


Asp Gly Arg Leu Val Gly Thr Thr Pro Val Ser Leu Ser His Val Ala 
        275                 280                 285             


Lys Ile Arg Gly Thr Ser Asn Gly Thr Val Ile Asn Leu Thr Glu Leu 
    290                 295                 300                 


Asp Gly Thr Pro Phe His Pro Phe Glu Gly Pro Ala Pro Ile Gly Phe 
305                 310                 315                 320 


Pro Asp Leu Gly Gly Cys Asp Trp His Ile Asn Met Thr Gln Phe Gly 
                325                 330                 335     


His Ser Ser Gln Thr Gln Tyr Asp Val Asp Thr Thr Pro Asp Thr Phe 
            340                 345                 350         


Val Pro His Leu Gly Ser Ile Gln Ala Asn Gly Ile Gly Ser Gly Asn 
        355                 360                 365             


Tyr Val Gly Val Leu Ser Trp Ile Ser Pro Pro Ser His Pro Ser Gly 
    370                 375                 380                 


Ser Gln Val Asp Leu Trp Lys Ile Pro Asn Tyr Gly Ser Ser Ile Thr 
385                 390                 395                 400 


Glu Ala Thr His Leu Ala Pro Ser Val Tyr Pro Pro Gly Phe Gly Glu 
                405                 410                 415     


Val Leu Val Phe Phe Met Ser Lys Met Pro Gly Pro Gly Ala Tyr Asn 
            420                 425                 430         


Leu Pro Cys Leu Leu Pro Gln Glu Tyr Ile Ser His Leu Ala Ser Glu 
        435                 440                 445             


Gln Ala Pro Thr Val Gly Glu Ala Ala Leu Leu His Tyr Val Asp Pro 
    450                 455                 460                 


Asp Thr Gly Arg Asn Leu Gly Glu Phe Lys Ala Tyr Pro Asp Gly Phe 
465                 470                 475                 480 


Leu Thr Cys Val Pro Asn Gly Ala Ser Ser Gly Pro Gln Gln Leu Pro 
                485                 490                 495     


Ile Asn Gly Val Phe Val Phe Val Ser Trp Val Ser Arg Phe Tyr Gln 
            500                 505                 510         


Leu Lys Pro Val Gly Thr Ala Ser Ser Ala Arg Gly Arg Leu Gly Leu 
        515                 520                 525             


Arg Arg 
    530 


<210>  2
<211>  540
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Composite GII.4 Norovirus VP1 amino acid sequence

<400>  2

Met Lys Met Ala Ser Ser Asp Ala Asn Pro Ser Asp Gly Ser Thr Ala 
1               5                   10                  15      


Asn Leu Val Pro Glu Val Asn Asn Glu Val Met Ala Leu Glu Pro Val 
            20                  25                  30          


Val Gly Ala Ala Ile Ala Ala Pro Val Ala Gly Gln Gln Asn Val Ile 
        35                  40                  45              


Asp Pro Trp Ile Arg Asn Asn Phe Val Gln Ala Pro Gly Gly Glu Phe 
    50                  55                  60                  


Thr Val Ser Pro Arg Asn Ala Pro Gly Glu Ile Leu Trp Ser Ala Pro 
65                  70                  75                  80  


Leu Gly Pro Asp Leu Asn Pro Tyr Leu Ser His Leu Ala Arg Met Tyr 
                85                  90                  95      


Asn Gly Tyr Ala Gly Gly Phe Glu Val Gln Val Ile Leu Ala Gly Asn 
            100                 105                 110         


Ala Phe Thr Ala Gly Lys Ile Ile Phe Ala Ala Val Pro Pro Asn Phe 
        115                 120                 125             


Pro Thr Glu Gly Leu Ser Pro Ser Gln Val Thr Met Phe Pro His Ile 
    130                 135                 140                 


Ile Val Asp Val Arg Gln Leu Glu Pro Val Leu Ile Pro Leu Pro Asp 
145                 150                 155                 160 


Val Arg Asn Asn Phe Tyr His Tyr Asn Gln Ser Asn Asp Pro Thr Ile 
                165                 170                 175     


Lys Leu Ile Ala Met Leu Tyr Thr Pro Leu Arg Ala Asn Asn Ala Gly 
            180                 185                 190         


Asp Asp Val Phe Thr Val Ser Cys Arg Val Leu Thr Arg Pro Ser Pro 
        195                 200                 205             


Asp Phe Asp Phe Ile Phe Leu Val Pro Pro Thr Val Glu Ser Arg Thr 
    210                 215                 220                 


Lys Pro Phe Thr Val Pro Ile Leu Thr Val Glu Glu Met Thr Asn Ser 
225                 230                 235                 240 


Arg Phe Pro Ile Pro Leu Glu Lys Leu Phe Thr Gly Pro Ser Gly Ala 
                245                 250                 255     


Phe Val Val Gln Pro Gln Asn Gly Arg Cys Thr Thr Asp Gly Val Leu 
            260                 265                 270         


Leu Gly Thr Thr Gln Leu Ser Pro Val Asn Ile Cys Thr Phe Arg Gly 
        275                 280                 285             


Asp Val Thr His Ile Ala Gly Thr Gln Glu Tyr Thr Met Asn Leu Ala 
    290                 295                 300                 


Ser Gln Asn Trp Asn Asn Tyr Asp Pro Thr Glu Glu Ile Pro Ala Pro 
305                 310                 315                 320 


Leu Gly Thr Pro Asp Phe Val Gly Lys Ile Gln Gly Val Leu Thr Gln 
                325                 330                 335     


Thr Thr Arg Gly Asp Gly Ser Thr Arg Gly His Lys Ala Thr Val Ser 
            340                 345                 350         


Thr Gly Ser Val His Phe Thr Pro Lys Leu Gly Ser Val Gln Phe Ser 
        355                 360                 365             


Thr Asp Thr Ser Asn Asp Phe Glu Thr Gly Gln Asn Thr Lys Phe Thr 
    370                 375                 380                 


Pro Val Gly Val Val Gln Asp Gly Ser Thr Thr His Gln Asn Glu Pro 
385                 390                 395                 400 


Gln Gln Trp Val Leu Pro Asp Tyr Ser Gly Arg Asp Ser His Asn Val 
                405                 410                 415     


His Leu Ala Pro Ala Val Ala Pro Thr Phe Pro Gly Glu Gln Leu Leu 
            420                 425                 430         


Phe Phe Arg Ser Thr Met Pro Gly Cys Ser Gly Tyr Pro Asn Met Asn 
        435                 440                 445             


Leu Asp Cys Leu Leu Pro Gln Glu Trp Val Gln His Phe Tyr Gln Glu 
    450                 455                 460                 


Ala Ala Pro Ala Gln Ser Asp Val Ala Leu Leu Arg Phe Val Asn Pro 
465                 470                 475                 480 


Asp Thr Gly Arg Val Leu Phe Glu Cys Lys Leu His Lys Ser Gly Tyr 
                485                 490                 495     


Val Thr Val Ala His Thr Gly Gln His Asp Leu Val Ile Pro Pro Asn 
            500                 505                 510         


Gly Tyr Phe Arg Phe Asp Ser Trp Val Asn Gln Phe Tyr Thr Leu Ala 
        515                 520                 525             


Pro Met Gly Asn Gly Thr Gly Arg Arg Arg Ala Leu 
    530                 535                 540 


<210>  3
<211>  551
<212>  PRT
<213>  Sapovirus sp.

<400>  3

Met Glu Gly Asn Gly Leu Pro Gln Ala Gly Gln Gln Gln Ala Leu Asp 
1               5                   10                  15      


Val Pro Gly Thr Thr Gly Pro Thr Ser Ser Ala Val Val Val Ala Asn 
            20                  25                  30          


Pro Asp Gln Pro Ser Ala Gln Ala Gln Arg Met Glu Leu Ala Val Ala 
        35                  40                  45              


Thr Gly Ala Val Ser Ser Asn Val Pro Asp Ala Val Arg Gln Cys Phe 
    50                  55                  60                  


Ala Leu Leu Arg Thr Phe Pro Trp Asn Thr Arg Gln Ala Thr Gly Thr 
65                  70                  75                  80  


Tyr Leu Gly Ser Ala Ala Leu Ser Pro Ala Leu Asn Pro Tyr Thr Ala 
                85                  90                  95      


His Leu Ser Ala Met Trp Ala Gly Trp Gly Gly Ser Met Glu Ala Arg 
            100                 105                 110         


Val Thr Ile Ser Gly Ser Gly Thr Phe Ala Gly Lys Ile Ile Val Ala 
        115                 120                 125             


Leu Leu Pro Pro Gly Leu Asp Pro Thr Arg Val Arg Asp Pro Gly Val 
    130                 135                 140                 


Leu Pro His Ala Gln Val Asp Ala Arg Ala Val Asp Pro Ile Thr Phe 
145                 150                 155                 160 


Asn Ile Asn Asp Val Arg Ala Val Asp Tyr His Arg Thr Asp Gly Gln 
                165                 170                 175     


Glu Ala Thr Ser Thr Leu Gly Phe Trp Val Leu Gln Pro Leu Ile Asn 
            180                 185                 190         


Pro Phe Ser His Asp Ala Leu Ser Thr Ala Trp Val Ser Val Glu Thr 
        195                 200                 205             


Arg Pro Gly Pro Asp Phe Asp Phe Cys Leu Leu Lys Pro Pro Gln Met 
    210                 215                 220                 


Glu Met Glu Asn Gly Leu Ser Pro Ser Thr Leu Leu Pro Arg His Leu 
225                 230                 235                 240 


Gly Arg Ser Arg Gly Asn Arg Cys Gly Gly Phe Ile Val Gly Met Ala 
                245                 250                 255     


Val Val Ala Met Ala His Gln Val Asn His His Phe Ser Thr Ala Ala 
            260                 265                 270         


Thr Thr Tyr Gly Trp Ser Thr Leu Pro Leu Gly Pro Cys Ala Ala Lys 
        275                 280                 285             


Ile Thr Ser Ser Leu Pro Gly Glu Ile Asn Asn Tyr Thr Gly Phe Ala 
    290                 295                 300                 


Asp Val Asp Gly Ala Gly Glu Gly Pro Ile Met Pro Asn Ile Pro Asn 
305                 310                 315                 320 


His Trp Pro Asp Ser Cys Ala Ser Ser Val Ile Ala Thr Trp Asp Ser 
                325                 330                 335     


Ser Leu His Arg Pro Asn Leu Gly Ile Ser Gly Ser Ile Met Thr Phe 
            340                 345                 350         


Asp Asn His Gly Asp Ala Asp Glu Ala Gln Ile Thr Gly Ala Met Ala 
        355                 360                 365             


Ala Thr Val Asp Pro Ser Pro Ser Arg Arg Thr Gln Leu Gln Gly Ser 
    370                 375                 380                 


Phe Thr Ala Asn Thr Met Arg Ile Met Arg Thr Ser Gly Leu Asp Lys 
385                 390                 395                 400 


Ile Gly Glu Val Asn Lys Asn Val Tyr Phe Ile Pro Ile Leu Leu Asp 
                405                 410                 415     


Gly Ala Thr Gly Tyr Ile Asn Glu Lys Val Thr Asn Leu Ala Asp Ile 
            420                 425                 430         


Asn Ile Ser Tyr Gly Pro Val Gly Ser Asn Asn Val Ile Leu Trp Arg 
        435                 440                 445             


Glu Arg Val Phe Ser Ser His Pro Arg Pro Gly Ile Leu Tyr Ser Ser 
    450                 455                 460                 


Gln Leu Glu Ser Thr Ala Ser Ile Phe Gln Asp Gly Pro Val Asn Ile 
465                 470                 475                 480 


Pro Asn Asn Tyr Met Ala Val Phe Asn Val Ser Asp Thr Gly Ala Asp 
                485                 490                 495     


Phe Gln Ile Gly Ile Cys Pro Asp Gly Tyr Met Arg Thr Gly Ser Pro 
            500                 505                 510         


Val Gly Thr Val Val Asp Leu Thr Pro Glu Cys Thr Phe Thr Phe Val 
        515                 520                 525             


Gly Leu Phe Pro Phe Thr Ser Pro Leu Asn Gly Pro His Gly Thr Gly 
    530                 535                 540                 


Arg Gly Arg Ser Val Tyr Gln 
545                 550     


<210>  4
<211>  558
<212>  PRT
<213>  Vesivirus San Miguel sea lion sp.

<400>  4

Ser Asp Gly Pro Gly Ser Ala Glu Ile Val Thr Glu Glu Gln Gly Thr 
1               5                   10                  15      


Val Val Gln Gln Gln Pro Ala Pro Ala Pro Thr Ala Leu Ala Thr Leu 
            20                  25                  30          


Ala Thr Ala Ser Thr Gly Lys Ser Val Glu Gln Glu Trp Met Thr Phe 
        35                  40                  45              


Phe Ser Tyr His Thr Ser Ile Asn Trp Ser Thr Val Glu Ser Gln Gly 
    50                  55                  60                  


Lys Ile Leu Tyr Ser Gln Ala Leu Asn Pro Ser Ile Asn Pro Tyr Leu 
65                  70                  75                  80  


Asp His Ile Ala Lys Leu Tyr Ser Thr Trp Ser Gly Gly Ile Asp Val 
                85                  90                  95      


Arg Phe Thr Val Ser Gly Ser Gly Val Phe Gly Gly Lys Leu Ala Ala 
            100                 105                 110         


Leu Leu Val Pro Pro Gly Val Glu Pro Ile Glu Ser Val Ser Met Leu 
        115                 120                 125             


Gln Tyr Pro His Val Leu Phe Asp Ala Arg Gln Thr Glu Pro Val Ile 
    130                 135                 140                 


Phe Thr Ile Pro Asp Ile Arg Lys Thr Leu Phe His Ser Met Asp Glu 
145                 150                 155                 160 


Thr Asp Thr Thr Lys Leu Val Ile Met Val Tyr Asn Glu Leu Ile Asn 
                165                 170                 175     


Pro Tyr Glu Asn Gly Val Glu Asn Lys Thr Thr Cys Ser Ile Thr Val 
            180                 185                 190         


Glu Thr Arg Pro Ser Ala Asp Phe Thr Phe Ala Leu Leu Lys Pro Pro 
        195                 200                 205             


Gly Ser Leu Ile Lys His Gly Ser Ile Pro Ser Asp Leu Ile Pro Arg 
    210                 215                 220                 


Asn Ser Ala His Trp Met Gly Asn Arg Trp Trp Ser Thr Ile Ser Gly 
225                 230                 235                 240 


Phe Ser Val Gln Pro Arg Val Phe Gln Ser Asn Arg His Phe Asp Phe 
                245                 250                 255     


Asp Ser Thr Thr Thr Gly Trp Ser Thr Pro Tyr Tyr Val Pro Ile Glu 
            260                 265                 270         


Ile Lys Ile Gln Gly Lys Val Gly Ser Asn Asn Lys Trp Phe His Val 
        275                 280                 285             


Ile Asp Thr Asp Lys Ala Leu Val Pro Gly Ile Pro Asp Gly Trp Pro 
    290                 295                 300                 


Asp Thr Thr Ile Pro Asp Glu Thr Lys Ala Thr Asn Gly Asn Phe Ser 
305                 310                 315                 320 


Tyr Gly Glu Ser Tyr Arg Ala Gly Ser Thr Thr Ile Lys Pro Asn Glu 
                325                 330                 335     


Asn Ser Thr His Phe Lys Gly Thr Tyr Ile Cys Gly Thr Leu Ser Thr 
            340                 345                 350         


Val Glu Ile Pro Glu Asn Asp Glu Gln Gln Ile Lys Thr Glu Ala Glu 
        355                 360                 365             


Lys Lys Ser Gln Thr Met Tyr Val Val Thr Ala Asp Phe Lys Asp Thr 
    370                 375                 380                 


Ile Val Lys Pro Gln His Lys Ile Ser Pro Gln Lys Leu Val Val Tyr 
385                 390                 395                 400 


Phe Asp Gly Pro Glu Lys Asp Leu Thr Met Ser Ala Thr Leu Ser Pro 
                405                 410                 415     


Leu Gly Tyr Thr Leu Val Asp Glu Gln Pro Val Gly Ser Val Ser Ser 
            420                 425                 430         


Arg Val Val Arg Ile Ala Thr Leu Pro Glu Ala Phe Thr Gln Gly Gly 
        435                 440                 445             


Asn Tyr Pro Ile Phe Tyr Val Asn Lys Ile Lys Val Gly Tyr Phe Asp 
    450                 455                 460                 


Arg Ala Thr Thr Asn Cys Tyr Asn Ser Gln Ile Leu Met Thr Ser Gln 
465                 470                 475                 480 


Arg Leu Ala Glu Gly Asn Tyr Asn Leu Pro Pro Asp Ser Leu Ala Val 
                485                 490                 495     


Tyr Arg Ile Thr Asp Ser Ser Ser Gln Trp Phe Asp Ile Gly Ile Asn 
            500                 505                 510         


His Asp Gly Phe Ser Tyr Val Gly Leu Ser Asp Leu Pro Asn Asp Leu 
        515                 520                 525             


Ser Phe Pro Leu Thr Ser Thr Phe Met Gly Val Gln Leu Ala Arg Val 
    530                 535                 540                 


Lys Leu Ala Ser Lys Val Lys Ala His Thr Ile Thr Ala Lys 
545                 550                 555             


<210>  5
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  influenza hemagglutinin antigen epitope

<400>  5

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Respiratory syncytial virus F protein antigenic epitope

<400>  6

Leu Ile Asn Asp Met Pro Ile Thr Asn 
1               5                   


<210>  7
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Respiratory syncytial virus F protein antigenic epitope

<400>  7

Tyr Met Leu Thr Asn Ser Glu Leu Leu Ser Leu Ile Asn Asp Met Pro 
1               5                   10                  15      


Ile Thr Asn Asp Gln Lys Lys Leu Met Ser Asn Asn Val 
            20                  25                  


