                         SEQUENCE LISTING

<110>  Syngenta Participations AG
       Steffens, John
       Fourage, Laurent
       Oeller, Paul
 
<120>  COMPOSITIONS AND METHODS RELATING TO DUAL ACTIVITY ENZYMES 
       HAVING XYLANASE AND CELLULASE ACTIVITY

<130>  9848-8WO

<150>  US 61/298,020
<151>  2010-01-25

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  1507
<212>  DNA
<213>  Dictyoglomus thermophilum


<220>
<221>  CDS
<222>  (244)..(1302)

<400>  1
aagcttgagg aattgttgtt gggtgatttg aagaaagaag gttatgatgt attttaccaa       60

tatgtctata ttaaaaggaa ttttagattt gcggaaagaa taaagagcct aatgaaagat      120

tacaaatcta tatttgtggt aataggtgca ggtcatttaa taggtgaaga aaacgtggta      180

aaattattac aaaattaggg gttaaagtaa gtaaaatttt aatggaaagg ggatgatggt      240

aaa atg att aac caa agg ttt tct atc ctt gtg ctt ctt cta att ttg        288
    Met Ile Asn Gln Arg Phe Ser Ile Leu Val Leu Leu Leu Ile Leu           
    1               5                   10                  15            

ctc act ttc tca tta gga ttt tta aag gag gag gct aaa ggt atg gag        336
Leu Thr Phe Ser Leu Gly Phe Leu Lys Glu Glu Ala Lys Gly Met Glu           
                20                  25                  30                

att cca tca ctg aaa gaa gtt tat aaa gat tac ttt act att gga gcg        384
Ile Pro Ser Leu Lys Glu Val Tyr Lys Asp Tyr Phe Thr Ile Gly Ala           
            35                  40                  45                    

gca gtt agt cac tta aat att tat cat tat gag aat ctt ttg aag aag        432
Ala Val Ser His Leu Asn Ile Tyr His Tyr Glu Asn Leu Leu Lys Lys           
        50                  55                  60                        

cat ttt aat agt tta act cct gaa aat caa atg aaa tgg gaa gtt att        480
His Phe Asn Ser Leu Thr Pro Glu Asn Gln Met Lys Trp Glu Val Ile           
    65                  70                  75                            

cat cct aaa cca tat gtt tat gat ttt gga cct gcc gat gag atc gta        528
His Pro Lys Pro Tyr Val Tyr Asp Phe Gly Pro Ala Asp Glu Ile Val           
80                  85                  90                  95            

gat ttt gca atg aag aat ggt atg aag gta aga ggg cat act ttg gtt        576
Asp Phe Ala Met Lys Asn Gly Met Lys Val Arg Gly His Thr Leu Val           
                100                 105                 110               

tgg cat aat cag act cca gga tgg gtt tat gca ggt aca aag gat gaa        624
Trp His Asn Gln Thr Pro Gly Trp Val Tyr Ala Gly Thr Lys Asp Glu           
            115                 120                 125                   

att ctt gca aga tta aag gaa cac att aaa gag gta gta gga cat tat        672
Ile Leu Ala Arg Leu Lys Glu His Ile Lys Glu Val Val Gly His Tyr           
        130                 135                 140                       

aaa ggt aaa gtt tat gct tgg gat gtg gtt aac gag gcc tta tcg gat        720
Lys Gly Lys Val Tyr Ala Trp Asp Val Val Asn Glu Ala Leu Ser Asp           
    145                 150                 155                           

aat cct aat gaa ttt ttg aga aga gct cct tgg tat gat att tgt ggt        768
Asn Pro Asn Glu Phe Leu Arg Arg Ala Pro Trp Tyr Asp Ile Cys Gly           
160                 165                 170                 175           

gaa gaa gtt att gaa aag gca ttt att tgg gct cat gag gta gat cct        816
Glu Glu Val Ile Glu Lys Ala Phe Ile Trp Ala His Glu Val Asp Pro           
                180                 185                 190               

gat gct aaa tta ttt tat aac gat tac aat tta gaa gat cct ata aag        864
Asp Ala Lys Leu Phe Tyr Asn Asp Tyr Asn Leu Glu Asp Pro Ile Lys           
            195                 200                 205                   

aga gaa aag gct tat aaa tta gtt aag aag ctc aag gat aaa gga gtt        912
Arg Glu Lys Ala Tyr Lys Leu Val Lys Lys Leu Lys Asp Lys Gly Val           
        210                 215                 220                       

cct att cat ggt att gga ata cag gga cat tgg aca tta gca tgg cca        960
Pro Ile His Gly Ile Gly Ile Gln Gly His Trp Thr Leu Ala Trp Pro           
    225                 230                 235                           

act cca aag atg ctt gag gat tcc ata aag aga ttt gca gag ctt gga       1008
Thr Pro Lys Met Leu Glu Asp Ser Ile Lys Arg Phe Ala Glu Leu Gly           
240                 245                 250                 255           

gtt gaa gta caa gtt aca gag ttt gat att tct att tac tac gat aga       1056
Val Glu Val Gln Val Thr Glu Phe Asp Ile Ser Ile Tyr Tyr Asp Arg           
                260                 265                 270               

aat gaa aat aat aat ttt aag gtt ccg cct gaa gat agg ctt gaa aga       1104
Asn Glu Asn Asn Asn Phe Lys Val Pro Pro Glu Asp Arg Leu Glu Arg           
            275                 280                 285                   

caa gct cag ctt tat aag gag gct ttt gaa atc tta aga aaa tac aaa       1152
Gln Ala Gln Leu Tyr Lys Glu Ala Phe Glu Ile Leu Arg Lys Tyr Lys           
        290                 295                 300                       

gga ata gtt act ggt gtt act ttc tgg ggt gta gca gat gac tat act       1200
Gly Ile Val Thr Gly Val Thr Phe Trp Gly Val Ala Asp Asp Tyr Thr           
    305                 310                 315                           

tgg ctc tat ttc tgg cct gtt aga gga aga gaa gat tat cca tta ctc       1248
Trp Leu Tyr Phe Trp Pro Val Arg Gly Arg Glu Asp Tyr Pro Leu Leu           
320                 325                 330                 335           

ttt gat aag aat cac aat cct aag aag gct ttc tgg gag att gta aag       1296
Phe Asp Lys Asn His Asn Pro Lys Lys Ala Phe Trp Glu Ile Val Lys           
                340                 345                 350               

ttt tag gttgtatagg aggtttaaat gagaaaatta ttaaggtttt tgattttttc        1352
Phe                                                                       
                                                                          

tctattagtt ttaggacttt ctttcaatat tattttagct gagactgaga aatatgtgga     1412

taagaattta ggtttttcta ttgtgcctcc tgaagggtgg gaggtaaagg atggaaagcc     1472

ttataacttg gctgtaattt ttgttggccc tgcag                                1507


<210>  2
<211>  352
<212>  PRT
<213>  Dictyoglomus thermophilum

<400>  2

Met Ile Asn Gln Arg Phe Ser Ile Leu Val Leu Leu Leu Ile Leu Leu 
1               5                   10                  15      


Thr Phe Ser Leu Gly Phe Leu Lys Glu Glu Ala Lys Gly Met Glu Ile 
            20                  25                  30          


Pro Ser Leu Lys Glu Val Tyr Lys Asp Tyr Phe Thr Ile Gly Ala Ala 
        35                  40                  45              


Val Ser His Leu Asn Ile Tyr His Tyr Glu Asn Leu Leu Lys Lys His 
    50                  55                  60                  


Phe Asn Ser Leu Thr Pro Glu Asn Gln Met Lys Trp Glu Val Ile His 
65                  70                  75                  80  


Pro Lys Pro Tyr Val Tyr Asp Phe Gly Pro Ala Asp Glu Ile Val Asp 
                85                  90                  95      


Phe Ala Met Lys Asn Gly Met Lys Val Arg Gly His Thr Leu Val Trp 
            100                 105                 110         


His Asn Gln Thr Pro Gly Trp Val Tyr Ala Gly Thr Lys Asp Glu Ile 
        115                 120                 125             


Leu Ala Arg Leu Lys Glu His Ile Lys Glu Val Val Gly His Tyr Lys 
    130                 135                 140                 


Gly Lys Val Tyr Ala Trp Asp Val Val Asn Glu Ala Leu Ser Asp Asn 
145                 150                 155                 160 


Pro Asn Glu Phe Leu Arg Arg Ala Pro Trp Tyr Asp Ile Cys Gly Glu 
                165                 170                 175     


Glu Val Ile Glu Lys Ala Phe Ile Trp Ala His Glu Val Asp Pro Asp 
            180                 185                 190         


Ala Lys Leu Phe Tyr Asn Asp Tyr Asn Leu Glu Asp Pro Ile Lys Arg 
        195                 200                 205             


Glu Lys Ala Tyr Lys Leu Val Lys Lys Leu Lys Asp Lys Gly Val Pro 
    210                 215                 220                 


Ile His Gly Ile Gly Ile Gln Gly His Trp Thr Leu Ala Trp Pro Thr 
225                 230                 235                 240 


Pro Lys Met Leu Glu Asp Ser Ile Lys Arg Phe Ala Glu Leu Gly Val 
                245                 250                 255     


Glu Val Gln Val Thr Glu Phe Asp Ile Ser Ile Tyr Tyr Asp Arg Asn 
            260                 265                 270         


Glu Asn Asn Asn Phe Lys Val Pro Pro Glu Asp Arg Leu Glu Arg Gln 
        275                 280                 285             


Ala Gln Leu Tyr Lys Glu Ala Phe Glu Ile Leu Arg Lys Tyr Lys Gly 
    290                 295                 300                 


Ile Val Thr Gly Val Thr Phe Trp Gly Val Ala Asp Asp Tyr Thr Trp 
305                 310                 315                 320 


Leu Tyr Phe Trp Pro Val Arg Gly Arg Glu Asp Tyr Pro Leu Leu Phe 
                325                 330                 335     


Asp Lys Asn His Asn Pro Lys Lys Ala Phe Trp Glu Ile Val Lys Phe 
            340                 345                 350         


<210>  3
<211>  999
<212>  DNA
<213>  Artificial

<220>
<223>  Coding sequence for D. thermophilum xylanase without targeting
       sequence


<220>
<221>  CDS
<222>  (1)..(999)

<400>  3
atg ttt ctg aaa gaa gaa gcc aaa ggt atg gaa att ccg agc ctg aaa         48
Met Phe Leu Lys Glu Glu Ala Lys Gly Met Glu Ile Pro Ser Leu Lys           
1               5                   10                  15                

gaa gtg tat aaa gat tac ttt acc atc ggt gca gca gtt agc cat ctg         96
Glu Val Tyr Lys Asp Tyr Phe Thr Ile Gly Ala Ala Val Ser His Leu           
            20                  25                  30                    

aat att tat cat tat gag aac ctg ctg aaa aaa cat ttt aat agc ctg        144
Asn Ile Tyr His Tyr Glu Asn Leu Leu Lys Lys His Phe Asn Ser Leu           
        35                  40                  45                        

aca ccg gaa aat cag atg aaa tgg gaa gtg att cat ccg aaa ccg tat        192
Thr Pro Glu Asn Gln Met Lys Trp Glu Val Ile His Pro Lys Pro Tyr           
    50                  55                  60                            

gtg tat gat ttt ggt ccg gca gat gaa att gtt gat ttt gcc atg aaa        240
Val Tyr Asp Phe Gly Pro Ala Asp Glu Ile Val Asp Phe Ala Met Lys           
65                  70                  75                  80            

aat ggc atg aaa gtt cgt ggt cat acc ctg gtt tgg cat aat cag aca        288
Asn Gly Met Lys Val Arg Gly His Thr Leu Val Trp His Asn Gln Thr           
                85                  90                  95                

ccg ggt tgg gtt tat gca ggc acc aaa gat gaa att ctg gca cgc ctg        336
Pro Gly Trp Val Tyr Ala Gly Thr Lys Asp Glu Ile Leu Ala Arg Leu           
            100                 105                 110                   

aaa gaa cat att aaa gaa gtg gtg ggc cat tat aaa ggt aaa gtg tat        384
Lys Glu His Ile Lys Glu Val Val Gly His Tyr Lys Gly Lys Val Tyr           
        115                 120                 125                       

gcc tgg gat gtt gtt aat gaa gcc ctg agc gat aat ccg aat gaa ttt        432
Ala Trp Asp Val Val Asn Glu Ala Leu Ser Asp Asn Pro Asn Glu Phe           
    130                 135                 140                           

ctg cgt cgt gca ccg tgg tat gat att tgt ggc gaa gaa gtg att gaa        480
Leu Arg Arg Ala Pro Trp Tyr Asp Ile Cys Gly Glu Glu Val Ile Glu           
145                 150                 155                 160           

aaa gcc ttt att tgg gca cat gaa gtt gat ccg gat gcc aaa ctg ttt        528
Lys Ala Phe Ile Trp Ala His Glu Val Asp Pro Asp Ala Lys Leu Phe           
                165                 170                 175               

tat aat gat tat aat ctg gaa gat ccg att aaa cgc gaa aaa gcc tat        576
Tyr Asn Asp Tyr Asn Leu Glu Asp Pro Ile Lys Arg Glu Lys Ala Tyr           
            180                 185                 190                   

aaa ctg gtg aaa aaa ctg aaa gat aaa ggc gtt ccg att cat ggt att        624
Lys Leu Val Lys Lys Leu Lys Asp Lys Gly Val Pro Ile His Gly Ile           
        195                 200                 205                       

ggt att cag ggt cat tgg acc ctg gca tgg ccg acc ccg aaa atg ctg        672
Gly Ile Gln Gly His Trp Thr Leu Ala Trp Pro Thr Pro Lys Met Leu           
    210                 215                 220                           

gaa gat agc att aaa cgt ttt gcc gaa ctg ggt gtt gaa gtt cag gtg        720
Glu Asp Ser Ile Lys Arg Phe Ala Glu Leu Gly Val Glu Val Gln Val           
225                 230                 235                 240           

acc gag ttc gac atc agc atc tac tat gat cgc aat gaa aac aat aac        768
Thr Glu Phe Asp Ile Ser Ile Tyr Tyr Asp Arg Asn Glu Asn Asn Asn           
                245                 250                 255               

ttt aaa gtg cct ccg gaa gat cgt ctg gaa cgt cag gca cag ctg tat        816
Phe Lys Val Pro Pro Glu Asp Arg Leu Glu Arg Gln Ala Gln Leu Tyr           
            260                 265                 270                   

aaa gaa gcc ttt gaa att ctg cgc aaa tac aaa ggc atc gtg acc ggc        864
Lys Glu Ala Phe Glu Ile Leu Arg Lys Tyr Lys Gly Ile Val Thr Gly           
        275                 280                 285                       

gtg acc ttt tgg ggt gtt gca gat gat tat acc tgg ctg tat ttt tgg        912
Val Thr Phe Trp Gly Val Ala Asp Asp Tyr Thr Trp Leu Tyr Phe Trp           
    290                 295                 300                           

ccg gtt cgt ggt cgt gaa gat tat ccg ctg ctg ttt gat aaa aat cat        960
Pro Val Arg Gly Arg Glu Asp Tyr Pro Leu Leu Phe Asp Lys Asn His           
305                 310                 315                 320           

aat ccg aaa aaa gcc ttt tgg gaa att gtg aaa ttt taa                    999
Asn Pro Lys Lys Ala Phe Trp Glu Ile Val Lys Phe                           
                325                 330                                   


<210>  4
<211>  332
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  4

Met Phe Leu Lys Glu Glu Ala Lys Gly Met Glu Ile Pro Ser Leu Lys 
1               5                   10                  15      


Glu Val Tyr Lys Asp Tyr Phe Thr Ile Gly Ala Ala Val Ser His Leu 
            20                  25                  30          


Asn Ile Tyr His Tyr Glu Asn Leu Leu Lys Lys His Phe Asn Ser Leu 
        35                  40                  45              


Thr Pro Glu Asn Gln Met Lys Trp Glu Val Ile His Pro Lys Pro Tyr 
    50                  55                  60                  


Val Tyr Asp Phe Gly Pro Ala Asp Glu Ile Val Asp Phe Ala Met Lys 
65                  70                  75                  80  


Asn Gly Met Lys Val Arg Gly His Thr Leu Val Trp His Asn Gln Thr 
                85                  90                  95      


Pro Gly Trp Val Tyr Ala Gly Thr Lys Asp Glu Ile Leu Ala Arg Leu 
            100                 105                 110         


Lys Glu His Ile Lys Glu Val Val Gly His Tyr Lys Gly Lys Val Tyr 
        115                 120                 125             


Ala Trp Asp Val Val Asn Glu Ala Leu Ser Asp Asn Pro Asn Glu Phe 
    130                 135                 140                 


Leu Arg Arg Ala Pro Trp Tyr Asp Ile Cys Gly Glu Glu Val Ile Glu 
145                 150                 155                 160 


Lys Ala Phe Ile Trp Ala His Glu Val Asp Pro Asp Ala Lys Leu Phe 
                165                 170                 175     


Tyr Asn Asp Tyr Asn Leu Glu Asp Pro Ile Lys Arg Glu Lys Ala Tyr 
            180                 185                 190         


Lys Leu Val Lys Lys Leu Lys Asp Lys Gly Val Pro Ile His Gly Ile 
        195                 200                 205             


Gly Ile Gln Gly His Trp Thr Leu Ala Trp Pro Thr Pro Lys Met Leu 
    210                 215                 220                 


Glu Asp Ser Ile Lys Arg Phe Ala Glu Leu Gly Val Glu Val Gln Val 
225                 230                 235                 240 


Thr Glu Phe Asp Ile Ser Ile Tyr Tyr Asp Arg Asn Glu Asn Asn Asn 
                245                 250                 255     


Phe Lys Val Pro Pro Glu Asp Arg Leu Glu Arg Gln Ala Gln Leu Tyr 
            260                 265                 270         


Lys Glu Ala Phe Glu Ile Leu Arg Lys Tyr Lys Gly Ile Val Thr Gly 
        275                 280                 285             


Val Thr Phe Trp Gly Val Ala Asp Asp Tyr Thr Trp Leu Tyr Phe Trp 
    290                 295                 300                 


Pro Val Arg Gly Arg Glu Asp Tyr Pro Leu Leu Phe Asp Lys Asn His 
305                 310                 315                 320 


Asn Pro Lys Lys Ala Phe Trp Glu Ile Val Lys Phe 
                325                 330         


<210>  5
<211>  999
<212>  DNA
<213>  Artificial

<220>
<223>  Coding sequence for D. thermophilum xylanase without targeting
       sequence

<400>  5
atgtttttaa aggaggaggc taaaggtatg gagattccat cactgaaaga agtttataaa       60

gattacttta ctattggagc ggcagttagt cacttaaata tttatcatta tgagaatctt      120

ttgaagaagc attttaatag tttaactcct gaaaatcaaa tgaaatggga agttattcat      180

cctaaaccat atgtttatga ttttggacct gccgatgaga tcgtagattt tgcaatgaag      240

aatggtatga aggtaagagg gcatactttg gtttggcata atcagactcc aggatgggtt      300

tatgcaggta caaaggatga aattcttgca agattaaagg aacacattaa agaggtagta      360

ggacattata aaggtaaagt ttatgcttgg gatgtggtta acgaggcctt atcggataat      420

cctaatgaat ttttgagaag agctccttgg tatgatattt gtggtgaaga agttattgaa      480

aaggcattta tttgggctca tgaggtagat cctgatgcta aattatttta taacgattac      540

aatttagaag atcctataaa gagagaaaag gcttataaat tagttaagaa gctcaaggat      600

aaaggagttc ctattcatgg tattggaata cagggacatt ggacattagc atggccaact      660

ccaaagatgc ttgaggattc cataaagaga tttgcagagc ttggagttga agtacaagtt      720

acagagtttg atatttctat ttactacgat agaaatgaaa ataataattt taaggttccg      780

cctgaagata ggcttgaaag acaagctcag ctttataagg aggcttttga aatcttaaga      840

aaatacaaag gaatagttac tggtgttact ttctggggtg tagcagatga ctatacttgg      900

ctctatttct ggcctgttag aggaagagaa gattatccat tactctttga taagaatcac      960

aatcctaaga aggctttctg ggagattgta aagttttag                             999


