
                                SEQUENCE LISTING

<110> Oxyrane UK Limited

<120> METHODS AND COMPOSITIONS FOR DISPLAYING
  A POLYPEPTIDE ON A YEAST CELL SURFACE
  

<130> 18990/0024WO1

<150> 61/297,093          
<151> 2010-01-21  

<160> 15

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 1199
<212> DNA
<213> Artificial Sequence

<220> 
<223> Yarrowia lipolytica codon optimized sequence

<400> 1
gaatgcagcg gcccagccgg ccatggccca ggtgcagctg caggtcgacc tcgagtggcg 60
gcggaggctc tggcggaggc ggatctggcg gcggtggcag tgcacaggtc caactgcagg 120
agctcgatat caaacgggcg gccgcagagc agaagctgat ctctgaggaa gatctgtccg 180
gcggaggcgg ctccggtggc ggcggttctg gcggtggcgg ctctcatatg tctgccaagt 240
cctctttcat ctctaccacc accaccgacc tgacctctat caacacctct gcctactcta 300
ccggctctat ctctaccgtg gagaccggca accgaaccac ctctgaagtg atctctcacg 360
tggtgaccac ttctaccaag ctgtctccca ccgccaccac ctccctgacc attgcccaga 420
cctctatcta ctccaccgac tccaacatca ccgtgggcac cgacatccac accacctccg 480
aggtcatttc cgacgtggag accatctccc gagagaccgc ctctaccgtg gtggccgctc 540
ctacctctac caccggctgg accggcgcca tgaacaccta catctctcag ttcacctctt 600
cttccttcgc caccatcaac tctaccccca tcatctcttc ctctgccgtg ttcgagacct 660
ctgacgcctc tatcgtgaac gtccacaccg agaacattac caacaccgcc gctgttccct 720
ctgaggaacc cacctttgtg aacgccaccc gaaactccct gaactctttc tgttcttcta 780
agcagccctc ctctccctct tcctacacct cttcccccct ggtgtcctct ctgtctgtgt 840
ctaagaccct gctgtctacc tctttcaccc cctctgtgcc cacctctaac acctacatta 900
agaccaagaa caccggctac ttcgagcaca ccgccctgac cacctcttct gtgggcctga 960
actccttctc tgagaccgcc gtgtcctctc agggcaccaa gatcgacacc tttctggtct 1020
cctccctgat cgcctacccc tcttctgcct ctggctctca gctgtctggc atccagcaga 1080
acttcacctc tacctccctg atgatctcta cctacgaggg caaggcctct atcttcttct 1140
ctgccgagct gggctctatc atcttcctgc tgctgtctta cctgctgttc taacctagg  1199

<210> 2
<211> 446
<212> DNA
<213> Artificial Sequence

<220> 
<223> Yarrowia lipolytica codon optimized sequence

<400> 2
gaatgcagcg gcccagccgg ccatggccca ggtgcagctg caggtcgacc tcgagtggcg 60
gcggaggctc tggcggaggc ggatctggcg gcggtggcag tgcacaggtc caactgcagg 120
agctcgatat caaacgggcg gccgcagagc agaagctgat ctctgaggaa gatctgtccg 180
gcggaggcgg ctccggtggc ggcggttctg gcggtggcgg ctctcatatg caggaactga 240
ccaccatctg cgagcagatt ccctctccca ccctggagtc taccccctac tctctgtcta 300
ccaccaccat cctggccaac ggcaaggcca tgcagggcgt gttcgagtac tacaagtctg 360
tgaccttcgt gtctaactgt ggctctcacc cctctaccac ctctaagggc tctcccatca 420
acacccagta cgtgttctaa cctagg                                      446

<210> 3
<211> 569
<212> DNA
<213> Artificial Sequence

<220> 
<223> Yarrowia lipolytica codon optimized sequence

<400> 3
gaatgcagcg gcccagccgg ccatggccca ggtgcagctg caggtcgacc tcgagtggcg 60
gcggaggctc tggcggaggc ggatctggcg gcggtggcag tgcacaggtc caactgcagg 120
agctcgatat caaacgggcg gccgcagagc agaagctgat ctctgaggaa gatctgtccg 180
gcggaggcgg ctccggtggc ggcggttctg gcggtggcgg ctctcatatg ggcaacggtt 240
acgccgtcga cgacaactcc aagtgcgagg acgacggaat ccccttcggc gcctacgctg 300
ttgctgacac ctccgcagag tcttctgccg cccccgcctc ttctgccgcc gctgccgagt 360
cctctgccgc cccctcttcc gctgctgagg ccaagcccac cgctggaggt aacaccggcg 420
ccgtcgtcac ccagatcggt gacggccaga tccaggctcc cccctctgct cctcccgctg 480
cccccgagca ggccaacggc gccgtctctg tcggtgtttc tgccgccgct ctcggtgtcg 540
ctgccgccgc tctcctcatt taacctagg                                   569

<210> 4
<211> 470
<212> DNA
<213> Artificial Sequence

<220> 
<223> Yarrowia lipolytica codon optimized sequence

<400> 4
gaatgcacag gaactgacca ccatctgcga gcagattccc tctcccaccc tggagtctac 60
cccctactct ctgtctacca ccaccatcct ggccaacggc aaggccatgc agggcgtgtt 120
cgagtactac aagtctgtga ccttcgtgtc taactgtggc tctcacccct ctaccacctc 180
taagggctct cccatcaaca cccagtacgt gttctcttct ggcggcggag gctctggcgg 240
aggcggatct ggtggcggag gatctgcggc ccagccggcc atggcccagg tgcagctgca 300
ggtcgacctc gagtggaggc ggcggatctg gcggtggcgg ctccggcggt ggaggcagtg 360
cacaggtcca actgcaggag ctcgatatca aacgggcggc cgcagagcag aagctgatct 420
ctgaggaaga tctgcgaacc ggccaccacc accaccacca ctaacctagg            470

<210> 5
<211> 747
<212> DNA
<213> Artificial Sequence

<220> 
<223> Yarrowia lipolytica codon optimized sequence

<400> 5
ggcccagccg gccgaggtgc agctggtcga gtctggcggc ggactggtgc agcccggtgg 60
ctctctgcga ctgtcttgtg ccgcctctgg cttcaacatc aaggacacct acatccactg 120
ggtgcgacag gctcccggaa agggcctgga gtgggtggcc cgaatctacc ccaccaacgg 180
ctacacccga tacgccgact ctgtgaaggg ccgattcacc atctctgccg acacctctaa 240
gaacaccgcc tacctgcaga tgaactctct gcgagccgag gacaccgctg tgtactactg 300
ttctcgatgg ggaggcgacg gcttctacgc catggactac tggggccagg gcaccctggt 360
gaccgtgtcc tctggcggag gcggctccgg cggaggcgga tctggtggcg gaggctctga 420
catccagatg acccagtctc cctcttctct gtctgcctct gtgggcgacc gagtgaccat 480
cacctgtcga gcctctcagg acgtgaacac cgccgtggcc tggtatcagc agaagcccgg 540
caaggccccc aagctgctga tctactctgc ctctttcctg tactctggcg tgccctctcg 600
attctctggc tctcgatctg gcaccgactt caccctgacc atctcttctc tgcagcctga 660
ggatttcgcc acctactact gtcagcagca ctacaccacc ccccccacct tcggccaggg 720
aaccaaggtg gagatcaagg cggccgc                                     747

<210> 6
<211> 756
<212> DNA
<213> Artificial Sequence

<220> 
<223> Yarrowia lipolytica codon optimized sequence

<400> 6
ggcccagccg gccgacgtga agctggacga gactggagga ggcctggtgc agcccggacg 60
acccatgaag ctgtcttgtg tggcctctgg cttcaccttc tctgactact ggatgaactg 120
ggtgcgacag tctcccgaga agggcctgga gtgggtggcc cagatccgaa acaagcccta 180
caactacgag acctactact ctgactctgt gaagggccga ttcaccatgt cccgagatga 240
ctctaagtcc tctgtgtacc tgcagatgaa caacctgcga gtggaggaca tgggcatcta 300
ctactgtacc ggctcttact acggcatgga ctactggggc cagggcacct ctgtgaccgt 360
gtcctctggc ggcggaggct ctggcggagg cggatctggt ggcggaggat ctgacgtggt 420
gatgacccag acccccctgt ctctgcccgt gtctctgggc gaccaggcct ctatctcttg 480
tcgatcttct cagtctctgg tccactctaa cggcaacacc tacctgcgat ggtatctgca 540
gaagcccggc cagtctccca aggtgctgat ctacaaggtg tctaaccgat tctctggcgt 600
gcccgaccga ttctccggct ctggctctgg caccgacttc accctgaaga tctcccgagt 660
ggaggccgag gacctgggcg tgtacttctg ttctcagtct acccacgtgc cctggacctt 720
cggcggaggc accaagctgg agatcaaggc ggccgc                           756

<210> 7
<211> 738
<212> DNA
<213> Artificial Sequence

<220> 
<223> Yarrowia lipolytica codon optimized sequence

<400> 7
ggcccagccg gcccaggtgc agctgcagga atctggcccc ggactggtgg ccccctctca 60
gtctctgtct atcacctgta ccgtgtctgg cttctctctg accggctacg gcgtgaactg 120
ggtgcgacag ccccctggca agggcctgga gtggctgggc atgatctggg gcgacggcaa 180
caccgactac aactctgccc tgaagtctcg actgtctatc tctaaggaca actctaagtc 240
tcaggtgttc ctcaagatga actctctcca caccgacgac accgcccgat actactgtgc 300
ccgagagcga gactaccgac tggactactg gggccagggc accaccgtga ccgtgtcctc 360
tggcggtgga ggctctggcg gaggcggatc tggtggcgga ggatctgaca tcgagctgac 420
ccagtctccc gcctctctgt ctgcctctgt gggcgagacc gtgaccatca cctgtcgagc 480
ctctggcaac atccacaact acctggcctg gtatcagcag aagcagggca agtctcccca 540
gctgctggtg tactacacca ccaccctggc cgacggcgtg ccctctcgat tctctggctc 600
tggatctggc acccagtact ccctgaagat caactccctg cagcccgagg acttcggctc 660
ttactactgt cagcacttct ggtctacccc ccgaaccttc ggcggaggca ccaagctgga 720
gatcaagcga gcggccgc                                               738

<210> 8
<211> 738
<212> DNA
<213> Artificial Sequence

<220> 
<223> Yarrowia lipolytica codon optimized sequence

<400> 8
ggcccagccg gcccaggtgc agctgcagga atctggcccc ggactggtgg ccccctctca 60
gtctctgtct atcacctgta ccgtgtctgg cttctctctg accggctacg gcgtgaactg 120
ggtgcgacag ctgcctggca agggcctgga gtggctgggc atgatctggg gcgacggcaa 180
caccgcctac aactctgccc tgaagtctcg actgtctatc tctaaggaca actctaagtc 240
tcaggtgttc ctcaagatgg actctctcca caccgacgac accgcccgat actactgtgc 300
ccgagagcga gactaccgac tggactactg gggccagggc accaccgtga ccgtgtcctc 360
tggcggtgga ggctctggcg gaggcggatc tggtggcgga ggatctgaca tcaagctgac 420
ccagtctccc gcctctctgt ctgcctctgt gggcgagacc gtgaccatca cctgtcgagc 480
ctctggcaac acccacaact acctggcctg gtatcagcag aagcagggca agtctcccca 540
gctgctggtg tactacacca ccaccctggc cgacggcgtg ccctctcgat tctctggctc 600
tggatctggc acccagtact ccctgaagat caactccctg cagcccgagg acttcggctc 660
ttactactgt cagcacttct ggtctacccc ccgatctttc ggcggaggca ccaagctgga 720
gatcaagcga gcggccgc                                               738

<210> 9
<211> 675
<212> DNA
<213> Artificial Sequence

<220> 
<223> Yarrowia lipolytica codon optimized sequence

<400> 9
ggcccagccg gccgacgtga agctggacga gactggagga ggcctggtgc agcccggacg 60
acccatgaag ctgtcttgtg tggcctctgg cttcaccttc tctgactact ggatgaactg 120
ggtgcgacag tctcccgaga agggcctgga gtgggtggcc cagatccgaa acaagcccta 180
caactacgag acctactact ctgactctgt gaagggccga ttcaccatgt cccgagatga 240
ctctaagtcc tctgtgtacc tgcagatgaa caacctgcga gtggaggaca tgggcatcta 300
ctactgtacc ggctcttact acggcatgga ctactggggc cagggcacct ctgtgaccgt 360
gtcctctgct agcaccaagg gaccttctgt gtttcctctg gccccctctt ctaagtctac 420
ctctggtgga actgctgctc tgggatgtct ggtgaaggac tactttcctg agcctgtgac 480
tgtgtcttgg aactctggcg ctctgacttc tggtgttcac accttccctg ctgttctgca 540
gtcctctgga ctgtactctc tctcttctgt ggtgaccgtg ccttcttctt ctctgggaac 600
ccagacctac atctgtaacg tgaaccacaa gccctctaac actaaggtgg acaagcgagt 660
ggagcctgcg gccgc                                                  675

<210> 10
<211> 678
<212> DNA
<213> Artificial Sequence

<220> 
<223> Yarrowia lipolytica codon optimized sequence

<400> 10
ggcccagccg gccgacgtgg tgatgaccca gacccccctg tctctgcccg tgtctctggg 60
cgaccaggcc tctatctctt gtcgatcttc tcagtctctg gtccactcta acggcaacac 120
ctacctgcga tggtatctgc agaagcccgg ccagtctccc aaggtgctga tctacaaggt 180
gtctaaccga ttctctggcg tgcccgaccg attctccggc tctggctctg gcaccgactt 240
caccctgaag atctcccgag tggaggccga ggacctgggc gtgtacttct gttctcagtc 300
tacccacgtg ccctggacct tcggcggagg caccaagctg gagatcaagc gtacggtggc 360
tgctccttct gtgttcattt tccccccctc tgacgagcag ctgaagtctg gaactgcttc 420
tgttgtgtgc ctgctgaaca acttttaccc ccgagaggct aaggttcagt ggaaggtgga 480
caacgctctg cagtctggaa actctcagga gtctgttact gagcaggact ctaaggactc 540
gacctactct ctctcttcta ccctgaccct gtctaaggct gactacgaga agcataaggt 600
gtacgcttgt gaggttaccc atcagggact gtcctctccc gtgaccaagt cttttaaccg 660
aggcgagtgc gcggccgc                                               678

<210> 11
<211> 681
<212> DNA
<213> Artificial Sequence

<220> 
<223> Yarrowia lipolytica codon optimized sequence

<400> 11
ggcccagccg gccgaggtgc agctggtcga gtctggcggc ggactggtgc agcccggtgg 60
ctctctgcga ctgtcttgtg ccgcctctgg cttcaacatc aaggacacct acatccactg 120
ggtgcgacag gctcccggaa agggcctgga gtgggtggcc cgaatctacc ccaccaacgg 180
ctacacccga tacgccgact ctgtgaaggg ccgattcacc atctctgccg acacctctaa 240
gaacaccgcc tacctgcaga tgaactctct gcgagccgag gacaccgctg tgtactactg 300
ttctcgatgg ggaggcgacg gcttctacgc catggactac tggggccagg gcaccctggt 360
gaccgtgtcc tctgctagca ccaagggacc ttctgtgttt cctctggccc cctcttctaa 420
gtctacctct ggtggaactg ctgctctggg atgtctggtg aaggactact ttcctgagcc 480
tgtgactgtg tcttggaact ctggcgctct gacttctggt gttcacacct tccctgctgt 540
tctgcagtcc tctggactgt actctctctc ttctgtggtg accgtgcctt cttcttctct 600
gggaacccag acctacatct gtaacgtgaa ccacaagccc tctaacacta aggtggacaa 660
gcgagtggag cctgcggccg c                                           681

<210> 12
<211> 655
<212> DNA
<213> Artificial Sequence

<220> 
<223> Yarrowia lipolytica codon optimized sequence

<400> 12
ggcccagccg gccgacatcc agatgaccca gtctccctct tctctgtctg cctctgtggg 60
cgaccgagtg accatcacct gtcgagcctc tcaggacgtg aacaccgccg tggcctggta 120
tcagcagaag cccggcaagg cccccaagct gctgatctac tctgcctctt tcctgtactc 180
tggcgtgccc tctcgattct ctggctctcg atctggcacc gacttcaccc tgaccatctc 240
ttctctgcag cctgaggatt tcgccaccta ctactgtcag cagcactaca ccaccccccc 300
caccttcggc cagggaacca aggtggagat caagcgtacg gtggctgctc cttctgtgtt 360
cattttcccc ccctctgacg agcagctgaa gtctggaact gcttctgttg tgtgcctgct 420
gaacaacttt tacccccgag aggctaaggt tcagtggaag gtggacaacg ctctgcagtc 480
tggaaactct caggagtctg ttactgagca ggactctaag gactcgacct actctctctc 540
ttctaccctg accctgtcta aggctgacta cgagaagcat aaggtgtacg cttgtgaggt 600
tacccatcag ggactgtcct ctcccgtgac caagtctttt aaccgaggcg agtgc      655

<210> 13
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> Epitope tag

<400> 13
Gly Ala Pro Val Pro Tyr Pro Asp Pro Leu Glu Pro Arg
 1               5                  10              


<210> 14
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> Peptide linker

<400> 14
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
 1               5                  10                  15  


<210> 15
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> Peptide linker

<400> 15
Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser
 1               5                  10  