                         SEQUENCE LISTING

<110>  Expression Pathology Inc.
       Krizman, David
       Thyparambil, Sheeno
       Hembrough, Todd
 
<120>  Epidermal Growth Factor Receptor (EGFR) Protein SRM/MRM Assay

<130>  01152.8015

<150>  US 61/289,384
<151>  2009-12-22

<160>  74    

<170>  PatentIn version 3.5

<210>  1
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  1

Cys Asp Pro Ser Cys Pro Asn Gly Ser Cys Trp Gly Ala Gly Glu Glu 
1               5                   10                  15      


Asn Cys Gln Lys Leu Thr Lys Ile Ile Cys Ala Gln Gln Cys Ser Gly 
            20                  25                  30          


Arg 
    


<210>  2
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  2

Cys Glu Gly Pro Cys Arg Lys 
1               5           


<210>  3
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  3

Glu Asp Ser Phe Leu Gln Arg 
1               5           


<210>  4
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  4

Ile Pro Leu Glu Asn Leu Gln Ile Ile Arg 
1               5                   10  


<210>  5
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  5

Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys 
1               5                   10              


<210>  6
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  6

Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg 
1               5                   10                  15  


<210>  7
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  7

Glu Leu Arg Glu Ala Thr Ser Pro Lys Ala Asn Lys 
1               5                   10          


<210>  8
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  8

Glu Tyr His Ala Glu Gly Gly Lys 
1               5               


<210>  9
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  9

Phe Arg Glu Leu Ile Ile Glu Phe Ser Lys 
1               5                   10  


<210>  10
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  10

Gly Leu Trp Ile Pro Glu Gly Glu Lys 
1               5                   


<210>  11
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  11

Gly Leu Trp Ile Pro Glu Gly Glu Lys Val Lys Ile Pro Val Ala Ile 
1               5                   10                  15      


Lys 
    


<210>  12
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  12

His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro 
1               5                   10                  15      


Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro 
            20                  25                  30          


Gln Glu Leu Asp Ile Leu Lys 
        35                  


<210>  13
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  13

Ile Ile Cys Ala Gln Gln Cys Ser Gly Arg Cys Arg Gly Lys 
1               5                   10                  


<210>  14
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  14

Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu 
1               5                   10                  15      


Val Val Ala Leu Gly Ile Gly Leu Phe Met Arg Arg Arg 
            20                  25                  


<210>  15
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  15

Cys Glu Gly Pro Cys Arg 
1               5       


<210>  16
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  16

Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys 
1               5                   10  


<210>  17
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  17

Leu Thr Lys Ile Ile Cys Ala Gln Gln Cys Ser Gly Arg 
1               5                   10              


<210>  18
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  18

Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe 
1               5                   10                  15      


Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu 
            20                  25                  30          


Asp Ile Leu Lys 
        35      


<210>  19
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  19

Pro Tyr Asp Gly Ile Pro Ala Ser Glu Ile Ser Ser Ile Leu Glu Lys 
1               5                   10                  15      


Gly Glu Arg 
            


<210>  20
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  20

Pro Ala Gly Ser Val Gln Asn Pro Val Tyr His Asn Gln Pro Leu Asn 
1               5                   10                  15      


Pro Ala Pro Ser Arg 
            20      


<210>  21
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  21

Ser Pro Ser Asp Cys Cys His Asn Gln Cys Ala Ala Gly Cys Thr Gly 
1               5                   10                  15      


Pro Arg Glu Ser Asp Cys Leu Val Cys Arg 
            20                  25      


<210>  22
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  22

Ile Thr Asp Phe Gly Leu Ala Lys 
1               5               


<210>  23
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  23

Thr Pro Gln His Val Lys Ile Thr Asp Phe Gly Leu Ala Lys Leu Leu 
1               5                   10                  15      


Gly Ala Glu Glu Lys 
            20      


<210>  24
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  24

Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys 
1               5                   10      


<210>  25
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  25

Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile 
1               5                   10                  15      


Asn Ala Thr Asn Ile Lys His Phe Lys 
            20                  25  


<210>  26
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  26

Val Leu Gly Ser Gly Ala Phe Gly Thr Val Tyr Lys Gly Leu Trp Ile 
1               5                   10                  15      


Pro Glu Gly Glu Lys Val Lys 
            20              


<210>  27
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  27

Tyr Ser Phe Gly Ala Thr Cys Val Lys Lys Cys Pro Arg 
1               5                   10              


<210>  28
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  28

Cys Arg Gly Lys Ser Pro Ser Asp Cys Cys His Asn Gln Cys Ala Ala 
1               5                   10                  15      


Gly Cys Thr Gly Pro Arg 
            20          


<210>  29
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  29

Asp Ile Val Ser Ser Asp Phe Leu Ser Asn Met Ser Met Asp Phe Gln 
1               5                   10                  15      


Asn His Leu Gly Ser Cys Gln Lys 
            20                  


<210>  30
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  30

Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu 
1               5                   10                  15      


Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg 
            20                  25          


<210>  31
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  31

Glu Leu Ile Ile Glu Phe Ser Lys Met Ala Arg Asp Pro Gln Arg 
1               5                   10                  15  


<210>  32
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  32

Glu Leu Val Glu Pro Leu Thr Pro Ser Gly Glu Ala Pro Asn Gln Ala 
1               5                   10                  15      


Leu Leu Arg 
            


<210>  33
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  33

Glu Ser Asp Cys Leu Val Cys Arg Lys Phe Arg 
1               5                   10      


<210>  34
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  34

Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp 
1               5                   10                  15      


Ile Leu Lys 
            


<210>  35
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  35

Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys 
1               5                   10                  15      


Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg 
            20                  25          


<210>  36
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  36

Gly Lys Ser Pro Ser Asp Cys Cys His Asn Gln Cys Ala Ala Gly Cys 
1               5                   10                  15      


Thr Gly Pro Arg Glu Ser Asp Cys Leu Val Cys Arg 
            20                  25              


<210>  37
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  37

Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg 
1               5                   10                  15      


<210>  38
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  38

Ile Leu Lys Glu Thr Glu Phe Lys Lys 
1               5                   


<210>  39
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  39

Ile Pro Leu Glu Asn Leu Gln Ile Ile Arg 
1               5                   10  


<210>  40
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  40

Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys 
1               5                   10          


<210>  41
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  41

Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser 
1               5                   10                  15      


Ile Asn Ala Thr Asn Ile Lys 
            20              


<210>  42
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR peptide

<400>  42

Leu Leu Gln Glu Arg Glu Leu Val Glu Pro Leu Thr Pro Ser Gly Glu 
1               5                   10                  15      


Ala Pro Asn Gln Ala Leu Leu Arg 
            20                  


<210>  43
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR Peptide

<400>  43

Met His Leu Pro Ser Pro Thr Asp Ser Asn Phe Tyr Arg 
1               5                   10              


<210>  44
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR Peptide

<400>  44

Asn Val Leu Val Lys Thr Pro Gln His Val Lys Ile Thr Asp Phe Gly 
1               5                   10                  15      


Leu Ala Lys 
            


<210>  45
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR Peptide

<400>  45

Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys 
1               5                   10      


<210>  46
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR Peptide

<400>  46

Asn Tyr Asp Leu Ser Phe Leu Lys 
1               5               


<210>  47
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR Peptide

<400>  47

Pro Lys Phe Arg Glu Leu Ile Ile Glu Phe Ser Lys 
1               5                   10          


<210>  48
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR Peptide

<400>  48

Leu Leu Gln Glu Arg Glu Leu Val Glu Pro Leu Thr Pro Ser Gly Glu 
1               5                   10                  15      


Ala Pro Asn Gln Ala Leu Leu Arg 
            20                  


<210>  49
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR Peptide

<400>  49

Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys 
1               5                   10                  15      


<210>  50
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR Peptide

<400>  50

Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg 
1               5                   10              


<210>  51
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR Peptide

<400>  51

Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg 
1               5                   10                  15  


<210>  52
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR Peptide

<400>  52

Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile 
1               5                   10                  15      


Thr Ser Leu Gly Leu Arg 
            20          


<210>  53
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR Peptide

<400>  53

Thr Leu Arg Arg Leu Leu Gln Glu Arg 
1               5                   


<210>  54
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR Peptide

<400>  54

Thr Pro Leu Leu Ser Ser Leu Ser Ala Thr Ser Asn Asn Ser Thr Val 
1               5                   10                  15      


Ala Cys Ile Asp Arg 
            20      


<210>  55
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR Peptide

<400>  55

Val Ala Pro Gln Ser Ser Glu Phe Ile Gly Ala 
1               5                   10      


<210>  56
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR Peptide

<400>  56

Tyr Leu Val Ile Gln Gly Asp Glu Arg 
1               5                   


<210>  57
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR Peptide

<400>  57

Gly Ser Thr Ala Glu Asn Ala Glu Tyr Leu Arg 
1               5                   10      


<210>  58
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR Peptide


<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  PHOSPHORYLATION

<400>  58

Gly Ser Thr Ala Glu Asn Ala Glu Tyr Leu Arg 
1               5                   10      


<210>  59
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGRF Peptide

<400>  59

Gly Ser His Gln Ile Ser Leu Asp Asn Pro Asp Tyr Gln Gln Asp Asp 
1               5                   10                  15      


Phe Phe Pro Lys 
            20  


<210>  60
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGRF Peptide


<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  PHOSPHORYLATION

<400>  60

Gly Ser His Gln Ile Ser Leu Asp Asn Pro Asp Tyr Gln Gln Asp Asp 
1               5                   10                  15      


Phe Phe Pro Lys 
            20  


<210>  61
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGRF Peptide

<400>  61

Pro Ala Gly Ser Val Gln Asn Pro Val Tyr His Asn Gln Pro Leu Asn 
1               5                   10                  15      


Pro Ala Pro Ser Arg 
            20      


<210>  62
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGRF Peptide


<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  PHOSPHORYLATION

<400>  62

Pro Ala Gly Ser Val Gln Asn Pro Val Tyr His Asn Gln Pro Leu Asn 
1               5                   10                  15      


Pro Ala Pro Ser Arg 
            20      


<210>  63
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGRF Peptide

<400>  63

Glu Leu Val Glu Pro Leu Thr Pro Ser Gly Glu Ala Pro Asn Gln Ala 
1               5                   10                  15      


Leu Leu Arg 
            


<210>  64
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGRF Peptide


<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  PHOSPHORYLATION

<400>  64

Glu Leu Val Glu Pro Leu Thr Pro Ser Gly Glu Ala Pro Asn Gln Ala 
1               5                   10                  15      


Leu Leu Arg 
            


<210>  65
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGRF Peptide


<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  PHOSPHORYLATION

<400>  65

Glu Leu Val Glu Pro Leu Thr Pro Ser Gly Glu Ala Pro Asn Gln Ala 
1               5                   10                  15      


Leu Leu Arg 
            


<210>  66
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGRF Peptide


<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  PHOSPHORYLATION

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  PHOSPHORYLATION

<400>  66

Glu Leu Val Glu Pro Leu Thr Pro Ser Gly Glu Ala Pro Asn Gln Ala 
1               5                   10                  15      


Leu Leu Arg 
            


<210>  67
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGRF Peptide

<400>  67

Gly Ser His Gln Ile Ser Leu Asp Asn Pro Asp Tyr Gln Gln Asp Phe 
1               5                   10                  15      


Phe Pro Lys 
            


<210>  68
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGRF Peptide


<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  PHOSPHORYLATION

<400>  68

Gly Ser His Gln Ile Ser Leu Asp Asn Pro Asp Tyr Gln Gln Asp Phe 
1               5                   10                  15      


Phe Pro Lys 
            


<210>  69
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGRF Peptide

<400>  69

Tyr Ser Asp Pro Thr Gly Ala Leu Thr Glu Asp Ser Ile Asp Asp Thr 
1               5                   10                  15      


Phe Leu Pro Val Pro Glu Tyr Ile Asn Gln Ser Val Pro Lys 
            20                  25                  30  


<210>  70
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGRF Peptide


<220>
<221>  MOD_RES
<222>  (23)..(23)
<223>  PHOSPHORYLATION

<400>  70

Tyr Ser Asp Pro Thr Gly Ala Leu Thr Glu Asp Ser Ile Asp Asp Thr 
1               5                   10                  15      


Phe Leu Pro Val Pro Glu Tyr Ile Asn Gln Ser Val Pro Lys 
            20                  25                  30  


<210>  71
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGRF Peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  PHOSPHORYLATION

<400>  71

Tyr Ser Asp Pro Thr Gly Ala Leu Thr Glu Asp Ser Ile Asp Asp Thr 
1               5                   10                  15      


Phe Leu Pro Val Pro Glu Tyr Ile Asn Gln Ser Val Pro Lys 
            20                  25                  30  


<210>  72
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGRF Peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  PHOSPHORYLATION

<220>
<221>  MOD_RES
<222>  (23)..(23)
<223>  PHOSPHORYLATION

<400>  72

Tyr Ser Asp Pro Thr Gly Ala Leu Thr Glu Asp Ser Ile Asp Asp Thr 
1               5                   10                  15      


Phe Leu Pro Val Pro Glu Tyr Ile Asn Gln Ser Val Pro Lys 
            20                  25                  30  


<210>  73
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGRF Peptide

<400>  73

Glu Tyr His Ala Glu Gly Gly Lys 
1               5               


<210>  74
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGRF Peptide


<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  PHOSPHORYLATION

<400>  74

Glu Tyr His Ala Glu Gly Gly Lys 
1               5               


