                         SEQUENCE LISTING

<110>  Expression Pathology Inc.
       Krizman, David
       Thyparambil, Sheeno
       Hembrough, Todd
 
<120>  Insulin Receptor Substrate 1 (IRS1) Protein SRM/MRM Assay

<130>  01152.8013

<150>  US 61/289,382
<151>  2009-12-22

<160>  77    

<170>  PatentIn version 3.5

<210>  1
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  1

Glu Val Trp Gln Val Ile Leu Lys Pro Lys Gly Leu Gly Gln Thr Lys 
1               5                   10                  15      


<210>  2
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  2

Gly Leu Gly Gln Thr Lys Asn Leu Ile Gly Ile Tyr Arg Leu Cys Leu 
1               5                   10                  15      


Thr Ser Lys 
            


<210>  3
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  3

Gly Ser Gly Asp Tyr Met Pro Met Ser Pro Lys Ser Val Ser Ala Pro 
1               5                   10                  15      


Gln Gln Ile Ile Asn Pro Ile Arg 
            20                  


<210>  4
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  4

Leu Cys Gly Ala Ala Gly Gly Leu Glu Asn Gly Leu Asn Tyr Ile Asp 
1               5                   10                  15      


Leu Asp Leu Val Lys 
            20      


<210>  5
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  5

Leu Asn Ser Glu Ala Ala Ala Val Val Leu Gln Leu Met Asn Ile Arg 
1               5                   10                  15      


Arg 
    


<210>  6
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  6

Leu Trp Thr Asn Gly Val Gly Gly His His Ser His Val Leu Pro His 
1               5                   10                  15      


Pro Lys 
        


<210>  7
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  7

Asn Lys His Leu Val Ala Leu Tyr Thr Arg 
1               5                   10  


<210>  8
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  8

Pro Lys Gly Leu Gly Gln Thr Lys Asn Leu Ile Gly Ile Tyr Arg 
1               5                   10                  15  


<210>  9
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  9

Arg Ser Ile Pro Leu Glu Ser Cys Phe Asn Ile Asn Lys 
1               5                   10              


<210>  10
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  10

Arg Thr His Ser Ala Gly Thr Ser Pro Thr Ile Thr His Gln Lys 
1               5                   10                  15  


<210>  11
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  11

Ser Gln Ser Ser Ser Asn Cys Ser Asn Pro Ile Ser Val Pro Leu Arg 
1               5                   10                  15      


Arg His His Leu Asn Asn Pro Pro Pro Ser Gln Val Gly Leu Thr Arg 
            20                  25                  30          


<210>  12
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  12

Ser Val Ser Ala Pro Gln Gln Ile Ile Asn Pro Ile Arg Arg 
1               5                   10                  


<210>  13
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  13

Thr Ile Ser Phe Val Lys Leu Asn Ser Glu Ala Ala Ala Val Val Leu 
1               5                   10                  15      


Gln Leu Met Asn Ile Arg 
            20          


<210>  14
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  14

Val Asp Thr Ala Ala Gln Thr Asn Ser Arg Leu Ala Arg 
1               5                   10              


<210>  15
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  15

Val Ile Arg Ala Asp Pro Gln Gly Cys Arg Arg 
1               5                   10      


<210>  16
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  16

Ala Ala Ser Glu Ala Gly Gly Pro Ala Arg Leu Glu Tyr Tyr Glu Asn 
1               5                   10                  15      


Glu Lys 
        


<210>  17
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  17

Ala Ala Trp Gln Glu Ser Thr Gly Val Glu Met Gly Arg 
1               5                   10              


<210>  18
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  18

Ala Ala Trp Gln Glu Ser Thr Gly Val Glu Met Gly Arg Leu Gly Pro 
1               5                   10                  15      


Ala Pro Pro Gly Ala Ala Ser Ile Cys Arg 
            20                  25      


<210>  19
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  19

Ala Asp Pro Gln Gly Cys Arg 
1               5           


<210>  20
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  20

Ala Met Ser Asp Glu Phe Arg Pro Arg Ser Lys 
1               5                   10      


<210>  21
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  21

Ala Arg Glu Gln Gln Gln Gln Gln Gln Pro Leu Leu His Pro Pro Glu 
1               5                   10                  15      


Pro Lys 
        


<210>  22
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  22

Ala Ser Ser Asp Gly Glu Gly Thr Met Ser Arg Pro Ala Ser Val Asp 
1               5                   10                  15      


Gly Ser Pro Val Ser Pro Ser Thr Asn Arg 
            20                  25      


<210>  23
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  23

Cys Pro Ser Gln Leu Gln Pro Ala Pro Arg 
1               5                   10  


<210>  24
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  24

Glu Glu Glu Thr Gly Thr Glu Glu Tyr Met Lys 
1               5                   10      


<210>  25
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  25

Cys Thr Pro Gly Thr Gly Leu Gly Thr Ser Pro Ala Leu Ala Gly Asp 
1               5                   10                  15      


Glu Ala Ala Ser Ala Ala Asp Leu Asp Asn Arg 
            20                  25          


<210>  26
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  26

Met Asp Leu Gly Pro Gly Arg Arg 
1               5               


<210>  27
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  27

Phe Phe Val Leu Arg Ala Ala Ser Glu Ala Gly Gly Pro Ala Arg 
1               5                   10                  15  


<210>  28
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  28

Gly Gly Asn Gly His Arg Cys Thr Pro Gly Thr Gly Leu Gly Thr Ser 
1               5                   10                  15      


Pro Ala Leu Ala Gly Asp Glu Ala Ala Ser Ala Ala Asp Leu Asp Asn 
            20                  25                  30          


Arg 
    


<210>  29
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  29

His His Leu Asn Asn Pro Pro Pro Ser Gln Val Gly Leu Thr Arg 
1               5                   10                  15  


<210>  30
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  30

His Ser Ala Phe Val Pro Thr Arg Ser Tyr Pro Glu Glu Gly Leu Glu 
1               5                   10                  15      


Met His Pro Leu Glu Arg 
            20          


<210>  31
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  31

Gly Ser Gly Asp Tyr Met Pro Met Ser Pro Lys 
1               5                   10      


<210>  32
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  32

Val Asp Thr Ala Ala Gln Thr Asn Ser Arg 
1               5                   10  


<210>  33
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  33

Lys Val Gly Tyr Leu Arg Lys 
1               5           


<210>  34
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  34

Leu Ala Arg Pro Thr Arg Leu Ser Leu Gly Asp Pro Lys 
1               5                   10              


<210>  35
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  35

Leu His Pro Pro Leu Asn His Ser Arg Ser Ile Pro Met Pro Ala Ser 
1               5                   10                  15      


Arg Cys Ser Pro Ser Ala Thr Ser Pro Val Ser Leu Ser Ser Ser Ser 
            20                  25                  30          


Thr Ser Gly His Gly Ser Thr Ser Asp Cys Leu Phe Pro Arg 
        35                  40                  45      


<210>  36
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  36

Leu Leu Tyr Ala Ala Thr Ala Asp Asp Ser Ser Ser Ser Thr Ser Ser 
1               5                   10                  15      


Asp Ser Leu Gly Gly Gly Tyr Cys Gly Ala Arg 
            20                  25          


<210>  37
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  37

Leu Ser Leu Gly Asp Pro Lys Ala Ser Thr Leu Pro Arg 
1               5                   10              


<210>  38
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  38

Leu Ser Thr Ser Ser Gly Arg 
1               5           


<210>  39
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  39

Pro Ala Ser Val Asp Gly Ser Pro Val Ser Pro Ser Thr Asn Arg Thr 
1               5                   10                  15      


His Ala His Arg 
            20  


<210>  40
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  40

Pro Asp Ser Ser Thr Leu His Thr Asp Asp Gly Tyr Met Pro Met Ser 
1               5                   10                  15      


Pro Gly Val Ala Pro Val Pro Ser Gly Arg 
            20                  25      


<210>  41
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  41

Pro Gly Glu Leu Gly Gly Ala Pro Lys 
1               5                   


<210>  42
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  42

Pro Arg Ser Lys Ser Gln Ser Ser Ser Asn Cys Ser Asn Pro Ile Ser 
1               5                   10                  15      


Val Pro Leu Arg 
            20  


<210>  43
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  43

Pro Thr Arg Leu Ser Leu Gly Asp Pro Lys Ala Ser Thr Leu Pro Arg 
1               5                   10                  15      


<210>  44
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  44

Gln Ser Tyr Val Asp Thr Ser Pro Ala Ala Pro Val Ser Tyr Ala Asp 
1               5                   10                  15      


Met Arg 
        


<210>  45
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  45

Arg His His Leu Asn Asn Pro Pro Pro Ser Gln Val Gly Leu Thr Arg 
1               5                   10                  15      


<210>  46
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  46

His Ser Ser Glu Thr Phe Ser Ser Thr Pro Ser Ala Thr Arg 
1               5                   10                  


<210>  47
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  47

Arg Ser Arg Thr Glu Ser Ile Thr Ala Thr Ser Pro Ala Ser Met Val 
1               5                   10                  15      


Gly Gly Lys 
            


<210>  48
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  48

Arg Ser Ser Glu Asp Leu Ser Ala Tyr Ala Ser Ile Ser Phe Gln Lys 
1               5                   10                  15      


<210>  49
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  49

Ser Ile Pro Leu Glu Ser Cys Phe Asn Ile Asn Lys 
1               5                   10          


<210>  50
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  50

Ser Lys Ser Gln Ser Ser Ser Asn Cys Ser Asn Pro Ile Ser Val Pro 
1               5                   10                  15      


Leu Arg 
        


<210>  51
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  51

Ser Arg Thr Glu Ser Ile Thr Ala Thr Ser Pro Ala Ser Met Val Gly 
1               5                   10                  15      


Gly Lys 
        


<210>  52
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  52

Ser Ser Ala Ser Val Ser Gly Ser Pro Ser Asp Gly Gly Phe Ile Ser 
1               5                   10                  15      


Ser Asp Glu Tyr Gly Ser Ser Pro Cys Asp Phe Arg 
            20                  25              


<210>  53
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  53

Ser Ser Glu Asp Leu Ser Ala Tyr Ala Ser Ile Ser Phe Gln Lys Gln 
1               5                   10                  15      


Pro Glu Asp Arg 
            20  


<210>  54
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  54

Ser Ser Phe Arg Ser Val Thr Pro Asp Ser Leu Gly His Thr Pro Pro 
1               5                   10                  15      


Ala 
    


<210>  55
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  55

Gly Glu Glu Glu Leu Ser Asn Tyr Ile Cys Met Gly Gly Lys 
1               5                   10                  


<210>  56
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  56

Ser Val Thr Pro Asp Ser Leu Gly His Thr Pro Pro Ala Arg 
1               5                   10                  


<210>  57
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  57

Ser Tyr Pro Glu Glu Gly Leu Glu Met His Pro Leu Glu Arg 
1               5                   10                  


<210>  58
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  58

Thr Glu Ser Ile Thr Ala Thr Ser Pro Ala Ser Met Val Gly Gly Lys 
1               5                   10                  15      


<210>  59
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  59

Val Gly Asn Thr Val Pro Phe Gly Ala Gly Ala Ala Val Gly Gly Gly 
1               5                   10                  15      


Gly Gly Ser Ser Ser Ser Ser Glu Asp Val Lys 
            20                  25          


<210>  60
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  60

Val Asn Leu Ser Pro Asn Arg Asn Gln Ser Ala Lys 
1               5                   10          


<210>  61
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  61

Gly Ser Gly Asp Tyr Met Pro Met Ser Pro Lys 
1               5                   10      


<210>  62
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  62

Ala Ser Ser Asp Gly Glu Gly Thr Met Ser Arg Pro Ala Ser Val Asp 
1               5                   10                  15      


Gly Ser Pro Val Ser Pro Ser Thr Asn Arg 
            20                  25      


<210>  63
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  63

Ser Val Ser Ala Pro Gln Gln Ile Ile Asn Pro Ile Arg 
1               5                   10              


<210>  64
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  64

Leu Cys Leu Thr Ser Lys Thr Ile Ser Phe Val Lys Leu Asn Ser Glu 
1               5                   10                  15      


Ala Ala Ala Val Val Leu Gln Leu Met Asn Ile Arg 
            20                  25              


<210>  65
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  65

Leu Glu Pro Ser Leu Pro His Pro His His Gln Val Leu Gln Pro His 
1               5                   10                  15      


Leu Pro Arg 
            


<210>  66
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  66

Leu Pro Gly His Arg His Ser Ala Phe Val Pro Thr Arg 
1               5                   10              


<210>  67
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  67

Ser Ser Glu Asp Leu Ser Ala Tyr Ala Ser Ile Ser Phe Gln Lys 
1               5                   10                  15  


<210>  68
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide


<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  PHOSPHORYLATION

<400>  68

Pro Asp Ser Ser Thr Leu His Thr Asp Asp Gly Tyr Met Pro Met Ser 
1               5                   10                  15      


Pro Gly Val Ala Pro Val Pro Ser Gly Arg 
            20                  25      


<210>  69
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide


<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  PHOSPHORYLATION

<400>  69

Ser Pro Gly Glu Tyr Val Asn Ile Glu Phe Gly Ser Asp Gln Ser Gly 
1               5                   10                  15      


Tyr Leu Ser Gly Pro Val Ala Phe His Ser Ser Pro Ser Val Arg 
            20                  25                  30      


<210>  70
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  70

Glu Gln Gln Gln Gln Gln Gln Pro Leu Leu His Pro Pro Glu Pro Lys 
1               5                   10                  15      


<210>  71
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  71

His Ser Ser Ala Ser Phe Glu Asn Val Trp Leu Arg Pro Gly Glu Leu 
1               5                   10                  15      


Gly Gly Ala Pro Lys 
            20      


<210>  72
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  72

Leu Glu Tyr Tyr Glu Asn Glu Lys 
1               5               


<210>  73
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  73

Leu Asn Ser Glu Ala Ala Ala Val Val Leu Gln Leu Met Asn Ile Arg 
1               5                   10                  15      


<210>  74
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  74

Leu Ser Leu Gly Asp Pro Lys 
1               5           


<210>  75
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  75

Asn Leu Ile Gly Ile Tyr Arg 
1               5           


<210>  76
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  76

Thr Gly Ile Ala Ala Glu Glu Val Ser Leu Pro Arg 
1               5                   10          


<210>  77
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IRS Peptide

<400>  77

His Leu Val Ala Leu Tyr Thr Arg 
1               5               


