                         SEQUENCE LISTING

<110>  Novozymes A/S
       Novozymes North America, Inc.
 
<120>  Pullulanase variant and Uses thereof

<130>  11653-WO-PCT

<150>  US 61/289,040
<151>  2009-12-22

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  4014
<212>  DNA
<213>  Thermococcus hydrothermalis


<220>
<221>  CDS
<222>  (1)..(4011)

<220>
<221>  sig_peptide
<222>  (1)..(81)

<220>
<221>  mat_peptide
<222>  (82)..(4011)

<400>  1
atg agg cgg gtg gtt gcc ctc ttc att gca att ttg atg ctt gga agc         48
Met Arg Arg Val Val Ala Leu Phe Ile Ala Ile Leu Met Leu Gly Ser           
        -25                 -20                 -15                       

atc gtt gga gcg aac gtt aag agc gtt ggc gcg gcg gag ccg aag ccg         96
Ile Val Gly Ala Asn Val Lys Ser Val Gly Ala Ala Glu Pro Lys Pro           
    -10                 -5              -1  1               5             

ctc aac gtc ata ata gtc tgg cac cag cac cag ccc tac tac tac gac        144
Leu Asn Val Ile Ile Val Trp His Gln His Gln Pro Tyr Tyr Tyr Asp           
                10                  15                  20                

cct gtc cag gac gtc tac acc agg ccc tgg gtc agg ctc cac gcg gcg        192
Pro Val Gln Asp Val Tyr Thr Arg Pro Trp Val Arg Leu His Ala Ala           
            25                  30                  35                    

aac aac tac tgg aag atg gcc cac tac ctg agc cag tac ccg gag gtt        240
Asn Asn Tyr Trp Lys Met Ala His Tyr Leu Ser Gln Tyr Pro Glu Val           
        40                  45                  50                        

cac gcc acc att gac ctc tcg ggt tcg ctg ata gcc cag ctt gcc gac        288
His Ala Thr Ile Asp Leu Ser Gly Ser Leu Ile Ala Gln Leu Ala Asp           
    55                  60                  65                            

tac atg aac ggc aag aag gac acc tac cag ata atc acc gag aag ata        336
Tyr Met Asn Gly Lys Lys Asp Thr Tyr Gln Ile Ile Thr Glu Lys Ile           
70                  75                  80                  85            

gcc aac ggg gaa ccc ctc acc gtc gac gag aag tgg ttc atg ctc cag        384
Ala Asn Gly Glu Pro Leu Thr Val Asp Glu Lys Trp Phe Met Leu Gln           
                90                  95                  100               

gca ccg gga ggg ttc ttc gac aac acc atc ccc tgg aac ggt gaa ccg        432
Ala Pro Gly Gly Phe Phe Asp Asn Thr Ile Pro Trp Asn Gly Glu Pro           
            105                 110                 115                   

ata acc gac ccc aac ggc aac ccg ata agg gac ttc tgg gac cgc tac        480
Ile Thr Asp Pro Asn Gly Asn Pro Ile Arg Asp Phe Trp Asp Arg Tyr           
        120                 125                 130                       

acg gag ctg aag aac aag atg ctc agc gca aag gcc aag tac gca aac        528
Thr Glu Leu Lys Asn Lys Met Leu Ser Ala Lys Ala Lys Tyr Ala Asn           
    135                 140                 145                           

ttc gtg act gag agc cag aag gtc gct gtg acg aac gag ttc aca gag        576
Phe Val Thr Glu Ser Gln Lys Val Ala Val Thr Asn Glu Phe Thr Glu           
150                 155                 160                 165           

cag gac tac ata gac cta gcg gtt ctc ttc aat ctc gct tgg att gac        624
Gln Asp Tyr Ile Asp Leu Ala Val Leu Phe Asn Leu Ala Trp Ile Asp           
                170                 175                 180               

tac aat tac atc acg agc acg ccg gag ttc aag gcc ctc tac gac aag        672
Tyr Asn Tyr Ile Thr Ser Thr Pro Glu Phe Lys Ala Leu Tyr Asp Lys           
            185                 190                 195                   

gtt gac gag ggc ggc tat aca agg gcg gac gtc aaa acc gtt ctc gac        720
Val Asp Glu Gly Gly Tyr Thr Arg Ala Asp Val Lys Thr Val Leu Asp           
        200                 205                 210                       

gcc cag atc tgg ctt ctc aac cac acc ttc gag gag cac gag aag ata        768
Ala Gln Ile Trp Leu Leu Asn His Thr Phe Glu Glu His Glu Lys Ile           
    215                 220                 225                           

aac ctc ctc ctc gga aac ggc aac gtc gag gtc acg gtc gtt ccc tac        816
Asn Leu Leu Leu Gly Asn Gly Asn Val Glu Val Thr Val Val Pro Tyr           
230                 235                 240                 245           

gcc cac ccg ata ggc ccg ata ctc aac gac ttc ggc tgg gac agc gac        864
Ala His Pro Ile Gly Pro Ile Leu Asn Asp Phe Gly Trp Asp Ser Asp           
                250                 255                 260               

ttc aac gac cag gtc aag aag gcc gac gaa ctg tac aag ccg tac ctc        912
Phe Asn Asp Gln Val Lys Lys Ala Asp Glu Leu Tyr Lys Pro Tyr Leu           
            265                 270                 275                   

ggc ggc ggc acc gcg gtt cca aaa ggc gga tgg gcg gct gag agc gcc        960
Gly Gly Gly Thr Ala Val Pro Lys Gly Gly Trp Ala Ala Glu Ser Ala           
        280                 285                 290                       

ctc aac gac aaa act ctg gag atc ctc gcc gag aac ggc tgg gag tgg       1008
Leu Asn Asp Lys Thr Leu Glu Ile Leu Ala Glu Asn Gly Trp Glu Trp           
    295                 300                 305                           

gtc atg acc gac cag atg gtt ctc gga aag ctc ggc att gag gga acc       1056
Val Met Thr Asp Gln Met Val Leu Gly Lys Leu Gly Ile Glu Gly Thr           
310                 315                 320                 325           

gtc gag aac tac cac aag ccc tgg gtg gcc gag ttc aac gga aag aag       1104
Val Glu Asn Tyr His Lys Pro Trp Val Ala Glu Phe Asn Gly Lys Lys           
                330                 335                 340               

ata tac ctc ttc cca aga aat cac gat cta agt gac aga gtt ggc ttt       1152
Ile Tyr Leu Phe Pro Arg Asn His Asp Leu Ser Asp Arg Val Gly Phe           
            345                 350                 355                   

acc tac agc gga atg aac cag cag cag gcc gtt gag gac ttc gtc aac       1200
Thr Tyr Ser Gly Met Asn Gln Gln Gln Ala Val Glu Asp Phe Val Asn           
        360                 365                 370                       

gag ctc ctc aag ctc cag aag cag aac tac gat ggc tcg ctg gtt tac       1248
Glu Leu Leu Lys Leu Gln Lys Gln Asn Tyr Asp Gly Ser Leu Val Tyr           
    375                 380                 385                           

gtg gtc acg ctc gac ggc gag aac ccc gtg gag aac tac ccc tac gac       1296
Val Val Thr Leu Asp Gly Glu Asn Pro Val Glu Asn Tyr Pro Tyr Asp           
390                 395                 400                 405           

ggg gag ctc ttc ctc acc gaa ctc tac aag aag ctg acc gaa ctc cag       1344
Gly Glu Leu Phe Leu Thr Glu Leu Tyr Lys Lys Leu Thr Glu Leu Gln           
                410                 415                 420               

gag cag ggt ctc ata aga acc ctc acc ccg agc gag tac atc cag ctc       1392
Glu Gln Gly Leu Ile Arg Thr Leu Thr Pro Ser Glu Tyr Ile Gln Leu           
            425                 430                 435                   

tac ggc gac aag gcc aac aag ctc aca cct cgg atg atg gag cgc ctt       1440
Tyr Gly Asp Lys Ala Asn Lys Leu Thr Pro Arg Met Met Glu Arg Leu           
        440                 445                 450                       

gac ctc acc gga gac aac gtt aac gcc ctc ctc aag gcc cag agc ctc       1488
Asp Leu Thr Gly Asp Asn Val Asn Ala Leu Leu Lys Ala Gln Ser Leu           
    455                 460                 465                           

ggc gaa ctc tac gac atg acc ggc gtt aag gag gag atg cag tgg ccc       1536
Gly Glu Leu Tyr Asp Met Thr Gly Val Lys Glu Glu Met Gln Trp Pro           
470                 475                 480                 485           

gag agc agc tgg ata gac gga acc ctc tcc acg tgg ata ggc gag ccc       1584
Glu Ser Ser Trp Ile Asp Gly Thr Leu Ser Thr Trp Ile Gly Glu Pro           
                490                 495                 500               

cag gag aac tac ggc tgg tac tgg ctc tac atg gcc agg aag gcc ctt       1632
Gln Glu Asn Tyr Gly Trp Tyr Trp Leu Tyr Met Ala Arg Lys Ala Leu           
            505                 510                 515                   

atg gag aac aag gat aaa atg agc cag gcg gac tgg gag aag gcc tac       1680
Met Glu Asn Lys Asp Lys Met Ser Gln Ala Asp Trp Glu Lys Ala Tyr           
        520                 525                 530                       

gag tac ctg ctc cgc gcc gag gca agc gac tgg ttc tgg tgg tac gga       1728
Glu Tyr Leu Leu Arg Ala Glu Ala Ser Asp Trp Phe Trp Trp Tyr Gly           
    535                 540                 545                           

agc gac cag gac agc ggc cag gac tac acc ttc gac cgc tac ctg aag       1776
Ser Asp Gln Asp Ser Gly Gln Asp Tyr Thr Phe Asp Arg Tyr Leu Lys           
550                 555                 560                 565           

acc tac ctc tac gag atg tac aag ctg gca gga gtc gag ccg ccg agc       1824
Thr Tyr Leu Tyr Glu Met Tyr Lys Leu Ala Gly Val Glu Pro Pro Ser           
                570                 575                 580               

tac ctc ttc ggc aac tac ttc ccg gac gga gag ccc tac acc acg agg       1872
Tyr Leu Phe Gly Asn Tyr Phe Pro Asp Gly Glu Pro Tyr Thr Thr Arg           
            585                 590                 595                   

ggc ctg gtc gga ctc aag gac ggc gag atg aag aac ttc tcc agc atg       1920
Gly Leu Val Gly Leu Lys Asp Gly Glu Met Lys Asn Phe Ser Ser Met           
        600                 605                 610                       

tcc ccg ctg gca aag ggc gtg agc gtc tat ttc gac ggc gag ggg ata       1968
Ser Pro Leu Ala Lys Gly Val Ser Val Tyr Phe Asp Gly Glu Gly Ile           
    615                 620                 625                           

cac ttc ata gtg aaa ggg aac ctg gac agg ttc gag gtg agc atc tgg       2016
His Phe Ile Val Lys Gly Asn Leu Asp Arg Phe Glu Val Ser Ile Trp           
630                 635                 640                 645           

gag aag gat gag cgc gtt ggc aac acg ttc acc cgc ctc caa gag aag       2064
Glu Lys Asp Glu Arg Val Gly Asn Thr Phe Thr Arg Leu Gln Glu Lys           
                650                 655                 660               

ccg gac gag ttg agc tat ttc atg ttc cca ttc tca agg gac agc gtt       2112
Pro Asp Glu Leu Ser Tyr Phe Met Phe Pro Phe Ser Arg Asp Ser Val           
            665                 670                 675                   

ggt ctc ctc ata acc aag cac gtc gtg tac gag aac gga aag gcc gag       2160
Gly Leu Leu Ile Thr Lys His Val Val Tyr Glu Asn Gly Lys Ala Glu           
        680                 685                 690                       

ata tac ggc gcc acc gac tac gag aag agc gag aag ctt ggg gaa gcc       2208
Ile Tyr Gly Ala Thr Asp Tyr Glu Lys Ser Glu Lys Leu Gly Glu Ala           
    695                 700                 705                           

acc gtc aag aac acg agc gaa gga atc gaa gtc gtc ctt ccc ttt gac       2256
Thr Val Lys Asn Thr Ser Glu Gly Ile Glu Val Val Leu Pro Phe Asp           
710                 715                 720                 725           

tac ata gaa aac ccc tcc gac ttc tac ttc gct gtc tcg acg gtc aaa       2304
Tyr Ile Glu Asn Pro Ser Asp Phe Tyr Phe Ala Val Ser Thr Val Lys           
                730                 735                 740               

gat gga gac ctt gag gtg ata agc act cct gtg gag ctc aag ctc ccg       2352
Asp Gly Asp Leu Glu Val Ile Ser Thr Pro Val Glu Leu Lys Leu Pro           
            745                 750                 755                   

acc gag gtc aag gga gtc gtc ata gcc gat ata acc gac cca gaa ggc       2400
Thr Glu Val Lys Gly Val Val Ile Ala Asp Ile Thr Asp Pro Glu Gly           
        760                 765                 770                       

gac gac cat ggg ccc gga aac tac act tat ccc acg gac aag gtc ttc       2448
Asp Asp His Gly Pro Gly Asn Tyr Thr Tyr Pro Thr Asp Lys Val Phe           
    775                 780                 785                           

aag cca ggt gtt ttc gac ctc ctc cgc ttc agg atg ctc gaa cag acg       2496
Lys Pro Gly Val Phe Asp Leu Leu Arg Phe Arg Met Leu Glu Gln Thr           
790                 795                 800                 805           

gag agc tac gtc atg gag ttc tac ttc aag gac cta ggt ggt aac ccg       2544
Glu Ser Tyr Val Met Glu Phe Tyr Phe Lys Asp Leu Gly Gly Asn Pro           
                810                 815                 820               

tgg aac gga ccc aac ggc ttc agc ctc cag ata atc gag gtc tac ctc       2592
Trp Asn Gly Pro Asn Gly Phe Ser Leu Gln Ile Ile Glu Val Tyr Leu           
            825                 830                 835                   

gac ttc aag gac ggt gga aac agt tcg gcc att aag atg ttc ccc gac       2640
Asp Phe Lys Asp Gly Gly Asn Ser Ser Ala Ile Lys Met Phe Pro Asp           
        840                 845                 850                       

gga ccg gga gcc aac gtc aac ctc gac ccc gag cat cca tgg gac gtt       2688
Gly Pro Gly Ala Asn Val Asn Leu Asp Pro Glu His Pro Trp Asp Val           
    855                 860                 865                           

gcc ttc agg ata gcg ggc tgg gac tac gga aac ctc atc atc ctg ccg       2736
Ala Phe Arg Ile Ala Gly Trp Asp Tyr Gly Asn Leu Ile Ile Leu Pro           
870                 875                 880                 885           

aac gga acg gcc atc cag ggc gag atg cag att tcc gca gat ccg gtt       2784
Asn Gly Thr Ala Ile Gln Gly Glu Met Gln Ile Ser Ala Asp Pro Val           
                890                 895                 900               

aag aac gcc ata ata gtc aag gtt cca aag aag tac atc gcc ata aac       2832
Lys Asn Ala Ile Ile Val Lys Val Pro Lys Lys Tyr Ile Ala Ile Asn           
            905                 910                 915                   

gag gac tac ggc ctc tgg gga gac gtc ctc gtc ggc tcg cag gac ggc       2880
Glu Asp Tyr Gly Leu Trp Gly Asp Val Leu Val Gly Ser Gln Asp Gly           
        920                 925                 930                       

tac ggc ccg gac aag tgg aga acg gcg gca gtg gat gcg gag cag tgg       2928
Tyr Gly Pro Asp Lys Trp Arg Thr Ala Ala Val Asp Ala Glu Gln Trp           
    935                 940                 945                           

aag ctt gga ggt gcg gac ccg cag gca gtc ata aac ggc gtg gcc ccg       2976
Lys Leu Gly Gly Ala Asp Pro Gln Ala Val Ile Asn Gly Val Ala Pro           
950                 955                 960                 965           

cgc gtc att gat gag ctg gtt ccg cag ggc ttt gaa ccg acc cag gag       3024
Arg Val Ile Asp Glu Leu Val Pro Gln Gly Phe Glu Pro Thr Gln Glu           
                970                 975                 980               

gag cag ctg agc agc tac gat gca aac gac atg aag ctc gcc act gtc       3072
Glu Gln Leu Ser Ser Tyr Asp Ala Asn Asp Met Lys Leu Ala Thr Val           
            985                 990                 995                   

aag gcg ctg  cta ctc ctc aag cag  ggc atc gtt gtg acc  gac ccg        3117
Lys Ala Leu  Leu Leu Leu Lys Gln  Gly Ile Val Val Thr  Asp Pro            
        1000                 1005                 1010                    

gag gga gac  gac cac ggg ccg gga  acg tac acc tat ccg  acg gac        3162
Glu Gly Asp  Asp His Gly Pro Gly  Thr Tyr Thr Tyr Pro  Thr Asp            
        1015                 1020                 1025                    

aaa gtt ttc  aag ccc ggt gtt ttc  gac ctc ctc aag ttc  aag gtg        3207
Lys Val Phe  Lys Pro Gly Val Phe  Asp Leu Leu Lys Phe  Lys Val            
        1030                 1035                 1040                    

acc gag gga  agc gac gac tgg acg  ctg gag ttc cac ttc  aaa gac        3252
Thr Glu Gly  Ser Asp Asp Trp Thr  Leu Glu Phe His Phe  Lys Asp            
        1045                 1050                 1055                    

ctc ggt gga  aac ccg tgg aac ggg  ccg aac ggc ttc agc  ctg cag        3297
Leu Gly Gly  Asn Pro Trp Asn Gly  Pro Asn Gly Phe Ser  Leu Gln            
        1060                 1065                 1070                    

ata atc gag  gta tac ttc gac ttc  aag gag ggc ggg aac  gtc tcg        3342
Ile Ile Glu  Val Tyr Phe Asp Phe  Lys Glu Gly Gly Asn  Val Ser            
        1075                 1080                 1085                    

gcc att aag  atg ttc ccg gat ggg  ccc gga agc aac gtc  cgt ctt        3387
Ala Ile Lys  Met Phe Pro Asp Gly  Pro Gly Ser Asn Val  Arg Leu            
        1090                 1095                 1100                    

gat cca aat  cac cca tgg gac ctg  gcg ctt agg ata gcc  ggc tgg        3432
Asp Pro Asn  His Pro Trp Asp Leu  Ala Leu Arg Ile Ala  Gly Trp            
        1105                 1110                 1115                    

gac tac gga  aac ctg ata att ctg  ccc gac gga acc gcc  tac caa        3477
Asp Tyr Gly  Asn Leu Ile Ile Leu  Pro Asp Gly Thr Ala  Tyr Gln            
        1120                 1125                 1130                    

ggc gag atg  cag att tcc gca gat  ccg gtt aag aac gcc  ata ata        3522
Gly Glu Met  Gln Ile Ser Ala Asp  Pro Val Lys Asn Ala  Ile Ile            
        1135                 1140                 1145                    

gtc aag gtt  cca aag aag tac ctg  aac ata tcc gac tac  gga ctc        3567
Val Lys Val  Pro Lys Lys Tyr Leu  Asn Ile Ser Asp Tyr  Gly Leu            
        1150                 1155                 1160                    

tac acc gcc  gtc atc gtg ggt tcc  caa gac ggg tac ggc  ccg gac        3612
Tyr Thr Ala  Val Ile Val Gly Ser  Gln Asp Gly Tyr Gly  Pro Asp            
        1165                 1170                 1175                    

aag tgg agg  ccc gtg gcc gct gag  gcc gag cag tgg aag  ctc gga        3657
Lys Trp Arg  Pro Val Ala Ala Glu  Ala Glu Gln Trp Lys  Leu Gly            
        1180                 1185                 1190                    

ggc gca gac  ccc cag gcg gtc ata  gac aac ctc gta cca  agg gtc        3702
Gly Ala Asp  Pro Gln Ala Val Ile  Asp Asn Leu Val Pro  Arg Val            
        1195                 1200                 1205                    

gtt gat gaa  ctc gtg ccg gag ggc  ttc aag cca acg cag  gag gag        3747
Val Asp Glu  Leu Val Pro Glu Gly  Phe Lys Pro Thr Gln  Glu Glu            
        1210                 1215                 1220                    

cag ctg agc  agc tac gac ctt gag  aag aag acc ctg gcg  acg gtg        3792
Gln Leu Ser  Ser Tyr Asp Leu Glu  Lys Lys Thr Leu Ala  Thr Val            
        1225                 1230                 1235                    

ctc atg gta  ccg ctc gtc aat ggg  act ggc ggc gag gaa  cca acg        3837
Leu Met Val  Pro Leu Val Asn Gly  Thr Gly Gly Glu Glu  Pro Thr            
        1240                 1245                 1250                    

ccg acg gag  agc cca acg gaa acg  acg aca acc aca ccc  agc gaa        3882
Pro Thr Glu  Ser Pro Thr Glu Thr  Thr Thr Thr Thr Pro  Ser Glu            
        1255                 1260                 1265                    

aca acc acc  aca act tca acg acc  acc ggc cca agc tca  acg acc        3927
Thr Thr Thr  Thr Thr Ser Thr Thr  Thr Gly Pro Ser Ser  Thr Thr            
        1270                 1275                 1280                    

acc agc aca  ccc ggc gga gga atc  tgc ggc cca ggc att  ata gcg        3972
Thr Ser Thr  Pro Gly Gly Gly Ile  Cys Gly Pro Gly Ile  Ile Ala            
        1285                 1290                 1295                    

ggc ctg gcc  ctg ata ccg ctc ctc  ctc aag agg agg aac  tga            4014
Gly Leu Ala  Leu Ile Pro Leu Leu  Leu Lys Arg Arg Asn                     
        1300                 1305                 1310                    


<210>  2
<211>  1337
<212>  PRT
<213>  Thermococcus hydrothermalis

<400>  2

Met Arg Arg Val Val Ala Leu Phe Ile Ala Ile Leu Met Leu Gly Ser 
        -25                 -20                 -15             


Ile Val Gly Ala Asn Val Lys Ser Val Gly Ala Ala Glu Pro Lys Pro 
    -10                 -5              -1  1               5   


Leu Asn Val Ile Ile Val Trp His Gln His Gln Pro Tyr Tyr Tyr Asp 
                10                  15                  20      


Pro Val Gln Asp Val Tyr Thr Arg Pro Trp Val Arg Leu His Ala Ala 
            25                  30                  35          


Asn Asn Tyr Trp Lys Met Ala His Tyr Leu Ser Gln Tyr Pro Glu Val 
        40                  45                  50              


His Ala Thr Ile Asp Leu Ser Gly Ser Leu Ile Ala Gln Leu Ala Asp 
    55                  60                  65                  


Tyr Met Asn Gly Lys Lys Asp Thr Tyr Gln Ile Ile Thr Glu Lys Ile 
70                  75                  80                  85  


Ala Asn Gly Glu Pro Leu Thr Val Asp Glu Lys Trp Phe Met Leu Gln 
                90                  95                  100     


Ala Pro Gly Gly Phe Phe Asp Asn Thr Ile Pro Trp Asn Gly Glu Pro 
            105                 110                 115         


Ile Thr Asp Pro Asn Gly Asn Pro Ile Arg Asp Phe Trp Asp Arg Tyr 
        120                 125                 130             


Thr Glu Leu Lys Asn Lys Met Leu Ser Ala Lys Ala Lys Tyr Ala Asn 
    135                 140                 145                 


Phe Val Thr Glu Ser Gln Lys Val Ala Val Thr Asn Glu Phe Thr Glu 
150                 155                 160                 165 


Gln Asp Tyr Ile Asp Leu Ala Val Leu Phe Asn Leu Ala Trp Ile Asp 
                170                 175                 180     


Tyr Asn Tyr Ile Thr Ser Thr Pro Glu Phe Lys Ala Leu Tyr Asp Lys 
            185                 190                 195         


Val Asp Glu Gly Gly Tyr Thr Arg Ala Asp Val Lys Thr Val Leu Asp 
        200                 205                 210             


Ala Gln Ile Trp Leu Leu Asn His Thr Phe Glu Glu His Glu Lys Ile 
    215                 220                 225                 


Asn Leu Leu Leu Gly Asn Gly Asn Val Glu Val Thr Val Val Pro Tyr 
230                 235                 240                 245 


Ala His Pro Ile Gly Pro Ile Leu Asn Asp Phe Gly Trp Asp Ser Asp 
                250                 255                 260     


Phe Asn Asp Gln Val Lys Lys Ala Asp Glu Leu Tyr Lys Pro Tyr Leu 
            265                 270                 275         


Gly Gly Gly Thr Ala Val Pro Lys Gly Gly Trp Ala Ala Glu Ser Ala 
        280                 285                 290             


Leu Asn Asp Lys Thr Leu Glu Ile Leu Ala Glu Asn Gly Trp Glu Trp 
    295                 300                 305                 


Val Met Thr Asp Gln Met Val Leu Gly Lys Leu Gly Ile Glu Gly Thr 
310                 315                 320                 325 


Val Glu Asn Tyr His Lys Pro Trp Val Ala Glu Phe Asn Gly Lys Lys 
                330                 335                 340     


Ile Tyr Leu Phe Pro Arg Asn His Asp Leu Ser Asp Arg Val Gly Phe 
            345                 350                 355         


Thr Tyr Ser Gly Met Asn Gln Gln Gln Ala Val Glu Asp Phe Val Asn 
        360                 365                 370             


Glu Leu Leu Lys Leu Gln Lys Gln Asn Tyr Asp Gly Ser Leu Val Tyr 
    375                 380                 385                 


Val Val Thr Leu Asp Gly Glu Asn Pro Val Glu Asn Tyr Pro Tyr Asp 
390                 395                 400                 405 


Gly Glu Leu Phe Leu Thr Glu Leu Tyr Lys Lys Leu Thr Glu Leu Gln 
                410                 415                 420     


Glu Gln Gly Leu Ile Arg Thr Leu Thr Pro Ser Glu Tyr Ile Gln Leu 
            425                 430                 435         


Tyr Gly Asp Lys Ala Asn Lys Leu Thr Pro Arg Met Met Glu Arg Leu 
        440                 445                 450             


Asp Leu Thr Gly Asp Asn Val Asn Ala Leu Leu Lys Ala Gln Ser Leu 
    455                 460                 465                 


Gly Glu Leu Tyr Asp Met Thr Gly Val Lys Glu Glu Met Gln Trp Pro 
470                 475                 480                 485 


Glu Ser Ser Trp Ile Asp Gly Thr Leu Ser Thr Trp Ile Gly Glu Pro 
                490                 495                 500     


Gln Glu Asn Tyr Gly Trp Tyr Trp Leu Tyr Met Ala Arg Lys Ala Leu 
            505                 510                 515         


Met Glu Asn Lys Asp Lys Met Ser Gln Ala Asp Trp Glu Lys Ala Tyr 
        520                 525                 530             


Glu Tyr Leu Leu Arg Ala Glu Ala Ser Asp Trp Phe Trp Trp Tyr Gly 
    535                 540                 545                 


Ser Asp Gln Asp Ser Gly Gln Asp Tyr Thr Phe Asp Arg Tyr Leu Lys 
550                 555                 560                 565 


Thr Tyr Leu Tyr Glu Met Tyr Lys Leu Ala Gly Val Glu Pro Pro Ser 
                570                 575                 580     


Tyr Leu Phe Gly Asn Tyr Phe Pro Asp Gly Glu Pro Tyr Thr Thr Arg 
            585                 590                 595         


Gly Leu Val Gly Leu Lys Asp Gly Glu Met Lys Asn Phe Ser Ser Met 
        600                 605                 610             


Ser Pro Leu Ala Lys Gly Val Ser Val Tyr Phe Asp Gly Glu Gly Ile 
    615                 620                 625                 


His Phe Ile Val Lys Gly Asn Leu Asp Arg Phe Glu Val Ser Ile Trp 
630                 635                 640                 645 


Glu Lys Asp Glu Arg Val Gly Asn Thr Phe Thr Arg Leu Gln Glu Lys 
                650                 655                 660     


Pro Asp Glu Leu Ser Tyr Phe Met Phe Pro Phe Ser Arg Asp Ser Val 
            665                 670                 675         


Gly Leu Leu Ile Thr Lys His Val Val Tyr Glu Asn Gly Lys Ala Glu 
        680                 685                 690             


Ile Tyr Gly Ala Thr Asp Tyr Glu Lys Ser Glu Lys Leu Gly Glu Ala 
    695                 700                 705                 


Thr Val Lys Asn Thr Ser Glu Gly Ile Glu Val Val Leu Pro Phe Asp 
710                 715                 720                 725 


Tyr Ile Glu Asn Pro Ser Asp Phe Tyr Phe Ala Val Ser Thr Val Lys 
                730                 735                 740     


Asp Gly Asp Leu Glu Val Ile Ser Thr Pro Val Glu Leu Lys Leu Pro 
            745                 750                 755         


Thr Glu Val Lys Gly Val Val Ile Ala Asp Ile Thr Asp Pro Glu Gly 
        760                 765                 770             


Asp Asp His Gly Pro Gly Asn Tyr Thr Tyr Pro Thr Asp Lys Val Phe 
    775                 780                 785                 


Lys Pro Gly Val Phe Asp Leu Leu Arg Phe Arg Met Leu Glu Gln Thr 
790                 795                 800                 805 


Glu Ser Tyr Val Met Glu Phe Tyr Phe Lys Asp Leu Gly Gly Asn Pro 
                810                 815                 820     


Trp Asn Gly Pro Asn Gly Phe Ser Leu Gln Ile Ile Glu Val Tyr Leu 
            825                 830                 835         


Asp Phe Lys Asp Gly Gly Asn Ser Ser Ala Ile Lys Met Phe Pro Asp 
        840                 845                 850             


Gly Pro Gly Ala Asn Val Asn Leu Asp Pro Glu His Pro Trp Asp Val 
    855                 860                 865                 


Ala Phe Arg Ile Ala Gly Trp Asp Tyr Gly Asn Leu Ile Ile Leu Pro 
870                 875                 880                 885 


Asn Gly Thr Ala Ile Gln Gly Glu Met Gln Ile Ser Ala Asp Pro Val 
                890                 895                 900     


Lys Asn Ala Ile Ile Val Lys Val Pro Lys Lys Tyr Ile Ala Ile Asn 
            905                 910                 915         


Glu Asp Tyr Gly Leu Trp Gly Asp Val Leu Val Gly Ser Gln Asp Gly 
        920                 925                 930             


Tyr Gly Pro Asp Lys Trp Arg Thr Ala Ala Val Asp Ala Glu Gln Trp 
    935                 940                 945                 


Lys Leu Gly Gly Ala Asp Pro Gln Ala Val Ile Asn Gly Val Ala Pro 
950                 955                 960                 965 


Arg Val Ile Asp Glu Leu Val Pro Gln Gly Phe Glu Pro Thr Gln Glu 
                970                 975                 980     


Glu Gln Leu Ser Ser Tyr Asp Ala Asn Asp Met Lys Leu Ala Thr Val 
            985                 990                 995         


Lys Ala Leu  Leu Leu Leu Lys Gln  Gly Ile Val Val Thr  Asp Pro 
        1000                 1005                 1010         


Glu Gly Asp  Asp His Gly Pro Gly  Thr Tyr Thr Tyr Pro  Thr Asp 
        1015                 1020                 1025         


Lys Val Phe  Lys Pro Gly Val Phe  Asp Leu Leu Lys Phe  Lys Val 
        1030                 1035                 1040         


Thr Glu Gly  Ser Asp Asp Trp Thr  Leu Glu Phe His Phe  Lys Asp 
        1045                 1050                 1055         


Leu Gly Gly  Asn Pro Trp Asn Gly  Pro Asn Gly Phe Ser  Leu Gln 
        1060                 1065                 1070         


Ile Ile Glu  Val Tyr Phe Asp Phe  Lys Glu Gly Gly Asn  Val Ser 
        1075                 1080                 1085         


Ala Ile Lys  Met Phe Pro Asp Gly  Pro Gly Ser Asn Val  Arg Leu 
        1090                 1095                 1100         


Asp Pro Asn  His Pro Trp Asp Leu  Ala Leu Arg Ile Ala  Gly Trp 
        1105                 1110                 1115         


Asp Tyr Gly  Asn Leu Ile Ile Leu  Pro Asp Gly Thr Ala  Tyr Gln 
        1120                 1125                 1130         


Gly Glu Met  Gln Ile Ser Ala Asp  Pro Val Lys Asn Ala  Ile Ile 
        1135                 1140                 1145         


Val Lys Val  Pro Lys Lys Tyr Leu  Asn Ile Ser Asp Tyr  Gly Leu 
        1150                 1155                 1160         


Tyr Thr Ala  Val Ile Val Gly Ser  Gln Asp Gly Tyr Gly  Pro Asp 
        1165                 1170                 1175         


Lys Trp Arg  Pro Val Ala Ala Glu  Ala Glu Gln Trp Lys  Leu Gly 
        1180                 1185                 1190         


Gly Ala Asp  Pro Gln Ala Val Ile  Asp Asn Leu Val Pro  Arg Val 
        1195                 1200                 1205         


Val Asp Glu  Leu Val Pro Glu Gly  Phe Lys Pro Thr Gln  Glu Glu 
        1210                 1215                 1220         


Gln Leu Ser  Ser Tyr Asp Leu Glu  Lys Lys Thr Leu Ala  Thr Val 
        1225                 1230                 1235         


Leu Met Val  Pro Leu Val Asn Gly  Thr Gly Gly Glu Glu  Pro Thr 
        1240                 1245                 1250         


Pro Thr Glu  Ser Pro Thr Glu Thr  Thr Thr Thr Thr Pro  Ser Glu 
        1255                 1260                 1265         


Thr Thr Thr  Thr Thr Ser Thr Thr  Thr Gly Pro Ser Ser  Thr Thr 
        1270                 1275                 1280         


Thr Ser Thr  Pro Gly Gly Gly Ile  Cys Gly Pro Gly Ile  Ile Ala 
        1285                 1290                 1295         


Gly Leu Ala  Leu Ile Pro Leu Leu  Leu Lys Arg Arg Asn  
        1300                 1305                 1310 


<210>  3
<211>  3270
<212>  DNA
<213>  Thermococcus litoralis


<220>
<221>  CDS
<222>  (1)..(3267)
<223>  Mature sequence

<220>
<221>  misc_signal
<222>  (1)..(72)

<220>
<221>  mat_peptide
<222>  (73)..(3267)

<400>  3
atg aag aaa ggc ttg gca atg ttt ctc ata ttt tta gtt gcc ttg agc         48
Met Lys Lys Gly Leu Ala Met Phe Leu Ile Phe Leu Val Ala Leu Ser           
                -20                 -15                 -10               

att gct gaa gta ggg gtg aag gca gag gag cca aag cca ttg aac gtt         96
Ile Ala Glu Val Gly Val Lys Ala Glu Glu Pro Lys Pro Leu Asn Val           
            -5              -1  1               5                         

att att gtg tgg cat cag cac caa ccg tac tac tac gac cca atc cag        144
Ile Ile Val Trp His Gln His Gln Pro Tyr Tyr Tyr Asp Pro Ile Gln           
    10                  15                  20                            

gac atc tat act aga cct tgg gtt agg ctg cat gca gcc aat aat tac        192
Asp Ile Tyr Thr Arg Pro Trp Val Arg Leu His Ala Ala Asn Asn Tyr           
25                  30                  35                  40            

tgg aag atg gca aac tat ctc agc aaa tac cca gat gtt cat gtt gct        240
Trp Lys Met Ala Asn Tyr Leu Ser Lys Tyr Pro Asp Val His Val Ala           
                45                  50                  55                

ata gat ttg tcg ggt tct tta att gcc cag ctt gcc gat tac atg aac        288
Ile Asp Leu Ser Gly Ser Leu Ile Ala Gln Leu Ala Asp Tyr Met Asn           
            60                  65                  70                    

ggc aaa aaa gat aca tac cag ata gtc acg gag aaa ata gca aat ggg        336
Gly Lys Lys Asp Thr Tyr Gln Ile Val Thr Glu Lys Ile Ala Asn Gly           
        75                  80                  85                        

gaa cca cta aca ctt gaa gac aaa tgg ttc atg ctc caa gct ccg ggg        384
Glu Pro Leu Thr Leu Glu Asp Lys Trp Phe Met Leu Gln Ala Pro Gly           
    90                  95                  100                           

ggc ttt ttt gat cat act ata cct tgg aat gga gag cct gtt gca gat        432
Gly Phe Phe Asp His Thr Ile Pro Trp Asn Gly Glu Pro Val Ala Asp           
105                 110                 115                 120           

gaa aac ggc aac cct tac agg gaa caa tgg gat aga tat gca gaa ctc        480
Glu Asn Gly Asn Pro Tyr Arg Glu Gln Trp Asp Arg Tyr Ala Glu Leu           
                125                 130                 135               

aag gac aaa aga aat aat gcg ttt aaa aag tat gca aac tta cct tta        528
Lys Asp Lys Arg Asn Asn Ala Phe Lys Lys Tyr Ala Asn Leu Pro Leu           
            140                 145                 150                   

aat gag cag aag gtg aaa ata acg gct gaa ttc acg gag cag gat tac        576
Asn Glu Gln Lys Val Lys Ile Thr Ala Glu Phe Thr Glu Gln Asp Tyr           
        155                 160                 165                       

att gac tta gct gtc ttg ttc aac ttg gct tgg att gac tat aac tac        624
Ile Asp Leu Ala Val Leu Phe Asn Leu Ala Trp Ile Asp Tyr Asn Tyr           
    170                 175                 180                           

ata atc aac act cca gag ctt aag gca ctt tac gac aaa gtt gat gta        672
Ile Ile Asn Thr Pro Glu Leu Lys Ala Leu Tyr Asp Lys Val Asp Val           
185                 190                 195                 200           

ggt ggg tac acc aag gaa gat gtg gca act gtc cta aaa cac cag atg        720
Gly Gly Tyr Thr Lys Glu Asp Val Ala Thr Val Leu Lys His Gln Met           
                205                 210                 215               

tgg ctt ctc aat cac acg ttt gaa gaa cat gag aag ata aac tac ctc        768
Trp Leu Leu Asn His Thr Phe Glu Glu His Glu Lys Ile Asn Tyr Leu           
            220                 225                 230                   

ctt gga aac gga aat gtt gaa gtt act gta gtg cca tat gct cat cca        816
Leu Gly Asn Gly Asn Val Glu Val Thr Val Val Pro Tyr Ala His Pro           
        235                 240                 245                       

att ggt cca ctg ctc aac gac ttt ggc tgg tac gag gat ttt gat gct        864
Ile Gly Pro Leu Leu Asn Asp Phe Gly Trp Tyr Glu Asp Phe Asp Ala           
    250                 255                 260                           

cat gta aag aaa gcc cac gag ctc tat aag aag tat cta gga gat aac        912
His Val Lys Lys Ala His Glu Leu Tyr Lys Lys Tyr Leu Gly Asp Asn           
265                 270                 275                 280           

aga gtt gaa ccc caa gga gga tgg gct gca gaa agc gca tta aat gac        960
Arg Val Glu Pro Gln Gly Gly Trp Ala Ala Glu Ser Ala Leu Asn Asp           
                285                 290                 295               

aag acc ctt gag ata tta act aac aat gga tgg aaa tgg gta atg act       1008
Lys Thr Leu Glu Ile Leu Thr Asn Asn Gly Trp Lys Trp Val Met Thr           
            300                 305                 310                   

gat cag atg gtt ctt gac atc ctt gga att ccc aac aca gtt gaa aat       1056
Asp Gln Met Val Leu Asp Ile Leu Gly Ile Pro Asn Thr Val Glu Asn           
        315                 320                 325                       

tat tac aaa cct tgg gta gct gaa ttt aac ggc aag aaa atc tat ctc       1104
Tyr Tyr Lys Pro Trp Val Ala Glu Phe Asn Gly Lys Lys Ile Tyr Leu           
    330                 335                 340                           

ttc cca aga aac cat gac tta agt gac aga gtt ggg ttt agg tat tca       1152
Phe Pro Arg Asn His Asp Leu Ser Asp Arg Val Gly Phe Arg Tyr Ser           
345                 350                 355                 360           

ggc atg aac caa tac caa gct gtt gag gac ttt gtc aat gag ctt ctc       1200
Gly Met Asn Gln Tyr Gln Ala Val Glu Asp Phe Val Asn Glu Leu Leu           
                365                 370                 375               

aag gta caa aaa gag aac tac gat gga agc ctt gtt tat gtt gta acc       1248
Lys Val Gln Lys Glu Asn Tyr Asp Gly Ser Leu Val Tyr Val Val Thr           
            380                 385                 390                   

cta gac gga gag aac cca tgg gaa cac tac ccg ttt gat ggc aag ata       1296
Leu Asp Gly Glu Asn Pro Trp Glu His Tyr Pro Phe Asp Gly Lys Ile           
        395                 400                 405                       

ttc ctt gag gag ctg tac aag aag ctt act gaa ctt caa aag cag ggc       1344
Phe Leu Glu Glu Leu Tyr Lys Lys Leu Thr Glu Leu Gln Lys Gln Gly           
    410                 415                 420                           

tta ata agg acg gta acc ccg agt gaa tac atc cag atg tat gga gac       1392
Leu Ile Arg Thr Val Thr Pro Ser Glu Tyr Ile Gln Met Tyr Gly Asp           
425                 430                 435                 440           

aag gca aac aaa ctc act cca aag ctc atg aag agg ctt gat ttc aca       1440
Lys Ala Asn Lys Leu Thr Pro Lys Leu Met Lys Arg Leu Asp Phe Thr           
                445                 450                 455               

aca gaa gag aga gtt aat gcc cta tta aaa gct caa agc ctc ggc gag       1488
Thr Glu Glu Arg Val Asn Ala Leu Leu Lys Ala Gln Ser Leu Gly Glu           
            460                 465                 470                   

ctt tac gac atg gct ggt gtt gaa gag aat atg caa tgg cca gaa tcc       1536
Leu Tyr Asp Met Ala Gly Val Glu Glu Asn Met Gln Trp Pro Glu Ser           
        475                 480                 485                       

agt tgg gtt gat gga aca ctt tcg aca tgg att ggt gag ccc caa gag       1584
Ser Trp Val Asp Gly Thr Leu Ser Thr Trp Ile Gly Glu Pro Gln Glu           
    490                 495                 500                           

aac ttg gga tgg tac tgg ctc tac ttg gga aga aaa gca tta ttt gaa       1632
Asn Leu Gly Trp Tyr Trp Leu Tyr Leu Gly Arg Lys Ala Leu Phe Glu           
505                 510                 515                 520           

aat aag aac aag gtt gta gac tgg aac acc gca tat gaa tat ctc tta       1680
Asn Lys Asn Lys Val Val Asp Trp Asn Thr Ala Tyr Glu Tyr Leu Leu           
                525                 530                 535               

aga gca gaa gca agt gac tgg ttc tgg tgg tat gga agc gac caa gac       1728
Arg Ala Glu Ala Ser Asp Trp Phe Trp Trp Tyr Gly Ser Asp Gln Asp           
            540                 545                 550                   

agc ggg cag gac tat aca ttt gat cgc tat ctt aag acg tac ctc tat       1776
Ser Gly Gln Asp Tyr Thr Phe Asp Arg Tyr Leu Lys Thr Tyr Leu Tyr           
        555                 560                 565                       

gag atg tat aag ttc gct ggg ctg gaa att ccg agc tat ctc ttc gga       1824
Glu Met Tyr Lys Phe Ala Gly Leu Glu Ile Pro Ser Tyr Leu Phe Gly           
    570                 575                 580                           

aac tat ttc ccc aac gga gag cca tat gca ata aga gag ctc aca gga       1872
Asn Tyr Phe Pro Asn Gly Glu Pro Tyr Ala Ile Arg Glu Leu Thr Gly           
585                 590                 595                 600           

tta cca gaa gga gag aaa aag agc tgg tca agc tta tca ccc att gct       1920
Leu Pro Glu Gly Glu Lys Lys Ser Trp Ser Ser Leu Ser Pro Ile Ala           
                605                 610                 615               

gag gga gta gag ctc tac ttt gat gag cag gga tta cat ttt gtt gtt       1968
Glu Gly Val Glu Leu Tyr Phe Asp Glu Gln Gly Leu His Phe Val Val           
            620                 625                 630                   

aaa act aca aaa gag ttc gaa ata agc atc ttt gag ccc gga aag gtc       2016
Lys Thr Thr Lys Glu Phe Glu Ile Ser Ile Phe Glu Pro Gly Lys Val           
        635                 640                 645                       

atg ggt aac aca ttt act ctt ctc cag acc aaa cca agt gaa cta aga       2064
Met Gly Asn Thr Phe Thr Leu Leu Gln Thr Lys Pro Ser Glu Leu Arg           
    650                 655                 660                           

tac gat atc ttc cca ttc agc aaa gat agt gtt ggt ctt atg ata acc       2112
Tyr Asp Ile Phe Pro Phe Ser Lys Asp Ser Val Gly Leu Met Ile Thr           
665                 670                 675                 680           

aaa cat ata att gtg aaa gaa ggc aaa gca gag gtt tac aag gca aca       2160
Lys His Ile Ile Val Lys Glu Gly Lys Ala Glu Val Tyr Lys Ala Thr           
                685                 690                 695               

gac tat gaa aac agc gag aaa gtt gga gaa gtg gat gta aaa gaa acc       2208
Asp Tyr Glu Asn Ser Glu Lys Val Gly Glu Val Asp Val Lys Glu Thr           
            700                 705                 710                   

gac gga gga gtt gag gtt atc gtc ccg ttt gac tac ctg gac agc ccc       2256
Asp Gly Gly Val Glu Val Ile Val Pro Phe Asp Tyr Leu Asp Ser Pro           
        715                 720                 725                       

tct gac ttc tac ttt gct gtc tct acg gtc aat gat caa gga gag ctt       2304
Ser Asp Phe Tyr Phe Ala Val Ser Thr Val Asn Asp Gln Gly Glu Leu           
    730                 735                 740                           

gaa ata ata acg aac ccg ata gaa gta aaa ctt cca aag cag gtt gag       2352
Glu Ile Ile Thr Asn Pro Ile Glu Val Lys Leu Pro Lys Gln Val Glu           
745                 750                 755                 760           

ggg att gta gtt gca gag atc aag gac att gaa tgg gat gat cat ggt       2400
Gly Ile Val Val Ala Glu Ile Lys Asp Ile Glu Trp Asp Asp His Gly           
                765                 770                 775               

cct gga act tat acc tat gcc acc aac aag gtt ttc gtt cct gga cac       2448
Pro Gly Thr Tyr Thr Tyr Ala Thr Asn Lys Val Phe Val Pro Gly His           
            780                 785                 790                   

cta gac tta ctt aag gtg aga ata ctc gaa aag cca agt tca tac gtc       2496
Leu Asp Leu Leu Lys Val Arg Ile Leu Glu Lys Pro Ser Ser Tyr Val           
        795                 800                 805                       

ttt gag tac tac ttc aag gat ctt ggg gat aac tca tgg aat ggg cca       2544
Phe Glu Tyr Tyr Phe Lys Asp Leu Gly Asp Asn Ser Trp Asn Gly Pro           
    810                 815                 820                           

aat ggg ttc agt ttg cag ata att gag gca tac ttt gac ttc aaa gag       2592
Asn Gly Phe Ser Leu Gln Ile Ile Glu Ala Tyr Phe Asp Phe Lys Glu           
825                 830                 835                 840           

gga gga aat aca tca gca atc aaa atg ttc cct gat ggg cct gga agc       2640
Gly Gly Asn Thr Ser Ala Ile Lys Met Phe Pro Asp Gly Pro Gly Ser           
                845                 850                 855               

aac gta gac ctt gat cca gaa cat cca tgg gat gta gcc ctt aga ata       2688
Asn Val Asp Leu Asp Pro Glu His Pro Trp Asp Val Ala Leu Arg Ile           
            860                 865                 870                   

gca ggt tgg gac tat gga aac atc att gtt ctc cca gat gga aca agc       2736
Ala Gly Trp Asp Tyr Gly Asn Ile Ile Val Leu Pro Asp Gly Thr Ser           
        875                 880                 885                       

tat caa ggt gaa atg aaa atc tca gcg gat cct gtt aag aat gca att       2784
Tyr Gln Gly Glu Met Lys Ile Ser Ala Asp Pro Val Lys Asn Ala Ile           
    890                 895                 900                           

gta gta gag gtt cca aag aag tat ctt gag att agc aaa gac tat ggg       2832
Val Val Glu Val Pro Lys Lys Tyr Leu Glu Ile Ser Lys Asp Tyr Gly           
905                 910                 915                 920           

cta tat gga gcg ata tta gtg ggc tcc caa gat ggt tat gag cct gat       2880
Leu Tyr Gly Ala Ile Leu Val Gly Ser Gln Asp Gly Tyr Glu Pro Asp           
                925                 930                 935               

aag tgg aga cct gtt gca gtt gat gct gag gag tgg aag ggt ggc gga       2928
Lys Trp Arg Pro Val Ala Val Asp Ala Glu Glu Trp Lys Gly Gly Gly           
            940                 945                 950                   

gct gac gtt aat gca gtt att gct gga gtt gca cca agg gtc tat gat       2976
Ala Asp Val Asn Ala Val Ile Ala Gly Val Ala Pro Arg Val Tyr Asp           
        955                 960                 965                       

ctt tta gtc cca gag gac ttt aag cca aca caa gag gag caa cta agc       3024
Leu Leu Val Pro Glu Asp Phe Lys Pro Thr Gln Glu Glu Gln Leu Ser           
    970                 975                 980                           

agt tat gac gca gag aac gga aag aga gca ata gta aag atg ata cct       3072
Ser Tyr Asp Ala Glu Asn Gly Lys Arg Ala Ile Val Lys Met Ile Pro           
985                 990                 995                 1000          

ctg ttc gga gtt gaa  gaa aag cca agt gaa  acc gag acc ccc act         3117
Leu Phe Gly Val Glu  Glu Lys Pro Ser Glu  Thr Glu Thr Pro Thr             
                1005                 1010                 1015            

gag act gaa agt cca  aca cca agc gag act  tct tca act gta tct         3162
Glu Thr Glu Ser Pro  Thr Pro Ser Glu Thr  Ser Ser Thr Val Ser             
                1020                 1025                 1030            

cca agt tca aca agc  tct cca agc cca aca  gaa act gga gga atc         3207
Pro Ser Ser Thr Ser  Ser Pro Ser Pro Thr  Glu Thr Gly Gly Ile             
                1035                 1040                 1045            

tgc gga cca gca gca  ctc gta gga cta gca  cta atc cca cta ctc         3252
Cys Gly Pro Ala Ala  Leu Val Gly Leu Ala  Leu Ile Pro Leu Leu             
                1050                 1055                 1060            

cta aga agg agg tgg  tga                                              3270
Leu Arg Arg Arg Trp                                                       
                1065                                                      


<210>  4
<211>  1089
<212>  PRT
<213>  Thermococcus litoralis

<400>  4

Met Lys Lys Gly Leu Ala Met Phe Leu Ile Phe Leu Val Ala Leu Ser 
                -20                 -15                 -10     


Ile Ala Glu Val Gly Val Lys Ala Glu Glu Pro Lys Pro Leu Asn Val 
            -5              -1  1               5               


Ile Ile Val Trp His Gln His Gln Pro Tyr Tyr Tyr Asp Pro Ile Gln 
    10                  15                  20                  


Asp Ile Tyr Thr Arg Pro Trp Val Arg Leu His Ala Ala Asn Asn Tyr 
25                  30                  35                  40  


Trp Lys Met Ala Asn Tyr Leu Ser Lys Tyr Pro Asp Val His Val Ala 
                45                  50                  55      


Ile Asp Leu Ser Gly Ser Leu Ile Ala Gln Leu Ala Asp Tyr Met Asn 
            60                  65                  70          


Gly Lys Lys Asp Thr Tyr Gln Ile Val Thr Glu Lys Ile Ala Asn Gly 
        75                  80                  85              


Glu Pro Leu Thr Leu Glu Asp Lys Trp Phe Met Leu Gln Ala Pro Gly 
    90                  95                  100                 


Gly Phe Phe Asp His Thr Ile Pro Trp Asn Gly Glu Pro Val Ala Asp 
105                 110                 115                 120 


Glu Asn Gly Asn Pro Tyr Arg Glu Gln Trp Asp Arg Tyr Ala Glu Leu 
                125                 130                 135     


Lys Asp Lys Arg Asn Asn Ala Phe Lys Lys Tyr Ala Asn Leu Pro Leu 
            140                 145                 150         


Asn Glu Gln Lys Val Lys Ile Thr Ala Glu Phe Thr Glu Gln Asp Tyr 
        155                 160                 165             


Ile Asp Leu Ala Val Leu Phe Asn Leu Ala Trp Ile Asp Tyr Asn Tyr 
    170                 175                 180                 


Ile Ile Asn Thr Pro Glu Leu Lys Ala Leu Tyr Asp Lys Val Asp Val 
185                 190                 195                 200 


Gly Gly Tyr Thr Lys Glu Asp Val Ala Thr Val Leu Lys His Gln Met 
                205                 210                 215     


Trp Leu Leu Asn His Thr Phe Glu Glu His Glu Lys Ile Asn Tyr Leu 
            220                 225                 230         


Leu Gly Asn Gly Asn Val Glu Val Thr Val Val Pro Tyr Ala His Pro 
        235                 240                 245             


Ile Gly Pro Leu Leu Asn Asp Phe Gly Trp Tyr Glu Asp Phe Asp Ala 
    250                 255                 260                 


His Val Lys Lys Ala His Glu Leu Tyr Lys Lys Tyr Leu Gly Asp Asn 
265                 270                 275                 280 


Arg Val Glu Pro Gln Gly Gly Trp Ala Ala Glu Ser Ala Leu Asn Asp 
                285                 290                 295     


Lys Thr Leu Glu Ile Leu Thr Asn Asn Gly Trp Lys Trp Val Met Thr 
            300                 305                 310         


Asp Gln Met Val Leu Asp Ile Leu Gly Ile Pro Asn Thr Val Glu Asn 
        315                 320                 325             


Tyr Tyr Lys Pro Trp Val Ala Glu Phe Asn Gly Lys Lys Ile Tyr Leu 
    330                 335                 340                 


Phe Pro Arg Asn His Asp Leu Ser Asp Arg Val Gly Phe Arg Tyr Ser 
345                 350                 355                 360 


Gly Met Asn Gln Tyr Gln Ala Val Glu Asp Phe Val Asn Glu Leu Leu 
                365                 370                 375     


Lys Val Gln Lys Glu Asn Tyr Asp Gly Ser Leu Val Tyr Val Val Thr 
            380                 385                 390         


Leu Asp Gly Glu Asn Pro Trp Glu His Tyr Pro Phe Asp Gly Lys Ile 
        395                 400                 405             


Phe Leu Glu Glu Leu Tyr Lys Lys Leu Thr Glu Leu Gln Lys Gln Gly 
    410                 415                 420                 


Leu Ile Arg Thr Val Thr Pro Ser Glu Tyr Ile Gln Met Tyr Gly Asp 
425                 430                 435                 440 


Lys Ala Asn Lys Leu Thr Pro Lys Leu Met Lys Arg Leu Asp Phe Thr 
                445                 450                 455     


Thr Glu Glu Arg Val Asn Ala Leu Leu Lys Ala Gln Ser Leu Gly Glu 
            460                 465                 470         


Leu Tyr Asp Met Ala Gly Val Glu Glu Asn Met Gln Trp Pro Glu Ser 
        475                 480                 485             


Ser Trp Val Asp Gly Thr Leu Ser Thr Trp Ile Gly Glu Pro Gln Glu 
    490                 495                 500                 


Asn Leu Gly Trp Tyr Trp Leu Tyr Leu Gly Arg Lys Ala Leu Phe Glu 
505                 510                 515                 520 


Asn Lys Asn Lys Val Val Asp Trp Asn Thr Ala Tyr Glu Tyr Leu Leu 
                525                 530                 535     


Arg Ala Glu Ala Ser Asp Trp Phe Trp Trp Tyr Gly Ser Asp Gln Asp 
            540                 545                 550         


Ser Gly Gln Asp Tyr Thr Phe Asp Arg Tyr Leu Lys Thr Tyr Leu Tyr 
        555                 560                 565             


Glu Met Tyr Lys Phe Ala Gly Leu Glu Ile Pro Ser Tyr Leu Phe Gly 
    570                 575                 580                 


Asn Tyr Phe Pro Asn Gly Glu Pro Tyr Ala Ile Arg Glu Leu Thr Gly 
585                 590                 595                 600 


Leu Pro Glu Gly Glu Lys Lys Ser Trp Ser Ser Leu Ser Pro Ile Ala 
                605                 610                 615     


Glu Gly Val Glu Leu Tyr Phe Asp Glu Gln Gly Leu His Phe Val Val 
            620                 625                 630         


Lys Thr Thr Lys Glu Phe Glu Ile Ser Ile Phe Glu Pro Gly Lys Val 
        635                 640                 645             


Met Gly Asn Thr Phe Thr Leu Leu Gln Thr Lys Pro Ser Glu Leu Arg 
    650                 655                 660                 


Tyr Asp Ile Phe Pro Phe Ser Lys Asp Ser Val Gly Leu Met Ile Thr 
665                 670                 675                 680 


Lys His Ile Ile Val Lys Glu Gly Lys Ala Glu Val Tyr Lys Ala Thr 
                685                 690                 695     


Asp Tyr Glu Asn Ser Glu Lys Val Gly Glu Val Asp Val Lys Glu Thr 
            700                 705                 710         


Asp Gly Gly Val Glu Val Ile Val Pro Phe Asp Tyr Leu Asp Ser Pro 
        715                 720                 725             


Ser Asp Phe Tyr Phe Ala Val Ser Thr Val Asn Asp Gln Gly Glu Leu 
    730                 735                 740                 


Glu Ile Ile Thr Asn Pro Ile Glu Val Lys Leu Pro Lys Gln Val Glu 
745                 750                 755                 760 


Gly Ile Val Val Ala Glu Ile Lys Asp Ile Glu Trp Asp Asp His Gly 
                765                 770                 775     


Pro Gly Thr Tyr Thr Tyr Ala Thr Asn Lys Val Phe Val Pro Gly His 
            780                 785                 790         


Leu Asp Leu Leu Lys Val Arg Ile Leu Glu Lys Pro Ser Ser Tyr Val 
        795                 800                 805             


Phe Glu Tyr Tyr Phe Lys Asp Leu Gly Asp Asn Ser Trp Asn Gly Pro 
    810                 815                 820                 


Asn Gly Phe Ser Leu Gln Ile Ile Glu Ala Tyr Phe Asp Phe Lys Glu 
825                 830                 835                 840 


Gly Gly Asn Thr Ser Ala Ile Lys Met Phe Pro Asp Gly Pro Gly Ser 
                845                 850                 855     


Asn Val Asp Leu Asp Pro Glu His Pro Trp Asp Val Ala Leu Arg Ile 
            860                 865                 870         


Ala Gly Trp Asp Tyr Gly Asn Ile Ile Val Leu Pro Asp Gly Thr Ser 
        875                 880                 885             


Tyr Gln Gly Glu Met Lys Ile Ser Ala Asp Pro Val Lys Asn Ala Ile 
    890                 895                 900                 


Val Val Glu Val Pro Lys Lys Tyr Leu Glu Ile Ser Lys Asp Tyr Gly 
905                 910                 915                 920 


Leu Tyr Gly Ala Ile Leu Val Gly Ser Gln Asp Gly Tyr Glu Pro Asp 
                925                 930                 935     


Lys Trp Arg Pro Val Ala Val Asp Ala Glu Glu Trp Lys Gly Gly Gly 
            940                 945                 950         


Ala Asp Val Asn Ala Val Ile Ala Gly Val Ala Pro Arg Val Tyr Asp 
        955                 960                 965             


Leu Leu Val Pro Glu Asp Phe Lys Pro Thr Gln Glu Glu Gln Leu Ser 
    970                 975                 980                 


Ser Tyr Asp Ala Glu Asn Gly Lys Arg Ala Ile Val Lys Met Ile Pro  
985                 990                 995                 1000 


Leu Phe Gly Val Glu  Glu Lys Pro Ser Glu  Thr Glu Thr Pro Thr  
                1005                 1010                 1015 


Glu Thr Glu Ser Pro  Thr Pro Ser Glu Thr  Ser Ser Thr Val Ser  
                1020                 1025                 1030 


Pro Ser Ser Thr Ser  Ser Pro Ser Pro Thr  Glu Thr Gly Gly Ile  
                1035                 1040                 1045 


Cys Gly Pro Ala Ala  Leu Val Gly Leu Ala  Leu Ile Pro Leu Leu  
                1050                 1055                 1060 


Leu Arg Arg Arg Trp  
                1065 


<210>  5
<211>  59
<212>  PRT
<213>  Thermococcus hydrothermalis


<220>
<221>  MISC_FEATURE
<222>  (1)..(59)
<223>  Linker

<400>  5

Thr Pro Thr Glu Ser Pro Thr Glu Thr Thr Thr Thr Thr Pro Ser Glu 
1               5                   10                  15      


Thr Thr Thr Thr Thr Ser Thr Thr Thr Gly Pro Ser Ser Thr Thr Thr 
            20                  25                  30          


Ser Thr Pro Gly Gly Gly Ile Cys Gly Pro Gly Ile Ile Ala Gly Leu 
        35                  40                  45              


Ala Leu Ile Pro Leu Leu Leu Lys Arg Arg Asn 
    50                  55                  


<210>  6
<211>  26
<212>  PRT
<213>  Thermococcus hydrothermalis


<220>
<221>  SIGNAL
<222>  (1)..(26)

<400>  6

Met Arg Arg Val Val Ala Leu Phe Ile Ala Ile Leu Met Leu Gly Ser 
1               5                   10                  15      


Ile Val Gly Ala Asn Val Lys Ser Val Gly 
            20                  25      


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  7
cggcgtaagc ttgtttgcct                                                   20


<210>  8
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  8
aggcaaacaa gcttacgccg cgcatgatgg agcgcctt                               38


<210>  9
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  9
tgattaacgc gtttaagtat agttgccagg gccatgg                                37


<210>  10
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  10
tgattaacgc gtttaaggag gctcaacgc                                         29


<210>  11
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  11
tgattaacgc gtttagtcgt atgatgaaag ttgc                                   34


<210>  12
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<222>  (1)..(14)
<223>  Putavite Linker

<400>  12

Glu Phe His Gln His Gln His Gln His Gln His Gln His Pro 
1               5                   10                  


<210>  13
<211>  85
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Syntheric construct


<220>
<221>  SIGNAL
<222>  (1)..(85)

<400>  13

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
            20                  25                  30          


Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
        35                  40                  45              


Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
    50                  55                  60                  


Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
65                  70                  75                  80  


Ser Leu Glu Lys Arg 
                85  


<210>  14
<211>  3798
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Syntheric construct


<220>
<221>  CDS
<222>  (1)..(3798)
<223>  synthetic DNA from Thermococcus hydrothermalis for Pichia 
       pastoris expression

<220>
<221>  misc_feature
<222>  (3760)..(3795)
<223>  HQ tag

<400>  14
gct gag cca aag cct ttg aac gtc atc atc gtt tgg cat cag cac caa         48
Ala Glu Pro Lys Pro Leu Asn Val Ile Ile Val Trp His Gln His Gln           
1               5                   10                  15                

cct tac tac tac gac cca gtt caa gac gtt tac act aga cct tgg gtc         96
Pro Tyr Tyr Tyr Asp Pro Val Gln Asp Val Tyr Thr Arg Pro Trp Val           
            20                  25                  30                    

aga ttg cat gct gcc aac aac tac tgg aag atg gct cac tac ttg tct        144
Arg Leu His Ala Ala Asn Asn Tyr Trp Lys Met Ala His Tyr Leu Ser           
        35                  40                  45                        

caa tac cct gag gtt cat gct acc atc gac ttg tct ggt tct ttg atc        192
Gln Tyr Pro Glu Val His Ala Thr Ile Asp Leu Ser Gly Ser Leu Ile           
    50                  55                  60                            

gct caa ttg gcc gac tac atg aac ggt aag aag gac act tac caa atc        240
Ala Gln Leu Ala Asp Tyr Met Asn Gly Lys Lys Asp Thr Tyr Gln Ile           
65                  70                  75                  80            

atc act gag aag atc gcc aac ggt gag cct ttg act gtt gac gag aag        288
Ile Thr Glu Lys Ile Ala Asn Gly Glu Pro Leu Thr Val Asp Glu Lys           
                85                  90                  95                

tgg ttc atg ttg caa gcc cct gga ggt ttc ttc gac aac act atc cca        336
Trp Phe Met Leu Gln Ala Pro Gly Gly Phe Phe Asp Asn Thr Ile Pro           
            100                 105                 110                   

tgg aac ggt gag cct atc acc gac cca aac gga aac cct atc aga gac        384
Trp Asn Gly Glu Pro Ile Thr Asp Pro Asn Gly Asn Pro Ile Arg Asp           
        115                 120                 125                       

ttc tgg gac aga tac act gag ttg aag aac aag atg ttg tct gcc aag        432
Phe Trp Asp Arg Tyr Thr Glu Leu Lys Asn Lys Met Leu Ser Ala Lys           
    130                 135                 140                           

gcc aag tac gcc aac ttc gtc acc gag tct caa aag gtt gcc gtt acc        480
Ala Lys Tyr Ala Asn Phe Val Thr Glu Ser Gln Lys Val Ala Val Thr           
145                 150                 155                 160           

aac gag ttc acc gag cag gac tac atc gac ttg gcc gtc ttg ttc aac        528
Asn Glu Phe Thr Glu Gln Asp Tyr Ile Asp Leu Ala Val Leu Phe Asn           
                165                 170                 175               

ttg gcc tgg atc gac tac aac tac atc acc tct act cca gag ttc aag        576
Leu Ala Trp Ile Asp Tyr Asn Tyr Ile Thr Ser Thr Pro Glu Phe Lys           
            180                 185                 190                   

gca ttg tac gac aag gtt gac gag ggt gga tac aca aga gcc gac gtt        624
Ala Leu Tyr Asp Lys Val Asp Glu Gly Gly Tyr Thr Arg Ala Asp Val           
        195                 200                 205                       

aag acc gtc ttg gac gcc caa atc tgg ttg ttg aac cac acc ttc gag        672
Lys Thr Val Leu Asp Ala Gln Ile Trp Leu Leu Asn His Thr Phe Glu           
    210                 215                 220                           

gag cat gag aag atc aac ttg ttg ttg ggt aac ggt aac gtt gag gtc        720
Glu His Glu Lys Ile Asn Leu Leu Leu Gly Asn Gly Asn Val Glu Val           
225                 230                 235                 240           

aca gtt gtt cct tac gct cac cca atc gga cct atc ttg aac gac ttc        768
Thr Val Val Pro Tyr Ala His Pro Ile Gly Pro Ile Leu Asn Asp Phe           
                245                 250                 255               

ggt tgg gac tcc gac ttc aac gac cag gtc aag aag gcc gac gag ttg        816
Gly Trp Asp Ser Asp Phe Asn Asp Gln Val Lys Lys Ala Asp Glu Leu           
            260                 265                 270                   

tac aag cct tac ttg gga gga ggt aca gcc gtt cca aag gga gga tgg        864
Tyr Lys Pro Tyr Leu Gly Gly Gly Thr Ala Val Pro Lys Gly Gly Trp           
        275                 280                 285                       

gct gcc gag tct gct ttg aac gac aag act ttg gag atc ttg gct gag        912
Ala Ala Glu Ser Ala Leu Asn Asp Lys Thr Leu Glu Ile Leu Ala Glu           
    290                 295                 300                           

aac gga tgg gag tgg gtt atg acc gac cag atg gtt ttg ggt aag ttg        960
Asn Gly Trp Glu Trp Val Met Thr Asp Gln Met Val Leu Gly Lys Leu           
305                 310                 315                 320           

ggt atc gag gga acc gtt gag aac tac cat aag cct tgg gtt gca gag       1008
Gly Ile Glu Gly Thr Val Glu Asn Tyr His Lys Pro Trp Val Ala Glu           
                325                 330                 335               

ttc aac ggt aag aag atc tac ttg ttc cca aga aac cac gac ttg tca       1056
Phe Asn Gly Lys Lys Ile Tyr Leu Phe Pro Arg Asn His Asp Leu Ser           
            340                 345                 350                   

gac aga gtt gga ttc act tac tct gga atg aac caa cag caa gct gtt       1104
Asp Arg Val Gly Phe Thr Tyr Ser Gly Met Asn Gln Gln Gln Ala Val           
        355                 360                 365                       

gag gac ttc gtc aac gag ttg ttg aag ttg caa aag caa aac tac gac       1152
Glu Asp Phe Val Asn Glu Leu Leu Lys Leu Gln Lys Gln Asn Tyr Asp           
    370                 375                 380                           

ggt tcc ttg gtt tac gtt gtt act ttg gac gga gag aac cca gtc gag       1200
Gly Ser Leu Val Tyr Val Val Thr Leu Asp Gly Glu Asn Pro Val Glu           
385                 390                 395                 400           

aac tac cct tac gac ggt gag ttg ttc ttg act gag ttg tac aag aag       1248
Asn Tyr Pro Tyr Asp Gly Glu Leu Phe Leu Thr Glu Leu Tyr Lys Lys           
                405                 410                 415               

ttg aca gag ttg caa gag caa gga ttg atc aga act ttg acc cct tca       1296
Leu Thr Glu Leu Gln Glu Gln Gly Leu Ile Arg Thr Leu Thr Pro Ser           
            420                 425                 430                   

gag tac atc cag ttg tac ggt gac aag gcc aac aag ttg act cct aga       1344
Glu Tyr Ile Gln Leu Tyr Gly Asp Lys Ala Asn Lys Leu Thr Pro Arg           
        435                 440                 445                       

atg atg gag aga ttg gac ttg aca ggt gac aac gtc aac gct ttg ttg       1392
Met Met Glu Arg Leu Asp Leu Thr Gly Asp Asn Val Asn Ala Leu Leu           
    450                 455                 460                           

aag gcc cag tcc ttg ggt gag ttg tac gac atg acc gga gtc aag gag       1440
Lys Ala Gln Ser Leu Gly Glu Leu Tyr Asp Met Thr Gly Val Lys Glu           
465                 470                 475                 480           

gag atg caa tgg cca gag agt agt tgg atc gac ggt act ttg agt act       1488
Glu Met Gln Trp Pro Glu Ser Ser Trp Ile Asp Gly Thr Leu Ser Thr           
                485                 490                 495               

tgg atc ggt gag cct cag gag aac tac ggt tgg tac tgg ttg tac atg       1536
Trp Ile Gly Glu Pro Gln Glu Asn Tyr Gly Trp Tyr Trp Leu Tyr Met           
            500                 505                 510                   

gcc aga aag gcc ttg atg gag aac aag gac aag atg tca caa gcc gac       1584
Ala Arg Lys Ala Leu Met Glu Asn Lys Asp Lys Met Ser Gln Ala Asp           
        515                 520                 525                       

tgg gag aag gcc tac gag tac ttg ttg aga gcc gag gct tcc gac tgg       1632
Trp Glu Lys Ala Tyr Glu Tyr Leu Leu Arg Ala Glu Ala Ser Asp Trp           
    530                 535                 540                           

ttc tgg tgg tac ggt tcc gac caa gac tct ggt cag gac tac act ttc       1680
Phe Trp Trp Tyr Gly Ser Asp Gln Asp Ser Gly Gln Asp Tyr Thr Phe           
545                 550                 555                 560           

gac aga tac ttg aag aca tac ttg tac gag atg tac aag ttg gct gga       1728
Asp Arg Tyr Leu Lys Thr Tyr Leu Tyr Glu Met Tyr Lys Leu Ala Gly           
                565                 570                 575               

gtt gag cct cca tcc tac ttg ttc gga aac tac ttc cca gac gga gag       1776
Val Glu Pro Pro Ser Tyr Leu Phe Gly Asn Tyr Phe Pro Asp Gly Glu           
            580                 585                 590                   

cct tac aca act aga ggt ttg gtt ggt ttg aag gac gga gag atg aag       1824
Pro Tyr Thr Thr Arg Gly Leu Val Gly Leu Lys Asp Gly Glu Met Lys           
        595                 600                 605                       

aac ttc tcc agt atg tca cca ttg gcc aag ggt gtc tct gtc tac ttc       1872
Asn Phe Ser Ser Met Ser Pro Leu Ala Lys Gly Val Ser Val Tyr Phe           
    610                 615                 620                           

gac ggt gag ggt atc cat ttc atc gtt aag gga aac ttg gac aga ttc       1920
Asp Gly Glu Gly Ile His Phe Ile Val Lys Gly Asn Leu Asp Arg Phe           
625                 630                 635                 640           

gag gtc tca atc tgg gag aag gac gag aga gtt ggt aac act ttc act       1968
Glu Val Ser Ile Trp Glu Lys Asp Glu Arg Val Gly Asn Thr Phe Thr           
                645                 650                 655               

aga ttg cag gag aag cca gac gag ttg tct tac ttc atg ttc cct ttc       2016
Arg Leu Gln Glu Lys Pro Asp Glu Leu Ser Tyr Phe Met Phe Pro Phe           
            660                 665                 670                   

tcc aga gac tct gtt ggt ttg ttg atc aca aag cat gtt gtt tac gag       2064
Ser Arg Asp Ser Val Gly Leu Leu Ile Thr Lys His Val Val Tyr Glu           
        675                 680                 685                       

aac ggt aag gcc gag atc tac ggt gct acc gac tac gag aag tcc gag       2112
Asn Gly Lys Ala Glu Ile Tyr Gly Ala Thr Asp Tyr Glu Lys Ser Glu           
    690                 695                 700                           

aag ttg gga gag gcc act gtc aag aac act agt gag gga atc gag gtc       2160
Lys Leu Gly Glu Ala Thr Val Lys Asn Thr Ser Glu Gly Ile Glu Val           
705                 710                 715                 720           

gtc ttg cct ttc gac tac atc gag aac cca tcc gac ttc tac ttc gcc       2208
Val Leu Pro Phe Asp Tyr Ile Glu Asn Pro Ser Asp Phe Tyr Phe Ala           
                725                 730                 735               

gtt tcc acc gtc aag gac ggt gac ttg gag gtt atc tcc aca cct gtt       2256
Val Ser Thr Val Lys Asp Gly Asp Leu Glu Val Ile Ser Thr Pro Val           
            740                 745                 750                   

gag ttg aag ttg cct acc gag gtc aag ggt gtt gtt atc gcc gac atc       2304
Glu Leu Lys Leu Pro Thr Glu Val Lys Gly Val Val Ile Ala Asp Ile           
        755                 760                 765                       

aca gac cca gag ggt gac gac cat ggt cca ggt aac tac aca tac cca       2352
Thr Asp Pro Glu Gly Asp Asp His Gly Pro Gly Asn Tyr Thr Tyr Pro           
    770                 775                 780                           

acc gac aag gtt ttc aag cca gga gtt ttc gac ttg ttg aga ttc aga       2400
Thr Asp Lys Val Phe Lys Pro Gly Val Phe Asp Leu Leu Arg Phe Arg           
785                 790                 795                 800           

atg ttg gag caa act gag agt tac gtt atg gag ttc tac ttc aag gac       2448
Met Leu Glu Gln Thr Glu Ser Tyr Val Met Glu Phe Tyr Phe Lys Asp           
                805                 810                 815               

ttg gga ggt aac cct tgg aac ggt cca aac gga ttc tcc ttg cag atc       2496
Leu Gly Gly Asn Pro Trp Asn Gly Pro Asn Gly Phe Ser Leu Gln Ile           
            820                 825                 830                   

atc gag gtt tac ttg gac ttc aag gac gga gga aac tcc tca gcc atc       2544
Ile Glu Val Tyr Leu Asp Phe Lys Asp Gly Gly Asn Ser Ser Ala Ile           
        835                 840                 845                       

aag atg ttc cca gac gga cct gga gcc aac gtt aac ttg gac cca gag       2592
Lys Met Phe Pro Asp Gly Pro Gly Ala Asn Val Asn Leu Asp Pro Glu           
    850                 855                 860                           

cac cca tgg gac gtt gcc ttc aga att gcc ggt tgg gac tac gga aac       2640
His Pro Trp Asp Val Ala Phe Arg Ile Ala Gly Trp Asp Tyr Gly Asn           
865                 870                 875                 880           

ttg atc atc ttg cca aac gga act gcc atc caa ggt gag atg caa atc       2688
Leu Ile Ile Leu Pro Asn Gly Thr Ala Ile Gln Gly Glu Met Gln Ile           
                885                 890                 895               

tct gcc gac cct gtc aag aac gct atc atc gtt aag gtt cct aag aag       2736
Ser Ala Asp Pro Val Lys Asn Ala Ile Ile Val Lys Val Pro Lys Lys           
            900                 905                 910                   

tac atc gcc atc aac gag gac tac ggt ttg tgg ggt gac gtc ttg gtt       2784
Tyr Ile Ala Ile Asn Glu Asp Tyr Gly Leu Trp Gly Asp Val Leu Val           
        915                 920                 925                       

gga tca cag gac ggt tac gga cca gac aag tgg aga aca gct gcc gtc       2832
Gly Ser Gln Asp Gly Tyr Gly Pro Asp Lys Trp Arg Thr Ala Ala Val           
    930                 935                 940                           

gac gcc gag caa tgg aag ttg gga gga gcc gac cca caa gct gtt atc       2880
Asp Ala Glu Gln Trp Lys Leu Gly Gly Ala Asp Pro Gln Ala Val Ile           
945                 950                 955                 960           

aac gga gtt gct cct aga gtt atc gac gag ttg gtt cca cag gga ttc       2928
Asn Gly Val Ala Pro Arg Val Ile Asp Glu Leu Val Pro Gln Gly Phe           
                965                 970                 975               

gag cca aca cag gag gag caa ttg tcc tcc tac gac gcc aac gac atg       2976
Glu Pro Thr Gln Glu Glu Gln Leu Ser Ser Tyr Asp Ala Asn Asp Met           
            980                 985                 990                   

aag ttg gct acc gtc aag gca ttg  ttg ttg ttg aag caa  ggt atc gtt     3024
Lys Leu Ala Thr Val Lys Ala Leu  Leu Leu Leu Lys Gln  Gly Ile Val         
        995                 1000                 1005                     

gtt aca  gac cct gag ggt gac  gac cat gga cca gga  aca tac aca        3069
Val Thr  Asp Pro Glu Gly Asp  Asp His Gly Pro Gly  Thr Tyr Thr            
    1010                 1015                 1020                        

tac cct  acc gac aag gtt ttc  aag cca ggt gtt ttc  gac ttg ttg        3114
Tyr Pro  Thr Asp Lys Val Phe  Lys Pro Gly Val Phe  Asp Leu Leu            
    1025                 1030                 1035                        

aag ttc  aag gtt aca gag gga  agt gac gac tgg act  ttg gag ttc        3159
Lys Phe  Lys Val Thr Glu Gly  Ser Asp Asp Trp Thr  Leu Glu Phe            
    1040                 1045                 1050                        

cat ttc  aag gac ttg gga ggt  aac cct tgg aac ggt  cca aac ggt        3204
His Phe  Lys Asp Leu Gly Gly  Asn Pro Trp Asn Gly  Pro Asn Gly            
    1055                 1060                 1065                        

ttc tct  ttg cag atc atc gag  gtt tac ttc gac ttc  aag gag gga        3249
Phe Ser  Leu Gln Ile Ile Glu  Val Tyr Phe Asp Phe  Lys Glu Gly            
    1070                 1075                 1080                        

ggt aac  gtc tcc gcc atc aag  atg ttc cca gac ggt  cct gga tca        3294
Gly Asn  Val Ser Ala Ile Lys  Met Phe Pro Asp Gly  Pro Gly Ser            
    1085                 1090                 1095                        

aac gtt  aga ttg gac cca aat  cac cca tgg gac ttg  gcc ttg aga        3339
Asn Val  Arg Leu Asp Pro Asn  His Pro Trp Asp Leu  Ala Leu Arg            
    1100                 1105                 1110                        

att gcc  ggt tgg gac tac ggt  aac ttg atc atc ttg  cca gac ggt        3384
Ile Ala  Gly Trp Asp Tyr Gly  Asn Leu Ile Ile Leu  Pro Asp Gly            
    1115                 1120                 1125                        

acc gcc  tac cag ggt gag atg  caa atc tct gcc gac  cca gtt aag        3429
Thr Ala  Tyr Gln Gly Glu Met  Gln Ile Ser Ala Asp  Pro Val Lys            
    1130                 1135                 1140                        

aac gcc  atc atc gtc aag gtt  cct aag aag tac ttg  aac atc tca        3474
Asn Ala  Ile Ile Val Lys Val  Pro Lys Lys Tyr Leu  Asn Ile Ser            
    1145                 1150                 1155                        

gac tac  gga ttg tac aca gcc  gtc atc gtt gga tct  cag gac ggt        3519
Asp Tyr  Gly Leu Tyr Thr Ala  Val Ile Val Gly Ser  Gln Asp Gly            
    1160                 1165                 1170                        

tac ggt  cca gac aag tgg aga  cct gtt gct gcc gag  gct gag caa        3564
Tyr Gly  Pro Asp Lys Trp Arg  Pro Val Ala Ala Glu  Ala Glu Gln            
    1175                 1180                 1185                        

tgg aag  ttg ggt ggt gcc gac  cca caa gct gtt atc  gac aac ttg        3609
Trp Lys  Leu Gly Gly Ala Asp  Pro Gln Ala Val Ile  Asp Asn Leu            
    1190                 1195                 1200                        

gtt cca  aga gtt gtt gac gag  ttg gtt cca gag gga  ttc aag cca        3654
Val Pro  Arg Val Val Asp Glu  Leu Val Pro Glu Gly  Phe Lys Pro            
    1205                 1210                 1215                        

aca cag  gag gag caa ttg tct  tca tac gac ttg gag  aag aag act        3699
Thr Gln  Glu Glu Gln Leu Ser  Ser Tyr Asp Leu Glu  Lys Lys Thr            
    1220                 1225                 1230                        

ttg gcc  act gtt ttg atg gtt  cca ttg gtt aac gga  act ggt gga        3744
Leu Ala  Thr Val Leu Met Val  Pro Leu Val Asn Gly  Thr Gly Gly            
    1235                 1240                 1245                        

gag gag  cca gaa ttc cat cag  cac caa cat caa cac  caa cat cag        3789
Glu Glu  Pro Glu Phe His Gln  His Gln His Gln His  Gln His Gln            
    1250                 1255                 1260                        

cac cca  taa                                                          3798
His Pro                                                                   
    1265                                                                  


<210>  15
<211>  1265
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  15

Ala Glu Pro Lys Pro Leu Asn Val Ile Ile Val Trp His Gln His Gln 
1               5                   10                  15      


Pro Tyr Tyr Tyr Asp Pro Val Gln Asp Val Tyr Thr Arg Pro Trp Val 
            20                  25                  30          


Arg Leu His Ala Ala Asn Asn Tyr Trp Lys Met Ala His Tyr Leu Ser 
        35                  40                  45              


Gln Tyr Pro Glu Val His Ala Thr Ile Asp Leu Ser Gly Ser Leu Ile 
    50                  55                  60                  


Ala Gln Leu Ala Asp Tyr Met Asn Gly Lys Lys Asp Thr Tyr Gln Ile 
65                  70                  75                  80  


Ile Thr Glu Lys Ile Ala Asn Gly Glu Pro Leu Thr Val Asp Glu Lys 
                85                  90                  95      


Trp Phe Met Leu Gln Ala Pro Gly Gly Phe Phe Asp Asn Thr Ile Pro 
            100                 105                 110         


Trp Asn Gly Glu Pro Ile Thr Asp Pro Asn Gly Asn Pro Ile Arg Asp 
        115                 120                 125             


Phe Trp Asp Arg Tyr Thr Glu Leu Lys Asn Lys Met Leu Ser Ala Lys 
    130                 135                 140                 


Ala Lys Tyr Ala Asn Phe Val Thr Glu Ser Gln Lys Val Ala Val Thr 
145                 150                 155                 160 


Asn Glu Phe Thr Glu Gln Asp Tyr Ile Asp Leu Ala Val Leu Phe Asn 
                165                 170                 175     


Leu Ala Trp Ile Asp Tyr Asn Tyr Ile Thr Ser Thr Pro Glu Phe Lys 
            180                 185                 190         


Ala Leu Tyr Asp Lys Val Asp Glu Gly Gly Tyr Thr Arg Ala Asp Val 
        195                 200                 205             


Lys Thr Val Leu Asp Ala Gln Ile Trp Leu Leu Asn His Thr Phe Glu 
    210                 215                 220                 


Glu His Glu Lys Ile Asn Leu Leu Leu Gly Asn Gly Asn Val Glu Val 
225                 230                 235                 240 


Thr Val Val Pro Tyr Ala His Pro Ile Gly Pro Ile Leu Asn Asp Phe 
                245                 250                 255     


Gly Trp Asp Ser Asp Phe Asn Asp Gln Val Lys Lys Ala Asp Glu Leu 
            260                 265                 270         


Tyr Lys Pro Tyr Leu Gly Gly Gly Thr Ala Val Pro Lys Gly Gly Trp 
        275                 280                 285             


Ala Ala Glu Ser Ala Leu Asn Asp Lys Thr Leu Glu Ile Leu Ala Glu 
    290                 295                 300                 


Asn Gly Trp Glu Trp Val Met Thr Asp Gln Met Val Leu Gly Lys Leu 
305                 310                 315                 320 


Gly Ile Glu Gly Thr Val Glu Asn Tyr His Lys Pro Trp Val Ala Glu 
                325                 330                 335     


Phe Asn Gly Lys Lys Ile Tyr Leu Phe Pro Arg Asn His Asp Leu Ser 
            340                 345                 350         


Asp Arg Val Gly Phe Thr Tyr Ser Gly Met Asn Gln Gln Gln Ala Val 
        355                 360                 365             


Glu Asp Phe Val Asn Glu Leu Leu Lys Leu Gln Lys Gln Asn Tyr Asp 
    370                 375                 380                 


Gly Ser Leu Val Tyr Val Val Thr Leu Asp Gly Glu Asn Pro Val Glu 
385                 390                 395                 400 


Asn Tyr Pro Tyr Asp Gly Glu Leu Phe Leu Thr Glu Leu Tyr Lys Lys 
                405                 410                 415     


Leu Thr Glu Leu Gln Glu Gln Gly Leu Ile Arg Thr Leu Thr Pro Ser 
            420                 425                 430         


Glu Tyr Ile Gln Leu Tyr Gly Asp Lys Ala Asn Lys Leu Thr Pro Arg 
        435                 440                 445             


Met Met Glu Arg Leu Asp Leu Thr Gly Asp Asn Val Asn Ala Leu Leu 
    450                 455                 460                 


Lys Ala Gln Ser Leu Gly Glu Leu Tyr Asp Met Thr Gly Val Lys Glu 
465                 470                 475                 480 


Glu Met Gln Trp Pro Glu Ser Ser Trp Ile Asp Gly Thr Leu Ser Thr 
                485                 490                 495     


Trp Ile Gly Glu Pro Gln Glu Asn Tyr Gly Trp Tyr Trp Leu Tyr Met 
            500                 505                 510         


Ala Arg Lys Ala Leu Met Glu Asn Lys Asp Lys Met Ser Gln Ala Asp 
        515                 520                 525             


Trp Glu Lys Ala Tyr Glu Tyr Leu Leu Arg Ala Glu Ala Ser Asp Trp 
    530                 535                 540                 


Phe Trp Trp Tyr Gly Ser Asp Gln Asp Ser Gly Gln Asp Tyr Thr Phe 
545                 550                 555                 560 


Asp Arg Tyr Leu Lys Thr Tyr Leu Tyr Glu Met Tyr Lys Leu Ala Gly 
                565                 570                 575     


Val Glu Pro Pro Ser Tyr Leu Phe Gly Asn Tyr Phe Pro Asp Gly Glu 
            580                 585                 590         


Pro Tyr Thr Thr Arg Gly Leu Val Gly Leu Lys Asp Gly Glu Met Lys 
        595                 600                 605             


Asn Phe Ser Ser Met Ser Pro Leu Ala Lys Gly Val Ser Val Tyr Phe 
    610                 615                 620                 


Asp Gly Glu Gly Ile His Phe Ile Val Lys Gly Asn Leu Asp Arg Phe 
625                 630                 635                 640 


Glu Val Ser Ile Trp Glu Lys Asp Glu Arg Val Gly Asn Thr Phe Thr 
                645                 650                 655     


Arg Leu Gln Glu Lys Pro Asp Glu Leu Ser Tyr Phe Met Phe Pro Phe 
            660                 665                 670         


Ser Arg Asp Ser Val Gly Leu Leu Ile Thr Lys His Val Val Tyr Glu 
        675                 680                 685             


Asn Gly Lys Ala Glu Ile Tyr Gly Ala Thr Asp Tyr Glu Lys Ser Glu 
    690                 695                 700                 


Lys Leu Gly Glu Ala Thr Val Lys Asn Thr Ser Glu Gly Ile Glu Val 
705                 710                 715                 720 


Val Leu Pro Phe Asp Tyr Ile Glu Asn Pro Ser Asp Phe Tyr Phe Ala 
                725                 730                 735     


Val Ser Thr Val Lys Asp Gly Asp Leu Glu Val Ile Ser Thr Pro Val 
            740                 745                 750         


Glu Leu Lys Leu Pro Thr Glu Val Lys Gly Val Val Ile Ala Asp Ile 
        755                 760                 765             


Thr Asp Pro Glu Gly Asp Asp His Gly Pro Gly Asn Tyr Thr Tyr Pro 
    770                 775                 780                 


Thr Asp Lys Val Phe Lys Pro Gly Val Phe Asp Leu Leu Arg Phe Arg 
785                 790                 795                 800 


Met Leu Glu Gln Thr Glu Ser Tyr Val Met Glu Phe Tyr Phe Lys Asp 
                805                 810                 815     


Leu Gly Gly Asn Pro Trp Asn Gly Pro Asn Gly Phe Ser Leu Gln Ile 
            820                 825                 830         


Ile Glu Val Tyr Leu Asp Phe Lys Asp Gly Gly Asn Ser Ser Ala Ile 
        835                 840                 845             


Lys Met Phe Pro Asp Gly Pro Gly Ala Asn Val Asn Leu Asp Pro Glu 
    850                 855                 860                 


His Pro Trp Asp Val Ala Phe Arg Ile Ala Gly Trp Asp Tyr Gly Asn 
865                 870                 875                 880 


Leu Ile Ile Leu Pro Asn Gly Thr Ala Ile Gln Gly Glu Met Gln Ile 
                885                 890                 895     


Ser Ala Asp Pro Val Lys Asn Ala Ile Ile Val Lys Val Pro Lys Lys 
            900                 905                 910         


Tyr Ile Ala Ile Asn Glu Asp Tyr Gly Leu Trp Gly Asp Val Leu Val 
        915                 920                 925             


Gly Ser Gln Asp Gly Tyr Gly Pro Asp Lys Trp Arg Thr Ala Ala Val 
    930                 935                 940                 


Asp Ala Glu Gln Trp Lys Leu Gly Gly Ala Asp Pro Gln Ala Val Ile 
945                 950                 955                 960 


Asn Gly Val Ala Pro Arg Val Ile Asp Glu Leu Val Pro Gln Gly Phe 
                965                 970                 975     


Glu Pro Thr Gln Glu Glu Gln Leu Ser Ser Tyr Asp Ala Asn Asp Met 
            980                 985                 990         


Lys Leu Ala Thr Val Lys Ala Leu  Leu Leu Leu Lys Gln  Gly Ile Val 
        995                 1000                 1005             


Val Thr  Asp Pro Glu Gly Asp  Asp His Gly Pro Gly  Thr Tyr Thr 
    1010                 1015                 1020             


Tyr Pro  Thr Asp Lys Val Phe  Lys Pro Gly Val Phe  Asp Leu Leu 
    1025                 1030                 1035             


Lys Phe  Lys Val Thr Glu Gly  Ser Asp Asp Trp Thr  Leu Glu Phe 
    1040                 1045                 1050             


His Phe  Lys Asp Leu Gly Gly  Asn Pro Trp Asn Gly  Pro Asn Gly 
    1055                 1060                 1065             


Phe Ser  Leu Gln Ile Ile Glu  Val Tyr Phe Asp Phe  Lys Glu Gly 
    1070                 1075                 1080             


Gly Asn  Val Ser Ala Ile Lys  Met Phe Pro Asp Gly  Pro Gly Ser 
    1085                 1090                 1095             


Asn Val  Arg Leu Asp Pro Asn  His Pro Trp Asp Leu  Ala Leu Arg 
    1100                 1105                 1110             


Ile Ala  Gly Trp Asp Tyr Gly  Asn Leu Ile Ile Leu  Pro Asp Gly 
    1115                 1120                 1125             


Thr Ala  Tyr Gln Gly Glu Met  Gln Ile Ser Ala Asp  Pro Val Lys 
    1130                 1135                 1140             


Asn Ala  Ile Ile Val Lys Val  Pro Lys Lys Tyr Leu  Asn Ile Ser 
    1145                 1150                 1155             


Asp Tyr  Gly Leu Tyr Thr Ala  Val Ile Val Gly Ser  Gln Asp Gly 
    1160                 1165                 1170             


Tyr Gly  Pro Asp Lys Trp Arg  Pro Val Ala Ala Glu  Ala Glu Gln 
    1175                 1180                 1185             


Trp Lys  Leu Gly Gly Ala Asp  Pro Gln Ala Val Ile  Asp Asn Leu 
    1190                 1195                 1200             


Val Pro  Arg Val Val Asp Glu  Leu Val Pro Glu Gly  Phe Lys Pro 
    1205                 1210                 1215             


Thr Gln  Glu Glu Gln Leu Ser  Ser Tyr Asp Leu Glu  Lys Lys Thr 
    1220                 1225                 1230             


Leu Ala  Thr Val Leu Met Val  Pro Leu Val Asn Gly  Thr Gly Gly 
    1235                 1240                 1245             


Glu Glu  Pro Glu Phe His Gln  His Gln His Gln His  Gln His Gln 
    1250                 1255                 1260             


His Pro  
    1265 


<210>  16
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  16
aggggtatct ctcgagaaaa gagctgagcc aaagcctttg aacg                        44


<210>  17
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  17
ggtgctgatg gaattctggc tcctctccac cagttc                                 36


<210>  18
<211>  27
<212>  PRT
<213>  Thermococcus hydrothermalis


<220>
<221>  SIGNAL
<222>  (1)..(27)
<223>  aprH signal peptide

<400>  18

Met Lys Lys Pro Leu Gly Lys Ile Val Ala Ser Thr Ala Leu Leu Ile 
1               5                   10                  15      


Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala 
            20                  25          


<210>  19
<211>  36
<212>  PRT
<213>  Thermococcus hydrothermalis


<220>
<221>  MISC_FEATURE
<222>  (1)..(36)
<223>  aprH signal peptide incl HQ tag

<400>  19

Met Lys Lys Pro Leu Gly Lys Ile Val Ala Ser Thr Ala Leu Leu Ile 
1               5                   10                  15      


Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala His Gln His Gln His 
            20                  25                  30          


Gln His Pro Arg 
        35      


<210>  20
<211>  189
<212>  PRT
<213>  Thermococcus hydrothermalis


<220>
<221>  DOMAIN
<222>  (1)..(189)
<223>  X47 domain

<400>  20

Ser Tyr Leu Phe Gly Asn Tyr Phe Pro Asp Gly Glu Pro Tyr Thr Thr 
1               5                   10                  15      


Arg Gly Leu Val Gly Leu Lys Asp Gly Glu Met Lys Asn Phe Ser Ser 
            20                  25                  30          


Met Ser Pro Leu Ala Lys Gly Val Ser Val Tyr Phe Asp Gly Glu Gly 
        35                  40                  45              


Ile His Phe Ile Val Lys Gly Asn Leu Asp Arg Phe Glu Val Ser Ile 
    50                  55                  60                  


Trp Glu Lys Asp Glu Arg Val Gly Asn Thr Phe Thr Arg Leu Gln Glu 
65                  70                  75                  80  


Lys Pro Asp Glu Leu Ser Tyr Phe Met Phe Pro Phe Ser Arg Asp Ser 
                85                  90                  95      


Val Gly Leu Leu Ile Thr Lys His Val Val Tyr Glu Asn Gly Lys Ala 
            100                 105                 110         


Glu Ile Tyr Gly Ala Thr Asp Tyr Glu Lys Ser Glu Lys Leu Gly Glu 
        115                 120                 125             


Ala Thr Val Lys Asn Thr Ser Glu Gly Ile Glu Val Val Leu Pro Phe 
    130                 135                 140                 


Asp Tyr Ile Glu Asn Pro Ser Asp Phe Tyr Phe Ala Val Ser Thr Val 
145                 150                 155                 160 


Lys Asp Gly Asp Leu Glu Val Ile Ser Thr Pro Val Glu Leu Lys Leu 
                165                 170                 175     


Pro Thr Glu Val Lys Gly Val Val Ile Ala Asp Ile Thr 
            180                 185                 


<210>  21
<211>  189
<212>  PRT
<213>  Pyrococcus abyssi


<220>
<221>  DOMAIN
<222>  (1)..(189)
<223>  X47 domain

<400>  21

Ser Tyr Leu Phe Gly Asn Tyr Tyr Pro Asp Gly Glu Pro Tyr Ile Val 
1               5                   10                  15      


Arg Ala Leu Val Gly Leu Pro Glu Gly Val Lys Lys Asn Trp Ser Ser 
            20                  25                  30          


Leu Ser Pro Leu Ala Lys Gly Ile Glu Val Tyr Phe Asp Asp Glu Gly 
        35                  40                  45              


Leu His Phe Val Val Leu Thr Asn Arg Ser Phe Glu Ile Ser Ile Tyr 
    50                  55                  60                  


Glu Pro Glu Lys Ile Ile Gly Asn Thr Phe Thr Val Leu Gln Lys Lys 
65                  70                  75                  80  


Pro Glu Glu Phe Arg Tyr Ser Glu Val Pro Phe Ser Lys Asp Ser Val 
                85                  90                  95      


Gly Leu Leu Ile Thr Thr His Ile Thr Val Lys Gly Glu Arg Gly Glu 
            100                 105                 110         


Val Phe Lys Ala Thr Ser Tyr Asp Asn Tyr Lys Lys Val Gly Glu Val 
        115                 120                 125             


Lys Val Asn Ala Ile Asn Gly Gly Tyr Glu Val Val Val Pro Phe Asp 
    130                 135                 140                 


Tyr Ile Glu Thr Pro Ser Asp Phe Tyr Phe Ala Val Ser Thr Ile Asn 
145                 150                 155                 160 


Asp Asn Gly Ser Leu Glu Ile Ile Thr Thr Pro Ile His Leu Lys Leu 
                165                 170                 175     


Pro Lys Glu Ile Glu Gly Thr Leu Ile Thr Glu Ile Lys 
            180                 185                 


<210>  22
<211>  189
<212>  PRT
<213>  Thermococcus gammatolerans


<220>
<221>  DOMAIN
<222>  (1)..(189)
<223>  X47 domain

<400>  22

Asp Tyr Leu Tyr Gly Asn Tyr Tyr Pro Asp Gly Glu Pro Tyr Leu Arg 
1               5                   10                  15      


Arg Ala Leu Asp Gly Leu Lys Glu Gly Gln Val Arg Thr Tyr Ser Ser 
            20                  25                  30          


Leu Ser Pro Leu Ala Glu Asn Val Ser Val Tyr Phe Asp Gly Glu Gly 
        35                  40                  45              


Leu His Phe Val Leu Asn Gly Asn Leu Ser Glu Phe Glu Val Ser Leu 
    50                  55                  60                  


Tyr Glu Val Asn Arg His Val Gly Asn Thr Phe Thr Leu Leu Gln Ser 
65                  70                  75                  80  


Arg Pro Asp Glu Leu Ser Tyr Ser Thr Trp Pro Phe Ser Lys Asp Ser 
                85                  90                  95      


Val Gly Leu Met Ile Thr Lys His Ile Val Tyr Arg Asn Gly Thr Ala 
            100                 105                 110         


Glu Leu Tyr Asn Ala Thr Asp Tyr Asp Asn Ser Thr Leu Leu Gly Asn 
        115                 120                 125             


Leu Thr Val Lys Lys Thr Glu Asp Ser Val Asp Ile Thr Val Pro Phe 
    130                 135                 140                 


Asp Asn Leu Glu Ser Pro Ser Asp Phe Tyr Phe Ala Val Ser Thr Val 
145                 150                 155                 160 


Arg Asn Gly Ser Leu Glu Val Ile Ser Thr Pro Val Glu Leu Lys Leu 
                165                 170                 175     


Pro Thr Gln Val Lys Gly Ala Ile Ile Ala Asp Ile Lys 
            180                 185                 


<210>  23
<211>  189
<212>  PRT
<213>  Thermococcus sp.


<220>
<221>  DOMAIN
<222>  (1)..(189)
<223>  X47 domain

<400>  23

Asp Tyr Leu Tyr Gly Asn Tyr Tyr Pro Asp Gly Glu Pro Tyr Ile Arg 
1               5                   10                  15      


Arg Ser Leu Asp Gly Leu Lys Glu Gly Gln Val Arg Thr Tyr Ser Ser 
            20                  25                  30          


Leu Ser Pro Leu Ala Lys Asn Val Ser Val Tyr Phe Asp Gly Lys Gly 
        35                  40                  45              


Leu His Phe Val Leu Asn Gly Asn Leu Ser Glu Phe Glu Val Ser Leu 
    50                  55                  60                  


Tyr Glu Val Asn Arg Arg Val Gly Asn Thr Phe Thr Leu Leu Gln Ser 
65                  70                  75                  80  


Arg Pro Asp Glu Leu Arg Tyr Ser Thr Trp Pro Phe Ser Lys Asp Ser 
                85                  90                  95      


Val Gly Leu Met Ile Thr Lys His Ile Leu Tyr Arg Asn Gly Thr Ala 
            100                 105                 110         


Glu Ile Tyr Asn Ala Thr Gly Tyr Asp Asn Ser Thr Leu Leu Gly Asn 
        115                 120                 125             


Leu Thr Val Glu Arg Thr Gly Asp Ser Val Glu Ile Thr Val Pro Phe 
    130                 135                 140                 


Asp Tyr Ile Glu Ser Pro Ser Asp Phe Tyr Phe Ala Val Ser Thr Val 
145                 150                 155                 160 


Arg Asn Gly Ser Leu Glu Thr Ile Ser Thr Pro Val Glu Leu Lys Leu 
                165                 170                 175     


Pro Thr Gln Val Lys Gly Val Val Ile Ala Asp Ile Lys 
            180                 185                 


<210>  24
<211>  190
<212>  PRT
<213>  Pyrococcus furiosus


<220>
<221>  DOMAIN
<222>  (1)..(190)
<223>  X47 domain

<400>  24

Ser Tyr Leu Tyr Gly Asn Tyr Phe Pro Asp Gly Ala Pro Tyr Thr Val 
1               5                   10                  15      


Arg Ala Leu Glu Gly Leu Lys Glu Gly Asp Val Lys Glu Tyr Ser Ser 
            20                  25                  30          


Leu Ser Pro Val Ala Glu Gly Val Lys Val Phe Phe Asp Ser Gln Gly 
        35                  40                  45              


Leu His Phe Ile Ile Lys Gly Ser Leu Asp Lys Phe Glu Ile Ser Ile 
    50                  55                  60                  


Tyr Glu Lys Asp Lys Arg Ile Gly Asn Thr Phe Thr Leu Leu Gln Lys 
65                  70                  75                  80  


Lys Pro Asp Lys Ile Arg Tyr Asp Val Phe Pro Phe Val Arg Asp Ser 
                85                  90                  95      


Val Gly Leu Met Ile Thr Lys His Ile Val Tyr Lys Asp Gly Lys Ala 
            100                 105                 110         


Glu Ile Tyr Asn Ala Thr Asp Tyr Glu Gly Tyr Glu Lys Ile Gly Glu 
        115                 120                 125             


Ala Gln Val Ser Val Asn Gly Asp Glu Ile Glu Val Ile Val Pro Phe 
    130                 135                 140                 


Glu Tyr Leu Glu Thr Pro Glu Asp Phe Tyr Phe Ala Val Ser Thr Val 
145                 150                 155                 160 


Asp Glu Leu Gly Met Leu Glu Val Ile Thr Thr Pro Val Asn Leu Lys 
                165                 170                 175     


Leu Pro Val Gln Val Lys Gly Val Val Leu Val Asp Ile Ala 
            180                 185                 190 


<210>  25
<211>  188
<212>  PRT
<213>  Thermococcus barophilus


<220>
<221>  DOMAIN
<222>  (1)..(188)
<223>  X47 domain

<400>  25

Ser Tyr Leu Tyr Gly Asn Tyr Phe Pro Asp Gly Gln Pro Tyr Arg Val 
1               5                   10                  15      


Arg Glu Leu Ser Gly Leu Gly Glu Gly Glu Lys Lys Thr Tyr Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Ala Lys Glu Val Glu Val Tyr Phe Asp Lys Asp Gly 
        35                  40                  45              


Met His Phe Val Ile Lys Gly Ala Pro Glu Gln Phe Glu Ile Ser Ile 
    50                  55                  60                  


Tyr Glu Lys Gly Lys Ile Ile Gly Asn Thr Phe Thr Leu Leu Gln Gly 
65                  70                  75                  80  


Thr Pro Lys Tyr Glu Tyr Ser Leu Phe Pro Tyr Ile Arg Asp Ser Ile 
                85                  90                  95      


Gly Leu Met Ile Thr Lys His Val Val Tyr Lys Asp Gly Lys Ala Glu 
            100                 105                 110         


Ile Tyr Glu Ala Lys Asp Tyr Glu Thr Ser Glu Lys Val Gly Glu Ala 
        115                 120                 125             


Thr Val Glu Lys Leu Ser Asp Gly Val Glu Ile Ile Val Pro Phe Asp 
    130                 135                 140                 


Tyr Ile Glu Thr Pro Glu Asp Phe Tyr Phe Ala Val Ser Thr Val Lys 
145                 150                 155                 160 


Gly Gly Asn Leu Glu Val Ile Thr Thr Pro Val Glu Leu Arg Leu Pro 
                165                 170                 175     


Met Glu Val Lys Gly Val Pro Ile Val Asp Ile Thr 
            180                 185             


<210>  26
<211>  190
<212>  PRT
<213>  Thermococcus kodakaraensis


<220>
<221>  DOMAIN
<222>  (1)..(190)
<223>  X47 domain

<400>  26

Ser Tyr Leu Tyr Gly Asn Tyr Phe Pro Asp Gly Gln Pro Tyr Ile Thr 
1               5                   10                  15      


Arg Ala Leu Asp Gly Leu Gly Glu Gly Asp Lys Lys Glu Tyr Ser Ser 
            20                  25                  30          


Glu Ser Ala Leu Ala Lys Gly Val Glu Val Tyr Phe Glu Gly Asp Gly 
        35                  40                  45              


Ile His Phe Leu Val Lys Gly Asp Leu Asn Glu Phe Glu Val Ser Leu 
    50                  55                  60                  


Ser Ser Pro Asp Glu Arg Ile Gly Asn Thr Phe Thr Ile Leu Gln Lys 
65                  70                  75                  80  


Arg Pro Thr Glu Leu Arg Tyr Ser Leu Phe Pro Leu Ser Lys Asp Ser 
                85                  90                  95      


Val Gly Met Leu Ile Thr Thr His Val Val Tyr Lys Asp Gly Lys Ala 
            100                 105                 110         


Glu Val Tyr Lys Ala Lys Asp Tyr Glu Thr Ser Glu Lys Val Gly Asp 
        115                 120                 125             


Val Thr Ala Lys Lys Thr Asp Ala Gly Val Glu Val Val Val Pro Phe 
    130                 135                 140                 


Asp Tyr Leu Ser Asn Pro Ser Asp Phe Tyr Phe Ala Val Ser Thr Val 
145                 150                 155                 160 


Asn Glu Asn Gly Glu Leu Glu Val Ile Ser Ser Pro Val Glu Leu Lys 
                165                 170                 175     


Leu Pro Val Gln Val Lys Gly Ala Val Ile Ala Asp Ile Ala 
            180                 185                 190 


<210>  27
<211>  190
<212>  PRT
<213>  Pyrococcus furiosus


<220>
<221>  DOMAIN
<222>  (1)..(190)
<223>  X47 domain

<400>  27

Ser Tyr Leu Tyr Gly Asn Tyr Phe Pro Asp Gly Ala Pro Tyr Thr Val 
1               5                   10                  15      


Arg Ala Leu Glu Gly Leu Lys Glu Gly Asp Val Lys Glu Tyr Ser Ser 
            20                  25                  30          


Leu Ser Pro Val Ala Glu Gly Val Lys Val Phe Phe Asp Ser Gln Gly 
        35                  40                  45              


Leu His Phe Ile Ile Lys Gly Arg Ile Asp Lys Phe Glu Ile Ser Ile 
    50                  55                  60                  


Tyr Glu Lys Asp Lys Arg Ile Gly Asn Thr Phe Thr Leu Leu Gln Lys 
65                  70                  75                  80  


Lys Pro Asp Lys Ile Arg Tyr Asp Val Phe Pro Phe Val Arg Asp Ser 
                85                  90                  95      


Val Gly Leu Met Ile Thr Lys His Ile Val Tyr Lys Asp Gly Lys Ala 
            100                 105                 110         


Glu Ile Tyr Asn Ala Thr Asp Tyr Glu Gly Tyr Glu Lys Ile Gly Glu 
        115                 120                 125             


Ala Gln Val Ser Val Asn Gly Asp Glu Ile Glu Val Ile Val Pro Phe 
    130                 135                 140                 


Glu Tyr Leu Glu Thr Pro Glu Asp Phe Tyr Phe Ala Val Ser Thr Val 
145                 150                 155                 160 


Asp Glu Leu Gly Met Leu Glu Val Ile Thr Thr Pro Val Asn Leu Lys 
                165                 170                 175     


Leu Pro Val Gln Val Lys Gly Val Val Leu Val Asp Ile Ala 
            180                 185                 190 


<210>  28
<211>  189
<212>  PRT
<213>  Thermococcus onnurieus


<220>
<221>  DOMAIN
<222>  (1)..(189)
<223>  X47 domain

<400>  28

Gly Tyr Leu Tyr Gly Asn Phe Phe Pro Asp Gly Glu Pro Tyr Thr Val 
1               5                   10                  15      


Arg Ala Leu Asp Gly Leu Gly Glu Gly Gln Val Lys Asn Tyr Ser Ser 
            20                  25                  30          


Met Ser Ser Leu Ala Glu Gly Val Ser Val Tyr Phe Asp Gly Asp Gly 
        35                  40                  45              


Ile His Phe Ile Val Lys Gly Glu Leu Asn Glu Phe Glu Ile Ser Ile 
    50                  55                  60                  


Tyr Glu Lys Gly Glu Arg Val Gly Asn Thr Phe Thr Ile Leu Gln Asp 
65                  70                  75                  80  


Lys Pro Thr Glu Leu Arg Tyr Ser Met Phe Pro Phe Ser Lys Asp Ser 
                85                  90                  95      


Val Gly Leu Met Ile Thr Lys His Ile Val Tyr Lys Asp Asn Lys Ala 
            100                 105                 110         


Glu Val Tyr Gln Ala Thr Asn Tyr Glu Asp Ser Glu Lys Ile Gly Asp 
        115                 120                 125             


Ala Val Val Lys Thr Val Asn Gly Arg Val Glu Ile Ile Val Pro Phe 
    130                 135                 140                 


Glu Tyr Ile Lys Thr Pro Glu Asp Phe Tyr Phe Ala Val Ser Thr Val 
145                 150                 155                 160 


Lys Asp Gly Glu Leu Glu Val Ile Thr Thr Pro Ile Glu Leu Lys Leu 
                165                 170                 175     


Pro Thr Glu Val Lys Gly Val Thr Leu Val Asp Ile Ala 
            180                 185                 


<210>  29
<211>  189
<212>  PRT
<213>  Thermococcus sp.


<220>
<221>  DOMAIN
<222>  (1)..(189)
<223>  X47 domain

<400>  29

Ser Tyr Leu Phe Gly Asn Tyr Phe Pro Asp Gly Glu Pro Tyr Val Thr 
1               5                   10                  15      


Arg Ala Leu Asp Gly Leu Lys Glu Gly Glu Met Lys Asn Tyr Ser Ser 
            20                  25                  30          


Met Ser Pro Leu Ala Glu Gly Val Ser Val Tyr Phe Asp Gly Glu Gly 
        35                  40                  45              


Leu His Phe Ile Val Arg Gly Asn Leu Ser Gln Phe Glu Val Ser Ile 
    50                  55                  60                  


Trp Glu Lys Asp Glu Arg Val Gly Asn Thr Phe Thr Leu Leu Gln Gly 
65                  70                  75                  80  


Arg Pro Gly Glu Leu Arg Tyr Ser Met Phe Pro Phe Ser Ala Asp Ser 
                85                  90                  95      


Val Gly Leu Met Ile Thr Lys His Leu Val Tyr His Asp Gly Lys Ala 
            100                 105                 110         


Glu Val Tyr Lys Ala Thr Asp Tyr Glu Asn Ser Glu Lys Leu Gly Glu 
        115                 120                 125             


Ala Thr Val Arg Glu Thr Ser Glu Gly Ile Glu Val Val Val Pro Phe 
    130                 135                 140                 


Glu Tyr Ile Glu Asn Pro Ala Asp Phe Tyr Phe Ala Val Ser Thr Val 
145                 150                 155                 160 


Lys Asp Gly Arg Leu Glu Val Ile Ser Thr Pro Val Glu Leu Lys Leu 
                165                 170                 175     


Pro Thr Glu Val Lys Gly Val Val Ile Ala Asp Ile Ala 
            180                 185                 


<210>  30
<211>  189
<212>  PRT
<213>  Thermococcus litoralis


<220>
<221>  DOMAIN
<222>  (1)..(189)
<223>  X47 domain

<400>  30

Ser Tyr Leu Phe Gly Asn Tyr Phe Pro Asn Gly Glu Pro Tyr Ala Ile 
1               5                   10                  15      


Arg Glu Leu Thr Gly Leu Pro Glu Gly Glu Lys Lys Ser Trp Ser Ser 
            20                  25                  30          


Leu Ser Pro Ile Ala Glu Gly Val Glu Leu Tyr Phe Asp Glu Gln Gly 
        35                  40                  45              


Leu His Phe Val Val Lys Thr Thr Lys Glu Phe Glu Ile Ser Ile Phe 
    50                  55                  60                  


Glu Pro Gly Lys Val Met Gly Asn Thr Phe Thr Leu Leu Gln Thr Lys 
65                  70                  75                  80  


Pro Ser Glu Leu Arg Tyr Asp Ile Phe Pro Phe Ser Lys Asp Ser Val 
                85                  90                  95      


Gly Leu Met Ile Thr Lys His Ile Ile Val Lys Glu Gly Lys Ala Glu 
            100                 105                 110         


Val Tyr Lys Ala Thr Asp Tyr Glu Asn Ser Glu Lys Val Gly Glu Val 
        115                 120                 125             


Asp Val Lys Glu Thr Asp Gly Gly Val Glu Val Ile Val Pro Phe Asp 
    130                 135                 140                 


Tyr Leu Asp Ser Pro Ser Asp Phe Tyr Phe Ala Val Ser Thr Val Asn 
145                 150                 155                 160 


Asp Gln Gly Glu Leu Glu Ile Ile Thr Asn Pro Ile Glu Val Lys Leu 
                165                 170                 175     


Pro Lys Gln Val Glu Gly Ile Val Val Ala Glu Ile Lys 
            180                 185                 


<210>  31
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  31
gccaaggccg gttttttatg ttttacttaa ggattacgcg agcattg                     47


<210>  32
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  32
tgattaacgc gtttaagtat agttgccagg gccatgg                                37


<210>  33
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(29)
<223>  Synthetic Construct

<400>  33
tgattaacgc gtttaaggag gctcaacgc                                         29


<210>  34
<211>  809
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hybrid protein


<220>
<221>  SIGNAL
<222>  (1)..(27)

<220>
<221>  mat_peptide
<222>  (28)..(809)
<223>  Hybrid pullulanase of Thermoccus hydrothermalis and Thermococcus 
       litoralis

<400>  34

Met Lys Lys Pro Leu Gly Lys Ile Val Ala Ser Thr Ala Leu Leu Ile 
        -25                 -20                 -15             


Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala Glu Glu Pro Lys Pro 
    -10                 -5              -1  1               5   


Leu Asn Val Ile Ile Val Trp His Gln His Gln Pro Tyr Tyr Tyr Asp 
                10                  15                  20      


Pro Ile Gln Asp Ile Tyr Thr Arg Pro Trp Val Arg Leu His Ala Ala 
            25                  30                  35          


Asn Asn Tyr Trp Lys Met Ala Asn Tyr Leu Ser Lys Tyr Pro Asp Val 
        40                  45                  50              


His Val Ala Ile Asp Leu Ser Gly Ser Leu Ile Ala Gln Leu Ala Asp 
    55                  60                  65                  


Tyr Met Asn Gly Lys Lys Asp Thr Tyr Gln Ile Val Thr Glu Lys Ile 
70                  75                  80                  85  


Ala Asn Gly Glu Pro Leu Thr Leu Glu Asp Lys Trp Phe Met Leu Gln 
                90                  95                  100     


Ala Pro Gly Gly Phe Phe Asp His Thr Ile Pro Trp Asn Gly Glu Pro 
            105                 110                 115         


Val Ala Asp Glu Asn Gly Asn Pro Tyr Arg Glu Gln Trp Asp Arg Tyr 
        120                 125                 130             


Ala Glu Leu Lys Asp Lys Arg Asn Asn Ala Phe Lys Lys Tyr Ala Asn 
    135                 140                 145                 


Leu Pro Leu Asn Glu Gln Lys Val Lys Ile Thr Ala Glu Phe Thr Glu 
150                 155                 160                 165 


Gln Asp Tyr Ile Asp Leu Ala Val Leu Phe Asn Leu Ala Trp Ile Asp 
                170                 175                 180     


Tyr Asn Tyr Ile Ile Asn Thr Pro Glu Leu Lys Ala Leu Tyr Asp Lys 
            185                 190                 195         


Val Asp Val Gly Gly Tyr Thr Lys Glu Asp Val Ala Thr Val Leu Lys 
        200                 205                 210             


His Gln Met Trp Leu Leu Asn His Thr Phe Glu Glu His Glu Lys Ile 
    215                 220                 225                 


Asn Tyr Leu Leu Gly Asn Gly Asn Val Glu Val Thr Val Val Pro Tyr 
230                 235                 240                 245 


Ala His Pro Ile Gly Pro Leu Leu Asn Asp Phe Gly Trp Tyr Glu Asp 
                250                 255                 260     


Phe Asp Ala His Val Lys Lys Ala His Glu Leu Tyr Lys Lys Tyr Leu 
            265                 270                 275         


Gly Asp Asn Arg Val Glu Pro Gln Gly Gly Trp Ala Ala Glu Ser Ala 
        280                 285                 290             


Leu Asn Asp Lys Thr Leu Glu Ile Leu Thr Asn Asn Gly Trp Lys Trp 
    295                 300                 305                 


Val Met Thr Asp Gln Met Val Leu Asp Ile Leu Gly Ile Pro Asn Thr 
310                 315                 320                 325 


Val Glu Asn Tyr Tyr Lys Pro Trp Val Ala Glu Phe Asn Gly Lys Lys 
                330                 335                 340     


Ile Tyr Leu Phe Pro Arg Asn His Asp Leu Ser Asp Arg Val Gly Phe 
            345                 350                 355         


Arg Tyr Ser Gly Met Asn Gln Tyr Gln Ala Val Glu Asp Phe Val Asn 
        360                 365                 370             


Glu Leu Leu Lys Val Gln Lys Glu Asn Tyr Asp Gly Ser Leu Val Tyr 
    375                 380                 385                 


Val Val Thr Leu Asp Gly Glu Asn Pro Trp Glu His Tyr Pro Phe Asp 
390                 395                 400                 405 


Gly Lys Ile Phe Leu Glu Glu Leu Tyr Lys Lys Leu Thr Glu Leu Gln 
                410                 415                 420     


Lys Gln Gly Leu Ile Arg Thr Val Thr Pro Ser Glu Tyr Ile Gln Met 
            425                 430                 435         


Tyr Gly Asp Lys Ala Asn Lys Leu Thr Pro Arg Met Met Glu Arg Leu 
        440                 445                 450             


Asp Leu Thr Gly Asp Asn Val Asn Ala Leu Leu Lys Ala Gln Ser Leu 
    455                 460                 465                 


Gly Glu Leu Tyr Asp Met Thr Gly Val Lys Glu Glu Met Gln Trp Pro 
470                 475                 480                 485 


Glu Ser Ser Trp Ile Asp Gly Thr Leu Ser Thr Trp Ile Gly Glu Pro 
                490                 495                 500     


Gln Glu Asn Tyr Gly Trp Tyr Trp Leu Tyr Met Ala Arg Lys Ala Leu 
            505                 510                 515         


Met Glu Asn Lys Asp Lys Met Ser Gln Ala Asp Trp Glu Lys Ala Tyr 
        520                 525                 530             


Glu Tyr Leu Leu Arg Ala Glu Ala Ser Asp Trp Phe Trp Trp Tyr Gly 
    535                 540                 545                 


Ser Asp Gln Asp Ser Gly Gln Asp Tyr Thr Phe Asp Arg Tyr Leu Lys 
550                 555                 560                 565 


Thr Tyr Leu Tyr Glu Met Tyr Lys Leu Ala Gly Val Glu Pro Pro Ser 
                570                 575                 580     


Tyr Leu Phe Gly Asn Tyr Phe Pro Asp Gly Glu Pro Tyr Thr Thr Arg 
            585                 590                 595         


Gly Leu Val Gly Leu Lys Asp Gly Glu Met Lys Asn Phe Ser Ser Met 
        600                 605                 610             


Ser Pro Leu Ala Lys Gly Val Ser Val Tyr Phe Asp Gly Glu Gly Ile 
    615                 620                 625                 


His Phe Ile Val Lys Gly Asn Leu Asp Arg Phe Glu Val Ser Ile Trp 
630                 635                 640                 645 


Glu Lys Asp Glu Arg Val Gly Asn Thr Phe Thr Arg Leu Gln Glu Lys 
                650                 655                 660     


Pro Asp Glu Leu Ser Tyr Phe Met Phe Pro Phe Ser Arg Asp Ser Val 
            665                 670                 675         


Gly Leu Leu Ile Thr Lys His Val Val Tyr Glu Asn Gly Lys Ala Glu 
        680                 685                 690             


Ile Tyr Gly Ala Thr Asp Tyr Glu Lys Ser Glu Lys Leu Gly Glu Ala 
    695                 700                 705                 


Thr Val Lys Asn Thr Ser Glu Gly Ile Glu Val Val Leu Pro Phe Asp 
710                 715                 720                 725 


Tyr Ile Glu Asn Pro Ser Asp Phe Tyr Phe Ala Val Ser Thr Val Lys 
                730                 735                 740     


Asp Gly Asp Leu Glu Val Ile Ser Thr Pro Val Glu Leu Lys Leu Pro 
            745                 750                 755         


Thr Glu Val Lys Gly Val Val Ile Ala Asp Ile Thr Asp Pro Glu Gly 
        760                 765                 770             


Asp Asp His Gly Pro Gly Asn Tyr Thr 
    775                 780         


<210>  35
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (1)..(43)
<223>  C1QBX46-F Primer

<400>  35
aggggtatct ctcgagaaaa gaccatccta cttgttcgga aac                         43


<210>  36
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  36
ggtgctgatg gaattcgatg tcggcgataa caacacc                                37


<210>  37
<211>  867
<212>  DNA
<213>  Thermococcus hydrothermalis


<220>
<221>  misc_signal
<222>  (1)..(255)

<220>
<221>  CDS
<222>  (1)..(867)

<220>
<221>  misc_feature
<222>  (256)..(822)
<223>  X47 Domain

<220>
<221>  misc_feature
<222>  (829)..(864)
<223>  Histidine affinity tag for purification

<400>  37
atg aga ttt cct tca att ttt act gca gtt tta ttc gca gca tcc tcc         48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser           
1               5                   10                  15                

gca tta gct gct cca gtc aac act aca aca gaa gat gaa acg gca caa         96
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln           
            20                  25                  30                    

att ccg gct gaa gct gtc atc ggt tac tca gat tta gaa ggg gat ttc        144
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe           
        35                  40                  45                        

gat gtt gct gtt ttg cca ttt tcc aac agc aca aat aac ggg tta ttg        192
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu           
    50                  55                  60                            

ttt ata aat act act att gcc agc att gct gct aaa gaa gaa ggg gta        240
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val           
65                  70                  75                  80            

tct ctc gag aaa aga cca tcc tac ttg ttc gga aac tac ttc cca gac        288
Ser Leu Glu Lys Arg Pro Ser Tyr Leu Phe Gly Asn Tyr Phe Pro Asp           
                85                  90                  95                

gga gag cct tac aca act aga ggt ttg gtt ggt ttg aag gac gga gag        336
Gly Glu Pro Tyr Thr Thr Arg Gly Leu Val Gly Leu Lys Asp Gly Glu           
            100                 105                 110                   

atg aag aac ttc tcc agt atg tca cca ttg gcc aag ggt gtc tct gtc        384
Met Lys Asn Phe Ser Ser Met Ser Pro Leu Ala Lys Gly Val Ser Val           
        115                 120                 125                       

tac ttc gac ggt gag ggt atc cat ttc atc gtt aag gga aac ttg gac        432
Tyr Phe Asp Gly Glu Gly Ile His Phe Ile Val Lys Gly Asn Leu Asp           
    130                 135                 140                           

aga ttc gag gtc tca atc tgg gag aag gac gag aga gtt ggt aac act        480
Arg Phe Glu Val Ser Ile Trp Glu Lys Asp Glu Arg Val Gly Asn Thr           
145                 150                 155                 160           

ttc act aga ttg cag gag aag cca gac gag ttg tct tac ttc atg ttc        528
Phe Thr Arg Leu Gln Glu Lys Pro Asp Glu Leu Ser Tyr Phe Met Phe           
                165                 170                 175               

cct ttc tcc aga gac tct gtt ggt ttg ttg atc aca aag cat gtt gtt        576
Pro Phe Ser Arg Asp Ser Val Gly Leu Leu Ile Thr Lys His Val Val           
            180                 185                 190                   

tac gag aac ggt aag gcc gag atc tac ggt gct acc gac tac gag aag        624
Tyr Glu Asn Gly Lys Ala Glu Ile Tyr Gly Ala Thr Asp Tyr Glu Lys           
        195                 200                 205                       

tcc gag aag ttg gga gag gcc act gtc aag aac act agt gag gga atc        672
Ser Glu Lys Leu Gly Glu Ala Thr Val Lys Asn Thr Ser Glu Gly Ile           
    210                 215                 220                           

gag gtc gtc ttg cct ttc gac tac atc gag aac cca tcc gac ttc tac        720
Glu Val Val Leu Pro Phe Asp Tyr Ile Glu Asn Pro Ser Asp Phe Tyr           
225                 230                 235                 240           

ttc gcc gtt tcc acc gtc aag gac ggt gac ttg gag gtt atc tcc aca        768
Phe Ala Val Ser Thr Val Lys Asp Gly Asp Leu Glu Val Ile Ser Thr           
                245                 250                 255               

cct gtt gag ttg aag ttg cct acc gag gtc aag ggt gtt gtt atc gcc        816
Pro Val Glu Leu Lys Leu Pro Thr Glu Val Lys Gly Val Val Ile Ala           
            260                 265                 270                   

gac atc gaa ttc cat cag cac caa cat caa cac caa cat cag cac cca        864
Asp Ile Glu Phe His Gln His Gln His Gln His Gln His Gln His Pro           
        275                 280                 285                       

taa                                                                    867


<210>  38
<211>  288
<212>  PRT
<213>  Thermococcus hydrothermalis

<400>  38

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
            20                  25                  30          


Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
        35                  40                  45              


Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
    50                  55                  60                  


Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
65                  70                  75                  80  


Ser Leu Glu Lys Arg Pro Ser Tyr Leu Phe Gly Asn Tyr Phe Pro Asp 
                85                  90                  95      


Gly Glu Pro Tyr Thr Thr Arg Gly Leu Val Gly Leu Lys Asp Gly Glu 
            100                 105                 110         


Met Lys Asn Phe Ser Ser Met Ser Pro Leu Ala Lys Gly Val Ser Val 
        115                 120                 125             


Tyr Phe Asp Gly Glu Gly Ile His Phe Ile Val Lys Gly Asn Leu Asp 
    130                 135                 140                 


Arg Phe Glu Val Ser Ile Trp Glu Lys Asp Glu Arg Val Gly Asn Thr 
145                 150                 155                 160 


Phe Thr Arg Leu Gln Glu Lys Pro Asp Glu Leu Ser Tyr Phe Met Phe 
                165                 170                 175     


Pro Phe Ser Arg Asp Ser Val Gly Leu Leu Ile Thr Lys His Val Val 
            180                 185                 190         


Tyr Glu Asn Gly Lys Ala Glu Ile Tyr Gly Ala Thr Asp Tyr Glu Lys 
        195                 200                 205             


Ser Glu Lys Leu Gly Glu Ala Thr Val Lys Asn Thr Ser Glu Gly Ile 
    210                 215                 220                 


Glu Val Val Leu Pro Phe Asp Tyr Ile Glu Asn Pro Ser Asp Phe Tyr 
225                 230                 235                 240 


Phe Ala Val Ser Thr Val Lys Asp Gly Asp Leu Glu Val Ile Ser Thr 
                245                 250                 255     


Pro Val Glu Leu Lys Leu Pro Thr Glu Val Lys Gly Val Val Ile Ala 
            260                 265                 270         


Asp Ile Glu Phe His Gln His Gln His Gln His Gln His Gln His Pro 
        275                 280                 285             


