                         SEQUENCE LISTING

<110>  UCB Pharma S.A.
 
<120>  Bacterial Host Strain

<130>  G0121-WO

<150>  GB1000588.2
<151>  2010-01-14

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence:hTNF40 CDRH1

<400>  1

Asp Tyr Gly Met Asn 
1               5   


<210>  2
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence:hTNF40 Human hybrid CDRH2

<400>  2

Trp Ile Asn Thr Tyr Ile Gly Glu Pro Ile Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  3
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence:hTNF40 CDRH3

<400>  3

Gly Tyr Arg Ser Tyr Ala Met Asp Tyr 
1               5                   


<210>  4
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence:hTNF40 CDRL1

<400>  4

Lys Ala Ser Gln Asn Val Gly Thr Asn Val Ala 
1               5                   10      


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence:hTNF40 CDRL2

<400>  5

Ser Ala Ser Phe Leu Tyr Ser 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence:hTNF40 CDRL3

<400>  6

Gln Gln Tyr Asn Ile Tyr Pro Leu Thr 
1               5                   


<210>  7
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence:hTNF40 CDRH2

<400>  7

Trp Ile Asn Thr Tyr Ile Gly Glu Pro Ile Tyr Val Asp Asp Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  8
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence:hTNF40-gL1


<220>
<221>  CDS
<222>  (1)..(321)

<400>  8
gac att caa atg acc cag agc cca tcc agc ctg agc gca tct gta gga         48
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly           
1               5                   10                  15                

gac cgg gtc acc atc act tgt aaa gcc agt cag aac gta ggt act aac         96
Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Val Gly Thr Asn           
            20                  25                  30                    

gta gcc tgg tat cag caa aaa cca ggt aaa gcc cca aaa gcc ctc atc        144
Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Ala Leu Ile           
        35                  40                  45                        

tac agt gcc tct ttc ctc tat agt ggt gta cca tac agg ttc agc gga        192
Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Tyr Arg Phe Ser Gly           
    50                  55                  60                            

tcc ggt agt ggt act gat ttc acc ctc acg atc agt agc ctc cag cca        240
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro           
65                  70                  75                  80            

gaa gat ttc gcc act tat tac tgt caa cag tat aac atc tac cca ctc        288
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Asn Ile Tyr Pro Leu           
                85                  90                  95                

aca ttc ggt cag ggt act aaa gta gaa atc aaa                            321
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys                               
            100                 105                                       


<210>  9
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  9

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Val Gly Thr Asn 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Ala Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Tyr Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Asn Ile Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  10
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence:hTNF40-gL2


<220>
<221>  CDS
<222>  (1)..(321)

<400>  10
gac att caa atg acc cag agc cca tcc agc ctg agc gca tct gta gga         48
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly           
1               5                   10                  15                

gac cgg gtc acc atc act tgt aaa gcc agt cag aac gta ggt act aac         96
Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Val Gly Thr Asn           
            20                  25                  30                    

gta gcc tgg tat cag caa aaa cca ggt aaa gcc cca aaa ctc ctc atc        144
Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile           
        35                  40                  45                        

tac agt gcc tct ttc ctc tat agt ggt gta cca tac agg ttc agc gga        192
Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Tyr Arg Phe Ser Gly           
    50                  55                  60                            

tcc ggt agt ggt act gat ttc acc ctc acg atc agt agc ctc cag cca        240
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro           
65                  70                  75                  80            

gaa gat ttc gcc act tat tac tgt caa cag tat aac atc tac cca ctc        288
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Asn Ile Tyr Pro Leu           
                85                  90                  95                

aca ttc ggt cag ggt act aaa gta gaa atc aaa                            321
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys                               
            100                 105                                       


<210>  11
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  11

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Val Gly Thr Asn 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Tyr Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Asn Ile Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  12
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence:gh1h TNF40.4


<220>
<221>  CDS
<222>  (1)..(354)

<400>  12
cag gtg cag ctg gtc cag tca gga gca gag gtt aag aag cct ggt gct         48
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala           
1               5                   10                  15                

tcc gtc aaa gtt tcg tgt aag gcc tca ggc tac gtg ttc aca gac tat         96
Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Val Phe Thr Asp Tyr           
            20                  25                  30                    

ggt atg aat tgg gtc aga cag gcc ccg gga caa ggc ctg gaa tgg atg        144
Gly Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met           
        35                  40                  45                        

ggt tgg att aat act tac att gga gag cct att tat gct caa aag ttc        192
Gly Trp Ile Asn Thr Tyr Ile Gly Glu Pro Ile Tyr Ala Gln Lys Phe           
    50                  55                  60                            

cag ggc aga gtc acg ttc act cta gac acc tcc aca agc act gca tac        240
Gln Gly Arg Val Thr Phe Thr Leu Asp Thr Ser Thr Ser Thr Ala Tyr           
65                  70                  75                  80            

atg gag ctg tca tct ctg aga tcc gag gac acc gca gtg tac tat tgt        288
Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys           
                85                  90                  95                

gct aga gga tac aga tct tat gcc atg gac tac tgg ggc cag ggt acc        336
Ala Arg Gly Tyr Arg Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr           
            100                 105                 110                   

cta gtc aca gtc tcc tca                                                354
Leu Val Thr Val Ser Ser                                                   
        115                                                               


<210>  13
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  13

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Val Phe Thr Asp Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Ile Gly Glu Pro Ile Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Phe Thr Leu Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Tyr Arg Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  14
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence:gh3h TNF40.4


<220>
<221>  CDS
<222>  (1)..(354)

<400>  14
gag gtt cag ctg gtc gag tca gga ggc ggt ctc gtg cag cct ggc gga         48
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly           
1               5                   10                  15                

tca ctg aga ttg tcc tgt gct gca tct ggt tac gtc ttc aca gac tat         96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Val Phe Thr Asp Tyr           
            20                  25                  30                    

gga atg aat tgg gtt aga cag gcc ccg gga aag ggc ctg gaa tgg atg        144
Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Met           
        35                  40                  45                        

ggt tgg att aat act tac att gga gag cct att tat gct gac agc gtc        192
Gly Trp Ile Asn Thr Tyr Ile Gly Glu Pro Ile Tyr Ala Asp Ser Val           
    50                  55                  60                            

aag ggc aga ttc acg ttc tct cta gac aca tcc aag tca aca gca tac        240
Lys Gly Arg Phe Thr Phe Ser Leu Asp Thr Ser Lys Ser Thr Ala Tyr           
65                  70                  75                  80            

ctc caa atg aat agc ctg aga gca gag gac acc gca gtg tac tat tgt        288
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys           
                85                  90                  95                

gct aga gga tac aga tct tat gcc atg gac tac tgg ggc cag ggt acc        336
Ala Arg Gly Tyr Arg Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr           
            100                 105                 110                   

cta gtc aca gtc tcc tca                                                354
Leu Val Thr Val Ser Ser                                                   
        115                                                               


<210>  15
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  15

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Val Phe Thr Asp Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Ile Gly Glu Pro Ile Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Phe Ser Leu Asp Thr Ser Lys Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Tyr Arg Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  16
<211>  648
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Grafted Heavy Chain

<400>  16
gaggttcagc tggtcgagtc aggaggcggt ctcgtgcagc ctggcggatc actgagattg       60

tcctgtgctg catctggtta cgtcttcaca gactatggaa tgaattgggt tagacaggcc      120

ccgggaaagg gcctggaatg gatgggttgg attaatactt acattggaga gcctatttat      180

gctgacagcg tcaagggcag attcacgttc tctctagaca catccaagtc aacagcatac      240

ctccaaatga atagcctgag agcagaggac accgcagtgt actattgtgc tagaggatac      300

agatcttatg ccatggacta ctggggccag ggtaccctag tcacagtctc ctcagcttcc      360

accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca      420

gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac      480

tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc      540

tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc      600

tgcaacgtga atcacaagcc cagcaacacc aaggtcgaca agaaagtt                   648


<210>  17
<211>  216
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Grafted Heavy Chain

<400>  17

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Val Phe Thr Asp Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Ile Gly Glu Pro Ile Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Phe Ser Leu Asp Thr Ser Lys Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Tyr Arg Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Lys Val 
    210                 215     


<210>  18
<211>  642
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Grafted Light Chain

<400>  18
gacattcaaa tgacccagag cccatccagc ctgagcgcat ctgtaggaga ccgggtcacc       60

atcacttgta aagccagtca gaacgtaggt actaacgtag cctggtatca gcaaaaacca      120

ggtaaagccc caaaagccct catctacagt gcctctttcc tctatagtgg tgtaccatac      180

aggttcagcg gatccggtag tggtactgat ttcaccctca cgatcagtag cctccagcca      240

gaagatttcg ccacttatta ctgtcaacag tataacatct acccactcac attcggtcag      300

ggtactaaag tagaaatcaa acgtacggta gcggccccat ctgtcttcat cttcccgcca      360

tctgatgagc agttgaaatc tggaactgcc tctgttgtgt gcctgctgaa taacttctat      420

cccagagagg ccaaagtaca gtggaaggtg gataacgccc tccaatcggg taactcccag      480

gagagtgtca cagagcagga cagcaaggac agcacctaca gcctcagcag caccctgacg      540

ctgagcaaag cagactacga gaaacacaaa gtctacgcct gcgaagtcac ccatcagggc      600

ctgagctcac cagtaacaaa aagctttaat agaggagagt gt                         642


<210>  19
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Grafted Light Chain

<400>  19

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Val Gly Thr Asn 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Ala Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Tyr Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Asn Ile Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  20
<211>  687
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Grafted Heavy Chain

<400>  20
gaggttcagc tggtcgagtc aggaggcggt ctcgtgcagc ctggcggatc actgagattg       60

tcctgtgctg catctggtta cgtcttcaca gactatggaa tgaattgggt tagacaggcc      120

ccgggaaagg gcctggaatg gatgggttgg attaatactt acattggaga gcctatttat      180

gctgacagcg tcaagggcag attcacgttc tctctagaca catccaagtc aacagcatac      240

ctccaaatga atagcctgag agcagaggac accgcagtgt actattgtgc tagaggatac      300

agatcttatg ccatggacta ctggggccag ggtaccctag tcacagtctc ctcagcttcc      360

accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca      420

gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac      480

tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc      540

tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc      600

tgcaacgtga atcacaagcc cagcaacacc aaggtcgaca agaaagttga gcccaaatct      660

tgtgacaaaa ctcacacatg cgccgcg                                          687


<210>  21
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Grafted Heavy Chain

<400>  21

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Val Phe Thr Asp Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Ile Gly Glu Pro Ile Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Phe Ser Leu Asp Thr Ser Lys Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Tyr Arg Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr 
    210                 215                 220                 


His Thr Cys Ala Ala 
225                 


<210>  22
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OmpA oligonucleotide adaptor

<400>  22
tcgagttcta gataacgagg cgtaaaaaat gaaaaagaca gctatcgcaa ttgcagtggc       60

cttggctctg acgtacgagt cagg                                              84


<210>  23
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IGS cassette-1

<400>  23
gagctcacca gtaacaaaaa gttttaatag aggagagtgt taatgaagaa gactgctata       60

gcaattg                                                                 67


<210>  24
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IGS cassette-2

<400>  24
gagctcacca gtaacaaaaa gttttaatag aggggagtgt taaaatgaag aagactgcta       60

tagcaattg                                                               69


<210>  25
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IGS cassette-3

<400>  25
gagctcacca gtaacaaaaa gctttaatag aggagagtgt tgaggaggaa aaaaaaatga       60

agaaaactgc tatagcaatt g                                                 81


<210>  26
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IGS cassette-4

<400>  26
gagctcacca gtaacaaaaa gttttaatag aggagagtgt tgacgaggat tatataatga       60

agaaaactgc tatagcaatt g                                                 81


<210>  27
<211>  729
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutated DsbC sequence

<400>  27
atgaagaaag gttttatgtt gtttactttg ttagcggcgt tttcaggctt tgctcaggct       60

gatgacgcgg caattcaaca aacgttagcc aaaatgggca tcaaaagcag cgatattcag      120

cccgcgcctg tagctggcat gaagacagtt ctgactaaca gcggcgtgtt gtacatcacc      180

gatgatggta aacatatcat tcaggggcca atgtatgacg ttagtggcac ggctccggtc      240

aatgtcacca ataagatgct gttaaagcag ttgaatgcgc ttgaaaaaga gatgatcgtt      300

tataaagcgc cgcaggaaaa acacgtcatc accgtgttta ctgatattac ctgtggttac      360

tgccacaaac tgcatgagca aatggcagac tacaacgcgc tggggatcac cgtgcgttat      420

cttgctttcc cgcgccaggg gctggacagc gatgcagaga aagaaatgaa agctatctgg      480

tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg caggtaaaag cgtcgcacca      540

gccagttgcg acgtggatat tgccgaccat tacgcacttg gcgtccagct tggcgttagc      600

ggtactccgg cagttgtgct gagcaatggc acacttgttc cgggttacca gccgccgaaa      660

gagatgaaag aatttctcga cgaacaccaa aaaatgacca gcggtaaaca ccatcaccat      720

caccactaa                                                              729


<210>  28
<211>  242
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated DsbC sequence

<400>  28

Met Lys Lys Gly Phe Met Leu Phe Thr Leu Leu Ala Ala Phe Ser Gly 
1               5                   10                  15      


Phe Ala Gln Ala Asp Asp Ala Ala Ile Gln Gln Thr Leu Ala Lys Met 
            20                  25                  30          


Gly Ile Lys Ser Ser Asp Ile Gln Pro Ala Pro Val Ala Gly Met Lys 
        35                  40                  45              


Thr Val Leu Thr Asn Ser Gly Val Leu Tyr Ile Thr Asp Asp Gly Lys 
    50                  55                  60                  


His Ile Ile Gln Gly Pro Met Tyr Asp Val Ser Gly Thr Ala Pro Val 
65                  70                  75                  80  


Asn Val Thr Asn Lys Met Leu Leu Lys Gln Leu Asn Ala Leu Glu Lys 
                85                  90                  95      


Glu Met Ile Val Tyr Lys Ala Pro Gln Glu Lys His Val Ile Thr Val 
            100                 105                 110         


Phe Thr Asp Ile Thr Cys Gly Tyr Cys His Lys Leu His Glu Gln Met 
        115                 120                 125             


Ala Asp Tyr Asn Ala Leu Gly Ile Thr Val Arg Tyr Leu Ala Phe Pro 
    130                 135                 140                 


Arg Gln Gly Leu Asp Ser Asp Ala Glu Lys Glu Met Lys Ala Ile Trp 
145                 150                 155                 160 


Cys Ala Lys Asp Lys Asn Lys Ala Phe Asp Asp Val Met Ala Gly Lys 
                165                 170                 175     


Ser Val Ala Pro Ala Ser Cys Asp Val Asp Ile Ala Asp His Tyr Ala 
            180                 185                 190         


Leu Gly Val Gln Leu Gly Val Ser Gly Thr Pro Ala Val Val Leu Ser 
        195                 200                 205             


Asn Gly Thr Leu Val Pro Gly Tyr Gln Pro Pro Lys Glu Met Lys Glu 
    210                 215                 220                 


Phe Leu Asp Glu His Gln Lys Met Thr Ser Gly Lys His His His His 
225                 230                 235                 240 


His His 
        


