                         SEQUENCE LISTING

<110>  UCB PHARMA S.A.
 
<120>  BACTERIAL HOST STRAIN

<130>  G0104-WO

<150>  GB1000587.4
<151>  2010-01-14

<160>  37    

<170>  PatentIn version 3.5

<210>  1
<211>  2049
<212>  DNA
<213>  E. coli

<400>  1
atgaacatgt tttttaggct taccgcgtta gctggcctgc ttgcaatagc aggccagacc       60

ttcgctgtag aagatatcac gcgtgctgat caaattccgg tattaaagga agagacgcag      120

catgcgacgg taagtgagcg cgtaacgtcg cgcttcaccc gttctcatta tcgccagttc      180

gacctcgatc aggcattttc ggccaaaatc tttgaccgct acctgaatct gctcgattac      240

agccacaacg tgctgctggc aagcgatgtt gaacagttcg cgaaaaagaa aaccgagtta      300

ggcgatgaac tgcgttcagg caaactcgac gttttctacg atctctacaa tctggcgcaa      360

aagcgccgtt ttgagcgtta ccagtacgct ttgtcggtac tggaaaagcc gatggatttc      420

accggcaacg acacttataa ccttgaccgc agcaaagcgc cctggccgaa aaacgaggct      480

gagttgaacg cgctgtggga cagtaaagtc aaattcgacg agttaagcct gaagctgaca      540

ggaaaaacgg ataaagaaat tcgtgaaacc ctgactcgcc gctacaaatt tgccattcgt      600

cgtctggcgc aaaccaacag cgaagatgtt ttctcgctgg caatgacggc gtttgcgcgt      660

gaaatcgacc cgcataccaa ctatctttcc ccgcgtaata ccgaacagtt caacactgaa      720

atgagtttgt cgctggaagg tattggcgca gtgctgcaaa tggatgatga ctacaccgtt      780

atcaattcga tggtggcagg tggtccggca gcgaagagta aagctatcag cgttggtgac      840

aaaattgtcg gtgttggtca aacaggcaag ccgatggttg acgtgattgg ctggcgtctt      900

gatgatgtgg ttgccttaat taaagggccg aagggcagta aagttcgtct ggaaatttta      960

cctgctggta aagggaccaa gacccgtact gtaacgttga cccgtgaacg tattcgtctc     1020

gaagaccgcg cggttaaaat gtcggtgaag accgtcggta aagagaaagt cggcgtgctg     1080

gatattccgg gcttctatgt gggtttgaca gacgatgtca aagtgcaact gcagaaactg     1140

gaaaaacaga atgtcagcag cgtcatcatc gacctgcgta gcaatggcgg tggggcgtta     1200

actgaagccg tatcgctctc cggtctgttt attcctgcgg gtcccattgt tcaggtccgc     1260

gataacaacg gcaaggttcg tgaagatagc gataccgacg gacaggtttt ctataaaggc     1320

ccgctggtgg tgctggttga ccgcttcagt gcttcggctt cagaaatctt tgccgcggca     1380

atgcaggatt acggtcgtgc gctggttgtg ggtgaaccga cgtttggtaa aggcaccgtt     1440

cagcaatacc gttcattgaa ccgtatttac gatcagatgt tacgtcctga atggccagcg     1500

ctgggttctg tgcagtacac gatccagaaa ttctatcgcg ttaacggcgg cagtacgcaa     1560

cgtaaaggcg taacgccaga catcatcatg ccgacgggta atgaagaaac ggaaacgggt     1620

gagaaattcg aagataacgc gctgccgtgg gatagcattg atgccgcgac ttatgtgaaa     1680

tcaggagatt taacggcctt tgaaccggag ctgctgaagg aacataatgc gcgtatcgcg     1740

aaagatcctg agttccagaa catcatgaag gatatcgcgc gcttcaacgc tatgaaggac     1800

aagcgcaata tcgtttctct gaattacgct gtgcgtgaga aagagaataa tgaagatgat     1860

gcgacgcgtc tggcgcgttt gaacgaacgc tttaaacgcg aaggtaaacc ggagttgaag     1920

aaactggatg atctaccgaa agattaccag gagccggatc cttatctgga tgagacggtg     1980

aatatcgcac tcgatctggc gaagcttgaa aaagccagac ccgcggaaca acccgctccc     2040

gtcaagtaa                                                             2049


<210>  2
<211>  682
<212>  PRT
<213>  E. coli

<400>  2

Met Asn Met Phe Phe Arg Leu Thr Ala Leu Ala Gly Leu Leu Ala Ile 
1               5                   10                  15      


Ala Gly Gln Thr Phe Ala Val Glu Asp Ile Thr Arg Ala Asp Gln Ile 
            20                  25                  30          


Pro Val Leu Lys Glu Glu Thr Gln His Ala Thr Val Ser Glu Arg Val 
        35                  40                  45              


Thr Ser Arg Phe Thr Arg Ser His Tyr Arg Gln Phe Asp Leu Asp Gln 
    50                  55                  60                  


Ala Phe Ser Ala Lys Ile Phe Asp Arg Tyr Leu Asn Leu Leu Asp Tyr 
65                  70                  75                  80  


Ser His Asn Val Leu Leu Ala Ser Asp Val Glu Gln Phe Ala Lys Lys 
                85                  90                  95      


Lys Thr Glu Leu Gly Asp Glu Leu Arg Ser Gly Lys Leu Asp Val Phe 
            100                 105                 110         


Tyr Asp Leu Tyr Asn Leu Ala Gln Lys Arg Arg Phe Glu Arg Tyr Gln 
        115                 120                 125             


Tyr Ala Leu Ser Val Leu Glu Lys Pro Met Asp Phe Thr Gly Asn Asp 
    130                 135                 140                 


Thr Tyr Asn Leu Asp Arg Ser Lys Ala Pro Trp Pro Lys Asn Glu Ala 
145                 150                 155                 160 


Glu Leu Asn Ala Leu Trp Asp Ser Lys Val Lys Phe Asp Glu Leu Ser 
                165                 170                 175     


Leu Lys Leu Thr Gly Lys Thr Asp Lys Glu Ile Arg Glu Thr Leu Thr 
            180                 185                 190         


Arg Arg Tyr Lys Phe Ala Ile Arg Arg Leu Ala Gln Thr Asn Ser Glu 
        195                 200                 205             


Asp Val Phe Ser Leu Ala Met Thr Ala Phe Ala Arg Glu Ile Asp Pro 
    210                 215                 220                 


His Thr Asn Tyr Leu Ser Pro Arg Asn Thr Glu Gln Phe Asn Thr Glu 
225                 230                 235                 240 


Met Ser Leu Ser Leu Glu Gly Ile Gly Ala Val Leu Gln Met Asp Asp 
                245                 250                 255     


Asp Tyr Thr Val Ile Asn Ser Met Val Ala Gly Gly Pro Ala Ala Lys 
            260                 265                 270         


Ser Lys Ala Ile Ser Val Gly Asp Lys Ile Val Gly Val Gly Gln Thr 
        275                 280                 285             


Gly Lys Pro Met Val Asp Val Ile Gly Trp Arg Leu Asp Asp Val Val 
    290                 295                 300                 


Ala Leu Ile Lys Gly Pro Lys Gly Ser Lys Val Arg Leu Glu Ile Leu 
305                 310                 315                 320 


Pro Ala Gly Lys Gly Thr Lys Thr Arg Thr Val Thr Leu Thr Arg Glu 
                325                 330                 335     


Arg Ile Arg Leu Glu Asp Arg Ala Val Lys Met Ser Val Lys Thr Val 
            340                 345                 350         


Gly Lys Glu Lys Val Gly Val Leu Asp Ile Pro Gly Phe Tyr Val Gly 
        355                 360                 365             


Leu Thr Asp Asp Val Lys Val Gln Leu Gln Lys Leu Glu Lys Gln Asn 
    370                 375                 380                 


Val Ser Ser Val Ile Ile Asp Leu Arg Ser Asn Gly Gly Gly Ala Leu 
385                 390                 395                 400 


Thr Glu Ala Val Ser Leu Ser Gly Leu Phe Ile Pro Ala Gly Pro Ile 
                405                 410                 415     


Val Gln Val Arg Asp Asn Asn Gly Lys Val Arg Glu Asp Ser Asp Thr 
            420                 425                 430         


Asp Gly Gln Val Phe Tyr Lys Gly Pro Leu Val Val Leu Val Asp Arg 
        435                 440                 445             


Phe Ser Ala Ser Ala Ser Glu Ile Phe Ala Ala Ala Met Gln Asp Tyr 
    450                 455                 460                 


Gly Arg Ala Leu Val Val Gly Glu Pro Thr Phe Gly Lys Gly Thr Val 
465                 470                 475                 480 


Gln Gln Tyr Arg Ser Leu Asn Arg Ile Tyr Asp Gln Met Leu Arg Pro 
                485                 490                 495     


Glu Trp Pro Ala Leu Gly Ser Val Gln Tyr Thr Ile Gln Lys Phe Tyr 
            500                 505                 510         


Arg Val Asn Gly Gly Ser Thr Gln Arg Lys Gly Val Thr Pro Asp Ile 
        515                 520                 525             


Ile Met Pro Thr Gly Asn Glu Glu Thr Glu Thr Gly Glu Lys Phe Glu 
    530                 535                 540                 


Asp Asn Ala Leu Pro Trp Asp Ser Ile Asp Ala Ala Thr Tyr Val Lys 
545                 550                 555                 560 


Ser Gly Asp Leu Thr Ala Phe Glu Pro Glu Leu Leu Lys Glu His Asn 
                565                 570                 575     


Ala Arg Ile Ala Lys Asp Pro Glu Phe Gln Asn Ile Met Lys Asp Ile 
            580                 585                 590         


Ala Arg Phe Asn Ala Met Lys Asp Lys Arg Asn Ile Val Ser Leu Asn 
        595                 600                 605             


Tyr Ala Val Arg Glu Lys Glu Asn Asn Glu Asp Asp Ala Thr Arg Leu 
    610                 615                 620                 


Ala Arg Leu Asn Glu Arg Phe Lys Arg Glu Gly Lys Pro Glu Leu Lys 
625                 630                 635                 640 


Lys Leu Asp Asp Leu Pro Lys Asp Tyr Gln Glu Pro Asp Pro Tyr Leu 
                645                 650                 655     


Asp Glu Thr Val Asn Ile Ala Leu Asp Leu Ala Lys Leu Glu Lys Ala 
            660                 665                 670         


Arg Pro Ala Glu Gln Pro Ala Pro Val Lys 
        675                 680         


<210>  3
<211>  2048
<212>  DNA
<213>  E. coli

<400>  3
atgaattcgt ttttaggctt accgcgttag ctggcctgct tgcaatagca ggccagacat       60

taattgtaga agatatcacg cgtgctgatc aaattccggt attaaaggaa gagacgcagc      120

atgcgacggt aagtgagcgc gtaacgtcgc gcttcacccg ttctcattat cgccagttcg      180

acctcgatca ggcattttcg gccaaaatct ttgaccgcta cctgaatctg ctcgattaca      240

gccacaacgt gctgctggca agcgatgttg aacagttcgc gaaaaagaaa accgagttag      300

gcgatgaact gcgttcaggc aaactcgacg ttttctacga tctctacaat ctggcgcaaa      360

agcgccgttt tgagcgttac cagtacgctt tgtcggtact ggaaaagccg atggatttca      420

ccggcaacga cacttataac cttgaccgca gcaaagcgcc ctggccgaaa aacgaggctg      480

agttgaacgc gctgtgggac agtaaagtca aattcgacga gttaagcctg aagctgacag      540

gaaaaacgga taaagaaatt cgtgaaaccc tgactcgccg ctacaaattt gccattcgtc      600

gtctggcgca aaccaacagc gaagatgttt tctcgctggc aatgacggcg tttgcgcgtg      660

aaatcgaccc gcataccaac tatctttccc cgcgtaatac cgaacagttc aacactgaaa      720

tgagtttgtc gctggaaggt attggcgcag tgctgcaaat ggatgatgac tacaccgtta      780

tcaattcgat ggtggcaggt ggtccggcag cgaagagtaa agctatcagc gttggtgaca      840

aaattgtcgg tgttggtcaa acaggcaagc cgatggttga cgtgattggc tggcgtcttg      900

atgatgtggt tgccttaatt aaagggccga agggcagtaa agttcgtctg gaaattttac      960

ctgctggtaa agggaccaag acccgtactg taacgttgac ccgtgaacgt attcgtctcg     1020

aagaccgcgc ggttaaaatg tcggtgaaga ccgtcggtaa agagaaagtc ggcgtgctgg     1080

atattccggg cttctatgtg ggtttgacag acgatgtcaa agtgcaactg cagaaactgg     1140

aaaaacagaa tgtcagcagc gtcatcatcg acctgcgtag caatggcggt ggggcgttaa     1200

ctgaagccgt atcgctctcc ggtctgttta ttcctgcggg tcccattgtt caggtccgcg     1260

ataacaacgg caaggttcgt gaagatagcg ataccgacgg acaggttttc tataaaggcc     1320

cgctggtggt gctggttgac cgcttcagtg cttcggcttc agaaatcttt gccgcggcaa     1380

tgcaggatta cggtcgtgcg ctggttgtgg gtgaaccgac gtttggtaaa ggcaccgttc     1440

agcaataccg ttcattgaac cgtatttacg atcagatgtt acgtcctgaa tggccagcgc     1500

tgggttctgt gcagtacacg atccagaaat tctatcgcgt taacggcggc agtacgcaac     1560

gtaaaggcgt aacgccagac atcatcatgc cgacgggtaa tgaagaaacg gaaacgggtg     1620

agaaattcga agataacgcg ctgccgtggg atagcattga tgccgcgact tatgtgaaat     1680

caggagattt aacggccttt gaaccggagc tgctgaagga acataatgcg cgtatcgcga     1740

aagatcctga gttccagaac atcatgaagg atatcgcgcg cttcaacgct atgaaggaca     1800

agcgcaatat cgtttctctg aattacgctg tgcgtgagaa agagaataat gaagatgatg     1860

cgacgcgtct ggcgcgtttg aacgaacgct ttaaacgcga aggtaaaccg gagttgaaga     1920

aactggatga tctaccgaaa gattaccagg agccggatcc ttatctggat gagacggtga     1980

atatcgcact cgatctggcg aagcttgaaa aagccagacc cgcggaacaa cccgctcccg     2040

tcaagtaa                                                              2048


<210>  4
<211>  2889
<212>  DNA
<213>  E. coli

<400>  4
atgccccgca gcacctggtt caaagcatta ttgttgttag ttgccctttg ggcaccctta       60

agtcaggcag aaacgggatg gcagccgatt caggaaacca tccgtaaaag tgataaagat      120

aaccgccagt atcaggctat acgtctggat aacggtatgg tggtcttgct ggtttctgat      180

ccgcaggcag ttaaatcgct ctcggcgctg gtggtgcccg ttgggtcgct ggaagatccc      240

gaggcgtacc aggggctggc acattacctt gaacatatga gtctgatggg gtcgaaaaag      300

tacccgcagg ctgacagtct ggccgaatat ctcaaaatgc acggcggtag tcacaatgcc      360

agcactgcgc cgtatcgcac ggctttctat ctggaagttg agaacgacgc cttgcctggt      420

gcggtagacc gcctggccga tgctattgct gaacctttgc tcgacaagaa atatgccgaa      480

cgtgagcgta atgcggtgaa cgctgaatta accatggcgc gtacgcgtga cgggatgcgc      540

atggcacagg tcagcgcaga aaccattaac ccggcacacc ccggttcaaa gttttctggt      600

ggtaacctcg aaactttaag cgacaaacct ggtaatccgg tgcagcaggc gctgaaagat      660

ttccacgaga agtactattc cgccaatttg atgaaggcgg ttatttacag taataaaccg      720

ctgccggagt tggcaaaaat ggcggcggac acctttggtc gcgtgccgaa caaagagagc      780

aaaaaaccgg aaatcaccgt gccggtagtc accgacgcgc aaaagggcat tatcattcat      840

tacgtccctg cgctgccgcg taaagtgttg cgcgttgagt ttcgcatcga taacaactca      900

gcgaagttcc gtagtaaaac cgatgaattg attacctatc tgattggcaa tcgcagccca      960

ggtacacttt ctgactggct gcaaaagcag ggattagttg agggcattag cgccaactcc     1020

gatcctatcg tcaacggcaa cagcggcgta ttagcgatct ctgcgtcttt aaccgataaa     1080

ggcctggcta atcgcgatca ggttgtggcg gcaattttta gctatctcaa tctgttacgt     1140

gaaaaaggca ttgataaaca atacttcgat gaactggcga atgtgctgga tatcgacttc     1200

cgttatccgt cgatcacccg tgatatggat tacgtcgaat ggctggcaga taccatgatt     1260

cgcgttcctg ttgagcatac gctggatgca gtcaatattg ccgatcggta cgatgctaaa     1320

gcagtaaagg aacgtctggc gatgatgacg ccgcagaatg cgcgtatctg gtatatcagc     1380

ccgaaagagc cgcacaacaa aacggcttac tttgtcgatg cgccgtatca ggtcgataaa     1440

atcagcgcac aaactttcgc cgactggcag aaaaaagccg ccgacattgc gctctctttg     1500

ccagagctta acccttatat tcctgatgat ttctcgctga ttaagtcaga gaagaaatac     1560

gaccatccag agctgattgt tgatgagtcg aatctgcgcg tggtgtatgc gccaagccgt     1620

tattttgcca gcgagcccaa agctgatgtc agcctgattt tgcgtaatcc gaaagccatg     1680

gacagcgccc gcaatcaggt gatgtttgcg ctcaatgatt atctcgcagg gctggcgctt     1740

gatcagttaa gcaaccaggc gtcggttggt ggcataagtt tttccaccaa cgctaacaac     1800

ggccttatgg ttaatgctaa tggttacacc cagcgtctgc cgcagctgtt ccaggcattg     1860

ctcgaggggt actttagcta taccgctacg gaagatcagc ttgagcaggc gaagtcctgg     1920

tataaccaga tgatggattc cgcagaaaag ggtaaagcgt ttgagcaggc gattatgccc     1980

gcgcagatgc tctcgcaagt gccgtacttc tcgcgagatg aacggcgtaa aattttgccc     2040

tccattacgt tgaaagaggt gctggcctat cgcgacgcct taaaatcagg ggctcgacca     2100

gagtttatgg ttatcggcaa catgaccgag gcccaggcaa caacgctggc acgcgatgtg     2160

caaaaacagt tgggcgctga tggttcagag tggtgtcgaa acaaagatgt agtggtcgat     2220

aaaaaacaat ccgtcatctt tgaaaaagcc ggtaacagca ccgactccgc actggcagcg     2280

gtatttgtac cgactggcta cgatgaatac accagctcag cctatagctc tctgttgggg     2340

cagatcgtac agccgtggtt ctacaatcag ttgcgtaccg aagaacaatt gggctatgcc     2400

gtgtttgcgt ttccaatgag cgtggggcgt cagtggggca tgggcttcct tttgcaaagc     2460

aatgataaac agccttcatt cttgtgggag cgttacaagg cgtttttccc aaccgcagag     2520

gcaaaattgc gagcgatgaa gccagatgag tttgcgcaaa tccagcaggc ggtaattacc     2580

cagatgctgc aggcaccgca aacgctcggc gaagaagcat cgaagttaag taaagatttc     2640

gatcgcggca atatgcgctt cgattcgcgt gataaaatcg tggcccagat aaaactgctg     2700

acgccgcaaa aacttgctga tttcttccat caggcggtgg tcgagccgca aggcatggct     2760

attctgtcgc agatttccgg cagccagaac gggaaagccg aatatgtaca ccctgaaggc     2820

tggaaagtgt gggagaacgt cagcgcgttg cagcaaacaa tgcccctgat gagtgaaaag     2880

aatgagtga                                                             2889


<210>  5
<211>  962
<212>  PRT
<213>  E. coli

<400>  5

Met Pro Arg Ser Thr Trp Phe Lys Ala Leu Leu Leu Leu Val Ala Leu 
1               5                   10                  15      


Trp Ala Pro Leu Ser Gln Ala Glu Thr Gly Trp Gln Pro Ile Gln Glu 
            20                  25                  30          


Thr Ile Arg Lys Ser Asp Lys Asp Asn Arg Gln Tyr Gln Ala Ile Arg 
        35                  40                  45              


Leu Asp Asn Gly Met Val Val Leu Leu Val Ser Asp Pro Gln Ala Val 
    50                  55                  60                  


Lys Ser Leu Ser Ala Leu Val Val Pro Val Gly Ser Leu Glu Asp Pro 
65                  70                  75                  80  


Glu Ala Tyr Gln Gly Leu Ala His Tyr Leu Glu His Met Ser Leu Met 
                85                  90                  95      


Gly Ser Lys Lys Tyr Pro Gln Ala Asp Ser Leu Ala Glu Tyr Leu Lys 
            100                 105                 110         


Met His Gly Gly Ser His Asn Ala Ser Thr Ala Pro Tyr Arg Thr Ala 
        115                 120                 125             


Phe Tyr Leu Glu Val Glu Asn Asp Ala Leu Pro Gly Ala Val Asp Arg 
    130                 135                 140                 


Leu Ala Asp Ala Ile Ala Glu Pro Leu Leu Asp Lys Lys Tyr Ala Glu 
145                 150                 155                 160 


Arg Glu Arg Asn Ala Val Asn Ala Glu Leu Thr Met Ala Arg Thr Arg 
                165                 170                 175     


Asp Gly Met Arg Met Ala Gln Val Ser Ala Glu Thr Ile Asn Pro Ala 
            180                 185                 190         


His Pro Gly Ser Lys Phe Ser Gly Gly Asn Leu Glu Thr Leu Ser Asp 
        195                 200                 205             


Lys Pro Gly Asn Pro Val Gln Gln Ala Leu Lys Asp Phe His Glu Lys 
    210                 215                 220                 


Tyr Tyr Ser Ala Asn Leu Met Lys Ala Val Ile Tyr Ser Asn Lys Pro 
225                 230                 235                 240 


Leu Pro Glu Leu Ala Lys Met Ala Ala Asp Thr Phe Gly Arg Val Pro 
                245                 250                 255     


Asn Lys Glu Ser Lys Lys Pro Glu Ile Thr Val Pro Val Val Thr Asp 
            260                 265                 270         


Ala Gln Lys Gly Ile Ile Ile His Tyr Val Pro Ala Leu Pro Arg Lys 
        275                 280                 285             


Val Leu Arg Val Glu Phe Arg Ile Asp Asn Asn Ser Ala Lys Phe Arg 
    290                 295                 300                 


Ser Lys Thr Asp Glu Leu Ile Thr Tyr Leu Ile Gly Asn Arg Ser Pro 
305                 310                 315                 320 


Gly Thr Leu Ser Asp Trp Leu Gln Lys Gln Gly Leu Val Glu Gly Ile 
                325                 330                 335     


Ser Ala Asn Ser Asp Pro Ile Val Asn Gly Asn Ser Gly Val Leu Ala 
            340                 345                 350         


Ile Ser Ala Ser Leu Thr Asp Lys Gly Leu Ala Asn Arg Asp Gln Val 
        355                 360                 365             


Val Ala Ala Ile Phe Ser Tyr Leu Asn Leu Leu Arg Glu Lys Gly Ile 
    370                 375                 380                 


Asp Lys Gln Tyr Phe Asp Glu Leu Ala Asn Val Leu Asp Ile Asp Phe 
385                 390                 395                 400 


Arg Tyr Pro Ser Ile Thr Arg Asp Met Asp Tyr Val Glu Trp Leu Ala 
                405                 410                 415     


Asp Thr Met Ile Arg Val Pro Val Glu His Thr Leu Asp Ala Val Asn 
            420                 425                 430         


Ile Ala Asp Arg Tyr Asp Ala Lys Ala Val Lys Glu Arg Leu Ala Met 
        435                 440                 445             


Met Thr Pro Gln Asn Ala Arg Ile Trp Tyr Ile Ser Pro Lys Glu Pro 
    450                 455                 460                 


His Asn Lys Thr Ala Tyr Phe Val Asp Ala Pro Tyr Gln Val Asp Lys 
465                 470                 475                 480 


Ile Ser Ala Gln Thr Phe Ala Asp Trp Gln Lys Lys Ala Ala Asp Ile 
                485                 490                 495     


Ala Leu Ser Leu Pro Glu Leu Asn Pro Tyr Ile Pro Asp Asp Phe Ser 
            500                 505                 510         


Leu Ile Lys Ser Glu Lys Lys Tyr Asp His Pro Glu Leu Ile Val Asp 
        515                 520                 525             


Glu Ser Asn Leu Arg Val Val Tyr Ala Pro Ser Arg Tyr Phe Ala Ser 
    530                 535                 540                 


Glu Pro Lys Ala Asp Val Ser Leu Ile Leu Arg Asn Pro Lys Ala Met 
545                 550                 555                 560 


Asp Ser Ala Arg Asn Gln Val Met Phe Ala Leu Asn Asp Tyr Leu Ala 
                565                 570                 575     


Gly Leu Ala Leu Asp Gln Leu Ser Asn Gln Ala Ser Val Gly Gly Ile 
            580                 585                 590         


Ser Phe Ser Thr Asn Ala Asn Asn Gly Leu Met Val Asn Ala Asn Gly 
        595                 600                 605             


Tyr Thr Gln Arg Leu Pro Gln Leu Phe Gln Ala Leu Leu Glu Gly Tyr 
    610                 615                 620                 


Phe Ser Tyr Thr Ala Thr Glu Asp Gln Leu Glu Gln Ala Lys Ser Trp 
625                 630                 635                 640 


Tyr Asn Gln Met Met Asp Ser Ala Glu Lys Gly Lys Ala Phe Glu Gln 
                645                 650                 655     


Ala Ile Met Pro Ala Gln Met Leu Ser Gln Val Pro Tyr Phe Ser Arg 
            660                 665                 670         


Asp Glu Arg Arg Lys Ile Leu Pro Ser Ile Thr Leu Lys Glu Val Leu 
        675                 680                 685             


Ala Tyr Arg Asp Ala Leu Lys Ser Gly Ala Arg Pro Glu Phe Met Val 
    690                 695                 700                 


Ile Gly Asn Met Thr Glu Ala Gln Ala Thr Thr Leu Ala Arg Asp Val 
705                 710                 715                 720 


Gln Lys Gln Leu Gly Ala Asp Gly Ser Glu Trp Cys Arg Asn Lys Asp 
                725                 730                 735     


Val Val Val Asp Lys Lys Gln Ser Val Ile Phe Glu Lys Ala Gly Asn 
            740                 745                 750         


Ser Thr Asp Ser Ala Leu Ala Ala Val Phe Val Pro Thr Gly Tyr Asp 
        755                 760                 765             


Glu Tyr Thr Ser Ser Ala Tyr Ser Ser Leu Leu Gly Gln Ile Val Gln 
    770                 775                 780                 


Pro Trp Phe Tyr Asn Gln Leu Arg Thr Glu Glu Gln Leu Gly Tyr Ala 
785                 790                 795                 800 


Val Phe Ala Phe Pro Met Ser Val Gly Arg Gln Trp Gly Met Gly Phe 
                805                 810                 815     


Leu Leu Gln Ser Asn Asp Lys Gln Pro Ser Phe Leu Trp Glu Arg Tyr 
            820                 825                 830         


Lys Ala Phe Phe Pro Thr Ala Glu Ala Lys Leu Arg Ala Met Lys Pro 
        835                 840                 845             


Asp Glu Phe Ala Gln Ile Gln Gln Ala Val Ile Thr Gln Met Leu Gln 
    850                 855                 860                 


Ala Pro Gln Thr Leu Gly Glu Glu Ala Ser Lys Leu Ser Lys Asp Phe 
865                 870                 875                 880 


Asp Arg Gly Asn Met Arg Phe Asp Ser Arg Asp Lys Ile Val Ala Gln 
                885                 890                 895     


Ile Lys Leu Leu Thr Pro Gln Lys Leu Ala Asp Phe Phe His Gln Ala 
            900                 905                 910         


Val Val Glu Pro Gln Gly Met Ala Ile Leu Ser Gln Ile Ser Gly Ser 
        915                 920                 925             


Gln Asn Gly Lys Ala Glu Tyr Val His Pro Glu Gly Trp Lys Val Trp 
    930                 935                 940                 


Glu Asn Val Ser Ala Leu Gln Gln Thr Met Pro Leu Met Ser Glu Lys 
945                 950                 955                 960 


Asn Glu 
        


<210>  6
<211>  2915
<212>  DNA
<213>  E. coli

<400>  6
attccccgca gcacctggtt caaagcatta ttgttgttag ttgccctttg ggcacattaa       60

tgtcaggcag aaacgggatg gcagccgatt caggaaacca tccgtaaaag tgataaagat      120

aaccgccagt atcaggctat acgtctggat aacggtatgg tggtcttgct ggtttctgat      180

ccgcaggcag ttaaatcgct ctcggcgctg gtggtgcccg ttgggtcgct ggaagatccc      240

gaggcgtacc aggggctggc acattacctt gaacatatga gtctgatggg gtcgaaaaag      300

tacccgcagg ctgacagtct ggccgaatat ctcaaaatgc acggcggtag tcacaatgcc      360

agcactgcgc cgtatcgcac ggctttctat ctggaagttg agaacgacgc cttgcctggt      420

gcggtagacc gcctggccga tgctattgct gaacctttgc tcgacaagaa atatgccgaa      480

cgtgagcgta atgcggtgaa cgctgaatta accatggcgc gtacgcgtga cgggatgcgc      540

atggcacagg tcagcgcaga aaccattaac ccggcacacc ccggttcaaa gttttctggt      600

ggtaacctcg aaactttaag cgacaaacct ggtaatccgg tgcagcaggc gctgaaagat      660

ttccacgaga agtactattc cgccaatttg atgaaggcgg ttatttacag taataaaccg      720

ctgccggagt tggcaaaaat ggcggcggac acctttggtc gcgtgccgaa caaagagagc      780

aaaaaaccgg aaatcaccgt gccggtagtc accgacgcgc aaaagggcat tatcattcat      840

tacgtccctg cgctgccgcg taaagtgttg cgcgttgagt ttcgcatcga taacaactca      900

gcgaagttcc gtagtaaaac cgatgaattg attacctatc tgattggcaa tcgcagccca      960

ggtacacttt ctgactggct gcaaaagcag ggattagttg agggcattag cgccaactcc     1020

gatcctatcg tcaacggcaa cagcggcgta ttagcgatct ctgcgtcttt aaccgataaa     1080

ggcctggcta atcgcgatca ggttgtggcg gcaattttta gctatctcaa tctgttacgt     1140

gaaaaaggca ttgataaaca atacttcgat gaactggcga atgtgctgga tatcgacttc     1200

cgttatccgt cgatcacccg tgatatggat tacgtcgaat ggctggcaga taccatgatt     1260

cgcgttcctg ttgagcatac gctggatgca gtcaatattg ccgatcggta cgatgctaaa     1320

gcagtaaagg aacgtctggc gatgatgacg ccgcagaatg cgcgtatctg gtatatcagc     1380

ccgaaagagc cgcacaacaa aacggcttac tttgtcgatg cgccgtatca ggtcgataaa     1440

atcagcgcac aaactttcgc cgactggcag aaaaaagccg ccgacattgc gctctctttg     1500

ccagagctta acccttatat tcctgatgat ttctcgctga ttaagtcaga gaagaaatac     1560

gaccatccag agctgattgt tgatgagtcg aatctgcgcg tggtgtatgc gccaagccgt     1620

tattttgcca gcgagcccaa agctgatgtc agcctgattt tgcgtaatcc gaaagccatg     1680

gacagcgccc gcaatcaggt gatgtttgcg ctcaatgatt atctcgcagg gctggcgctt     1740

gatcagttaa gcaaccaggc gtcggttggt ggcataagtt tttccaccaa cgctaacaac     1800

ggccttatgg ttaatgctaa tggttacacc cagcgtctgc cgcagctgtt ccaggcattg     1860

ctcgaggggt actttagcta taccgctacg gaagatcagc ttgagcaggc gaagtcctgg     1920

tataaccaga tgatggattc cgcagaaaag ggtaaagcgt ttgagcaggc gattatgccc     1980

gcgcagatgc tctcgcaagt gccgtacttc tcgcgagatg aacggcgtaa aattttgccc     2040

tccattacgt tgaaagaggt gctggcctat cgcgacgcct taaaatcagg ggctcgacca     2100

gagtttatgg ttatcggcaa catgaccgag gcccaggcaa caacgctggc acgcgatgtg     2160

caaaaacagt tgggcgctga tggttcagag tggtgtcgaa acaaagatgt agtggtcgat     2220

aaaaaacaat ccgtcatctt tgaaaaagcc ggtaacagca ccgactccgc actggcagcg     2280

gtatttgtac cgactggcta cgatgaatac accagctcag cctatagctc tctgttgggg     2340

cagatcgtac agccgtggtt ctacaatcag ttgcgtaccg aagaacaatt gggctatgcc     2400

gtgtttgcgt ttccaatgag cgtggggcgt cagtggggca tgggcttcct tttgcaaagc     2460

aatgataaac agccttcatt cttgtgggag cgttacaagg cgtttttccc aaccgcagag     2520

gcaaaattgc gagcgatgaa gccagatgag tttgcgcaaa tccagcaggc ggtaattacc     2580

cagatgctgc aggcaccgca aacgctcggc gaagaagcat cgaagttaag taaagatttc     2640

gatcgcggca atatgcgctt cgattcgcgt gataaaatcg tggcccagat aaaactgctg     2700

acgccgcaaa aacttgctga tttcttccat caggcggtgg tcgagccgca aggcatggct     2760

attctgtcgc agatttccgg cagccagaac gggaaagccg aatatgtaca ccctgaaggc     2820

tggaaagtgt gggagaacgt cagcgcgttg cagcaaacaa tgcccctgat gagtgaaaag     2880

aatgagtgat gtcgccgaga cactagatcc tttgc                                2915


<210>  7
<211>  1425
<212>  DNA
<213>  E. coli

<400>  7
atgaaaaaaa ccacattagc actgagtgca ctggctctga gtttaggttt ggcgttatct       60

ccgctctctg caacggcggc tgagacttct tcagcaacga cagcccagca gatgccaagc      120

cttgcaccga tgctcgaaaa ggtgatgcct tcagtggtca gcattaacgt agaaggtagc      180

acaaccgtta atacgccgcg tatgccgcgt aatttccagc agttcttcgg tgatgattct      240

ccgttctgcc aggaaggttc tccgttccag agctctccgt tctgccaggg tggccagggc      300

ggtaatggtg gcggccagca acagaaattc atggcgctgg gttccggcgt catcattgat      360

gccgataaag gctatgtcgt caccaacaac cacgttgttg ataacgcgac ggtcattaaa      420

gttcaactga gcgatggccg taagttcgac gcgaagatgg ttggcaaaga tccgcgctct      480

gatatcgcgc tgatccaaat ccagaacccg aaaaacctga ccgcaattaa gatggcggat      540

tctgatgcac tgcgcgtggg tgattacacc gtagcgattg gtaacccgtt tggtctgggc      600

gagacggtaa cttccgggat tgtctctgcg ctggggcgta gcggcctgaa tgccgaaaac      660

tacgaaaact tcatccagac cgatgcagcg atcaaccgtg gtaactccgg tggtgcgctg      720

gttaacctga acggcgaact gatcggtatc aacaccgcga tcctcgcacc ggacggcggc      780

aacatcggta tcggttttgc tatcccgagt aacatggtga aaaacctgac ctcgcagatg      840

gtggaatacg gccaggtgaa acgcggtgag ctgggtatta tggggactga gctgaactcc      900

gaactggcga aagcgatgaa agttgacgcc cagcgcggtg ctttcgtaag ccaggttctg      960

cctaattcct ccgctgcaaa agcgggcatt aaagcgggtg atgtgatcac ctcactgaac     1020

ggtaagccga tcagcagctt tgccgcactg cgtgctcagg tgggtactat gccggtaggc     1080

agcaaactga ccctgggctt actgcgcgac ggtaagcagg ttaacgtgaa cctggaactg     1140

cagcagagca gccagaatca ggttgattcc agctccatct tcaacggcat tgaaggcgct     1200

gagatgagca acaaaggcaa agatcagggc gtggtagtga acaacgtgaa aacgggcact     1260

ccggctgcgc agatcggcct gaagaaaggt gatgtgatta ttggcgcgaa ccagcaggca     1320

gtgaaaaaca tcgctgaact gcgtaaagtt ctcgacagca aaccgtctgt gctggcactc     1380

aacattcagc gcggcgacag caccatctac ctgttaatgc agtaa                     1425


<210>  8
<211>  474
<212>  PRT
<213>  E. coli

<400>  8

Met Lys Lys Thr Thr Leu Ala Leu Ser Ala Leu Ala Leu Ser Leu Gly 
1               5                   10                  15      


Leu Ala Leu Ser Pro Leu Ser Ala Thr Ala Ala Glu Thr Ser Ser Ala 
            20                  25                  30          


Thr Thr Ala Gln Gln Met Pro Ser Leu Ala Pro Met Leu Glu Lys Val 
        35                  40                  45              


Met Pro Ser Val Val Ser Ile Asn Val Glu Gly Ser Thr Thr Val Asn 
    50                  55                  60                  


Thr Pro Arg Met Pro Arg Asn Phe Gln Gln Phe Phe Gly Asp Asp Ser 
65                  70                  75                  80  


Pro Phe Cys Gln Glu Gly Ser Pro Phe Gln Ser Ser Pro Phe Cys Gln 
                85                  90                  95      


Gly Gly Gln Gly Gly Asn Gly Gly Gly Gln Gln Gln Lys Phe Met Ala 
            100                 105                 110         


Leu Gly Ser Gly Val Ile Ile Asp Ala Asp Lys Gly Tyr Val Val Thr 
        115                 120                 125             


Asn Asn His Val Val Asp Asn Ala Thr Val Ile Lys Val Gln Leu Ser 
    130                 135                 140                 


Asp Gly Arg Lys Phe Asp Ala Lys Met Val Gly Lys Asp Pro Arg Ser 
145                 150                 155                 160 


Asp Ile Ala Leu Ile Gln Ile Gln Asn Pro Lys Asn Leu Thr Ala Ile 
                165                 170                 175     


Lys Met Ala Asp Ser Asp Ala Leu Arg Val Gly Asp Tyr Thr Val Ala 
            180                 185                 190         


Ile Gly Asn Pro Phe Gly Leu Gly Glu Thr Val Thr Ser Gly Ile Val 
        195                 200                 205             


Ser Ala Leu Gly Arg Ser Gly Leu Asn Ala Glu Asn Tyr Glu Asn Phe 
    210                 215                 220                 


Ile Gln Thr Asp Ala Ala Ile Asn Arg Gly Asn Ser Gly Gly Ala Leu 
225                 230                 235                 240 


Val Asn Leu Asn Gly Glu Leu Ile Gly Ile Asn Thr Ala Ile Leu Ala 
                245                 250                 255     


Pro Asp Gly Gly Asn Ile Gly Ile Gly Phe Ala Ile Pro Ser Asn Met 
            260                 265                 270         


Val Lys Asn Leu Thr Ser Gln Met Val Glu Tyr Gly Gln Val Lys Arg 
        275                 280                 285             


Gly Glu Leu Gly Ile Met Gly Thr Glu Leu Asn Ser Glu Leu Ala Lys 
    290                 295                 300                 


Ala Met Lys Val Asp Ala Gln Arg Gly Ala Phe Val Ser Gln Val Leu 
305                 310                 315                 320 


Pro Asn Ser Ser Ala Ala Lys Ala Gly Ile Lys Ala Gly Asp Val Ile 
                325                 330                 335     


Thr Ser Leu Asn Gly Lys Pro Ile Ser Ser Phe Ala Ala Leu Arg Ala 
            340                 345                 350         


Gln Val Gly Thr Met Pro Val Gly Ser Lys Leu Thr Leu Gly Leu Leu 
        355                 360                 365             


Arg Asp Gly Lys Gln Val Asn Val Asn Leu Glu Leu Gln Gln Ser Ser 
    370                 375                 380                 


Gln Asn Gln Val Asp Ser Ser Ser Ile Phe Asn Gly Ile Glu Gly Ala 
385                 390                 395                 400 


Glu Met Ser Asn Lys Gly Lys Asp Gln Gly Val Val Val Asn Asn Val 
                405                 410                 415     


Lys Thr Gly Thr Pro Ala Ala Gln Ile Gly Leu Lys Lys Gly Asp Val 
            420                 425                 430         


Ile Ile Gly Ala Asn Gln Gln Ala Val Lys Asn Ile Ala Glu Leu Arg 
        435                 440                 445             


Lys Val Leu Asp Ser Lys Pro Ser Val Leu Ala Leu Asn Ile Gln Arg 
    450                 455                 460                 


Gly Asp Ser Thr Ile Tyr Leu Leu Met Gln 
465                 470                 


<210>  9
<211>  1425
<212>  DNA
<213>  E. coli

<400>  9
atgaaaaaaa ccacattagc actgagtgca ctggctctga gtttaggttt ggcgttatct       60

ccgctctctg caacggcggc tgagacttct tcagcaacga cagcccagca gatgccaagc      120

cttgcaccga tgctcgaaaa ggtgatgcct tcagtggtca gcattaacgt agaaggtagc      180

acaaccgtta atacgccgcg tatgccgcgt aatttccagc agttcttcgg tgatgattct      240

ccgttctgcc aggaaggttc tccgttccag agctctccgt tctgccaggg tggccagggc      300

ggtaatggtg gcggccagca acagaaattc atggcgctgg gttccggcgt catcattgat      360

gccgataaag gctatgtcgt caccaacaac cacgttgttg ataacgcgac ggtcattaaa      420

gttcaactga gcgatggccg taagttcgac gcgaagatgg ttggcaaaga tccgcgctct      480

gatatcgcgc tgatccaaat ccagaacccg aaaaacctga ccgcaattaa gatggcggat      540

tctgatgcac tgcgcgtggg tgattacacc gtagcgattg gtaacccgtt tggtctgggc      600

gagacggtaa cttccgggat tgtctctgcg ctggggcgta gcggcctgaa tgccgaaaac      660

tacgaaaact tcatccagac cgatgcagcg attaatcgtg gtaacgccgg tggtgcgctg      720

gttaacctga acggcgaact gatcggtatc aacaccgcga tcctcgcacc ggacggcggc      780

aacatcggta tcggttttgc tatcccgagt aacatggtga aaaacctgac ctcgcagatg      840

gtggaatacg gccaggtgaa acgcggtgag ctgggtatta tggggactga gctgaactcc      900

gaactggcga aagcgatgaa agttgacgcc cagcgcggtg ctttcgtaag ccaggttctg      960

cctaattcct ccgctgcaaa agcgggcatt aaagcgggtg atgtgatcac ctcactgaac     1020

ggtaagccga tcagcagctt tgccgcactg cgtgctcagg tgggtactat gccggtaggc     1080

agcaaactga ccctgggctt actgcgcgac ggtaagcagg ttaacgtgaa cctggaactg     1140

cagcagagca gccagaatca ggttgattcc agctccatct tcaacggcat tgaaggcgct     1200

gagatgagca acaaaggcaa agatcagggc gtggtagtga acaacgtgaa aacgggcact     1260

ccggctgcgc agatcggcct gaagaaaggt gatgtgatta ttggcgcgaa ccagcaggca     1320

gtgaaaaaca tcgctgaact gcgtaaagtt ctcgacagca aaccgtctgt gctggcactc     1380

aacattcagc gcggcgacag caccatctac ctgttaatgc agtaa                     1425


<210>  10
<211>  474
<212>  PRT
<213>  E. coli

<400>  10

Met Lys Lys Thr Thr Leu Ala Leu Ser Ala Leu Ala Leu Ser Leu Gly 
1               5                   10                  15      


Leu Ala Leu Ser Pro Leu Ser Ala Thr Ala Ala Glu Thr Ser Ser Ala 
            20                  25                  30          


Thr Thr Ala Gln Gln Met Pro Ser Leu Ala Pro Met Leu Glu Lys Val 
        35                  40                  45              


Met Pro Ser Val Val Ser Ile Asn Val Glu Gly Ser Thr Thr Val Asn 
    50                  55                  60                  


Thr Pro Arg Met Pro Arg Asn Phe Gln Gln Phe Phe Gly Asp Asp Ser 
65                  70                  75                  80  


Pro Phe Cys Gln Glu Gly Ser Pro Phe Gln Ser Ser Pro Phe Cys Gln 
                85                  90                  95      


Gly Gly Gln Gly Gly Asn Gly Gly Gly Gln Gln Gln Lys Phe Met Ala 
            100                 105                 110         


Leu Gly Ser Gly Val Ile Ile Asp Ala Asp Lys Gly Tyr Val Val Thr 
        115                 120                 125             


Asn Asn His Val Val Asp Asn Ala Thr Val Ile Lys Val Gln Leu Ser 
    130                 135                 140                 


Asp Gly Arg Lys Phe Asp Ala Lys Met Val Gly Lys Asp Pro Arg Ser 
145                 150                 155                 160 


Asp Ile Ala Leu Ile Gln Ile Gln Asn Pro Lys Asn Leu Thr Ala Ile 
                165                 170                 175     


Lys Met Ala Asp Ser Asp Ala Leu Arg Val Gly Asp Tyr Thr Val Ala 
            180                 185                 190         


Ile Gly Asn Pro Phe Gly Leu Gly Glu Thr Val Thr Ser Gly Ile Val 
        195                 200                 205             


Ser Ala Leu Gly Arg Ser Gly Leu Asn Ala Glu Asn Tyr Glu Asn Phe 
    210                 215                 220                 


Ile Gln Thr Asp Ala Ala Ile Asn Arg Gly Asn Ala Gly Gly Ala Leu 
225                 230                 235                 240 


Val Asn Leu Asn Gly Glu Leu Ile Gly Ile Asn Thr Ala Ile Leu Ala 
                245                 250                 255     


Pro Asp Gly Gly Asn Ile Gly Ile Gly Phe Ala Ile Pro Ser Asn Met 
            260                 265                 270         


Val Lys Asn Leu Thr Ser Gln Met Val Glu Tyr Gly Gln Val Lys Arg 
        275                 280                 285             


Gly Glu Leu Gly Ile Met Gly Thr Glu Leu Asn Ser Glu Leu Ala Lys 
    290                 295                 300                 


Ala Met Lys Val Asp Ala Gln Arg Gly Ala Phe Val Ser Gln Val Leu 
305                 310                 315                 320 


Pro Asn Ser Ser Ala Ala Lys Ala Gly Ile Lys Ala Gly Asp Val Ile 
                325                 330                 335     


Thr Ser Leu Asn Gly Lys Pro Ile Ser Ser Phe Ala Ala Leu Arg Ala 
            340                 345                 350         


Gln Val Gly Thr Met Pro Val Gly Ser Lys Leu Thr Leu Gly Leu Leu 
        355                 360                 365             


Arg Asp Gly Lys Gln Val Asn Val Asn Leu Glu Leu Gln Gln Ser Ser 
    370                 375                 380                 


Gln Asn Gln Val Asp Ser Ser Ser Ile Phe Asn Gly Ile Glu Gly Ala 
385                 390                 395                 400 


Glu Met Ser Asn Lys Gly Lys Asp Gln Gly Val Val Val Asn Asn Val 
                405                 410                 415     


Lys Thr Gly Thr Pro Ala Ala Gln Ile Gly Leu Lys Lys Gly Asp Val 
            420                 425                 430         


Ile Ile Gly Ala Asn Gln Gln Ala Val Lys Asn Ile Ala Glu Leu Arg 
        435                 440                 445             


Lys Val Leu Asp Ser Lys Pro Ser Val Leu Ala Leu Asn Ile Gln Arg 
    450                 455                 460                 


Gly Asp Ser Thr Ile Tyr Leu Leu Met Gln 
465                 470                 


<210>  11
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hTNF40-gL1

<400>  11

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Val Gly Thr Asn 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Ala Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Tyr Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Asn Ile Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  12
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gh3h TNF40.4

<400>  12

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Val Phe Thr Asp Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Ile Gly Glu Pro Ile Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Phe Ser Leu Asp Thr Ser Lys Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Tyr Arg Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  13
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Grafted Light Chain

<400>  13

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Val Gly Thr Asn 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Ala Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Tyr Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Asn Ile Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  14
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Grafted Heavy Chain

<400>  14

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Val Phe Thr Asp Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Ile Gly Glu Pro Ile Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Phe Ser Leu Asp Thr Ser Lys Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Tyr Arg Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr 
    210                 215                 220                 


His Thr Cys Ala Ala 
225                 


<210>  15
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  15
gcatcataat tttcttttta cctc                                              24


<210>  16
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  16
gggaaatgaa cctgagcaaa acgc                                              24


<210>  17
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  17
gtgccaggag atgcagcagc ttgc                                              24


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  18
tttgcagcca gtcagaaagt g                                                 21


<210>  19
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  19
ctgcctgcga ttttcgccgg aacg                                              24


<210>  20
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Oligonucleotide primer

<400>  20
cgcatggtac gtgccacgat atcc                                              24


<210>  21
<211>  188
<212>  PRT
<213>  Escherichia coli

<400>  21

Met Val Lys Ser Gln Pro Ile Leu Arg Tyr Ile Leu Arg Gly Ile Pro 
1               5                   10                  15      


Ala Ile Ala Val Ala Val Leu Leu Ser Ala Cys Ser Ala Asn Asn Thr 
            20                  25                  30          


Ala Lys Asn Met His Pro Glu Thr Arg Ala Val Gly Ser Glu Thr Ser 
        35                  40                  45              


Ser Leu Gln Ala Ser Gln Asp Glu Phe Glu Asn Leu Val Arg Asn Val 
    50                  55                  60                  


Asp Val Lys Ser Arg Ile Met Asp Gln Tyr Ala Asp Trp Lys Gly Val 
65                  70                  75                  80  


Arg Tyr Arg Leu Gly Gly Ser Thr Lys Lys Gly Ile Asp Cys Ser Gly 
                85                  90                  95      


Phe Val Gln Arg Thr Phe Arg Glu Gln Phe Gly Leu Glu Leu Pro Arg 
            100                 105                 110         


Ser Thr Tyr Glu Gln Gln Glu Met Gly Lys Ser Val Ser Arg Ser Asn 
        115                 120                 125             


Leu Arg Thr Gly Asp Leu Val Leu Phe Arg Ala Gly Ser Thr Gly Arg 
    130                 135                 140                 


His Val Gly Ile Tyr Ile Gly Asn Asn Gln Phe Val His Ala Ser Thr 
145                 150                 155                 160 


Ser Ser Gly Val Ile Ile Ser Ser Met Asn Glu Pro Tyr Trp Lys Lys 
                165                 170                 175     


Arg Tyr Asn Glu Ala Arg Arg Val Leu Ser Arg Ser 
            180                 185             


<210>  22
<211>  162
<212>  PRT
<213>  Escherichia coli

<400>  22

Cys Ser Ala Asn Asn Thr Ala Lys Asn Met His Pro Glu Thr Arg Ala 
1               5                   10                  15      


Val Gly Ser Glu Thr Ser Ser Leu Gln Ala Ser Gln Asp Glu Phe Glu 
            20                  25                  30          


Asn Leu Val Arg Asn Val Asp Val Lys Ser Arg Ile Met Asp Gln Tyr 
        35                  40                  45              


Ala Asp Trp Lys Gly Val Arg Tyr Arg Leu Gly Gly Ser Thr Lys Lys 
    50                  55                  60                  


Gly Ile Asp Cys Ser Gly Phe Val Gln Arg Thr Phe Arg Glu Gln Phe 
65                  70                  75                  80  


Gly Leu Glu Leu Pro Arg Ser Thr Tyr Glu Gln Gln Glu Met Gly Lys 
                85                  90                  95      


Ser Val Ser Arg Ser Asn Leu Arg Thr Gly Asp Leu Val Leu Phe Arg 
            100                 105                 110         


Ala Gly Ser Thr Gly Arg His Val Gly Ile Tyr Ile Gly Asn Asn Gln 
        115                 120                 125             


Phe Val His Ala Ser Thr Ser Ser Gly Val Ile Ile Ser Ser Met Asn 
    130                 135                 140                 


Glu Pro Tyr Trp Lys Lys Arg Tyr Asn Glu Ala Arg Arg Val Leu Ser 
145                 150                 155                 160 


Arg Ser 
        


<210>  23
<211>  951
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutated OmpT sequence

<400>  23
atgcgggcga aacttctggg aatagtcctg acaaccccta ttgcgatcag ctcttttgct       60

tctaccgaga ctttatcgtt tactcctgac aacataaatg cggacattag tcttggaact      120

ctgagcggaa aaacaaaaga gcgtgtttat ctagccgaag aaggaggccg aaaagtcagt      180

caactcgact ggaaattcaa taacgctgca attattaaag gtgcaattaa ttgggatttg      240

atgccccaga tatctatcgg ggctgctggc tggacaactc tcggcagccg aggtggcaat      300

atggtcgatc aggactggat ggattccagt aaccccggaa cctggacgga tgaaagtaga      360

caccctgata cacaactcaa ttatgccaac gaatttgatc tgaatatcaa aggctggctc      420

ctcaacgaac ccaattaccg cctgggactc atggccggat atcaggaaag ccgttatagc      480

tttacagcca gaggtggttc ctatatctac agttctgagg agggattcag agatgatatc      540

ggctccttcc cgaatggaga aagagcaatc ggctacaaac aacgttttaa aatgccctac      600

attggcttga ctggaagtta tcgttatgaa gattttgaac tcggtggcac atttaaatac      660

agcggctggg tggaatcatc tgataacgct gaagcttatg acccgggaaa aagaatcact      720

tatcgcagta aggtcaaaga ccaaaattac tattctgttg cagtcaatgc aggttattac      780

gtcacaccta acgcaaaagt ttatgttgaa ggcgcatgga atcgggttac gaataaaaaa      840

ggtaatactt cactttatga tcacaataat aacacttcag actacagcaa aaatggagca      900

ggtatagaaa actataactt catcactact gctggtctta agtacacatt t               951


<210>  24
<211>  317
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated OmpT sequence

<400>  24

Met Arg Ala Lys Leu Leu Gly Ile Val Leu Thr Thr Pro Ile Ala Ile 
1               5                   10                  15      


Ser Ser Phe Ala Ser Thr Glu Thr Leu Ser Phe Thr Pro Asp Asn Ile 
            20                  25                  30          


Asn Ala Asp Ile Ser Leu Gly Thr Leu Ser Gly Lys Thr Lys Glu Arg 
        35                  40                  45              


Val Tyr Leu Ala Glu Glu Gly Gly Arg Lys Val Ser Gln Leu Asp Trp 
    50                  55                  60                  


Lys Phe Asn Asn Ala Ala Ile Ile Lys Gly Ala Ile Asn Trp Asp Leu 
65                  70                  75                  80  


Met Pro Gln Ile Ser Ile Gly Ala Ala Gly Trp Thr Thr Leu Gly Ser 
                85                  90                  95      


Arg Gly Gly Asn Met Val Asp Gln Asp Trp Met Asp Ser Ser Asn Pro 
            100                 105                 110         


Gly Thr Trp Thr Asp Glu Ser Arg His Pro Asp Thr Gln Leu Asn Tyr 
        115                 120                 125             


Ala Asn Glu Phe Asp Leu Asn Ile Lys Gly Trp Leu Leu Asn Glu Pro 
    130                 135                 140                 


Asn Tyr Arg Leu Gly Leu Met Ala Gly Tyr Gln Glu Ser Arg Tyr Ser 
145                 150                 155                 160 


Phe Thr Ala Arg Gly Gly Ser Tyr Ile Tyr Ser Ser Glu Glu Gly Phe 
                165                 170                 175     


Arg Asp Asp Ile Gly Ser Phe Pro Asn Gly Glu Arg Ala Ile Gly Tyr 
            180                 185                 190         


Lys Gln Arg Phe Lys Met Pro Tyr Ile Gly Leu Thr Gly Ser Tyr Arg 
        195                 200                 205             


Tyr Glu Asp Phe Glu Leu Gly Gly Thr Phe Lys Tyr Ser Gly Trp Val 
    210                 215                 220                 


Glu Ser Ser Asp Asn Ala Glu Ala Tyr Asp Pro Gly Lys Arg Ile Thr 
225                 230                 235                 240 


Tyr Arg Ser Lys Val Lys Asp Gln Asn Tyr Tyr Ser Val Ala Val Asn 
                245                 250                 255     


Ala Gly Tyr Tyr Val Thr Pro Asn Ala Lys Val Tyr Val Glu Gly Ala 
            260                 265                 270         


Trp Asn Arg Val Thr Asn Lys Lys Gly Asn Thr Ser Leu Tyr Asp His 
        275                 280                 285             


Asn Asn Asn Thr Ser Asp Tyr Ser Lys Asn Gly Ala Gly Ile Glu Asn 
    290                 295                 300                 


Tyr Asn Phe Ile Thr Thr Ala Gly Leu Lys Tyr Thr Phe 
305                 310                 315         


<210>  25
<211>  954
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutated OmpT sequence

<400>  25
attcgggcga aacttctggg aatagtcctg acaaccccta ttgcgatcag ctcttttgct       60

tctaccgaga ctttatcgtt tactcctgac aacataaatg cggacattag tcttggaact      120

ctgagcggaa aaacaaaaga gcgtgtttat ctagccgaag aaggaggccg aaaagtcagt      180

caactcgact ggaaattcaa taacgctgca attattaaag gtgcaattaa ttgggatttg      240

atgccccaga tatctatcgg ggctgctggc tggacaactc tcggcagccg aggtggcaat      300

atggtcgatc aggactggat ggattccagt aaccccggaa cctggacgga tgaaagtaga      360

caccctgata cacaactcaa ttatgccaac gaatttgatc tgaatatcaa aggctggctc      420

ctcaacgaac ccaattaccg cctgggactc atggccggat atcaggaaag ccgttatagc      480

tttacagcca gaggtggttc ctatatctac agttctgagg agggattcag agatgatatc      540

ggctccttcc cgaatggaga aagagcaatc ggctacaaac aacgttttaa aatgccctac      600

attggcttga ctggaagtta tcgttatgaa gattttgaac tcggtggcac atttaaatac      660

agcggctggg tggaatcatc tgataacgat gaacactatg acccgggaaa aagaatcact      720

tatcgcagta aggtcaaaga ccaaaattac tattctgttg cagtcaatgc aggttattac      780

gtcacaccta acgcaaaagt ttatgttgaa ggcgcatgga atcgggttac gaataaaaaa      840

ggtaatactt cactttatga tcacaataat aacacttcag actacagcaa aaatggagca      900

ggtatagaaa actataactt catcactact gctggtctta agtacacatt ttaa            954


<210>  26
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence:hTNF40 CDRH1

<400>  26

Asp Tyr Gly Met Asn 
1               5   


<210>  27
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence:hTNF40 Human hybrid CDRH2

<400>  27

Trp Ile Asn Thr Tyr Ile Gly Glu Pro Ile Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  28
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence:hTNF40 CDRH3

<400>  28

Gly Tyr Arg Ser Tyr Ala Met Asp Tyr 
1               5                   


<210>  29
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence:hTNF40 CDRL1

<400>  29

Lys Ala Ser Gln Asn Val Gly Thr Asn Val Ala 
1               5                   10      


<210>  30
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence:hTNF40 CDRL2

<400>  30

Ser Ala Ser Phe Leu Tyr Ser 
1               5           


<210>  31
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence:hTNF40 CDRL3

<400>  31

Gln Gln Tyr Asn Ile Tyr Pro Leu Thr 
1               5                   


<210>  32
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence:hTNF40 CDRH2

<400>  32

Trp Ile Asn Thr Tyr Ile Gly Glu Pro Ile Tyr Val Asp Asp Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  33
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OmpA oligonucleotide adaptor

<400>  33
tcgagttcta gataacgagg cgtaaaaaat gaaaaagaca gctatcgcaa ttgcagtggc       60

cttggctctg acgtacgagt cagg                                              84


<210>  34
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IGS cassette-1

<400>  34
gagctcacca gtaacaaaaa gttttaatag aggagagtgt taatgaagaa gactgctata       60

gcaattg                                                                 67


<210>  35
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IGS cassette-2

<400>  35
gagctcacca gtaacaaaaa gttttaatag aggggagtgt taaaatgaag aagactgcta       60

tagcaattg                                                               69


<210>  36
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IGS cassette-3

<400>  36
gagctcacca gtaacaaaaa gctttaatag aggagagtgt tgaggaggaa aaaaaaatga       60

agaaaactgc tatagcaatt g                                                 81


<210>  37
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IGS cassette-4

<400>  37
gagctcacca gtaacaaaaa gttttaatag aggagagtgt tgacgaggat tatataatga       60

agaaaactgc tatagcaatt g                                                 81


