                         SEQUENCE LISTING

<110>  Dow AGROSCIENCES LLC
 
<120>  COMBINED USE OF CRY1CA AND CRY1AB FOR MANAGEMENT OF RESISTANT INSECTS

<130>  DAS-P0162-US-02

<160>  3    

<170>  PatentIn version 3.5



<210>  1
<211>  1164
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DIG-152 Chimeric protein

<400>  1

Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu 
1               5                   10                  15      


Ser Asn Pro Glu Glu Val Leu Leu Asp Gly Glu Arg Ile Ser Thr Gly 
            20                  25                  30          


Asn Ser Ser Ile Asp Ile Ser Leu Ser Leu Val Gln Phe Leu Val Ser 
        35                  40                  45              


Asn Phe Val Pro Gly Gly Gly Phe Leu Val Gly Leu Ile Asp Phe Val 
    50                  55                  60                  


Trp Gly Ile Val Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile 
65                  70                  75                  80  


Glu Gln Leu Ile Asn Glu Arg Ile Ala Glu Phe Ala Arg Asn Ala Ala 
                85                  90                  95      


Ile Ala Asn Leu Glu Gly Leu Gly Asn Asn Phe Asn Ile Tyr Val Glu 
            100                 105                 110         


Ala Phe Lys Glu Trp Glu Glu Asp Pro Lys Asn Pro Ala Thr Arg Thr 
        115                 120                 125             


Arg Val Ile Asp Arg Phe Arg Ile Leu Asp Gly Leu Leu Glu Arg Asp 
    130                 135                 140                 


Ile Pro Ser Phe Arg Ile Ser Gly Phe Glu Val Pro Leu Leu Ser Val 
145                 150                 155                 160 


Tyr Ala Gln Ala Ala Asn Leu His Leu Ala Ile Leu Arg Asp Ser Val 
                165                 170                 175     


Ile Phe Gly Glu Arg Trp Gly Leu Thr Thr Ile Asn Val Asn Glu Asn 
            180                 185                 190         


Tyr Asn Arg Leu Ile Arg His Ile Asp Glu Tyr Ala Asp His Cys Ala 
        195                 200                 205             


Asn Thr Tyr Asn Arg Gly Leu Asn Asn Leu Pro Lys Ser Thr Tyr Gln 
    210                 215                 220                 


Asp Trp Ile Thr Tyr Asn Arg Leu Arg Arg Asp Leu Thr Leu Thr Val 
225                 230                 235                 240 


Leu Asp Ile Ala Ala Phe Phe Pro Asn Tyr Asp Asn Arg Arg Tyr Pro 
                245                 250                 255     


Ile Gln Pro Val Gly Gln Leu Thr Arg Glu Val Tyr Thr Asp Pro Leu 
            260                 265                 270         


Ile Asn Phe Asn Pro Gln Leu Gln Ser Val Ala Gln Leu Pro Thr Phe 
        275                 280                 285             


Asn Val Met Glu Asn Ser Ala Ile Arg Asn Pro His Leu Phe Asp Ile 
    290                 295                 300                 


Leu Asn Asn Leu Thr Ile Phe Thr Asp Trp Phe Ser Val Gly Arg Asn 
305                 310                 315                 320 


Phe Tyr Trp Gly Gly His Arg Val Ile Ser Ser Leu Ile Gly Gly Gly 
                325                 330                 335     


Asn Ile Thr Ser Pro Ile Tyr Gly Arg Glu Ala Asn Gln Glu Pro Pro 
            340                 345                 350         


Arg Ser Phe Thr Phe Asn Gly Pro Val Phe Arg Thr Leu Ser Asn Pro 
        355                 360                 365             


Thr Leu Arg Leu Leu Gln Gln Pro Trp Pro Ala Pro Pro Phe Asn Leu 
    370                 375                 380                 


Arg Gly Val Glu Gly Val Glu Phe Ser Thr Pro Thr Asn Ser Phe Thr 
385                 390                 395                 400 


Tyr Arg Gly Arg Gly Thr Val Asp Ser Leu Thr Glu Leu Pro Pro Glu 
                405                 410                 415     


Asp Asn Ser Val Pro Pro Arg Glu Gly Tyr Ser His Arg Leu Cys His 
            420                 425                 430         


Ala Thr Phe Val Gln Arg Ser Gly Thr Pro Phe Leu Thr Thr Gly Val 
        435                 440                 445             


Val Phe Ser Trp Thr His Arg Ser Ala Thr Leu Thr Asn Thr Ile Asp 
    450                 455                 460                 


Pro Glu Arg Ile Asn Gln Ile Pro Leu Val Lys Gly Phe Arg Val Trp 
465                 470                 475                 480 


Gly Gly Thr Ser Val Ile Thr Gly Pro Gly Phe Thr Gly Gly Asp Ile 
                485                 490                 495     


Leu Arg Arg Asn Thr Phe Gly Asp Phe Val Ser Leu Gln Val Asn Ile 
            500                 505                 510         


Asn Ser Pro Ile Thr Gln Arg Tyr Arg Leu Arg Phe Arg Tyr Ala Ser 
        515                 520                 525             


Ser Arg Asp Ala Arg Val Ile Val Leu Thr Gly Ala Ala Ser Thr Gly 
    530                 535                 540                 


Val Gly Gly Gln Val Ser Val Asn Met Pro Leu Gln Lys Thr Met Glu 
545                 550                 555                 560 


Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg Tyr Thr Asp Phe Ser 
                565                 570                 575     


Asn Pro Phe Ser Phe Arg Ala Asn Pro Asp Ile Ile Gly Ile Ser Glu 
            580                 585                 590         


Gln Pro Leu Phe Gly Ala Gly Ser Ile Ser Ser Gly Glu Leu Tyr Ile 
        595                 600                 605             


Asp Lys Ile Glu Ile Ile Leu Ala Asp Ala Thr Phe Glu Ala Glu Ser 
    610                 615                 620                 


Asp Leu Glu Arg Ala Gln Lys Ala Val Asn Ala Leu Phe Thr Ser Ser 
625                 630                 635                 640 


Asn Gln Ile Gly Leu Lys Thr Asp Val Thr Asp Tyr His Ile Asp Arg 
                645                 650                 655     


Val Ser Asn Leu Val Glu Cys Leu Ser Asp Glu Phe Cys Leu Asp Glu 
            660                 665                 670         


Lys Lys Glu Leu Ser Glu Lys Val Lys His Ala Lys Arg Leu Ser Asp 
        675                 680                 685             


Glu Arg Asn Leu Leu Gln Asp Pro Asn Phe Arg Gly Ile Asn Arg Gln 
    690                 695                 700                 


Leu Asp Arg Gly Trp Arg Gly Ser Thr Asp Ile Thr Ile Gln Gly Gly 
705                 710                 715                 720 


Asp Asp Val Phe Lys Glu Asn Tyr Val Thr Leu Leu Gly Thr Phe Asp 
                725                 730                 735     


Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln Lys Ile Asp Glu Ser Lys Leu 
            740                 745                 750         


Lys Ala Tyr Thr Arg Tyr Gln Leu Arg Gly Tyr Ile Glu Asp Ser Gln 
        755                 760                 765             


Asp Leu Glu Ile Tyr Leu Ile Arg Tyr Asn Ala Lys His Glu Thr Val 
    770                 775                 780                 


Asn Val Pro Gly Thr Gly Ser Leu Trp Pro Leu Ser Ala Pro Ser Pro 
785                 790                 795                 800 


Ile Gly Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile Asp 
                805                 810                 815     


Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile Phe 
            820                 825                 830         


Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu Phe 
        835                 840                 845             


Leu Glu Glu Lys Pro Leu Val Gly Glu Ala Leu Ala Arg Val Lys Arg 
    850                 855                 860                 


Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Lys Leu Glu Trp Glu Thr 
865                 870                 875                 880 


Asn Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe Val 
                885                 890                 895     


Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala Met Ile 
            900                 905                 910         


His Ala Ala Asp Lys Arg Val His Ser Ile Arg Glu Ala Tyr Leu Pro 
        915                 920                 925             


Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu Leu 
    930                 935                 940                 


Glu Gly Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn Val 
945                 950                 955                 960 


Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Ser Cys Trp Asn Val Lys 
                965                 970                 975     


Gly His Val Asp Val Glu Glu Gln Asn Asn His Arg Ser Val Leu Val 
            980                 985                 990         


Val Pro Glu Trp Glu Ala Glu Val  Ser Gln Glu Val Arg  Val Cys Pro 
        995                 1000                 1005             


Gly Arg  Gly Tyr Ile Leu Arg  Val Thr Ala Tyr Lys  Glu Gly Tyr 
    1010                 1015                 1020             


Gly Glu  Gly Cys Val Thr Ile  His Glu Ile Glu Asn  Asn Thr Asp 
    1025                 1030                 1035             


Glu Leu  Lys Phe Ser Asn Cys  Val Glu Glu Glu Val  Tyr Pro Asn 
    1040                 1045                 1050             


Asn Thr  Val Thr Cys Asn Asp  Tyr Thr Ala Thr Gln  Glu Glu Tyr 
    1055                 1060                 1065             


Glu Gly  Thr Tyr Thr Ser Arg  Asn Arg Gly Tyr Asp  Gly Ala Tyr 
    1070                 1075                 1080             


Glu Ser  Asn Ser Ser Val Pro  Ala Asp Tyr Ala Ser  Ala Tyr Glu 
    1085                 1090                 1095             


Glu Lys  Ala Tyr Thr Asp Gly  Arg Arg Asp Asn Pro  Cys Glu Ser 
    1100                 1105                 1110             


Asn Arg  Gly Tyr Gly Asp Tyr  Thr Pro Leu Pro Ala  Gly Tyr Val 
    1115                 1120                 1125             


Thr Lys  Glu Leu Glu Tyr Phe  Pro Glu Thr Asp Lys  Val Trp Ile 
    1130                 1135                 1140             


Glu Ile  Gly Glu Thr Glu Gly  Thr Phe Ile Val Asp  Ser Val Glu 
    1145                 1150                 1155             


Leu Leu  Leu Met Glu Glu 
    1160                 

 

<210>  2
<211>  619
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cry1Ca

<400>  2

Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu 
1               5                   10                  15      


Ser Asn Pro Glu Glu Val Leu Leu Asp Gly Glu Arg Ile Ser Thr Gly 
            20                  25                  30          


Asn Ser Ser Ile Asp Ile Ser Leu Ser Leu Val Gln Phe Leu Val Ser 
        35                  40                  45              


Asn Phe Val Pro Gly Gly Gly Phe Leu Val Gly Leu Ile Asp Phe Val 
    50                  55                  60                  


Trp Gly Ile Val Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile 
65                  70                  75                  80  


Glu Gln Leu Ile Asn Glu Arg Ile Ala Glu Phe Ala Arg Asn Ala Ala 
                85                  90                  95      


Ile Ala Asn Leu Glu Gly Leu Gly Asn Asn Phe Asn Ile Tyr Val Glu 
            100                 105                 110         


Ala Phe Lys Glu Trp Glu Glu Asp Pro Lys Asn Pro Ala Thr Arg Thr 
        115                 120                 125             


Arg Val Ile Asp Arg Phe Arg Ile Leu Asp Gly Leu Leu Glu Arg Asp 
    130                 135                 140                 


Ile Pro Ser Phe Arg Ile Ser Gly Phe Glu Val Pro Leu Leu Ser Val 
145                 150                 155                 160 


Tyr Ala Gln Ala Ala Asn Leu His Leu Ala Ile Leu Arg Asp Ser Val 
                165                 170                 175     


Ile Phe Gly Glu Arg Trp Gly Leu Thr Thr Ile Asn Val Asn Glu Asn 
            180                 185                 190         


Tyr Asn Arg Leu Ile Arg His Ile Asp Glu Tyr Ala Asp His Cys Ala 
        195                 200                 205             


Asn Thr Tyr Asn Arg Gly Leu Asn Asn Leu Pro Lys Ser Thr Tyr Gln 
    210                 215                 220                 


Asp Trp Ile Thr Tyr Asn Arg Leu Arg Arg Asp Leu Thr Leu Thr Val 
225                 230                 235                 240 


Leu Asp Ile Ala Ala Phe Phe Pro Asn Tyr Asp Asn Arg Arg Tyr Pro 
                245                 250                 255     


Ile Gln Pro Val Gly Gln Leu Thr Arg Glu Val Tyr Thr Asp Pro Leu 
            260                 265                 270         


Ile Asn Phe Asn Pro Gln Leu Gln Ser Val Ala Gln Leu Pro Thr Phe 
        275                 280                 285             


Asn Val Met Glu Asn Ser Ala Ile Arg Asn Pro His Leu Phe Asp Ile 
    290                 295                 300                 


Leu Asn Asn Leu Thr Ile Phe Thr Asp Trp Phe Ser Val Gly Arg Asn 
305                 310                 315                 320 


Phe Tyr Trp Gly Gly His Arg Val Ile Ser Ser Leu Ile Gly Gly Gly 
                325                 330                 335     


Asn Ile Thr Ser Pro Ile Tyr Gly Arg Glu Ala Asn Gln Glu Pro Pro 
            340                 345                 350         


Arg Ser Phe Thr Phe Asn Gly Pro Val Phe Arg Thr Leu Ser Asn Pro 
        355                 360                 365             


Thr Leu Arg Leu Leu Gln Gln Pro Trp Pro Ala Pro Pro Phe Asn Leu 
    370                 375                 380                 


Arg Gly Val Glu Gly Val Glu Phe Ser Thr Pro Thr Asn Ser Phe Thr 
385                 390                 395                 400 


Tyr Arg Gly Arg Gly Thr Val Asp Ser Leu Thr Glu Leu Pro Pro Glu 
                405                 410                 415     


Asp Asn Ser Val Pro Pro Arg Glu Gly Tyr Ser His Arg Leu Cys His 
            420                 425                 430         


Ala Thr Phe Val Gln Arg Ser Gly Thr Pro Phe Leu Thr Thr Gly Val 
        435                 440                 445             


Val Phe Ser Trp Thr His Arg Ser Ala Thr Leu Thr Asn Thr Ile Asp 
    450                 455                 460                 


Pro Glu Arg Ile Asn Gln Ile Pro Leu Val Lys Gly Phe Arg Val Trp 
465                 470                 475                 480 


Gly Gly Thr Ser Val Ile Thr Gly Pro Gly Phe Thr Gly Gly Asp Ile 
                485                 490                 495     


Leu Arg Arg Asn Thr Phe Gly Asp Phe Val Ser Leu Gln Val Asn Ile 
            500                 505                 510         


Asn Ser Pro Ile Thr Gln Arg Tyr Arg Leu Arg Phe Arg Tyr Ala Ser 
        515                 520                 525             


Ser Arg Asp Ala Arg Val Ile Val Leu Thr Gly Ala Ala Ser Thr Gly 
    530                 535                 540                 


Val Gly Gly Gln Val Ser Val Asn Met Pro Leu Gln Lys Thr Met Glu 
545                 550                 555                 560 


Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg Tyr Thr Asp Phe Ser 
                565                 570                 575     


Asn Pro Phe Ser Phe Arg Ala Asn Pro Asp Ile Ile Gly Ile Ser Glu 
            580                 585                 590         


Gln Pro Leu Phe Gly Ala Gly Ser Ile Ser Ser Gly Glu Leu Tyr Ile 
        595                 600                 605             


Asp Lys Ile Glu Ile Ile Leu Ala Asp Ala Thr 
    610                 615                 


<210>  3
<211>  612
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cry1Ab

<400>  3

Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu 
1               5                   10                  15      


Ser Asn Pro Glu Val Glu Val Leu Gly Gly Glu Arg Ile Glu Thr Gly 
            20                  25                  30          


Tyr Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser 
        35                  40                  45              


Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile 
    50                  55                  60                  


Trp Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile 
65                  70                  75                  80  


Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala 
                85                  90                  95      


Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr Ala Glu 
            100                 105                 110         


Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu 
        115                 120                 125             


Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Thr Thr Ala 
    130                 135                 140                 


Ile Pro Leu Phe Ala Val Gln Asn Tyr Gln Val Pro Leu Leu Ser Val 
145                 150                 155                 160 


Tyr Val Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser 
                165                 170                 175     


Val Phe Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg 
            180                 185                 190         


Tyr Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp Tyr Ala Val 
        195                 200                 205             


Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg 
    210                 215                 220                 


Asp Trp Val Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val 
225                 230                 235                 240 


Leu Asp Ile Val Ala Leu Phe Pro Asn Tyr Asp Ser Arg Arg Tyr Pro 
                245                 250                 255     


Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr Thr Asn Pro Val 
            260                 265                 270         


Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu 
        275                 280                 285             


Arg Ser Ile Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr 
    290                 295                 300                 


Ile Tyr Thr Asp Ala His Arg Gly Tyr Tyr Tyr Trp Ser Gly His Gln 
305                 310                 315                 320 


Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro 
                325                 330                 335     


Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val Ala 
            340                 345                 350         


Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Leu Tyr Arg 
        355                 360                 365             


Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val Leu Asp 
    370                 375                 380                 


Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu Pro Ser Ala Val 
385                 390                 395                 400 


Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln 
                405                 410                 415     


Asn Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His 
            420                 425                 430         


Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile 
        435                 440                 445             


Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn 
    450                 455                 460                 


Ile Ile Pro Ser Ser Gln Ile Thr Gln Ile Pro Leu Thr Lys Ser Thr 
465                 470                 475                 480 


Asn Leu Gly Ser Gly Thr Ser Val Val Lys Gly Pro Gly Phe Thr Gly 
                485                 490                 495     


Gly Asp Ile Leu Arg Arg Thr Ser Pro Gly Gln Ile Ser Thr Leu Arg 
            500                 505                 510         


Val Asn Ile Thr Ala Pro Leu Ser Gln Arg Tyr Arg Val Arg Ile Arg 
        515                 520                 525             


Tyr Ala Ser Thr Thr Asn Leu Gln Phe His Thr Ser Ile Asp Gly Arg 
    530                 535                 540                 


Pro Ile Asn Gln Gly Asn Phe Ser Ala Thr Met Ser Ser Gly Ser Asn 
545                 550                 555                 560 


Leu Gln Ser Gly Ser Phe Arg Thr Val Gly Phe Thr Thr Pro Phe Asn 
                565                 570                 575     


Phe Ser Asn Gly Ser Ser Val Phe Thr Leu Ser Ala His Val Phe Asn 
            580                 585                 590         


Ser Gly Asn Glu Val Tyr Ile Asp Arg Ile Glu Phe Val Pro Ala Glu 
        595                 600                 605             


Val Thr Phe Glu 
    610         


