
                                SEQUENCE LISTING

<110> Pioneer Hi-Bred International, Inc.

<120> Novel Bacillus Thuringiensis Gene with
  Coleopteran Activity

<130> 3570-PCT

<160> 2

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 1922
<212> DNA
<213> Bacillus Thuringiensis

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> GS 123

<400> 1
atgaattata aagattacct gagaatgtct gagggctatg atagagaata ttttgcttca 60
cctggagcac ttgttagtgg gaaacaagca attaaggttg gaatcgatat tgtcggcaaa 120
atattagggg cgttagggat tccatttgtt ggtcagatag ttggttttta taattttatt 180
ctcgatcagc tatggccatc aaattctgtg agtatatggg aacagattat gaccctagta 240
gaagaacttg tagatcaaaa aataacagaa tatgcaagaa ataaagcaat cgctgaatta 300
acaggattag gaaatgctat ggatgtatat cagcagtcac ttgaagattg ggtggcaaat 360
cctaatgacg caagaactag aagtgttgtt gctactcaat ttacagcctt agaacttgat 420
tttgttcaag caatcccatc atttgcaata tctgggcaag aggtaccatt attgggagta 480
tatgcacagg ctgtgaacct gcacttattg ttactaaggg acgcttctat atttggggaa 540
gagtggggat tctcatcatc agaaatttct cgatactata accgtcaagt gcaactcact 600
tctcaatatt ccgattattg cgtgaagtgg tacaataccg gtttacagaa attaaaaggt 660
acgagcgctg agaattggct ggagtatcat caattccgca gagagatgac tttcatggta 720
ttagatttgg ttgcattatt tccaaactac aatacacaca tgtatccact tgaaacaaag 780
gctcaactta cacgagaggt atatacggat ccgatcgcct ttaatctttc cggggcagcg 840
ggtttttgta gcccttggtc aaagtatact gttatttcct tttcggagat tgaaaatgct 900
gtaattcgtc cgcctcattt atttaatgta ctcagaagtt tagagattaa tacagttagg 960
gggacaattt taggtaatac taaagattac ctaaactatt ggtcaggtca ttctctacga 1020
tataatttta taggtgatac aacagtaagg gaaaataatt atggatatct tacttcagaa 1080
aaaactagga ttgaattaga cactagagat atttttgaga ttaattcaac tgccgcaagc 1140
ttagcgaatt actatcaaga gaaatatggt gtgccagaat cttggctcca tatggtgcaa 1200
tgggatagcc catattatac atcatcttat ctttattcta aaacacatac aactggagaa 1260
ggttgtacac aagtttatga atcaagtgag gaaatacctg tagacagaac ggtaccggta 1320
aatgaaggtt atagtcacag actatcgtat gtcacctctc ttgatttctt taaaattatt 1380
aatagttttt ctagtagcgg aaatctacct gtgtttgttt ggacacatcg gagtgcggat 1440
ttcaccaata caatttatcc ggataaaatc actcaaatac caatggtaaa ggcctatgat 1500
ttggctcccg cagctctacc agggactccg gcccctacca ttgtgccggg tcctggattt 1560
acagggggcg atatactaaa agtagtagcg agaggcgcaa tatcaaacgg tttggtactt 1620
gaagtcccag acataaacaa aaaatatatt atgagaattc ggtatgcttc ctttgcaaat 1680
cctaacttca tcatagatat acgatcaggg gaaacagtta gctctagtgc tcaaaaaact 1740
atgaattcag gggacacttt aacatataat aaatttaatt atgcaacttt cccacctgtt 1800
aaatttagtt caactaagcc taccctattt ctacgagcag attttactac tacctttgca 1860
gaaccaatgc cattcgaaac atatatagac cgaattgaat tcatcccagt agatgcgaca 1920
ta                                                                1922

<210> 2
<211> 640
<212> PRT
<213> Bacillus Thuringiensis

<220> 
<221> PEPTIDE        
<222> (0)...(0)
<223> GS 123

<400> 2
Met Asn Tyr Lys Asp Tyr Leu Arg Met Ser Glu Gly Tyr Asp Arg Glu
 1               5                  10                  15      
Tyr Phe Ala Ser Pro Gly Ala Leu Val Ser Gly Lys Gln Ala Ile Lys
            20                  25                  30          
Val Gly Ile Asp Ile Val Gly Lys Ile Leu Gly Ala Leu Gly Ile Pro
        35                  40                  45              
Phe Val Gly Gln Ile Val Gly Phe Tyr Asn Phe Ile Leu Asp Gln Leu
    50                  55                  60                  
Trp Pro Ser Asn Ser Val Ser Ile Trp Glu Gln Ile Met Thr Leu Val
65                  70                  75                  80  
Glu Glu Leu Val Asp Gln Lys Ile Thr Glu Tyr Ala Arg Asn Lys Ala
                85                  90                  95      
Ile Ala Glu Leu Thr Gly Leu Gly Asn Ala Met Asp Val Tyr Gln Gln
            100                 105                 110         
Ser Leu Glu Asp Trp Val Ala Asn Pro Asn Asp Ala Arg Thr Arg Ser
        115                 120                 125             
Val Val Ala Thr Gln Phe Thr Ala Leu Glu Leu Asp Phe Val Gln Ala
    130                 135                 140                 
Ile Pro Ser Phe Ala Ile Ser Gly Gln Glu Val Pro Leu Leu Gly Val
145                 150                 155                 160 
Tyr Ala Gln Ala Val Asn Leu His Leu Leu Leu Leu Arg Asp Ala Ser
                165                 170                 175     
Ile Phe Gly Glu Glu Trp Gly Phe Ser Ser Ser Glu Ile Ser Arg Tyr
            180                 185                 190         
Tyr Asn Arg Gln Val Gln Leu Thr Ser Gln Tyr Ser Asp Tyr Cys Val
        195                 200                 205             
Lys Trp Tyr Asn Thr Gly Leu Gln Lys Leu Lys Gly Thr Ser Ala Glu
    210                 215                 220                 
Asn Trp Leu Glu Tyr His Gln Phe Arg Arg Glu Met Thr Phe Met Val
225                 230                 235                 240 
Leu Asp Leu Val Ala Leu Phe Pro Asn Tyr Asn Thr His Met Tyr Pro
                245                 250                 255     
Leu Glu Thr Lys Ala Gln Leu Thr Arg Glu Val Tyr Thr Asp Pro Ile
            260                 265                 270         
Ala Phe Asn Leu Ser Gly Ala Ala Gly Phe Cys Ser Pro Trp Ser Lys
        275                 280                 285             
Tyr Thr Val Ile Ser Phe Ser Glu Ile Glu Asn Ala Val Ile Arg Pro
    290                 295                 300                 
Pro His Leu Phe Asn Val Leu Arg Ser Leu Glu Ile Asn Thr Val Arg
305                 310                 315                 320 
Gly Thr Ile Leu Gly Asn Thr Lys Asp Tyr Leu Asn Tyr Trp Ser Gly
                325                 330                 335     
His Ser Leu Arg Tyr Asn Phe Ile Gly Asp Thr Thr Val Arg Glu Asn
            340                 345                 350         
Asn Tyr Gly Tyr Leu Thr Ser Glu Lys Thr Arg Ile Glu Leu Asp Thr
        355                 360                 365             
Arg Asp Ile Phe Glu Ile Asn Ser Thr Ala Ala Ser Leu Ala Asn Tyr
    370                 375                 380                 
Tyr Gln Glu Lys Tyr Gly Val Pro Glu Ser Trp Leu His Met Val Gln
385                 390                 395                 400 
Trp Asp Ser Pro Tyr Tyr Thr Ser Ser Tyr Leu Tyr Ser Lys Thr His
                405                 410                 415     
Thr Thr Gly Glu Gly Cys Thr Gln Val Tyr Glu Ser Ser Glu Glu Ile
            420                 425                 430         
Pro Val Asp Arg Thr Val Pro Val Asn Glu Gly Tyr Ser His Arg Leu
        435                 440                 445             
Ser Tyr Val Thr Ser Leu Asp Phe Phe Lys Ile Ile Asn Ser Phe Ser
    450                 455                 460                 
Ser Ser Gly Asn Leu Pro Val Phe Val Trp Thr His Arg Ser Ala Asp
465                 470                 475                 480 
Phe Thr Asn Thr Ile Tyr Pro Asp Lys Ile Thr Gln Ile Pro Met Val
                485                 490                 495     
Lys Ala Tyr Asp Leu Ala Pro Ala Ala Leu Pro Gly Thr Pro Ala Pro
            500                 505                 510         
Thr Ile Val Pro Gly Pro Gly Phe Thr Gly Gly Asp Ile Leu Lys Val
        515                 520                 525             
Val Ala Arg Gly Ala Ile Ser Asn Gly Leu Val Leu Glu Val Pro Asp
    530                 535                 540                 
Ile Asn Lys Lys Tyr Ile Met Arg Ile Arg Tyr Ala Ser Phe Ala Asn
545                 550                 555                 560 
Pro Asn Phe Ile Ile Asp Ile Arg Ser Gly Glu Thr Val Ser Ser Ser
                565                 570                 575     
Ala Gln Lys Thr Met Asn Ser Gly Asp Thr Leu Thr Tyr Asn Lys Phe
            580                 585                 590         
Asn Tyr Ala Thr Phe Pro Pro Val Lys Phe Ser Ser Thr Lys Pro Thr
        595                 600                 605             
Leu Phe Leu Arg Ala Asp Phe Thr Thr Thr Phe Ala Glu Pro Met Pro
    610                 615                 620                 
Phe Glu Thr Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Asp Ala Thr
625                 630                 635                 640 

