                         SEQUENCE LISTING

<110>  THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE 
       SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES
       UNIVERSITY OF MARYLAND, COLLEGE PARK
 
<120>  DELIVERY OF TRANSTHYRETIN ACROSS THE BLOOD-BRAIN BARRIER AS A 
       TREATMENT FOR ALZHEIMER'S DISEASE

<130>  707050

<150>  US 61/286,205
<151>  2009-12-14

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  1
ccgggaataa tcaaaagatt gttaacctga aagagaaggt agcccagctt gaagcaa          57


<210>  2
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  2
ctagttgctt caagctgggc taccttctct ttcaggttaa caatcttttg attattc          57


<210>  3
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  3
actagttctt cttcttctgg ctcttcttct tctggcgaat tc                          42


<210>  4
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  4
gaattcgcca gaagaagaag agccagaaga agaagaacta gt                          42


<210>  5
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  5
aattcgcttc tcatcgtctg ctcc                                              24


<210>  6
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  6
tcgagaagga accctaacca ctgctg                                            26


<210>  7
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  7

Asn Asn Gln Lys Ile Val Asn Leu Lys Glu Lys Val Ala Gln Leu Glu 
1               5                   10                  15      


Ala 
    


<210>  8
<211>  1110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  8
atgaaaatcg aagaaggtaa actgacaaat cctggtgtat ccgcttggca ggtcaacaca       60

gcttatactg cgggacaatt ggtcacatat aacggcaaga cgtataaatg tttgcagccc      120

cacacctcct tggcaggatg ggaaccatcc aacgttcctg ccttgtggca gcttcaagct      180

atctctggcg atagtctgat cagcctggct agcacaggaa aaagagtttc tattaaagat      240

ttgttagatg aaaaagattt tgaaatatgg gcaattaatg aacagacgat gaagctagaa      300

tcagctaaag ttagtcgtgt attttgtact ggcaaaaagc tagtttatat tctaaaaact      360

cgactaggta gaactatcaa ggcaacagca aatcatagat ttttaactat tgatggttgg      420

aaaagattag atgagctatc tttaaaagag catattgctc taccccgtaa actagaaagc      480

tcctctttac aattgtcacc agaaatagaa aagttgtctc agagtgatat ttactgggac      540

tccatcgttt ctattacgga gactggagtc gaagaggttt ttgatttgac tgtgccagga      600

ccacataact ttgtcgcgaa tgacatcatt gtacacaaca ataatcaaaa gatcgttaac      660

cttaaagaga aggtagccca gcttgaagca tcgtcgtcgt cgggctcgtc gtcgtcgggc      720

gcagcaggcc ctacgggcac cggtgaatcc aagtgtcctc tgatggtcaa agttctagat      780

gctgtccgag gcagtcctgc catcaatgtg gccgtgcatg tgttcagaaa ggctgctgat      840

gacacctggg agccatttgc ctctgggaaa accagtgagt ctggagagct gcatgggctc      900

acaactgagg aggaatttgt agaagggata tacaaagtgg aaatagacac caaatcttac      960

tggaaggcac ttggcatctc cccattccat gagcatgcag aggtggtatt cacagccaac     1020

gactccggcc cccgccgcta caccattgcc gccctgctga gcccctactc ctattccacc     1080

acggctgtcg tcaccaatcc caaggaatga                                      1110


<210>  9
<211>  369
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  9

Met Lys Ile Glu Glu Gly Lys Leu Thr Asn Pro Gly Val Ser Ala Trp 
1               5                   10                  15      


Gln Val Asn Thr Ala Tyr Thr Ala Gly Gln Leu Val Thr Tyr Asn Gly 
            20                  25                  30          


Lys Thr Tyr Lys Cys Leu Gln Pro His Thr Ser Leu Ala Gly Trp Glu 
        35                  40                  45              


Pro Ser Asn Val Pro Ala Leu Trp Gln Leu Gln Ala Ile Ser Gly Asp 
    50                  55                  60                  


Ser Leu Ile Ser Leu Ala Ser Thr Gly Lys Arg Val Ser Ile Lys Asp 
65                  70                  75                  80  


Leu Leu Asp Glu Lys Asp Phe Glu Ile Trp Ala Ile Asn Glu Gln Thr 
                85                  90                  95      


Met Lys Leu Glu Ser Ala Lys Val Ser Arg Val Phe Cys Thr Gly Lys 
            100                 105                 110         


Lys Leu Val Tyr Ile Leu Lys Thr Arg Leu Gly Arg Thr Ile Lys Ala 
        115                 120                 125             


Thr Ala Asn His Arg Phe Leu Thr Ile Asp Gly Trp Lys Arg Leu Asp 
    130                 135                 140                 


Glu Leu Ser Leu Lys Glu His Ile Ala Leu Pro Arg Lys Leu Glu Ser 
145                 150                 155                 160 


Ser Ser Leu Gln Leu Ser Pro Glu Ile Glu Lys Leu Ser Gln Ser Asp 
                165                 170                 175     


Ile Tyr Trp Asp Ser Ile Val Ser Ile Thr Glu Thr Gly Val Glu Glu 
            180                 185                 190         


Val Phe Asp Leu Thr Val Pro Gly Pro His Asn Phe Val Ala Asn Asp 
        195                 200                 205             


Ile Ile Val His Asn Asn Asn Gln Lys Ile Val Asn Leu Lys Glu Lys 
    210                 215                 220                 


Val Ala Gln Leu Glu Ala Ser Ser Ser Ser Gly Ser Ser Ser Ser Gly 
225                 230                 235                 240 


Ala Ala Gly Pro Thr Gly Thr Gly Glu Ser Lys Cys Pro Leu Met Val 
                245                 250                 255     


Lys Val Leu Asp Ala Val Arg Gly Ser Pro Ala Ile Asn Val Ala Val 
            260                 265                 270         


His Val Phe Arg Lys Ala Ala Asp Asp Thr Trp Glu Pro Phe Ala Ser 
        275                 280                 285             


Gly Lys Thr Ser Glu Ser Gly Glu Leu His Gly Leu Thr Thr Glu Glu 
    290                 295                 300                 


Glu Phe Val Glu Gly Ile Tyr Lys Val Glu Ile Asp Thr Lys Ser Tyr 
305                 310                 315                 320 


Trp Lys Ala Leu Gly Ile Ser Pro Phe His Glu His Ala Glu Val Val 
                325                 330                 335     


Phe Thr Ala Asn Asp Ser Gly Pro Arg Arg Tyr Thr Ile Ala Ala Leu 
            340                 345                 350         


Leu Ser Pro Tyr Ser Tyr Ser Thr Thr Ala Val Val Thr Asn Pro Lys 
        355                 360                 365             


Glu 
    


<210>  10
<211>  156
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  10

Asn Asn Gln Lys Ile Val Asn Leu Lys Glu Lys Val Ala Gln Leu Glu 
1               5                   10                  15      


Ala Ser Ser Ser Ser Gly Ser Ser Ser Ser Gly Ala Ala Gly Pro Thr 
            20                  25                  30          


Gly Thr Gly Glu Ser Lys Cys Pro Leu Met Val Lys Val Leu Asp Ala 
        35                  40                  45              


Val Arg Gly Ser Pro Ala Ile Asn Val Ala Val His Val Phe Arg Lys 
    50                  55                  60                  


Ala Ala Asp Asp Thr Trp Glu Pro Phe Ala Ser Gly Lys Thr Ser Glu 
65                  70                  75                  80  


Ser Gly Glu Leu His Gly Leu Thr Thr Glu Glu Glu Phe Val Glu Gly 
                85                  90                  95      


Ile Tyr Lys Val Glu Ile Asp Thr Lys Ser Tyr Trp Lys Ala Leu Gly 
            100                 105                 110         


Ile Ser Pro Phe His Glu His Ala Glu Val Val Phe Thr Ala Asn Asp 
        115                 120                 125             


Ser Gly Pro Arg Arg Tyr Thr Ile Ala Ala Leu Leu Ser Pro Tyr Ser 
    130                 135                 140                 


Tyr Ser Thr Thr Ala Val Val Thr Asn Pro Lys Glu 
145                 150                 155     


<210>  11
<211>  1110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  11
atgaaaatcg aagaaggtaa actgacaaat cctggtgtat ccgcttggca ggtcaacaca       60

gcttatactg cgggacaatt ggtcacatat aacggcaaga cgtataaatg tttgcagccc      120

cacacctcct tggcaggatg ggaaccatcc aacgttcctg ccttgtggca gcttcaagct      180

atctctggcg atagtctgat cagcctggct agcacaggaa aaagagtttc tattaaagat      240

ttgttagatg aaaaagattt tgaaatatgg gcaattaatg aacagacgat gaagctagaa      300

tcagctaaag ttagtcgtgt attttgtact ggcaaaaagc tagtttatat tctaaaaact      360

cgactaggta gaactatcaa ggcaacagca aatcatagat ttttaactat tgatggttgg      420

aaaagattag atgagctatc tttaaaagag catattgctc taccccgtaa actagaaagc      480

tcctctttac aattgtcacc agaaatagaa aagttgtctc agagtgatat ttactgggac      540

tccatcgttt ctattacgga gactggagtc gaagaggttt ttgatttgac tgtgccagga      600

ccacataact ttgtcgcgaa tgacatcatt gtacacaaca ataatcaaaa gcttgttaac      660

atcaaagaga aggtagccca gatcgaagca tcgtcgtcgt cgggctcgtc gtcgtcgggc      720

gcagcaggcc ctacgggcac cggtgaatcc aagtgtcctc tgatggtcaa agttctagat      780

gctgtccgag gcagtcctgc catcaatgtg gccgtgcatg tgttcagaaa ggctgctgat      840

gacacctggg agccatttgc ctctgggaaa accagtgagt ctggagagct gcatgggctc      900

acaactgagg aggaatttgt agaagggata tacaaagtgg aaatagacac caaatcttac      960

tggaaggcac ttggcatctc cccattccat gagcatgcag aggtggtatt cacagccaac     1020

gactccggcc cccgccgcta caccattgcc gccctgctga gcccctactc ctattccacc     1080

acggctgtcg tcaccaatcc caaggaatga                                      1110


<210>  12
<211>  369
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  12

Met Lys Ile Glu Glu Gly Lys Leu Thr Asn Pro Gly Val Ser Ala Trp 
1               5                   10                  15      


Gln Val Asn Thr Ala Tyr Thr Ala Gly Gln Leu Val Thr Tyr Asn Gly 
            20                  25                  30          


Lys Thr Tyr Lys Cys Leu Gln Pro His Thr Ser Leu Ala Gly Trp Glu 
        35                  40                  45              


Pro Ser Asn Val Pro Ala Leu Trp Gln Leu Gln Ala Ile Ser Gly Asp 
    50                  55                  60                  


Ser Leu Ile Ser Leu Ala Ser Thr Gly Lys Arg Val Ser Ile Lys Asp 
65                  70                  75                  80  


Leu Leu Asp Glu Lys Asp Phe Glu Ile Trp Ala Ile Asn Glu Gln Thr 
                85                  90                  95      


Met Lys Leu Glu Ser Ala Lys Val Ser Arg Val Phe Cys Thr Gly Lys 
            100                 105                 110         


Lys Leu Val Tyr Ile Leu Lys Thr Arg Leu Gly Arg Thr Ile Lys Ala 
        115                 120                 125             


Thr Ala Asn His Arg Phe Leu Thr Ile Asp Gly Trp Lys Arg Leu Asp 
    130                 135                 140                 


Glu Leu Ser Leu Lys Glu His Ile Ala Leu Pro Arg Lys Leu Glu Ser 
145                 150                 155                 160 


Ser Ser Leu Gln Leu Ser Pro Glu Ile Glu Lys Leu Ser Gln Ser Asp 
                165                 170                 175     


Ile Tyr Trp Asp Ser Ile Val Ser Ile Thr Glu Thr Gly Val Glu Glu 
            180                 185                 190         


Val Phe Asp Leu Thr Val Pro Gly Pro His Asn Phe Val Ala Asn Asp 
        195                 200                 205             


Ile Ile Val His Asn Asn Asn Gln Lys Leu Val Asn Ile Lys Glu Lys 
    210                 215                 220                 


Val Ala Gln Ile Glu Ala Ser Ser Ser Ser Gly Ser Ser Ser Ser Gly 
225                 230                 235                 240 


Ala Ala Gly Pro Thr Gly Thr Gly Glu Ser Lys Cys Pro Leu Met Val 
                245                 250                 255     


Lys Val Leu Asp Ala Val Arg Gly Ser Pro Ala Ile Asn Val Ala Val 
            260                 265                 270         


His Val Phe Arg Lys Ala Ala Asp Asp Thr Trp Glu Pro Phe Ala Ser 
        275                 280                 285             


Gly Lys Thr Ser Glu Ser Gly Glu Leu His Gly Leu Thr Thr Glu Glu 
    290                 295                 300                 


Glu Phe Val Glu Gly Ile Tyr Lys Val Glu Ile Asp Thr Lys Ser Tyr 
305                 310                 315                 320 


Trp Lys Ala Leu Gly Ile Ser Pro Phe His Glu His Ala Glu Val Val 
                325                 330                 335     


Phe Thr Ala Asn Asp Ser Gly Pro Arg Arg Tyr Thr Ile Ala Ala Leu 
            340                 345                 350         


Leu Ser Pro Tyr Ser Tyr Ser Thr Thr Ala Val Val Thr Asn Pro Lys 
        355                 360                 365             


Glu 
    


<210>  13
<211>  156
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  13

Asn Asn Gln Lys Leu Val Asn Ile Lys Glu Lys Val Ala Gln Ile Glu 
1               5                   10                  15      


Ala Ser Ser Ser Ser Gly Ser Ser Ser Ser Gly Ala Ala Gly Pro Thr 
            20                  25                  30          


Gly Thr Gly Glu Ser Lys Cys Pro Leu Met Val Lys Val Leu Asp Ala 
        35                  40                  45              


Val Arg Gly Ser Pro Ala Ile Asn Val Ala Val His Val Phe Arg Lys 
    50                  55                  60                  


Ala Ala Asp Asp Thr Trp Glu Pro Phe Ala Ser Gly Lys Thr Ser Glu 
65                  70                  75                  80  


Ser Gly Glu Leu His Gly Leu Thr Thr Glu Glu Glu Phe Val Glu Gly 
                85                  90                  95      


Ile Tyr Lys Val Glu Ile Asp Thr Lys Ser Tyr Trp Lys Ala Leu Gly 
            100                 105                 110         


Ile Ser Pro Phe His Glu His Ala Glu Val Val Phe Thr Ala Asn Asp 
        115                 120                 125             


Ser Gly Pro Arg Arg Tyr Thr Ile Ala Ala Leu Leu Ser Pro Tyr Ser 
    130                 135                 140                 


Tyr Ser Thr Thr Ala Val Val Thr Asn Pro Lys Glu 
145                 150                 155     


