                         SEQUENCE LISTING

<110>  Keygene NV
 
<120>  Improved techniques for transfecting protoplasts

<130>  P30039PC00

<150>  US 61/288474
<151>  2009-12-21

<150>  NL2004020
<151>  2009-12-24

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  776
<212>  DNA
<213>  Lycopersicon esculentum


<220>
<221>  misc_feature
<223>  KU70

<400>  1
ggaagatctg aacgaccagc ttaggaaacg catgtttaag aagcgcagag ttcgaagact       60

tcgacttgta atttttaatg gattatctat cgaacttaac acctatgctt tgatccgtcc      120

aactaatcca gggacaatta cttggcttga ttcgatgact aatcttcctt tgaagactga      180

gagaaccttc atatgtgctg atactggtgc tatagttcag gagcctctaa aacgctttca      240

gtcttacaaa aatgagaatg tcatcttttc tgcggatgag ctttcagaag tcaaaagagt      300

ttcaactgga catcttcgtc tgttgggctt caagcctttg agctgcttaa aagactatca      360

taacctgaag ccagcaactt ttgtctttcc cagtgatgag gaagtggttg gaagcacttg      420

tcttttcgtt gctctccaaa gatcaatgtt gcggcttaag cgttttgcag ttgctttcta      480

tgggaattta agtcatcctc aattggttgc tcttgttgca caagatgaag taatgactcc      540

tagtggtcaa gtcgagccac cagggatgca tctgatttat cttccatatt ctgatgatat      600

cagacatgtt gaagagcttc atactgatcc taattccgtg cctcatgcca ctgatgacca      660

gataaagaag gcctccgctt tagtgagacg tattgacctc aaagattttt ctgtgtggca      720

atttgctaat cctgcattgc agagacatta tgcagtatta caagctcttg cacttg          776


<210>  2
<211>  884
<212>  DNA
<213>  Nicotiana benthamiana

<400>  2
ggagctactg atagatcatt gattataatt gatgagttgg gccgtggtac atcaacctat       60

gatggctttg gtttagcttg ggctatttgt gagcacattg ttgaagaaat taaggcacca      120

acattgtttg ccactcactt tcatgagctg actgcattgg ccaacaagaa tggtaacaat      180

ggacataagc aaaatgctgg gatagcaaat tttcatgttt ttgcacacat tgacccttct      240

aatcgcaagc taactatgct ttacaaggtt caaccaggtg cttgtgatca gagttttggt      300

attcatgttg ctgaatttgc aaattttcca ccgagtgttg tggccctggc cagagaaaag      360

gcatctgagt tggaggattt ctctcctatt gccataattc caaatgacat taaagaggca      420

gcttcaaaac ggaagagaga atttgaccct catgacgtgt ctagaggtac tgccagagct      480

cggcaattct tacaggattt ctctcagttg ccactggata agatggatcc aagcgaggtc      540

aggcaacagt tgagcaaaat gaaaaccgac ctggagaggg atgcagttga ctctcactgg      600

tttcagcaat tcttttagtt cttcagatta gaactatatc ttctattctg tgaagcttgg      660

gggaatgata gtgatgggtt ttgtggatat aacttagcct aagtgtaaag tttcgtttaa      720

atccttaccc caaacatgat tctctgtaat caggggactt ttgtatgcat cctgtgttaa      780

atagtaaacg ttatcttatg gtcagctaac attggtagta gtctattgaa ttattccttc      840

acaacgacta aacaaccttc ccttctctta aaacacccta aact                       884


<210>  3
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  3
accagcttag gaaacgca                                                     18


<210>  4
<211>  18
<212>  DNA
<213>  primer

<400>  4
agcaccagta tcagcaca                                                     18


<210>  5
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  5
cacacattga cccttctaat cgc                                               23


<210>  6
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  6
agaaatcctc caactcagat gcc                                               23


<210>  7
<211>  786
<212>  DNA
<213>  Nicotiana benthamiana

<400>  7
atgggaagag gatcgcatca ccaccatcat cataagcttc caaagaagaa gaggaaggtt       60

ctcgagatgg tgagcaaggg ctaggagctg ttcaccgggg tggtgcccat cctggtcgag      120

ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc      180

acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg      240

cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac      300

atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc      360

atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac      420

accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg      480

gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag      540

aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag      600

ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac      660

aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac      720

atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac      780

aagtaa                                                                 786


<210>  8
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  mutagenic oligonucleotide


<220>
<221>  modified_base
<222>  (1)..(4)
<223>  phosphorothioate modified nucleotides

<220>
<221>  modified_base
<222>  (22)..(25)
<223>  phosphorothioate modified nucleotides

<400>  8
tgaacagctc ctcgcccttg ctcac                                             25


<210>  9
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  mutagenic oligonucleotide


<220>
<221>  modified_base
<222>  (1)..(4)
<223>  phophorothioate modified nucleotides

<220>
<221>  modified_base
<222>  (22)..(25)
<223>  phophorothioate modified nucleotides

<400>  9
tgaacagctc ctagcccttg ctcac                                             25


