                         SEQUENCE LISTING

<110>  Health Protection Agency
 
<120>  Transformation of commensal Neisseria

<130>  P33873WO

<150>  GB 0922224.1
<151>  2009-12-21

<160>  12    

<170>  PatentIn version 3.2

<210>  1
<211>  1057
<212>  DNA
<213>  N. lactamica

<400>  1
aaaatcgaaa ccccgtgaag cgggcaactt cacggggttc tgtatttaat ccttatgggc     60

aaggagaaaa tcataagtgg attttaacgt aaacacggag gcaaggaaag tggcagaact    120

gttggaaaaa tcaaaaacac tccgccgcct tgcatggggt ttgatactga tgaccccgac    180

aacggcgctg atatggaaat tggcagatat tctgaccgcg ctgaaatagg ccgtctgaaa    240

agcgaaaccc cgtgaggcgc ggcgcaattt ttggcacttc gtggcaggga tttgtctgat    300

catcacggta tggaaaatat ttaccgtgat cgaactgctt gtgaaataaa ggtttgttat    360

ggcttggaac tcaatcggca ttccgaatgt gccgaatctc ccaagaaaca ccggcggcgc    420

gctgattaag ttcggcggtg cggcactcgc caacgccgtt ttcggcaact attggggcat    480

cttcggacaa aacggcatcc cgctgctgtt gtcggacaac gtcacttcgg tcaaacacca    540

aaacacgtcc aaagtatcca atgcgcctgt cgaacgcggc tcgttcgcca gctacaacaa    600

ggttggcgac ccgtttacgg taacggtgca gatgagcaag ggcagcggcg gcgtgttcga    660

gcgcggggcg tttctcggcc tgttggatac gctggccaac agcacggatc tgttcttggt    720

tatcacgccg gaagcggtgt atccgaatat ggcgattacg ggctacgact acgcccgcga    780

ggcttcagac ggcgcacggc tgttgaaggt caatatccat ttggccgaag tgcggcaggc    840

ggaagtgaaa tataccaaaa ccaaatccga aggcgcacag gcacaggcag acggaggcag    900

ggtacagccc aagccgatac aaaacaatga atccatcttg tctaagtcgc gcggttcggt    960

cggcggttgg cttgggaaaa taaccgatac attcggaaag gggttcgggc cggattaaaa   1020

aatcgaaacc ccgtgaagcg ggcaacttca cggggtt                            1057


<210>  2
<211>  1057
<212>  DNA
<213>  N. lactamica

<400>  2
aaaagcgaaa ccccgtgaag cgtgcaactt cgcggggttc tgtatttaat ccttatgggc     60

aaggagaaaa tcataagtgg attttaacgt aaacacggag gcaaggaaag tggcagaact    120

gttggaaaaa tcaaaaacac tccgccgcct tgcatggggt ttgatactga tgaccccgac    180

aacggcgctg atatggaaat tggcagatat tctgaccgcg ctgaaatagg ccgtctgaaa    240

agcgaaaccc cgtgaggcgc ggcgcaattt ttggcacttc gtggcaggga tttgtctgat    300

catcacggta tggaaaatat ttaccgtgat cgaactgctt gtgaaataaa ggtttgttat    360

ggcttggaac tcaatcggca ttccgaatgt gccgaatctc ccaagaaaca ccggcggcgc    420

gctgattaag ttcggcggtg cggcactcgc caacgccgtt ttcggcaact attggggcat    480

cttcggacaa aacggcatcc cgctgctgtt gtcggacaac gtcacttcgg tcaaacacca    540

aaacacgtcc aaagtatcca atgcgcctgt cgaacgcggc tcgttcgcca gctacaacaa    600

ggttggcgac ccgtttacgg taacggtgca gatgagcaag ggcagcggcg gcgtgttcga    660

gcgcggggcg tttctcggcc tgttggatac gctggccaac agcacggatc tgttcttggt    720

tatcacgccg gaagcggtgt atccgaatat ggcgattacg ggctacgact acgcccgcga    780

ggcttcagac ggcgcacggc tgttgaaggt caatatccat ttggccgaag tgcggcaggc    840

ggaagtgaaa tataccaaaa ccaaatccga aggcgcacag gcacaggcag acggaggcag    900

ggtacagccc aagccgatac aaaacaatga atccatcttg tctaagtcgc gcggttcggt    960

cggcggttgg cttgggaaaa taaccgatac attcggaaag gggttcgggc cggattaaaa   1020

aatcgaaacc ccgtgaagcg ggcaacttca cggggtt                            1057


<210>  3
<211>  660
<212>  DNA
<213>  N. lactamica

<400>  3
atggcttgga actcaatcgg cattccgaat gtgccgaatc tcccaagaaa caccggcggc     60

gcgctgatta agttcggcgg tgcggcactc gccaacgccg ttttcggcaa ctattggggc    120

atcttcggac aaaacggcat cccgctgctg ttgtcggaca acgtcacttc ggtcaaacac    180

caaaacacgt ccaaagtatc caatgcgcct gtcgaacgcg gctcgttcgc cagctacaac    240

aaggttggcg acccgtttac ggtaacggtg cagatgagca agggcagcgg cggcgtgttc    300

gagcgcgggg cgtttctcgg cctgttggat acgctggcca acagcacgga tctgttcttg    360

gttatcacgc cggaagcggt gtatccgaat atggcgatta cgggctacga ctacgcccgc    420

gaggcttcag acggcgcacg gctgttgaag gtcaatatcc atttggccga agtgcggcag    480

gcggaagtga aatataccaa aaccaaatcc gaaggcgcac aggcacaggc agacggaggc    540

agggtacagc ccaagccgat acaaaacaat gaatccatct tgtctaagtc gcgcggttcg    600

gtcggcggtt ggcttgggaa aataaccgat acattcggaa aggggttcgg gccggattaa    660


<210>  4
<211>  1057
<212>  DNA
<213>  Artificial sequence

<220>
<223>  derivative of SEQ ID NO: 1 with a BamHI and a HindIII site 
       arising from single-nucleotide substitutions at positions 555 and
       602

<400>  4
aaaatcgaaa ccccgtgaag cgggcaactt cacggggttc tgtatttaat ccttatgggc     60

aaggagaaaa tcataagtgg attttaacgt aaacacggag gcaaggaaag tggcagaact    120

gttggaaaaa tcaaaaacac tccgccgcct tgcatggggt ttgatactga tgaccccgac    180

aacggcgctg atatggaaat tggcagatat tctgaccgcg ctgaaatagg ccgtctgaaa    240

agcgaaaccc cgtgaggcgc ggcgcaattt ttggcacttc gtggcaggga tttgtctgat    300

catcacggta tggaaaatat ttaccgtgat cgaactgctt gtgaaataaa ggtttgttat    360

ggcttggaac tcaatcggca ttccgaatgt gccgaatctc ccaagaaaca ccggcggcgc    420

gctgattaag ttcggcggtg cggcactcgc caacgccgtt ttcggcaact attggggcat    480

cttcggacaa aacggcatcc cgctgctgtt gtcggacaac gtcacttcgg tcaaacacca    540

aaacacgtcc aaaggatcca atgcgcctgt cgaacgcggc tcgttcgcca gctacaacaa    600

gcttggcgac ccgtttacgg taacggtgca gatgagcaag ggcagcggcg gcgtgttcga    660

gcgcggggcg tttctcggcc tgttggatac gctggccaac agcacggatc tgttcttggt    720

tatcacgccg gaagcggtgt atccgaatat ggcgattacg ggctacgact acgcccgcga    780

ggcttcagac ggcgcacggc tgttgaaggt caatatccat ttggccgaag tgcggcaggc    840

ggaagtgaaa tataccaaaa ccaaatccga aggcgcacag gcacaggcag acggaggcag    900

ggtacagccc aagccgatac aaaacaatga atccatcttg tctaagtcgc gcggttcggt    960

cggcggttgg cttgggaaaa taaccgatac attcggaaag gggttcgggc cggattaaaa   1020

aatcgaaacc ccgtgaagcg ggcaacttca cggggtt                            1057


<210>  5
<211>  2748
<212>  DNA
<213>  N. meningitidis

<400>  5
atgcaacagc aacatttgtt ccgattcaat attttatgcc tgtctttaat gactgcgctg     60

cccgcttatg cagaaaatgt gcaagccgga caagcacagg aaaaacagtt ggataccata    120

caggtaaaag ccaaaaaaca gaaaacccgc cgcgataacg aagtaaccgg gctgggcaag    180

ttggtcaagt cttccgatac gctaagtaaa gaacaggttt tgaatatccg agacctgacc    240

cgttatgatc cgggtattgc cgtggtcgaa cagggtcggg gcgcaagttc cggctattca    300

atacgcggca tggataaaaa ccgcgtttcc ttaacggtgg acggcgtttc gcaaatacag    360

tcctacaccg cgcaggcggc attgggcggg acgaggacgg cgggcagcag cggcgcaatc    420

aatgaaatcg agtatgaaaa cgtcaaagct gtcgaaatca gcaaaggctc aaactcggtc    480

gaacaaggca gcggcgcatt ggcgggctcg gtcgcatttc aaaccaaaac cgccgacgat    540

gttatcgggg aaggcaggca gtggggcatt cagagtaaaa ccgcctattc cggcaaaaac    600

cgggggctta cccaatccat cgcgctggcg gggcgcatcg gcggtgcgga ggctttgctg    660

atccacaccg ggcggcgcgc gggggaaatc cgcgcccacg aagatgcagg acgcggcgtt    720

cagagcttta acaggctggt gccggttgaa gacagcagca attacgccta tttcatcgtt    780

aaagaagaat gcaaaaacgg gagttatgaa acgtgtaaag cgaatccgaa aaaagatgtt    840

gtcggcaaag acgaacgtca aacggtttcc acccgagact acacgggtcc caaccgcttc    900

ctcgccgatc cgctttcata cgaaagccgg tcgtggctgt tccgcccggg ttttcgtttt    960

gagaataagc ggcactacat cggcggcata ctcgaacaca cgcaacaaac tttcgacacg   1020

cgcgatatga cggttccggc attcctgacc aaggcggttt ttgatgcaaa taaaaaacag   1080

gcgggttctt tgcccggtaa cggcaaatac gcgggcaacc acaaatacgg cggactgttt   1140

accaacggcg aaaacggtgc gctggtgggc gcggaatacg gtacgggcgt gttttacgac   1200

gagacgcaca ccaaaagccg ctacggtttg gaatatgtct ataccaatgc cgataaagac   1260

acttgggcgg attatgcccg cctctcttac gaccggcagg gcgtcggttt ggataatcat   1320

tttcagcaga cgcactgttc tgccgacggt tcggacaaat attgccgccc gagtgccgac   1380

aagccgtttt cctattacaa atccgatcgc gtgatttacg gggaaagcca caggctcttg   1440

caggcggcat tcaaaaaatc cttcgatacc gccaaaatcc gccacaacct gagcgtgaat   1500

ctcgggtttg accgctttgg ctctaatctc cgccatcagg attattatta tcaacatgcc   1560

aaccgcgcct attcgtcgaa cacgccccct caaaacaacg gcaaaaaaat cagccccaac   1620

ggcagtgaaa ccagccccta ttgggtcacc ataggcaggg gaaatgtcgt tacggggcaa   1680

atctgccgct tgggcaacaa tacttatacg gactgcacgc cgcgcagcat caacggtaaa   1740

agctattacg cggcagttcg ggacaatgtc cgtttgggca ggtgggcgga tgtcggcgcg   1800

ggcttgcgct acgactaccg cagcacgcat tcggacgacg gcagcgtttc caccggcacg   1860

caccgcacct tgtcctggaa cgccggcatc gtcctcaaac ctaccgactg gctggatttg   1920

acttaccgca cctcaaccgg cttccgcctg ccctcgtttg cggaaatgta cggctggcgg   1980

gcgggtgttc aaagcaaggc ggtcaaaatc gatccggaaa aatcgttcaa caaagaagcc   2040

ggcatcgtgt ttaaaggcga tttcggcaac ttggaggcaa gttggttcaa caatgcctac   2100

cgcgatttga ttgtccgggg ttatgaagcg caaattaaag acggcaaaga agaagccaaa   2160

ggcgacccgg cttacctcaa tgcccaaagc gcgcggatta ccggcatcaa tattttgggc   2220

aaaatcgatt ggaacggcgt atgggataaa ttgcccgaag gttggtattc tacatttgcc   2280

tataatcgtg tccgtgtccg cgacatcaaa aaacgcgcag accgcaccga tattcaatca   2340

catctgtttg atgccatcca accctcgcgc tatgtcgtcg gcttgggcta tgaccaaccg   2400

gaaggcaaat ggggtgtgaa cggtatgctg acttattcca aagccaagga aatcacagag   2460

ttgttgggca gccgggcttt gctcaacggc aacagccgca atacaaaagc caccgcgcgc   2520

cgtacccgcc cttggtatat tgtggacgtg tccggttatt acacggttaa aaaacacttt   2580

accctccgtg cgggcgtgta caacctcctc aactaccgct atgttacttg ggaaaatgtg   2640

cggcaaactg ccggcggcgc agtcaaccaa cacaaaaatg tcggcgttta caaccgatat   2700

gccgcccccg gtcgcaacta cacatttagc ttggaaatga agttttaa                2748


<210>  6
<211>  963
<212>  DNA
<213>  N. meningitidis

<400>  6
atgccgtctg aaccgccgtt cggacgacat ttgatttttg cttctttgac ctgcctcatt     60

gatgcggtat gcaaaaaaag ataccataac caaaatgttt atatattatc tattctgcgt    120

atgactagga gtaaacctgt gaatcgaact gccttctgct gcctttctct gaccactgcc    180

ctgattctga ccgcctgcag cagcggaggg ggtggtgtcg ccgccgacat cggtgcgggg    240

cttgccgatg cactaaccgc accgctcgac cataaagaca aaggtttgca gtctttgacg    300

ctggatcagt ccgtcaggaa aaacgagaaa ctgaagctgg cggcacaagg tgcggaaaaa    360

acttatggaa acggtgacag cctcaatacg ggcaaattga agaacgacaa ggtcagccgt    420

ttcgacttta tccgccaaat cgaagtggac gggcagctca ttaccttgga gagtggagag    480

ttccaagtat acaaacaaag ccattccgcc ttaaccgcct ttcagaccga gcaaatacaa    540

gattcggagc attccgggaa gatggttgcg aaacgccagt tcagaatcgg cgacatagcg    600

ggcgaacata catcttttga caagcttccc gaaggcggca gggcgacata tcgcgggacg    660

gcgttcggtt cagacgatgc cggcggaaaa ctgacctaca ccatagattt cgccgccaag    720

cagggaaacg gcaaaatcga acatttgaaa tcgccagaac tcaatgtcga cctggccgcc    780

gccgatatca agccggatgg aaaacgccat gccgtcatca gcggttccgt cctttacaac    840

caagccgaga aaggcagtta ctccctcggt atctttggcg gaaaagccca ggaagttgcc    900

ggcagcgcgg aagtgaaaac cgtaaacggc atacgccata tcggccttgc cgccaagcaa    960

taa                                                                  963


<210>  7
<211>  1089
<212>  DNA
<213>  N. meningitidis

<400>  7
atgaaacact ttccatccaa agtactgacc acagccatcc ttgccacttt ctgtagcggc     60

gcactggcag ccacaagcga cgacgatgtt aaaaaagctg ccactgtggc cattgttgct    120

gcctacaaca atggccaaga aatcaacggt ttcaaagctg gagagaccat ctacgacatt    180

ggtgaagacg gcacaattac ccaaaaagac gcaactgcag ccgatgttga agccgacgac    240

tttaaaggtc tgggtctgaa aaaagtcgtg actaacctga ccaaaaccgt caatgaaaac    300

aaacaaaacg tcgatgccaa agtaaaagct gcagaatctg aaatagaaaa gttaacaacc    360

aagttagcag acactgatgc cgctttagca gatactgatg ccgctctgga tgaaaccacc    420

aacgccttga ataaattggg agaaaatata acgacatttg ctgaagagac taagacaaat    480

atcgtaaaaa ttgatgaaaa attagaagcc gtggctgata ccgtcgacaa gcatgccgaa    540

gcattcaacg atatcgccga ttcattggat gaaaccaaca ctaaggcaga cgaagccgtc    600

aaaaccgcca atgaagccaa acagacggcc gaagaaacca aacaaaacgt cgatgccaaa    660

gtaaaagctg cagaaactgc agcaggcaaa gccgaagctg ccgctggcac agctaatact    720

gcagccgaca aggccgaagc tgtcgctgca aaagttaccg acatcaaagc tgatatcgct    780

acgaacaaag ctgatattgc taaaaactca gcacgcatcg acagcttgga caaaaacgta    840

gctaatctgc gcaaagaaac ccgccaaggc cttgcagaac aagccgcgct ctccggcctg    900

ttccaacctt acaacgtggg tcggttcaat gtaacggctg cagtcggcgg ctacaaatcc    960

gaatcggcag tcgccatcgg taccggcttc cgctttaccg aaaactttgc cgccaaagca   1020

ggcgtggcag tcggcacttc gtccggttct tccgcagcct accatgtcgg cgtcaattac   1080

gagtggtaa                                                           1089


<210>  8
<211>  561
<212>  DNA
<213>  N. meningitidis

<400>  8
atgaatatga aaaccttatt agcactagcg gttagtgcag tatgttcagt tggtgttgcg     60

caagcacacg agcataatac gatacctaaa ggtgcttcta ttgaagtgaa agtgcaacaa    120

cttgatccag taaacggtaa caaagatgtg ggtacagtga ctattactga atctaactat    180

ggtcttgtgt ttacccctga tttacaagga ttaagcgaag gcttacatgg tttccacatc    240

catgaaaacc caagctgtga gccaaaagaa aaagaaggta aattgacagc tggtttaggc    300

gcaggcggtc actgggatcc taaaggtgca aaacaacatg gttacccatg gcaagatgat    360

gcacacttag gtgatttacc tgcattaact gtattgcatg atggcacagc aacaaatcct    420

gttttagcac cacgtcttaa acatttagat gatgttcgcg gtcactctat tatgatccac    480

acgggtggtg ataatcactc cgatcatcca gctccacttg gcggtggcgg cccacgtatg    540

gcatgtggcg tgattaaata a                                              561


<210>  9
<211>  525
<212>  DNA
<213>  N. meningitidis

<400>  9
atgaaaaaag cacttgccac actgattgcc ctcgctctcc cggccgccgc actggcggaa     60

ggcgcatccg gcttttacgt ccaagccgat gccgcacacg caaaagcctc aagctcttta    120

ggttctgcca aaggcttcag cccgcgcatc tccgcaggct accgcatcaa cgacctccgc    180

ttcgccgtcg attacacgcg ctacaaaaac tataaagccc catccaccga tttcaaactt    240

tacagcatcg gcgcgtccgc catttacgac ttcgacaccc aatcgcccgt caaaccgtat    300

ctcggcgcgc gcttgagcct caaccgcgcc tccgtcgact tgggcggcag cgacagcttc    360

agccaaacct ccatcggcct cggcgtattg acgggcgtaa gctatgccgt taccccgaat    420

gtcgatttgg atgccggcta ccgctacaac tacatcggca aagtcaacac tgtcaaaaac    480

gtccgttccg gcgaactgtc cgccggtgtg cgcgtcaaat tctga                    525


<210>  10
<211>  1179
<212>  DNA
<213>  N. meningitidis

<400>  10
atgcgaaaaa aacttaccgc cctcgtattg tccgcactgc cgcttgcggc cgttgccgat     60

gtcagcctat acggcgaaat caaagccggc gtggaaggca ggaactacca gctgcaattg    120

actgaagcac aagccgctaa cggtggagcg agcggtcagg taaaagttac taaagttact    180

aaggccaaaa gccgcatcag gacgaaaatc agtgatttcg gctcgtttat cggctttaag    240

gggagtgagg atttgggcga cgggctgaag gctgtttggc agcttgagca agacgtatcc    300

gttgccggcg gcggcgcgac ccagtggggc aacagggaat cctttatcgg cttggcaggc    360

gaattcggta cgctgcgcgc cggtcgcgtt gcgaatcagt ttgacgatgc cagccaagcc    420

attgatcctt gggacagcaa taatgatgtg gcttcgcaat tgggtatttt caaacgccac    480

gacgacatgc cggtttccgt acgctacgat tcccccgaat tttccggttt cagcggcagc    540

gttcaattcg ttccgatcca aaacagcaag tccgcctata cgccggctta ttatactaag    600

aatacaaaca ataatcttac tctcgttccg gctgttgtcg gcaagcccgg atcggatgtg    660

tattatgccg gtctgaatta caaaaatggc ggttttgccg ggaactatgc ctttaaatat    720

gcgagacacg ccaatgtcgg acgtaatgct tttgagttgt tcttgatcgg cagcgggagt    780

gatcaagcca aaggtaccga tcccttgaaa aaccatcagg tacaccgtct gacgggcggc    840

tatgaggaag gcggcttgaa tctcgccttg gcggctcagt tggatttgtc tgaaaatggc    900

gacaaaacca aaaacagtac gaccgaaatt gccgccactg cttcctaccg cttcggtaat    960

gcagttccac gcatcagcta tgcccatggt ttcgacttta tcgaacgcgg taaaaaaggc   1020

gaaaatacca gctacgatca aatcatcgcc ggcgttgatt atgatttttc caaacgcact   1080

tccgccatcg tgtctggcgc ttggctgaaa cgcaataccg gcatcggcaa ctacactcaa   1140

attaatgccg cctccgtcgg tttgcgccac aaattctaa                          1179


<210>  11
<211>  996
<212>  DNA
<213>  N. meningitidis

<400>  11
atgaaaaaat ccctgattgc cctgactttg gcagcccttc ctgttgcagc aatggctgac     60

gttaccctgt acggcaccat caaagccggc gtagaaactt cccgctctgt atttcaccag    120

aacggccaag ttactgaagt tacaaccgct accggcatcg ttgatttggg ttcgaaaatc    180

ggcttcaaag gccaagaaga cctcggtaac ggcctgaaag ccatttggca ggttgagcaa    240

aaagcatcta tcgccggtac tgactccggt tggggcaacc gccaatcctt catcggcttg    300

aaaggcggct tcggtaaatt gcgcgtcggt cgtttgaaca gcgtcctgaa agacaccggc    360

gacatcaatc cttgggatag caaaagcgac tatttgggtg taaacaaaat tgccgaaccc    420

gaggcacgcc tcatttccgt acgctacgat tctcccgaat ttgccggcct cagcggcagc    480

gtacaatacg cgcttaacga caatgcaggc agacataaca gcgaatctta ccacgccggc    540

ttcaactaca aaaacggtgg cttcttcgtg caatatggcg gtgcctataa aagacatcat    600

caagtgcaag agggcttgaa tattgagaaa taccagattc accgtttggt cagcggttac    660

gacaatgatg ccctgtacgc ttccgtagcc gtacagcaac aagacgcgaa actgactgat    720

gcttccaatt cgcacaactc tcaaaccgaa gttgccgcta ccttggcata ccgcttcggc    780

aacgtaacgc cccgagtttc ttacgcccac ggcttcaaag gtttggttga tgatgcagac    840

ataggcaacg aatacgacca agtggttgtc ggtgcggaat acgacttctc caaacgcact    900

tctgccttgg tttctgccgg ttggttgcaa gaaggcaaag gcgaaaacaa attcgtagcg    960

actgccggcg gtgtcggtct gcgccacaaa ttctaa                              996


<210>  12
<211>  10
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Neisserial uptake sequence

<400>  12
gccgtctgaa                                                            10


