                         SEQUENCE LISTING

<110>  CENTRE HOSPITALIER UNIVERSITAIRE VAUDOIS (CHUV)
 
<120>  Pichia pastoris deficient in endogenous secreted protease

<130>  17842/PCT

<150>  PCT/IB2009/055729
<151>  2009-12-14

<160>  6     

<170>  PatentIn version 3.3

<210>  1
<211>  1434
<212>  DNA
<213>  Pichia pastoris

<400>  1
atgaaaatta tcagattagc atcagtggct accgtggcta ccctggcaca ctcgcttttg     60

ataccatctc ttgaccaatt gaatatccaa ttgccgtttt ccttgcccca tcatactgag    120

tctccatcgc tgaaattaca gggatctaac ccatttgaat cttccacagt gagacccgac    180

cctatccaga tatattcgac tggatataag gtgattgaga actcctatat tgtcactgta    240

gacagttcca tcactgattc ggaacttcag cagctttatg actacattaa aggtggatac    300

gaattcatgt tgaacaatga agaccccttt ttcgttgcca tgggtattaa gagactaccc    360

aagcattctt tgtttgaacc tttcagtatc ggtgactcca tgaaaggctt tgtcggtttc    420

tttccaccaa gactagttga aaaactggcc tctatggacc ttcctatagt ggctatcgag    480

gcagattcag tagtccattc gacaaaagag tacgcagttg acgaacatgt aacctggggt    540

ttggctagaa tttcccagag gcaaacacta gataccaata gaaattatat ataccatgtc    600

gatggtggca aaaatgttac cgcctatgtc atcgacactg gtatttttgt tgaccatgaa    660

cagtttgaaa agcgggctcg ttggggtgca actatcccat ttggggacgt tgacaaggat    720

gccaatggac acggaactca tgtggctggt acaatcggtt ccgcaaaata tggtgtggct    780

aaacagactt cactggtggc agtaaaggtg ctcaactctg atgggactgg caccctttca    840

gatgtcatta aaggcattga gtttgtggtc aaagaccaca agagctcaag gggcaagtct    900

aagggctctg ttgctaacat gtccttaggt ggtggtctgt cagttgctct cacatacgct    960

gtaaatgctg ctgttgataa tggcatccac tttgccgtag ccgctggaaa tgacaatgct   1020

aacgcttgca gttactctcc agcaaattca gctaaggcaa tcactgcagc tgcctctact   1080

gtcgaggacg aacgtgcgtt cttcagtaac tggggtacct gtgtggacgt ctttgcccca   1140

gggtatctta ttgagtccac ctacattggc cttccaacct ccacaagagt tctttcgggt   1200

acttcgatgg cctctccaca cgttgccggt cttttgagtt actacctctc tctacaaccc   1260

gggcatgaat cgcaatataa ctccggtgac tcattgaccc cagctcaact aaaagctagg   1320

attttgagtt tcagtaccaa ggatgtactt gatgactcgg atctaaacta tggcactcct   1380

aacttgctaa tctacaactc aagagacaac attactgaat tttgggatta ttaa         1434


<210>  2
<211>  477
<212>  PRT
<213>  Pichia pastoris

<400>  2

Met Lys Ile Ile Arg Leu Ala Ser Val Ala Thr Val Ala Thr Leu Ala 
1               5                   10                  15      


His Ser Leu Leu Ile Pro Ser Leu Asp Gln Leu Asn Ile Gln Leu Pro 
            20                  25                  30          


Phe Ser Leu Pro His His Thr Glu Ser Pro Ser Leu Lys Leu Gln Gly 
        35                  40                  45              


Ser Asn Pro Phe Glu Ser Ser Thr Val Arg Pro Asp Pro Ile Gln Ile 
    50                  55                  60                  


Tyr Ser Thr Gly Tyr Lys Val Ile Glu Asn Ser Tyr Ile Val Thr Val 
65                  70                  75                  80  


Asp Ser Ser Ile Thr Asp Ser Glu Leu Gln Gln Leu Tyr Asp Tyr Ile 
                85                  90                  95      


Lys Gly Gly Tyr Glu Phe Met Leu Asn Asn Glu Asp Pro Phe Phe Val 
            100                 105                 110         


Ala Met Gly Ile Lys Arg Leu Pro Lys His Ser Leu Phe Glu Pro Phe 
        115                 120                 125             


Ser Ile Gly Asp Ser Met Lys Gly Phe Val Gly Phe Phe Pro Pro Arg 
    130                 135                 140                 


Leu Val Glu Lys Leu Ala Ser Met Asp Leu Pro Ile Val Ala Ile Glu 
145                 150                 155                 160 


Ala Asp Ser Val Val His Ser Thr Lys Glu Tyr Ala Val Asp Glu His 
                165                 170                 175     


Val Thr Trp Gly Leu Ala Arg Ile Ser Gln Arg Gln Thr Leu Asp Thr 
            180                 185                 190         


Asn Arg Asn Tyr Ile Tyr His Val Asp Gly Gly Lys Asn Val Thr Ala 
        195                 200                 205             


Tyr Val Ile Asp Thr Gly Ile Phe Val Asp His Glu Gln Phe Glu Lys 
    210                 215                 220                 


Arg Ala Arg Trp Gly Ala Thr Ile Pro Phe Gly Asp Val Asp Lys Asp 
225                 230                 235                 240 


Ala Asn Gly His Gly Thr His Val Ala Gly Thr Ile Gly Ser Ala Lys 
                245                 250                 255     


Tyr Gly Val Ala Lys Gln Thr Ser Leu Val Ala Val Lys Val Leu Asn 
            260                 265                 270         


Ser Asp Gly Thr Gly Thr Leu Ser Asp Val Ile Lys Gly Ile Glu Phe 
        275                 280                 285             


Val Val Lys Asp His Lys Ser Ser Arg Gly Lys Ser Lys Gly Ser Val 
    290                 295                 300                 


Ala Asn Met Ser Leu Gly Gly Gly Leu Ser Val Ala Leu Thr Tyr Ala 
305                 310                 315                 320 


Val Asn Ala Ala Val Asp Asn Gly Ile His Phe Ala Val Ala Ala Gly 
                325                 330                 335     


Asn Asp Asn Ala Asn Ala Cys Ser Tyr Ser Pro Ala Asn Ser Ala Lys 
            340                 345                 350         


Ala Ile Thr Ala Ala Ala Ser Thr Val Glu Asp Glu Arg Ala Phe Phe 
        355                 360                 365             


Ser Asn Trp Gly Thr Cys Val Asp Val Phe Ala Pro Gly Tyr Leu Ile 
    370                 375                 380                 


Glu Ser Thr Tyr Ile Gly Leu Pro Thr Ser Thr Arg Val Leu Ser Gly 
385                 390                 395                 400 


Thr Ser Met Ala Ser Pro His Val Ala Gly Leu Leu Ser Tyr Tyr Leu 
                405                 410                 415     


Ser Leu Gln Pro Gly His Glu Ser Gln Tyr Asn Ser Gly Asp Ser Leu 
            420                 425                 430         


Thr Pro Ala Gln Leu Lys Ala Arg Ile Leu Ser Phe Ser Thr Lys Asp 
        435                 440                 445             


Val Leu Asp Asp Ser Asp Leu Asn Tyr Gly Thr Pro Asn Leu Leu Ile 
    450                 455                 460                 


Tyr Asn Ser Arg Asp Asn Ile Thr Glu Phe Trp Asp Tyr 
465                 470                 475         


<210>  3
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic sequence

<400>  3
gttttcgaag aaatgaaaat tatcagatta gcatca                               36


<210>  4
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic sequence

<400>  4
cttctcgagt taataatccc aaaattcagt                                      30


<210>  5
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic sequence


<220>
<221>  n
<222>  (9)..(9)
<223>  inosine

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is a, c, g, or t

<220>
<221>  n
<222>  (12)..(12)
<223>  inosine

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is a, c, g, or t

<220>
<221>  n
<222>  (18)..(18)
<223>  inosine

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or t

<220>
<221>  n
<222>  (21)..(21)
<223>  inosine

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n is a, c, g, or t

<220>
<221>  n
<222>  (24)..(24)
<223>  inosine

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n is a, c, g, or t

<400>  5
ggycacggna cncacgtngc nggnac                                          26


<210>  6
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic sequence


<220>
<221>  n
<222>  (7)..(7)
<223>  inosine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or t

<220>
<221>  n
<222>  (10)..(10)
<223>  inosine

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is a, c, g, or t

<220>
<221>  n
<222>  (16)..(16)
<223>  inosine

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is a, c, g, or t

<220>
<221>  n
<222>  (19)..(19)
<223>  inosine

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  n is a, c, g, or t

<220>
<221>  n
<222>  (22)..(22)
<223>  inosine

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n is a, c, g, or t

<400>  6
gtgrggngtn gccatngang tncc                                            24


