                         SEQUENCE LISTING

<110>  Scil Proteins GmbH
 
<120>  MODIFIED UBIQUITIN PROTEINS HAVING A SPECIFIC BINDING ACTIVITY 
       FOR THE EXTRADOMAIN B OF FIBRONECTIN

<130>  P27761-WO02

<150>  EP09179147.5
<151>  2009-12-14

<150>  EP10186980.8
<151>  2010-10-08

<150>  EP10162264.5
<151>  2010-05-07

<160>  50    

<170>  PatentIn version 3.3

<210>  1
<211>  76
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin protein

<400>  1

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  2
<211>  91
<212>  PRT
<213>  artificial

<220>
<223>  ED-B domain of oncofetal fibronectin

<400>  2

Glu Val Pro Gln Leu Thr Asp Leu Ser Phe Val Asp Ile Thr Asp Ser 
1               5                   10                  15      


Ser Ile Gly Leu Arg Trp Thr Pro Leu Asn Ser Ser Thr Ile Ile Gly 
            20                  25                  30          


Tyr Arg Ile Thr Val Val Ala Ala Gly Glu Gly Ile Pro Ile Phe Glu 
        35                  40                  45              


Asp Phe Val Asp Ser Ser Val Gly Tyr Tyr Thr Val Thr Gly Leu Glu 
    50                  55                  60                  


Pro Gly Ile Asp Tyr Asp Ile Ser Val Ile Thr Leu Ile Asn Gly Gly 
65                  70                  75                  80  


Glu Ser Ala Pro Thr Thr Leu Thr Gln Gln Thr 
                85                  90      


<210>  3
<211>  76
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin variant 1H4

<400>  3

Met Trp Ile Lys Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Thr Leu Ser 
    50                  55                  60                  


Arg Ser Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  4
<211>  76
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin variant 4B10

<400>  4

Met Leu Ile Leu Val Leu Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Ala Thr Lys 
    50                  55                  60                  


Pro Ile Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  5
<211>  76
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin variant 5E1

<400>  5

Met Val Ile Asn Val Phe Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Arg Ser Thr 
    50                  55                  60                  


Ser Lys Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  6
<211>  164
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin dimer 46H9

<400>  6

Met Gly Ile Val Val Arg Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Pro His Pro 
    50                  55                  60                  


Thr Leu Leu His Leu Val Leu Arg Leu Arg Gly Gly Ser Gly Gly Gly 
65                  70                  75                  80  


Gly Ser Gly Gly Gly Gly Ile Gly Met Gln Ile Phe Val His Thr Met 
                85                  90                  95      


Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu 
            100                 105                 110         


Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln 
        115                 120                 125             


Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu 
    130                 135                 140                 


Ser Asp Tyr Asn Ile Lys Pro Ile Ala Glu Leu His Leu Val Leu Arg 
145                 150                 155                 160 


Leu Arg Gly Gly 
                


<210>  7
<211>  164
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin dimer 9E12

<400>  7

Met Arg Ile Pro Val Tyr Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Pro Pro Phe 
    50                  55                  60                  


Ala Arg Leu His Leu Val Leu Arg Leu Arg Gly Gly Ser Gly Gly Gly 
65                  70                  75                  80  


Gly Ser Gly Gly Gly Gly Ile Gly Met Gln Ile Phe Val Met Thr Arg 
                85                  90                  95      


Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu 
            100                 105                 110         


Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln 
        115                 120                 125             


Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu 
    130                 135                 140                 


Ser Asp Tyr Asn Ile Met Asn Ala Arg Leu Leu His Leu Val Leu Arg 
145                 150                 155                 160 


Leu Arg Gly Gly 
                


<210>  8
<211>  164
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin dimer 22D1

<400>  8

Met Leu Ile Leu Val Arg Thr Leu Thr Asp Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Gly Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Ser Val Gly 
    50                  55                  60                  


Ala Met Leu His Leu Val Leu Arg Leu Arg Gly Gly Ser Gly Gly Gly 
65                  70                  75                  80  


Gly Ser Gly Gly Gly Gly Ile Gly Met Gln Ile Phe Val Leu Thr Trp 
                85                  90                  95      


Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu 
            100                 105                 110         


Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln 
        115                 120                 125             


Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu 
    130                 135                 140                 


Ser Asp Tyr Asn Ile Arg Arg Leu Pro Pro Leu His Leu Val Leu Arg 
145                 150                 155                 160 


Leu Arg Gly Gly 
                


<210>  9
<211>  65
<212>  DNA
<213>  artificial

<220>
<223>  Primer F1

<400>  9
ggagaccaca acggtttccc tctagaaata attttgttta actttaagaa ggagatatac     60

atatg                                                                 65


<210>  10
<211>  39
<212>  DNA
<213>  artificial

<220>
<223>  Primer WUBI(co)RD_xho

<400>  10
aaaaaaaaac tcgagaccgc cacgcagacg cagaaccag                            39


<210>  11
<211>  834
<212>  DNA
<213>  artificial

<220>
<223>  Construct His6-SUMO-TNFa

<400>  11
atgggcagca gccatcatca tcatcatcac ggcagcggcc tggtgccgcg cggcagcgct     60

agcatgtcgg actcagaagt caatcaagaa gctaagccag aggtcaagcc agaagtcaag    120

cctgagactc acatcaattt aaaggtgtcc gatggatctt cagagatctt cttcaagatc    180

aaaaagacca ctcctttaag aaggctgatg gaagcgttcg ctaaaagaca gggtaaggaa    240

atggactcct taagattctt gtacgacggt attagaattc aagctgatca gacccctgaa    300

gatttggaca tggaggataa cgatattatt gaggctcaca gagaacagat tggtggtgtg    360

cgtagcagca gccgtacccc gagcgataaa ccggtggcgc atgtggtggc gaatccgcag    420

gcggaaggcc agctgcagtg gctgaaccgt cgtgcgaatg cgctgctggc caacggcgtg    480

gaactgcgtg ataatcagct ggttgtgccg agcgaaggcc tgtatctgat ttatagccag    540

gtgctgttta aaggccaggg ctgcccgagc acccatgtgc tgctgaccca taccattagc    600

cgtattgcgg tgagctatca gaccaaagtg aacctgctgt ctgcgattaa aagcccgtgc    660

cagcgtgaaa ccccggaagg cgcggaagcg aaaccgtggt atgaaccgat ttatctgggc    720

ggcgtgtttc agctggaaaa aggcgatcgt ctgagcgcgg aaattaaccg tccggattat    780

ctggattttg cggaaagcgg ccaggtgtat tttggcatta ttgcgctgta ataa          834


<210>  12
<211>  27
<212>  DNA
<213>  artificial

<220>
<223>  Primer SUMO-EDB-TNFa-fw

<400>  12
ttttttggat ccgtgcgtag cagcagc                                         27


<210>  13
<211>  28
<212>  DNA
<213>  artificial

<220>
<223>  Primer SUMO-EDB-TNFa-rev

<400>  13
cttgtctctc gaggcggccg cttattac                                        28


<210>  14
<211>  33
<212>  DNA
<213>  artificial

<220>
<223>  Primer SUMO-EDB-WUBI-fw

<400>  14
gttccaaggt ctcatggtat gcagatcttc gtg                                  33


<210>  15
<211>  42
<212>  DNA
<213>  artificial

<220>
<223>  Primer SUMO-EDB-Linker-rev

<400>  15
gtggtgggat ccaccgccac caccagaacc gccacgcaga cg                        42


<210>  16
<211>  33
<212>  DNA
<213>  artificial

<220>
<223>  Primer SUMO-EDB-1H4-fw

<400>  16
gttccaaggt ctcatggtat gtggatcaag gtg                                  33


<210>  17
<211>  33
<212>  DNA
<213>  artificial

<220>
<223>  Primer SUMO-EDB-4B10-fw

<400>  17
gttccaaggt ctcatggtat gttgatcctg gtg                                  33


<210>  18
<211>  33
<212>  DNA
<213>  artificial

<220>
<223>  Primer SUMO-EDB-5E1-fw

<400>  18
gttccaaggt ctcatggtat ggttatcaat gtg                                  33


<210>  19
<211>  42
<212>  DNA
<213>  artificial

<220>
<223>  Primer Dimer-t0a-rev

<400>  19
gtggtgggat ccaccgccac caccagaacc accacgtaaa cg                        42


<210>  20
<211>  33
<212>  DNA
<213>  artificial

<220>
<223>  Primer SUMO-EDB-WUBI-fw

<400>  20
gttccaaggt ctcatggtat gcagatcttc gtg                                  33


<210>  21
<211>  33
<212>  DNA
<213>  artificial

<220>
<223>  Primer 9E12-t0a-fw

<400>  21
gttccaaggt ctcatggtat gcgtatccct gtg                                  33


<210>  22
<211>  33
<212>  DNA
<213>  artificial

<220>
<223>  Primer 24H12-t0a-fw

<400>  22
gttccaaggt ctcatggtat ggttatcaag gtg                                  33


<210>  23
<211>  33
<212>  DNA
<213>  artificial

<220>
<223>  Primer 15G7-t0a-fw

<400>  23
gttccaaggt ctcatggtat ggagatcggt gtg                                  33


<210>  24
<211>  33
<212>  DNA
<213>  artificial

<220>
<223>  Primer 22D1-t0a-fw

<400>  24
gttccaaggt ctcatggtat gcttatcttg gtg                                  33


<210>  25
<211>  164
<212>  PRT
<213>  artificial

<220>
<223>  Variant 46-H4

<400>  25

Met Gly Ile Val Val Arg Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Pro His Pro 
    50                  55                  60                  


Thr Leu Leu His Leu Val Leu Arg Leu Arg Gly Gly Ser Gly Gly Gly 
65                  70                  75                  80  


Gly Ser Gly Gly Gly Gly Ile Gly Met Gln Ile Phe Val Gly Thr Trp 
                85                  90                  95      


Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu 
            100                 105                 110         


Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln 
        115                 120                 125             


Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu 
    130                 135                 140                 


Ser Asp Tyr Asn Ile Thr Gln Ala Thr Arg Leu His Leu Val Leu Arg 
145                 150                 155                 160 


Leu Arg Gly Gly 
                


<210>  26
<211>  164
<212>  PRT
<213>  artificial

<220>
<223>  Variant 45-H9

<400>  26

Met Arg Ile Pro Val Tyr Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Pro Pro Phe 
    50                  55                  60                  


Ala Arg Leu His Leu Val Leu Arg Leu Arg Gly Gly Ser Gly Gly Gly 
65                  70                  75                  80  


Gly Ser Gly Gly Gly Gly Ile Gly Met Gln Ile Phe Val Leu Thr Met 
                85                  90                  95      


Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu 
            100                 105                 110         


Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln 
        115                 120                 125             


Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu 
    130                 135                 140                 


Ser Asp Tyr Asn Ile Leu Ala Phe Ala Thr Leu His Leu Val Leu Arg 
145                 150                 155                 160 


Leu Arg Gly Gly 
                


<210>  27
<211>  164
<212>  PRT
<213>  artificial

<220>
<223>  Variant 46-A5

<400>  27

Met Gly Ile Val Val Arg Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Pro His Pro 
    50                  55                  60                  


Thr Leu Leu His Leu Val Leu Arg Leu Arg Gly Gly Ser Gly Gly Gly 
65                  70                  75                  80  


Gly Ser Gly Gly Gly Gly Ile Gly Met Gln Ile Phe Val Leu Thr Met 
                85                  90                  95      


Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu 
            100                 105                 110         


Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln 
        115                 120                 125             


Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu 
    130                 135                 140                 


Ser Asp Tyr Asn Ile Leu Ala Phe Ala Thr Leu His Leu Val Leu Arg 
145                 150                 155                 160 


Leu Arg Gly Gly 
                


<210>  28
<211>  164
<212>  PRT
<213>  artificial

<220>
<223>  Variant 50-G11

<400>  28

Met Gly Ile Val Val Arg Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Pro His Pro 
    50                  55                  60                  


Thr Leu Leu His Leu Val Leu Arg Leu Arg Gly Gly Ser Gly Gly Gly 
65                  70                  75                  80  


Gly Ser Gly Gly Gly Gly Ile Gly Met Gln Ile Phe Val Met Thr Arg 
                85                  90                  95      


Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu 
            100                 105                 110         


Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln 
        115                 120                 125             


Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu 
    130                 135                 140                 


Ser Asp Tyr Asn Ile Met Asn Ala Arg Leu Leu His Leu Val Leu Arg 
145                 150                 155                 160 


Leu Arg Gly Gly 
                


<210>  29
<211>  164
<212>  PRT
<213>  artificial

<220>
<223>  Variant 52-B3

<400>  29

Met Arg Ile Pro Val Tyr Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Pro Pro Phe 
    50                  55                  60                  


Ala Arg Leu His Leu Val Leu Arg Leu Arg Gly Gly Ser Gly Gly Gly 
65                  70                  75                  80  


Gly Ser Gly Gly Gly Gly Ile Gly Met Gln Ile Phe Val His Thr Met 
                85                  90                  95      


Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu 
            100                 105                 110         


Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln 
        115                 120                 125             


Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu 
    130                 135                 140                 


Ser Asp Tyr Asn Ile Lys Pro Ile Ala Glu Leu His Leu Val Leu Arg 
145                 150                 155                 160 


Leu Arg Gly Gly 
                


<210>  30
<211>  164
<212>  PRT
<213>  artificial

<220>
<223>  Variant 52-D10

<400>  30

Met Val Ile Cys Val Arg Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Thr Ala Pro 
    50                  55                  60                  


Gly Asp Leu His Leu Val Leu Arg Leu Arg Gly Gly Ser Gly Gly Gly 
65                  70                  75                  80  


Gly Ser Gly Gly Gly Gly Ile Gly Met Gln Ile Phe Val His Thr Met 
                85                  90                  95      


Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu 
            100                 105                 110         


Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln 
        115                 120                 125             


Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu 
    130                 135                 140                 


Ser Asp Tyr Asn Ile Lys Pro Ile Ala Glu Leu His Leu Val Leu Arg 
145                 150                 155                 160 


Leu Arg Gly Gly 
                


<210>  31
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  His-Tag

<400>  31

Leu Glu His His His His His His 
1               5               


<210>  32
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  Glycine/serine linker

<400>  32

Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ile Gly 
1               5                   10          


<210>  33
<211>  155
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin dimer SPVF-28_1041-D11_TsX9

<400>  33

Met Gln Ile Phe Val Trp Thr Trp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Arg Lys 
    50                  55                  60                  


Phe Pro Leu His Leu Val Leu Arg Leu Arg Gly Gly Gly Ile Gly Met 
65                  70                  75                  80  


Arg Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Trp Ser Asn Trp 
    130                 135                 140                 


Glu Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 155 


<210>  34
<211>  155
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin dimer SPVF-28_1045-D10_TsX9

<400>  34

Met Gln Ile Phe Val Trp Thr Trp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Arg Lys 
    50                  55                  60                  


Phe Pro Leu His Leu Val Leu Arg Leu Arg Gly Gly Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Trp Ser Asn Trp 
    130                 135                 140                 


Glu Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 155 


<210>  35
<211>  319
<212>  PRT
<213>  artificial

<220>
<223>  fusion protein SPVF-28_1041-D11_T0aX9

<400>  35

Met Gln Ile Phe Val Trp Thr Trp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Arg Lys 
    50                  55                  60                  


Phe Pro Leu His Leu Val Leu Arg Leu Arg Gly Gly Gly Ile Gly Met 
65                  70                  75                  80  


Arg Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Trp Ser Asn Trp 
    130                 135                 140                 


Glu Leu His Leu Val Leu Arg Leu Arg Ala Ala Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala 
                165                 170                 175     


His Val Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn 
            180                 185                 190         


Arg Arg Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn 
        195                 200                 205             


Gln Leu Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val 
    210                 215                 220                 


Leu Phe Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His 
225                 230                 235                 240 


Thr Ile Ser Arg Ile Ala Val Ser Tyr Gln Thr Lys Val Asn Leu Leu 
                245                 250                 255     


Ser Ala Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu 
            260                 265                 270         


Ala Lys Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu 
        275                 280                 285             


Glu Lys Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu 
    290                 295                 300                 


Asp Phe Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
305                 310                 315                 


<210>  36
<211>  318
<212>  PRT
<213>  artificial

<220>
<223>  fusion protein SPVF-28_1041-D11_T0uX9

<400>  36

Met Gln Ile Phe Val Trp Thr Trp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Arg Lys 
    50                  55                  60                  


Phe Pro Leu His Leu Val Leu Arg Leu Arg Gly Gly Gly Ile Gly Met 
65                  70                  75                  80  


Arg Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Trp Ser Asn Trp 
    130                 135                 140                 


Glu Leu His Leu Val Leu Arg Leu Arg Ala Ala Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Leu Arg Ser Ser Ser Gln Asn Ser Ser Asp Lys Pro Val Ala 
                165                 170                 175     


His Val Val Ala Asn His Gln Val Glu Glu Gln Leu Glu Trp Leu Ser 
            180                 185                 190         


Gln Arg Ala Asn Ala Leu Leu Ala Asn Gly Met Asp Leu Lys Asp Asn 
        195                 200                 205             


Gln Leu Val Val Pro Ala Asp Gly Leu Tyr Leu Val Tyr Ser Gln Val 
    210                 215                 220                 


Leu Phe Lys Gly Gln Gly Cys Pro Asp Tyr Val Leu Leu Thr His Thr 
225                 230                 235                 240 


Val Ser Arg Phe Ala Ile Ser Tyr Gln Glu Lys Val Asn Leu Leu Ser 
                245                 250                 255     


Ala Val Lys Ser Pro Cys Pro Lys Asp Thr Pro Glu Gly Ala Glu Leu 
            260                 265                 270         


Lys Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu 
        275                 280                 285             


Lys Gly Asp Gln Leu Ser Ala Glu Val Asn Leu Pro Lys Tyr Leu Asp 
    290                 295                 300                 


Phe Ala Glu Ser Gly Gln Val Tyr Phe Gly Val Ile Ala Leu 
305                 310                 315             


<210>  37
<211>  155
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin dimer SPVF-28_1048-E10_TsX9

<400>  37

Met Gln Ile Phe Val Trp Thr His Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly His Thr Leu Ser Asp Tyr Asn Ile Pro Arg Arg 
    50                  55                  60                  


Ser Trp Leu His Leu Val Leu Arg Leu Arg Gly Gly Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val Ser Thr Thr Thr Gly Glu Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Ala Asp Pro Arg 
    130                 135                 140                 


Trp Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 155 


<210>  38
<211>  155
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin dimer SPVF-28_1041-E6_TsX9

<400>  38

Met Gln Ile Phe Val Trp Thr His Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Pro Arg Arg 
    50                  55                  60                  


Ser Trp Leu His Leu Val Leu Arg Leu Arg Gly Gly Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val Ser Thr Thr Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Arg 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Ala Asp Pro Arg 
    130                 135                 140                 


Trp Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 155 


<210>  39
<211>  155
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin dimer SPVF-28_1041-H10_TsX9

<400>  39

Met Gln Ile Phe Val Trp Thr Asn Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Glu His Gly 
    50                  55                  60                  


Lys Trp Leu His Leu Val Leu Arg Leu Arg Gly Gly Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val Asn Thr Thr Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Phe Ile Gly His 
    130                 135                 140                 


Trp Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 155 


<210>  40
<211>  158
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin dimer SPVF-28_1056-B6_TsX6

<400>  40

Met Gln Ile Phe Val His Thr His Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Arg Asp 
    50                  55                  60                  


Lys Arg Leu His Leu Val Leu Arg Leu Arg Ala Ala Ser Gly Gly Gly 
65                  70                  75                  80  


Gly Ile Gly Met Gln Ile Phe Val Asn Thr Asn Thr Gly Glu Thr Ile 
                85                  90                  95      


Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys 
            100                 105                 110         


Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp 
        115                 120                 125             


Ala Gly Lys Arg Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile 
    130                 135                 140                 


Asp Trp Arg Trp Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 155             


<210>  41
<211>  154
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin dimer SPVF-28_1051-B7_TsX9

<400>  41

Met Gln Ile Phe Val His Thr Thr Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Thr Leu Thr 
    50                  55                  60                  


Pro Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val Leu Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Trp Arg Trp 
    130                 135                 140                 


Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 


<210>  42
<211>  155
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin dimer SPVF-28_1035-E6_TsX9

<400>  42

Met Gln Ile Phe Val His Thr Phe Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile His Glu Arg 
    50                  55                  60                  


Glu Ile Leu His Leu Val Leu Arg Leu Arg Gly Gly Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val Ser Thr Pro Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly Val Glu Met 
    130                 135                 140                 


Leu Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 155 


<210>  43
<211>  154
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin dimer SPVF-28_1049-D4_TsX9

<400>  43

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Glu Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile His Asn Trp 
    50                  55                  60                  


Arg Asn Leu His Leu Val Leu Arg Leu Arg Gly Gly Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val Ile Thr Ile Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Lys Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Trp Arg Trp 
    130                 135                 140                 


Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 


<210>  44
<211>  164
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin dimer SPWF-28_1071-C8_TsX2

<400>  44

Met Trp Ile Arg Val Pro Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile His Met Pro 
    50                  55                  60                  


Asp Ile Leu His Leu Val Leu Arg Leu Arg Gly Gly Ser Gly Gly Gly 
65                  70                  75                  80  


Gly Ser Gly Gly Gly Gly Ile Gly Met Gln Ile Phe Val Trp Thr Met 
                85                  90                  95      


Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu 
            100                 105                 110         


Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln 
        115                 120                 125             


Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu 
    130                 135                 140                 


Ser Asp Tyr Asn Ile His Leu His Met Arg Leu His Leu Val Leu Arg 
145                 150                 155                 160 


Leu Arg Gly Gly 
                


<210>  45
<211>  164
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin dimer SPWF-28_1071-C12_TsX2

<400>  45

Met Thr Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Phe Lys 
    50                  55                  60                  


Leu Ser Leu His Leu Val Leu Arg Leu Arg Gly Gly Ser Gly Gly Gly 
65                  70                  75                  80  


Gly Ser Gly Gly Gly Gly Ile Gly Met Gln Ile Phe Val Ser Thr Phe 
                85                  90                  95      


Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu 
            100                 105                 110         


Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln 
        115                 120                 125             


Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu 
    130                 135                 140                 


Ser Asp Tyr Asn Ile His Tyr Leu Pro Lys Leu His Leu Val Leu Arg 
145                 150                 155                 160 


Leu Arg Gly Gly 
                


<210>  46
<211>  172
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin dimer SPWF-28_1071-H7_TsX2

<400>  46

Met Trp Ile Arg Val Pro Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Arg Arg Val 
    50                  55                  60                  


Asn Tyr Leu His Leu Val Leu Arg Leu Arg Gly Gly Ser Gly Gly Gly 
65                  70                  75                  80  


Gly Ser Gly Gly Gly Gly Ile Gly Met Gln Ile Phe Val Trp Thr Ser 
                85                  90                  95      


Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu 
            100                 105                 110         


Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln 
        115                 120                 125             


Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu 
    130                 135                 140                 


Ser Asp Tyr Asn Ile Tyr Thr Tyr Met Arg Leu His Leu Val Leu Arg 
145                 150                 155                 160 


Leu Arg Gly Gly Leu Glu His His His His His His 
                165                 170         


<210>  47
<211>  164
<212>  PRT
<213>  artificial

<220>
<223>  Ubiquitin dimer with linker SGGGGSGGGGIG


<220>
<221>  misc_feature
<222>  (94)..(94)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (96)..(96)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (150)..(154)
<223>  Xaa can be any naturally occurring amino acid

<400>  47

Met Thr Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Phe Lys 
    50                  55                  60                  


Leu Ser Leu His Leu Val Leu Arg Leu Arg Gly Gly Ser Gly Gly Gly 
65                  70                  75                  80  


Gly Ser Gly Gly Gly Gly Ile Gly Met Gln Ile Phe Val Xaa Thr Xaa 
                85                  90                  95      


Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu 
            100                 105                 110         


Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln 
        115                 120                 125             


Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu 
    130                 135                 140                 


Ser Asp Tyr Asn Ile Xaa Xaa Xaa Xaa Xaa Leu His Leu Val Leu Arg 
145                 150                 155                 160 


Leu Arg Gly Gly 
                


<210>  48
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Glycine/serine linker

<400>  48

Ser Gly Gly Gly Gly 
1               5   


<210>  49
<211>  7
<212>  PRT
<213>  artificial

<220>
<223>  Glycine/serine linker

<400>  49

Ser Gly Gly Gly Gly Ile Gly 
1               5           


<210>  50
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  Glycine/serine linker

<400>  50

Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10  


