                         SEQUENCE LISTING

<110>  The Johns Hopkins University
 
<120>  METHOD FOR LATE INTRODUCTION OF THE (8R)-HYDROXYL GROUP IN 
       CARBAPENEM BETA-LACTAM ANTIBIOTIC SYNTHESIS

<130>  C10954_P10954-02

<150>  61/285,608
<151>  2009-12-11

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  27
<212>  DNA
<213>  Streptomyces cattleya


<220>
<221>  misc_feature
<223>  thnQ forward primer

<400>  1
gccaagcttt cacccgccgc ggaccag                                           27


<210>  2
<211>  30
<212>  DNA
<213>  Streptomyces cattleya


<220>
<221>  misc_feature
<223>  thnQ reverse primer

<400>  2
cactaactcg agcccgccgc ggaccaggtc                                        30


<210>  3
<211>  29
<212>  DNA
<213>  Streptomyces cattleya


<220>
<221>  misc_feature
<223>  thnG forward primer

<400>  3
ctagtctaga ggcctcctcg gcggagaag                                         29


<210>  4
<211>  29
<212>  DNA
<213>  Streptomyces cattleya


<220>
<221>  misc_feature
<223>  thnG reverse primer

<400>  4
ttataagctt tggccatggt gaacggctg                                         29


<210>  5
<211>  780
<212>  DNA
<213>  Streptomyces cattleya


<220>
<221>  misc_feature
<223>  Nucleotide Sequence for thnQ

<400>  5
atgaccgcct cacatgacac ggcagacgcc acggaccgga ccgtcggttc cacgtccggc       60

gaacgagcgg cgttcgcccg tgacggagtg gtgcgttggg gccgcctgct gacgcctgat      120

cagatcgacg cgctgcggtc ctcggtggag cgtgccttct tccgcgacgg ccaccccgcc      180

gacggggtac gcgatctgtc cgaacgccag ggccgcccgc tcgacctggc cctgctgcac      240

aagatcaacc tgtggcggac cgacgaggcg tgcgcggccc aggtggccag ggccgacctg      300

gcggaccggg ccgaggcgct gctcggcggg ccggtacggc tctaccgcga ccacgtcttc      360

tacaagccgc cgggcaaggg cgaccgcagc cgcatggtgc tccaccagga caaccgctac      420

tggcacctgg acccgccgga ggccatcacg gtgtggatgg cgctggacga cgccaccgtg      480

gagaacgggt gcgtccacta cgtcctcggc agccaccgcc acggccgggt ggagcacgtg      540

cgtcccgagg agggcgcggt gatgatcgag gcccgtaccg agcaggagcc ggtggcctac      600

ccggcccccg ccggggacgc cctggtgcac agcgtcaaca cgctgcacgg ctcggggccc      660

aacctcagcg acggaccgcg ccgcgcctac gtggtggtct acgtgcgcga cggggtgacg      720

atgcgcggcg agccgatgac ctcgttcccg ctcgtcggcg acctggtccg cggcgggtga      780


<210>  6
<211>  792
<212>  DNA
<213>  Streptomyces cattleya


<220>
<221>  misc_feature
<223>  Nucleotide Sequence for thnG

<400>  6
tcaccgtccg ctctccggcc cggcggggtg gcggggcagc gaatagcagt aggagacggc       60

acgccggtcg tcctcggtgg tgttgggcgc cgaggtgtgg gcgacgtagc cgttgaacag      120

cagcgccgag ccggccttgc ggggcagggt gaccggggtg aggccgggcc agatgcgtgc      180

gatgtccagg gacggttcgc gcggtgaggc gaagatgtcg ccggggtcga tgccgtccgg      240

ttcgtagagt ttgcgttccc ggtgggagcc gggcaggaag cgcaggcagc cgttgtcctc      300

gcgggcgtcg tccaccgcga tccagatggt gtagacgtcc acgtacttct cgtgccactc      360

gtcggccagg aagatggcgt cctggtgcca gggtttctgg gagccgaccc gggagggctt      420

ggcccacagg aagcagtcgt agaagtcacc gggcaggccg tgcagcggtt cgatcagctt      480

ctcgttgagg ccgacgaggt cggggacggc ggcgaggtcg gcggagtgtt cgtgggcgcg      540

gccgatgatg cgcaccttgc cgggtatgca ctccttgccg gccgcccggg cggcccatcc      600

gccgccctcc ccctcggcct ccagctgcca ttcctcggtg ctgatgcgca tccgctcggt      660

gcggccgatc agttcggcgc agccgcggcg catcacctcg agtacggatt cgggcaccag      720

gtcgtccagc gccacgacac cgtcgcgccg cagttcgtcg gacagggcct ggatcgactg      780

ggtcaccggc at                                                          792


<210>  7
<211>  259
<212>  PRT
<213>  Streptomyces cattleya


<220>
<221>  MISC_FEATURE
<223>  Amino Acid Sequence for thnQ

<300>
<308>  CAD18985.1
<309>  2005-04-15
<313>  (1)..(259)

<400>  7

Met Thr Ala Ser His Asp Thr Ala Asp Ala Thr Asp Arg Thr Val Gly 
1               5                   10                  15      


Ser Thr Ser Gly Glu Arg Ala Ala Phe Ala Arg Asp Gly Val Val Arg 
            20                  25                  30          


Trp Gly Arg Leu Leu Thr Pro Asp Gln Ile Asp Ala Leu Arg Ser Ser 
        35                  40                  45              


Val Glu Arg Ala Phe Phe Arg Asp Gly His Pro Ala Asp Gly Val Arg 
    50                  55                  60                  


Asp Leu Ser Glu Arg Gln Gly Arg Pro Leu Asp Leu Ala Leu Leu His 
65                  70                  75                  80  


Lys Ile Asn Leu Trp Arg Thr Asp Glu Ala Cys Ala Ala Gln Val Ala 
                85                  90                  95      


Arg Ala Asp Leu Ala Asp Arg Ala Glu Ala Leu Leu Gly Gly Pro Val 
            100                 105                 110         


Arg Leu Tyr Arg Asp His Val Phe Tyr Lys Pro Pro Gly Lys Gly Asp 
        115                 120                 125             


Arg Ser Arg Met Val Leu His Gln Asp Asn Arg Tyr Trp His Leu Asp 
    130                 135                 140                 


Pro Pro Glu Ala Ile Thr Val Trp Met Ala Leu Asp Asp Ala Thr Val 
145                 150                 155                 160 


Glu Asn Gly Cys Val His Tyr Val Leu Gly Ser His Arg His Gly Arg 
                165                 170                 175     


Val Glu His Val Arg Pro Glu Glu Gly Ala Val Met Ile Glu Ala Arg 
            180                 185                 190         


Thr Glu Gln Glu Pro Val Ala Tyr Pro Ala Pro Ala Gly Asp Ala Leu 
        195                 200                 205             


Val His Ser Val Asn Thr Leu His Gly Ser Gly Pro Asn Leu Ser Asp 
    210                 215                 220                 


Gly Pro Arg Arg Ala Tyr Val Val Val Tyr Val Arg Asp Gly Val Thr 
225                 230                 235                 240 


Met Arg Gly Glu Pro Met Thr Ser Phe Pro Leu Val Gly Asp Leu Val 
                245                 250                 255     


Arg Gly Gly 
            


<210>  8
<211>  263
<212>  PRT
<213>  Streptomyces cattleya


<220>
<221>  MISC_FEATURE
<223>  Amino Acid Sequence for thnG

<300>
<308>  CAD18975.1
<309>  2005-04-15
<313>  (1)..(263)

<400>  8

Met Pro Val Thr Gln Ser Ile Gln Ala Leu Ser Asp Glu Leu Arg Arg 
1               5                   10                  15      


Asp Gly Val Val Ala Leu Asp Asp Leu Val Pro Glu Ser Val Leu Glu 
            20                  25                  30          


Val Met Arg Arg Gly Cys Ala Glu Leu Ile Gly Arg Thr Glu Arg Met 
        35                  40                  45              


Arg Ile Ser Thr Glu Glu Trp Gln Leu Glu Ala Glu Gly Glu Gly Gly 
    50                  55                  60                  


Gly Trp Ala Ala Arg Ala Ala Gly Lys Glu Cys Ile Pro Gly Lys Val 
65                  70                  75                  80  


Arg Ile Ile Gly Arg Ala His Glu His Ser Ala Asp Leu Ala Ala Val 
                85                  90                  95      


Pro Asp Leu Val Gly Leu Asn Glu Lys Leu Ile Glu Pro Leu His Gly 
            100                 105                 110         


Leu Pro Gly Asp Phe Tyr Asp Cys Phe Leu Trp Ala Lys Pro Ser Arg 
        115                 120                 125             


Val Gly Ser Gln Lys Pro Trp His Gln Asp Ala Ile Phe Leu Ala Asp 
    130                 135                 140                 


Glu Trp His Glu Lys Tyr Val Asp Val Tyr Thr Ile Trp Ile Ala Val 
145                 150                 155                 160 


Asp Asp Ala Arg Glu Asp Asn Gly Cys Leu Arg Phe Leu Pro Gly Ser 
                165                 170                 175     


His Arg Glu Arg Lys Leu Tyr Glu Pro Asp Gly Ile Asp Pro Gly Asp 
            180                 185                 190         


Ile Phe Ala Ser Pro Arg Glu Pro Ser Leu Asp Ile Ala Arg Ile Trp 
        195                 200                 205             


Pro Gly Leu Thr Pro Val Thr Leu Pro Arg Lys Ala Gly Ser Ala Leu 
    210                 215                 220                 


Leu Phe Asn Gly Tyr Val Ala His Thr Ser Ala Pro Asn Thr Thr Glu 
225                 230                 235                 240 


Asp Asp Arg Arg Ala Val Ser Tyr Cys Tyr Ser Leu Pro Arg His Pro 
                245                 250                 255     


Ala Gly Pro Glu Ser Gly Arg 
            260             


