
                                SEQUENCE LISTING

<110> Indiana University Research and Technology Corporation
      Christopher Burlak
      A. Joseph Tector

<120> Methods of Modulating Thrombocytopenia
  and Modified Transgenic Pigs
  

<130> 12972-00424 W

<150> 61/265,611          
<151> 2009-12-01  

<160> 13

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer Sequence ASGR1 Forward

<400> 1
gcgagcacag acgccaaggt                                             20

<210> 2
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer Sequence ASGR1 reverse

<400> 2
gtgggcacag tcctcgccac                                             20

<210> 3
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer Sequence CD31 forward

<400> 3
acctggtcac cgtgagggaa                                             20

<210> 4
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer Sequence CD31 reverse

<400> 4
atttccggga acgactgggc                                             20

<210> 5
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer Sequence CD105 forward

<400> 5
accagctctt cctctgggca                                             20

<210> 6
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer Sequence CD105 reverse

<400> 6
catgtccttg cggggttcca                                             20

<210> 7
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer Sequence beta-actin forward

<400> 7
tccctggaga agagctacga                                             20

<210> 8
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer Sequence beta actin reverse

<400> 8
tgttggcgta gaggtccttc                                             20

<210> 9
<211> 1348
<212> DNA
<213> Sus scrofa

<220> 
<221> CDS            
<222> (218)...(1078)
<223> porcine ASGR1

<400> 9
gggcagacgc acagggctca gggcccgcgt atggaccctg ccccctccgc tcccactttc 60
cagggctgtc cgcgcaccct aattctccag ccttcggccg ctctcccagc cgggcatctg 120
cacagcagtg agggacccag gagtccacct tgagaccttc agcaacctca gccttaacct 180
tggcggtgac ctggttcgag gtctagccag ccttagc atg aca aag gaa tat cag  235
                                         Met Thr Lys Glu Tyr Gln
                                          1               5
 
gat ctg cag cat ctg gac aat gag gag aat gac cag cag cac aga aaa   283
Asp Leu Gln His Leu Asp Asn Glu Glu Asn Asp Gln Gln His Arg Lys
             10                  15                  20
 
ggg cca cct cct caa ccg tca ctc ctt cgg cgt ctc tgc tcg gga ccc   331
Gly Pro Pro Pro Gln Pro Ser Leu Leu Arg Arg Leu Cys Ser Gly Pro
         25                  30                  35
 
tgc ctc ctc ctg att tcc atg ggc ctt agc ctc ctg ctg ctg gta gtt   379
Cys Leu Leu Leu Ile Ser Met Gly Leu Ser Leu Leu Leu Leu Val Val
     40                  45                  50
 
gtc tgt gtg atc gga tcc cag aac tcc aag ctg cag gag gag ctg cag   427
Val Cys Val Ile Gly Ser Gln Asn Ser Lys Leu Gln Glu Glu Leu Gln
 55                  60                  65                  70
 
gcc ctg aga gag acc ttc agc aac ctc acc gcg agc aca gac gcc aag   475
Ala Leu Arg Glu Thr Phe Ser Asn Leu Thr Ala Ser Thr Asp Ala Lys
                 75                  80                  85
 
gtc aag acc ctc agc atg cag gga gga aat gtg ggc aga aag atg aag   523
Val Lys Thr Leu Ser Met Gln Gly Gly Asn Val Gly Arg Lys Met Lys
             90                  95                 100
 
tcc ctg gag tcc cag ctg gag aaa cag caa cag gac ctg agt gaa gat   571
Ser Leu Glu Ser Gln Leu Glu Lys Gln Gln Gln Asp Leu Ser Glu Asp
        105                 110                 115
 
cac tcc agc ttg ctg ctc cac gtg aag cag ttt gtg tcc gac ctg cgg   619
His Ser Ser Leu Leu Leu His Val Lys Gln Phe Val Ser Asp Leu Arg
    120                 125                 130
 
agc ctc agc tgt cag atg gct gtc ctc cag ggc aat ggc tct gaa agg   667
Ser Leu Ser Cys Gln Met Ala Val Leu Gln Gly Asn Gly Ser Glu Arg
135                 140                 145                 150
 
acc tgc tgc ccg gtt aac tgg gtg ggc tat gaa ggc agc tgc tac tgg   715
Thr Cys Cys Pro Val Asn Trp Val Gly Tyr Glu Gly Ser Cys Tyr Trp
                155                 160                 165
 
ttt tcc cgc tct ggg aag ccc tgg ccg gag gcc gag aag tac tgc cag   763
Phe Ser Arg Ser Gly Lys Pro Trp Pro Glu Ala Glu Lys Tyr Cys Gln
            170                 175                 180
 
ctg gag aat gcc cac ctc gtg gtg gtg ggc tcc tgg gag gag cag aaa   811
Leu Glu Asn Ala His Leu Val Val Val Gly Ser Trp Glu Glu Gln Lys
        185                 190                 195
 
ttt atc cag cac cac gtg ggc cct gtg aac tcc tgg atc ggc ctc act   859
Phe Ile Gln His His Val Gly Pro Val Asn Ser Trp Ile Gly Leu Thr
    200                 205                 210
 
gat cag agc ggg ccc tgg aag tgg gtg gat ggc acc gac tac gag tcg   907
Asp Gln Ser Gly Pro Trp Lys Trp Val Asp Gly Thr Asp Tyr Glu Ser
215                 220                 225                 230
 
ggc ttc aag aac tgg aga ccc gag cag ccg gat gac tgg tac ggg cat   955
Gly Phe Lys Asn Trp Arg Pro Glu Gln Pro Asp Asp Trp Tyr Gly His
                235                 240                 245
 
ggg ctc ggg ggt ggc gag gac tgt gcc cac ttt acg gag gac ggc ggc   1003
Gly Leu Gly Gly Gly Glu Asp Cys Ala His Phe Thr Glu Asp Gly Gly
            250                 255                 260
 
tgg aac gat gac atc tgc cag agg ccc tac cgc tgg gtc tgc gag aca   1051
Trp Asn Asp Asp Ile Cys Gln Arg Pro Tyr Arg Trp Val Cys Glu Thr
        265                 270                 275
 
cag cgg gac agg gac agc ggc agc tag gagtctcctc tccctctaat         1098
Gln Arg Asp Arg Asp Ser Gly Ser  *
    280                 285
 
ttatgtcctc aatgctttta cctgccacgg gggtcttggt tgggggaccc tcccctctgg 1158
gtgcttccgg attttcacct cggattttga gggaagggag aagggtgggg tctgaggaat 1218
ggagagtgat gtttggaggg gtggggagtt tgaaacgcct gccagtttct gtagtttgca 1278
gggtattatt gtcaactttt tttttttaag agtaaaaaga agtgaaatat acaaaaaaaa 1338
aaaaaaaaaa                                                        1348

<210> 10
<211> 286
<212> PRT
<213> Sus scrofa

<400> 10
Met Thr Lys Glu Tyr Gln Asp Leu Gln His Leu Asp Asn Glu Glu Asn
 1               5                  10                  15      
Asp Gln Gln His Arg Lys Gly Pro Pro Pro Gln Pro Ser Leu Leu Arg
            20                  25                  30          
Arg Leu Cys Ser Gly Pro Cys Leu Leu Leu Ile Ser Met Gly Leu Ser
        35                  40                  45              
Leu Leu Leu Leu Val Val Val Cys Val Ile Gly Ser Gln Asn Ser Lys
    50                  55                  60                  
Leu Gln Glu Glu Leu Gln Ala Leu Arg Glu Thr Phe Ser Asn Leu Thr
65                  70                  75                  80  
Ala Ser Thr Asp Ala Lys Val Lys Thr Leu Ser Met Gln Gly Gly Asn
                85                  90                  95      
Val Gly Arg Lys Met Lys Ser Leu Glu Ser Gln Leu Glu Lys Gln Gln
            100                 105                 110         
Gln Asp Leu Ser Glu Asp His Ser Ser Leu Leu Leu His Val Lys Gln
        115                 120                 125             
Phe Val Ser Asp Leu Arg Ser Leu Ser Cys Gln Met Ala Val Leu Gln
    130                 135                 140                 
Gly Asn Gly Ser Glu Arg Thr Cys Cys Pro Val Asn Trp Val Gly Tyr
145                 150                 155                 160 
Glu Gly Ser Cys Tyr Trp Phe Ser Arg Ser Gly Lys Pro Trp Pro Glu
                165                 170                 175     
Ala Glu Lys Tyr Cys Gln Leu Glu Asn Ala His Leu Val Val Val Gly
            180                 185                 190         
Ser Trp Glu Glu Gln Lys Phe Ile Gln His His Val Gly Pro Val Asn
        195                 200                 205             
Ser Trp Ile Gly Leu Thr Asp Gln Ser Gly Pro Trp Lys Trp Val Asp
    210                 215                 220                 
Gly Thr Asp Tyr Glu Ser Gly Phe Lys Asn Trp Arg Pro Glu Gln Pro
225                 230                 235                 240 
Asp Asp Trp Tyr Gly His Gly Leu Gly Gly Gly Glu Asp Cys Ala His
                245                 250                 255     
Phe Thr Glu Asp Gly Gly Trp Asn Asp Asp Ile Cys Gln Arg Pro Tyr
            260                 265                 270         
Arg Trp Val Cys Glu Thr Gln Arg Asp Arg Asp Ser Gly Ser
        275                 280                 285     


<210> 11
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> siRNA sense sequence

<400> 11
cagcaugcag ggaggaaauu u                                           21

<210> 12
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> siRNA ASGR1 antisense sequence

<400> 12
auuuccuccc ugcaugcugu u                                           21

<210> 13
<211> 33
<212> PRT
<213> Homo sapiens

<400> 13
Arg Pro Glu Gln Pro Asp Asp Trp Tyr Gly His Gly Leu Gly Gly Gly
 1               5                  10                  15      
Glu Asp Cys Ala His Phe Thr Asp Asp Gly Arg Trp Asn Asp Asp Val
            20                  25                  30          
Cys
    

