                         SEQUENCE LISTING

<110>  Dana-Farber Cancer Institute, Inc.
 
<120>  CRLF2 IN PRECURSOR B-CELL ACUTE LYMPHOBLASTIC LEUKEMIA

<130>  D0504.70013WO00

<140>  Not yet assigned
<141>  Concurrently Herewith

<150>  US 61/264,315
<151>  2009-11-25

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  371
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Gly Arg Leu Val Leu Leu Trp Gly Ala Ala Val Phe Leu Leu Gly 
1               5                   10                  15      


Gly Trp Met Ala Leu Gly Gln Gly Gly Ala Ala Glu Gly Val Gln Ile 
            20                  25                  30          


Gln Ile Ile Tyr Phe Asn Leu Glu Thr Val Gln Val Thr Trp Asn Ala 
        35                  40                  45              


Ser Lys Tyr Ser Arg Thr Asn Leu Thr Phe His Tyr Arg Phe Asn Gly 
    50                  55                  60                  


Asp Glu Ala Tyr Asp Gln Cys Thr Asn Tyr Leu Leu Gln Glu Gly His 
65                  70                  75                  80  


Thr Ser Gly Cys Leu Leu Asp Ala Glu Gln Arg Asp Asp Ile Leu Tyr 
                85                  90                  95      


Phe Ser Ile Arg Asn Gly Thr His Pro Val Phe Thr Ala Ser Arg Trp 
            100                 105                 110         


Met Val Tyr Tyr Leu Lys Pro Ser Ser Pro Lys His Val Arg Phe Ser 
        115                 120                 125             


Trp His Gln Asp Ala Val Thr Val Thr Cys Ser Asp Leu Ser Tyr Gly 
    130                 135                 140                 


Asp Leu Leu Tyr Glu Val Gln Tyr Arg Ser Pro Phe Asp Thr Glu Trp 
145                 150                 155                 160 


Gln Ser Lys Gln Glu Asn Thr Cys Asn Val Thr Ile Glu Gly Leu Asp 
                165                 170                 175     


Ala Glu Lys Cys Tyr Ser Phe Trp Val Arg Val Lys Ala Met Glu Asp 
            180                 185                 190         


Val Tyr Gly Pro Asp Thr Tyr Pro Ser Asp Trp Ser Glu Val Thr Cys 
        195                 200                 205             


Trp Gln Arg Gly Glu Ile Arg Asp Ala Cys Ala Glu Thr Pro Thr Pro 
    210                 215                 220                 


Pro Lys Pro Lys Leu Ser Lys Phe Ile Leu Ile Ser Ser Leu Ala Ile 
225                 230                 235                 240 


Leu Leu Met Val Ser Leu Leu Leu Leu Ser Leu Trp Lys Leu Trp Arg 
                245                 250                 255     


Val Lys Lys Phe Leu Ile Pro Ser Val Pro Asp Pro Lys Ser Ile Phe 
            260                 265                 270         


Pro Gly Leu Phe Glu Ile His Gln Gly Asn Phe Gln Glu Trp Ile Thr 
        275                 280                 285             


Asp Thr Gln Asn Val Ala His Leu His Lys Met Ala Gly Ala Glu Gln 
    290                 295                 300                 


Glu Ser Gly Pro Glu Glu Pro Leu Val Val Gln Leu Ala Lys Thr Glu 
305                 310                 315                 320 


Ala Glu Ser Pro Arg Met Leu Asp Pro Gln Thr Glu Glu Lys Glu Ala 
                325                 330                 335     


Ser Gly Gly Ser Leu Gln Leu Pro His Gln Pro Leu Gln Gly Gly Asp 
            340                 345                 350         


Val Val Thr Ile Gly Gly Phe Thr Phe Val Met Asn Asp Arg Ser Tyr 
        355                 360                 365             


Val Ala Leu 
    370     


<210>  2
<211>  1579
<212>  DNA
<213>  Homo sapiens

<400>  2
aattcggcac gagggcatgg ggcggctggt tctgctgtgg ggagctgccg tctttctgct       60

gggaggctgg atggctttgg ggcaaggagg agcagcagaa ggagtacaga ttcagatcat      120

ctacttcaat ttagaaaccg tgcaggtgac atggaatgcc agcaaatact ccaggaccaa      180

cctgactttc cactacagat tcaacggtga tgaggcctat gaccagtgca ccaactacct      240

tctccaggaa ggtcacactt cggggtgcct cctagacgca gagcagcgag acgacattct      300

ctatttctcc atcaggaatg ggacgcaccc cgttttcacc gcaagtcgct ggatggttta      360

ttacctgaaa cccagttccc cgaagcacgt gagattttcg tggcatcagg atgcagtgac      420

ggtgacgtgt tctgacctgt cctacgggga tctcctctat gaggttcagt accggagccc      480

cttcgacacc gagtggcagt ccaaacagga aaatacctgc aacgtcacca tagaaggctt      540

ggatgccgag aagtgttact ctttctgggt cagggtgaag gctatggagg atgtatatgg      600

gccagacaca tacccaagcg actggtcaga ggtgacatgc tggcagagag gcgagattcg      660

ggatgcctgt gcagagacac caacgcctcc caaaccaaag ctgtccaaat ttattttaat      720

ttccagcctg gccatccttc tgatggtgtc tctcctcctt ctgtctttat ggaaattatg      780

gagagtgaag aagtttctca ttcccagcgt gccagacccg aaatccatct tccccgggct      840

ctttgagata caccaaggga acttccagga gtggatcaca gacacccaga acgtggccca      900

cctccacaag atggcaggtg cagagcaaga aagtggcccc gaggagcccc tggtagtcca      960

gttggccaag actgaagccg agtctcccag gatgctggac ccacagaccg aggagaaaga     1020

ggcctctggg ggatccctcc agcttcccca ccagcccctc caaggcggtg atgtggtcac     1080

aatcgggggc ttcacctttg tgatgaatga ccgctcctac gtggcgttgt gatggacaca     1140

ccactgtcaa agtcaacgtc aggatccacg ttgacattta aagacagagg ggactgtccc     1200

ggggactcca caccaccatg gatgggaagt ctccacgcca atgatggtag gactaggaga     1260

ctctgaagac ccagcctcac cgcctaatgc ggccactgcc ctgctaactt tcccccacat     1320

gagtctctgt gttcaaaggc ttgatggcag atgggagcca attgctccag gagatttact     1380

cccagttcct tttcgtgcct gaacgttgtc acataaaccc caaggcagca cgtccaaaat     1440

gctgtaaaac catcttccca ctctgtgagt ccccagttcc gtccatgtac ctgttccata     1500

gcattggatt ctcggaggat tttttgtctg ttttgagact ccaaaccacc tctaccccta     1560

caaaaaaaaa aaaaaaaaa                                                  1579


<210>  3
<211>  371
<212>  PRT
<213>  Artificial sequence

<220>
<223>  F232C mutant of human CRLF2 polypeptide

<400>  3

Met Gly Arg Leu Val Leu Leu Trp Gly Ala Ala Val Phe Leu Leu Gly 
1               5                   10                  15      


Gly Trp Met Ala Leu Gly Gln Gly Gly Ala Ala Glu Gly Val Gln Ile 
            20                  25                  30          


Gln Ile Ile Tyr Phe Asn Leu Glu Thr Val Gln Val Thr Trp Asn Ala 
        35                  40                  45              


Ser Lys Tyr Ser Arg Thr Asn Leu Thr Phe His Tyr Arg Phe Asn Gly 
    50                  55                  60                  


Asp Glu Ala Tyr Asp Gln Cys Thr Asn Tyr Leu Leu Gln Glu Gly His 
65                  70                  75                  80  


Thr Ser Gly Cys Leu Leu Asp Ala Glu Gln Arg Asp Asp Ile Leu Tyr 
                85                  90                  95      


Phe Ser Ile Arg Asn Gly Thr His Pro Val Phe Thr Ala Ser Arg Trp 
            100                 105                 110         


Met Val Tyr Tyr Leu Lys Pro Ser Ser Pro Lys His Val Arg Phe Ser 
        115                 120                 125             


Trp His Gln Asp Ala Val Thr Val Thr Cys Ser Asp Leu Ser Tyr Gly 
    130                 135                 140                 


Asp Leu Leu Tyr Glu Val Gln Tyr Arg Ser Pro Phe Asp Thr Glu Trp 
145                 150                 155                 160 


Gln Ser Lys Gln Glu Asn Thr Cys Asn Val Thr Ile Glu Gly Leu Asp 
                165                 170                 175     


Ala Glu Lys Cys Tyr Ser Phe Trp Val Arg Val Lys Ala Met Glu Asp 
            180                 185                 190         


Val Tyr Gly Pro Asp Thr Tyr Pro Ser Asp Trp Ser Glu Val Thr Cys 
        195                 200                 205             


Trp Gln Arg Gly Glu Ile Arg Asp Ala Cys Ala Glu Thr Pro Thr Pro 
    210                 215                 220                 


Pro Lys Pro Lys Leu Ser Lys Cys Ile Leu Ile Ser Ser Leu Ala Ile 
225                 230                 235                 240 


Leu Leu Met Val Ser Leu Leu Leu Leu Ser Leu Trp Lys Leu Trp Arg 
                245                 250                 255     


Val Lys Lys Phe Leu Ile Pro Ser Val Pro Asp Pro Lys Ser Ile Phe 
            260                 265                 270         


Pro Gly Leu Phe Glu Ile His Gln Gly Asn Phe Gln Glu Trp Ile Thr 
        275                 280                 285             


Asp Thr Gln Asn Val Ala His Leu His Lys Met Ala Gly Ala Glu Gln 
    290                 295                 300                 


Glu Ser Gly Pro Glu Glu Pro Leu Val Val Gln Leu Ala Lys Thr Glu 
305                 310                 315                 320 


Ala Glu Ser Pro Arg Met Leu Asp Pro Gln Thr Glu Glu Lys Glu Ala 
                325                 330                 335     


Ser Gly Gly Ser Leu Gln Leu Pro His Gln Pro Leu Gln Gly Gly Asp 
            340                 345                 350         


Val Val Thr Ile Gly Gly Phe Thr Phe Val Met Asn Asp Arg Ser Tyr 
        355                 360                 365             


Val Ala Leu 
    370     


<210>  4
<211>  1116
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleic acid encoding mutant human CRLF2 polypeptide

<400>  4
atggggcggc tggttctgct gtggggagct gccgtctttc tgctgggagg ctggatggct       60

ttggggcaag gaggagcagc agaaggagta cagattcaga tcatctactt caatttagaa      120

accgtgcagg tgacatggaa tgccagcaaa tactccagga ccaacctgac tttccactac      180

agattcaacg gtgatgaggc ctatgaccag tgcaccaact accttctcca ggaaggtcac      240

acttcggggt gcctcctaga cgcagagcag cgagacgaca ttctctattt ctccatcagg      300

aatgggacgc accccgtttt caccgcaagt cgctggatgg tttattacct gaaacccagt      360

tccccgaagc acgtgagatt ttcgtggcat caggatgcag tgacggtgac gtgttctgac      420

ctgtcctacg gggatctcct ctatgaggtt cagtaccgga gccccttcga caccgagtgg      480

cagtccaaac aggaaaatac ctgcaacgtc accatagaag gcttggatgc cgagaagtgt      540

tactctttct gggtcagggt gaaggctatg gaggatgtat atgggccaga cacataccca      600

agcgactggt cagaggtgac atgctggcag agaggcgaga ttcgggatgc ctgtgcagag      660

acaccaacgc ctcccaaacc aaagctgtcc aaatgtattt taatttccag cctggccatc      720

cttctgatgg tgtctctcct ccttctgtct ttatggaaat tatggagagt gaagaagttt      780

ctcattccca gcgtgccaga cccgaaatcc atcttccccg ggctctttga gatacaccaa      840

gggaacttcc aggagtggat cacagacacc cagaacgtgg cccacctcca caagatggca      900

ggtgcagagc aagaaagtgg ccccgaggag cccctggtag tccagttggc caagactgaa      960

gccgagtctc ccaggatgct ggacccacag accgaggaga aagaggcctc tgggggatcc     1020

ctccagcttc cccaccagcc cctccaaggc ggtgatgtgg tcacaatcgg gggcttcacc     1080

tttgtgatga atgaccgctc ctacgtggcg ttgtga                               1116


<210>  5
<211>  1116
<212>  DNA
<213>  Homo sapiens

<400>  5
atggggcggc tggttctgct gtggggagct gccgtctttc tgctgggagg ctggatggct       60

ttggggcaag gaggagcagc agaaggagta cagattcaga tcatctactt caatttagaa      120

accgtgcagg tgacatggaa tgccagcaaa tactccagga ccaacctgac tttccactac      180

agattcaacg gtgatgaggc ctatgaccag tgcaccaact accttctcca ggaaggtcac      240

acttcggggt gcctcctaga cgcagagcag cgagacgaca ttctctattt ctccatcagg      300

aatgggacgc accccgtttt caccgcaagt cgctggatgg tttattacct gaaacccagt      360

tccccgaagc acgtgagatt ttcgtggcat caggatgcag tgacggtgac gtgttctgac      420

ctgtcctacg gggatctcct ctatgaggtt cagtaccgga gccccttcga caccgagtgg      480

cagtccaaac aggaaaatac ctgcaacgtc accatagaag gcttggatgc cgagaagtgt      540

tactctttct gggtcagggt gaaggctatg gaggatgtat atgggccaga cacataccca      600

agcgactggt cagaggtgac atgctggcag agaggcgaga ttcgggatgc ctgtgcagag      660

acaccaacgc ctcccaaacc aaagctgtcc aaatttattt taatttccag cctggccatc      720

cttctgatgg tgtctctcct ccttctgtct ttatggaaat tatggagagt gaagaagttt      780

ctcattccca gcgtgccaga cccgaaatcc atcttccccg ggctctttga gatacaccaa      840

gggaacttcc aggagtggat cacagacacc cagaacgtgg cccacctcca caagatggca      900

ggtgcagagc aagaaagtgg ccccgaggag cccctggtag tccagttggc caagactgaa      960

gccgagtctc ccaggatgct ggacccacag accgaggaga aagaggcctc tgggggatcc     1020

ctccagcttc cccaccagcc cctccaaggc ggtgatgtgg tcacaatcgg gggcttcacc     1080

tttgtgatga atgaccgctc ctacgtggcg ttgtga                               1116


