                         SEQUENCE LISTING

<110>  Codexis, Inc.
       Clark, Louis
       Zimmerman, Sabrina
 
<120>  ENGINEERED BETA-CLASS CARBONIC ANHYDRASE POLYPEPTIDES AND USES 
       THEREOF

<130>  CX3-031USP1

<160>  46    

<170>  PatentIn version 3.5

<210>  1
<211>  531
<212>  DNA
<213>  Methanobacterium thermoautotrophicus str. Delta H

<400>  1
atgcgctttg tatctatgat cattaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtgaccttt ctgaccttaa acattcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgatgga gtgatccgct cggcggctgt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagatta a               531


<210>  2
<211>  176
<212>  PRT
<213>  Methanobacterium thermoautotrophicus str. Delta H

<400>  2

Met Arg Phe Val Ser Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Leu Ser Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Asp Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  3
<211>  516
<212>  DNA
<213>  Methanobacterium thermoautotrophicus str. Delta H

<400>  3
atgatcatta aagacattct tcgcgagaac caagacttcc gcttccgtga cctttctgac       60

cttaaacatt caccgaaatt atgtattatt acctgcatgg attcgcgtct catcgatctc      120

ttggagcgtg cattagggat cggacgtggc gatgcgaagg taatcaagaa cgccgggaat      180

attgttgatg atggagtgat ccgctcggcg gctgtagcga tttatgccct gggagttaac      240

gaaattatta ttgtgggcca caccgattgt ggaatggctc gcctcgatga agatctgatt      300

gtatcacgca tgcgcgaact tggcgttgag gaagaagtca ttgagaactt tagcatcgac      360

gtgttaaacc ccgttggcga tgaagaagag aacgtgatcg aaggagtgaa acgccttaag      420

tctagcccgc tgatcccaga aagcattggt gttcatggct taattatcga tatcaatacc      480

ggccgcctga aacctctgta tctggatgaa gattaa                                516


<210>  4
<211>  171
<212>  PRT
<213>  Methanobacterium thermoautotrophicus str. Delta H

<400>  4

Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln Asp Phe Arg Phe Arg 
1               5                   10                  15      


Asp Leu Ser Asp Leu Lys His Ser Pro Lys Leu Cys Ile Ile Thr Cys 
            20                  25                  30          


Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg Ala Leu Gly Ile Gly 
        35                  40                  45              


Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly Asn Ile Val Asp Asp 
    50                  55                  60                  


Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr Ala Leu Gly Val Asn 
65                  70                  75                  80  


Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly Met Ala Arg Leu Asp 
                85                  90                  95      


Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu Gly Val Glu Glu Glu 
            100                 105                 110         


Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn Pro Val Gly Asp Glu 
        115                 120                 125             


Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu Lys Ser Ser Pro Leu 
    130                 135                 140                 


Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile Ile Asp Ile Asn Thr 
145                 150                 155                 160 


Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170     


<210>  5
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  5
atgtgctttg tatctatgat cattaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtgaccttt ctgaccttaa acattcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgatgga gtgatccgct cggcggctgt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  6
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  6

Met Cys Phe Val Ser Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Leu Ser Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Asp Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  7
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  7
atgcgctttg tatctatgat cattaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtgaccttt ctcctcttaa acattcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgatgga gtgatccgct cggcggctgt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  8
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  8

Met Arg Phe Val Ser Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Leu Ser Pro Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Asp Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  9
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  9
atgcgctttg tatctatgat cattaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtgaccttt ctgaccttaa acattcaccg aaattatgta ttattacctg catggatacc      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgatgga gtgatccgct cggcggctgt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  10
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  10

Met Arg Phe Val Ser Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Leu Ser Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Thr Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Asp Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  11
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  11
atgcgctttg tatctatgat cattaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtgaccttt ctgaccttaa acattcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcaagc gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgatgga gtgatccgct cggcggctgt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  12
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  12

Met Arg Phe Val Ser Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Leu Ser Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Lys Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Asp Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  13
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  13
atgcgctttg tatctatgat cattaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtgaccttt ctgaccttaa acattcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgaggga gtgatccgct cggcggctgt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  14
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  14

Met Arg Phe Val Ser Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Leu Ser Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Glu Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  15
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  15
atgcgctttg tatctatgat cattaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtgaccttt ctgaccttaa acattcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgatgga gtgatccgct cggcggctgt agcgatttat      240

gccctggggg ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctca tccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  16
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  16

Met Arg Phe Val Ser Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Leu Ser Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Asp Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser His Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  17
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  17
atgcgctttg tatctatgat cattaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtgaccttt ctgaccttaa acattcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgatgga gtgatccgct cggcggctgt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttcctgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  18
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  18

Met Arg Phe Val Ser Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Leu Ser Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Asp Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Pro Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  19
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  19
atgcgctttg tatctatgat cattaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtgaccttt ctgaccttaa acattcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgatgga gtgatccgct cggcggctgt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttggtgttca tggctcaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  20
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  20

Met Arg Phe Val Ser Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Leu Ser Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Asp Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Ser Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  21
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  21
atgcgctttg tatctatgat cttgaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtgaccttt ctgaccttaa acattcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgatgga gtgatccgct cggcggctgt agcgatttat      240

gccctgggag ttaaggaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  22
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  22

Met Arg Phe Val Ser Met Ile Leu Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Leu Ser Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Asp Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Lys Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  23
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  23
atgcgctttg tatctatgat cattaaaggc attcttcgcg agaaccaaga cttccgcttc       60

cgtgaccttt ctgaccttaa acattcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgatgga gtgatccgct cgctggctgt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  24
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  24

Met Arg Phe Val Ser Met Ile Ile Lys Gly Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Leu Ser Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Asp Gly Val Ile Arg Ser Leu Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  25
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  25
atgcgctttg tatctatgat cattaaagac attcttcgcg agaaccaagt cttccgcttc       60

cgtgaccttt ctgaccttaa acattcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgaggga gtgatccgct cggcggctgt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  26
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  26

Met Arg Phe Val Ser Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Val Phe Arg Phe Arg Asp Leu Ser Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Glu Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  27
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  27
atgcgctttg tatctatgat cattaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtgtccttt ctgaccttaa acattcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgaggga gtgatccgct cggcggctgt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  28
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  28

Met Arg Phe Val Ser Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Val Leu Ser Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Glu Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  29
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  29
atgcgctttg tatctatgat cattaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtgactact ctgaccttaa acattcaccg aaattatgta ttattacctg catggatacc      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgatgga gtgatccgct cggcggctgt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  30
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  30

Met Arg Phe Val Ser Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Tyr Ser Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Thr Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Asp Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  31
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  31
atgcgctttg tatctatgat cattaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtgactact ctgaccttaa acattcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgatgga gtgatccgct cggcggctgt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttaaga tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  32
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  32

Met Arg Phe Val Ser Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Tyr Ser Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Asp Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Lys Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  33
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  33
atgcgctttg tatctatgat cattaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtgaccttg aagaccttaa acattcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgatgga gtgatccgct cggcggctgt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgcatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  34
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  34

Met Arg Phe Val Ser Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Leu Glu Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Asp Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Ala Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  35
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  35
atgcgctttg tatctatgat cattaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtgaccttt ctgaccttaa acattcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgaggga gtgatccgct cggcggctgt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctca tccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  36
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  36

Met Arg Phe Val Ser Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Leu Ser Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Glu Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser His Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  37
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  37
atgcgctttg tatctatgat cattaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtgaccttt ctgaccttaa acattcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgaggga gtgatccgct cggcggctgt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaagata ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  38
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  38

Met Arg Phe Val Ser Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Leu Ser Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Glu Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Asp Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  39
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  39
atgcgctttg tatctatgat cttgaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtgaccttt ctgaccttaa acattcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgatgga gtgatccgct cggcggctgt agcgatttat      240

gccctgggag ttaaggaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacttctt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  40
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  40

Met Arg Phe Val Ser Met Ile Leu Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Leu Ser Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Asp Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Lys Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Phe Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  41
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  41
atgcgctttg tatctatgat cattaaagac attctttgcg agaaccaaga cttccgcttc       60

cgtgaccttt ctgaccttaa acattcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcacta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgatgga gtgatccgct cggcggtggt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacacgg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

gactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  42
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  42

Met Arg Phe Val Ser Met Ile Ile Lys Asp Ile Leu Cys Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Leu Ser Asp Leu Lys His Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Asp Gly Val Ile Arg Ser Ala Val Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asp Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  43
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  43
atgcgctttg tatctatgat cattaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtggccttt ctgaccttaa acattcaccg aaatcatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgatgga gtgatccgct cgctggctgt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  44
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  44

Met Arg Phe Val Ser Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Gly Leu Ser Asp Leu Lys His Ser Pro Lys Ser 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Asp Gly Val Ile Arg Ser Leu Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


<210>  45
<211>  528
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  45
atgcgctttg tatctatgat cattaaagac attcttcgcg agaaccaaga cttccgcttc       60

cgtgaccttt ctgaccttaa accgtcaccg aaattatgta ttattacctg catggattcg      120

cgtctcatcg atctcttgga gcgtgcatta gggatcggac gtggcgatgc gaaggtaatc      180

aagaacgccg ggaatattgt tgatgatgga gtgatccgct cggcggctgt agcgatttat      240

gccctgggag ttaacgaaat tattattgtg ggccacaccg attgtggaat ggctcgcctc      300

gatgaagatc tgattgtatc acgcatgcgc gaacttggcg ttgaggaaga agtcattgag      360

aactttagca tcgacgtgtt aaaccccgtt ggcgatgaag aagagaacgt gatcgaagga      420

gtgaaacgcc ttaagtctag cccgctgatc ccagaaagca ttggtgttca tggcttaatt      480

atcgatatca ataccggccg cctgaaacct ctgtatctgg atgaagat                   528


<210>  46
<211>  176
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Variant of M. thermautotrophicus str. Delta H carbonic anhydrase

<400>  46

Met Arg Phe Val Ser Met Ile Ile Lys Asp Ile Leu Arg Glu Asn Gln 
1               5                   10                  15      


Asp Phe Arg Phe Arg Asp Leu Ser Asp Leu Lys Pro Ser Pro Lys Leu 
            20                  25                  30          


Cys Ile Ile Thr Cys Met Asp Ser Arg Leu Ile Asp Leu Leu Glu Arg 
        35                  40                  45              


Ala Leu Gly Ile Gly Arg Gly Asp Ala Lys Val Ile Lys Asn Ala Gly 
    50                  55                  60                  


Asn Ile Val Asp Asp Gly Val Ile Arg Ser Ala Ala Val Ala Ile Tyr 
65                  70                  75                  80  


Ala Leu Gly Val Asn Glu Ile Ile Ile Val Gly His Thr Asp Cys Gly 
                85                  90                  95      


Met Ala Arg Leu Asp Glu Asp Leu Ile Val Ser Arg Met Arg Glu Leu 
            100                 105                 110         


Gly Val Glu Glu Glu Val Ile Glu Asn Phe Ser Ile Asp Val Leu Asn 
        115                 120                 125             


Pro Val Gly Asp Glu Glu Glu Asn Val Ile Glu Gly Val Lys Arg Leu 
    130                 135                 140                 


Lys Ser Ser Pro Leu Ile Pro Glu Ser Ile Gly Val His Gly Leu Ile 
145                 150                 155                 160 


Ile Asp Ile Asn Thr Gly Arg Leu Lys Pro Leu Tyr Leu Asp Glu Asp 
                165                 170                 175     


