                         SEQUENCE LISTING

<110>  National Jewish Health
       LENZ, Laurel L.
       SCHMIDT, Rebecca
 
<120>  COMPOSITIONS, METHODS AND USES FOR STIMULATING IMMUNE RESPONSES

<130>  66888-394491

<150>  61/259890
<151>  2009-11-10

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  450
<212>  PRT
<213>  Listeria monocytogenes p60 mutant

<400>  1

Thr Val Val Val Glu Ala Gly Asp Thr Leu Trp Gly Ile Ala Gln Ser 
1               5                   10                  15      


Lys Gly Thr Thr Val Asp Ala Ile Lys Lys Ala Asn Asn Leu Thr Thr 
            20                  25                  30          


Asp Lys Ile Val Pro Gly Gln Lys Leu Gln Val Asn Asn Glu Val Ala 
        35                  40                  45              


Ala Ala Glu Lys Thr Glu Lys Ser Val Ser Ala Thr Trp Leu Asn Val 
    50                  55                  60                  


Arg Thr Gly Ala Gly Val Asp Asn Ser Ile Ile Thr Ser Ile Lys Gly 
65                  70                  75                  80  


Gly Thr Lys Val Thr Val Glu Thr Thr Glu Ser Asn Gly Trp His Lys 
                85                  90                  95      


Ile Thr Tyr Asn Asp Gly Lys Thr Gly Phe Val Asn Gly Lys Tyr Leu 
            100                 105                 110         


Thr Asp Lys Ala Val Ser Thr Pro Val Ala Pro Thr Gln Glu Val Lys 
        115                 120                 125             


Lys Glu Thr Thr Thr Gln Gln Ala Ala Pro Val Ala Glu Thr Lys Thr 
    130                 135                 140                 


Glu Val Lys Gln Thr Thr Gln Ala Thr Thr Pro Ala Pro Lys Val Ala 
145                 150                 155                 160 


Glu Thr Lys Glu Thr Pro Val Ile Asp Gln Asn Ala Thr Thr His Ala 
                165                 170                 175     


Val Lys Ser Gly Asp Thr Ile Trp Ala Leu Ser Val Lys Tyr Gly Val 
            180                 185                 190         


Ser Val Gln Asp Ile Met Ser Trp Asn Asn Leu Ser Ser Ser Ser Ile 
        195                 200                 205             


Tyr Val Gly Gln Lys Leu Ala Ile Lys Gln Thr Ala Asn Thr Ala Thr 
    210                 215                 220                 


Pro Lys Ala Glu Val Lys Thr Glu Ala Pro Ala Ala Glu Lys Gln Ala 
225                 230                 235                 240 


Ala Pro Val Val Lys Glu Asn Thr Asn Thr Asn Thr Ala Thr Thr Glu 
                245                 250                 255     


Lys Lys Glu Thr Ala Thr Gln Gln Gln Thr Ala Pro Lys Ala Pro Thr 
            260                 265                 270         


Glu Ala Ala Lys Pro Ala Pro Ala Pro Ser Thr Asn Thr Asn Ala Asn 
        275                 280                 285             


Lys Thr Asn Thr Asn Thr Asn Thr Asn Asn Thr Asn Thr Pro Ser Lys 
    290                 295                 300                 


Asn Thr Asn Thr Asn Ser Asn Thr Asn Thr Asn Thr Asn Ser Asn Thr 
305                 310                 315                 320 


Asn Ala Asn Gln Gly Ser Ser Asn Asn Asn Ser Asn Ser Ser Ala Ser 
                325                 330                 335     


Ala Ile Ile Ala Glu Ala Gln Lys His Leu Gly Lys Ala Tyr Ser Trp 
            340                 345                 350         


Gly Gly Asn Gly Pro Thr Thr Phe Asp Ala Ser Gly Tyr Thr Lys Tyr 
        355                 360                 365             


Val Phe Ala Lys Ala Gly Ile Ser Leu Pro Arg Thr Ser Gly Ala Gln 
    370                 375                 380                 


Tyr Ala Ser Thr Thr Arg Ile Ser Glu Ser Gln Ala Lys Pro Gly Asp 
385                 390                 395                 400 


Leu Val Phe Phe Asp Tyr Gly Ser Gly Ile Ser His Ile Gly Ile Tyr 
                405                 410                 415     


Val Gly Asn Gly Gln Met Ile Asn Ala Gln Asp Asn Gly Val Lys Tyr 
            420                 425                 430         


Asp Asn Ile His Gly Ser Gly Trp Gly Lys Tyr Leu Val Gly Phe Gly 
        435                 440                 445             


Arg Val 
    450 


<210>  2
<211>  450
<212>  PRT
<213>  Listeria monocytogenes p60 native

<400>  2

Thr Val Val Val Glu Ala Gly Asp Thr Leu Trp Gly Ile Ala Gln Ser 
1               5                   10                  15      


Lys Gly Thr Thr Val Asp Ala Ile Lys Lys Ala Asn Asn Leu Thr Thr 
            20                  25                  30          


Asp Lys Ile Val Pro Gly Gln Lys Leu Gln Val Asn Asn Glu Val Ala 
        35                  40                  45              


Ala Ala Glu Lys Thr Glu Lys Ser Val Ser Ala Thr Trp Leu Asn Val 
    50                  55                  60                  


Arg Thr Gly Ala Gly Val Asp Asn Ser Ile Ile Thr Ser Ile Lys Gly 
65                  70                  75                  80  


Gly Thr Lys Val Thr Val Glu Thr Thr Glu Ser Asn Gly Trp His Lys 
                85                  90                  95      


Ile Thr Tyr Asn Asp Gly Lys Thr Gly Phe Val Asn Gly Lys Tyr Leu 
            100                 105                 110         


Thr Asp Lys Ala Val Ser Thr Pro Val Ala Pro Thr Gln Glu Val Lys 
        115                 120                 125             


Lys Glu Thr Thr Thr Gln Gln Ala Ala Pro Val Ala Glu Thr Lys Thr 
    130                 135                 140                 


Glu Val Lys Gln Thr Thr Gln Ala Thr Thr Pro Ala Pro Lys Val Ala 
145                 150                 155                 160 


Glu Thr Lys Glu Thr Pro Val Ile Asp Gln Asn Ala Thr Thr His Ala 
                165                 170                 175     


Val Lys Ser Gly Asp Thr Ile Trp Ala Leu Ser Val Lys Tyr Gly Val 
            180                 185                 190         


Ser Val Gln Asp Ile Met Ser Trp Asn Asn Leu Ser Ser Ser Ser Ile 
        195                 200                 205             


Tyr Val Gly Gln Lys Leu Ala Ile Lys Gln Thr Ala Asn Thr Ala Thr 
    210                 215                 220                 


Pro Lys Ala Glu Val Lys Thr Glu Ala Pro Ala Ala Glu Lys Gln Ala 
225                 230                 235                 240 


Ala Pro Val Val Lys Glu Asn Thr Asn Thr Asn Thr Ala Thr Thr Glu 
                245                 250                 255     


Lys Lys Glu Thr Ala Thr Gln Gln Gln Thr Ala Pro Lys Ala Pro Thr 
            260                 265                 270         


Glu Ala Ala Lys Pro Ala Pro Ala Pro Ser Thr Asn Thr Asn Ala Asn 
        275                 280                 285             


Lys Thr Asn Thr Asn Thr Asn Thr Asn Asn Thr Asn Thr Pro Ser Lys 
    290                 295                 300                 


Asn Thr Asn Thr Asn Ser Asn Thr Asn Thr Asn Thr Asn Ser Asn Thr 
305                 310                 315                 320 


Asn Ala Asn Gln Gly Ser Ser Asn Asn Asn Ser Asn Ser Ser Ala Ser 
                325                 330                 335     


Ala Ile Ile Ala Glu Ala Gln Lys His Leu Gly Lys Ala Tyr Ser Trp 
            340                 345                 350         


Gly Gly Asn Gly Pro Thr Thr Phe Asp Cys Ser Gly Tyr Thr Lys Tyr 
        355                 360                 365             


Val Phe Ala Lys Ala Gly Ile Ser Leu Pro Arg Thr Ser Gly Ala Gln 
    370                 375                 380                 


Tyr Ala Ser Thr Thr Arg Ile Ser Glu Ser Gln Ala Lys Pro Gly Asp 
385                 390                 395                 400 


Leu Val Phe Phe Asp Tyr Gly Ser Gly Ile Ser His Ile Gly Ile Tyr 
                405                 410                 415     


Val Gly Asn Gly Gln Met Ile Asn Ala Gln Asp Asn Gly Val Lys Tyr 
            420                 425                 430         


Asp Asn Ile His Gly Ser Gly Trp Gly Lys Tyr Leu Val Gly Phe Gly 
        435                 440                 445             


Arg Val 
    450 


<210>  3
<211>  1353
<212>  DNA
<213>  Listeria monocytogenes p60 mutant

<400>  3
actgtagtag tcgaagctgg tgatactctt tggggtatcg cacaaagtaa agggactact       60

gttgacgcaa ttaaaaaagc aaacaattta acaacagata aaatcgtacc aggtcaaaaa      120

ttacaagtaa ataatgaggt tgctgctgct gaaaaaacag agaaatctgt tagcgcaact      180

tggttaaacg tccgtactgg cgctggtgtt gataacagta ttattacgtc catcaaaggt      240

ggaacaaaag taactgttga aacaaccgaa tctaacggct ggcacaaaat tacttacaac      300

gatggaaaaa ctggtttcgt taacggtaaa tacttaactg acaaagcagt aagcactcca      360

gttgcaccaa cacaagaagt gaaaaaagaa actactactc aacaagctgc acctgttgca      420

gaaacaaaaa ctgaagtaaa acaaactaca caagcaacta cacctgcgcc taaagtagca      480

gaaacgaaag aaactccagt aatagatcaa aatgctacta cacacgctgt caaaagcggt      540

gacactattt gggctttatc cgtaaaatac ggtgtttctg ttcaagacat tatgtcatgg      600

aataatttat cttcttcttc tatttatgta ggtcaaaagc ttgctattaa acaaactgct      660

aacacagcta ctccaaaagc agaagtgaaa acggaagctc cagcagctga aaaacaagca      720

gctccagtag ttaaagaaaa tactaacaca aatactgcta ctacagagaa aaaagaaaca      780

gcaacgcaac aacaaacagc acctaaagca ccaacagaag ctgcaaaacc agctcctgca      840

ccatctacaa acacaaatgc taataaaacg aatacaaata caaatacaaa caatactaat      900

acaccatcta aaaatactaa tacaaactca aatactaata cgaatacaaa ctcaaatacg      960

aatgctaatc aaggttcttc caacaataac agcaattcaa gtgcaagtgc tattattgct     1020

gaagctcaaa aacaccttgg aaaagcttat tcatggggtg gtaacggacc aactacattt     1080

gatgcctctg gttacactaa atatgtattt gctaaagcgg gtatctccct tccacgtaca     1140

tctggcgcac aatatgctag cactacaaga atttctgaat ctcaagcaaa acctggtgat     1200

ttagtattct tcgactatgg tagcggaatt tctcacgttg gtatttatgt tggtaatggt     1260

caaatgatta acgcgcaaga caatggcgtt aaatacgata acatccacgg ctctggctgg     1320

ggtaaatatc tagttggctt cggtcgcgta taa                                  1353


<210>  4
<211>  1353
<212>  DNA
<213>  Listeria monocytogenes p60 native

<400>  4
actgtagtag tcgaagctgg tgatactctt tggggtatcg cacaaagtaa agggactact       60

gttgacgcaa ttaaaaaagc aaacaattta acaacagata aaatcgtacc aggtcaaaaa      120

ttacaagtaa ataatgaggt tgctgctgct gaaaaaacag agaaatctgt tagcgcaact      180

tggttaaacg tccgtactgg cgctggtgtt gataacagta ttattacgtc catcaaaggt      240

ggaacaaaag taactgttga aacaaccgaa tctaacggct ggcacaaaat tacttacaac      300

gatggaaaaa ctggtttcgt taacggtaaa tacttaactg acaaagcagt aagcactcca      360

gttgcaccaa cacaagaagt gaaaaaagaa actactactc aacaagctgc acctgttgca      420

gaaacaaaaa ctgaagtaaa acaaactaca caagcaacta cacctgcgcc taaagtagca      480

gaaacgaaag aaactccagt aatagatcaa aatgctacta cacacgctgt caaaagcggt      540

gacactattt gggctttatc cgtaaaatac ggtgtttctg ttcaagacat tatgtcatgg      600

aataatttat cttcttcttc tatttatgta ggtcaaaagc ttgctattaa acaaactgct      660

aacacagcta ctccaaaagc agaagtgaaa acggaagctc cagcagctga aaaacaagca      720

gctccagtag ttaaagaaaa tactaacaca aatactgcta ctacagagaa aaaagaaaca      780

gcaacgcaac aacaaacagc acctaaagca ccaacagaag ctgcaaaacc agctcctgca      840

ccatctacaa acacaaatgc taataaaacg aatacaaata caaatacaaa caatactaat      900

acaccatcta aaaatactaa tacaaactca aatactaata cgaatacaaa ctcaaatacg      960

aatgctaatc aaggttcttc caacaataac agcaattcaa gtgcaagtgc tattattgct     1020

gaagctcaaa aacaccttgg aaaagcttat tcatggggtg gtaacggacc aactacattt     1080

gattgctctg gttacactaa atatgtattt gctaaagcgg gtatctccct tccacgtaca     1140

tctggcgcac aatatgctag cactacaaga atttctgaat ctcaagcaaa acctggtgat     1200

ttagtattct tcgactatgg tagcggaatt tctcacgttg gtatttatgt tggtaatggt     1260

caaatgatta acgcgcaaga caatggcgtt aaatacgata acatccacgg ctctggctgg     1320

ggtaaatatc tagttggctt cggtcgcgta taa                                  1353


<210>  5
<211>  476
<212>  PRT
<213>  Listeria monocytogenes p60 native with leader

<400>  5

Met Lys Lys Ala Thr Ile Ala Ala Thr Ala Gly Ile Ala Val Thr Ala 
1               5                   10                  15      


Phe Ala Ala Pro Thr Ile Ala Ser Ala Ser Thr Val Val Val Glu Ala 
            20                  25                  30          


Gly Asp Thr Leu Trp Gly Ile Ala Gln Ser Lys Gly Thr Thr Val Asp 
        35                  40                  45              


Ala Ile Lys Lys Ala Asn Asn Leu Thr Thr Asp Lys Ile Val Pro Gly 
    50                  55                  60                  


Gln Lys Leu Gln Val Asn Asn Glu Val Ala Ala Ala Glu Lys Thr Glu 
65                  70                  75                  80  


Lys Ser Val Ser Ala Thr Trp Leu Asn Val Arg Thr Gly Ala Gly Val 
                85                  90                  95      


Asp Asn Ser Ile Ile Thr Ser Ile Lys Gly Gly Thr Lys Val Thr Val 
            100                 105                 110         


Glu Thr Thr Glu Ser Asn Gly Trp His Lys Ile Thr Tyr Asn Asp Gly 
        115                 120                 125             


Lys Thr Gly Phe Val Asn Gly Lys Tyr Leu Thr Asp Lys Ala Val Ser 
    130                 135                 140                 


Thr Pro Val Ala Pro Thr Gln Glu Val Lys Lys Glu Thr Thr Thr Gln 
145                 150                 155                 160 


Gln Ala Ala Pro Val Ala Glu Thr Lys Thr Glu Val Lys Gln Thr Thr 
                165                 170                 175     


Gln Ala Thr Thr Pro Ala Pro Lys Val Ala Glu Thr Lys Glu Thr Pro 
            180                 185                 190         


Val Ile Asp Gln Asn Ala Thr Thr His Ala Val Lys Ser Gly Asp Thr 
        195                 200                 205             


Ile Trp Ala Leu Ser Val Lys Tyr Gly Val Ser Val Gln Asp Ile Met 
    210                 215                 220                 


Ser Trp Asn Asn Leu Ser Ser Ser Ser Ile Tyr Val Gly Gln Lys Leu 
225                 230                 235                 240 


Ala Ile Lys Gln Thr Ala Asn Thr Ala Thr Pro Lys Ala Glu Val Lys 
                245                 250                 255     


Thr Glu Ala Pro Ala Ala Glu Lys Gln Ala Ala Pro Val Val Lys Glu 
            260                 265                 270         


Asn Thr Asn Thr Asn Thr Ala Thr Thr Glu Lys Lys Glu Thr Ala Thr 
        275                 280                 285             


Gln Gln Gln Thr Ala Pro Lys Ala Pro Thr Glu Ala Ala Lys Pro Ala 
    290                 295                 300                 


Pro Ala Pro Ser Thr Asn Thr Asn Ala Asn Lys Thr Asn Thr Asn Thr 
305                 310                 315                 320 


Asn Thr Asn Asn Thr Asn Thr Pro Ser Lys Asn Thr Asn Thr Asn Ser 
                325                 330                 335     


Asn Thr Asn Thr Asn Thr Asn Ser Asn Thr Asn Ala Asn Gln Gly Ser 
            340                 345                 350         


Ser Asn Asn Asn Ser Asn Ser Ser Ala Ser Ala Ile Ile Ala Glu Ala 
        355                 360                 365             


Gln Lys His Leu Gly Lys Ala Tyr Ser Trp Gly Gly Asn Gly Pro Thr 
    370                 375                 380                 


Thr Phe Asp Cys Ser Gly Tyr Thr Lys Tyr Val Phe Ala Lys Ala Gly 
385                 390                 395                 400 


Ile Ser Leu Pro Arg Thr Ser Gly Ala Gln Tyr Ala Ser Thr Thr Arg 
                405                 410                 415     


Ile Ser Glu Ser Gln Ala Lys Pro Gly Asp Leu Val Phe Phe Asp Tyr 
            420                 425                 430         


Gly Ser Gly Ile Ser His Val Gly Ile Tyr Val Gly Asn Gly Gln Met 
        435                 440                 445             


Ile Asn Ala Gln Asp Asn Gly Val Lys Tyr Asp Asn Ile His Gly Ser 
    450                 455                 460                 


Gly Trp Gly Lys Tyr Leu Val Gly Phe Gly Arg Val 
465                 470                 475     


<210>  6
<211>  476
<212>  PRT
<213>  Listeria monocytogenes p60 native with leader

<400>  6

Met Lys Lys Ala Thr Ile Ala Ala Thr Ala Gly Ile Ala Val Thr Ala 
1               5                   10                  15      


Phe Ala Ala Pro Thr Ile Ala Ser Ala Ser Thr Val Val Val Glu Ala 
            20                  25                  30          


Gly Asp Thr Leu Trp Gly Ile Ala Gln Ser Lys Gly Thr Thr Val Asp 
        35                  40                  45              


Ala Ile Lys Lys Ala Asn Asn Leu Thr Thr Asp Lys Ile Val Pro Gly 
    50                  55                  60                  


Gln Lys Leu Gln Val Asn Asn Glu Val Ala Ala Ala Glu Lys Thr Glu 
65                  70                  75                  80  


Lys Ser Val Ser Ala Thr Trp Leu Asn Val Arg Thr Gly Ala Gly Val 
                85                  90                  95      


Asp Asn Ser Ile Ile Thr Ser Ile Lys Gly Gly Thr Lys Val Thr Val 
            100                 105                 110         


Glu Thr Thr Glu Ser Asn Gly Trp His Lys Ile Thr Tyr Asn Asp Gly 
        115                 120                 125             


Lys Thr Gly Phe Val Asn Gly Lys Tyr Leu Thr Asp Lys Ala Val Ser 
    130                 135                 140                 


Thr Pro Val Ala Pro Thr Gln Glu Val Lys Lys Glu Thr Thr Thr Gln 
145                 150                 155                 160 


Gln Ala Ala Pro Val Ala Glu Thr Lys Thr Glu Val Lys Gln Thr Thr 
                165                 170                 175     


Gln Ala Thr Thr Pro Ala Pro Lys Val Ala Glu Thr Lys Glu Thr Pro 
            180                 185                 190         


Val Ile Asp Gln Asn Ala Thr Thr His Ala Val Lys Ser Gly Asp Thr 
        195                 200                 205             


Ile Trp Ala Leu Ser Val Lys Tyr Gly Val Ser Val Gln Asp Ile Met 
    210                 215                 220                 


Ser Trp Asn Asn Leu Ser Ser Ser Ser Ile Tyr Val Gly Gln Lys Leu 
225                 230                 235                 240 


Ala Ile Lys Gln Thr Ala Asn Thr Ala Thr Pro Lys Ala Glu Val Lys 
                245                 250                 255     


Thr Glu Ala Pro Ala Ala Glu Lys Gln Ala Ala Pro Val Val Lys Glu 
            260                 265                 270         


Asn Thr Asn Thr Asn Thr Ala Thr Thr Glu Lys Lys Glu Thr Ala Thr 
        275                 280                 285             


Gln Gln Gln Thr Ala Pro Lys Ala Pro Thr Glu Ala Ala Lys Pro Ala 
    290                 295                 300                 


Pro Ala Pro Ser Thr Asn Thr Asn Ala Asn Lys Thr Asn Thr Asn Thr 
305                 310                 315                 320 


Asn Thr Asn Asn Thr Asn Thr Pro Ser Lys Asn Thr Asn Thr Asn Ser 
                325                 330                 335     


Asn Thr Asn Thr Asn Thr Asn Ser Asn Thr Asn Ala Asn Gln Gly Ser 
            340                 345                 350         


Ser Asn Asn Asn Ser Asn Ser Ser Ala Ser Ala Ile Ile Ala Glu Ala 
        355                 360                 365             


Gln Lys His Leu Gly Lys Ala Tyr Ser Trp Gly Gly Asn Gly Pro Thr 
    370                 375                 380                 


Thr Phe Asp Ala Ser Gly Tyr Thr Lys Tyr Val Phe Ala Lys Ala Gly 
385                 390                 395                 400 


Ile Ser Leu Pro Arg Thr Ser Gly Ala Gln Tyr Ala Ser Thr Thr Arg 
                405                 410                 415     


Ile Ser Glu Ser Gln Ala Lys Pro Gly Asp Leu Val Phe Phe Asp Tyr 
            420                 425                 430         


Gly Ser Gly Ile Ser His Val Gly Ile Tyr Val Gly Asn Gly Gln Met 
        435                 440                 445             


Ile Asn Ala Gln Asp Asn Gly Val Lys Tyr Asp Asn Ile His Gly Ser 
    450                 455                 460                 


Gly Trp Gly Lys Tyr Leu Val Gly Phe Gly Arg Val 
465                 470                 475     


<210>  7
<211>  476
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Listeria monocytogenes p60 derivative

<400>  7

Met Lys Lys Ala Thr Ile Ala Ala Thr Ala Gly Ile Ala Val Thr Ala 
1               5                   10                  15      


Phe Ala Ala Pro Thr Ile Ala Ser Ala Ser Thr Val Val Val Glu Ala 
            20                  25                  30          


Gly Asp Thr Leu Trp Gly Ile Ala Gln Ser Lys Gly Thr Thr Val Asp 
        35                  40                  45              


Ala Ile Lys Lys Ala Asn Asn Leu Thr Thr Asp Lys Ile Val Pro Gly 
    50                  55                  60                  


Gln Lys Leu Gln Val Asn Asn Glu Val Ala Ala Ala Glu Lys Thr Glu 
65                  70                  75                  80  


Lys Ser Val Ser Ala Thr Trp Leu Asn Val Arg Thr Gly Ala Gly Val 
                85                  90                  95      


Asp Asn Ser Ile Ile Thr Ser Ile Lys Gly Gly Thr Lys Val Thr Val 
            100                 105                 110         


Glu Thr Thr Glu Ser Asn Gly Trp His Lys Ile Thr Tyr Asn Asp Gly 
        115                 120                 125             


Lys Thr Gly Phe Val Asn Gly Lys Tyr Leu Thr Asp Lys Ala Val Ser 
    130                 135                 140                 


Thr Pro Val Ala Pro Thr Gln Glu Val Lys Lys Glu Thr Thr Thr Gln 
145                 150                 155                 160 


Gln Ala Ala Pro Val Ala Glu Thr Lys Thr Glu Val Lys Gln Thr Thr 
                165                 170                 175     


Gln Ala Thr Thr Pro Ala Pro Lys Val Ala Glu Thr Lys Glu Thr Pro 
            180                 185                 190         


Val Ile Asp Gln Asn Ala Thr Thr His Ala Val Lys Ser Gly Asp Thr 
        195                 200                 205             


Ile Trp Ala Leu Ser Val Lys Tyr Gly Val Ser Val Gln Asp Ile Met 
    210                 215                 220                 


Ser Trp Asn Asn Leu Ser Ser Ser Ser Ile Tyr Val Gly Gln Lys Leu 
225                 230                 235                 240 


Ala Ile Lys Gln Thr Ala Asn Thr Ala Thr Pro Lys Ala Glu Val Lys 
                245                 250                 255     


Thr Glu Ala Pro Ala Ala Glu Lys Gln Ala Ala Pro Val Val Lys Glu 
            260                 265                 270         


Asn Thr Asn Thr Asn Thr Ala Thr Thr Glu Lys Lys Glu Thr Ala Thr 
        275                 280                 285             


Gln Gln Gln Thr Ala Pro Lys Ala Pro Thr Glu Ala Ala Lys Pro Ala 
    290                 295                 300                 


Pro Ala Pro Ser Thr Asn Thr Asn Ala Asn Lys Thr Asn Thr Asn Thr 
305                 310                 315                 320 


Asn Thr Asn Asn Thr Asn Thr Pro Ser Lys Asn Thr Asn Thr Asn Ser 
                325                 330                 335     


Asn Thr Asn Thr Asn Thr Asn Ser Asn Thr Asn Ala Asn Gln Gly Ser 
            340                 345                 350         


Ser Asn Asn Asn Ser Asn Ser Ser Ala Ser Ala Ile Ile Ala Glu Ala 
        355                 360                 365             


Gln Lys His Leu Gly Lys Ala Tyr Ser Trp Gly Gly Asn Gly Pro Thr 
    370                 375                 380                 


Thr Phe Asp Ala Ser Gly Tyr Thr Lys Tyr Val Phe Ala Lys Ala Gly 
385                 390                 395                 400 


Ile Ser Leu Pro Arg Thr Ser Gly Ala Gln Tyr Ala Ser Thr Thr Arg 
                405                 410                 415     


Ile Ser Glu Ser Gln Ala Lys Pro Gly Asp Leu Val Phe Phe Asp Tyr 
            420                 425                 430         


Gly Ser Gly Ile Ser Ala Val Gly Ile Tyr Val Gly Asn Gly Gln Met 
        435                 440                 445             


Ile Asn Ala Gln Asp Asn Gly Val Lys Tyr Asp Asn Ile Ala Gly Ser 
    450                 455                 460                 


Gly Trp Gly Lys Tyr Leu Val Gly Phe Gly Arg Val 
465                 470                 475     


<210>  8
<211>  114
<212>  PRT
<213>  Listeria monocytogenes p60 LysM and SH3 domain

<400>  8

Ser Thr Val Val Val Glu Ala Gly Asp Thr Leu Trp Gly Ile Ala Gln 
1               5                   10                  15      


Ser Lys Gly Thr Thr Val Asp Ala Ile Lys Lys Ala Asn Asn Leu Thr 
            20                  25                  30          


Thr Asp Lys Ile Val Pro Gly Gln Lys Leu Gln Val Asn Asn Glu Val 
        35                  40                  45              


Ala Ala Ala Glu Lys Thr Glu Lys Ser Val Ser Ala Thr Trp Leu Asn 
    50                  55                  60                  


Val Arg Thr Gly Ala Gly Val Asp Asn Ser Ile Ile Thr Ser Ile Lys 
65                  70                  75                  80  


Gly Gly Thr Lys Val Thr Val Glu Thr Thr Glu Ser Asn Gly Trp His 
                85                  90                  95      


Lys Ile Thr Tyr Asn Asp Gly Lys Thr Gly Phe Val Asn Gly Lys Tyr 
            100                 105                 110         


Leu Thr 
        


<210>  9
<211>  159
<212>  PRT
<213>  Listeria monocytogenes p60 SH3 and LysM2 domain

<400>  9

Thr Trp Leu Asn Val Arg Thr Gly Ala Gly Val Asp Asn Ser Ile Ile 
1               5                   10                  15      


Thr Ser Ile Lys Gly Gly Thr Lys Val Thr Val Glu Thr Thr Glu Ser 
            20                  25                  30          


Asn Gly Trp His Lys Ile Thr Tyr Asn Asp Gly Lys Thr Gly Phe Val 
        35                  40                  45              


Asn Gly Lys Tyr Leu Thr Asp Lys Ala Val Ser Thr Pro Val Ala Pro 
    50                  55                  60                  


Thr Gln Glu Val Lys Lys Glu Thr Thr Thr Gln Gln Ala Ala Pro Val 
65                  70                  75                  80  


Ala Glu Thr Lys Thr Glu Val Lys Gln Thr Thr Gln Ala Thr Thr Pro 
                85                  90                  95      


Ala Pro Lys Val Ala Glu Thr Lys Glu Thr Pro Val Ile Asp Gln Asn 
            100                 105                 110         


Ala Thr Thr His Ala Val Lys Ser Gly Asp Thr Ile Trp Ala Leu Ser 
        115                 120                 125             


Val Lys Tyr Gly Val Ser Val Gln Asp Ile Met Ser Trp Asn Asn Leu 
    130                 135                 140                 


Ser Ser Ser Ser Ile Tyr Val Gly Gln Lys Leu Ala Ile Lys Gln 
145                 150                 155                 


<210>  10
<211>  44
<212>  PRT
<213>  Listeria monocytogenes p60 LysM1

<400>  10

Ser Thr Val Val Val Glu Ala Gly Asp Thr Leu Trp Gly Ile Ala Gln 
1               5                   10                  15      


Ser Lys Gly Thr Thr Val Asp Ala Ile Lys Lys Ala Asn Asn Leu Thr 
            20                  25                  30          


Thr Asp Lys Ile Val Pro Gly Gln Lys Leu Gln Val 
        35                  40                  


<210>  11
<211>  44
<212>  PRT
<213>  Listeria monocytogenes p60 LysM2

<400>  11

His Ala Val Lys Ser Gly Asp Thr Ile Trp Ala Leu Ser Val Lys Tyr 
1               5                   10                  15      


Gly Val Ser Val Gln Asp Ile Met Ser Trp Asn Asn Leu Ser Ser Ser 
            20                  25                  30          


Ser Ile Tyr Val Gly Gln Lys Leu Ala Ile Lys Gln 
        35                  40                  


<210>  12
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  listeria mytocytogenes p60 peptide consensus regions


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (12)..(14)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (16)..(25)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (29)..(34)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (36)..(36)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (41)..(42)
<223>  Xaa can be any naturally occurring amino acid

<400>  12

Xaa Xaa Xaa Xaa Xaa Xaa Gly Asp Thr Xaa Trp Xaa Xaa Xaa Lys Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Asn Asn Leu Xaa Xaa Xaa Xaa 
            20                  25                  30          


Xaa Xaa Val Xaa Gly Gln Lys Leu Xaa Xaa 
        35                  40          


<210>  13
<211>  852
<212>  DNA
<213>  Listeria monocytogenes p60 N-terminus

<400>  13
actgtagtag tcgaagctgg tgatactctt tggggtatcg cacaaagtaa agggactact       60

gttgacgcaa ttaaaaaagc aaacaattta acaacagata aaatcgtacc aggtcaaaaa      120

ttacaagtaa ataatgaggt tgctgctgct gaaaaaacag agaaatctgt tagcgcaact      180

tggttaaacg tccgtactgg cgctggtgtt gataacagta ttattacgtc catcaaaggt      240

ggaacaaaag taactgttga aacaaccgaa tctaacggct ggcacaaaat tacttacaac      300

gatggaaaaa ctggtttcgt taacggtaaa tacttaactg acaaagcagt aagcactcca      360

gttgcaccaa cacaagaagt gaaaaaagaa actactactc aacaagctgc acctgttgca      420

gaaacaaaaa ctgaagtaaa acaaactaca caagcaacta cacctgcgcc taaagtagca      480

gaaacgaaag aaactccagt aatagatcaa aatgctacta cacacgctgt caaaagcggt      540

gacactattt gggctttatc cgtaaaatac ggtgtttctg ttcaagacat tatgtcatgg      600

aataatttat cttcttcttc tatttatgta ggtcaaaagc ttgctattaa acaaactgct      660

aacacagcta ctccaaaagc agaagtgaaa acggaagctc cagcagctga aaaacaagca      720

gctccagtag ttaaagaaaa tactaacaca aatactgcta ctacagagaa aaaagaaaca      780

gcaacgcaac aacaaacagc acctaaagca ccaacagaag ctgcaaaacc agctcctgca      840

ccatctacaa ac                                                          852


<210>  14
<211>  285
<212>  PRT
<213>  Listeria monocytogenes p60 N-terminus

<400>  14

Thr Val Val Val Glu Ala Gly Asp Thr Leu Trp Gly Ile Ala Gln Ser 
1               5                   10                  15      


Lys Gly Thr Thr Val Asp Ala Ile Lys Lys Ala Asn Asn Leu Thr Thr 
            20                  25                  30          


Asp Lys Met Ile Val Pro Gly Gln Lys Leu Gln Val Asn Asn Glu Val 
        35                  40                  45              


Ala Ala Ala Glu Lys Thr Glu Lys Ser Val Ser Ala Thr Trp Leu Asn 
    50                  55                  60                  


Val Arg Thr Gly Ala Gly Val Asp Asn Ser Ile Ile Thr Ser Ile Lys 
65                  70                  75                  80  


Gly Gly Thr Lys Val Thr Val Glu Thr Thr Glu Ser Asn Gly Trp His 
                85                  90                  95      


Lys Ile Thr Tyr Asn Asp Gly Lys Thr Gly Phe Val Asn Gly Lys Tyr 
            100                 105                 110         


Leu Thr Asp Lys Ala Val Ser Thr Pro Val Ala Pro Thr Gln Glu Val 
        115                 120                 125             


Lys Lys Glu Thr Thr Thr Gln Gln Ala Ala Pro Val Ala Glu Thr Lys 
    130                 135                 140                 


Thr Glu Val Lys Gln Thr Thr Gln Ala Thr Thr Pro Ala Pro Lys Val 
145                 150                 155                 160 


Ala Glu Thr Lys Glu Thr Pro Val Ile Asp Gln Asn Ala Thr Thr His 
                165                 170                 175     


Ala Val Lys Ser Gly Asp Thr Ile Trp Ala Leu Ser Val Lys Tyr Gly 
            180                 185                 190         


Val Ser Val Gln Asp Ile Met Ser Trp Asn Asn Leu Ser Ser Ser Ser 
        195                 200                 205             


Ile Tyr Val Gly Gln Lys Leu Ala Ile Lys Gln Thr Ala Asn Thr Ala 
    210                 215                 220                 


Thr Pro Lys Ala Glu Val Lys Thr Glu Ala Pro Ala Ala Glu Lys Gln 
225                 230                 235                 240 


Ala Ala Pro Val Val Lys Glu Asn Thr Asn Thr Asn Thr Ala Thr Thr 
                245                 250                 255     


Glu Lys Lys Glu Thr Ala Thr Gln Gln Gln Thr Ala Pro Lys Ala Pro 
            260                 265                 270         


Thr Glu Ala Ala Lys Pro Ala Pro Ala Pro Ser Thr Asn 
        275                 280                 285 


<210>  15
<211>  501
<212>  DNA
<213>  Listeria monocytogenes p60 C-terminus

<400>  15
acaaatgcta ataaaacgaa tacaaataca aatacaaaca atactaatac accatctaaa       60

aatactaata caaactcaaa tactaatacg aatacaaact caaatacgaa tgctaatcaa      120

ggttcttcca acaataacag caattcaagt gcaagtgcta ttattgctga agctcaaaaa      180

caccttggaa aagcttattc atggggtggt aacggaccaa ctacatttga ttgctctggt      240

tacactaaat atgtatttgc taaagcgggt atctcccttc cacgtacatc tggcgcacaa      300

tatgctagca ctacaagaat ttctgaatct caagcaaaac ctggtgattt agtattcttc      360

gactatggta gcggaatttc tcacgttggt atttatgttg gtaatggtca aatgattaac      420

gcgcaagaca atggcgttaa atacgataac atccacggct ctggctgggg taaatatcta      480

gttggcttcg gtcgcgtata a                                                501


<210>  16
<211>  166
<212>  PRT
<213>  Listeria monocytogenes p60 C-terminus

<400>  16

Thr Asn Ala Asn Lys Thr Asn Thr Asn Thr Asn Thr Asn Asn Thr Asn 
1               5                   10                  15      


Thr Pro Ser Lys Asn Thr Asn Thr Asn Ser Asn Thr Asn Thr Asn Thr 
            20                  25                  30          


Asn Ser Asn Thr Asn Ala Asn Gln Gly Ser Ser Asn Asn Asn Ser Asn 
        35                  40                  45              


Ser Ser Ala Ser Ala Ile Ile Ala Glu Ala Gln Lys His Leu Gly Lys 
    50                  55                  60                  


Ala Tyr Ser Trp Gly Gly Asn Gly Pro Thr Thr Phe Asp Cys Ser Gly 
65                  70                  75                  80  


Tyr Thr Lys Tyr Val Phe Ala Lys Ala Gly Ile Ser Leu Pro Arg Thr 
                85                  90                  95      


Ser Gly Ala Gln Tyr Ala Ser Thr Thr Arg Ile Ser Glu Ser Gln Ala 
            100                 105                 110         


Lys Pro Gly Asp Leu Val Phe Phe Asp Tyr Gly Ser Gly Ile Ser His 
        115                 120                 125             


Val Gly Ile Tyr Val Gly Asn Gly Gln Met Ile Asn Ala Gln Asp Asn 
    130                 135                 140                 


Gly Val Lys Tyr Asp Asn Ile His Gly Ser Gly Trp Gly Lys Tyr Leu 
145                 150                 155                 160 


Val Gly Phe Gly Arg Val 
                165     


<210>  17
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p60 primer

<400>  17
agggatccat gaaaaagcaa ctatcgcggc taca                                   34


<210>  18
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer p60

<400>  18
atgatgatgg tgatggtgat ggtgatggct tgcggatgcg attgttgg                    48


<210>  19
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p60 primer

<400>  19
agccatcacc atcaccatca cgtagtcgaa gctggtactc tt                          42


<210>  20
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p60 primer

<400>  20
agctcgagta taccgcaccg aagccaacta gatatttacc                             40


<210>  21
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p60 primer

<400>  21
actgtagtag tcgaagctgg tgatactctt                                        30


<210>  22
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p60 primer

<400>  22
aagagtatca ccagcttcga ctactacagt                                        30


<210>  23
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p60 primer

<400>  23
ccaactccat ttgatgcttc tggttacact aaatatg                                37


<210>  24
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p60 primer

<400>  24
catatttagt gtaaccagaa gcatcaaatg tagttgg                                37


<210>  25
<211>  46
<212>  PRT
<213>  Listeria monocytogenes p60 LysM1 consensus region


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (18)..(19)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (22)..(25)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (31)..(31)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (45)..(45)
<223>  Xaa can be any naturally occurring amino acid

<400>  25

Tyr Xaa Val Xaa Ala Gly Asp Thr Leu Trp Xaa Ile Ala Xaa Xaa Xaa 
1               5                   10                  15      


Gly Xaa Xaa Val Asp Xaa Xaa Xaa Xaa Leu Xaa Lys Xaa Asn Xaa Leu 
            20                  25                  30          


Xaa Thr Xaa Lys Leu Val Xaa Xaa Gly Gln Xaa Leu Xaa Ile 
        35                  40                  45      


<210>  26
<211>  51
<212>  PRT
<213>  Listeria monocytogenes p60 SH3 consensus region


<220>
<221>  misc_feature
<222>  (5)..(12)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (15)..(16)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (18)..(22)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (26)..(29)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (31)..(32)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (37)..(37)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (42)..(43)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (47)..(49)
<223>  Xaa can be any naturally occurring amino acid

<400>  26

Leu Asn Val Arg Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ile Ile Xaa Xaa 
1               5                   10                  15      


Leu Xaa Xaa Xaa Xaa Xaa Val Xaa Val Xaa Xaa Xaa Xaa Gly Xaa Xaa 
            20                  25                  30          


Trp Xaa Lys Ile Xaa Phe Xaa Xaa Gly Xaa Xaa Gly Tyr Val Xaa Xaa 
        35                  40                  45              


Xaa Tyr Val 
    50      


<210>  27
<211>  54
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  listeria mytocytogenes p60 SH3 domain

<400>  27

Thr Trp Leu Asn Val Arg Thr Gly Ala Gly Val Asp Asn Ser Ile Ile 
1               5                   10                  15      


Thr Ser Ile Lys Lys Gly Thr Lys Val Thr Val Glu Thr Thr Glu Ser 
            20                  25                  30          


Asn Gly Trp His Lys Ile Thr Tyr Asn Asp Gly Lys Thr Gly Phe Val 
        35                  40                  45              


Asn Gly Lys Tyr Leu Thr 
    50                  


