
                                SEQUENCE LISTING

<110> University of Kansas
      DESAIRE, Heather
      LAURENCE, Jennifer
      TOUMI, Melinda
      WENKE, Jamie

<120> METHODS OF PRODUCING AND PURIFYING
  PROTEINS

<130> 079463.0112

<150> 61/256,034          
<151> 2010-10-29  

<160> 7

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 197
<212> PRT
<213> Artificial Sequence

<220> 
<223> recombinant human growth hormone including a
      glycosylation site

<400> 1
Met Ala His His His His Phe Pro Thr Ile Pro Leu Ser Arg Leu Phe
 1               5                  10                  15      
Asp Asn Ala Met Leu Arg Ala His Arg Leu His Gln Leu Ala Phe Asp
            20                  25                  30          
Thr Tyr Gln Glu Phe Glu Glu Ala Tyr Ile Pro Lys Glu Gln Lys Tyr
        35                  40                  45              
Ser Phe Leu Gln Asn Pro Gln Thr Ser Leu Cys Phe Ser Glu Ser Ile
    50                  55                  60                  
Pro Thr Pro Ser Asn Arg Glu Glu Thr Gln Gln Lys Ser Asn Leu Glu
65                  70                  75                  80  
Leu Leu Arg Ile Ser Leu Leu Leu Ile Gln Ser Trp Leu Glu Pro Val
                85                  90                  95      
Gln Phe Leu Arg Ser Val Phe Ala Asn Ser Leu Val Tyr Gly Ala Ser
            100                 105                 110         
Asp Ser Asn Val Tyr Asp Leu Leu Lys Asp Leu Glu Glu Gly Ile Gln
        115                 120                 125             
Thr Leu Met Gly Arg Leu Glu Asn Gly Ser Pro Arg Thr Gly Gln Ile
    130                 135                 140                 
Phe Lys Gln Thr Tyr Ser Lys Phe Asp Thr Asn Ser His Asn Asp Asp
145                 150                 155                 160 
Ala Leu Leu Lys Asn Tyr Gly Leu Leu Tyr Cys Phe Arg Lys Asp Met
                165                 170                 175     
Asp Lys Val Glu Thr Phe Leu Arg Ile Val Gln Cys Arg Ser Val Glu
            180                 185                 190         
Gly Ser Cys Gly Phe
        195         


<210> 2
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> DNA primer

<400> 2
atggggaggc tggaaaatgg cagcccccgg actg                             34

<210> 3
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> DNA primer

<400> 3
cagtccgggg gctgccattt tccagcctcc ccat                             34

<210> 4
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> myc-DDK

<400> 4
Asp Tyr Lys Asp Asp Asp Asp Lys
 1               5              


<210> 5
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer for RNASE4

<400> 5
aacattcgta gtatctgcaa caccaccaat atccaatgc                        39

<210> 6
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer for RNASE4

<400> 6
gcattggata ttggtggtgt tgcagatact acgaatgtt                        39

<210> 7
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> start codon and n-linked glycan sequence

<220> 
<221> misc_feature   
<222> 8, 9, 10
<223> n = A,T,C or G

<220> 
<221> misc_feature   
<222> 8, 9, 10
<223> n = A,T,C or G

<400> 7
gaggagannn gccaccatga acaucacgaa caucacgaac aucacgggta cc         52
