                         SEQUENCE LISTING

<110>  FACCHINI, PETER J.
       HAGEL, JILLIAN M.
       MARTIN, VINCENT
       EKINS, ANDREW
       FOSSATI, ELENA
       LAUZON, JEAN-FRANCOIS
       FARROW, SCOTT
 
<120>  THEBAINE 6-O-DEMETHYLASE, PROTOBERBERINE 10 O-DEMETHYLASE AND 
       CODEINE O-DEMETHYLASE FROM PAPAVER SOMNIFERUM

<130>  UNTI.P0096WO

<140>  61/260,659
<141>  2019-11-12

<150>  61/260,647
<151>  2009-11-12

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  364
<212>  PRT
<213>  Papaver somniferum

<400>  1

Met Glu Lys Ala Lys Leu Met Lys Leu Gly Asn Gly Met Glu Ile Pro 
1               5                   10                  15      


Ser Val Gln Glu Leu Ala Lys Leu Thr Leu Ala Glu Ile Pro Ser Arg 
            20                  25                  30          


Tyr Val Cys Ala Asn Glu Asn Leu Leu Leu Pro Met Gly Ala Ser Val 
        35                  40                  45              


Ile Asn Asp His Glu Thr Ile Pro Val Ile Asp Ile Glu Asn Leu Leu 
    50                  55                  60                  


Ser Pro Glu Pro Ile Ile Gly Lys Leu Glu Leu Asp Arg Leu His Phe 
65                  70                  75                  80  


Ala Cys Lys Glu Trp Gly Phe Phe Gln Val Val Asn His Gly Val Asp 
                85                  90                  95      


Ala Ser Leu Val Asp Ser Val Lys Ser Glu Ile Gln Gly Phe Phe Asn 
            100                 105                 110         


Leu Ser Met Asp Glu Lys Thr Lys Tyr Glu Gln Glu Asp Gly Asp Val 
        115                 120                 125             


Glu Gly Phe Gly Gln Gly Phe Ile Glu Ser Glu Asp Gln Thr Leu Asp 
    130                 135                 140                 


Trp Ala Asp Ile Phe Met Met Phe Thr Leu Pro Leu His Leu Arg Lys 
145                 150                 155                 160 


Pro His Leu Phe Ser Lys Leu Pro Val Pro Leu Arg Glu Thr Ile Glu 
                165                 170                 175     


Ser Tyr Ser Ser Glu Met Lys Lys Leu Ser Met Val Leu Phe Asn Lys 
            180                 185                 190         


Met Glu Lys Ala Leu Gln Val Gln Ala Ala Glu Ile Lys Gly Met Ser 
        195                 200                 205             


Glu Val Phe Ile Asp Gly Thr Gln Ala Met Arg Met Asn Tyr Tyr Pro 
    210                 215                 220                 


Pro Cys Pro Gln Pro Asn Leu Ala Ile Gly Leu Thr Ser His Ser Asp 
225                 230                 235                 240 


Phe Gly Gly Leu Thr Ile Leu Leu Gln Ile Asn Glu Val Glu Gly Leu 
                245                 250                 255     


Gln Ile Lys Arg Glu Gly Thr Trp Ile Ser Val Lys Pro Leu Pro Asn 
            260                 265                 270         


Ala Phe Val Val Asn Val Gly Asp Ile Leu Glu Ile Met Thr Asn Gly 
        275                 280                 285             


Ile Tyr His Ser Val Asp His Arg Ala Val Val Asn Ser Thr Asn Glu 
    290                 295                 300                 


Arg Leu Ser Ile Ala Thr Phe His Asp Pro Ser Leu Glu Ser Val Ile 
305                 310                 315                 320 


Gly Pro Ile Ser Ser Leu Ile Thr Pro Glu Thr Pro Ala Leu Phe Lys 
                325                 330                 335     


Ser Gly Ser Thr Tyr Gly Asp Leu Val Glu Glu Cys Lys Thr Arg Lys 
            340                 345                 350         


Leu Asp Gly Lys Ser Phe Leu Asp Ser Met Arg Ile 
        355                 360                 


<210>  2
<211>  1386
<212>  DNA
<213>  Papaver somniferum

<400>  2
gttcttaatt cattaattaa tttagaaaaa tcatggagaa agcaaaactt atgaagctag       60

gtaatggtat ggaaatacca agtgttcaag aattggctaa actcacgctt gccgaaattc      120

catctcgata cgtatgcgcc aatgaaaacc ttttgttgcc tatgggtgca tctgtcataa      180

atgatcatga aaccattcct gtcatcgata tagaaaattt attatctcca gaaccaataa      240

tcggaaagtt agaattagat aggcttcatt ttgcttgcaa agaatggggt ttttttcagg      300

tagtgaacca tggagtcgac gcttcattgg tggatagtgt aaaatcagaa attcaaggtt      360

tctttaacct ttctatggat gagaaaacta aatatgaaca ggaagatgga gatgtggaag      420

gatttggaca aggctttatt gaatcagagg accaaacact tgattgggca gatatattta      480

tgatgttcac tcttccactc catttaagga agcctcactt attttcaaaa ctcccagtgc      540

ctctcaggga gacaatcgaa tcctactcat cagaaatgaa aaagttatcc atggttctct      600

ttaataagat ggaaaaagct ctacaagtac aagcagccga gattaagggt atgtcagagg      660

tgtttataga tgggacacaa gcaatgagga tgaactatta tcccccttgt cctcaaccaa      720

atctcgccat cggtcttacg tcgcactcgg attttggcgg tttgacaatc ctccttcaaa      780

tcaacgaagt ggaaggatta cagataaaaa gagaggggac atggatttca gtcaaacctc      840

tacctaatgc gttcgtagtg aatgttggag atattttgga gataatgact aatggaattt      900

accatagtgt cgatcaccgg gcagtagtaa actcaacaaa tgagaggctc tcaatcgcaa      960

catttcatga ccctagtcta gagtcggtaa taggcccaat atcaagcttg attactccag     1020

agacacctgc tttgtttaaa agtggatcta catatgggga tcttgtggag gaatgtaaaa     1080

caaggaagct cgatggaaaa tcatttcttg actccatgag gatttgaaaa ctcaagaaaa     1140

aataatacga cgtgattgca tgtcagattc aactatcctt ttgtcgtttt ttggtgctcg     1200

agtccttaat tgttttgatc attgcttttg attctaatta ataagacttt tctcaagaac     1260

cacatgtaat gtacctttac tttcagaaaa taaaaagtat tgaggcacaa atgagaaaat     1320

tgagagagtg cttgagaagt gtaatttctc gaaagtgcgt tgtgtttgaa aaaaaaaaaa     1380

aaaaaa                                                                1386


<210>  3
<211>  360
<212>  PRT
<213>  Papaver somniferum

<400>  3

Met Glu Thr Pro Ile Leu Ile Lys Leu Gly Asn Gly Leu Ser Ile Pro 
1               5                   10                  15      


Ser Val Gln Glu Leu Ala Lys Leu Thr Leu Ala Glu Ile Pro Ser Arg 
            20                  25                  30          


Tyr Thr Cys Thr Gly Glu Ser Pro Leu Asn Asn Ile Gly Ala Ser Val 
        35                  40                  45              


Thr Asp Asp Glu Thr Val Pro Val Ile Asp Leu Gln Asn Leu Leu Ser 
    50                  55                  60                  


Pro Glu Pro Val Val Gly Lys Leu Glu Leu Asp Lys Leu His Ser Ala 
65                  70                  75                  80  


Cys Lys Glu Trp Gly Phe Phe Gln Leu Val Asn His Gly Val Asp Ala 
                85                  90                  95      


Leu Leu Met Asp Asn Ile Lys Ser Glu Ile Lys Gly Phe Phe Asn Leu 
            100                 105                 110         


Pro Met Asn Glu Lys Thr Lys Tyr Gly Gln Gln Asp Gly Asp Phe Glu 
        115                 120                 125             


Gly Phe Gly Gln Pro Tyr Ile Glu Ser Glu Asp Gln Arg Leu Asp Trp 
    130                 135                 140                 


Thr Glu Val Phe Ser Met Leu Ser Leu Pro Leu His Leu Arg Lys Pro 
145                 150                 155                 160 


His Leu Phe Pro Glu Leu Pro Leu Pro Phe Arg Glu Thr Leu Glu Ser 
                165                 170                 175     


Tyr Leu Ser Lys Met Lys Lys Leu Ser Thr Val Val Phe Glu Met Leu 
            180                 185                 190         


Glu Lys Ser Leu Gln Leu Val Glu Ile Lys Gly Met Thr Asp Leu Phe 
        195                 200                 205             


Glu Asp Gly Leu Gln Thr Met Arg Met Asn Tyr Tyr Pro Pro Cys Pro 
    210                 215                 220                 


Arg Pro Glu Leu Val Leu Gly Leu Thr Ser His Ser Asp Phe Ser Gly 
225                 230                 235                 240 


Leu Thr Ile Leu Leu Gln Leu Asn Glu Val Glu Gly Leu Gln Ile Arg 
                245                 250                 255     


Lys Glu Glu Arg Trp Ile Ser Ile Lys Pro Leu Pro Asp Ala Phe Ile 
            260                 265                 270         


Val Asn Val Gly Asp Ile Leu Glu Ile Met Thr Asn Gly Ile Tyr Arg 
        275                 280                 285             


Ser Val Glu His Arg Ala Val Val Asn Ser Thr Lys Glu Arg Leu Ser 
    290                 295                 300                 


Ile Ala Thr Phe His Asp Ser Lys Leu Glu Ser Glu Ile Gly Pro Ile 
305                 310                 315                 320 


Ser Ser Leu Val Thr Pro Glu Thr Pro Ala Leu Phe Lys Arg Gly Arg 
                325                 330                 335     


Tyr Glu Asp Ile Leu Lys Glu Asn Leu Ser Arg Lys Leu Asp Gly Lys 
            340                 345                 350         


Ser Phe Leu Asp Tyr Met Arg Met 
        355                 360 


<210>  4
<211>  1452
<212>  DNA
<213>  Papaver somniferum

<400>  4
gtaaagattg atatatgatc tgaagatctg acaagaaagt tcatcaaata tagagttcat       60

ggagacacca atacttatca agctaggcaa tggtttgtca ataccaagtg ttcaggaatt      120

ggctaaactc acgcttgcag aaattccatc tcgatacaca tgcaccggtg aaagcccgtt      180

gaataatatt ggtgcgtctg taacagatga tgaaacagtt cctgtcatcg atttgcaaaa      240

tttactatct ccagaacccg tagttggaaa gttagaattg gataagcttc attctgcttg      300

caaagaatgg ggtttctttc agctggttaa ccatggagtc gacgctttac tgatggacaa      360

tataaaatca gaaattaaag gtttctttaa ccttccaatg aatgagaaaa ctaaatacgg      420

acagcaagat ggagattttg aaggatttgg acaaccctat attgaatcgg aggaccaaag      480

acttgattgg actgaagtgt ttagcatgtt aagtcttcct ctccatttaa ggaagcctca      540

tttgtttcca gaactccctc tgcctttcag ggagacactg gaatcctacc tatcaaaaat      600

gaaaaaacta tcaacggttg tctttgagat gttggaaaaa tctctacaat tagttgagat      660

taaaggtatg acagacttat ttgaagatgg gttgcaaaca atgaggatga actattatcc      720

tccttgtcct cgaccagagc ttgtacttgg tcttacgtca cactcggatt ttagcggttt      780

gacaattctc cttcaactta atgaagttga aggattacaa ataagaaaag aagagaggtg      840

gatttcaatc aaacctctac ctgatgcgtt catagtgaat gttggagaca ttttggagat      900

aatgactaat gggatttacc gtagcgtcga gcaccgggca gtagtaaact caacaaagga      960

gaggctctca atcgcgacat ttcatgactc taaactagag tcagaaatag gcccaatttc     1020

gagcttggtc acaccagaga cacctgcttt gttcaaaaga ggtaggtatg aggatatttt     1080

gaaggaaaat ctttcaagga agcttgatgg aaaatcattt ctcgactaca tgaggatgtg     1140

agaaagtgtg aacatatatt atactccaca ttgtgtttaa tatatgatga aataagttgc     1200

ttttgaagta tgatgaaata agttggtttt gaagaattca tattgtgctt aaatttcgtg     1260

gatgactgag agatttatta tgtaataata atgtattggt ttgaagattc tcgtctcact     1320

atatgtaaga ctctgtttgg gtcaagtgat gtaatcacgg ttgaaataag ttgcttttga     1380

agaattcata tggtgcttaa tattatgtaa taaataatgt attggattga aaaaaaaaaa     1440

aaaaaaaaaa aa                                                         1452


<210>  5
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  5
gcgcggatcc atggagaaag caaaactt                                          28


<210>  6
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  6
gcgcctgcag cacaacgcac tttcgaga                                          28


<210>  7
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  7
gcgcggatcc atggagacag caaaactt                                          28


<210>  8
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  8
gcgcctgcag agagtcaaaa agcaatga                                          28


<210>  9
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  9
gcgcggatcc atggagacac caatactt                                          28


<210>  10
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  10
gcgcctgcag gcaccatatg aattcttc                                          28


<210>  11
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  11
gcgcggatcc ccttgtcctc aaccaaat                                          28


<210>  12
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  12
gcgcctcgag tccactttta aacaaagc                                          28


<210>  13
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  13
gcgcggatcc cgacgtgatt gcatgtca                                          28


<210>  14
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  14
gcgcctcgag cacaacgcac tttcgaga                                          28


<210>  15
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  15
gcgcggatcc ttgattcgat gaggatg                                           27


<210>  16
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  16
gcgcctcgag cttgagaaaa gttttatt                                          28


<210>  17
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  17
gcgcggatcc gtgagaaagt gtgaacat                                          28


<210>  18
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  18
gcgcctcgag caatccaata cattattt                                          28


<210>  19
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  19
ttgaggcaca aatgagaaaa ttga                                              24


<210>  20
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  20
cacaacgcac tttcgagaaa ttac                                              24


<210>  21
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  21
tgtgagaaac tgaagaacac ccaat                                             25


<210>  22
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  22
aaggactcaa accactgaaa gacg                                              24


<210>  23
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  23
ttgtgcttaa atttcgtgga tgac                                              24


<210>  24
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  24
tgattacatc acttgaccca aacag                                             25


<210>  25
<211>  1326
<212>  DNA
<213>  Papaver somniferum

<400>  25
gaattcggca cgaggcaaat tctatatttc tgataagaaa ctgattaatt attaatttag       60

agaaatcatg gagacagcaa aacttatgaa gctgggtaat ggcatgtcaa taccaagtgt      120

tcaagaattg gctaaactca cgcttgcaga aattccatct cgatacatat gcaccgtaga      180

aaaccttcag ttacctgtgg gtgcatctgt aatagatgat catgaaacag ttcctgtcat      240

tgatatagaa aatttaatat cttcagaacc ggtaaccgaa aagttagaat tggataggct      300

tcattctgct tgcaaagaat ggggtttctt tcaggtggtg aaccatggag tcgatacttc      360

attggtggat aatgtaaaat cagatatcca aggtttcttt aatctttcaa tgaatgagaa      420

aattaaatat ggacagaaag atggagatgt tgaaggattt ggacaagcct ttgttgcatc      480

agaggaccaa acacttgatt gggcagacat ttttatgatc ctcactcttc ctctccattt      540

aaggaagcct catttatttt caaaactccc tctgcctctc agggagacaa tcgaatccta      600

ctcatcagaa atgaaaaaat tatccatggt tctctttgag aagatggaaa aagctctaca      660

agtacaagca gttgagatta aggaaatatc agaggtgttt aaagatatga ctcaagtaat      720

gaggatgaac tattatccac cttgtcctca accagagctc gccatcggtc ttacgccgca      780

ctcagatttt ggcggtttaa caatcctcct tcaactcaac gaagtggaag gattacagat      840

aaaaaacgaa gggagatgga tttcagtcaa acctctacct aatgcgttcg tagtgaatgt      900

tggagatgtt ttggagataa tgactaatgg aatgtaccgt agcgtcgatc atcgggcagt      960

agtaaactca acaaaggaga ggctctcaat cgcaacattt catgacccta atctagagtc     1020

ggaaataggc ccaatatcga gcttgatcac tccaaataca cctgctttgt tcagaagtgg     1080

atctacgtat ggggaacttg tggaggaatt tcattcaagg aagctcgatg gaaaatcatt     1140

tcttgattcg atgaggatgt gagaaactga agaacacaca ataatattac gtgattgtat     1200

gcttgtgtca actgtccttt ccccgtcttt cagtggtctg agtccttatt tgttttcatc     1260

attgcttttt gactctaata aaacttttct caagaaaaaa aaaaaaaaaa aaaaaaaaaa     1320

aaaaaa                                                                1326


<210>  26
<211>  364
<212>  PRT
<213>  Papaver somniferum

<400>  26

Met Glu Thr Ala Lys Leu Met Lys Leu Gly Asn Gly Met Ser Ile Pro 
1               5                   10                  15      


Ser Val Gln Glu Leu Ala Lys Leu Thr Leu Ala Glu Ile Pro Ser Arg 
            20                  25                  30          


Tyr Ile Cys Thr Val Glu Asn Leu Gln Leu Pro Val Gly Ala Ser Val 
        35                  40                  45              


Ile Asp Asp His Glu Thr Val Pro Val Ile Asp Ile Glu Asn Leu Ile 
    50                  55                  60                  


Ser Ser Glu Pro Val Thr Glu Lys Leu Glu Leu Asp Arg Leu His Ser 
65                  70                  75                  80  


Ala Cys Lys Glu Trp Gly Phe Phe Gln Val Val Asn His Gly Val Asp 
                85                  90                  95      


Thr Ser Leu Val Asp Asn Val Lys Ser Asp Ile Gln Gly Phe Phe Asn 
            100                 105                 110         


Leu Ser Met Asn Glu Lys Ile Lys Tyr Gly Gln Lys Asp Gly Asp Val 
        115                 120                 125             


Glu Gly Phe Gly Gln Ala Phe Val Ala Ser Glu Asp Gln Thr Leu Asp 
    130                 135                 140                 


Trp Ala Asp Ile Phe Met Ile Leu Thr Leu Pro Leu His Leu Arg Lys 
145                 150                 155                 160 


Pro His Leu Phe Ser Lys Leu Pro Leu Pro Leu Arg Glu Thr Ile Glu 
                165                 170                 175     


Ser Tyr Ser Ser Glu Met Lys Lys Leu Ser Met Val Leu Phe Glu Lys 
            180                 185                 190         


Met Glu Lys Ala Leu Gln Val Gln Ala Val Glu Ile Lys Glu Ile Ser 
        195                 200                 205             


Glu Val Phe Lys Asp Met Thr Gln Val Met Arg Met Asn Tyr Tyr Pro 
    210                 215                 220                 


Pro Cys Pro Gln Pro Glu Leu Ala Ile Gly Leu Thr Pro His Ser Asp 
225                 230                 235                 240 


Phe Gly Gly Leu Thr Ile Leu Leu Gln Leu Asn Glu Val Glu Gly Leu 
                245                 250                 255     


Gln Ile Lys Asn Glu Gly Arg Trp Ile Ser Val Lys Pro Leu Pro Asn 
            260                 265                 270         


Ala Phe Val Val Asn Val Gly Asp Val Leu Glu Ile Met Thr Asn Gly 
        275                 280                 285             


Met Tyr Arg Ser Val Asp His Arg Ala Val Val Asn Ser Thr Lys Glu 
    290                 295                 300                 


Arg Leu Ser Ile Ala Thr Phe His Asp Pro Asn Leu Glu Ser Glu Ile 
305                 310                 315                 320 


Gly Pro Ile Ser Ser Leu Ile Thr Pro Asn Thr Pro Ala Leu Phe Arg 
                325                 330                 335     


Ser Gly Phe Thr Tyr Gly Glu Leu Val Glu Glu Phe His Ser Arg Lys 
            340                 345                 350         


Leu Asp Gly Lys Ser Phe Phe Asp Ser Met Arg Met 
        355                 360                 


