                         SEQUENCE LISTING

<110>  Alexion Pharmaceuticals, Inc.
 
<120>  REAGENTS AND METHODS FOR DETECTING PNH TYPE II CELLS 

<130>  ALXN-150-WO1

<140> NOT YET ASSIGNED
<141> 2010-11-09

<150> 61/280,897
<151> 2009-11-09

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  492
<212>  PRT
<213>  Aeromonas hydrophila

<400>  1

Gln Lys Ile Lys Leu Thr Gly Leu Ser Leu Ile Ile Ser Gly Leu Leu 
1               5                   10                  15      


Met Ala Gln Ala Gln Ala Ala Glu Pro Val Tyr Pro Asp Gln Leu Arg 
            20                  25                  30          


Leu Phe Ser Leu Gly Gln Gly Val Cys Gly Asp Lys Tyr Arg Pro Val 
        35                  40                  45              


Asn Arg Glu Glu Ala Gln Ser Val Lys Ser Asn Ile Val Gly Met Met 
    50                  55                  60                  


Gly Gln Trp Gln Ile Ser Gly Leu Ala Asn Gly Trp Val Ile Met Gly 
65                  70                  75                  80  


Pro Gly Tyr Asn Gly Glu Ile Lys Pro Gly Thr Ala Ser Asn Thr Trp 
                85                  90                  95      


Cys Tyr Pro Thr Asn Pro Val Thr Gly Glu Ile Pro Thr Leu Ser Ala 
            100                 105                 110         


Leu Asp Ile Pro Asp Gly Asp Glu Val Asp Val Gln Trp Arg Leu Val 
        115                 120                 125             


His Asp Ser Ala Asn Phe Ile Lys Pro Thr Ser Tyr Leu Ala His Tyr 
    130                 135                 140                 



Leu Gly Tyr Ala Trp Val Gly Gly Asn His Ser Gln Tyr Val Gly Glu 
145                 150                 155                 160 


Asp Met Asp Val Thr Arg Asp Gly Asp Gly Trp Val Ile Arg Gly Asn 
                165                 170                 175     


Asn Asp Gly Gly Cys Asp Gly Tyr Arg Cys Gly Asp Lys Thr Ala Ile 
            180                 185                 190         


Lys Val Ser Asn Phe Ala Tyr Asn Leu Asp Pro Asp Ser Phe Lys His 
        195                 200                 205             


Gly Asp Val Thr Gln Ser Asp Arg Gln Leu Val Lys Thr Val Val Gly 
    210                 215                 220                 


Trp Ala Val Asn Asp Ser Asp Thr Pro Gln Ser Gly Tyr Asp Val Thr 
225                 230                 235                 240 


Leu Arg Tyr Asp Thr Ala Thr Asn Trp Ser Lys Thr Asn Thr Tyr Gly 
                245                 250                 255     


Leu Ser Glu Lys Val Thr Thr Lys Asn Lys Phe Lys Trp Pro Leu Val 
            260                 265                 270         


Gly Glu Thr Glu Leu Ser Ile Glu Ile Ala Ala Asn Gln Ser Trp Ala 
        275                 280                 285             


Ser Gln Asn Gly Gly Ser Thr Thr Thr Ser Leu Ser Gln Ser Val Arg 
    290                 295                 300                 


Pro Thr Val Pro Ala Arg Ser Lys Ile Pro Val Lys Ile Glu Leu Tyr 
305                 310                 315                 320 


Lys Ala Asp Ile Ser Tyr Pro Tyr Glu Phe Lys Ala Asp Val Ser Tyr 
                325                 330                 335     


Asp Leu Thr Leu Ser Gly Phe Leu Arg Trp Gly Gly Asn Ala Trp Tyr 
            340                 345                 350         


Thr His Pro Asp Asn Arg Pro Asn Trp Asn His Thr Phe Val Ile Gly 
        355                 360                 365             



Pro Tyr Lys Asp Lys Ala Ser Ser Ile Arg Tyr Gln Trp Asp Lys Arg 
    370                 375                 380                 


Tyr Ile Pro Gly Glu Val Lys Trp Trp Asp Trp Asn Trp Thr Ile Gln 
385                 390                 395                 400 


Gln Asn Gly Leu Ser Thr Met Gln Asn Asn Leu Ala Arg Val Leu Arg 
                405                 410                 415     


Pro Val Arg Ala Gly Ile Thr Gly Asp Phe Ser Ala Glu Ser Gln Phe 
            420                 425                 430         


Ala Gly Asn Ile Glu Ile Gly Ala Pro Val Pro Leu Ala Ala Asp Ser 
        435                 440                 445             


His Ser Ser Lys Leu Gln Ser Val Asp Gly Ala Gly Gln Gly Leu Arg 
    450                 455                 460                 


Leu Glu Ile Pro Leu Asp Ala Gln Glu Leu Ser Gly Leu Gly Phe Asn 
465                 470                 475                 480 


Asn Val Ser Leu Ser Val Thr Pro Ala Ala Asn Gln 
                485                 490         


<210>  2
<211>  470
<212>  PRT
<213>  Aeromonas hydrophila

<400>  2

Ala Glu Pro Val Tyr Pro Asp Gln Leu Arg Leu Phe Ser Leu Gly Gln 
1               5                   10                  15      


Gly Val Cys Gly Asp Lys Tyr Arg Pro Val Asn Arg Glu Glu Ala Gln 
            20                  25                  30          


Ser Val Lys Ser Asn Ile Val Gly Met Met Gly Gln Trp Gln Ile Ser 
        35                  40                  45              


Gly Leu Ala Asn Gly Trp Val Ile Met Gly Pro Gly Tyr Asn Gly Glu 
    50                  55                  60                  




Ile Lys Pro Gly Thr Ala Ser Asn Thr Trp Cys Tyr Pro Thr Asn Pro 
65                  70                  75                  80  


Val Thr Gly Glu Ile Pro Thr Leu Ser Ala Leu Asp Ile Pro Asp Gly 
                85                  90                  95      


Asp Glu Val Asp Val Gln Trp Arg Leu Val His Asp Ser Ala Asn Phe 
            100                 105                 110         


Ile Lys Pro Thr Ser Tyr Leu Ala His Tyr Leu Gly Tyr Ala Trp Val 
        115                 120                 125             


Gly Gly Asn His Ser Gln Tyr Val Gly Glu Asp Met Asp Val Thr Arg 
    130                 135                 140                 


Asp Gly Asp Gly Trp Val Ile Arg Gly Asn Asn Asp Gly Gly Cys Asp 
145                 150                 155                 160 


Gly Tyr Arg Cys Gly Asp Lys Thr Ala Ile Lys Val Ser Asn Phe Ala 
                165                 170                 175     


Tyr Asn Leu Asp Pro Asp Ser Phe Lys His Gly Asp Val Thr Gln Ser 
            180                 185                 190         


Asp Arg Gln Leu Val Lys Thr Val Val Gly Trp Ala Val Asn Asp Ser 
        195                 200                 205             


Asp Thr Pro Gln Ser Gly Tyr Asp Val Thr Leu Arg Tyr Asp Thr Ala 
    210                 215                 220                 


Thr Asn Trp Ser Lys Thr Asn Thr Tyr Gly Leu Ser Glu Lys Val Thr 
225                 230                 235                 240 


Thr Lys Asn Lys Phe Lys Trp Pro Leu Val Gly Glu Thr Glu Leu Ser 
                245                 250                 255     


Ile Glu Ile Ala Ala Asn Gln Ser Trp Ala Ser Gln Asn Gly Gly Ser 
            260                 265                 270         


Thr Thr Thr Ser Leu Ser Gln Ser Val Arg Pro Thr Val Pro Ala Arg 
        275                 280                 285             



Ser Lys Ile Pro Val Lys Ile Glu Leu Tyr Lys Ala Asp Ile Ser Tyr 
    290                 295                 300                 


Pro Tyr Glu Phe Lys Ala Asp Val Ser Tyr Asp Leu Thr Leu Ser Gly 
305                 310                 315                 320 


Phe Leu Arg Trp Gly Gly Asn Ala Trp Tyr Thr His Pro Asp Asn Arg 
                325                 330                 335     


Pro Asn Trp Asn His Thr Phe Val Ile Gly Pro Tyr Lys Asp Lys Ala 
            340                 345                 350         


Ser Ser Ile Arg Tyr Gln Trp Asp Lys Arg Tyr Ile Pro Gly Glu Val 
        355                 360                 365             


Lys Trp Trp Asp Trp Asn Trp Thr Ile Gln Gln Asn Gly Leu Ser Thr 
    370                 375                 380                 


Met Gln Asn Asn Leu Ala Arg Val Leu Arg Pro Val Arg Ala Gly Ile 
385                 390                 395                 400 


Thr Gly Asp Phe Ser Ala Glu Ser Gln Phe Ala Gly Asn Ile Glu Ile 
                405                 410                 415     


Gly Ala Pro Val Pro Leu Ala Ala Asp Ser His Ser Ser Lys Leu Gln 
            420                 425                 430         


Ser Val Asp Gly Ala Gly Gln Gly Leu Arg Leu Glu Ile Pro Leu Asp 
        435                 440                 445             


Ala Gln Glu Leu Ser Gly Leu Gly Phe Asn Asn Val Ser Leu Ser Val 
    450                 455                 460                 


Thr Pro Ala Ala Asn Gln 
465                 470 











<210>  3
<211>  439
<212>  PRT
<213>  Aeromonas hydrophila

<400>  3

Ala Glu Pro Val Tyr Pro Asp Gln Leu Arg Leu Phe Ser Leu Gly Gln 
1               5                   10                  15      


Gly Val Cys Gly Asp Lys Tyr Arg Pro Val Asn Arg Glu Glu Ala Gln 
            20                  25                  30          


Ser Val Lys Ser Asn Ile Val Gly Met Met Gly Gln Trp Gln Ile Ser 
        35                  40                  45              


Gly Leu Ala Asn Gly Trp Val Ile Met Gly Pro Gly Tyr Asn Gly Glu 
    50                  55                  60                  


Ile Lys Pro Gly Thr Ala Ser Asn Thr Trp Cys Tyr Pro Thr Asn Pro 
65                  70                  75                  80  


Val Thr Gly Glu Ile Pro Thr Leu Ser Ala Leu Asp Ile Pro Asp Gly 
                85                  90                  95      


Asp Glu Val Asp Val Gln Trp Arg Leu Val His Asp Ser Ala Asn Phe 
            100                 105                 110         


Ile Lys Pro Thr Ser Tyr Leu Ala His Tyr Leu Gly Tyr Ala Trp Val 
        115                 120                 125             


Gly Gly Asn His Ser Gln Tyr Val Gly Glu Asp Met Asp Val Thr Arg 
    130                 135                 140                 


Asp Gly Asp Gly Trp Val Ile Arg Gly Asn Asn Asp Gly Gly Cys Asp 
145                 150                 155                 160 


Gly Tyr Arg Cys Gly Asp Lys Thr Ala Ile Lys Val Ser Asn Phe Ala 
                165                 170                 175     


Tyr Asn Leu Asp Pro Asp Ser Phe Lys His Gly Asp Val Thr Gln Ser 
            180                 185                 190         


Asp Arg Gln Leu Val Lys Thr Val Val Gly Trp Ala Val Asn Asp Ser 
        195                 200                 205             


Asp Thr Pro Gln Ser Gly Tyr Asp Val Thr Leu Arg Tyr Asp Thr Ala 
    210                 215                 220                 


Thr Asn Trp Ser Lys Thr Asn Thr Tyr Gly Leu Ser Glu Lys Val Thr 
225                 230                 235                 240 


Thr Lys Asn Lys Phe Lys Trp Pro Leu Val Gly Glu Thr Glu Leu Ser 
                245                 250                 255     


Ile Glu Ile Ala Ala Asn Gln Ser Trp Ala Ser Gln Asn Gly Gly Ser 
            260                 265                 270         


Thr Thr Thr Ser Leu Ser Gln Ser Val Arg Pro Thr Val Pro Ala Arg 
        275                 280                 285             


Ser Lys Ile Pro Val Lys Ile Glu Leu Tyr Lys Ala Asp Ile Ser Tyr 
    290                 295                 300                 


Pro Tyr Glu Phe Lys Ala Asp Val Ser Tyr Asp Leu Thr Leu Ser Gly 
305                 310                 315                 320 


Phe Leu Arg Trp Gly Gly Asn Ala Trp Tyr Thr His Pro Asp Asn Arg 
                325                 330                 335     


Pro Asn Trp Asn His Thr Phe Val Ile Gly Pro Tyr Lys Asp Lys Ala 
            340                 345                 350         


Ser Ser Ile Arg Tyr Gln Trp Asp Lys Arg Tyr Ile Pro Gly Glu Val 
        355                 360                 365             


Lys Trp Trp Asp Trp Asn Trp Thr Ile Gln Gln Asn Gly Leu Ser Thr 
    370                 375                 380                 


Met Gln Asn Asn Leu Ala Arg Val Leu Arg Pro Val Arg Ala Gly Ile 
385                 390                 395                 400 


Thr Gly Asp Phe Ser Ala Glu Ser Gln Phe Ala Gly Asn Ile Glu Ile 
                405                 410                 415     


Gly Ala Pro Val Pro Leu Ala Ala Asp Ser His Ser Ser Lys Leu Gln 
            420                 425                 430         


Ser Val Asp Gly Ala Gly Gln 
        435                 


<210>  4
<211>  128
<212>  PRT
<213>  Aeromonas hydrophila

<400>  4

Leu Asp Pro Asp Ser Phe Lys His Gly Asp Val Thr Gln Ser Asp Arg 
1               5                   10                  15      


Gln Leu Val Lys Thr Val Val Gly Trp Ala Val Asn Asp Ser Asp Thr 
            20                  25                  30          


Pro Gln Ser Gly Tyr Asp Val Thr Leu Arg Tyr Asp Thr Ala Thr Asn 
        35                  40                  45              


Trp Ser Lys Thr Asn Thr Tyr Gly Leu Ser Glu Lys Val Thr Thr Lys 
    50                  55                  60                  


Asn Lys Phe Lys Trp Pro Leu Val Gly Glu Thr Glu Leu Ser Ile Glu 
65                  70                  75                  80  


Ile Ala Ala Asn Gln Ser Trp Ala Ser Gln Asn Gly Gly Ser Thr Thr 
                85                  90                  95      


Thr Ser Leu Ser Gln Ser Val Arg Pro Thr Val Pro Ala Arg Ser Lys 
            100                 105                 110         


Ile Pro Val Lys Ile Glu Leu Tyr Lys Ala Asp Ile Ser Tyr Pro Tyr 
        115                 120                 125             


<210>  5
<211>  128
<212>  PRT
<213>  Aeromonas hydrophila

<400>  5

Leu Asp Pro Asp Ser Phe Lys His Gly Asp Val Thr Gln Ser Asp Arg 
1               5                   10                  15      


Gln Leu Val Lys Thr Val Val Gly Trp Ala Val Asn Asp Ser Asp Thr 
            20                  25                  30          


Pro Gln Ser Gly Tyr Asp Val Thr Leu Arg Tyr Asp Thr Ala Thr Asn 
        35                  40                  45              


Trp Ser Lys Thr Asn Thr Tyr Gly Leu Ser Glu Lys Val Thr Thr Lys 
    50                  55                  60                  


Asn Lys Phe Lys Trp Pro Leu Val Gly Glu Cys Glu Leu Ser Ile Glu 
65                  70                  75                  80  


Ile Ala Ala Asn Gln Ser Trp Ala Ser Gln Asn Gly Gly Ser Thr Thr 
                85                  90                  95      


Thr Ser Leu Ser Gln Ser Val Arg Pro Thr Val Pro Ala Arg Ser Lys 
            100                 105                 110         


Ile Pro Val Lys Ile Glu Leu Tyr Lys Cys Asp Ile Ser Tyr Pro Tyr 
        115                 120                 125             


<210>  6
<211>  492
<212>  PRT
<213>  Aeromonas salmonicida

<400>  6

Met Lys Lys Leu Lys Ile Thr Gly Leu Ser Leu Ile Ile Ser Gly Leu 
1               5                   10                  15      


Leu Met Ala Gln Ala Gln Ala Ala Glu Pro Val Tyr Pro Asp Gln Leu 
            20                  25                  30          


Arg Leu Phe Ser Leu Gly Gln Glu Val Cys Gly Asp Lys Tyr Arg Pro 
        35                  40                  45              


Val Asn Arg Glu Glu Ala Gln Ser Val Lys Ser Asn Ile Val Gly Met 
    50                  55                  60                  


Met Gly Gln Trp Gln Ile Ser Gly Leu Ala Asn Gly Trp Val Ile Met 
65                  70                  75                  80  


Gly Pro Gly Tyr Asn Gly Glu Ile Lys Pro Gly Ser Ala Ser Ser Thr 
                85                  90                  95      


Trp Cys Tyr Pro Thr Asn Pro Ala Thr Gly Glu Ile Pro Thr Leu Ser 
            100                 105                 110         


Ala Leu Asp Ile Pro Asp Gly Asp Glu Val Asp Val Gln Trp Arg Leu 
        115                 120                 125             


Val His Asp Ser Ala Asn Phe Ile Lys Pro Thr Ser Tyr Leu Ala His 
    130                 135                 140                 


Tyr Leu Gly Tyr Ala Trp Val Gly Gly Asn His Ser Gln Tyr Val Gly 
145                 150                 155                 160 


Glu Asp Met Asp Val Thr Arg Asp Gly Asp Gly Trp Val Ile Arg Gly 
                165                 170                 175     


Asn Asn Asp Gly Gly Cys Asp Gly Tyr Arg Cys Gly Asp Lys Thr Ser 
            180                 185                 190         


Ile Lys Val Ser Asn Phe Ala Tyr Asn Leu Asp Pro Asp Ser Phe Lys 
        195                 200                 205             


His Gly Asp Val Thr Gln Ser Asp Arg Gln Leu Val Lys Thr Val Val 
    210                 215                 220                 


Gly Trp Ala Ile Asn Asp Ser Asp Thr Pro Gln Ser Gly Tyr Asp Val 
225                 230                 235                 240 


Thr Leu Arg Tyr Asp Thr Ala Thr Asn Trp Ser Lys Thr Asn Thr Tyr 
                245                 250                 255     


Gly Leu Ser Glu Lys Val Thr Thr Lys Asn Lys Phe Lys Trp Pro Leu 
            260                 265                 270         


Val Gly Glu Thr Glu Leu Ser Ile Glu Ile Ala Ala Asn Gln Ser Trp 
        275                 280                 285             


Ala Ser Gln Asn Gly Gly Ser Thr Thr Thr Ser Leu Ser Gln Ser Val 
    290                 295                 300                 


Arg Pro Thr Val Pro Ala His Ser Lys Ile Pro Val Lys Ile Glu Leu 
305                 310                 315                 320 



Tyr Lys Ala Asp Ile Ser Tyr Pro Tyr Glu Phe Lys Ala Asp Val Ser 
                325                 330                 335     


Tyr Asp Leu Thr Leu Ser Gly Phe Leu Arg Trp Gly Gly Asn Ala Trp 
            340                 345                 350         


Tyr Thr His Pro Asp Asn Arg Pro Asn Trp Asn His Thr Phe Val Ile 
        355                 360                 365             


Gly Pro Tyr Lys Asp Lys Ala Ser Ser Ile Arg Tyr Gln Trp Asp Lys 
    370                 375                 380                 


Arg Tyr Ile Pro Gly Glu Val Lys Trp Ser Asp Trp Asn Trp Thr Ile 
385                 390                 395                 400 


Gln Gln Asn Gly Leu Ser Thr Met Gln Asn Asn Leu Ala Arg Val Leu 
                405                 410                 415     


Arg Pro Val Arg Ala Gly Ile Thr Gly Asp Phe Ser Ala Glu Ser Gln 
            420                 425                 430         


Phe Ala Gly Asn Ile Glu Ile Gly Ala Pro Val Pro Val Ala Ala Ala 
        435                 440                 445             


Ser Gln Ser Ser Arg Ala Arg Asn Leu Ser Ala Gly Gln Gly Leu Arg 
    450                 455                 460                 


Leu Glu Ile Pro Leu Asp Ala Gln Glu Leu Ser Gly Leu Gly Phe Asn 
465                 470                 475                 480 


Asn Val Ser Leu Ser Val Thr Pro Ala Ala Asn Gln 
                485                 490         















<210>  7
<211>  469
<212>  PRT
<213>  Aeromonas salmonicida

<400>  7

Ala Glu Pro Val Tyr Pro Asp Gln Leu Arg Leu Phe Ser Leu Gly Gln 
1               5                   10                  15      


Glu Val Cys Gly Asp Lys Tyr Arg Pro Val Asn Arg Glu Glu Ala Gln 
            20                  25                  30          


Ser Val Lys Ser Asn Ile Val Gly Met Met Gly Gln Trp Gln Ile Ser 
        35                  40                  45              


Gly Leu Ala Asn Gly Trp Val Ile Met Gly Pro Gly Tyr Asn Gly Glu 
    50                  55                  60                  


Ile Lys Pro Gly Ser Ala Ser Ser Thr Trp Cys Tyr Pro Thr Asn Pro 
65                  70                  75                  80  


Ala Thr Gly Glu Ile Pro Thr Leu Ser Ala Leu Asp Ile Pro Asp Gly 
                85                  90                  95      


Asp Glu Val Asp Val Gln Trp Arg Leu Val His Asp Ser Ala Asn Phe 
            100                 105                 110         


Ile Lys Pro Thr Ser Tyr Leu Ala His Tyr Leu Gly Tyr Ala Trp Val 
        115                 120                 125             


Gly Gly Asn His Ser Gln Tyr Val Gly Glu Asp Met Asp Val Thr Arg 
    130                 135                 140                 


Asp Gly Asp Gly Trp Val Ile Arg Gly Asn Asn Asp Gly Gly Cys Asp 
145                 150                 155                 160 


Gly Tyr Arg Cys Gly Asp Lys Thr Ser Ile Lys Val Ser Asn Phe Ala 
                165                 170                 175     


Tyr Asn Leu Asp Pro Asp Ser Phe Lys His Gly Asp Val Thr Gln Ser 
            180                 185                 190         


Asp Arg Gln Leu Val Lys Thr Val Val Gly Trp Ala Ile Asn Asp Ser 
        195                 200                 205             


Asp Thr Pro Gln Ser Gly Tyr Asp Val Thr Leu Arg Tyr Asp Thr Ala 
    210                 215                 220                 


Thr Asn Trp Ser Lys Thr Asn Thr Tyr Gly Leu Ser Glu Lys Val Thr 
225                 230                 235                 240 


Thr Lys Asn Lys Phe Lys Trp Pro Leu Val Gly Glu Thr Glu Leu Ser 
                245                 250                 255     


Ile Glu Ile Ala Ala Asn Gln Ser Trp Ala Ser Gln Asn Gly Gly Ser 
            260                 265                 270         


Thr Thr Thr Ser Leu Ser Gln Ser Val Arg Pro Thr Val Pro Ala His 
        275                 280                 285             


Ser Lys Ile Pro Val Lys Ile Glu Leu Tyr Lys Ala Asp Ile Ser Tyr 
    290                 295                 300                 


Pro Tyr Glu Phe Lys Ala Asp Val Ser Tyr Asp Leu Thr Leu Ser Gly 
305                 310                 315                 320 


Phe Leu Arg Trp Gly Gly Asn Ala Trp Tyr Thr His Pro Asp Asn Arg 
                325                 330                 335     


Pro Asn Trp Asn His Thr Phe Val Ile Gly Pro Tyr Lys Asp Lys Ala 
            340                 345                 350         


Ser Ser Ile Arg Tyr Gln Trp Asp Lys Arg Tyr Ile Pro Gly Glu Val 
        355                 360                 365             


Lys Trp Ser Asp Trp Asn Trp Thr Ile Gln Gln Asn Gly Leu Ser Thr 
    370                 375                 380                 


Met Gln Asn Asn Leu Ala Arg Val Leu Arg Pro Val Arg Ala Gly Ile 
385                 390                 395                 400 


Thr Gly Asp Phe Ser Ala Glu Ser Gln Phe Ala Gly Asn Ile Glu Ile 
                405                 410                 415     


Gly Ala Pro Val Pro Val Ala Ala Ala Ser Gln Ser Ser Arg Ala Arg 
            420                 425                 430         


Asn Leu Ser Ala Gly Gln Gly Leu Arg Leu Glu Ile Pro Leu Asp Ala 
        435                 440                 445             


Gln Glu Leu Ser Gly Leu Gly Phe Asn Asn Val Ser Leu Ser Val Thr 
    450                 455                 460                 


Pro Ala Ala Asn Gln 
465                 







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