                               SEQUENCE LISTING	

<110> THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATE
      AMIDE BIO LLC
 
<120> EFFICIENT PRODUCTION OF PEPTIDES

<130> 39538-702.601

<140>
<141>

<150> 61/259,367
<151> 2009-11-09

<160> 13    

<170> PatentIn version 3.5

<210> 1
<211> 132
<212> PRT
<213> Comamonas testosteroni

<220> 
<223> strain CNB-2

<400> 1
Met Ser Lys Gln Glu Ser Gln Pro Asp Val Gln Phe Leu Ala Arg Phe 
1               5                   10                  15      


Ser Asp Ala Trp Asn Arg His Asp Ile Asp Ala Leu Met Asp Phe Met 
            20                  25                  30          


Ala Asp Glu Cys Glu Phe His Ala Val Ala Gly Pro Asp Leu Met Gly 
        35                  40                  45              


Arg Ser Phe Val Gly Arg Glu Ala Val Arg Glu Gly Phe Gln Leu Ala 
    50                  55                  60                  


Trp Gln Ala Phe Pro Asp Ala Ala Trp Val Asp Gly Glu His Phe Val 
65                  70                  75                  80  


Gln Gly Thr Arg Gly Val Ser Glu Ser Thr Phe Lys Gly Thr Lys Ala 
                85                  90                  95      


Asp Gly Leu Arg Val Glu Ala Arg Met Val Asp Val Phe Thr Phe Arg 
            100                 105                 110         


Asp Gly Lys Ile Ala Val Lys Asn Ala Tyr Arg Lys Asp Arg Pro Pro 
        115                 120                 125             


Val Ala Ile Ser 
    130         


<210> 2
<211> 148
<212> PRT
<213> Legionella pneumophila

<220>
<223> strain Corby; ketosteroid isomerase

<400> 2
Met Lys Ser Ile Ala Asn Lys Glu Asn Glu Val Ala Ile Ile His Lys 
1               5                   10                  15      


Leu Ile Glu Glu Tyr Ala Asn Ala Ala Arg Asp Lys Asp Ile Asp Lys 
            20                  25                  30          


Ile Met Ser His Tyr Ala Pro Asp Ile Arg Ser Phe Asp Ala Tyr Ser 
        35                  40                  45              


Gln Leu Gln Phe Lys Gly Ala Asp Asp Tyr Arg Lys His Trp Gln Thr 
    50                  55                  60                  


Cys Leu Ser Phe Cys Pro Gly Pro Ser Val Phe Glu Val His Gln Leu 
65                  70                  75                  80  


Glu Thr Ile Val Asp Asn Asn Leu Ala Val Ser Tyr Tyr Leu Thr Tyr 
                85                  90                  95      


Cys Gly Gly Thr Asn Glu Lys Gly Glu Thr Gln Gly Gly Trp Met Arg 
            100                 105                 110         


Gly Thr Met Val His Cys Lys Met Asn Gly Lys Trp Lys Ile Met His 
        115                 120                 125             


Glu His Tyr Ser Ile Pro Phe Asp Met Lys Thr Gly Asn Thr Leu Phe 
    130                 135                 140                 


Asp Leu Lys Pro 
145             


<210> 3
<211> 153
<212> PRT
<213> Pseudomonas stutzeri

<220>
<223> strain A1501; ketosteroid isomerase

<400> 3
Met Asn Asn Thr Thr Arg Gln Asp Glu Thr Ala Ile Arg Gln Leu His 
1               5                   10                  15      


Asp Ala Phe Glu Gln Ala Ile Gly Ala Lys Asp Leu Asp Arg Ile Met 
            20                  25                  30          


Ala Gln Tyr Ala Pro Asp Val Val Ala Phe Asp Ala Val Gly Ala Leu 
        35                  40                  45              


Gln Phe Lys Gly Val Ala Glu Tyr Arg Ala His Trp Gln Arg Cys Phe 
    50                  55                  60                  


Glu Phe Cys Gln Gly Glu Gly Phe Phe Glu Thr His Glu Leu His Val 
65                  70                  75                  80  


Asp Val Gly Gly Glu Leu Ala Cys Ser Arg Met Leu Thr His Cys Gly 
                85                  90                  95      


Gly Pro Asn Ala Glu Gly Glu Met Gln Thr Ala Trp Met Arg Gly Thr 
            100                 105                 110         


Arg Val Trp Ala Arg Arg Asp Gly Glu Trp Lys Val Ile His Glu His 
        115                 120                 125             


Phe Ser Met Pro Phe Asp Met Glu Thr Gly Gln Val Cys Met Ser Ser 
    130                 135                 140                 


Thr Pro Ser Ala Thr Gln Gln Val Gly 
145                 150             


<210> 4
<211> 124
<212> PRT
<213> Pseudomonas testosteroni

<220>
<223> delta 5-3-ketosteroid isomerase

<400> 4
His Thr Pro Glu His Ile Thr Ala Val Val Gln Arg Phe Val Ala Ala 
1               5                   10                  15      


Leu Asn Ala Gly Asp Leu Asp Gly Ile Val Ala Leu Phe Ala Asp Asp 
            20                  25                  30          


Ala Thr Val Glu Asp Pro Val Gly Ser Glu Pro Arg Ser Gly Thr Ala 
        35                  40                  45              


Ala Ile Arg Glu Phe Tyr Ala Asn Ser Leu Lys Leu Pro Leu Ala Val 
    50                  55                  60                  


Glu Leu Thr Gln Glu Val Arg Ala Val Ala Asn Glu Ala Ala Phe Ala 
65                  70                  75                  80  


Phe Thr Val Ser Phe Glu Tyr Gln Gly Arg Lys Thr Val Val Ala Pro 
                85                  90                  95      


Ile Asp His Phe Arg Phe Asn Gly Ala Gly Lys Val Val Ser Ile Arg 
            100                 105                 110         


Ala Leu Phe Gly Glu Lys Asn Ile His Ala Cys Gln 
        115                 120                 


<210> 5
<211> 378
<212> DNA
<213> Pseudomonas testosteroni

<220>
<223> ketosteroid isomerase (ksi) gene

<400> 5
cataccccag aacacatcac cgccgtggta cagcgctttg tggctgcgct caatgccggc       60

gatctggacg gcatcgtcgc gctgtttgcc gatgacgcca cggtggaaga ccccgtgggt      120

tccgagccca ggtccggtac ggctgcgatt cgtgagtttt acgccaactc gctcaaactg      180

cctttggcgg tggagctgac gcaggaggta cgcgcggtcg ccaacgaagc ggccttcgct      240

ttcaccgtca gcttcgagta tcagggccgc aagaccgtag ttgcgcccat cgatcacttt      300

cgcttcaatg gcgccggcaa ggtggtgagc atccgcgcct tgtttggcga gaagaatatt      360

cacgcatgcc agctcgag                                                    378


<210> 6
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 6
His His His His His His 
1               5       


<210> 7
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<220>
<221> misc_feature
<222> (1)..(10)
<223> This sequence may encompass 5-10 repeating 'Lys' residues

<400> 7
Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys 
1               5                   10  


<210> 8
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<220>
<221> misc_feature
<222> (1)..(10)
<223> This sequence may encompass 5-10 repeating 'His' residues

<400> 8
His His His His His His His His His His 
1               5                   10  


<210> 9
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<220>
<221> misc_feature
<222> (1)..(10)
<223> This sequence may encompass 5-10 repeating 'Glu' residues

<400> 9
Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu 
1               5                   10  


<210> 10
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<220>
<221> misc_feature
<222> (1)..(10)
<223> This sequence may encompass 5-10 repeating 'Arg' residues

<400> 10
Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg 
1               5                   10  


<210> 11
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 11
Lys Lys Lys Lys Lys Lys 
1               5       


<210> 12
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 12
Glu Glu Glu Glu Glu Glu 
1               5       


<210> 13
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 13
Arg Arg Arg Arg Arg Arg 
1               5       
