
                                SEQUENCE LISTING

<110> Merck Sharp & Dohme Corp.
      Ichetovkin, Marina
      Chen, Zhu
      LeGrand, Cheryl

<120> PCSK9 IMMUNOASSAY
  

<130> MRL-ACV-00030

<150> US 61/368,081       
<151> 2010-07-27  

<150> US 61/256,752       
<151> 2009-10-30  

<160> 24

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 215
<212> PRT
<213> Artificial Sequence

<220> 
<223> AX213 Antibody Full Light Chain

<400> 1
Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly
 1               5                  10                  15      
Glu Arg Ala Thr Ile Thr Cys Arg Ala Ser Gln Tyr Val Gly Ser Tyr
            20                  25                  30          
Leu Asn Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile
        35                  40                  45              
Tyr Asp Ala Ser Asn Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro
65                  70                  75                  80  
Glu Asp Phe Ala Val Tyr Tyr Cys Gln Val Trp Asp Ser Ser Pro Pro
                85                  90                  95      
Val Val Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala
            100                 105                 110         
Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser
        115                 120                 125             
Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu
    130                 135                 140                 
Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser
145                 150                 155                 160 
Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu
                165                 170                 175     
Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val
            180                 185                 190         
Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys
        195                 200                 205             
Ser Phe Asn Arg Gly Glu Cys
    210                 215 


<210> 2
<211> 645
<212> DNA
<213> Artificial Sequence

<220> 
<223> AX213 Antibody Full Light Chain

<400> 2
gaaatcgtgc tgacccagtc tccagccacc ctgtctctgt ctcccgggga acgtgccacc 60
atcacctgcc gtgcctctca gtatgtcggc agctacctga actggtatca gcagaagcca 120
ggtcaggcgc cacgtctgct gatctacgac gcctctaacc gtgccaccgg tatcccagcc 180
cgtttctctg gttctggttc tggcaccgac ttcaccctga ccatctcttc tctggaacca 240
gaagacttcg ccgtgtacta ctgccaggta tgggacagct ctcctcctgt ggtgttcggt 300
ggtggtacca aagtggaaat caagcgtacg gtggctgcac catctgtatt catcttcccg 360
ccatctgatg agcagttgaa atctggaact gcctctgttg tgtgcctgct gaataacttc 420
tatcccagag aggccaaagt acagtggaag gtggataacg ccctccaatc gggtaactcc 480
caggagagtg tcacagagca ggacagcaag gacagcacct acagcctcag cagcaccctg 540
acgctgagca aagcagacta cgagaaacac aaagtctacg cctgcgaagt cacccatcag 600
ggcctgagct cgcccgtcac aaagagcttc aacaggggag agtgt                 645

<210> 3
<211> 108
<212> PRT
<213> Artificial Sequence

<220> 
<223> AX213 Variable Light Antibody Region

<400> 3
Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly
 1               5                  10                  15      
Glu Arg Ala Thr Ile Thr Cys Arg Ala Ser Gln Tyr Val Gly Ser Tyr
            20                  25                  30          
Leu Asn Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile
        35                  40                  45              
Tyr Asp Ala Ser Asn Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro
65                  70                  75                  80  
Glu Asp Phe Ala Val Tyr Tyr Cys Gln Val Trp Asp Ser Ser Pro Pro
                85                  90                  95      
Val Val Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
            100                 105             


<210> 4
<211> 324
<212> DNA
<213> Artificial Sequence

<220> 
<223> AX213 Variable Light Antibody Region

<400> 4
gaaatcgtgc tgacccagtc tccagccacc ctgtctctgt ctcccgggga acgtgccacc 60
atcacctgcc gtgcctctca gtatgtcggc agctacctga actggtatca gcagaagcca 120
ggtcaggcgc cacgtctgct gatctacgac gcctctaacc gtgccaccgg tatcccagcc 180
cgtttctctg gttctggttc tggcaccgac ttcaccctga ccatctcttc tctggaacca 240
gaagacttcg ccgtgtacta ctgccaggta tgggacagct ctcctcctgt ggtgttcggt 300
ggtggtacca aagtggagat caaa                                        324

<210> 5
<211> 226
<212> PRT
<213> Artificial Sequence

<220> 
<223> AX213 Antibody Fd Chain

<400> 5
Gln Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
 1               5                  10                  15      
Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Arg Tyr
            20                  25                  30          
Gly Ile Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile
        35                  40                  45              
Gly Arg Ile Asp Pro Gly Asn Gly Gly Thr Arg Tyr Asn Glu Lys Phe
    50                  55                  60                  
Lys Gly Lys Ala Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95      
Ala Arg Ala Asn Asp Gly Tyr Ser Phe Asp Tyr Trp Gly Gln Gly Thr
            100                 105                 110         
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
        115                 120                 125             
Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly
    130                 135                 140                 
Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
145                 150                 155                 160 
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln
                165                 170                 175     
Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
            180                 185                 190         
Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser
        195                 200                 205             
Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr
    210                 215                 220                 
His Thr
225     


<210> 6
<211> 678
<212> DNA
<213> Artificial Sequence

<220> 
<223> AX213 Antibody Fd Chain

<400> 6
caggtgcaat tgctggaatc tggtggtggt ctggtgcagc caggtggttc tctgcgtctg 60
tcttgcaagg ctagcggtta caccttctct cgctacggta tcaactgggt gcgtcaggca 120
ccaggtaagg gtctggaatg gatcggtcgg atcgacccag gtaacggtgg tactaggtac 180
aacgaaaagt tcaagggtaa ggccaccatc tctagagaca actctaagaa caccctgtac 240
ttgcagatga actctctgcg tgccgaggac actgcagtgt actactgcgc ccgtgcaaat 300
gacggttact ccttcgacta ctggggtcag ggtacgctgg tgactgtctc gagcgcaagc 360
accaaaggcc catcggtatt ccccctggca ccctcctcca agagcacctc tgggggcaca 420
gcggccctgg gctgcctggt caaggactac ttccccgagc cggtgacggt gtcgtggaac 480
tcaggcgctc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc 540
tactccctca gcagcgtggt gactgtgccc tccagcagct tgggcaccca gacctacatc 600
tgcaacgtga atcacaagcc cagcaacact aaggtggaca agaaagttga gcccaaatct 660
tgtgacaaaa ctcacaca                                               678

<210> 7
<211> 118
<212> PRT
<213> Artificial Sequence

<220> 
<223> AX213 Antibody Variable Heavy Region

<400> 7
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
 1               5                  10                  15      
Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Arg Tyr
            20                  25                  30          
Gly Ile Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile
        35                  40                  45              
Gly Arg Ile Asp Pro Gly Asn Gly Gly Thr Arg Tyr Asn Glu Lys Phe
    50                  55                  60                  
Lys Gly Lys Ala Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95      
Ala Arg Ala Asn Asp Gly Tyr Ser Phe Asp Tyr Trp Gly Gln Gly Thr
            100                 105                 110         
Leu Val Thr Val Ser Ser
        115             


<210> 8
<211> 354
<212> DNA
<213> Artificial Sequence

<220> 
<223> AX213 Antibody Variable Heavy Region

<400> 8
caggtgcaat tgctggaatc tggtggtggt ctggtgcagc caggtggttc tctgcgtctg 60
tcttgcaagg ctagcggtta caccttctct cgctacggta tcaactgggt gcgtcaggca 120
ccaggtaagg gtctggaatg gatcggtcgg atcgacccag gtaacggtgg tactaggtac 180
aacgaaaagt tcaagggtaa ggccaccatc tctagagaca actctaagaa caccctgtac 240
ttgcagatga actctctgcg tgccgaggac actgcagtgt actactgcgc ccgtgcaaat 300
gacggttact ccttcgacta ctggggtcag ggtacgctgg tgactgtctc gagc       354

<210> 9
<211> 444
<212> PRT
<213> Artificial Sequence

<220> 
<223> AX213 Antibody Heavy Chain

<400> 9
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
 1               5                  10                  15      
Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Arg Tyr
            20                  25                  30          
Gly Ile Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile
        35                  40                  45              
Gly Arg Ile Asp Pro Gly Asn Gly Gly Thr Arg Tyr Asn Glu Lys Phe
    50                  55                  60                  
Lys Gly Lys Ala Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95      
Ala Arg Ala Asn Asp Gly Tyr Ser Phe Asp Tyr Trp Gly Gln Gly Thr
            100                 105                 110         
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
        115                 120                 125             
Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly
    130                 135                 140                 
Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
145                 150                 155                 160 
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln
                165                 170                 175     
Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
            180                 185                 190         
Asn Phe Gly Thr Gln Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser
        195                 200                 205             
Asn Thr Lys Val Asp Lys Thr Val Glu Arg Lys Cys Cys Val Glu Cys
    210                 215                 220                 
Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe
225                 230                 235                 240 
Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val
                245                 250                 255     
Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Gln Phe
            260                 265                 270         
Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro
        275                 280                 285             
Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val Ser Val Leu Thr
    290                 295                 300                 
Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val
305                 310                 315                 320 
Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr
                325                 330                 335     
Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg
            340                 345                 350         
Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly
        355                 360                 365             
Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro
    370                 375                 380                 
Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp Ser Asp Gly Ser
385                 390                 395                 400 
Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln
                405                 410                 415     
Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His
            420                 425                 430         
Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 


<210> 10
<211> 1332
<212> DNA
<213> Artificial Sequence

<220> 
<223> AX213 Antibody Heavy Chain

<400> 10
gaggtccaac ttttggagtc tggaggagga ctggtccaac ctggaggctc cctgagactg 60
tcctgtaagg catctggcta caccttcagc agatatggca tcaactgggt gagacaggct 120
cctggcaagg gattggagtg gattggcagg attgaccctg gcaatggagg caccagatac 180
aatgagaagt tcaagggcaa ggctaccatc agcagggaca acagcaagaa caccctctac 240
ctccaaatga actccctgag ggctgaggac acagcagtct actactgtgc cagggctaat 300
gatggctact cctttgacta ctggggacaa ggcaccctgg tgacagtgtc ctctgctagc 360
accaagggcc catcggtctt ccccctggcg ccctgctcca ggagcacctc cgagagcaca 420
gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac 480
tcaggcgctc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc 540
tactccctca gcagcgtggt gaccgtgccc tccagcaact tcggcaccca gacctacacc 600
tgcaacgtag atcacaagcc cagcaacacc aaggtggaca agacagttga gcgcaaatgt 660
tgtgtcgagt gcccaccgtg cccagcacca cctgtggcag gaccgtcagt cttcctcttc 720
cccccaaaac ccaaggacac cctcatgatc tcccggaccc ctgaggtcac gtgcgtggtg 780
gtggacgtga gccacgaaga ccccgaggtc cagttcaact ggtacgtgga cggcgtggag 840
gtgcataatg ccaagacaaa gccacgggag gagcagttca acagcacgtt ccgtgtggtc 900
agcgtcctca ccgtcgtgca ccaggactgg ctgaacggca aggagtacaa gtgcaaggtc 960
tccaacaaag gcctcccagc ccccatcgag aaaaccatct ccaaaaccaa agggcagccc 1020
cgagaaccac aggtgtacac cctgccccca tcccgggagg agatgaccaa gaaccaggtc 1080
agcctgacct gcctggtcaa aggcttctac cccagcgaca tcgccgtgga gtgggagagc 1140
aatgggcagc cggagaacaa ctacaagacc acacctccca tgctggactc cgacggctcc 1200
ttcttcctct acagcaagct caccgtggac aagagcaggt ggcagcaggg gaacgtcttc 1260
tcatgctccg tgatgcatga ggctctgcac aaccactaca cacagaagag cctctccctg 1320
tctccgggta aa                                                     1332

<210> 11
<211> 215
<212> PRT
<213> Artificial Sequence

<220> 
<223> AX213 Antibody Light Chain

<400> 11
Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly
 1               5                  10                  15      
Glu Arg Ala Thr Ile Thr Cys Arg Ala Ser Gln Tyr Val Gly Ser Tyr
            20                  25                  30          
Leu Asn Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile
        35                  40                  45              
Tyr Asp Ala Ser Asn Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro
65                  70                  75                  80  
Glu Asp Phe Ala Val Tyr Tyr Cys Gln Val Trp Asp Ser Ser Pro Pro
                85                  90                  95      
Val Val Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala
            100                 105                 110         
Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser
        115                 120                 125             
Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu
    130                 135                 140                 
Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser
145                 150                 155                 160 
Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu
                165                 170                 175     
Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val
            180                 185                 190         
Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys
        195                 200                 205             
Ser Phe Asn Arg Gly Glu Cys
    210                 215 


<210> 12
<211> 645
<212> DNA
<213> Artificial Sequence

<220> 
<223> AX213 Antibody Light Chain

<400> 12
gagattgtgc tgacccagag ccctgccacc ctgtccctga gccctggaga gagggctacc 60
atcacttgta gggcaagcca atatgtgggc tcctacctga actggtatca acagaagcct 120
ggacaagccc caagactgct gatttatgat gccagcaaca gggctacagg catccctgcc 180
aggttctctg gctctggctc tggcacagac ttcaccctga ccatctcctc cttggaacct 240
gaggactttg ctgtctacta ctgtcaggtg tgggactcca gccctcctgt ggtgtttgga 300
ggaggcacca aggtggagat taagcgtacg gtggctgcac catctgtctt catcttcccg 360
ccatctgatg agcagttgaa atctggaact gcctctgttg tgtgcctgct gaataacttc 420
tatcccagag aggccaaagt acagtggaag gtggataacg ccctccaatc gggtaactcc 480
caggagagtg tcacagagca ggacagcaag gacagcacct acagcctcag cagcaccctg 540
acgctgagca aagcagacta cgagaaacac aaagtctacg cctgcgaagt cacccatcag 600
ggcctgagct cgcccgtcac aaagagcttc aacaggggag agtgt                 645

<210> 13
<211> 216
<212> PRT
<213> Artificial Sequence

<220> 
<223> AX1 Antibody Light Chain

<400> 13
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
 1               5                  10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Ser Arg Tyr
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
        35                  40                  45              
Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Ala Ala Tyr Asp Tyr Ser Leu Gly
                85                  90                  95      
Gly Tyr Val Phe Gly Asp Gly Thr Lys Val Glu Ile Lys Arg Thr Val
            100                 105                 110         
Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys
        115                 120                 125             
Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg
    130                 135                 140                 
Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn
145                 150                 155                 160 
Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser
                165                 170                 175     
Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys
            180                 185                 190         
Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr
        195                 200                 205             
Lys Ser Phe Asn Arg Gly Glu Cys
    210                 215     


<210> 14
<211> 648
<212> DNA
<213> Artificial Sequence

<220> 
<223> AX1 Antibody Light Chain

<400> 14
gacatccaga tgacccagtc tccatcttct ctgtctgcct ctgtgggcga ccgggtgacc 60
atcacctgcc gtgcctctca ggatatctct aggtatctgg cctggtatca gcagaagcca 120
ggtaaggcgc caaagctgct gatctacgcc gcctcttctt tgcagtctgg tgtgccatct 180
cgtttctctg gttctggttc tggcaccgac ttcaccctga ccatctcttc tttgcagcca 240
gaagacttcg ccacctacta ctgcgcggct tacgactatt ctttgggcgg ttacgtgttc 300
ggtgatggta ccaaagtgga gatcaaacgt acggtggctg caccatctgt cttcatcttc 360
ccgccatctg atgagcagtt gaaatctgga actgcctctg ttgtgtgcct gctgaataac 420
ttctatccca gagaggccaa agtacagtgg aaggtggata acgccctcca atcgggtaac 480
tcccaggaga gtgtcacaga gcaggacagc aaggacagca cctacagcct cagcagcacc 540
ctgacgctga gcaaagcaga ctacgagaaa cacaaagtct acgcctgcga agtcacccat 600
cagggcctga gctcgcccgt cacaaagagc ttcaacaggg gagagtgt              648

<210> 15
<211> 109
<212> PRT
<213> Artificial Sequence

<220> 
<223> AX1 Antibody Variable Light Region

<400> 15
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
 1               5                  10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Ser Arg Tyr
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
        35                  40                  45              
Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Ala Ala Tyr Asp Tyr Ser Leu Gly
                85                  90                  95      
Gly Tyr Val Phe Gly Asp Gly Thr Lys Val Glu Ile Lys
            100                 105                 


<210> 16
<211> 327
<212> DNA
<213> Artificial Sequence

<220> 
<223> AX1 Antibody Variable Light Region

<400> 16
gacatccaga tgacccagtc tccatcttct ctgtctgcct ctgtgggcga ccgggtgacc 60
atcacctgcc gtgcctctca ggatatctct aggtatctgg cctggtatca gcagaagcca 120
ggtaaggcgc caaagctgct gatctacgcc gcctcttctt tgcagtctgg tgtgccatct 180
cgtttctctg gttctggttc tggcaccgac ttcaccctga ccatctcttc tttgcagcca 240
gaagacttcg ccacctacta ctgcgcggct tacgactatt ctttgggcgg ttacgtgttc 300
ggtgatggta ccaaagtgga gatcaaa                                     327

<210> 17
<211> 227
<212> PRT
<213> Artificial Sequence

<220> 
<223> AX1 Antibody Fd Chain

<400> 17
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
 1               5                  10                  15      
Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Phe Thr Phe Thr Ser Tyr
            20                  25                  30          
Tyr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile
        35                  40                  45              
Gly Arg Ile Asn Pro Asp Ser Gly Ser Thr Lys Tyr Asn Glu Lys Phe
    50                  55                  60                  
Lys Gly Arg Ala Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95      
Ala Arg Gly Gly Arg Leu Ser Trp Asp Phe Asp Val Trp Gly Gln Gly
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe
        115                 120                 125             
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu
    130                 135                 140                 
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp
145                 150                 155                 160 
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu
                165                 170                 175     
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser
            180                 185                 190         
Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro
        195                 200                 205             
Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys
    210                 215                 220                 
Thr His Thr
225         


<210> 18
<211> 681
<212> DNA
<213> Artificial Sequence

<220> 
<223> AX1 Antibody Fd Chain

<400> 18
gaagtgcagc tgctggaatc tggtggtggt ctggtgcagc caggtggttc tctgcgtctg 60
tcttgcaagg cctctggttt caccttcact tcttactaca tgcactgggt gcgtcaggca 120
ccaggtaagg gtctggaatg gatcggtcgg atcaacccag attctggtag tactaagtac 180
aacgagaagt tcaagggtcg tgccaccatc tctagagaca actctaagaa caccctgtac 240
ttgcagatga actctctgcg tgccgaggac actgcagtgt actactgcgc ccgtggtggt 300
cgtttatcct gggacttcga cgtctggggt cagggtacgc tggtgactgt ctcgagcgca 360
agcaccaaag gcccatcggt attccccctg gcaccctcct ccaagagcac ctctgggggc 420
acagcggccc tgggctgcct ggtcaaggac tacttccccg agccggtgac ggtgtcgtgg 480
aactcaggcg ctctgaccag cggcgtgcac accttcccgg ctgtcctaca gtcctcagga 540
ctctactccc tcagcagcgt ggtgactgtg ccctccagca gcttgggcac ccagacctac 600
atctgcaacg tgaatcacaa gcccagcaac actaaggtgg acaagaaagt tgagcccaaa 660
tcttgtgaca aaactcacac a                                           681

<210> 19
<211> 119
<212> PRT
<213> Artificial Sequence

<220> 
<223> AX1 Antibody Variable Heavy Region

<400> 19
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
 1               5                  10                  15      
Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Phe Thr Phe Thr Ser Tyr
            20                  25                  30          
Tyr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile
        35                  40                  45              
Gly Arg Ile Asn Pro Asp Ser Gly Ser Thr Lys Tyr Asn Glu Lys Phe
    50                  55                  60                  
Lys Gly Arg Ala Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95      
Ala Arg Gly Gly Arg Leu Ser Trp Asp Phe Asp Val Trp Gly Gln Gly
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser
        115                 


<210> 20
<211> 357
<212> DNA
<213> Artificial Sequence

<220> 
<223> AX1 Antibody Variable Heavy Region

<400> 20
gaagtgcagc tgctggaatc tggtggtggt ctggtgcagc caggtggttc tctgcgtctg 60
tcttgcaagg cctctggttt caccttcact tcttactaca tgcactgggt gcgtcaggca 120
ccaggtaagg gtctggaatg gatcggtcgg atcaacccag attctggtag tactaagtac 180
aacgagaagt tcaagggtcg tgccaccatc tctagagaca actctaagaa caccctgtac 240
ttgcagatga actctctgcg tgccgaggac actgcagtgt actactgcgc ccgtggtggt 300
cgtttatcct gggacttcga cgtctggggt cagggtacgc tggtgactgt ctcgagc    357

<210> 21
<211> 445
<212> PRT
<213> Artificial Sequence

<220> 
<223> AX1 Antibody Heavy Chain

<400> 21
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
 1               5                  10                  15      
Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Phe Thr Phe Thr Ser Tyr
            20                  25                  30          
Tyr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile
        35                  40                  45              
Gly Arg Ile Asn Pro Asp Ser Gly Ser Thr Lys Tyr Asn Glu Lys Phe
    50                  55                  60                  
Lys Gly Arg Ala Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95      
Ala Arg Gly Gly Arg Leu Ser Trp Asp Phe Asp Val Trp Gly Gln Gly
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe
        115                 120                 125             
Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu
    130                 135                 140                 
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp
145                 150                 155                 160 
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu
                165                 170                 175     
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser
            180                 185                 190         
Ser Asn Phe Gly Thr Gln Thr Tyr Thr Cys Asn Val Asp His Lys Pro
        195                 200                 205             
Ser Asn Thr Lys Val Asp Lys Thr Val Glu Arg Lys Cys Cys Val Glu
    210                 215                 220                 
Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu
225                 230                 235                 240 
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu
                245                 250                 255     
Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Gln
            260                 265                 270         
Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys
        275                 280                 285             
Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val Ser Val Leu
    290                 295                 300                 
Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys
305                 310                 315                 320 
Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys
                325                 330                 335     
Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser
            340                 345                 350         
Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys
        355                 360                 365             
Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln
    370                 375                 380                 
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp Ser Asp Gly
385                 390                 395                 400 
Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln
                405                 410                 415     
Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn
            420                 425                 430         
His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 


<210> 22
<211> 1335
<212> DNA
<213> Artificial Sequence

<220> 
<223> AX1 Antibody Heavy Chain

<400> 22
gaggtccaac ttttggagtc tggaggagga ctggtccaac ctggaggctc cctgagactg 60
tcctgtaagg catctggctt caccttcacc tcctactata tgcactgggt gagacaggct 120
cctggcaagg gattggagtg gattggcagg ataaaccctg actctggcag caccaaatac 180
aatgagaagt tcaagggcag ggctaccatc agcagggaca acagcaagaa caccctctac 240
ctccaaatga actccctgag ggctgaggac acagcagtct actactgtgc caggggaggc 300
agactgtcct gggactttga tgtgtgggga caaggcaccc tggtgacagt gtcctctgct 360
agcaccaagg gcccatcggt cttccccctg gcgccctgct ccaggagcac ctccgagagc 420
acagcggccc tgggctgcct ggtcaaggac tacttccccg aaccggtgac ggtgtcgtgg 480
aactcaggcg ctctgaccag cggcgtgcac accttcccgg ctgtcctaca gtcctcagga 540
ctctactccc tcagcagcgt ggtgaccgtg ccctccagca acttcggcac ccagacctac 600
acctgcaacg tagatcacaa gcccagcaac accaaggtgg acaagacagt tgagcgcaaa 660
tgttgtgtcg agtgcccacc gtgcccagca ccacctgtgg caggaccgtc agtcttcctc 720
ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt cacgtgcgtg 780
gtggtggacg tgagccacga agaccccgag gtccagttca actggtacgt ggacggcgtg 840
gaggtgcata atgccaagac aaagccacgg gaggagcagt tcaacagcac gttccgtgtg 900
gtcagcgtcc tcaccgtcgt gcaccaggac tggctgaacg gcaaggagta caagtgcaag 960
gtctccaaca aaggcctccc agcccccatc gagaaaacca tctccaaaac caaagggcag 1020
ccccgagaac cacaggtgta caccctgccc ccatcccggg aggagatgac caagaaccag 1080
gtcagcctga cctgcctggt caaaggcttc taccccagcg acatcgccgt ggagtgggag 1140
agcaatgggc agccggagaa caactacaag accacacctc ccatgctgga ctccgacggc 1200
tccttcttcc tctacagcaa gctcaccgtg gacaagagca ggtggcagca ggggaacgtc 1260
ttctcatgct ccgtgatgca tgaggctctg cacaaccact acacacagaa gagcctctcc 1320
ctgtctccgg gtaaa                                                  1335

<210> 23
<211> 216
<212> PRT
<213> Artificial Sequence

<220> 
<223> AX1 Antibody Light Chain

<400> 23
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
 1               5                  10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Ser Arg Tyr
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
        35                  40                  45              
Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Ala Ala Tyr Asp Tyr Ser Leu Gly
                85                  90                  95      
Gly Tyr Val Phe Gly Asp Gly Thr Lys Val Glu Ile Lys Arg Thr Val
            100                 105                 110         
Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys
        115                 120                 125             
Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg
    130                 135                 140                 
Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn
145                 150                 155                 160 
Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser
                165                 170                 175     
Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys
            180                 185                 190         
Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr
        195                 200                 205             
Lys Ser Phe Asn Arg Gly Glu Cys
    210                 215     


<210> 24
<211> 648
<212> DNA
<213> Artificial Sequence

<220> 
<223> AX1 Antibody Light Chain

<400> 24
gacatccaga tgacccagag cccatcctcc ctgtctgcct ctgtgggaga cagggtgacc 60
atcacttgta gggcaagcca ggacatcagc agatacctgg cttggtatca acagaagcct 120
ggcaaggctc caaaactgct gatttatgct gcctcctccc tccaatctgg agtgccaagc 180
aggttctctg gctctggctc tggcacagac ttcaccctga ccatctcctc cctccaacct 240
gaggactttg ccacctacta ctgtgctgcc tatgactact ccctgggagg ctatgtgttt 300
ggagatggca ccaaggtgga gattaagcgt acggtggctg caccatctgt cttcatcttc 360
ccgccatctg atgagcagtt gaaatctgga actgcctctg ttgtgtgcct gctgaataac 420
ttctatccca gagaggccaa agtacagtgg aaggtggata acgccctcca atcgggtaac 480
tcccaggaga gtgtcacaga gcaggacagc aaggacagca cctacagcct cagcagcacc 540
ctgacgctga gcaaagcaga ctacgagaaa cacaaagtct acgcctgcga agtcacccat 600
cagggcctga gctcgcccgt cacaaagagc ttcaacaggg gagagtgt              648
