                          SEQUENZPROTOKOLL

<110>  AB ENZYMES GMBH
 
<120>  KLONIERUNG, EXPRESSION UND VERWENDUNG SAURER PHOSPHOLIPASEN

<130>  20581WO

<150>  DE 10 2009 051 013.3
<151>  2009-10-28

<160>  5     

<170>  PatentIn version 3.3

<210>  1
<211>  1629
<212>  DNA
<213>  Aspergillus fumigatus


<220>
<221>  CDS
<222>  (55)..(142)

<220>
<221>  CDS
<222>  (197)..(362)

<220>
<221>  CDS
<222>  (404)..(749)

<220>
<221>  CDS
<222>  (807)..(1103)

<400>  1
agagtctgcc tatattctct ctgaaagggt tgtcttgagt atagcttcgg catc atg       57
                                                            Met         
                                                            1           

gtc cag ttc aag tct gtc cgt acg ctg gct gtc gcg gcg ttt gct gcg      105
Val Gln Phe Lys Ser Val Arg Thr Leu Ala Val Ala Ala Phe Ala Ala         
            5                   10                  15                  

ctg ggt gct gcg gcg cca gca ggg ttg gct gag cga g gtatgtccga         152
Leu Gly Ala Ala Ala Pro Ala Gly Leu Ala Glu Arg                         
        20                  25                                          

cgcttcctta agattggctc tgggtggtgc taactactaa gtag at  gtg tcc gcg     207
                                                 Asp Val Ser Ala        
                                                                        

tcg gtg ctg caa aag ttg tcg ttg ttt gcg caa tac tct gct gcc gct      255
Ser Val Leu Gln Lys Leu Ser Leu Phe Ala Gln Tyr Ser Ala Ala Ala         
    35                  40                  45                          

tat tgt acc aac aac atc aat tcc acg ggc acc aag ctg acg tgc tct      303
Tyr Cys Thr Asn Asn Ile Asn Ser Thr Gly Thr Lys Leu Thr Cys Ser         
50                  55                  60                  65          

gct gga aac tgt cct ctg gtc gag gca gcc aac acc aag acc ctt gcg      351
Ala Gly Asn Cys Pro Leu Val Glu Ala Ala Asn Thr Lys Thr Leu Ala         
                70                  75                  80              

gag ttc tac ga  gtaggtcgat cccatgcatg agtagctcgc atatctaaca g a      404
Glu Phe Tyr Glu                                                         
                                                                        

gct ggt agt tcc gaa tcg ttt gga gac acg gca ggc ttc ttg gtt gca      452
Ala Gly Ser Ser Glu Ser Phe Gly Asp Thr Ala Gly Phe Leu Val Ala         
                90                  95                  100             

gac acc aca aac aag cta ctc gtg gtc tct ttc aga gga agc cgc acg      500
Asp Thr Thr Asn Lys Leu Leu Val Val Ser Phe Arg Gly Ser Arg Thr         
            105                 110                 115                 

ata gac aac tgg att gcg aat ctg gac ttt gtt ctg gac agt gtc agt      548
Ile Asp Asn Trp Ile Ala Asn Leu Asp Phe Val Leu Asp Ser Val Ser         
        120                 125                 130                     

gat att tgc agc gga tgc gcc gca cac ggg ggc ttc tgg aag tcc tgg      596
Asp Ile Cys Ser Gly Cys Ala Ala His Gly Gly Phe Trp Lys Ser Trp         
    135                 140                 145                         

gaa gtt gtt gcc aat tcg ctg acg acc gag ctc aac tct gcg gtt aac      644
Glu Val Val Ala Asn Ser Leu Thr Thr Glu Leu Asn Ser Ala Val Asn         
150                 155                 160                 165         

act tac cct ggc tat acc att gtc ttc act gga cat agc ctc ggc gct      692
Thr Tyr Pro Gly Tyr Thr Ile Val Phe Thr Gly His Ser Leu Gly Ala         
                170                 175                 180             

gct ctt gca aca ctg ggg gct act acg ctg cgg aaa gca ggg att ccc      740
Ala Leu Ala Thr Leu Gly Ala Thr Thr Leu Arg Lys Ala Gly Ile Pro         
            185                 190                 195                 

att cag ctg gtaagtcatc ccttgtcaac ttatgcaagg gcgcaatggg              789
Ile Gln Leu                                                             
        200                                                             

actaattgat tgtgaag tat aat tac gga tcc ccc cgt gtt gga aac acg       839
                   Tyr Asn Tyr Gly Ser Pro Arg Val Gly Asn Thr          
                                   205                 210              

gcc ttg gca aca tac atc acc gca cag ggt ccc aat tac cgt gtc aca      887
Ala Leu Ala Thr Tyr Ile Thr Ala Gln Gly Pro Asn Tyr Arg Val Thr         
            215                 220                 225                 

cac aca aac gat att gtg ccc aga ctc ccg ccc caa gct ttt ggc ttc      935
His Thr Asn Asp Ile Val Pro Arg Leu Pro Pro Gln Ala Phe Gly Phe         
        230                 235                 240                     

agc cac ctt agc ccg gag tac tgg atc acg agc ggc gac aac gtg cct      983
Ser His Leu Ser Pro Glu Tyr Trp Ile Thr Ser Gly Asp Asn Val Pro         
    245                 250                 255                         

gtc acg acg tct gat atc acg gtc atc cag gga atc gac tca gac gct     1031
Val Thr Thr Ser Asp Ile Thr Val Ile Gln Gly Ile Asp Ser Asp Ala         
260                 265                 270                 275         

gga aat tcg gga gag gat atc acc agc atc gag gcc cat aat tgg tat     1079
Gly Asn Ser Gly Glu Asp Ile Thr Ser Ile Glu Ala His Asn Trp Tyr         
                280                 285                 290             

ctc ggc gat att gat gct tgt caa tgagactata agcggagtat ataacagctt    1133
Leu Gly Asp Ile Asp Ala Cys Gln                                         
            295                                                         

tggatagtat aaaagggcca gtacacttgg gctaacgcat gaggaatgac attgatgacc   1193

tatcttgcca atgcaatcag ttttataagg agagtcctca tgattgatta tgtcaattgg   1253

tatggagtag aaataaactg tacagatctc tggatccgcc gagtggacat tcattatgag   1313

gttctgggga agtttgtttg gtttggactt tgacacctgg agtttatccc catctccatc   1373

aactcgtctg attgtggctc gacgagcgca ttcttactga atgctcatct gtttgaatag   1433

aatatgatta acgagcagta actcccattc ctttcgaacg cctttgcgca attgaatcca   1493

tccttccaac ccgtgcaact tcaaccagcc gcccgggcga ctctgcgcat tctcaacatc   1553

tctcgacccg ccgcgatggt cgctgctcca tgctgctgat actcttctgt tatcagtaat   1613

cacggaaatt gtcata                                                   1629


<210>  2
<211>  299
<212>  PRT
<213>  Aspergillus fumigatus

<400>  2

Met Val Gln Phe Lys Ser Val Arg Thr Leu Ala Val Ala Ala Phe Ala 
1               5                   10                  15      


Ala Leu Gly Ala Ala Ala Pro Ala Gly Leu Ala Glu Arg Asp Val Ser 
            20                  25                  30          


Ala Ser Val Leu Gln Lys Leu Ser Leu Phe Ala Gln Tyr Ser Ala Ala 
        35                  40                  45              


Ala Tyr Cys Thr Asn Asn Ile Asn Ser Thr Gly Thr Lys Leu Thr Cys 
    50                  55                  60                  


Ser Ala Gly Asn Cys Pro Leu Val Glu Ala Ala Asn Thr Lys Thr Leu 
65                  70                  75                  80  


Ala Glu Phe Tyr Glu Ala Gly Ser Ser Glu Ser Phe Gly Asp Thr Ala 
                85                  90                  95      


Gly Phe Leu Val Ala Asp Thr Thr Asn Lys Leu Leu Val Val Ser Phe 
            100                 105                 110         


Arg Gly Ser Arg Thr Ile Asp Asn Trp Ile Ala Asn Leu Asp Phe Val 
        115                 120                 125             


Leu Asp Ser Val Ser Asp Ile Cys Ser Gly Cys Ala Ala His Gly Gly 
    130                 135                 140                 


Phe Trp Lys Ser Trp Glu Val Val Ala Asn Ser Leu Thr Thr Glu Leu 
145                 150                 155                 160 


Asn Ser Ala Val Asn Thr Tyr Pro Gly Tyr Thr Ile Val Phe Thr Gly 
                165                 170                 175     


His Ser Leu Gly Ala Ala Leu Ala Thr Leu Gly Ala Thr Thr Leu Arg 
            180                 185                 190         


Lys Ala Gly Ile Pro Ile Gln Leu Tyr Asn Tyr Gly Ser Pro Arg Val 
        195                 200                 205             


Gly Asn Thr Ala Leu Ala Thr Tyr Ile Thr Ala Gln Gly Pro Asn Tyr 
    210                 215                 220                 


Arg Val Thr His Thr Asn Asp Ile Val Pro Arg Leu Pro Pro Gln Ala 
225                 230                 235                 240 


Phe Gly Phe Ser His Leu Ser Pro Glu Tyr Trp Ile Thr Ser Gly Asp 
                245                 250                 255     


Asn Val Pro Val Thr Thr Ser Asp Ile Thr Val Ile Gln Gly Ile Asp 
            260                 265                 270         


Ser Asp Ala Gly Asn Ser Gly Glu Asp Ile Thr Ser Ile Glu Ala His 
        275                 280                 285             


Asn Trp Tyr Leu Gly Asp Ile Asp Ala Cys Gln 
    290                 295                 


<210>  3
<211>  16
<212>  PRT
<213>  Aspergillus fumigatus

<400>  3

Asp Val Ser Ala Ser Val Leu Gln Lys Leu Ser Leu Phe Ala Gln Tyr 
1               5                   10                  15      


<210>  4
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  4
agagtctgcc tatattctct ctgaaagg                                        28


<210>  5
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  5
tatgacaatt tccgtgatta ctg                                             23
