                         SEQUENCE LISTING

<110>  Georg-August-Universitaet Goettingen Stiftung Oeffentlichen
        Rechts
       Feussner, Ivo
       Heilmann, Mareike
 
<120>  METHODS AND MEANS TO ALTER LIPID BIOSYNTHESIS BY TARGETING 
       MULTIPLE ENZYMES TO SUBORGANELLE DOMAINS

<130>  B09165A - CA

<150>  EP09013248.1
<151>  2009-10-20

<160>  16    

<170>  PatentIn version 3.3

<210>  1
<211>  1548
<212>  DNA
<213>  Artificial

<220>
<223>  mouse fatty acyl CoA reductase


<220>
<221>  CDS
<222>  (1)..(1548)

<400>  1
atg gtt tcc atc cca gag tac tat gag gga aag aac atc ttg cta act       48
Met Val Ser Ile Pro Glu Tyr Tyr Glu Gly Lys Asn Ile Leu Leu Thr         
1               5                   10                  15              

ggt gca act gga ttt ctt ggg aag gtt ttg ctt gag aag ctg cta aga       96
Gly Ala Thr Gly Phe Leu Gly Lys Val Leu Leu Glu Lys Leu Leu Arg         
            20                  25                  30                  

tct tgc cct aga gtc aac tcc gtt tac gtt cta gtc aga caa aag gct      144
Ser Cys Pro Arg Val Asn Ser Val Tyr Val Leu Val Arg Gln Lys Ala         
        35                  40                  45                      

gga caa aca cct caa gaa aga gtt gag gag atc ttg tca agc aag ctc      192
Gly Gln Thr Pro Gln Glu Arg Val Glu Glu Ile Leu Ser Ser Lys Leu         
    50                  55                  60                          

ttt gat aga ctg cgt gat gag aat cca gat ttc cga gag aag atc atc      240
Phe Asp Arg Leu Arg Asp Glu Asn Pro Asp Phe Arg Glu Lys Ile Ile         
65                  70                  75                  80          

gcc atc aac tct gaa ttg acc caa cct aag tta gcg ctt tct gaa gag      288
Ala Ile Asn Ser Glu Leu Thr Gln Pro Lys Leu Ala Leu Ser Glu Glu         
                85                  90                  95              

gat aag gag atc atc atc gac tca acg aac gtt atc ttc cat tgc gct      336
Asp Lys Glu Ile Ile Ile Asp Ser Thr Asn Val Ile Phe His Cys Ala         
            100                 105                 110                 

gca aca gtt cgt ttc aat gag aac cta cgg gat gct gtt caa ttg aac      384
Ala Thr Val Arg Phe Asn Glu Asn Leu Arg Asp Ala Val Gln Leu Asn         
        115                 120                 125                     

gtc att gct acg aga caa ctg atc tta ctc gct cag cag atg aag aac      432
Val Ile Ala Thr Arg Gln Leu Ile Leu Leu Ala Gln Gln Met Lys Asn         
    130                 135                 140                         

ctc gaa gtc ttc atg cac gtt agt act gct tac gca tac tgt aac cgc      480
Leu Glu Val Phe Met His Val Ser Thr Ala Tyr Ala Tyr Cys Asn Arg         
145                 150                 155                 160         

aag cac atc gat gaa gtt gtt tac cca cct cca gtt gat cct aag aag      528
Lys His Ile Asp Glu Val Val Tyr Pro Pro Pro Val Asp Pro Lys Lys         
                165                 170                 175             

ttg atc gac tct ctt gag tgg atg gat gat gga ctt gtg aac gac ata      576
Leu Ile Asp Ser Leu Glu Trp Met Asp Asp Gly Leu Val Asn Asp Ile         
            180                 185                 190                 

aca cca aag ctt atc gga gac aga cca aac act tac atc tac act aag      624
Thr Pro Lys Leu Ile Gly Asp Arg Pro Asn Thr Tyr Ile Tyr Thr Lys         
        195                 200                 205                     

gca ctg gct gag tat gtt gtt caa caa gag gga gct aag ttg aac gtt      672
Ala Leu Ala Glu Tyr Val Val Gln Gln Glu Gly Ala Lys Leu Asn Val         
    210                 215                 220                         

gca atc gtt aga ccg tct att gtt gga gct tca tgg aaa gaa cca ttc      720
Ala Ile Val Arg Pro Ser Ile Val Gly Ala Ser Trp Lys Glu Pro Phe         
225                 230                 235                 240         

cca gga tgg att gac aac ttc aat ggt cca tct gga ctt ttc att gca      768
Pro Gly Trp Ile Asp Asn Phe Asn Gly Pro Ser Gly Leu Phe Ile Ala         
                245                 250                 255             

gct ggc aag ggt atc ctc aga act atg aga gca agt aac aac gca ctt      816
Ala Gly Lys Gly Ile Leu Arg Thr Met Arg Ala Ser Asn Asn Ala Leu         
            260                 265                 270                 

gca gat ctt gtt cct gtt gac gtt gtc gtt aac acc tca tta gca gct      864
Ala Asp Leu Val Pro Val Asp Val Val Val Asn Thr Ser Leu Ala Ala         
        275                 280                 285                     

gca tgg tat tct gga gtt aac aga ccc agg aac atc atg gtg tac aac      912
Ala Trp Tyr Ser Gly Val Asn Arg Pro Arg Asn Ile Met Val Tyr Asn         
    290                 295                 300                         

tgt aca acg gga tct aca aac cct ttt cat tgg ggt gaa gtt gag tat      960
Cys Thr Thr Gly Ser Thr Asn Pro Phe His Trp Gly Glu Val Glu Tyr         
305                 310                 315                 320         

cac gtc atc tct acc ttc aag aga aac cct ctt gag caa gct ttc aga     1008
His Val Ile Ser Thr Phe Lys Arg Asn Pro Leu Glu Gln Ala Phe Arg         
                325                 330                 335             

aga cct aac gtg aac ctt acc tcc aat cat cta ctc tac cac tac tgg     1056
Arg Pro Asn Val Asn Leu Thr Ser Asn His Leu Leu Tyr His Tyr Trp         
            340                 345                 350                 

att gct gtt tct cat aag gct cct gct ttc ttg tac gac atc tac ctt     1104
Ile Ala Val Ser His Lys Ala Pro Ala Phe Leu Tyr Asp Ile Tyr Leu         
        355                 360                 365                     

cga atg act ggt aga agt cct cgg atg atg aag acc att aca cga cta     1152
Arg Met Thr Gly Arg Ser Pro Arg Met Met Lys Thr Ile Thr Arg Leu         
    370                 375                 380                         

cac aag gct atg gtc ttc ctt gag tac ttc acc tca aac tca tgg gtt     1200
His Lys Ala Met Val Phe Leu Glu Tyr Phe Thr Ser Asn Ser Trp Val         
385                 390                 395                 400         

tgg aac act gac aac gtt aac atg ctc atg aac cag ctt aac cct gag     1248
Trp Asn Thr Asp Asn Val Asn Met Leu Met Asn Gln Leu Asn Pro Glu         
                405                 410                 415             

gac aag aag acg ttc aac att gat gtt cga cag ctt cac tgg gct gag     1296
Asp Lys Lys Thr Phe Asn Ile Asp Val Arg Gln Leu His Trp Ala Glu         
            420                 425                 430                 

tac ata gag aac tac tgt atg ggg acg aag aag tac gtt ctt aac gaa     1344
Tyr Ile Glu Asn Tyr Cys Met Gly Thr Lys Lys Tyr Val Leu Asn Glu         
        435                 440                 445                     

gag atg tct gga ctt cca gca gct aga aaa cat ctg aac aag ctt cgc     1392
Glu Met Ser Gly Leu Pro Ala Ala Arg Lys His Leu Asn Lys Leu Arg         
    450                 455                 460                         

aac atc aga tac ggt ttc aac acc atc ctg gtt atc ctt atc tgg agg     1440
Asn Ile Arg Tyr Gly Phe Asn Thr Ile Leu Val Ile Leu Ile Trp Arg         
465                 470                 475                 480         

atc ttc atc gct aga agt caa atg gcg aga aac atc tgg tac ttc gtt     1488
Ile Phe Ile Ala Arg Ser Gln Met Ala Arg Asn Ile Trp Tyr Phe Val         
                485                 490                 495             

gtt tcg ctg tgc tac aag ttc ctt tct tac ttc aga gcc tct tcc aca     1536
Val Ser Leu Cys Tyr Lys Phe Leu Ser Tyr Phe Arg Ala Ser Ser Thr         
            500                 505                 510                 

atg cga tac taa                                                     1548
Met Arg Tyr                                                             
        515                                                             


<210>  2
<211>  515
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  2

Met Val Ser Ile Pro Glu Tyr Tyr Glu Gly Lys Asn Ile Leu Leu Thr 
1               5                   10                  15      


Gly Ala Thr Gly Phe Leu Gly Lys Val Leu Leu Glu Lys Leu Leu Arg 
            20                  25                  30          


Ser Cys Pro Arg Val Asn Ser Val Tyr Val Leu Val Arg Gln Lys Ala 
        35                  40                  45              


Gly Gln Thr Pro Gln Glu Arg Val Glu Glu Ile Leu Ser Ser Lys Leu 
    50                  55                  60                  


Phe Asp Arg Leu Arg Asp Glu Asn Pro Asp Phe Arg Glu Lys Ile Ile 
65                  70                  75                  80  


Ala Ile Asn Ser Glu Leu Thr Gln Pro Lys Leu Ala Leu Ser Glu Glu 
                85                  90                  95      


Asp Lys Glu Ile Ile Ile Asp Ser Thr Asn Val Ile Phe His Cys Ala 
            100                 105                 110         


Ala Thr Val Arg Phe Asn Glu Asn Leu Arg Asp Ala Val Gln Leu Asn 
        115                 120                 125             


Val Ile Ala Thr Arg Gln Leu Ile Leu Leu Ala Gln Gln Met Lys Asn 
    130                 135                 140                 


Leu Glu Val Phe Met His Val Ser Thr Ala Tyr Ala Tyr Cys Asn Arg 
145                 150                 155                 160 


Lys His Ile Asp Glu Val Val Tyr Pro Pro Pro Val Asp Pro Lys Lys 
                165                 170                 175     


Leu Ile Asp Ser Leu Glu Trp Met Asp Asp Gly Leu Val Asn Asp Ile 
            180                 185                 190         


Thr Pro Lys Leu Ile Gly Asp Arg Pro Asn Thr Tyr Ile Tyr Thr Lys 
        195                 200                 205             


Ala Leu Ala Glu Tyr Val Val Gln Gln Glu Gly Ala Lys Leu Asn Val 
    210                 215                 220                 


Ala Ile Val Arg Pro Ser Ile Val Gly Ala Ser Trp Lys Glu Pro Phe 
225                 230                 235                 240 


Pro Gly Trp Ile Asp Asn Phe Asn Gly Pro Ser Gly Leu Phe Ile Ala 
                245                 250                 255     


Ala Gly Lys Gly Ile Leu Arg Thr Met Arg Ala Ser Asn Asn Ala Leu 
            260                 265                 270         


Ala Asp Leu Val Pro Val Asp Val Val Val Asn Thr Ser Leu Ala Ala 
        275                 280                 285             


Ala Trp Tyr Ser Gly Val Asn Arg Pro Arg Asn Ile Met Val Tyr Asn 
    290                 295                 300                 


Cys Thr Thr Gly Ser Thr Asn Pro Phe His Trp Gly Glu Val Glu Tyr 
305                 310                 315                 320 


His Val Ile Ser Thr Phe Lys Arg Asn Pro Leu Glu Gln Ala Phe Arg 
                325                 330                 335     


Arg Pro Asn Val Asn Leu Thr Ser Asn His Leu Leu Tyr His Tyr Trp 
            340                 345                 350         


Ile Ala Val Ser His Lys Ala Pro Ala Phe Leu Tyr Asp Ile Tyr Leu 
        355                 360                 365             


Arg Met Thr Gly Arg Ser Pro Arg Met Met Lys Thr Ile Thr Arg Leu 
    370                 375                 380                 


His Lys Ala Met Val Phe Leu Glu Tyr Phe Thr Ser Asn Ser Trp Val 
385                 390                 395                 400 


Trp Asn Thr Asp Asn Val Asn Met Leu Met Asn Gln Leu Asn Pro Glu 
                405                 410                 415     


Asp Lys Lys Thr Phe Asn Ile Asp Val Arg Gln Leu His Trp Ala Glu 
            420                 425                 430         


Tyr Ile Glu Asn Tyr Cys Met Gly Thr Lys Lys Tyr Val Leu Asn Glu 
        435                 440                 445             


Glu Met Ser Gly Leu Pro Ala Ala Arg Lys His Leu Asn Lys Leu Arg 
    450                 455                 460                 


Asn Ile Arg Tyr Gly Phe Asn Thr Ile Leu Val Ile Leu Ile Trp Arg 
465                 470                 475                 480 


Ile Phe Ile Ala Arg Ser Gln Met Ala Arg Asn Ile Trp Tyr Phe Val 
                485                 490                 495     


Val Ser Leu Cys Tyr Lys Phe Leu Ser Tyr Phe Arg Ala Ser Ser Thr 
            500                 505                 510         


Met Arg Tyr 
        515 


<210>  3
<211>  2274
<212>  DNA
<213>  Artificial

<220>
<223>  mouse fatty acyl CoA reductase tagged with mCherry


<220>
<221>  CDS
<222>  (7)..(2268)

<400>  3
ggatcc atg gtg agc aag ggc gag gag gat aac atg gcc atc atc aag        48
       Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys          
       1               5                   10                           

gag ttc atg cgc ttc aag gtg cac atg gag ggc tcc gtg aac ggc cac       96
Glu Phe Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His         
15                  20                  25                  30          

gag ttc gag atc gag ggc gag ggc gag ggc cgc ccc tac gag ggc acc      144
Glu Phe Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr         
                35                  40                  45              

cag acc gcc aag ctg aag gtg acc aag ggt ggc ccc ctg ccc ttc gcc      192
Gln Thr Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala         
            50                  55                  60                  

tgg gac atc ctg tcc cct cag ttc atg tac ggc tcc aag gcc tac gtg      240
Trp Asp Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val         
        65                  70                  75                      

aag cac ccc gcc gac atc ccc gac tac ttg aag ctg tcc ttc ccc gag      288
Lys His Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu         
    80                  85                  90                          

ggc ttc aag tgg gag cgc gtg atg aac ttc gag gac ggc ggc gtg gtg      336
Gly Phe Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val         
95                  100                 105                 110         

acc gtg acc cag gac tcc tcc ctg cag gac ggc gag ttc atc tac aag      384
Thr Val Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys         
                115                 120                 125             

gtg aag ctg cgc ggc acc aac ttc ccc tcc gac ggc ccc gta atg cag      432
Val Lys Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln         
            130                 135                 140                 

aag aag acc atg ggc tgg gag gcc tcc tcc gag cgg atg tac ccc gag      480
Lys Lys Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu         
        145                 150                 155                     

gac ggc gcc ctg aag ggc gag atc aag cag agg ctg aag ctg aag gac      528
Asp Gly Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp         
    160                 165                 170                         

ggc ggc cac tac gac gct gag gtc aag acc acc tac aag gcc aag aag      576
Gly Gly His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys         
175                 180                 185                 190         

ccc gtg cag ctg ccc ggc gcc tac aac gtc aac atc aag ttg gac atc      624
Pro Val Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile         
                195                 200                 205             

acc tcc cac aac gag gac tac acc atc gtg gaa cag tac gaa cgc gcc      672
Thr Ser His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala         
            210                 215                 220                 

gag ggc cgc cac tcc acc ggc ggc atg gac gag ctg tac aag gaa ttc      720
Glu Gly Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys Glu Phe         
        225                 230                 235                     

atg gtt tcc atc cca gag tac tat gag gga aag aac atc ttg cta act      768
Met Val Ser Ile Pro Glu Tyr Tyr Glu Gly Lys Asn Ile Leu Leu Thr         
    240                 245                 250                         

ggt gca act gga ttt ctt ggg aag gtt ttg ctt gag aag ctg cta aga      816
Gly Ala Thr Gly Phe Leu Gly Lys Val Leu Leu Glu Lys Leu Leu Arg         
255                 260                 265                 270         

tct tgc cct aga gtc aac tcc gtt tac gtt cta gtc aga caa aag gct      864
Ser Cys Pro Arg Val Asn Ser Val Tyr Val Leu Val Arg Gln Lys Ala         
                275                 280                 285             

gga caa aca cct caa gaa aga gtt gag gag atc ttg tca agc aag ctc      912
Gly Gln Thr Pro Gln Glu Arg Val Glu Glu Ile Leu Ser Ser Lys Leu         
            290                 295                 300                 

ttt gat aga ctg cgt gat gag aat cca gat ttc cga gag aag atc atc      960
Phe Asp Arg Leu Arg Asp Glu Asn Pro Asp Phe Arg Glu Lys Ile Ile         
        305                 310                 315                     

gcc atc aac tct gaa ttg acc caa cct aag tta gcg ctt tct gaa gag     1008
Ala Ile Asn Ser Glu Leu Thr Gln Pro Lys Leu Ala Leu Ser Glu Glu         
    320                 325                 330                         

gat aag gag atc atc atc gac tca acg aac gtt atc ttc cat tgc gct     1056
Asp Lys Glu Ile Ile Ile Asp Ser Thr Asn Val Ile Phe His Cys Ala         
335                 340                 345                 350         

gca aca gtt cgt ttc aat gag aac cta cgg gat gct gtt caa ttg aac     1104
Ala Thr Val Arg Phe Asn Glu Asn Leu Arg Asp Ala Val Gln Leu Asn         
                355                 360                 365             

gtc att gct acg aga caa ctg atc tta ctc gct cag cag atg aag aac     1152
Val Ile Ala Thr Arg Gln Leu Ile Leu Leu Ala Gln Gln Met Lys Asn         
            370                 375                 380                 

ctc gaa gtc ttc atg cac gtt agt act gct tac gca tac tgt aac cgc     1200
Leu Glu Val Phe Met His Val Ser Thr Ala Tyr Ala Tyr Cys Asn Arg         
        385                 390                 395                     

aag cac atc gat gaa gtt gtt tac cca cct cca gtt gat cct aag aag     1248
Lys His Ile Asp Glu Val Val Tyr Pro Pro Pro Val Asp Pro Lys Lys         
    400                 405                 410                         

ttg atc gac tct ctt gag tgg atg gat gat gga ctt gtg aac gac ata     1296
Leu Ile Asp Ser Leu Glu Trp Met Asp Asp Gly Leu Val Asn Asp Ile         
415                 420                 425                 430         

aca cca aag ctt atc gga gac aga cca aac act tac atc tac act aag     1344
Thr Pro Lys Leu Ile Gly Asp Arg Pro Asn Thr Tyr Ile Tyr Thr Lys         
                435                 440                 445             

gca ctg gct gag tat gtt gtt caa caa gag gga gct aag ttg aac gtt     1392
Ala Leu Ala Glu Tyr Val Val Gln Gln Glu Gly Ala Lys Leu Asn Val         
            450                 455                 460                 

gca atc gtt aga ccg tct att gtt gga gct tca tgg aaa gaa cca ttc     1440
Ala Ile Val Arg Pro Ser Ile Val Gly Ala Ser Trp Lys Glu Pro Phe         
        465                 470                 475                     

cca gga tgg att gac aac ttc aat ggt cca tct gga ctt ttc att gca     1488
Pro Gly Trp Ile Asp Asn Phe Asn Gly Pro Ser Gly Leu Phe Ile Ala         
    480                 485                 490                         

gct ggc aag ggt atc ctc aga act atg aga gca agt aac aac gca ctt     1536
Ala Gly Lys Gly Ile Leu Arg Thr Met Arg Ala Ser Asn Asn Ala Leu         
495                 500                 505                 510         

gca gat ctt gtt cct gtt gac gtt gtc gtt aac acc tca tta gca gct     1584
Ala Asp Leu Val Pro Val Asp Val Val Val Asn Thr Ser Leu Ala Ala         
                515                 520                 525             

gca tgg tat tct gga gtt aac aga ccc agg aac atc atg gtg tac aac     1632
Ala Trp Tyr Ser Gly Val Asn Arg Pro Arg Asn Ile Met Val Tyr Asn         
            530                 535                 540                 

tgt aca acg gga tct aca aac cct ttt cat tgg ggt gaa gtt gag tat     1680
Cys Thr Thr Gly Ser Thr Asn Pro Phe His Trp Gly Glu Val Glu Tyr         
        545                 550                 555                     

cac gtc atc tct acc ttc aag aga aac cct ctt gag caa gct ttc aga     1728
His Val Ile Ser Thr Phe Lys Arg Asn Pro Leu Glu Gln Ala Phe Arg         
    560                 565                 570                         

aga cct aac gtg aac ctt acc tcc aat cat cta ctc tac cac tac tgg     1776
Arg Pro Asn Val Asn Leu Thr Ser Asn His Leu Leu Tyr His Tyr Trp         
575                 580                 585                 590         

att gct gtt tct cat aag gct cct gct ttc ttg tac gac atc tac ctt     1824
Ile Ala Val Ser His Lys Ala Pro Ala Phe Leu Tyr Asp Ile Tyr Leu         
                595                 600                 605             

cga atg act ggt aga agt cct cgg atg atg aag acc att aca cga cta     1872
Arg Met Thr Gly Arg Ser Pro Arg Met Met Lys Thr Ile Thr Arg Leu         
            610                 615                 620                 

cac aag gct atg gtc ttc ctt gag tac ttc acc tca aac tca tgg gtt     1920
His Lys Ala Met Val Phe Leu Glu Tyr Phe Thr Ser Asn Ser Trp Val         
        625                 630                 635                     

tgg aac act gac aac gtt aac atg ctc atg aac cag ctt aac cct gag     1968
Trp Asn Thr Asp Asn Val Asn Met Leu Met Asn Gln Leu Asn Pro Glu         
    640                 645                 650                         

gac aag aag acg ttc aac att gat gtt cga cag ctt cac tgg gct gag     2016
Asp Lys Lys Thr Phe Asn Ile Asp Val Arg Gln Leu His Trp Ala Glu         
655                 660                 665                 670         

tac ata gag aac tac tgt atg ggg acg aag aag tac gtt ctt aac gaa     2064
Tyr Ile Glu Asn Tyr Cys Met Gly Thr Lys Lys Tyr Val Leu Asn Glu         
                675                 680                 685             

gag atg tct gga ctt cca gca gct aga aaa cat ctg aac aag ctt cgc     2112
Glu Met Ser Gly Leu Pro Ala Ala Arg Lys His Leu Asn Lys Leu Arg         
            690                 695                 700                 

aac atc aga tac ggt ttc aac acc atc ctg gtt atc ctt atc tgg agg     2160
Asn Ile Arg Tyr Gly Phe Asn Thr Ile Leu Val Ile Leu Ile Trp Arg         
        705                 710                 715                     

atc ttc atc gct aga agt caa atg gcg aga aac atc tgg tac ttc gtt     2208
Ile Phe Ile Ala Arg Ser Gln Met Ala Arg Asn Ile Trp Tyr Phe Val         
    720                 725                 730                         

gtt tcg ctg tgc tac aag ttc ctt tct tac ttc aga gcc tct tcc aca     2256
Val Ser Leu Cys Tyr Lys Phe Leu Ser Tyr Phe Arg Ala Ser Ser Thr         
735                 740                 745                 750         

atg cga tac taa ctcgag                                              2274
Met Arg Tyr                                                             
                                                                        


<210>  4
<211>  753
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  4

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe 
1               5                   10                  15      


Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe 
            20                  25                  30          


Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
        35                  40                  45              


Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
    50                  55                  60                  


Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His 
65                  70                  75                  80  


Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
                85                  90                  95      


Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
            100                 105                 110         


Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
        115                 120                 125             


Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly 
145                 150                 155                 160 


Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly 
                165                 170                 175     


His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val 
            180                 185                 190         


Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser 
        195                 200                 205             


His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
    210                 215                 220                 


Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys Glu Phe Met Val 
225                 230                 235                 240 


Ser Ile Pro Glu Tyr Tyr Glu Gly Lys Asn Ile Leu Leu Thr Gly Ala 
                245                 250                 255     


Thr Gly Phe Leu Gly Lys Val Leu Leu Glu Lys Leu Leu Arg Ser Cys 
            260                 265                 270         


Pro Arg Val Asn Ser Val Tyr Val Leu Val Arg Gln Lys Ala Gly Gln 
        275                 280                 285             


Thr Pro Gln Glu Arg Val Glu Glu Ile Leu Ser Ser Lys Leu Phe Asp 
    290                 295                 300                 


Arg Leu Arg Asp Glu Asn Pro Asp Phe Arg Glu Lys Ile Ile Ala Ile 
305                 310                 315                 320 


Asn Ser Glu Leu Thr Gln Pro Lys Leu Ala Leu Ser Glu Glu Asp Lys 
                325                 330                 335     


Glu Ile Ile Ile Asp Ser Thr Asn Val Ile Phe His Cys Ala Ala Thr 
            340                 345                 350         


Val Arg Phe Asn Glu Asn Leu Arg Asp Ala Val Gln Leu Asn Val Ile 
        355                 360                 365             


Ala Thr Arg Gln Leu Ile Leu Leu Ala Gln Gln Met Lys Asn Leu Glu 
    370                 375                 380                 


Val Phe Met His Val Ser Thr Ala Tyr Ala Tyr Cys Asn Arg Lys His 
385                 390                 395                 400 


Ile Asp Glu Val Val Tyr Pro Pro Pro Val Asp Pro Lys Lys Leu Ile 
                405                 410                 415     


Asp Ser Leu Glu Trp Met Asp Asp Gly Leu Val Asn Asp Ile Thr Pro 
            420                 425                 430         


Lys Leu Ile Gly Asp Arg Pro Asn Thr Tyr Ile Tyr Thr Lys Ala Leu 
        435                 440                 445             


Ala Glu Tyr Val Val Gln Gln Glu Gly Ala Lys Leu Asn Val Ala Ile 
    450                 455                 460                 


Val Arg Pro Ser Ile Val Gly Ala Ser Trp Lys Glu Pro Phe Pro Gly 
465                 470                 475                 480 


Trp Ile Asp Asn Phe Asn Gly Pro Ser Gly Leu Phe Ile Ala Ala Gly 
                485                 490                 495     


Lys Gly Ile Leu Arg Thr Met Arg Ala Ser Asn Asn Ala Leu Ala Asp 
            500                 505                 510         


Leu Val Pro Val Asp Val Val Val Asn Thr Ser Leu Ala Ala Ala Trp 
        515                 520                 525             


Tyr Ser Gly Val Asn Arg Pro Arg Asn Ile Met Val Tyr Asn Cys Thr 
    530                 535                 540                 


Thr Gly Ser Thr Asn Pro Phe His Trp Gly Glu Val Glu Tyr His Val 
545                 550                 555                 560 


Ile Ser Thr Phe Lys Arg Asn Pro Leu Glu Gln Ala Phe Arg Arg Pro 
                565                 570                 575     


Asn Val Asn Leu Thr Ser Asn His Leu Leu Tyr His Tyr Trp Ile Ala 
            580                 585                 590         


Val Ser His Lys Ala Pro Ala Phe Leu Tyr Asp Ile Tyr Leu Arg Met 
        595                 600                 605             


Thr Gly Arg Ser Pro Arg Met Met Lys Thr Ile Thr Arg Leu His Lys 
    610                 615                 620                 


Ala Met Val Phe Leu Glu Tyr Phe Thr Ser Asn Ser Trp Val Trp Asn 
625                 630                 635                 640 


Thr Asp Asn Val Asn Met Leu Met Asn Gln Leu Asn Pro Glu Asp Lys 
                645                 650                 655     


Lys Thr Phe Asn Ile Asp Val Arg Gln Leu His Trp Ala Glu Tyr Ile 
            660                 665                 670         


Glu Asn Tyr Cys Met Gly Thr Lys Lys Tyr Val Leu Asn Glu Glu Met 
        675                 680                 685             


Ser Gly Leu Pro Ala Ala Arg Lys His Leu Asn Lys Leu Arg Asn Ile 
    690                 695                 700                 


Arg Tyr Gly Phe Asn Thr Ile Leu Val Ile Leu Ile Trp Arg Ile Phe 
705                 710                 715                 720 


Ile Ala Arg Ser Gln Met Ala Arg Asn Ile Trp Tyr Phe Val Val Ser 
                725                 730                 735     


Leu Cys Tyr Lys Phe Leu Ser Tyr Phe Arg Ala Ser Ser Thr Met Arg 
            740                 745                 750         


Tyr 
    


<210>  5
<211>  1404
<212>  DNA
<213>  Artificial

<220>
<223>  mouse fatty acyl CoA reductase truncated


<220>
<221>  CDS
<222>  (1)..(1404)

<400>  5
atg gtt tcc atc cca gag tac tat gag gga aag aac atc ttg cta act       48
Met Val Ser Ile Pro Glu Tyr Tyr Glu Gly Lys Asn Ile Leu Leu Thr         
1               5                   10                  15              

ggt gca act gga ttt ctt ggg aag gtt ttg ctt gag aag ctg cta aga       96
Gly Ala Thr Gly Phe Leu Gly Lys Val Leu Leu Glu Lys Leu Leu Arg         
            20                  25                  30                  

tct tgc cct aga gtc aac tcc gtt tac gtt cta gtc aga caa aag gct      144
Ser Cys Pro Arg Val Asn Ser Val Tyr Val Leu Val Arg Gln Lys Ala         
        35                  40                  45                      

gga caa aca cct caa gaa aga gtt gag gag atc ttg tca agc aag ctc      192
Gly Gln Thr Pro Gln Glu Arg Val Glu Glu Ile Leu Ser Ser Lys Leu         
    50                  55                  60                          

ttt gat aga ctg cgt gat gag aat cca gat ttc cga gag aag atc atc      240
Phe Asp Arg Leu Arg Asp Glu Asn Pro Asp Phe Arg Glu Lys Ile Ile         
65                  70                  75                  80          

gcc atc aac tct gaa ttg acc caa cct aag tta gcg ctt tct gaa gag      288
Ala Ile Asn Ser Glu Leu Thr Gln Pro Lys Leu Ala Leu Ser Glu Glu         
                85                  90                  95              

gat aag gag atc atc atc gac tca acg aac gtt atc ttc cat tgc gct      336
Asp Lys Glu Ile Ile Ile Asp Ser Thr Asn Val Ile Phe His Cys Ala         
            100                 105                 110                 

gca aca gtt cgt ttc aat gag aac cta cgg gat gct gtt caa ttg aac      384
Ala Thr Val Arg Phe Asn Glu Asn Leu Arg Asp Ala Val Gln Leu Asn         
        115                 120                 125                     

gtc att gct acg aga caa ctg atc tta ctc gct cag cag atg aag aac      432
Val Ile Ala Thr Arg Gln Leu Ile Leu Leu Ala Gln Gln Met Lys Asn         
    130                 135                 140                         

ctc gaa gtc ttc atg cac gtt agt act gct tac gca tac tgt aac cgc      480
Leu Glu Val Phe Met His Val Ser Thr Ala Tyr Ala Tyr Cys Asn Arg         
145                 150                 155                 160         

aag cac atc gat gaa gtt gtt tac cca cct cca gtt gat cct aag aag      528
Lys His Ile Asp Glu Val Val Tyr Pro Pro Pro Val Asp Pro Lys Lys         
                165                 170                 175             

ttg atc gac tct ctt gag tgg atg gat gat gga ctt gtg aac gac ata      576
Leu Ile Asp Ser Leu Glu Trp Met Asp Asp Gly Leu Val Asn Asp Ile         
            180                 185                 190                 

aca cca aag ctt atc gga gac aga cca aac act tac atc tac act aag      624
Thr Pro Lys Leu Ile Gly Asp Arg Pro Asn Thr Tyr Ile Tyr Thr Lys         
        195                 200                 205                     

gca ctg gct gag tat gtt gtt caa caa gag gga gct aag ttg aac gtt      672
Ala Leu Ala Glu Tyr Val Val Gln Gln Glu Gly Ala Lys Leu Asn Val         
    210                 215                 220                         

gca atc gtt aga ccg tct att gtt gga gct tca tgg aaa gaa cca ttc      720
Ala Ile Val Arg Pro Ser Ile Val Gly Ala Ser Trp Lys Glu Pro Phe         
225                 230                 235                 240         

cca gga tgg att gac aac ttc aat ggt cca tct gga ctt ttc att gca      768
Pro Gly Trp Ile Asp Asn Phe Asn Gly Pro Ser Gly Leu Phe Ile Ala         
                245                 250                 255             

gct ggc aag ggt atc ctc aga act atg aga gca agt aac aac gca ctt      816
Ala Gly Lys Gly Ile Leu Arg Thr Met Arg Ala Ser Asn Asn Ala Leu         
            260                 265                 270                 

gca gat ctt gtt cct gtt gac gtt gtc gtt aac acc tca tta gca gct      864
Ala Asp Leu Val Pro Val Asp Val Val Val Asn Thr Ser Leu Ala Ala         
        275                 280                 285                     

gca tgg tat tct gga gtt aac aga ccc agg aac atc atg gtg tac aac      912
Ala Trp Tyr Ser Gly Val Asn Arg Pro Arg Asn Ile Met Val Tyr Asn         
    290                 295                 300                         

tgt aca acg gga tct aca aac cct ttt cat tgg ggt gaa gtt gag tat      960
Cys Thr Thr Gly Ser Thr Asn Pro Phe His Trp Gly Glu Val Glu Tyr         
305                 310                 315                 320         

cac gtc atc tct acc ttc aag aga aac cct ctt gag caa gct ttc aga     1008
His Val Ile Ser Thr Phe Lys Arg Asn Pro Leu Glu Gln Ala Phe Arg         
                325                 330                 335             

aga cct aac gtg aac ctt acc tcc aat cat cta ctc tac cac tac tgg     1056
Arg Pro Asn Val Asn Leu Thr Ser Asn His Leu Leu Tyr His Tyr Trp         
            340                 345                 350                 

att gct gtt tct cat aag gct cct gct ttc ttg tac gac atc tac ctt     1104
Ile Ala Val Ser His Lys Ala Pro Ala Phe Leu Tyr Asp Ile Tyr Leu         
        355                 360                 365                     

cga atg act ggt aga agt cct cgg atg atg aag acc att aca cga cta     1152
Arg Met Thr Gly Arg Ser Pro Arg Met Met Lys Thr Ile Thr Arg Leu         
    370                 375                 380                         

cac aag gct atg gtc ttc ctt gag tac ttc acc tca aac tca tgg gtt     1200
His Lys Ala Met Val Phe Leu Glu Tyr Phe Thr Ser Asn Ser Trp Val         
385                 390                 395                 400         

tgg aac act gac aac gtt aac atg ctc atg aac cag ctt aac cct gag     1248
Trp Asn Thr Asp Asn Val Asn Met Leu Met Asn Gln Leu Asn Pro Glu         
                405                 410                 415             

gac aag aag acg ttc aac att gat gtt cga cag ctt cac tgg gct gag     1296
Asp Lys Lys Thr Phe Asn Ile Asp Val Arg Gln Leu His Trp Ala Glu         
            420                 425                 430                 

tac ata gag aac tac tgt atg ggg acg aag aag tac gtt ctt aac gaa     1344
Tyr Ile Glu Asn Tyr Cys Met Gly Thr Lys Lys Tyr Val Leu Asn Glu         
        435                 440                 445                     

gag atg tct gga ctt cca gca gct aga aaa cat ctg aac aag ctt cgc     1392
Glu Met Ser Gly Leu Pro Ala Ala Arg Lys His Leu Asn Lys Leu Arg         
    450                 455                 460                         

aac atc aga taa                                                     1404
Asn Ile Arg                                                             
465                                                                     


<210>  6
<211>  467
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  6

Met Val Ser Ile Pro Glu Tyr Tyr Glu Gly Lys Asn Ile Leu Leu Thr 
1               5                   10                  15      


Gly Ala Thr Gly Phe Leu Gly Lys Val Leu Leu Glu Lys Leu Leu Arg 
            20                  25                  30          


Ser Cys Pro Arg Val Asn Ser Val Tyr Val Leu Val Arg Gln Lys Ala 
        35                  40                  45              


Gly Gln Thr Pro Gln Glu Arg Val Glu Glu Ile Leu Ser Ser Lys Leu 
    50                  55                  60                  


Phe Asp Arg Leu Arg Asp Glu Asn Pro Asp Phe Arg Glu Lys Ile Ile 
65                  70                  75                  80  


Ala Ile Asn Ser Glu Leu Thr Gln Pro Lys Leu Ala Leu Ser Glu Glu 
                85                  90                  95      


Asp Lys Glu Ile Ile Ile Asp Ser Thr Asn Val Ile Phe His Cys Ala 
            100                 105                 110         


Ala Thr Val Arg Phe Asn Glu Asn Leu Arg Asp Ala Val Gln Leu Asn 
        115                 120                 125             


Val Ile Ala Thr Arg Gln Leu Ile Leu Leu Ala Gln Gln Met Lys Asn 
    130                 135                 140                 


Leu Glu Val Phe Met His Val Ser Thr Ala Tyr Ala Tyr Cys Asn Arg 
145                 150                 155                 160 


Lys His Ile Asp Glu Val Val Tyr Pro Pro Pro Val Asp Pro Lys Lys 
                165                 170                 175     


Leu Ile Asp Ser Leu Glu Trp Met Asp Asp Gly Leu Val Asn Asp Ile 
            180                 185                 190         


Thr Pro Lys Leu Ile Gly Asp Arg Pro Asn Thr Tyr Ile Tyr Thr Lys 
        195                 200                 205             


Ala Leu Ala Glu Tyr Val Val Gln Gln Glu Gly Ala Lys Leu Asn Val 
    210                 215                 220                 


Ala Ile Val Arg Pro Ser Ile Val Gly Ala Ser Trp Lys Glu Pro Phe 
225                 230                 235                 240 


Pro Gly Trp Ile Asp Asn Phe Asn Gly Pro Ser Gly Leu Phe Ile Ala 
                245                 250                 255     


Ala Gly Lys Gly Ile Leu Arg Thr Met Arg Ala Ser Asn Asn Ala Leu 
            260                 265                 270         


Ala Asp Leu Val Pro Val Asp Val Val Val Asn Thr Ser Leu Ala Ala 
        275                 280                 285             


Ala Trp Tyr Ser Gly Val Asn Arg Pro Arg Asn Ile Met Val Tyr Asn 
    290                 295                 300                 


Cys Thr Thr Gly Ser Thr Asn Pro Phe His Trp Gly Glu Val Glu Tyr 
305                 310                 315                 320 


His Val Ile Ser Thr Phe Lys Arg Asn Pro Leu Glu Gln Ala Phe Arg 
                325                 330                 335     


Arg Pro Asn Val Asn Leu Thr Ser Asn His Leu Leu Tyr His Tyr Trp 
            340                 345                 350         


Ile Ala Val Ser His Lys Ala Pro Ala Phe Leu Tyr Asp Ile Tyr Leu 
        355                 360                 365             


Arg Met Thr Gly Arg Ser Pro Arg Met Met Lys Thr Ile Thr Arg Leu 
    370                 375                 380                 


His Lys Ala Met Val Phe Leu Glu Tyr Phe Thr Ser Asn Ser Trp Val 
385                 390                 395                 400 


Trp Asn Thr Asp Asn Val Asn Met Leu Met Asn Gln Leu Asn Pro Glu 
                405                 410                 415     


Asp Lys Lys Thr Phe Asn Ile Asp Val Arg Gln Leu His Trp Ala Glu 
            420                 425                 430         


Tyr Ile Glu Asn Tyr Cys Met Gly Thr Lys Lys Tyr Val Leu Asn Glu 
        435                 440                 445             


Glu Met Ser Gly Leu Pro Ala Ala Arg Lys His Leu Asn Lys Leu Arg 
    450                 455                 460                 


Asn Ile Arg 
465         


<210>  7
<211>  2130
<212>  DNA
<213>  Artificial

<220>
<223>  mouse fatty acyl CoA reductase tagged with mCherry


<220>
<221>  CDS
<222>  (7)..(2124)

<400>  7
ggatcc atg gtg agc aag ggc gag gag gat aac atg gcc atc atc aag        48
       Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys          
       1               5                   10                           

gag ttc atg cgc ttc aag gtg cac atg gag ggc tcc gtg aac ggc cac       96
Glu Phe Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His         
15                  20                  25                  30          

gag ttc gag atc gag ggc gag ggc gag ggc cgc ccc tac gag ggc acc      144
Glu Phe Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr         
                35                  40                  45              

cag acc gcc aag ctg aag gtg acc aag ggt ggc ccc ctg ccc ttc gcc      192
Gln Thr Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala         
            50                  55                  60                  

tgg gac atc ctg tcc cct cag ttc atg tac ggc tcc aag gcc tac gtg      240
Trp Asp Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val         
        65                  70                  75                      

aag cac ccc gcc gac atc ccc gac tac ttg aag ctg tcc ttc ccc gag      288
Lys His Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu         
    80                  85                  90                          

ggc ttc aag tgg gag cgc gtg atg aac ttc gag gac ggc ggc gtg gtg      336
Gly Phe Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val         
95                  100                 105                 110         

acc gtg acc cag gac tcc tcc ctg cag gac ggc gag ttc atc tac aag      384
Thr Val Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys         
                115                 120                 125             

gtg aag ctg cgc ggc acc aac ttc ccc tcc gac ggc ccc gta atg cag      432
Val Lys Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln         
            130                 135                 140                 

aag aag acc atg ggc tgg gag gcc tcc tcc gag cgg atg tac ccc gag      480
Lys Lys Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu         
        145                 150                 155                     

gac ggc gcc ctg aag ggc gag atc aag cag agg ctg aag ctg aag gac      528
Asp Gly Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp         
    160                 165                 170                         

ggc ggc cac tac gac gct gag gtc aag acc acc tac aag gcc aag aag      576
Gly Gly His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys         
175                 180                 185                 190         

ccc gtg cag ctg ccc ggc gcc tac aac gtc aac atc aag ttg gac atc      624
Pro Val Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile         
                195                 200                 205             

acc tcc cac aac gag gac tac acc atc gtg gaa cag tac gaa cgc gcc      672
Thr Ser His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala         
            210                 215                 220                 

gag ggc cgc cac tcc acc ggc ggc atg gac gag ctg tac aag gaa ttc      720
Glu Gly Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys Glu Phe         
        225                 230                 235                     

atg gtt tcc atc cca gag tac tat gag gga aag aac atc ttg cta act      768
Met Val Ser Ile Pro Glu Tyr Tyr Glu Gly Lys Asn Ile Leu Leu Thr         
    240                 245                 250                         

ggt gca act gga ttt ctt ggg aag gtt ttg ctt gag aag ctg cta aga      816
Gly Ala Thr Gly Phe Leu Gly Lys Val Leu Leu Glu Lys Leu Leu Arg         
255                 260                 265                 270         

tct tgc cct aga gtc aac tcc gtt tac gtt cta gtc aga caa aag gct      864
Ser Cys Pro Arg Val Asn Ser Val Tyr Val Leu Val Arg Gln Lys Ala         
                275                 280                 285             

gga caa aca cct caa gaa aga gtt gag gag atc ttg tca agc aag ctc      912
Gly Gln Thr Pro Gln Glu Arg Val Glu Glu Ile Leu Ser Ser Lys Leu         
            290                 295                 300                 

ttt gat aga ctg cgt gat gag aat cca gat ttc cga gag aag atc atc      960
Phe Asp Arg Leu Arg Asp Glu Asn Pro Asp Phe Arg Glu Lys Ile Ile         
        305                 310                 315                     

gcc atc aac tct gaa ttg acc caa cct aag tta gcg ctt tct gaa gag     1008
Ala Ile Asn Ser Glu Leu Thr Gln Pro Lys Leu Ala Leu Ser Glu Glu         
    320                 325                 330                         

gat aag gag atc atc atc gac tca acg aac gtt atc ttc cat tgc gct     1056
Asp Lys Glu Ile Ile Ile Asp Ser Thr Asn Val Ile Phe His Cys Ala         
335                 340                 345                 350         

gca aca gtt cgt ttc aat gag aac cta cgg gat gct gtt caa ttg aac     1104
Ala Thr Val Arg Phe Asn Glu Asn Leu Arg Asp Ala Val Gln Leu Asn         
                355                 360                 365             

gtc att gct acg aga caa ctg atc tta ctc gct cag cag atg aag aac     1152
Val Ile Ala Thr Arg Gln Leu Ile Leu Leu Ala Gln Gln Met Lys Asn         
            370                 375                 380                 

ctc gaa gtc ttc atg cac gtt agt act gct tac gca tac tgt aac cgc     1200
Leu Glu Val Phe Met His Val Ser Thr Ala Tyr Ala Tyr Cys Asn Arg         
        385                 390                 395                     

aag cac atc gat gaa gtt gtt tac cca cct cca gtt gat cct aag aag     1248
Lys His Ile Asp Glu Val Val Tyr Pro Pro Pro Val Asp Pro Lys Lys         
    400                 405                 410                         

ttg atc gac tct ctt gag tgg atg gat gat gga ctt gtg aac gac ata     1296
Leu Ile Asp Ser Leu Glu Trp Met Asp Asp Gly Leu Val Asn Asp Ile         
415                 420                 425                 430         

aca cca aag ctt atc gga gac aga cca aac act tac atc tac act aag     1344
Thr Pro Lys Leu Ile Gly Asp Arg Pro Asn Thr Tyr Ile Tyr Thr Lys         
                435                 440                 445             

gca ctg gct gag tat gtt gtt caa caa gag gga gct aag ttg aac gtt     1392
Ala Leu Ala Glu Tyr Val Val Gln Gln Glu Gly Ala Lys Leu Asn Val         
            450                 455                 460                 

gca atc gtt aga ccg tct att gtt gga gct tca tgg aaa gaa cca ttc     1440
Ala Ile Val Arg Pro Ser Ile Val Gly Ala Ser Trp Lys Glu Pro Phe         
        465                 470                 475                     

cca gga tgg att gac aac ttc aat ggt cca tct gga ctt ttc att gca     1488
Pro Gly Trp Ile Asp Asn Phe Asn Gly Pro Ser Gly Leu Phe Ile Ala         
    480                 485                 490                         

gct ggc aag ggt atc ctc aga act atg aga gca agt aac aac gca ctt     1536
Ala Gly Lys Gly Ile Leu Arg Thr Met Arg Ala Ser Asn Asn Ala Leu         
495                 500                 505                 510         

gca gat ctt gtt cct gtt gac gtt gtc gtt aac acc tca tta gca gct     1584
Ala Asp Leu Val Pro Val Asp Val Val Val Asn Thr Ser Leu Ala Ala         
                515                 520                 525             

gca tgg tat tct gga gtt aac aga ccc agg aac atc atg gtg tac aac     1632
Ala Trp Tyr Ser Gly Val Asn Arg Pro Arg Asn Ile Met Val Tyr Asn         
            530                 535                 540                 

tgt aca acg gga tct aca aac cct ttt cat tgg ggt gaa gtt gag tat     1680
Cys Thr Thr Gly Ser Thr Asn Pro Phe His Trp Gly Glu Val Glu Tyr         
        545                 550                 555                     

cac gtc atc tct acc ttc aag aga aac cct ctt gag caa gct ttc aga     1728
His Val Ile Ser Thr Phe Lys Arg Asn Pro Leu Glu Gln Ala Phe Arg         
    560                 565                 570                         

aga cct aac gtg aac ctt acc tcc aat cat cta ctc tac cac tac tgg     1776
Arg Pro Asn Val Asn Leu Thr Ser Asn His Leu Leu Tyr His Tyr Trp         
575                 580                 585                 590         

att gct gtt tct cat aag gct cct gct ttc ttg tac gac atc tac ctt     1824
Ile Ala Val Ser His Lys Ala Pro Ala Phe Leu Tyr Asp Ile Tyr Leu         
                595                 600                 605             

cga atg act ggt aga agt cct cgg atg atg aag acc att aca cga cta     1872
Arg Met Thr Gly Arg Ser Pro Arg Met Met Lys Thr Ile Thr Arg Leu         
            610                 615                 620                 

cac aag gct atg gtc ttc ctt gag tac ttc acc tca aac tca tgg gtt     1920
His Lys Ala Met Val Phe Leu Glu Tyr Phe Thr Ser Asn Ser Trp Val         
        625                 630                 635                     

tgg aac act gac aac gtt aac atg ctc atg aac cag ctt aac cct gag     1968
Trp Asn Thr Asp Asn Val Asn Met Leu Met Asn Gln Leu Asn Pro Glu         
    640                 645                 650                         

gac aag aag acg ttc aac att gat gtt cga cag ctt cac tgg gct gag     2016
Asp Lys Lys Thr Phe Asn Ile Asp Val Arg Gln Leu His Trp Ala Glu         
655                 660                 665                 670         

tac ata gag aac tac tgt atg ggg acg aag aag tac gtt ctt aac gaa     2064
Tyr Ile Glu Asn Tyr Cys Met Gly Thr Lys Lys Tyr Val Leu Asn Glu         
                675                 680                 685             

gag atg tct gga ctt cca gca gct aga aaa cat ctg aac aag ctt cgc     2112
Glu Met Ser Gly Leu Pro Ala Ala Arg Lys His Leu Asn Lys Leu Arg         
            690                 695                 700                 

aac atc aga taa ctcgag                                              2130
Asn Ile Arg                                                             
        705                                                             


<210>  8
<211>  705
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  8

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe 
1               5                   10                  15      


Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe 
            20                  25                  30          


Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
        35                  40                  45              


Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
    50                  55                  60                  


Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His 
65                  70                  75                  80  


Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
                85                  90                  95      


Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
            100                 105                 110         


Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
        115                 120                 125             


Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly 
145                 150                 155                 160 


Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly 
                165                 170                 175     


His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val 
            180                 185                 190         


Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser 
        195                 200                 205             


His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
    210                 215                 220                 


Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys Glu Phe Met Val 
225                 230                 235                 240 


Ser Ile Pro Glu Tyr Tyr Glu Gly Lys Asn Ile Leu Leu Thr Gly Ala 
                245                 250                 255     


Thr Gly Phe Leu Gly Lys Val Leu Leu Glu Lys Leu Leu Arg Ser Cys 
            260                 265                 270         


Pro Arg Val Asn Ser Val Tyr Val Leu Val Arg Gln Lys Ala Gly Gln 
        275                 280                 285             


Thr Pro Gln Glu Arg Val Glu Glu Ile Leu Ser Ser Lys Leu Phe Asp 
    290                 295                 300                 


Arg Leu Arg Asp Glu Asn Pro Asp Phe Arg Glu Lys Ile Ile Ala Ile 
305                 310                 315                 320 


Asn Ser Glu Leu Thr Gln Pro Lys Leu Ala Leu Ser Glu Glu Asp Lys 
                325                 330                 335     


Glu Ile Ile Ile Asp Ser Thr Asn Val Ile Phe His Cys Ala Ala Thr 
            340                 345                 350         


Val Arg Phe Asn Glu Asn Leu Arg Asp Ala Val Gln Leu Asn Val Ile 
        355                 360                 365             


Ala Thr Arg Gln Leu Ile Leu Leu Ala Gln Gln Met Lys Asn Leu Glu 
    370                 375                 380                 


Val Phe Met His Val Ser Thr Ala Tyr Ala Tyr Cys Asn Arg Lys His 
385                 390                 395                 400 


Ile Asp Glu Val Val Tyr Pro Pro Pro Val Asp Pro Lys Lys Leu Ile 
                405                 410                 415     


Asp Ser Leu Glu Trp Met Asp Asp Gly Leu Val Asn Asp Ile Thr Pro 
            420                 425                 430         


Lys Leu Ile Gly Asp Arg Pro Asn Thr Tyr Ile Tyr Thr Lys Ala Leu 
        435                 440                 445             


Ala Glu Tyr Val Val Gln Gln Glu Gly Ala Lys Leu Asn Val Ala Ile 
    450                 455                 460                 


Val Arg Pro Ser Ile Val Gly Ala Ser Trp Lys Glu Pro Phe Pro Gly 
465                 470                 475                 480 


Trp Ile Asp Asn Phe Asn Gly Pro Ser Gly Leu Phe Ile Ala Ala Gly 
                485                 490                 495     


Lys Gly Ile Leu Arg Thr Met Arg Ala Ser Asn Asn Ala Leu Ala Asp 
            500                 505                 510         


Leu Val Pro Val Asp Val Val Val Asn Thr Ser Leu Ala Ala Ala Trp 
        515                 520                 525             


Tyr Ser Gly Val Asn Arg Pro Arg Asn Ile Met Val Tyr Asn Cys Thr 
    530                 535                 540                 


Thr Gly Ser Thr Asn Pro Phe His Trp Gly Glu Val Glu Tyr His Val 
545                 550                 555                 560 


Ile Ser Thr Phe Lys Arg Asn Pro Leu Glu Gln Ala Phe Arg Arg Pro 
                565                 570                 575     


Asn Val Asn Leu Thr Ser Asn His Leu Leu Tyr His Tyr Trp Ile Ala 
            580                 585                 590         


Val Ser His Lys Ala Pro Ala Phe Leu Tyr Asp Ile Tyr Leu Arg Met 
        595                 600                 605             


Thr Gly Arg Ser Pro Arg Met Met Lys Thr Ile Thr Arg Leu His Lys 
    610                 615                 620                 


Ala Met Val Phe Leu Glu Tyr Phe Thr Ser Asn Ser Trp Val Trp Asn 
625                 630                 635                 640 


Thr Asp Asn Val Asn Met Leu Met Asn Gln Leu Asn Pro Glu Asp Lys 
                645                 650                 655     


Lys Thr Phe Asn Ile Asp Val Arg Gln Leu His Trp Ala Glu Tyr Ile 
            660                 665                 670         


Glu Asn Tyr Cys Met Gly Thr Lys Lys Tyr Val Leu Asn Glu Glu Met 
        675                 680                 685             


Ser Gly Leu Pro Ala Ala Arg Lys His Leu Asn Lys Leu Arg Asn Ile 
    690                 695                 700                 


Arg 
705 


<210>  9
<211>  2559
<212>  DNA
<213>  Artificial

<220>
<223>  mouse fatty acyl CoA reductase truncated, tagged with mCherry and
       fused to oleosin 3At


<220>
<221>  CDS
<222>  (7)..(2553)

<400>  9
gtcgac atg gcg gac caa aca aga acc cat cac gag atg ata agc cga        48
       Met Ala Asp Gln Thr Arg Thr His His Glu Met Ile Ser Arg          
       1               5                   10                           

gac agt acc caa gag gcc cat ccg aag gcc agg cag atg gtg aag gca       96
Asp Ser Thr Gln Glu Ala His Pro Lys Ala Arg Gln Met Val Lys Ala         
15                  20                  25                  30          

gca acc gct gtc aca gcc ggt gga tcc cta ctt gtc ctc tcc ggc tta      144
Ala Thr Ala Val Thr Ala Gly Gly Ser Leu Leu Val Leu Ser Gly Leu         
                35                  40                  45              

aca ctc gct gga aca gtc atc gca ctc acg gtg gct act cct ctc ctc      192
Thr Leu Ala Gly Thr Val Ile Ala Leu Thr Val Ala Thr Pro Leu Leu         
            50                  55                  60                  

gtc atc ttc agc ccc gtc ttg gtt cca gca gtg gta acc gtt gct ctc      240
Val Ile Phe Ser Pro Val Leu Val Pro Ala Val Val Thr Val Ala Leu         
        65                  70                  75                      

atc att acc gga ttc ctt gca tcc ggt ggc ttt gga ata gcc gcc att      288
Ile Ile Thr Gly Phe Leu Ala Ser Gly Gly Phe Gly Ile Ala Ala Ile         
    80                  85                  90                          

acc gcc ttc tct tgg ctc tac agg cac atg acg gga tct gga tcg gat      336
Thr Ala Phe Ser Trp Leu Tyr Arg His Met Thr Gly Ser Gly Ser Asp         
95                  100                 105                 110         

aag ata gag aat gct cgg atg aag gtt gga agc aga gtg cag gat act      384
Lys Ile Glu Asn Ala Arg Met Lys Val Gly Ser Arg Val Gln Asp Thr         
                115                 120                 125             

aag tat ggg cag cac aac att gga gtc caa cac caa caa gtt tct gga      432
Lys Tyr Gly Gln His Asn Ile Gly Val Gln His Gln Gln Val Ser Gly         
            130                 135                 140                 

tcc atg gtg agc aag ggc gag gag gat aac atg gcc atc atc aag gag      480
Ser Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu         
        145                 150                 155                     

ttc atg cgc ttc aag gtg cac atg gag ggc tcc gtg aac ggc cac gag      528
Phe Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu         
    160                 165                 170                         

ttc gag atc gag ggc gag ggc gag ggc cgc ccc tac gag ggc acc cag      576
Phe Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln         
175                 180                 185                 190         

acc gcc aag ctg aag gtg acc aag ggt ggc ccc ctg ccc ttc gcc tgg      624
Thr Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp         
                195                 200                 205             

gac atc ctg tcc cct cag ttc atg tac ggc tcc aag gcc tac gtg aag      672
Asp Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys         
            210                 215                 220                 

cac ccc gcc gac atc ccc gac tac ttg aag ctg tcc ttc ccc gag ggc      720
His Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly         
        225                 230                 235                     

ttc aag tgg gag cgc gtg atg aac ttc gag gac ggc ggc gtg gtg acc      768
Phe Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr         
    240                 245                 250                         

gtg acc cag gac tcc tcc ctg cag gac ggc gag ttc atc tac aag gtg      816
Val Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val         
255                 260                 265                 270         

aag ctg cgc ggc acc aac ttc ccc tcc gac ggc ccc gta atg cag aag      864
Lys Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys         
                275                 280                 285             

aag acc atg ggc tgg gag gcc tcc tcc gag cgg atg tac ccc gag gac      912
Lys Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp         
            290                 295                 300                 

ggc gcc ctg aag ggc gag atc aag cag agg ctg aag ctg aag gac ggc      960
Gly Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly         
        305                 310                 315                     

ggc cac tac gac gct gag gtc aag acc acc tac aag gcc aag aag ccc     1008
Gly His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro         
    320                 325                 330                         

gtg cag ctg ccc ggc gcc tac aac gtc aac atc aag ttg gac atc acc     1056
Val Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr         
335                 340                 345                 350         

tcc cac aac gag gac tac acc atc gtg gaa cag tac gaa cgc gcc gag     1104
Ser His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu         
                355                 360                 365             

ggc cgc cac tcc acc ggc ggc atg gac gag ctg tac aag gaa ttc atg     1152
Gly Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys Glu Phe Met         
            370                 375                 380                 

gtt tcc atc cca gag tac tat gag gga aag aac atc ttg cta act ggt     1200
Val Ser Ile Pro Glu Tyr Tyr Glu Gly Lys Asn Ile Leu Leu Thr Gly         
        385                 390                 395                     

gca act gga ttt ctt ggg aag gtt ttg ctt gag aag ctg cta aga tct     1248
Ala Thr Gly Phe Leu Gly Lys Val Leu Leu Glu Lys Leu Leu Arg Ser         
    400                 405                 410                         

tgc cct aga gtc aac tcc gtt tac gtt cta gtc aga caa aag gct gga     1296
Cys Pro Arg Val Asn Ser Val Tyr Val Leu Val Arg Gln Lys Ala Gly         
415                 420                 425                 430         

caa aca cct caa gaa aga gtt gag gag atc ttg tca agc aag ctc ttt     1344
Gln Thr Pro Gln Glu Arg Val Glu Glu Ile Leu Ser Ser Lys Leu Phe         
                435                 440                 445             

gat aga ctg cgt gat gag aat cca gat ttc cga gag aag atc atc gcc     1392
Asp Arg Leu Arg Asp Glu Asn Pro Asp Phe Arg Glu Lys Ile Ile Ala         
            450                 455                 460                 

atc aac tct gaa ttg acc caa cct aag tta gcg ctt tct gaa gag gat     1440
Ile Asn Ser Glu Leu Thr Gln Pro Lys Leu Ala Leu Ser Glu Glu Asp         
        465                 470                 475                     

aag gag atc atc atc gac tca acg aac gtt atc ttc cat tgc gct gca     1488
Lys Glu Ile Ile Ile Asp Ser Thr Asn Val Ile Phe His Cys Ala Ala         
    480                 485                 490                         

aca gtt cgt ttc aat gag aac cta cgg gat gct gtt caa ttg aac gtc     1536
Thr Val Arg Phe Asn Glu Asn Leu Arg Asp Ala Val Gln Leu Asn Val         
495                 500                 505                 510         

att gct acg aga caa ctg atc tta ctc gct cag cag atg aag aac ctc     1584
Ile Ala Thr Arg Gln Leu Ile Leu Leu Ala Gln Gln Met Lys Asn Leu         
                515                 520                 525             

gaa gtc ttc atg cac gtt agt act gct tac gca tac tgt aac cgc aag     1632
Glu Val Phe Met His Val Ser Thr Ala Tyr Ala Tyr Cys Asn Arg Lys         
            530                 535                 540                 

cac atc gat gaa gtt gtt tac cca cct cca gtt gat cct aag aag ttg     1680
His Ile Asp Glu Val Val Tyr Pro Pro Pro Val Asp Pro Lys Lys Leu         
        545                 550                 555                     

atc gac tct ctt gag tgg atg gat gat gga ctt gtg aac gac ata aca     1728
Ile Asp Ser Leu Glu Trp Met Asp Asp Gly Leu Val Asn Asp Ile Thr         
    560                 565                 570                         

cca aag ctt atc gga gac aga cca aac act tac atc tac act aag gca     1776
Pro Lys Leu Ile Gly Asp Arg Pro Asn Thr Tyr Ile Tyr Thr Lys Ala         
575                 580                 585                 590         

ctg gct gag tat gtt gtt caa caa gag gga gct aag ttg aac gtt gca     1824
Leu Ala Glu Tyr Val Val Gln Gln Glu Gly Ala Lys Leu Asn Val Ala         
                595                 600                 605             

atc gtt aga ccg tct att gtt gga gct tca tgg aaa gaa cca ttc cca     1872
Ile Val Arg Pro Ser Ile Val Gly Ala Ser Trp Lys Glu Pro Phe Pro         
            610                 615                 620                 

gga tgg att gac aac ttc aat ggt cca tct gga ctt ttc att gca gct     1920
Gly Trp Ile Asp Asn Phe Asn Gly Pro Ser Gly Leu Phe Ile Ala Ala         
        625                 630                 635                     

ggc aag ggt atc ctc aga act atg aga gca agt aac aac gca ctt gca     1968
Gly Lys Gly Ile Leu Arg Thr Met Arg Ala Ser Asn Asn Ala Leu Ala         
    640                 645                 650                         

gat ctt gtt cct gtt gac gtt gtc gtt aac acc tca tta gca gct gca     2016
Asp Leu Val Pro Val Asp Val Val Val Asn Thr Ser Leu Ala Ala Ala         
655                 660                 665                 670         

tgg tat tct gga gtt aac aga ccc agg aac atc atg gtg tac aac tgt     2064
Trp Tyr Ser Gly Val Asn Arg Pro Arg Asn Ile Met Val Tyr Asn Cys         
                675                 680                 685             

aca acg gga tct aca aac cct ttt cat tgg ggt gaa gtt gag tat cac     2112
Thr Thr Gly Ser Thr Asn Pro Phe His Trp Gly Glu Val Glu Tyr His         
            690                 695                 700                 

gtc atc tct acc ttc aag aga aac cct ctt gag caa gct ttc aga aga     2160
Val Ile Ser Thr Phe Lys Arg Asn Pro Leu Glu Gln Ala Phe Arg Arg         
        705                 710                 715                     

cct aac gtg aac ctt acc tcc aat cat cta ctc tac cac tac tgg att     2208
Pro Asn Val Asn Leu Thr Ser Asn His Leu Leu Tyr His Tyr Trp Ile         
    720                 725                 730                         

gct gtt tct cat aag gct cct gct ttc ttg tac gac atc tac ctt cga     2256
Ala Val Ser His Lys Ala Pro Ala Phe Leu Tyr Asp Ile Tyr Leu Arg         
735                 740                 745                 750         

atg act ggt aga agt cct cgg atg atg aag acc att aca cga cta cac     2304
Met Thr Gly Arg Ser Pro Arg Met Met Lys Thr Ile Thr Arg Leu His         
                755                 760                 765             

aag gct atg gtc ttc ctt gag tac ttc acc tca aac tca tgg gtt tgg     2352
Lys Ala Met Val Phe Leu Glu Tyr Phe Thr Ser Asn Ser Trp Val Trp         
            770                 775                 780                 

aac act gac aac gtt aac atg ctc atg aac cag ctt aac cct gag gac     2400
Asn Thr Asp Asn Val Asn Met Leu Met Asn Gln Leu Asn Pro Glu Asp         
        785                 790                 795                     

aag aag acg ttc aac att gat gtt cga cag ctt cac tgg gct gag tac     2448
Lys Lys Thr Phe Asn Ile Asp Val Arg Gln Leu His Trp Ala Glu Tyr         
    800                 805                 810                         

ata gag aac tac tgt atg ggg acg aag aag tac gtt ctt aac gaa gag     2496
Ile Glu Asn Tyr Cys Met Gly Thr Lys Lys Tyr Val Leu Asn Glu Glu         
815                 820                 825                 830         

atg tct gga ctt cca gca gct aga aaa cat ctg aac aag ctt cgc aac     2544
Met Ser Gly Leu Pro Ala Ala Arg Lys His Leu Asn Lys Leu Arg Asn         
                835                 840                 845             

atc aga taa ctcgag                                                  2559
Ile Arg                                                                 
                                                                        


<210>  10
<211>  848
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  10

Met Ala Asp Gln Thr Arg Thr His His Glu Met Ile Ser Arg Asp Ser 
1               5                   10                  15      


Thr Gln Glu Ala His Pro Lys Ala Arg Gln Met Val Lys Ala Ala Thr 
            20                  25                  30          


Ala Val Thr Ala Gly Gly Ser Leu Leu Val Leu Ser Gly Leu Thr Leu 
        35                  40                  45              


Ala Gly Thr Val Ile Ala Leu Thr Val Ala Thr Pro Leu Leu Val Ile 
    50                  55                  60                  


Phe Ser Pro Val Leu Val Pro Ala Val Val Thr Val Ala Leu Ile Ile 
65                  70                  75                  80  


Thr Gly Phe Leu Ala Ser Gly Gly Phe Gly Ile Ala Ala Ile Thr Ala 
                85                  90                  95      


Phe Ser Trp Leu Tyr Arg His Met Thr Gly Ser Gly Ser Asp Lys Ile 
            100                 105                 110         


Glu Asn Ala Arg Met Lys Val Gly Ser Arg Val Gln Asp Thr Lys Tyr 
        115                 120                 125             


Gly Gln His Asn Ile Gly Val Gln His Gln Gln Val Ser Gly Ser Met 
    130                 135                 140                 


Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met 
145                 150                 155                 160 


Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu 
                165                 170                 175     


Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala 
            180                 185                 190         


Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile 
        195                 200                 205             


Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro 
    210                 215                 220                 


Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys 
225                 230                 235                 240 


Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr 
                245                 250                 255     


Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu 
            260                 265                 270         


Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr 
        275                 280                 285             


Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala 
    290                 295                 300                 


Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His 
305                 310                 315                 320 


Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln 
                325                 330                 335     


Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser His 
            340                 345                 350         


Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg 
        355                 360                 365             


His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys Glu Phe Met Val Ser 
    370                 375                 380                 


Ile Pro Glu Tyr Tyr Glu Gly Lys Asn Ile Leu Leu Thr Gly Ala Thr 
385                 390                 395                 400 


Gly Phe Leu Gly Lys Val Leu Leu Glu Lys Leu Leu Arg Ser Cys Pro 
                405                 410                 415     


Arg Val Asn Ser Val Tyr Val Leu Val Arg Gln Lys Ala Gly Gln Thr 
            420                 425                 430         


Pro Gln Glu Arg Val Glu Glu Ile Leu Ser Ser Lys Leu Phe Asp Arg 
        435                 440                 445             


Leu Arg Asp Glu Asn Pro Asp Phe Arg Glu Lys Ile Ile Ala Ile Asn 
    450                 455                 460                 


Ser Glu Leu Thr Gln Pro Lys Leu Ala Leu Ser Glu Glu Asp Lys Glu 
465                 470                 475                 480 


Ile Ile Ile Asp Ser Thr Asn Val Ile Phe His Cys Ala Ala Thr Val 
                485                 490                 495     


Arg Phe Asn Glu Asn Leu Arg Asp Ala Val Gln Leu Asn Val Ile Ala 
            500                 505                 510         


Thr Arg Gln Leu Ile Leu Leu Ala Gln Gln Met Lys Asn Leu Glu Val 
        515                 520                 525             


Phe Met His Val Ser Thr Ala Tyr Ala Tyr Cys Asn Arg Lys His Ile 
    530                 535                 540                 


Asp Glu Val Val Tyr Pro Pro Pro Val Asp Pro Lys Lys Leu Ile Asp 
545                 550                 555                 560 


Ser Leu Glu Trp Met Asp Asp Gly Leu Val Asn Asp Ile Thr Pro Lys 
                565                 570                 575     


Leu Ile Gly Asp Arg Pro Asn Thr Tyr Ile Tyr Thr Lys Ala Leu Ala 
            580                 585                 590         


Glu Tyr Val Val Gln Gln Glu Gly Ala Lys Leu Asn Val Ala Ile Val 
        595                 600                 605             


Arg Pro Ser Ile Val Gly Ala Ser Trp Lys Glu Pro Phe Pro Gly Trp 
    610                 615                 620                 


Ile Asp Asn Phe Asn Gly Pro Ser Gly Leu Phe Ile Ala Ala Gly Lys 
625                 630                 635                 640 


Gly Ile Leu Arg Thr Met Arg Ala Ser Asn Asn Ala Leu Ala Asp Leu 
                645                 650                 655     


Val Pro Val Asp Val Val Val Asn Thr Ser Leu Ala Ala Ala Trp Tyr 
            660                 665                 670         


Ser Gly Val Asn Arg Pro Arg Asn Ile Met Val Tyr Asn Cys Thr Thr 
        675                 680                 685             


Gly Ser Thr Asn Pro Phe His Trp Gly Glu Val Glu Tyr His Val Ile 
    690                 695                 700                 


Ser Thr Phe Lys Arg Asn Pro Leu Glu Gln Ala Phe Arg Arg Pro Asn 
705                 710                 715                 720 


Val Asn Leu Thr Ser Asn His Leu Leu Tyr His Tyr Trp Ile Ala Val 
                725                 730                 735     


Ser His Lys Ala Pro Ala Phe Leu Tyr Asp Ile Tyr Leu Arg Met Thr 
            740                 745                 750         


Gly Arg Ser Pro Arg Met Met Lys Thr Ile Thr Arg Leu His Lys Ala 
        755                 760                 765             


Met Val Phe Leu Glu Tyr Phe Thr Ser Asn Ser Trp Val Trp Asn Thr 
    770                 775                 780                 


Asp Asn Val Asn Met Leu Met Asn Gln Leu Asn Pro Glu Asp Lys Lys 
785                 790                 795                 800 


Thr Phe Asn Ile Asp Val Arg Gln Leu His Trp Ala Glu Tyr Ile Glu 
                805                 810                 815     


Asn Tyr Cys Met Gly Thr Lys Lys Tyr Val Leu Asn Glu Glu Met Ser 
            820                 825                 830         


Gly Leu Pro Ala Ala Arg Lys His Leu Asn Lys Leu Arg Asn Ile Arg 
        835                 840                 845             


<210>  11
<211>  1002
<212>  DNA
<213>  Artificial

<220>
<223>  Wax ester synthase from mouse


<220>
<221>  CDS
<222>  (1)..(1002)

<400>  11
atg ttc tgg cct act aag aag gac ctt aag act gca atg gag gtt ttc       48
Met Phe Trp Pro Thr Lys Lys Asp Leu Lys Thr Ala Met Glu Val Phe         
1               5                   10                  15              

gct ctt ttc caa tgg gct ttg tct gca ctc gtt atc gtt act acc gtc       96
Ala Leu Phe Gln Trp Ala Leu Ser Ala Leu Val Ile Val Thr Thr Val         
            20                  25                  30                  

atc atc gtg aac ctg tac ctt gtt gtg ttc act tca tac tgg cca gtt      144
Ile Ile Val Asn Leu Tyr Leu Val Val Phe Thr Ser Tyr Trp Pro Val         
        35                  40                  45                      

acc gtt ttg atg cta aca tgg ctg gca ttc gat tgg aaa aca cct gaa      192
Thr Val Leu Met Leu Thr Trp Leu Ala Phe Asp Trp Lys Thr Pro Glu         
    50                  55                  60                          

aga gga ggt aga agg ttc aca tgt gtg aga aag tgg cgt ctt tgg aaa      240
Arg Gly Gly Arg Arg Phe Thr Cys Val Arg Lys Trp Arg Leu Trp Lys         
65                  70                  75                  80          

cac tac tct gac tac ttc cct ctt aag atg gtg aag acg aag gat atc      288
His Tyr Ser Asp Tyr Phe Pro Leu Lys Met Val Lys Thr Lys Asp Ile         
                85                  90                  95              

tcc cct gat aga aac tac ata ctt gtc tgt cac cca cat ggt ctt atg      336
Ser Pro Asp Arg Asn Tyr Ile Leu Val Cys His Pro His Gly Leu Met         
            100                 105                 110                 

gca cat agt tgt ttc gga cat ttc gct acc gat act aca gga ttc agt      384
Ala His Ser Cys Phe Gly His Phe Ala Thr Asp Thr Thr Gly Phe Ser         
        115                 120                 125                     

aag acc ttc cct ggt atc aca cct tac atg ctt act ctt gga gct ttc      432
Lys Thr Phe Pro Gly Ile Thr Pro Tyr Met Leu Thr Leu Gly Ala Phe         
    130                 135                 140                         

ttc tgg gtt cct ttc ttg aga gat tac gtc atg tca acc gga agt tgt      480
Phe Trp Val Pro Phe Leu Arg Asp Tyr Val Met Ser Thr Gly Ser Cys         
145                 150                 155                 160         

tct gtg tcc aga agc tct atg gac ttc ttg ctt acg caa aag ggt aca      528
Ser Val Ser Arg Ser Ser Met Asp Phe Leu Leu Thr Gln Lys Gly Thr         
                165                 170                 175             

gga aac atg ctt gtt gtc gtt gtt ggt gga ctt gct gaa tgt aga tac      576
Gly Asn Met Leu Val Val Val Val Gly Gly Leu Ala Glu Cys Arg Tyr         
            180                 185                 190                 

tct aca cca ggc tca aca acc ctt ttc cta aag aag agg cag gga ttt      624
Ser Thr Pro Gly Ser Thr Thr Leu Phe Leu Lys Lys Arg Gln Gly Phe         
        195                 200                 205                     

gtt cgt aca gca cta aag cat ggg gtt tct ttg atc cct gct tat gca      672
Val Arg Thr Ala Leu Lys His Gly Val Ser Leu Ile Pro Ala Tyr Ala         
    210                 215                 220                         

ttt gga gag act gac ctc tac gat caa cat atc ttc act cct gga gga      720
Phe Gly Glu Thr Asp Leu Tyr Asp Gln His Ile Phe Thr Pro Gly Gly         
225                 230                 235                 240         

ttc gtc aac aga ttc caa aag tgg ttc cag aag atg gtt cac atc tac      768
Phe Val Asn Arg Phe Gln Lys Trp Phe Gln Lys Met Val His Ile Tyr         
                245                 250                 255             

cca tgt gct ttc tat gga aga ggt ctg acc aag aat tca tgg ggt ctt      816
Pro Cys Ala Phe Tyr Gly Arg Gly Leu Thr Lys Asn Ser Trp Gly Leu         
            260                 265                 270                 

ttg cct tac tca caa cca gtt act aca gtt gtt gga gag cca tta ccg      864
Leu Pro Tyr Ser Gln Pro Val Thr Thr Val Val Gly Glu Pro Leu Pro         
        275                 280                 285                     

ttg cct aag ata gaa aac cct agc gaa gag att gtt gcc aag tat cac      912
Leu Pro Lys Ile Glu Asn Pro Ser Glu Glu Ile Val Ala Lys Tyr His         
    290                 295                 300                         

acc ctt tac atc gat gct ctc aga aag cta ttc gac cag cac aag act      960
Thr Leu Tyr Ile Asp Ala Leu Arg Lys Leu Phe Asp Gln His Lys Thr         
305                 310                 315                 320         

aag ttc gga atc tct gag aca caa gag ctg gtt atc gtc taa             1002
Lys Phe Gly Ile Ser Glu Thr Gln Glu Leu Val Ile Val                     
                325                 330                                 


<210>  12
<211>  333
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  12

Met Phe Trp Pro Thr Lys Lys Asp Leu Lys Thr Ala Met Glu Val Phe 
1               5                   10                  15      


Ala Leu Phe Gln Trp Ala Leu Ser Ala Leu Val Ile Val Thr Thr Val 
            20                  25                  30          


Ile Ile Val Asn Leu Tyr Leu Val Val Phe Thr Ser Tyr Trp Pro Val 
        35                  40                  45              


Thr Val Leu Met Leu Thr Trp Leu Ala Phe Asp Trp Lys Thr Pro Glu 
    50                  55                  60                  


Arg Gly Gly Arg Arg Phe Thr Cys Val Arg Lys Trp Arg Leu Trp Lys 
65                  70                  75                  80  


His Tyr Ser Asp Tyr Phe Pro Leu Lys Met Val Lys Thr Lys Asp Ile 
                85                  90                  95      


Ser Pro Asp Arg Asn Tyr Ile Leu Val Cys His Pro His Gly Leu Met 
            100                 105                 110         


Ala His Ser Cys Phe Gly His Phe Ala Thr Asp Thr Thr Gly Phe Ser 
        115                 120                 125             


Lys Thr Phe Pro Gly Ile Thr Pro Tyr Met Leu Thr Leu Gly Ala Phe 
    130                 135                 140                 


Phe Trp Val Pro Phe Leu Arg Asp Tyr Val Met Ser Thr Gly Ser Cys 
145                 150                 155                 160 


Ser Val Ser Arg Ser Ser Met Asp Phe Leu Leu Thr Gln Lys Gly Thr 
                165                 170                 175     


Gly Asn Met Leu Val Val Val Val Gly Gly Leu Ala Glu Cys Arg Tyr 
            180                 185                 190         


Ser Thr Pro Gly Ser Thr Thr Leu Phe Leu Lys Lys Arg Gln Gly Phe 
        195                 200                 205             


Val Arg Thr Ala Leu Lys His Gly Val Ser Leu Ile Pro Ala Tyr Ala 
    210                 215                 220                 


Phe Gly Glu Thr Asp Leu Tyr Asp Gln His Ile Phe Thr Pro Gly Gly 
225                 230                 235                 240 


Phe Val Asn Arg Phe Gln Lys Trp Phe Gln Lys Met Val His Ile Tyr 
                245                 250                 255     


Pro Cys Ala Phe Tyr Gly Arg Gly Leu Thr Lys Asn Ser Trp Gly Leu 
            260                 265                 270         


Leu Pro Tyr Ser Gln Pro Val Thr Thr Val Val Gly Glu Pro Leu Pro 
        275                 280                 285             


Leu Pro Lys Ile Glu Asn Pro Ser Glu Glu Ile Val Ala Lys Tyr His 
    290                 295                 300                 


Thr Leu Tyr Ile Asp Ala Leu Arg Lys Leu Phe Asp Gln His Lys Thr 
305                 310                 315                 320 


Lys Phe Gly Ile Ser Glu Thr Gln Glu Leu Val Ile Val 
                325                 330             


<210>  13
<211>  1731
<212>  DNA
<213>  Artificial

<220>
<223>  Wax ester synthase from mouse tagged with YFP


<220>
<221>  CDS
<222>  (7)..(1725)

<400>  13
actagt atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg ccc        48
       Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro          
       1               5                   10                           

atc ctg gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc gtg       96
Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val         
15                  20                  25                  30          

tcc ggc gag ggc gag ggc gat gcc acc tac ggc aag ctg acc ctg aag      144
Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys         
                35                  40                  45              

ttc atc tgc acc acc ggc aag ctg ccc gtg ccc tgg ccc acc ctc gtg      192
Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val         
            50                  55                  60                  

acc acc ttc ggc tac ggc ctg cag tgc ttc gcc cgc tac ccc gac cac      240
Thr Thr Phe Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His         
        65                  70                  75                      

atg aag cag cac gac ttc ttc aag tcc gcc atg ccc gaa ggc tac gtc      288
Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val         
    80                  85                  90                          

cag gag cgc acc atc ttc ttc aag gac gac ggc aac tac aag acc cgc      336
Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg         
95                  100                 105                 110         

gcc gag gtg aag ttc gag ggc gac acc ctg gtg aac cgc atc gag ctg      384
Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu         
                115                 120                 125             

aag ggc atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag ctg      432
Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu         
            130                 135                 140                 

gag tac aac tac aac agc cac aac gtc tat atc atg gcc gac aag cag      480
Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln         
        145                 150                 155                     

aag aac ggc atc aag gtg aac ttc aag atc cgc cac aac atc gag gac      528
Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp         
    160                 165                 170                         

ggc agc gtg cag ctc gcc gac cac tac cag cag aac acc ccc atc ggc      576
Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly         
175                 180                 185                 190         

gac ggc ccc gtg ctg ctg ccc gac aac cac tac ctg agc tac cag tcc      624
Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser         
                195                 200                 205             

gcc ctg agc aaa gac ccc aac gag aag cgc gat cac atg gtc ctg ctg      672
Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu         
            210                 215                 220                 

gag ttc gtg acc gcc gcc ggg atc act ctc ggc atg gac gag ctg tac      720
Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr         
        225                 230                 235                     

aag atg ttc tgg cct act aag aag gac ctt aag act gca atg gag gtt      768
Lys Met Phe Trp Pro Thr Lys Lys Asp Leu Lys Thr Ala Met Glu Val         
    240                 245                 250                         

ttc gct ctt ttc caa tgg gct ttg tct gca ctc gtt atc gtt act acc      816
Phe Ala Leu Phe Gln Trp Ala Leu Ser Ala Leu Val Ile Val Thr Thr         
255                 260                 265                 270         

gtc atc atc gtg aac ctg tac ctt gtt gtg ttc act tca tac tgg cca      864
Val Ile Ile Val Asn Leu Tyr Leu Val Val Phe Thr Ser Tyr Trp Pro         
                275                 280                 285             

gtt acc gtt ttg atg cta aca tgg ctg gca ttc gat tgg aaa aca cct      912
Val Thr Val Leu Met Leu Thr Trp Leu Ala Phe Asp Trp Lys Thr Pro         
            290                 295                 300                 

gaa aga gga ggt aga agg ttc aca tgt gtg aga aag tgg cgt ctt tgg      960
Glu Arg Gly Gly Arg Arg Phe Thr Cys Val Arg Lys Trp Arg Leu Trp         
        305                 310                 315                     

aaa cac tac tct gac tac ttc cct ctt aag atg gtg aag acg aag gat     1008
Lys His Tyr Ser Asp Tyr Phe Pro Leu Lys Met Val Lys Thr Lys Asp         
    320                 325                 330                         

atc tcc cct gat aga aac tac ata ctt gtc tgt cac cca cat ggt ctt     1056
Ile Ser Pro Asp Arg Asn Tyr Ile Leu Val Cys His Pro His Gly Leu         
335                 340                 345                 350         

atg gca cat agt tgt ttc gga cat ttc gct acc gat act aca gga ttc     1104
Met Ala His Ser Cys Phe Gly His Phe Ala Thr Asp Thr Thr Gly Phe         
                355                 360                 365             

agt aag acc ttc cct ggt atc aca cct tac atg ctt act ctt gga gct     1152
Ser Lys Thr Phe Pro Gly Ile Thr Pro Tyr Met Leu Thr Leu Gly Ala         
            370                 375                 380                 

ttc ttc tgg gtt cct ttc ttg aga gat tac gtc atg tca acc gga agt     1200
Phe Phe Trp Val Pro Phe Leu Arg Asp Tyr Val Met Ser Thr Gly Ser         
        385                 390                 395                     

tgt tct gtg tcc aga agc tct atg gac ttc ttg ctt acg caa aag ggt     1248
Cys Ser Val Ser Arg Ser Ser Met Asp Phe Leu Leu Thr Gln Lys Gly         
    400                 405                 410                         

aca gga aac atg ctt gtt gtc gtt gtt ggt gga ctt gct gaa tgt aga     1296
Thr Gly Asn Met Leu Val Val Val Val Gly Gly Leu Ala Glu Cys Arg         
415                 420                 425                 430         

tac tct aca cca ggc tca aca acc ctt ttc cta aag aag agg cag gga     1344
Tyr Ser Thr Pro Gly Ser Thr Thr Leu Phe Leu Lys Lys Arg Gln Gly         
                435                 440                 445             

ttt gtt cgt aca gca cta aag cat ggg gtt tct ttg atc cct gct tat     1392
Phe Val Arg Thr Ala Leu Lys His Gly Val Ser Leu Ile Pro Ala Tyr         
            450                 455                 460                 

gca ttt gga gag act gac ctc tac gat caa cat atc ttc act cct gga     1440
Ala Phe Gly Glu Thr Asp Leu Tyr Asp Gln His Ile Phe Thr Pro Gly         
        465                 470                 475                     

gga ttc gtc aac aga ttc caa aag tgg ttc cag aag atg gtt cac atc     1488
Gly Phe Val Asn Arg Phe Gln Lys Trp Phe Gln Lys Met Val His Ile         
    480                 485                 490                         

tac cca tgt gct ttc tat gga aga ggt ctg acc aag aat tca tgg ggt     1536
Tyr Pro Cys Ala Phe Tyr Gly Arg Gly Leu Thr Lys Asn Ser Trp Gly         
495                 500                 505                 510         

ctt ttg cct tac tca caa cca gtt act aca gtt gtt gga gag cca tta     1584
Leu Leu Pro Tyr Ser Gln Pro Val Thr Thr Val Val Gly Glu Pro Leu         
                515                 520                 525             

ccg ttg cct aag ata gaa aac cct agc gaa gag att gtt gcc aag tat     1632
Pro Leu Pro Lys Ile Glu Asn Pro Ser Glu Glu Ile Val Ala Lys Tyr         
            530                 535                 540                 

cac acc ctt tac atc gat gct ctc aga aag cta ttc gac cag cac aag     1680
His Thr Leu Tyr Ile Asp Ala Leu Arg Lys Leu Phe Asp Gln His Lys         
        545                 550                 555                     

act aag ttc gga atc tct gag aca caa gag ctg gtt atc gtc taa         1725
Thr Lys Phe Gly Ile Ser Glu Thr Gln Glu Leu Val Ile Val                 
    560                 565                 570                         

gagctc                                                              1731


<210>  14
<211>  572
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  14

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Phe Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Met 
225                 230                 235                 240 


Phe Trp Pro Thr Lys Lys Asp Leu Lys Thr Ala Met Glu Val Phe Ala 
                245                 250                 255     


Leu Phe Gln Trp Ala Leu Ser Ala Leu Val Ile Val Thr Thr Val Ile 
            260                 265                 270         


Ile Val Asn Leu Tyr Leu Val Val Phe Thr Ser Tyr Trp Pro Val Thr 
        275                 280                 285             


Val Leu Met Leu Thr Trp Leu Ala Phe Asp Trp Lys Thr Pro Glu Arg 
    290                 295                 300                 


Gly Gly Arg Arg Phe Thr Cys Val Arg Lys Trp Arg Leu Trp Lys His 
305                 310                 315                 320 


Tyr Ser Asp Tyr Phe Pro Leu Lys Met Val Lys Thr Lys Asp Ile Ser 
                325                 330                 335     


Pro Asp Arg Asn Tyr Ile Leu Val Cys His Pro His Gly Leu Met Ala 
            340                 345                 350         


His Ser Cys Phe Gly His Phe Ala Thr Asp Thr Thr Gly Phe Ser Lys 
        355                 360                 365             


Thr Phe Pro Gly Ile Thr Pro Tyr Met Leu Thr Leu Gly Ala Phe Phe 
    370                 375                 380                 


Trp Val Pro Phe Leu Arg Asp Tyr Val Met Ser Thr Gly Ser Cys Ser 
385                 390                 395                 400 


Val Ser Arg Ser Ser Met Asp Phe Leu Leu Thr Gln Lys Gly Thr Gly 
                405                 410                 415     


Asn Met Leu Val Val Val Val Gly Gly Leu Ala Glu Cys Arg Tyr Ser 
            420                 425                 430         


Thr Pro Gly Ser Thr Thr Leu Phe Leu Lys Lys Arg Gln Gly Phe Val 
        435                 440                 445             


Arg Thr Ala Leu Lys His Gly Val Ser Leu Ile Pro Ala Tyr Ala Phe 
    450                 455                 460                 


Gly Glu Thr Asp Leu Tyr Asp Gln His Ile Phe Thr Pro Gly Gly Phe 
465                 470                 475                 480 


Val Asn Arg Phe Gln Lys Trp Phe Gln Lys Met Val His Ile Tyr Pro 
                485                 490                 495     


Cys Ala Phe Tyr Gly Arg Gly Leu Thr Lys Asn Ser Trp Gly Leu Leu 
            500                 505                 510         


Pro Tyr Ser Gln Pro Val Thr Thr Val Val Gly Glu Pro Leu Pro Leu 
        515                 520                 525             


Pro Lys Ile Glu Asn Pro Ser Glu Glu Ile Val Ala Lys Tyr His Thr 
    530                 535                 540                 


Leu Tyr Ile Asp Ala Leu Arg Lys Leu Phe Asp Gln His Lys Thr Lys 
545                 550                 555                 560 


Phe Gly Ile Ser Glu Thr Gln Glu Leu Val Ile Val 
                565                 570         


<210>  15
<211>  2154
<212>  DNA
<213>  Artificial

<220>
<223>  Wax ester synthase from mouse tagged with YFP fused to oleosin 3 
       At


<220>
<221>  CDS
<222>  (7)..(2148)

<400>  15
actagt atg gcg gac caa aca aga acc cat cac gag atg ata agc cga        48
       Met Ala Asp Gln Thr Arg Thr His His Glu Met Ile Ser Arg          
       1               5                   10                           

gac agt acc caa gag gcc cat ccg aag gcc agg cag atg gtg aag gca       96
Asp Ser Thr Gln Glu Ala His Pro Lys Ala Arg Gln Met Val Lys Ala         
15                  20                  25                  30          

gca acc gct gtc aca gcc ggt gga tcc cta ctt gtc ctc tcc ggc tta      144
Ala Thr Ala Val Thr Ala Gly Gly Ser Leu Leu Val Leu Ser Gly Leu         
                35                  40                  45              

aca ctc gct gga aca gtc atc gca ctc acg gtg gct act cct ctc ctc      192
Thr Leu Ala Gly Thr Val Ile Ala Leu Thr Val Ala Thr Pro Leu Leu         
            50                  55                  60                  

gtc atc ttc agc ccc gtc ttg gtt cca gca gtg gta acc gtt gct ctc      240
Val Ile Phe Ser Pro Val Leu Val Pro Ala Val Val Thr Val Ala Leu         
        65                  70                  75                      

atc att acc gga ttc ctt gca tcc ggt ggc ttt gga ata gcc gcc att      288
Ile Ile Thr Gly Phe Leu Ala Ser Gly Gly Phe Gly Ile Ala Ala Ile         
    80                  85                  90                          

acc gcc ttc tct tgg ctc tac agg cac atg acg gga tct gga tcg gat      336
Thr Ala Phe Ser Trp Leu Tyr Arg His Met Thr Gly Ser Gly Ser Asp         
95                  100                 105                 110         

aag ata gag aat gct cgg atg aag gtt gga agc aga gtg cag gat act      384
Lys Ile Glu Asn Ala Arg Met Lys Val Gly Ser Arg Val Gln Asp Thr         
                115                 120                 125             

aag tat ggg cag cac aac att gga gtc caa cac caa caa gtt tct atg      432
Lys Tyr Gly Gln His Asn Ile Gly Val Gln His Gln Gln Val Ser Met         
            130                 135                 140                 

gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg ccc atc ctg gtc      480
Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val         
        145                 150                 155                     

gag ctg gac ggc gac gta aac ggc cac aag ttc agc gtg tcc ggc gag      528
Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu         
    160                 165                 170                         

ggc gag ggc gat gcc acc tac ggc aag ctg acc ctg aag ttc atc tgc      576
Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys         
175                 180                 185                 190         

acc acc ggc aag ctg ccc gtg ccc tgg ccc acc ctc gtg acc acc ttc      624
Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe         
                195                 200                 205             

ggc tac ggc ctg cag tgc ttc gcc cgc tac ccc gac cac atg aag cag      672
Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln         
            210                 215                 220                 

cac gac ttc ttc aag tcc gcc atg ccc gaa ggc tac gtc cag gag cgc      720
His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg         
        225                 230                 235                     

acc atc ttc ttc aag gac gac ggc aac tac aag acc cgc gcc gag gtg      768
Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val         
    240                 245                 250                         

aag ttc gag ggc gac acc ctg gtg aac cgc atc gag ctg aag ggc atc      816
Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile         
255                 260                 265                 270         

gac ttc aag gag gac ggc aac atc ctg ggg cac aag ctg gag tac aac      864
Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn         
                275                 280                 285             

tac aac agc cac aac gtc tat atc atg gcc gac aag cag aag aac ggc      912
Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly         
            290                 295                 300                 

atc aag gtg aac ttc aag atc cgc cac aac atc gag gac ggc agc gtg      960
Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val         
        305                 310                 315                     

cag ctc gcc gac cac tac cag cag aac acc ccc atc ggc gac ggc ccc     1008
Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro         
    320                 325                 330                         

gtg ctg ctg ccc gac aac cac tac ctg agc tac cag tcc gcc ctg agc     1056
Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu Ser         
335                 340                 345                 350         

aaa gac ccc aac gag aag cgc gat cac atg gtc ctg ctg gag ttc gtg     1104
Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val         
                355                 360                 365             

acc gcc gcc ggg atc act ctc ggc atg gac gag ctg tac aag atg ttc     1152
Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Met Phe         
            370                 375                 380                 

tgg cct act aag aag gac ctt aag act gca atg gag gtt ttc gct ctt     1200
Trp Pro Thr Lys Lys Asp Leu Lys Thr Ala Met Glu Val Phe Ala Leu         
        385                 390                 395                     

ttc caa tgg gct ttg tct gca ctc gtt atc gtt act acc gtc atc atc     1248
Phe Gln Trp Ala Leu Ser Ala Leu Val Ile Val Thr Thr Val Ile Ile         
    400                 405                 410                         

gtg aac ctg tac ctt gtt gtg ttc act tca tac tgg cca gtt acc gtt     1296
Val Asn Leu Tyr Leu Val Val Phe Thr Ser Tyr Trp Pro Val Thr Val         
415                 420                 425                 430         

ttg atg cta aca tgg ctg gca ttc gat tgg aaa aca cct gaa aga gga     1344
Leu Met Leu Thr Trp Leu Ala Phe Asp Trp Lys Thr Pro Glu Arg Gly         
                435                 440                 445             

ggt aga agg ttc aca tgt gtg aga aag tgg cgt ctt tgg aaa cac tac     1392
Gly Arg Arg Phe Thr Cys Val Arg Lys Trp Arg Leu Trp Lys His Tyr         
            450                 455                 460                 

tct gac tac ttc cct ctt aag atg gtg aag acg aag gat atc tcc cct     1440
Ser Asp Tyr Phe Pro Leu Lys Met Val Lys Thr Lys Asp Ile Ser Pro         
        465                 470                 475                     

gat aga aac tac ata ctt gtc tgt cac cca cat ggt ctt atg gca cat     1488
Asp Arg Asn Tyr Ile Leu Val Cys His Pro His Gly Leu Met Ala His         
    480                 485                 490                         

agt tgt ttc gga cat ttc gct acc gat act aca gga ttc agt aag acc     1536
Ser Cys Phe Gly His Phe Ala Thr Asp Thr Thr Gly Phe Ser Lys Thr         
495                 500                 505                 510         

ttc cct ggt atc aca cct tac atg ctt act ctt gga gct ttc ttc tgg     1584
Phe Pro Gly Ile Thr Pro Tyr Met Leu Thr Leu Gly Ala Phe Phe Trp         
                515                 520                 525             

gtt cct ttc ttg aga gat tac gtc atg tca acc gga agt tgt tct gtg     1632
Val Pro Phe Leu Arg Asp Tyr Val Met Ser Thr Gly Ser Cys Ser Val         
            530                 535                 540                 

tcc aga agc tct atg gac ttc ttg ctt acg caa aag ggt aca gga aac     1680
Ser Arg Ser Ser Met Asp Phe Leu Leu Thr Gln Lys Gly Thr Gly Asn         
        545                 550                 555                     

atg ctt gtt gtc gtt gtt ggt gga ctt gct gaa tgt aga tac tct aca     1728
Met Leu Val Val Val Val Gly Gly Leu Ala Glu Cys Arg Tyr Ser Thr         
    560                 565                 570                         

cca ggc tca aca acc ctt ttc cta aag aag agg cag gga ttt gtt cgt     1776
Pro Gly Ser Thr Thr Leu Phe Leu Lys Lys Arg Gln Gly Phe Val Arg         
575                 580                 585                 590         

aca gca cta aag cat ggg gtt tct ttg atc cct gct tat gca ttt gga     1824
Thr Ala Leu Lys His Gly Val Ser Leu Ile Pro Ala Tyr Ala Phe Gly         
                595                 600                 605             

gag act gac ctc tac gat caa cat atc ttc act cct gga gga ttc gtc     1872
Glu Thr Asp Leu Tyr Asp Gln His Ile Phe Thr Pro Gly Gly Phe Val         
            610                 615                 620                 

aac aga ttc caa aag tgg ttc cag aag atg gtt cac atc tac cca tgt     1920
Asn Arg Phe Gln Lys Trp Phe Gln Lys Met Val His Ile Tyr Pro Cys         
        625                 630                 635                     

gct ttc tat gga aga ggt ctg acc aag aat tca tgg ggt ctt ttg cct     1968
Ala Phe Tyr Gly Arg Gly Leu Thr Lys Asn Ser Trp Gly Leu Leu Pro         
    640                 645                 650                         

tac tca caa cca gtt act aca gtt gtt gga gag cca tta ccg ttg cct     2016
Tyr Ser Gln Pro Val Thr Thr Val Val Gly Glu Pro Leu Pro Leu Pro         
655                 660                 665                 670         

aag ata gaa aac cct agc gaa gag att gtt gcc aag tat cac acc ctt     2064
Lys Ile Glu Asn Pro Ser Glu Glu Ile Val Ala Lys Tyr His Thr Leu         
                675                 680                 685             

tac atc gat gct ctc aga aag cta ttc gac cag cac aag act aag ttc     2112
Tyr Ile Asp Ala Leu Arg Lys Leu Phe Asp Gln His Lys Thr Lys Phe         
            690                 695                 700                 

gga atc tct gag aca caa gag ctg gtt atc gtc taa gagctc              2154
Gly Ile Ser Glu Thr Gln Glu Leu Val Ile Val                             
        705                 710                                         


<210>  16
<211>  713
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  16

Met Ala Asp Gln Thr Arg Thr His His Glu Met Ile Ser Arg Asp Ser 
1               5                   10                  15      


Thr Gln Glu Ala His Pro Lys Ala Arg Gln Met Val Lys Ala Ala Thr 
            20                  25                  30          


Ala Val Thr Ala Gly Gly Ser Leu Leu Val Leu Ser Gly Leu Thr Leu 
        35                  40                  45              


Ala Gly Thr Val Ile Ala Leu Thr Val Ala Thr Pro Leu Leu Val Ile 
    50                  55                  60                  


Phe Ser Pro Val Leu Val Pro Ala Val Val Thr Val Ala Leu Ile Ile 
65                  70                  75                  80  


Thr Gly Phe Leu Ala Ser Gly Gly Phe Gly Ile Ala Ala Ile Thr Ala 
                85                  90                  95      


Phe Ser Trp Leu Tyr Arg His Met Thr Gly Ser Gly Ser Asp Lys Ile 
            100                 105                 110         


Glu Asn Ala Arg Met Lys Val Gly Ser Arg Val Gln Asp Thr Lys Tyr 
        115                 120                 125             


Gly Gln His Asn Ile Gly Val Gln His Gln Gln Val Ser Met Val Ser 
    130                 135                 140                 


Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu 
145                 150                 155                 160 


Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu 
                165                 170                 175     


Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr 
            180                 185                 190         


Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe Gly Tyr 
        195                 200                 205             


Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln His Asp 
    210                 215                 220                 


Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile 
225                 230                 235                 240 


Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe 
                245                 250                 255     


Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe 
            260                 265                 270         


Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn 
        275                 280                 285             


Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys 
    290                 295                 300                 


Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu 
305                 310                 315                 320 


Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 
                325                 330                 335     


Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu Ser Lys Asp 
            340                 345                 350         


Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala 
        355                 360                 365             


Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Met Phe Trp Pro 
    370                 375                 380                 


Thr Lys Lys Asp Leu Lys Thr Ala Met Glu Val Phe Ala Leu Phe Gln 
385                 390                 395                 400 


Trp Ala Leu Ser Ala Leu Val Ile Val Thr Thr Val Ile Ile Val Asn 
                405                 410                 415     


Leu Tyr Leu Val Val Phe Thr Ser Tyr Trp Pro Val Thr Val Leu Met 
            420                 425                 430         


Leu Thr Trp Leu Ala Phe Asp Trp Lys Thr Pro Glu Arg Gly Gly Arg 
        435                 440                 445             


Arg Phe Thr Cys Val Arg Lys Trp Arg Leu Trp Lys His Tyr Ser Asp 
    450                 455                 460                 


Tyr Phe Pro Leu Lys Met Val Lys Thr Lys Asp Ile Ser Pro Asp Arg 
465                 470                 475                 480 


Asn Tyr Ile Leu Val Cys His Pro His Gly Leu Met Ala His Ser Cys 
                485                 490                 495     


Phe Gly His Phe Ala Thr Asp Thr Thr Gly Phe Ser Lys Thr Phe Pro 
            500                 505                 510         


Gly Ile Thr Pro Tyr Met Leu Thr Leu Gly Ala Phe Phe Trp Val Pro 
        515                 520                 525             


Phe Leu Arg Asp Tyr Val Met Ser Thr Gly Ser Cys Ser Val Ser Arg 
    530                 535                 540                 


Ser Ser Met Asp Phe Leu Leu Thr Gln Lys Gly Thr Gly Asn Met Leu 
545                 550                 555                 560 


Val Val Val Val Gly Gly Leu Ala Glu Cys Arg Tyr Ser Thr Pro Gly 
                565                 570                 575     


Ser Thr Thr Leu Phe Leu Lys Lys Arg Gln Gly Phe Val Arg Thr Ala 
            580                 585                 590         


Leu Lys His Gly Val Ser Leu Ile Pro Ala Tyr Ala Phe Gly Glu Thr 
        595                 600                 605             


Asp Leu Tyr Asp Gln His Ile Phe Thr Pro Gly Gly Phe Val Asn Arg 
    610                 615                 620                 


Phe Gln Lys Trp Phe Gln Lys Met Val His Ile Tyr Pro Cys Ala Phe 
625                 630                 635                 640 


Tyr Gly Arg Gly Leu Thr Lys Asn Ser Trp Gly Leu Leu Pro Tyr Ser 
                645                 650                 655     


Gln Pro Val Thr Thr Val Val Gly Glu Pro Leu Pro Leu Pro Lys Ile 
            660                 665                 670         


Glu Asn Pro Ser Glu Glu Ile Val Ala Lys Tyr His Thr Leu Tyr Ile 
        675                 680                 685             


Asp Ala Leu Arg Lys Leu Phe Asp Gln His Lys Thr Lys Phe Gly Ile 
    690                 695                 700                 


Ser Glu Thr Gln Glu Leu Val Ile Val 
705                 710             

