                         SEQUENCE LISTING

<110>  Armstrong, Charles
 
<120>  Thermophilic Mannanohydrolase and Fracturing Fluids Containing 
       the Same

<130>  020569-20900

<140>  12/579,771
<141>  2009-10-15

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  1053
<212>  DNA
<213>  Artificial Sequence


<220>
<221>  CDS
<222>  (7)..(1044)
<223>  Caldocellum saccharolyticum

<400>  1
ggatcc atg cgc ctg aaa acc aaa atc cgc aaa aag tgg ctg tca gtg          48
       Met Arg Leu Lys Thr Lys Ile Arg Lys Lys Trp Leu Ser Val            
       1               5                   10                             

ctg tgc act gta gtc ttt ctg ctg aat att ctg ttt att gcg aac gtt         96
Leu Cys Thr Val Val Phe Leu Leu Asn Ile Leu Phe Ile Ala Asn Val           
15                  20                  25                  30            

acc atc ctg cca aaa gta ggc gcg gct acc tcc aac gat ggt gtg gtt        144
Thr Ile Leu Pro Lys Val Gly Ala Ala Thr Ser Asn Asp Gly Val Val           
                35                  40                  45                

aaa att gat acc tcg acc ctg att ggt acc aat cat gct cat tgc tgg        192
Lys Ile Asp Thr Ser Thr Leu Ile Gly Thr Asn His Ala His Cys Trp           
            50                  55                  60                    

tat cgc gat cgt ctg gat acc gcg ctg cgc gga att cgt agt tgg ggt        240
Tyr Arg Asp Arg Leu Asp Thr Ala Leu Arg Gly Ile Arg Ser Trp Gly           
        65                  70                  75                        

atg aac tcg gta cgc gtc gtt ctg tct aat ggc tat cgc tgg aca aaa        288
Met Asn Ser Val Arg Val Val Leu Ser Asn Gly Tyr Arg Trp Thr Lys           
    80                  85                  90                            

att ccg gcc agc gaa gtt gcc aac att att tcg ctg tcc cgc tcc ctg        336
Ile Pro Ala Ser Glu Val Ala Asn Ile Ile Ser Leu Ser Arg Ser Leu           
95                  100                 105                 110           

ggc ttc aaa gcc att att ctg gag gtg cat gat acc acc ggt tac ggt        384
Gly Phe Lys Ala Ile Ile Leu Glu Val His Asp Thr Thr Gly Tyr Gly           
                115                 120                 125               

gaa gat ggt gcg gcg tgc tcc ctg gca cag gca gtt gaa tat tgg aaa        432
Glu Asp Gly Ala Ala Cys Ser Leu Ala Gln Ala Val Glu Tyr Trp Lys           
            130                 135                 140                   

gag atc aaa agc gtg ctg gat ggc aat gaa gat ttt gtc atc atc aat        480
Glu Ile Lys Ser Val Leu Asp Gly Asn Glu Asp Phe Val Ile Ile Asn           
        145                 150                 155                       

att ggt aat gaa ccg tat ggt aat aac aac tat cag aac tgg gta aat        528
Ile Gly Asn Glu Pro Tyr Gly Asn Asn Asn Tyr Gln Asn Trp Val Asn           
    160                 165                 170                           

gat act aag aat gca att aaa gcg ctg cgc gat gcc ggc ttt aag cat        576
Asp Thr Lys Asn Ala Ile Lys Ala Leu Arg Asp Ala Gly Phe Lys His           
175                 180                 185                 190           

acc atc atg gta gat gcg ccg aac tgg ggc cag gat tgg tcg aat acc        624
Thr Ile Met Val Asp Ala Pro Asn Trp Gly Gln Asp Trp Ser Asn Thr           
                195                 200                 205               

atg cgc gac aat gct cag tct att atg gaa gcc gat cca ctg cgt aat        672
Met Arg Asp Asn Ala Gln Ser Ile Met Glu Ala Asp Pro Leu Arg Asn           
            210                 215                 220                   

ctg gta ttt agc att cac atg tac ggt gtc tat aat act gcg agc aaa        720
Leu Val Phe Ser Ile His Met Tyr Gly Val Tyr Asn Thr Ala Ser Lys           
        225                 230                 235                       

gtg gaa gaa tat atc aaa agt ttt gtg gat aaa ggt ctg ccg ctg gtt        768
Val Glu Glu Tyr Ile Lys Ser Phe Val Asp Lys Gly Leu Pro Leu Val           
    240                 245                 250                           

atc ggc gaa ttc ggt cac cag cac act gat ggt gac cct gat gaa gag        816
Ile Gly Glu Phe Gly His Gln His Thr Asp Gly Asp Pro Asp Glu Glu           
255                 260                 265                 270           

gcg atc gtt cgc tat gcc aaa cag tat aaa att ggc ctg ttt agt tgg        864
Ala Ile Val Arg Tyr Ala Lys Gln Tyr Lys Ile Gly Leu Phe Ser Trp           
                275                 280                 285               

agt tgg tgt ggg aac agc agt tac gtc ggt tac ctg gat atg gtg aat        912
Ser Trp Cys Gly Asn Ser Ser Tyr Val Gly Tyr Leu Asp Met Val Asn           
            290                 295                 300                   

aac tgg gac ccg aac aac ccg acc cca tgg ggg cag tgg tat aaa aca        960
Asn Trp Asp Pro Asn Asn Pro Thr Pro Trp Gly Gln Trp Tyr Lys Thr           
        305                 310                 315                       

aat gcg atc ggc acg tca agc acg ccg acc ccg aca tcg act gtc acc       1008
Asn Ala Ile Gly Thr Ser Ser Thr Pro Thr Pro Thr Ser Thr Val Thr           
    320                 325                 330                           

cca acg cca ccg ccg cgc cag cac cag cat cgc caa taaaagctt             1053
Pro Thr Pro Pro Pro Arg Gln His Gln His Arg Gln                           
335                 340                 345                               


<210>  2
<211>  346
<212>  PRT
<213>  Artificial Sequence


<220>  
<223>  Calocellum saccharolyticum

<400>  2

Met Arg Leu Lys Thr Lys Ile Arg Lys Lys Trp Leu Ser Val Leu Cys 
1               5                   10                  15      


Thr Val Val Phe Leu Leu Asn Ile Leu Phe Ile Ala Asn Val Thr Ile 
            20                  25                  30          


Leu Pro Lys Val Gly Ala Ala Thr Ser Asn Asp Gly Val Val Lys Ile 
        35                  40                  45              


Asp Thr Ser Thr Leu Ile Gly Thr Asn His Ala His Cys Trp Tyr Arg 
    50                  55                  60                  


Asp Arg Leu Asp Thr Ala Leu Arg Gly Ile Arg Ser Trp Gly Met Asn 
65                  70                  75                  80  


Ser Val Arg Val Val Leu Ser Asn Gly Tyr Arg Trp Thr Lys Ile Pro 
                85                  90                  95      


Ala Ser Glu Val Ala Asn Ile Ile Ser Leu Ser Arg Ser Leu Gly Phe 
            100                 105                 110         


Lys Ala Ile Ile Leu Glu Val His Asp Thr Thr Gly Tyr Gly Glu Asp 
        115                 120                 125             


Gly Ala Ala Cys Ser Leu Ala Gln Ala Val Glu Tyr Trp Lys Glu Ile 
    130                 135                 140                 


Lys Ser Val Leu Asp Gly Asn Glu Asp Phe Val Ile Ile Asn Ile Gly 
145                 150                 155                 160 


Asn Glu Pro Tyr Gly Asn Asn Asn Tyr Gln Asn Trp Val Asn Asp Thr 
                165                 170                 175     


Lys Asn Ala Ile Lys Ala Leu Arg Asp Ala Gly Phe Lys His Thr Ile 
            180                 185                 190         


Met Val Asp Ala Pro Asn Trp Gly Gln Asp Trp Ser Asn Thr Met Arg 
        195                 200                 205             


Asp Asn Ala Gln Ser Ile Met Glu Ala Asp Pro Leu Arg Asn Leu Val 
    210                 215                 220                 


Phe Ser Ile His Met Tyr Gly Val Tyr Asn Thr Ala Ser Lys Val Glu 
225                 230                 235                 240 


Glu Tyr Ile Lys Ser Phe Val Asp Lys Gly Leu Pro Leu Val Ile Gly 
                245                 250                 255     


Glu Phe Gly His Gln His Thr Asp Gly Asp Pro Asp Glu Glu Ala Ile 
            260                 265                 270         


Val Arg Tyr Ala Lys Gln Tyr Lys Ile Gly Leu Phe Ser Trp Ser Trp 
        275                 280                 285             


Cys Gly Asn Ser Ser Tyr Val Gly Tyr Leu Asp Met Val Asn Asn Trp 
    290                 295                 300                 


Asp Pro Asn Asn Pro Thr Pro Trp Gly Gln Trp Tyr Lys Thr Asn Ala 
305                 310                 315                 320 


Ile Gly Thr Ser Ser Thr Pro Thr Pro Thr Ser Thr Val Thr Pro Thr 
                325                 330                 335     


Pro Pro Pro Arg Gln His Gln His Arg Gln 
            340                 345     


