                         SEQUENCE LISTING

<110>  ZHANG, Weiping
 
<120>  ENTEROTOXIGENIC E. COLI FUSION PROTEIN VACCINES

<130>  16207-0025

<150>  US 61/250,427
<151>  2009-10-09

<160>  61    

<170>  PatentIn version 3.5

<210>  1
<211>  19
<212>  PRT
<213>  E. coli


<220>
<221>  misc_feature
<223>  human E. coli strain STa protein

<400>  1

Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr 
1               5                   10                  15      


Gly Cys Tyr 
            


<210>  2
<211>  63
<212>  DNA
<213>  E. coli


<220>
<221>  misc_feature
<223>  native human E. coli strain STa nucleotide sequence

<400>  2
atgaatagta gcaattactg ctgtgaattg tgttgtaatc ctgcttgtac cgggtgctat       60

taa                                                                     63


<210>  3
<211>  19
<212>  PRT
<213>  E. coli


<220>
<221>  misc_feature
<223>  native porcine E. coli strain STa protein

<400>  3

Asn Asn Thr Phe Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Ala 
1               5                   10                  15      


Gly Cys Tyr 
            


<210>  4
<211>  63
<212>  DNA
<213>  E. coli


<220>
<221>  misc_feature
<223>  native porcine E. coli strain STa nucleotide sequence

<400>  4
atgaacaaca cattttactg ctgtgaactt tgttgtaatc ctgcctgtgc tggatgttat       60

taa                                                                     63


<210>  5
<211>  868
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA:  Mutation of the eltAB gene 

<400>  5
atgattgaca tcatgttgca tataggttag ataaaacaag tggttatctt tccggattgt       60

cttcttgtat gatatataag ttttcctcga tgaaaaatat aactttcatt ttttttattt      120

tattagcatc gccattatat gcaaatggcg acagattata ccgtgctgac tctagacccc      180

cagatgaaat aaaacgtttc cggagtctta tgcccagagg taatgagtac ttcgatagag      240

gaactcaaat gaatattaat ctttatgatc acgcgagagg aacacaaacc ggctttgtca      300

gatatgatga cggatatgtt tccacttctc ttagtttgag aagtgctcac ttagcaggac      360

agtatatatt atcaggatat tcacttacta tatatatcgt tatagcaaat atgtttaatg      420

ttaatgatgt aattagcgta tacagccctc acccatatga acaggaggtt tctgcgttag      480

gtggaatacc atattctcag atatatggat ggtatcgtgt taattttggt gtgattgatg      540

aacgattaca tcgtaacagg gaatatagag accggtatta cagaaatctg aatatagctc      600

cggcagagga tggttacaga ttagcaggtt tcccaccgga tcaccaagct tggagagaag      660

aaccctggat tcatcatgca ccacaaggtt gtggagattc atcaagaaca atcacaggtg      720

atacttgtaa tgaggagacc cagaatctga gcacaatata tctcagggaa tatcaatcaa      780

aagttaagag gcagatattt tcagactatc agtcagaggt tgacatatat aacagaattc      840

gggatgaatt atgaataaag taaaatgt                                         868


<210>  6
<211>  382
<212>  PRT
<213>  E. coli


<220>
<221>  misc_feature
<223>  native LT protein

<400>  6

Met Lys Asn Ile Thr Phe Ile Phe Phe Ile Leu Leu Ala Ser Pro Leu 
1               5                   10                  15      


Tyr Ala Asn Gly Asp Arg Leu Tyr Arg Ala Asp Ser Arg Pro Pro Asp 
            20                  25                  30          


Glu Ile Lys Arg Ser Gly Gly Leu Met Pro Arg Gly His Asn Glu Tyr 
        35                  40                  45              


Phe Asp Arg Gly Thr Gln Met Asn Ile Asn Leu Tyr Asp His Ala Arg 
    50                  55                  60                  


Gly Thr Gln Thr Gly Phe Val Arg Tyr Asp Asp Gly Tyr Val Ser Thr 
65                  70                  75                  80  


Ser Leu Ser Leu Arg Ser Ala His Leu Ala Gly Gln Tyr Ile Leu Ser 
                85                  90                  95      


Gly Tyr Ser Thr Tyr Tyr Ile Tyr Val Ile Ala Thr Ala Pro Asn Met 
            100                 105                 110         


Phe Asn Val Asn Asp Val Leu Gly Val Tyr Ser Pro His Pro Tyr Glu 
        115                 120                 125             


Gln Glu Val Ser Ala Leu Gly Gly Ile Pro Tyr Ser Gln Ile Tyr Gly 
    130                 135                 140                 


Trp Tyr Arg Val Asn Phe Gly Val Ile Asp Glu Arg Leu His Arg Asn 
145                 150                 155                 160 


Arg Glu Tyr Arg Asp Arg Tyr Tyr Arg Asn Leu Asn Ile Ala Pro Ala 
                165                 170                 175     


Glu Asp Gly Tyr Arg Leu Ala Gly Phe Pro Pro Asp His Gln Ala Trp 
            180                 185                 190         


Arg Glu Glu Pro Trp Ile His His Ala Pro Gln Gly Cys Gly Asp Ser 
        195                 200                 205             


Ser Gly Thr Ile Thr Gly Asp Thr Cys Asn Glu Glu Thr Gln Asn Leu 
    210                 215                 220                 


Ser Thr Ile Tyr Leu Arg Glu Tyr Gln Ser Lys Val Lys Arg Gln Ile 
225                 230                 235                 240 


Phe Ser Asp Tyr Gln Ser Glu Val Asp Ile Tyr Asn Arg Ile Arg Asp 
                245                 250                 255     


Glu Leu Met Asn Lys Val Lys Cys Tyr Val Leu Phe Thr Ala Leu Leu 
            260                 265                 270         


Ser Ser Leu Tyr Ala His Gly Ala Pro Gln Thr Ile Thr Glu Leu Cys 
        275                 280                 285             


Ser Glu Tyr Arg Asn Thr Gln Ile Tyr Thr Ile Asn Asp Lys Ile Leu 
    290                 295                 300                 


Ser Tyr Thr Glu Ser Met Ala Gly Lys Arg Glu Met Val Ile Ile Thr 
305                 310                 315                 320 


Phe Lys Ser Gly Glu Thr Phe Gln Val Gly Val Pro Gly Ser Gln His 
                325                 330                 335     


Ile Asp Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu Arg 
            340                 345                 350         


Ile Thr Tyr Leu Thr Glu Thr Lys Ile Asp Lys Leu Cys Val Trp Asn 
        355                 360                 365             


Asn Lys Thr Pro Asn Ser Ile Ala Ala Ile Ser Met Lys Asn 
    370                 375                 380         


<210>  7
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide encoding a linker 

<400>  7
gggccggggc cc                                                           12


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide of an L-linker sequence 

<400>  8
cgagctcggt acccggggat c                                                 21


<210>  9
<211>  402
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic fusion protein 

<400>  9

Met Lys Asn Ile Thr Phe Ile Phe Phe Ile Leu Leu Ala Ser Pro Leu 
1               5                   10                  15      


Tyr Ala Asn Gly Asp Arg Leu Tyr Arg Ala Asp Ser Arg Pro Pro Asp 
            20                  25                  30          


Glu Ile Lys Arg Ser Gly Gly Leu Met Pro Arg Gly His Asn Glu Tyr 
        35                  40                  45              


Phe Asp Arg Gly Thr Gln Met Asn Ile Asn Leu Tyr Asp His Ala Arg 
    50                  55                  60                  


Gly Thr Gln Thr Gly Phe Val Arg Tyr Asp Asp Gly Tyr Val Ser Thr 
65                  70                  75                  80  


Ser Leu Ser Leu Arg Ser Ala His Leu Ala Gly Gln Tyr Ile Leu Ser 
                85                  90                  95      


Gly Tyr Ser Thr Tyr Tyr Ile Tyr Val Ile Ala Thr Ala Pro Asn Met 
            100                 105                 110         


Phe Asn Val Asn Asp Val Leu Gly Val Tyr Ser Pro His Pro Tyr Glu 
        115                 120                 125             


Gln Glu Val Ser Ala Leu Gly Gly Ile Pro Tyr Ser Gln Ile Tyr Gly 
    130                 135                 140                 


Trp Tyr Arg Val Asn Phe Gly Val Ile Asp Glu Arg Leu His Arg Asn 
145                 150                 155                 160 


Arg Glu Tyr Arg Asp Arg Tyr Tyr Arg Asn Leu Asn Ile Ala Pro Ala 
                165                 170                 175     


Glu Asp Gly Tyr Arg Leu Ala Gly Phe Pro Pro Asp His Gln Ala Trp 
            180                 185                 190         


Arg Glu Glu Pro Trp Ile His His Ala Pro Gln Gly Cys Gly Asp Ser 
        195                 200                 205             


Ser Gly Thr Ile Thr Gly Asp Thr Cys Asn Glu Glu Thr Gln Asn Leu 
    210                 215                 220                 


Ser Thr Ile Tyr Leu Arg Glu Tyr Gln Ser Lys Val Lys Arg Gln Ile 
225                 230                 235                 240 


Phe Ser Asp Tyr Gln Ser Glu Val Asp Ile Tyr Asn Arg Ile Arg Asp 
                245                 250                 255     


Glu Leu Met Asn Lys Val Lys Cys Tyr Val Leu Phe Thr Ala Leu Leu 
            260                 265                 270         


Ser Ser Leu Tyr Ala His Gly Ala Pro Gln Thr Ile Thr Glu Leu Cys 
        275                 280                 285             


Ser Glu Tyr Arg Asn Thr Gln Ile Tyr Thr Ile Asn Asp Lys Ile Leu 
    290                 295                 300                 


Ser Tyr Thr Glu Ser Met Ala Gly Lys Arg Glu Met Val Ile Ile Thr 
305                 310                 315                 320 


Phe Lys Ser Gly Glu Thr Phe Gln Val Gly Val Pro Gly Ser Gln His 
                325                 330                 335     


Ile Asp Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu Arg 
            340                 345                 350         


Ile Thr Tyr Leu Thr Glu Thr Lys Ile Asp Lys Leu Cys Val Trp Asn 
        355                 360                 365             


Asn Lys Thr Pro Asn Ser Ile Ala Ala Ile Ser Gly Pro Gly Pro Asn 
    370                 375                 380                 


Asn Thr Tyr Tyr Cys Cys Glu Leu Cys Cys Asn Pro Gln Cys Ala Gly 
385                 390                 395                 400 


Cys Tyr 
        


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  184EcoRV-F 

<400>  10
gtcaggcacc gtgtatgaaa t                                                 21


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  pBREagI-R 

<400>  11
gtccctgatg gtcgtcatct                                                   20


<210>  12
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STaSfcI-F 

<400>  12
gtgaaacaac ctgtaggga                                                    19


<210>  13
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STaEagI-R 

<400>  13
gtggagccgg ccgaaaca                                                     18


<210>  14
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STaHindIII-F 

<400>  14
tgcaaaataa gcttaactaa tctc                                              24


<210>  15
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STaBamH1-R 

<400>  15
gtggagccgg atccaacag                                                    19


<210>  16
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  pSTa11K-F 

<400>  16
gaactttgtt gtaaacctgc ctgt                                              24


<210>  17
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  pSTa11K-R 

<400>  17
acaggcaggt ttacaacaaa gttc                                              24


<210>  18
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  pSTa12F-F 

<400>  18
ctttgttgta attttgcctg tgcc                                              24


<210>  19
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  pSTa12F-R 

<400>  19
ggcacaggca aaattacaac aaag                                              24


<210>  20
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  pSTa13Q-F 

<400>  20
tgttgtaatc ctcagtgtgc tgga                                              24


<210>  21
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  pSTa13Q-R 

<400>  21
tccagcacac tgaggattac aaca                                              24


<210>  22
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  pLT:STa-F 

<400>  22
gcaatcagtg ggccggggcc catgaacaac ttttactgct g                           41


<210>  23
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  pLT:STa-R 

<400>  23
gttgttcatg ggccccggcc cactgattgc cgcaattgaa ttgg                        44


<210>  24
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STa12NS-R 

<400>  24
ataacatcca gcacaggcaa aatt                                              24


<210>  25
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  ST13NS-R 

<400>  25
ataacatcca gcacactgag g                                                 21


<210>  26
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LT-R 

<400>  26
gtttttcata ctgattgccc caattg                                            26


<210>  27
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STa-F 

<400>  27
aacaacacat tttactgctg tg                                                22


<210>  28
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  hLT-F 

<400>  28
atgattgaca tcatgttgca tatagg                                            26


<210>  29
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STaNheI-F 

<400>  29
cgacgtgttt gctagctaa                                                    19


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LTNheI-F 

<400>  30
atcctcgcta gcatgtttta t                                                 21


<210>  31
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LTEagI-R 

<400>  31
gcgtcggccg ctactagttt tccatactga ttgc                                   34


<210>  32
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  pBRNheI-F 

<400>  32
tgctaacgca gtcaggcacc gtgtatg                                           27


<210>  33
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LT192-F 

<400>  33
aattcatcag gaacaattac agg                                               23


<210>  34
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LT192-R 

<400>  34
cctgtaattg ttcctgatga att                                               23


<210>  35
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STa13-F 

<400>  35
ttgtgttgta attttgcttg tacc                                              24


<210>  36
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STa13-R 

<400>  36
ggtacaagca aaattacaac acaa                                              24


<210>  37
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STa13:LT192-F 

<400>  37
gcaatcagtg ggccggggcc catgaatagt agcaattact gc                          42


<210>  38
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LT192:STa13-R 

<400>  38
actattcatg ggccccggcc cactgattgc cgcaattgaa ttgg                        44


<210>  39
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STa13-L-LT192-F 

<400>  39
agtgatcccc gggtaccgag ctcgatgaat agtagcaatt actgc                       45


<210>  40
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STa13-L-LT192-R 

<400>  40
catcgagctc ggtacccggg gatcactgat tgccgcaatt gaattgg                     47


<210>  41
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STa13:LT192-F 

<400>  41
accgggtgct atgggccggg gcccaatggc gacaaattat accgt                       45


<210>  42
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LT192:STa13-R 

<400>  42
gtcgccattg ggccccggcc catagcaccc ggtacaagca aaatt                       45


<210>  43
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LT192A1:STa13-F 

<400>  43
tcatcaggag ggccggtcga catgaatagt agcaattact gctgtg                      46


<210>  44
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STa13:LT192A1-R 

<400>  44
gctactattc atgtcgaccg gccctcctga tgaatttcca caaccttg                    48


<210>  45
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STa13:LT192A2-F 

<400>  45
gggtgctata caattacagg tgatacttgt aatg                                   34


<210>  46
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LT192A2:STa13-R 

<400>  46
cacctgtaat tgtatagcac ccggtacaag caaaattac                              39


<210>  47
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LT192BS:STa13-F 

<400>  47
gcatacggaa tgaatagtag caattactgc                                        30


<210>  48
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STa13:LT192B-R 

<400>  48
actattcatt ccgtatgcac atagagagg                                         29


<210>  49
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STa13:LT192B-F 

<400>  49
gggtgctatg ggccggggcc cgctccccag tctattacag aactatg                     47


<210>  50
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LT192B:STa13-R 

<400>  50
ctggggagcg ggccccggcc catagcaccc ggtacaagca aaattac                     47


<210>  51
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LT192NheI-F3

<400>  51
gtttgctagc aatggcgaca aattatac                                          28


<210>  52
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STa13EagI-R1

<400>  52
gcgacggccg ttattaatag cacccggtac aagc                                   34


<210>  53
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  STa13NheI-F2

<400>  53
gtagctagca tgaatagtag caattac                                           27


<210>  54
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LT192BamHI-R1

<400>  54
gcgtggatcc ctactagttt tccatact                                          28


<210>  55
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LT192A-LL-LT192B-F 

<400>  55
gaattagatc cccgggtacc gagctcggct ccccagtcta ttacag                      46


<210>  56
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LT192A-LL-LT192B-R 

<400>  56
ctggggagcc gagctcggta cccggggatc taattcattc cgaattctg                   49


<210>  57
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LT192A-SL-LT192B-F 

<400>  57
gaattagggc cggggcccgc tccccagtct attacag                                37


<210>  58
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LT192A-SL-LT192B-R 

<400>  58
ctggggagcg ggccccggcc ctaattcatt ccgaattctg                             40


<210>  59
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LT192A-LL-STa13-F 

<400>  59
gaattagatc cccgggtacc gagctcgatg aatagtagca attac                       45


<210>  60
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  LT192A-LL-STa13-R 

<400>  60
actattcatc gagctcggta cccggggatc taattcattc cgaattctg                   49


<210>  61
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  61

Gly Pro Gly Pro 
1               

 

