
                                SEQUENCE LISTING

<110> DEWERCHIN Marianne
      MARTIN Denis
      DEHOTTAY Philippe
      GOFFIN Philippe
      RIOUX Stephane

<120> Expression System
  

<130> VB63702

<160> 45

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 72
<212> DNA
<213> Artifical Sequence

<220> 
<221> misc_signal    
<222> (0)...(0)

<400> 1
atgaaattta gtaaaaaata tatagcagct ggatcagctg ttatcgtatc cttgagtcta 60
tgtgcctatg ca                                                     72

<210> 2
<211> 24
<212> PRT
<213> Artifical Sequence

<220> 
<221> SIGNAL         
<222> (0)...(0)

<400> 2
Met Lys Phe Ser Lys Lys Tyr Ile Ala Ala Gly Ser Ala Val Ile Val
 1               5                  10                  15      
Ser Leu Ser Leu Cys Ala Tyr Ala
            20                  


<210> 3
<211> 75
<212> DNA
<213> Artifical Sequence

<220> 
<221> misc_signal    
<222> (0)...(0)

<400> 3
atgaaaatga ataaaaaggt actattgaca tcgacaatgg cagcttcgct attatcagtc 60
gcaagtgttc aagca                                                  75

<210> 4
<211> 25
<212> PRT
<213> Artifical Sequence

<220> 
<221> SIGNAL         
<222> (0)...(0)

<400> 4
Met Lys Met Asn Lys Lys Val Leu Leu Thr Ser Thr Met Ala Ala Ser
 1               5                  10                  15      
Leu Leu Ser Val Ala Ser Val Gln Ala
            20                  25  


<210> 5
<211> 63
<212> DNA
<213> Artifical Sequence

<220> 
<221> misc_signal    
<222> (0)...(0)

<400> 5
atgaaaaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac cgtagcgcag 60
gcc                                                               63

<210> 6
<211> 21
<212> PRT
<213> Artifical Sequence

<220> 
<221> SIGNAL         
<222> (0)...(0)

<400> 6
Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala
 1               5                  10                  15      
Thr Val Ala Gln Ala
            20      


<210> 7
<211> 57
<212> DNA
<213> Artifical Sequence

<220> 
<221> misc_signal    
<222> (0)...(0)

<400> 7
atgaaaaaag cacttgccac actgattgcc ctcgctctcc cggccgccgc actggcg    57

<210> 8
<211> 19
<212> PRT
<213> Artifical Sequence

<220> 
<221> SIGNAL         
<222> (0)...(0)

<400> 8
Met Lys Lys Ala Leu Ala Thr Leu Ile Ala Leu Ala Leu Pro Ala Ala
 1               5                  10                  15      
Ala Leu Ala
            


<210> 9
<211> 54
<212> DNA
<213> Artifical Sequence

<220> 
<221> misc_signal    
<222> (0)...(0)

<400> 9
atgcgcgtac tgctattttt acttctttcc cttttcatgt tgccggcatt ttcg       54

<210> 10
<211> 18
<212> PRT
<213> Artifical Sequence

<220> 
<221> SIGNAL         
<222> (0)...(0)

<400> 10
Met Arg Val Leu Leu Phe Leu Leu Leu Ser Leu Phe Met Leu Pro Ala
 1               5                  10                  15      
Phe Ser
        


<210> 11
<211> 69
<212> DNA
<213> Artifical Sequence

<220> 
<221> misc_signal    
<222> (0)...(0)

<400> 11
atgatgacta aaataaagtt attgatgctc attatatttt atttaatcat ttcggccagc 60
gcccatgct                                                         69

<210> 12
<211> 23
<212> PRT
<213> Artifical Sequence

<220> 
<221> SIGNAL         
<222> (0)...(0)

<400> 12
Met Met Thr Lys Ile Lys Leu Leu Met Leu Ile Ile Phe Tyr Leu Ile
 1               5                  10                  15      
Ile Ser Ala Ser Ala His Ala
            20              


<210> 13
<211> 75
<212> DNA
<213> Artifical Sequence

<220> 
<221> misc_signal    
<222> (0)...(0)

<400> 13
atgatgaagc acatgcgtat atgggccgtt ctggcatcat ttttagtctt tttttatatt 60
ccgcagagct atgcc                                                  75

<210> 14
<211> 24
<212> PRT
<213> Artifical Sequence

<220> 
<221> SIGNAL         
<222> (0)...(0)

<400> 14
Met Met Lys His Met Arg Ile Trp Ala Val Leu Ala Ser Phe Leu Val Phe
 1               5                  10                  15      
Phe Tyr Ile Pro Gln Ser Tyr Ala
            20                  


<210> 15
<211> 54
<212> DNA
<213> Artifical Sequence

<220> 
<221> misc_signal    
<222> (0)...(0)

<400> 15
atgaggtttt tattgggcgt gctgatgctg atgatctccg gctcagcgct ggcg       54

<210> 16
<211> 18
<212> PRT
<213> Artifical Sequence

<220> 
<221> SIGNAL         
<222> (0)...(0)

<400> 16
Met Arg Phe Leu Leu Gly Val Leu Met Leu Met Ile Ser Gly Ser Ala
 1               5                  10                  15      
Leu Ala
        


<210> 17
<211> 69
<212> DNA
<213> Artifical Sequence

<220> 
<221> misc_signal    
<222> (0)...(0)

<400> 17
atgtcaaaac gaacattcgc ggtgatatta accttgttgt gtagcttctg tattggccag 60
gcgcttgca                                                         69

<210> 18
<211> 23
<212> PRT
<213> Artifical Sequence

<220> 
<221> SIGNAL         
<222> (0)...(0)

<400> 18
Met Ser Lys Arg Thr Phe Ala Val Ile Leu Thr Leu Leu Cys Ser Phe
 1               5                  10                  15      
Cys Ile Gly Gln Ala Leu Ala
            20              


<210> 19
<211> 66
<212> DNA
<213> Artifical Sequence

<220> 
<221> misc_signal    
<222> (0)...(0)

<400> 19
atgatgaagc aggcattacg agtagcattt ggttttctca tactgtgggc atcagttctg 60
catgct                                                            66

<210> 20
<211> 22
<212> PRT
<213> Artifical Sequence

<220> 
<221> SIGNAL         
<222> (0)...(0)

<400> 20
Met Met Lys Gln Ala Leu Arg Val Ala Phe Gly Phe Leu Ile Leu Trp
 1               5                  10                  15      
Ala Ser Val Leu His Ala
            20          


<210> 21
<211> 66
<212> DNA
<213> Artifical Sequence

<220> 
<221> misc_signal    
<222> (0)...(0)

<400> 21
atgctctcca cactccgccg cactctattt gcgctgctgg cttgtgcgtc ttttatcgtc 60
catgcc                                                            66

<210> 22
<211> 22
<212> PRT
<213> Artifical Sequence

<220> 
<221> SIGNAL         
<222> (0)...(0)

<400> 22
Met Leu Ser Thr Leu Arg Arg Thr Leu Phe Ala Leu Leu Ala Cys Ala
 1               5                  10                  15      
Ser Phe Ile Val His Ala
            20          


<210> 23
<211> 57
<212> DNA
<213> Artifical Sequence

<220> 
<221> misc_signal    
<222> (0)...(0)

<400> 23
atgattaaat ttctctctgc attaattctt ctactggtca cgacggcggc tcaggct    57

<210> 24
<211> 19
<212> PRT
<213> Artifical Sequence

<220> 
<221> SIGNAL         
<222> (0)...(0)

<400> 24
Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala
 1               5                  10                  15      
Ala Gln Ala
            


<210> 25
<211> 57
<212> DNA
<213> Artifical Sequence

<220> 
<221> misc_signal    
<222> (0)...(0)

<400> 25
atgaaaaaga tttggctggc gctggctggt ttagttttag cgtttagcgc atcggcg    57

<210> 26
<211> 19
<212> PRT
<213> Artifical Sequence

<220> 
<221> SIGNAL         
<222> (0)...(0)

<400> 26
Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser
 1               5                  10                  15      
Ala Ser Ala
            


<210> 27
<211> 39
<212> DNA
<213> Artifical Sequence

<220> 
<221> primer_bind    
<222> (0)...(0)

<400> 27
gcgcggcata tgggtgcgga tgatgtggtg gatagcagc                        39

<210> 28
<211> 33
<212> DNA
<213> Artifical Sequence

<220> 
<221> primer_bind    
<222> (0)...(0)

<400> 28
gcggagctcg agttattagc ttttgatttc gaa                              33

<210> 29
<211> 90
<212> DNA
<213> Artifical Sequence

<220> 
<221> primer_bind    
<222> (0)...(0)

<400> 29
ggagcgcata tgattaaatt tctctctgca ttaattcttc tactggtcac gacggcggct 60
caggctggtg cggatgatgt ggtggatagc                                  90

<210> 30
<211> 24
<212> DNA
<213> Artifical Sequence

<220> 
<221> primer_bind    
<222> (0)...(0)

<400> 30
cacgccgcat agttcgcacc cgca                                        24

<210> 31
<211> 1605
<212> DNA
<213> c.diphtheriae

<400> 31
ggtgcggatg atgtggtgga tagcagcaaa tcttttgtga tggaaaactt tagcagctat 60
catggcacca aaccgggcta tgtggatagc attcagaaag gcatccagaa accgaaaagc 120
ggcacccagg gcaactatga tgatgattgg aaagaatttt atagcaccga taacaaatat 180
gatgcggcgg gttatagcgt ggataacgaa aatccgctgt ctggcaaagc gggcggtgtg 240
gtgaaagtga cctatccggg cctgaccaaa gtgctggccc tgaaagtgga taacgcggaa 300
accatcaaaa aagaactggg cctgagcctg accgaaccgc tgatggaaca ggtgggcacc 360
gaagaattta ttaaacgctt tggcgatggc gcgagccgtg tggttctgag cctgccgttt 420
gcggaaggca gcagcagcgt ggaatatatt aacaactggg aacaggcgaa agccctgagc 480
gtggaactgg aaattaactt tgaaacccgt ggcaaacgtg gccaggatgc gatgtatgaa 540
tacatggcgc aggcgtgcgc gggcaatcgt gtgcgtcgta gcgtgggcag cagcctgagc 600
tgcattaacc tggattggga cgtcattcgt gataaaacca aaaccaaaat cgaaagcctg 660
aaagaacatg gcccgatcaa aaacaaaatg agcgaaagcc cgaacaaaac cgtgagcgaa 720
gaaaaagcga aacagtatct ggaagaattt catcagaccg cgctggaaca tccggaactg 780
agcgaactga aaaccgtgac cggcaccaat ccggtgtttg cgggtgcgaa ctatgcggcg 840
tgggcggtga atgtggcgca ggtgattgat agcgaaaccg cggataacct ggaaaaaacc 900
accgcggccc tgagcattct gccgggcatt ggcagcgtga tgggcattgc ggatggcgcg 960
gtgcatcata acaccgaaga aattgtggcg cagagcattg ccctgagcag cctgatggtg 1020
gcgcaggcga ttccgctggt tggcgaactg gtggatattg gctttgcggc gtacaacttt 1080
gtggaaagca tcatcaacct gtttcaggtg gtgcataaca gctataaccg tccggcgtat 1140
tctccgggtc ataaaaccca gccgtttctg catgatggct atgcggtgag ctggaacacc 1200
gtggaagata gcattattcg taccggcttt cagggcgaaa gcggccatga tattaaaatt 1260
accgcggaaa acaccccgct gccgattgcg ggtgttctgc tgccgaccat tccgggcaaa 1320
ctggatgtga acaaaagcaa aacccatatt agcgtgaacg gtcgtaaaat tcgtatgcgt 1380
tgccgtgcga ttgatggcga tgtgaccttt tgccgtccga aaagcccggt gtatgtgggc 1440
aacggcgtgc acgcgaacct gcatgtggcg tttcatcgta gcagcagcga aaaaatccat 1500
agcaacgaaa ttagcagcga tagcattggc gtgctgggct atcagaaaac cgtggaccat 1560
accaaagtga actctaaact gagcctgttc ttcgaaatca aaagc                 1605

<210> 32
<211> 535
<212> PRT
<213> c.diphtheriae

<400> 32
Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val Met Glu Asn
 1               5                  10                  15      
Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp Ser Ile Gln
            20                  25                  30          
Lys Gly Ile Gln Lys Pro Lys Ser Gly Thr Gln Gly Asn Tyr Asp Asp
        35                  40                  45              
Asp Trp Lys Glu Phe Tyr Ser Thr Asp Asn Lys Tyr Asp Ala Ala Gly
    50                  55                  60                  
Tyr Ser Val Asp Asn Glu Asn Pro Leu Ser Gly Lys Ala Gly Gly Val
65                  70                  75                  80  
Val Lys Val Thr Tyr Pro Gly Leu Thr Lys Val Leu Ala Leu Lys Val
                85                  90                  95      
Asp Asn Ala Glu Thr Ile Lys Lys Glu Leu Gly Leu Ser Leu Thr Glu
            100                 105                 110         
Pro Leu Met Glu Gln Val Gly Thr Glu Glu Phe Ile Lys Arg Phe Gly
        115                 120                 125             
Asp Gly Ala Ser Arg Val Val Leu Ser Leu Pro Phe Ala Glu Gly Ser
    130                 135                 140                 
Ser Ser Val Glu Tyr Ile Asn Asn Trp Glu Gln Ala Lys Ala Leu Ser
145                 150                 155                 160 
Val Glu Leu Glu Ile Asn Phe Glu Thr Arg Gly Lys Arg Gly Gln Asp
                165                 170                 175     
Ala Met Tyr Glu Tyr Met Ala Gln Ala Cys Ala Gly Asn Arg Val Arg
            180                 185                 190         
Arg Ser Val Gly Ser Ser Leu Ser Cys Ile Asn Leu Asp Trp Asp Val
        195                 200                 205             
Ile Arg Asp Lys Thr Lys Thr Lys Ile Glu Ser Leu Lys Glu His Gly
    210                 215                 220                 
Pro Ile Lys Asn Lys Met Ser Glu Ser Pro Asn Lys Thr Val Ser Glu
225                 230                 235                 240 
Glu Lys Ala Lys Gln Tyr Leu Glu Glu Phe His Gln Thr Ala Leu Glu
                245                 250                 255     
His Pro Glu Leu Ser Glu Leu Lys Thr Val Thr Gly Thr Asn Pro Val
            260                 265                 270         
Phe Ala Gly Ala Asn Tyr Ala Ala Trp Ala Val Asn Val Ala Gln Val
        275                 280                 285             
Ile Asp Ser Glu Thr Ala Asp Asn Leu Glu Lys Thr Thr Ala Ala Leu
    290                 295                 300                 
Ser Ile Leu Pro Gly Ile Gly Ser Val Met Gly Ile Ala Asp Gly Ala
305                 310                 315                 320 
Val His His Asn Thr Glu Glu Ile Val Ala Gln Ser Ile Ala Leu Ser
                325                 330                 335     
Ser Leu Met Val Ala Gln Ala Ile Pro Leu Val Gly Glu Leu Val Asp
            340                 345                 350         
Ile Gly Phe Ala Ala Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu Phe
        355                 360                 365             
Gln Val Val His Asn Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly His
    370                 375                 380                 
Lys Thr Gln Pro Phe Leu His Asp Gly Tyr Ala Val Ser Trp Asn Thr
385                 390                 395                 400 
Val Glu Asp Ser Ile Ile Arg Thr Gly Phe Gln Gly Glu Ser Gly His
                405                 410                 415     
Asp Ile Lys Ile Thr Ala Glu Asn Thr Pro Leu Pro Ile Ala Gly Val
            420                 425                 430         
Leu Leu Pro Thr Ile Pro Gly Lys Leu Asp Val Asn Lys Ser Lys Thr
        435                 440                 445             
His Ile Ser Val Asn Gly Arg Lys Ile Arg Met Arg Cys Arg Ala Ile
    450                 455                 460                 
Asp Gly Asp Val Thr Phe Cys Arg Pro Lys Ser Pro Val Tyr Val Gly
465                 470                 475                 480 
Asn Gly Val His Ala Asn Leu His Val Ala Phe His Arg Ser Ser Ser
                485                 490                 495     
Glu Lys Ile His Ser Asn Glu Ile Ser Ser Asp Ser Ile Gly Val Leu
            500                 505                 510         
Gly Tyr Gln Lys Thr Val Asp His Thr Lys Val Asn Ser Lys Leu Ser
        515                 520                 525             
Leu Phe Phe Glu Ile Lys Ser
    530                 535 


<210> 33
<211> 54
<212> PRT
<213> Artifical Sequence

<220> 

<400> 33
Met Lys Phe Ser Lys Lys Tyr Ile Ala Ala Gly Ser Ala Val Ile Val
 1               5                  10                  15      
Ser Leu Ser Leu Cys Ala Tyr Ala Gly Ala Asp Asp Val Val Asp Ser
            20                  25                  30          
Ser Lys Ser Phe Val Met Glu Asn Phe Ser Ser Tyr His Gly Thr Lys
        35                  40                  45              
Pro Gly Tyr Val Asp Ser
    50                  


<210> 34
<211> 55
<212> PRT
<213> Artifical Sequence

<220> 

<400> 34
Met Lys Met Asn Lys Lys Val Leu Leu Thr Ser Thr Met Ala Ala Ser
 1               5                  10                  15      
Leu Leu Ser Val Ala Ser Val Gln Ala Gly Ala Asp Asp Val Val Asp
            20                  25                  30          
Ser Ser Lys Ser Phe Val Met Glu Asn Phe Ser Ser Tyr His Gly Thr
        35                  40                  45              
Lys Pro Gly Tyr Val Asp Ser
    50                  55  


<210> 35
<211> 51
<212> PRT
<213> Artifical Sequence

<220> 

<400> 35
Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala
 1               5                  10                  15      
Thr Val Ala Gln Ala Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser
            20                  25                  30          
Phe Val Met Glu Asn Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr
        35                  40                  45              
Val Asp Ser
    50      


<210> 36
<211> 49
<212> PRT
<213> Artifical Sequence

<220> 

<400> 36
Met Lys Lys Ala Leu Ala Thr Leu Ile Ala Leu Ala Leu Pro Ala Ala
 1               5                  10                  15      
Ala Leu Ala Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val
            20                  25                  30          
Met Glu Asn Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp
        35                  40                  45              
Ser
    


<210> 37
<211> 48
<212> PRT
<213> Artifical Sequence

<220> 

<400> 37
Met Arg Val Leu Leu Phe Leu Leu Leu Ser Leu Phe Met Leu Pro Ala
 1               5                  10                  15      
Phe Ser Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val Met
            20                  25                  30          
Glu Asn Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp Ser
        35                  40                  45              


<210> 38
<211> 53
<212> PRT
<213> Artifical Sequence

<220> 

<400> 38
Met Met Thr Lys Ile Lys Leu Leu Met Leu Ile Ile Phe Tyr Leu Ile
 1               5                  10                  15      
Ile Ser Ala Ser Ala His Ala Gly Ala Asp Asp Val Val Asp Ser Ser
            20                  25                  30          
Lys Ser Phe Val Met Glu Asn Phe Ser Ser Tyr His Gly Thr Lys Pro
        35                  40                  45              
Gly Tyr Val Asp Ser
    50              


<210> 39
<211> 55
<212> PRT
<213> Artifical Sequence

<220> 

<400> 39
Met Met Lys His Met Arg Ile Trp Ala Val Leu Ala Ser Phe Leu Val
 1               5                  10                  15      
Phe Phe Tyr Ile Pro Gln Ser Tyr Ala Gly Ala Asp Asp Val Val Asp
            20                  25                  30          
Ser Ser Lys Ser Phe Val Met Glu Asn Phe Ser Ser Tyr His Gly Thr
        35                  40                  45              
Lys Pro Gly Tyr Val Asp Ser
    50                  55  


<210> 40
<211> 48
<212> PRT
<213> Artifical Sequence

<220> 

<400> 40
Met Arg Phe Leu Leu Gly Val Leu Met Leu Met Ile Ser Gly Ser Ala
 1               5                  10                  15      
Leu Ala Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val Met
            20                  25                  30          
Glu Asn Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp Ser
        35                  40                  45              


<210> 41
<211> 53
<212> PRT
<213> Artifical Sequence

<220> 

<400> 41
Met Ser Lys Arg Thr Phe Ala Val Ile Leu Thr Leu Leu Cys Ser Phe
 1               5                  10                  15      
Cys Ile Gly Gln Ala Leu Ala Gly Ala Asp Asp Val Val Asp Ser Ser
            20                  25                  30          
Lys Ser Phe Val Met Glu Asn Phe Ser Ser Tyr His Gly Thr Lys Pro
        35                  40                  45              
Gly Tyr Val Asp Ser
    50              


<210> 42
<211> 52
<212> PRT
<213> Artifical Sequence

<220> 

<400> 42
Met Met Lys Gln Ala Leu Arg Val Ala Phe Gly Phe Leu Ile Leu Trp
 1               5                  10                  15      
Ala Ser Val Leu His Ala Gly Ala Asp Asp Val Val Asp Ser Ser Lys
            20                  25                  30          
Ser Phe Val Met Glu Asn Phe Ser Ser Tyr His Gly Thr Lys Pro Gly
        35                  40                  45              
Tyr Val Asp Ser
    50          


<210> 43
<211> 52
<212> PRT
<213> Artifical Sequence

<220> 

<400> 43
Met Leu Ser Thr Leu Arg Arg Thr Leu Phe Ala Leu Leu Ala Cys Ala
 1               5                  10                  15      
Ser Phe Ile Val His Ala Gly Ala Asp Asp Val Val Asp Ser Ser Lys
            20                  25                  30          
Ser Phe Val Met Glu Asn Phe Ser Ser Tyr His Gly Thr Lys Pro Gly
        35                  40                  45              
Tyr Val Asp Ser
    50          


<210> 44
<211> 49
<212> PRT
<213> Artifical Sequence

<220> 

<400> 44
Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala
 1               5                  10                  15      
Ala Gln Ala Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val
            20                  25                  30          
Met Glu Asn Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp
        35                  40                  45              
Ser
    


<210> 45
<211> 49
<212> PRT
<213> Artifical Sequence

<220> 

<400> 45
Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser
 1               5                  10                  15      
Ala Ser Ala Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val
            20                  25                  30          
Met Glu Asn Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp
        35                  40                  45              
Ser
    

