                         SEQUENCE LISTING

<110>  Oslo Universitetssykehus
       Bjrs, Magnar
       Weel-Sneve, Ragnhild
       Kristiansen, Knut Ivan
       Booth, James
 
<120>  Antibacterial polypeptides and use thereof

<130>  45065PC01

<150>  EP 09172106.8
<151>  2009-10-02

<150>  US 61/329,440
<151>  2010-04-29

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  378
<212>  DNA
<213>  Escherichia coli


<220>
<221>  protein_bind
<222>  (6)..(25)
<223>  lexA box 1

<220>
<221>  -35_signal
<222>  (9)..(14)

<220>
<221>  protein_bind
<222>  (28)..(47)
<223>  lexA box 2

<220>
<221>  -10_signal
<222>  (33)..(38)

<220>
<221>  misc_binding
<222>  (132)..(165)
<223>  agrAB repeat

<220>
<221>  terminator
<222>  (338)..(351)

<220>
<221>  terminator
<222>  (356)..(369)

<400>  1
ggacgtgctg gttttataac ctgcatgtac tgtatgatta tccagttagc tctgaggcat       60

tttcactctg gcaatgcgca taaacgcttt caaagtcctg gtcagaagta cgggtggtgc      120

cgttaactga tgctctggcc ggagtgagag agttcttatc taacaatgag acatgcgccg      180

tgacaggcag tggatgagta agcggatgca ttctcactcc atcgcatgga gaaaacgggt      240

gattgataaa gcaatcatcg ttctaggggc gttaattgcg ctgctggaac tgatccgctt      300

tctgcttcag cttctgaact gatagcggaa acgtaattaa gggctaagag cacactactc      360

ttagcccttt aacattta                                                    378


<210>  2
<211>  57
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(57)

<400>  2
atg cgc ata aac gct ttc aaa gtc ctg gtc aga agt acg ggt ggt gcc         48
Met Arg Ile Asn Ala Phe Lys Val Leu Val Arg Ser Thr Gly Gly Ala           
1               5                   10                  15                

gtt aac tga                                                             57
Val Asn                                                                   
                                                                          


<210>  3
<211>  18
<212>  PRT
<213>  Escherichia coli

<400>  3

Met Arg Ile Asn Ala Phe Lys Val Leu Val Arg Ser Thr Gly Gly Ala 
1               5                   10                  15      


Val Asn 
        


<210>  4
<211>  150
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(150)

<400>  4
atg cgc cgt gac agg cag tgg atg agt aag cgg atg cat tct cac tcc         48
Met Arg Arg Asp Arg Gln Trp Met Ser Lys Arg Met His Ser His Ser           
1               5                   10                  15                

atc gca tgg aga aaa cgg gtg att gat aaa gca atc atc gtt cta ggg         96
Ile Ala Trp Arg Lys Arg Val Ile Asp Lys Ala Ile Ile Val Leu Gly           
            20                  25                  30                    

gcg tta att gcg ctg ctg gaa ctg atc cgc ttt ctg ctt cag ctt ctg        144
Ala Leu Ile Ala Leu Leu Glu Leu Ile Arg Phe Leu Leu Gln Leu Leu           
        35                  40                  45                        

aac tga                                                                150
Asn                                                                       
                                                                          


<210>  5
<211>  49
<212>  PRT
<213>  Escherichia coli

<400>  5

Met Arg Arg Asp Arg Gln Trp Met Ser Lys Arg Met His Ser His Ser 
1               5                   10                  15      


Ile Ala Trp Arg Lys Arg Val Ile Asp Lys Ala Ile Ile Val Leu Gly 
            20                  25                  30          


Ala Leu Ile Ala Leu Leu Glu Leu Ile Arg Phe Leu Leu Gln Leu Leu 
        35                  40                  45              


Asn 
    


<210>  6
<211>  129
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(129)

<400>  6
atg agt aag cgg atg cat tct cac tcc atc gca tgg aga aaa cgg gtg         48
Met Ser Lys Arg Met His Ser His Ser Ile Ala Trp Arg Lys Arg Val           
1               5                   10                  15                

att gat aaa gca atc atc gtt cta ggg gcg tta att gcg ctg ctg gaa         96
Ile Asp Lys Ala Ile Ile Val Leu Gly Ala Leu Ile Ala Leu Leu Glu           
            20                  25                  30                    

ctg atc cgc ttt ctg ctt cag ctt ctg aac tga                            129
Leu Ile Arg Phe Leu Leu Gln Leu Leu Asn                                   
        35                  40                                            


<210>  7
<211>  42
<212>  PRT
<213>  Escherichia coli

<400>  7

Met Ser Lys Arg Met His Ser His Ser Ile Ala Trp Arg Lys Arg Val 
1               5                   10                  15      


Ile Asp Lys Ala Ile Ile Val Leu Gly Ala Leu Ile Ala Leu Leu Glu 
            20                  25                  30          


Leu Ile Arg Phe Leu Leu Gln Leu Leu Asn 
        35                  40          


<210>  8
<211>  117
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(117)

<400>  8
atg cat tct cac tcc atc gca tgg aga aaa cgg gtg att gat aaa gca         48
Met His Ser His Ser Ile Ala Trp Arg Lys Arg Val Ile Asp Lys Ala           
1               5                   10                  15                

atc atc gtt cta ggg gcg tta att gcg ctg ctg gaa ctg atc cgc ttt         96
Ile Ile Val Leu Gly Ala Leu Ile Ala Leu Leu Glu Leu Ile Arg Phe           
            20                  25                  30                    

ctg ctt cag ctt ctg aac tga                                            117
Leu Leu Gln Leu Leu Asn                                                   
        35                                                                


<210>  9
<211>  38
<212>  PRT
<213>  Escherichia coli

<400>  9

Met His Ser His Ser Ile Ala Trp Arg Lys Arg Val Ile Asp Lys Ala 
1               5                   10                  15      


Ile Ile Val Leu Gly Ala Leu Ile Ala Leu Leu Glu Leu Ile Arg Phe 
            20                  25                  30          


Leu Leu Gln Leu Leu Asn 
        35              


<210>  10
<211>  84
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(84)

<400>  10
gtg att gat aaa gca atc atc gtt cta ggg gcg tta att gcg ctg ctg         48
Val Ile Asp Lys Ala Ile Ile Val Leu Gly Ala Leu Ile Ala Leu Leu           
1               5                   10                  15                

gaa ctg atc cgc ttt ctg ctt cag ctt ctg aac tga                         84
Glu Leu Ile Arg Phe Leu Leu Gln Leu Leu Asn                               
            20                  25                                        


<210>  11
<211>  27
<212>  PRT
<213>  Escherichia coli

<400>  11

Val Ile Asp Lys Ala Ile Ile Val Leu Gly Ala Leu Ile Ala Leu Leu 
1               5                   10                  15      


Glu Leu Ile Arg Phe Leu Leu Gln Leu Leu Asn 
            20                  25          


<210>  12
<211>  85
<212>  DNA
<213>  Escherichia coli

<400>  12
gttagatgat ggctatctca ctccagtcag agccaccaac tcagggctgg aaagtaaaaa       60

accgacgcaa agtcggtttt tttac                                             85


<210>  13
<211>  86
<212>  DNA
<213>  Escherichia coli

<400>  13
tgttagataa gaactctctc actccagcca gagccaccaa ctcagggctg gaaagtaaaa       60

aaccgacgca aagtcggttt ttttac                                            86


<210>  14
<211>  330
<212>  DNA
<213>  Escherichia coli

<400>  14
ttagctctga ggcattttca ctctggcaat gcgcataaac gctttcaaag tcctggtcag       60

aagtacgggt ggtgccgtta actgatgctc tggccggagt gagagagttc ttatctaaca      120

atgagacatg cgccgtgaca ggcagtggat gagtaagcgg atgcattctc actccatcgc      180

atggagaaaa cgggtgattg ataaagcaat catcgttcta ggggcgttaa ttgcgctgct      240

ggaactgatc cgctttctgc ttcagcttct gaactgatag cggaaacgta attaagggct      300

aagagcacac tactcttagc cctttaacat                                       330


<210>  15
<211>  287
<212>  DNA
<213>  Escherichia coli

<400>  15
ttcaaagtcc tggtcagaag tacgggtggt gccgttaact gatgctctgg ccggagtgag       60

agagttctta tctaacaatg agacatgcgc cgtgacaggc agtggatgag taagcggatg      120

cattctcact ccatcgcatg gagaaaacgg gtgattgata aagcaatcat cgttctaggg      180

gcgttaattg cgctgctgga actgatccgc tttctgcttc agcttctgaa ctgatagcgg      240

aaacgtaatt aagggctaag agcacactac tcttagccct ttaacat                    287


<210>  16
<211>  209
<212>  DNA
<213>  Escherichia coli

<400>  16
tgagacatgc gccgtgacag gcagtggatg agtaagcgga tgcattctca ctccatcgca       60

tggagaaaac gggtgattga taaagcaatc atcgttctag gggcgttaat tgcgctgctg      120

gaactgatcc gctttctgct tcagcttctg aactgatagc ggaaacgtaa ttaagggcta      180

agagcacact actcttagcc ctttaacat                                        209


<210>  17
<211>  27
<212>  PRT
<213>  Escherichia coli

<400>  17

Met Ile Asp Lys Ala Ile Ile Val Leu Gly Ala Leu Ile Ala Leu Leu 
1               5                   10                  15      


Glu Leu Ile Arg Phe Leu Leu Gln Leu Leu Asn 
            20                  25          


<210>  18
<211>  3094
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid pORF5

<400>  18
agcttccaca cattatattg ttatccgctc acaatgtcaa ttggaaattt aaaataattt       60

tctgaggatc cactagttct agagcggccg ccaccgcggt ggctgcatta atgaatcggc      120

caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac      180

tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata      240

cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa      300

aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct      360

gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa      420

agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg      480

cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca      540

cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa      600

ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg      660

gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg      720

tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg      780

acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc      840

tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag      900

attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac      960

gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc     1020

ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag     1080

taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt     1140

ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag     1200

ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca     1260

gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact     1320

ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca     1380

gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg     1440

tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc     1500

atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg     1560

gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca     1620

tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt     1680

atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc     1740

agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc     1800

ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca     1860

tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa     1920

aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat     1980

tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa     2040

aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg     2100

ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat     2160

aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg     2220

ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc     2280

gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt     2340

tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag     2400

cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg     2460

gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc     2520

ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag     2580

ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa     2640

ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca     2700

gtgagcgcgc gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgacg     2760

cgtaaaaaaa cccgccgggg cgggtttttt tacgcgtcga ggaagtgccg gatccgaaaa     2820

gaagaactaa ctcgttgtgg agaataacaa aaatggtcat ctggagctta caggtggcca     2880

ttcgtgggac agtatccctg acagcctaca aaacgcaatt gaagaacgcg aggcatcgtc     2940

ttaacgaggc accgaggcgt cgcattcttc agatggttca acccttaagc ggatcctcag     3000

ttcagaagct gaagcagaaa gcggatcagt tccagcagcg caattaacgc ccctagaacg     3060

atgattgctt tatcaaccat ccgttttcct ccta                                 3094


<210>  19
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G10K variant of dinQ ORF V peptide

<400>  19

Met Ile Asp Lys Ala Ile Ile Val Leu Lys Ala Leu Ile Ala Leu Leu 
1               5                   10                  15      


Glu Leu Ile Arg Phe Leu Leu Gln Leu Leu Asn 
            20                  25          


<210>  20
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  L23I variant of dinQ ORF V peptide

<400>  20

Met Ile Asp Lys Ala Ile Ile Val Leu Gly Ala Leu Ile Ala Leu Leu 
1               5                   10                  15      


Glu Leu Ile Arg Phe Leu Ile Gln Leu Leu Asn 
            20                  25          


<210>  21
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  I7V variant of dinQ ORF V peptide

<400>  21

Met Ile Asp Lys Ala Ile Val Val Leu Gly Ala Leu Ile Ala Leu Leu 
1               5                   10                  15      


Glu Leu Ile Arg Phe Leu Leu Gln Leu Leu Asn 
            20                  25          


<210>  22
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  double mutant variant (L12I,I13V) of dinQ ORF V peptide

<400>  22

Met Ile Asp Lys Ala Ile Ile Val Leu Gly Ala Ile Val Ala Leu Leu 
1               5                   10                  15      


Glu Leu Ile Arg Phe Leu Leu Gln Leu Leu Asn 
            20                  25          


