                         SEQUENCE LISTING

<110>  BASF SE
 
<120>  Neue Amidase und ihre Verwendung zur  Herstellung von 
       3-Aminocarbonsureestern

<130>  PF 000062595 

<160>  4     

<170>  PatentIn version 3.4

<210>  1
<211>  1419
<212>  DNA
<213>  Rhodococcus equi


<220>
<221>  CDS
<222>  (1)..(1419)

<400>  1
atg agt aca tcg gat ccg ggt tgg atg ccg gcg acc gag atg gcc gcc       48
Met Ser Thr Ser Asp Pro Gly Trp Met Pro Ala Thr Glu Met Ala Ala         
1               5                   10                  15              

cag gtc gcc tcg aag aga ttg tcg ccc aac gag atc gca gag gag atg       96
Gln Val Ala Ser Lys Arg Leu Ser Pro Asn Glu Ile Ala Glu Glu Met         
            20                  25                  30                  

atc cgt cga gtc gag gag gtc aat ccc tcc gtc aac gcg atc gtg cac      144
Ile Arg Arg Val Glu Glu Val Asn Pro Ser Val Asn Ala Ile Val His         
        35                  40                  45                      

ttc gac gcc gag cag gtg cgc cgc gac gcc gcc gac ctg gca cgg gcg      192
Phe Asp Ala Glu Gln Val Arg Arg Asp Ala Ala Asp Leu Ala Arg Ala         
    50                  55                  60                          

cag gag agc ggt gag acg ctc ggt ccg ctg cac ggc gtc ccg ttc acg      240
Gln Glu Ser Gly Glu Thr Leu Gly Pro Leu His Gly Val Pro Phe Thr         
65                  70                  75                  80          

atc aag gac ctc acc gac gtc cgt ggc ctg ccg acc acg ttc ggc ctg      288
Ile Lys Asp Leu Thr Asp Val Arg Gly Leu Pro Thr Thr Phe Gly Leu         
                85                  90                  95              

aag ccc atg cgc gac aac atc gcc gaa cgc gac gcg gtg atc gtc acc      336
Lys Pro Met Arg Asp Asn Ile Ala Glu Arg Asp Ala Val Ile Val Thr         
            100                 105                 110                 

cgg ttg cgg cag gcc ggc ggc ctc tac ctc ggc aag acc aac acc ccg      384
Arg Leu Arg Gln Ala Gly Gly Leu Tyr Leu Gly Lys Thr Asn Thr Pro         
        115                 120                 125                     

gag agc ggc tac tac ggc ggc acc gac aac cac ctg ttc ggc ccg acc      432
Glu Ser Gly Tyr Tyr Gly Gly Thr Asp Asn His Leu Phe Gly Pro Thr         
    130                 135                 140                         

cac aat ccg tgg aag ccc ggc cac acc ccc ggc gga tcg agc ggt ggc      480
His Asn Pro Trp Lys Pro Gly His Thr Pro Gly Gly Ser Ser Gly Gly         
145                 150                 155                 160         

gcc gcc gcg gcc gtc gcc gcc ggt ctc ggt ccg ctc gcc gag ggc agc      528
Ala Ala Ala Ala Val Ala Ala Gly Leu Gly Pro Leu Ala Glu Gly Ser         
                165                 170                 175             

gac ggc gcc ggg tcg gtc cgg atc ccg tcg gca ttg tgc ggc gtc gtc      576
Asp Gly Ala Gly Ser Val Arg Ile Pro Ser Ala Leu Cys Gly Val Val         
            180                 185                 190                 

gga ctc aag ccc acg acc ggc gtg atc ccg cag acg atc ctg ccc ggc      624
Gly Leu Lys Pro Thr Thr Gly Val Ile Pro Gln Thr Ile Leu Pro Gly         
        195                 200                 205                     

cgc tac aac aac tgg gcg tac cac ggt ccc atc acc cgc acc gtt gcc      672
Arg Tyr Asn Asn Trp Ala Tyr His Gly Pro Ile Thr Arg Thr Val Ala         
    210                 215                 220                         

gac aac gcc ctg atg ctg gac gtt ctt gcc ggg ccg gat cat tcg gat      720
Asp Asn Ala Leu Met Leu Asp Val Leu Ala Gly Pro Asp His Ser Asp         
225                 230                 235                 240         

ccg ctg agc atc gaa cga gtc gag ggc tcg tac gtc gag gcg gcg cgg      768
Pro Leu Ser Ile Glu Arg Val Glu Gly Ser Tyr Val Glu Ala Ala Arg         
                245                 250                 255             

ggt ggg atc gac ggg ctg cga gtc gcg tgg tcg ccg gac ctc ggt ctc      816
Gly Gly Ile Asp Gly Leu Arg Val Ala Trp Ser Pro Asp Leu Gly Leu         
            260                 265                 270                 

ggc cac gtc gag ccc gac gtc gcc gcg gtc tgc gcc gag gcg gtc gcg      864
Gly His Val Glu Pro Asp Val Ala Ala Val Cys Ala Glu Ala Val Ala         
        275                 280                 285                     

tgt ttc gag gag atg ggc gcc aag gtc gtc gag gcg acg ccg gac tgg      912
Cys Phe Glu Glu Met Gly Ala Lys Val Val Glu Ala Thr Pro Asp Trp         
    290                 295                 300                         

ggc gat ccg tcg gag gcg atg tgg cac ggc atc tgg gtg ccg ggc ttc      960
Gly Asp Pro Ser Glu Ala Met Trp His Gly Ile Trp Val Pro Gly Phe         
305                 310                 315                 320         

gcc ggc gag cac gac atg ctc gac tgg gac gcg ctg cac ggc cag gtc     1008
Ala Gly Glu His Asp Met Leu Asp Trp Asp Ala Leu His Gly Gln Val         
                325                 330                 335             

gac gag aac ctg atc gaa ctg atc cac gag ggc cgg cga ctc acc ggc     1056
Asp Glu Asn Leu Ile Glu Leu Ile His Glu Gly Arg Arg Leu Thr Gly         
            340                 345                 350                 

gtc gac tac ggc cgc gcc gac acg ttc cgc ggt ggc atg tgg gac acc     1104
Val Asp Tyr Gly Arg Ala Asp Thr Phe Arg Gly Gly Met Trp Asp Thr         
        355                 360                 365                     

tgg acc gag ttc atg aac gac tac gac gtc ctg atc tcg ccg acg ctg     1152
Trp Thr Glu Phe Met Asn Asp Tyr Asp Val Leu Ile Ser Pro Thr Leu         
    370                 375                 380                         

gcg tcc gcc acg ttc ccg ctc acc cag ttc gcg ccg gac tgg cta cag     1200
Ala Ser Ala Thr Phe Pro Leu Thr Gln Phe Ala Pro Asp Trp Leu Gln         
385                 390                 395                 400         

ggc aag tcg ctg cgc gag caa ctg ctc gac tgg ctg ctg acc tac ccg     1248
Gly Lys Ser Leu Arg Glu Gln Leu Leu Asp Trp Leu Leu Thr Tyr Pro         
                405                 410                 415             

tac aac atg ctg aac aac ccc gcg atc acg gtc ccc gca ggc ttc acc     1296
Tyr Asn Met Leu Asn Asn Pro Ala Ile Thr Val Pro Ala Gly Phe Thr         
            420                 425                 430                 

ccg gac ggg cgc ccg gtc gga ctc cag atc gcg gcg cgc cat cgt cag     1344
Pro Asp Gly Arg Pro Val Gly Leu Gln Ile Ala Ala Arg His Arg Gln         
        435                 440                 445                     

gac gcg ctc gtg ctg cgg gtc gcg gcg aac ctg gag cag gca cgg ccg     1392
Asp Ala Leu Val Leu Arg Val Ala Ala Asn Leu Glu Gln Ala Arg Pro         
    450                 455                 460                         

tgg gcg gat cgt cga ccg gtg gcg tag                                 1419
Trp Ala Asp Arg Arg Pro Val Ala                                         
465                 470                                                 


<210>  2
<211>  472
<212>  PRT
<213>  Rhodococcus equi

<400>  2

Met Ser Thr Ser Asp Pro Gly Trp Met Pro Ala Thr Glu Met Ala Ala 
1               5                   10                  15      


Gln Val Ala Ser Lys Arg Leu Ser Pro Asn Glu Ile Ala Glu Glu Met 
            20                  25                  30          


Ile Arg Arg Val Glu Glu Val Asn Pro Ser Val Asn Ala Ile Val His 
        35                  40                  45              


Phe Asp Ala Glu Gln Val Arg Arg Asp Ala Ala Asp Leu Ala Arg Ala 
    50                  55                  60                  


Gln Glu Ser Gly Glu Thr Leu Gly Pro Leu His Gly Val Pro Phe Thr 
65                  70                  75                  80  


Ile Lys Asp Leu Thr Asp Val Arg Gly Leu Pro Thr Thr Phe Gly Leu 
                85                  90                  95      


Lys Pro Met Arg Asp Asn Ile Ala Glu Arg Asp Ala Val Ile Val Thr 
            100                 105                 110         


Arg Leu Arg Gln Ala Gly Gly Leu Tyr Leu Gly Lys Thr Asn Thr Pro 
        115                 120                 125             


Glu Ser Gly Tyr Tyr Gly Gly Thr Asp Asn His Leu Phe Gly Pro Thr 
    130                 135                 140                 


His Asn Pro Trp Lys Pro Gly His Thr Pro Gly Gly Ser Ser Gly Gly 
145                 150                 155                 160 


Ala Ala Ala Ala Val Ala Ala Gly Leu Gly Pro Leu Ala Glu Gly Ser 
                165                 170                 175     


Asp Gly Ala Gly Ser Val Arg Ile Pro Ser Ala Leu Cys Gly Val Val 
            180                 185                 190         


Gly Leu Lys Pro Thr Thr Gly Val Ile Pro Gln Thr Ile Leu Pro Gly 
        195                 200                 205             


Arg Tyr Asn Asn Trp Ala Tyr His Gly Pro Ile Thr Arg Thr Val Ala 
    210                 215                 220                 


Asp Asn Ala Leu Met Leu Asp Val Leu Ala Gly Pro Asp His Ser Asp 
225                 230                 235                 240 


Pro Leu Ser Ile Glu Arg Val Glu Gly Ser Tyr Val Glu Ala Ala Arg 
                245                 250                 255     


Gly Gly Ile Asp Gly Leu Arg Val Ala Trp Ser Pro Asp Leu Gly Leu 
            260                 265                 270         


Gly His Val Glu Pro Asp Val Ala Ala Val Cys Ala Glu Ala Val Ala 
        275                 280                 285             


Cys Phe Glu Glu Met Gly Ala Lys Val Val Glu Ala Thr Pro Asp Trp 
    290                 295                 300                 


Gly Asp Pro Ser Glu Ala Met Trp His Gly Ile Trp Val Pro Gly Phe 
305                 310                 315                 320 


Ala Gly Glu His Asp Met Leu Asp Trp Asp Ala Leu His Gly Gln Val 
                325                 330                 335     


Asp Glu Asn Leu Ile Glu Leu Ile His Glu Gly Arg Arg Leu Thr Gly 
            340                 345                 350         


Val Asp Tyr Gly Arg Ala Asp Thr Phe Arg Gly Gly Met Trp Asp Thr 
        355                 360                 365             


Trp Thr Glu Phe Met Asn Asp Tyr Asp Val Leu Ile Ser Pro Thr Leu 
    370                 375                 380                 


Ala Ser Ala Thr Phe Pro Leu Thr Gln Phe Ala Pro Asp Trp Leu Gln 
385                 390                 395                 400 


Gly Lys Ser Leu Arg Glu Gln Leu Leu Asp Trp Leu Leu Thr Tyr Pro 
                405                 410                 415     


Tyr Asn Met Leu Asn Asn Pro Ala Ile Thr Val Pro Ala Gly Phe Thr 
            420                 425                 430         


Pro Asp Gly Arg Pro Val Gly Leu Gln Ile Ala Ala Arg His Arg Gln 
        435                 440                 445             


Asp Ala Leu Val Leu Arg Val Ala Ala Asn Leu Glu Gln Ala Arg Pro 
    450                 455                 460                 


Trp Ala Asp Arg Arg Pro Val Ala 
465                 470         


<210>  3
<211>  1431
<212>  DNA
<213>  Rhodococcus erythropolis


<220>
<221>  CDS
<222>  (1)..(1431)

<400>  3
atg acc gaa cag aat ctg cat tgg ctc tcc gct acc gag atg gcg gcg       48
Met Thr Glu Gln Asn Leu His Trp Leu Ser Ala Thr Glu Met Ala Ala         
1               5                   10                  15              

tcg gtc gcg tcg aac agt ctc tcg ccc aac gag att gcc gaa gcg atg       96
Ser Val Ala Ser Asn Ser Leu Ser Pro Asn Glu Ile Ala Glu Ala Met         
            20                  25                  30                  

atc cag cgc gtc gac gct gtc aat ccg tcg atc aac gcg ata gtg cag      144
Ile Gln Arg Val Asp Ala Val Asn Pro Ser Ile Asn Ala Ile Val Gln         
        35                  40                  45                      

ttc gat cgc gag cag gtc acg cgc gac gcg gcc gaa ctc tca cgg caa      192
Phe Asp Arg Glu Gln Val Thr Arg Asp Ala Ala Glu Leu Ser Arg Gln         
    50                  55                  60                          

cag gaa tcg ggc gag aaa ctc ggc ccg ctg cac ggc gtt ccg ttc acg      240
Gln Glu Ser Gly Glu Lys Leu Gly Pro Leu His Gly Val Pro Phe Thr         
65                  70                  75                  80          

atc aaa gat ctg acg gca gtc gac ggg ctg ccg acc acg ttc ggg atg      288
Ile Lys Asp Leu Thr Ala Val Asp Gly Leu Pro Thr Thr Phe Gly Met         
                85                  90                  95              

aag ccg atg gcc gac aac atc gcg acg gga aat gcc gtc gtc gtg gac      336
Lys Pro Met Ala Asp Asn Ile Ala Thr Gly Asn Ala Val Val Val Asp         
            100                 105                 110                 

agg ctg cgc ggc gcc ggc gga ctg ttc ctg gga aag acg aac act ccc      384
Arg Leu Arg Gly Ala Gly Gly Leu Phe Leu Gly Lys Thr Asn Thr Pro         
        115                 120                 125                     

gaa agc ggt tac tac ggt ggc acg gac aac cac ctg tac ggg ccg acg      432
Glu Ser Gly Tyr Tyr Gly Gly Thr Asp Asn His Leu Tyr Gly Pro Thr         
    130                 135                 140                         

cac aac ccg tgg aag ctc ggc aac agc gcg ggc ggg tcc agt ggc ggc      480
His Asn Pro Trp Lys Leu Gly Asn Ser Ala Gly Gly Ser Ser Gly Gly         
145                 150                 155                 160         

gcg tcg gct gcc gtg gct gca ggc ctc ggg cca ctt gcc gag ggc agt      528
Ala Ser Ala Ala Val Ala Ala Gly Leu Gly Pro Leu Ala Glu Gly Ser         
                165                 170                 175             

gac ggc gcc gga tcg gtg cgt atc cca tcg gcg ctc tgc ggg gtc gtc      576
Asp Gly Ala Gly Ser Val Arg Ile Pro Ser Ala Leu Cys Gly Val Val         
            180                 185                 190                 

ggg ctc aaa ccg acc acc ggc gtc att ccg cag acc att ctg gcc ggg      624
Gly Leu Lys Pro Thr Thr Gly Val Ile Pro Gln Thr Ile Leu Ala Gly         
        195                 200                 205                     

cgg ttc tac aac tgg gcg tac cac ggt ccg atc acc agg acc gtc gcc      672
Arg Phe Tyr Asn Trp Ala Tyr His Gly Pro Ile Thr Arg Thr Val Ala         
    210                 215                 220                         

gac aac gcg ctc atg ctc gac atc atg gcc ggg ccg gac aat gcg gat      720
Asp Asn Ala Leu Met Leu Asp Ile Met Ala Gly Pro Asp Asn Ala Asp         
225                 230                 235                 240         

ccg ctc tcg atc gag cgt gcg gag acc tcg tac gtc gaa gcg gcg aag      768
Pro Leu Ser Ile Glu Arg Ala Glu Thr Ser Tyr Val Glu Ala Ala Lys         
                245                 250                 255             

ggt gac gtg aag ggg ctt cgc gtc gcg tgg tcg acg aat ctc ggc ctc      816
Gly Asp Val Lys Gly Leu Arg Val Ala Trp Ser Thr Asn Leu Gly Leu         
            260                 265                 270                 

ggc cat gtt gat ccg gag gtg ctg gcg gtg tgc ctc gac gcg ctg gcg      864
Gly His Val Asp Pro Glu Val Leu Ala Val Cys Leu Asp Ala Leu Ala         
        275                 280                 285                     

gca ttc gag gaa ttg ggt gcc cag atc acc gag gcg acc ccg cag tgg      912
Ala Phe Glu Glu Leu Gly Ala Gln Ile Thr Glu Ala Thr Pro Gln Trp         
    290                 295                 300                         

gga aat ccg tcg gag tcg atg tgg aac ggc atc tgg gtt ccc ggt ttc      960
Gly Asn Pro Ser Glu Ser Met Trp Asn Gly Ile Trp Val Pro Gly Phe         
305                 310                 315                 320         

gct tcc gaa tac gac ttg ctc gac tgg gag aac cag cgc ggc gag gtc     1008
Ala Ser Glu Tyr Asp Leu Leu Asp Trp Glu Asn Gln Arg Gly Glu Val         
                325                 330                 335             

gac gac aac ctg atc gag atc atg cac gag gcc gag cgg ctc acc ggt     1056
Asp Asp Asn Leu Ile Glu Ile Met His Glu Ala Glu Arg Leu Thr Gly         
            340                 345                 350                 

gtc gac gtc ggg cgg gcc gac gca ttc cgc ggc gtc atg tgg gac acg     1104
Val Asp Val Gly Arg Ala Asp Ala Phe Arg Gly Val Met Trp Asp Thr         
        355                 360                 365                     

tgg acc acg ttc atg aac gac tac gac gtg ttg gtc tcg ccg acc ttg     1152
Trp Thr Thr Phe Met Asn Asp Tyr Asp Val Leu Val Ser Pro Thr Leu         
    370                 375                 380                         

gct tcg gcc acg ttc ccg ctc agt cag ttc gcg ccg tcg tgg ctc gaa     1200
Ala Ser Ala Thr Phe Pro Leu Ser Gln Phe Ala Pro Ser Trp Leu Glu         
385                 390                 395                 400         

ggt gcg tcg ttg cgt gag cag ttg ctc gat tgg ctc ttc acc tac ccg     1248
Gly Ala Ser Leu Arg Glu Gln Leu Leu Asp Trp Leu Phe Thr Tyr Pro         
                405                 410                 415             

tac aac atg ctc aac aac ccc gcg atc acc gtg ccc gcc gga ttt acc     1296
Tyr Asn Met Leu Asn Asn Pro Ala Ile Thr Val Pro Ala Gly Phe Thr         
            420                 425                 430                 

gcc gac ggt cga ccg gtg ggg ctg cag atc gcc gca cgc cac cgc cag     1344
Ala Asp Gly Arg Pro Val Gly Leu Gln Ile Ala Ala Arg His Arg Gln         
        435                 440                 445                     

gac gca ctg gtt ctg cgg act gcc gcg aac ttc gaa gcg gtg cgt ccg     1392
Asp Ala Leu Val Leu Arg Thr Ala Ala Asn Phe Glu Ala Val Arg Pro         
    450                 455                 460                         

tgg gcg gac agg aag ccg gcc gat tca ctg gtg gtg gcc                 1431
Trp Ala Asp Arg Lys Pro Ala Asp Ser Leu Val Val Ala                     
465                 470                 475                             


<210>  4
<211>  477
<212>  PRT
<213>  Rhodococcus erythropolis

<400>  4

Met Thr Glu Gln Asn Leu His Trp Leu Ser Ala Thr Glu Met Ala Ala 
1               5                   10                  15      


Ser Val Ala Ser Asn Ser Leu Ser Pro Asn Glu Ile Ala Glu Ala Met 
            20                  25                  30          


Ile Gln Arg Val Asp Ala Val Asn Pro Ser Ile Asn Ala Ile Val Gln 
        35                  40                  45              


Phe Asp Arg Glu Gln Val Thr Arg Asp Ala Ala Glu Leu Ser Arg Gln 
    50                  55                  60                  


Gln Glu Ser Gly Glu Lys Leu Gly Pro Leu His Gly Val Pro Phe Thr 
65                  70                  75                  80  


Ile Lys Asp Leu Thr Ala Val Asp Gly Leu Pro Thr Thr Phe Gly Met 
                85                  90                  95      


Lys Pro Met Ala Asp Asn Ile Ala Thr Gly Asn Ala Val Val Val Asp 
            100                 105                 110         


Arg Leu Arg Gly Ala Gly Gly Leu Phe Leu Gly Lys Thr Asn Thr Pro 
        115                 120                 125             


Glu Ser Gly Tyr Tyr Gly Gly Thr Asp Asn His Leu Tyr Gly Pro Thr 
    130                 135                 140                 


His Asn Pro Trp Lys Leu Gly Asn Ser Ala Gly Gly Ser Ser Gly Gly 
145                 150                 155                 160 


Ala Ser Ala Ala Val Ala Ala Gly Leu Gly Pro Leu Ala Glu Gly Ser 
                165                 170                 175     


Asp Gly Ala Gly Ser Val Arg Ile Pro Ser Ala Leu Cys Gly Val Val 
            180                 185                 190         


Gly Leu Lys Pro Thr Thr Gly Val Ile Pro Gln Thr Ile Leu Ala Gly 
        195                 200                 205             


Arg Phe Tyr Asn Trp Ala Tyr His Gly Pro Ile Thr Arg Thr Val Ala 
    210                 215                 220                 


Asp Asn Ala Leu Met Leu Asp Ile Met Ala Gly Pro Asp Asn Ala Asp 
225                 230                 235                 240 


Pro Leu Ser Ile Glu Arg Ala Glu Thr Ser Tyr Val Glu Ala Ala Lys 
                245                 250                 255     


Gly Asp Val Lys Gly Leu Arg Val Ala Trp Ser Thr Asn Leu Gly Leu 
            260                 265                 270         


Gly His Val Asp Pro Glu Val Leu Ala Val Cys Leu Asp Ala Leu Ala 
        275                 280                 285             


Ala Phe Glu Glu Leu Gly Ala Gln Ile Thr Glu Ala Thr Pro Gln Trp 
    290                 295                 300                 


Gly Asn Pro Ser Glu Ser Met Trp Asn Gly Ile Trp Val Pro Gly Phe 
305                 310                 315                 320 


Ala Ser Glu Tyr Asp Leu Leu Asp Trp Glu Asn Gln Arg Gly Glu Val 
                325                 330                 335     


Asp Asp Asn Leu Ile Glu Ile Met His Glu Ala Glu Arg Leu Thr Gly 
            340                 345                 350         


Val Asp Val Gly Arg Ala Asp Ala Phe Arg Gly Val Met Trp Asp Thr 
        355                 360                 365             


Trp Thr Thr Phe Met Asn Asp Tyr Asp Val Leu Val Ser Pro Thr Leu 
    370                 375                 380                 


Ala Ser Ala Thr Phe Pro Leu Ser Gln Phe Ala Pro Ser Trp Leu Glu 
385                 390                 395                 400 


Gly Ala Ser Leu Arg Glu Gln Leu Leu Asp Trp Leu Phe Thr Tyr Pro 
                405                 410                 415     


Tyr Asn Met Leu Asn Asn Pro Ala Ile Thr Val Pro Ala Gly Phe Thr 
            420                 425                 430         


Ala Asp Gly Arg Pro Val Gly Leu Gln Ile Ala Ala Arg His Arg Gln 
        435                 440                 445             


Asp Ala Leu Val Leu Arg Thr Ala Ala Asn Phe Glu Ala Val Arg Pro 
    450                 455                 460                 


Trp Ala Asp Arg Lys Pro Ala Asp Ser Leu Val Val Ala 
465                 470                 475         


