                         SEQUENCE LISTING

<110>  University of Idaho
       Hrdlicka, Patrick
       Kumar, Pawan
       Ostergaard, Michael E.
 
<120>  NUCLEOBASE-FUNCTIONALIZED CONFORMATIONALLY RESTRICTED NUCLEOTIDES AND OLIGONUCLEOTIDES FOR TARGETING OF NUCLEIC ACIDS

<130>  7832-85502-01

<150>  US61/241,292
<151>  2009-09-10

<160>  48    

<170>  PatentIn version 3.5

<210>  1
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  1
tttttntttn tntnt                                                        15


<210>  2
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthesized dsDNA sequence (5')

<400>  2
gctaaaaaga aagagagatc g                                                 21


<210>  3
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthesized dsDNA target (3')

<400>  3
cgatttttct ttctctctac g                                                 21


<210>  4
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthesized mismatched DNA target5'


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, t, c, or g

<400>  4
gctaaanaga aagagagatc g                                                 21


<210>  5
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  modified dsDNA target3'


<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n is t, a, or c

<400>  5
cgatttttct ntctctctac g                                                 21


<210>  6
<211>  13
<212>  DNA
<213>  Artificial sequence

<220>
<223>  unmodified DNA target for fluorescence studies

<400>  6
cgcaactcaa cgc                                                          13


<210>  7
<211>  13
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthezied DNA sequence modified with compound P


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       Beta-D-3-(2,4-dioxo-5-(3-(pyrene-1-carboxamido)prop-1-ynyl)-3,4-d
       ihydropyrimidin-1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<400>  7
cgcaacncaa cgc                                                          13


<210>  8
<211>  13
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthesized DNA sequence modified with compound P'


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       Alpha-L-3-(2,4-dioxo-5-(3-(pyrene-1-carboxamido)prop-1-ynyl)-3,4-
       dihydropyrimidin-1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<400>  8
cgcaacncaa cgc                                                          13


<210>  9
<211>  13
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthesized DNA sequence modified with compound Beta


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       Beta-D-3-(2,4-dioxo-5-(1-(pyren-1-ylmethyl)-1H-1,2,3-triazol-4-yl
       )-3,4-dihydropyrimidin-1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<400>  9
cgcaacncaa cgc                                                          13


<210>  10
<211>  13
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthesized  DNA sequence modified with compound gamma


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       3-(2,4-dioxo-5-(1-(pyren-1-yl)-1H-1,2,3-triazol-4-yl)-3,4-dihydro
       pyrimidin-1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       Beta-D-3-(2,4-dioxo-5-(1-(pyren-1-yl)-1H-1,2,3-triazol-4-yl)-3,4-
       dihydropyrimidin-1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<400>  10
cgcaacncaa cgc                                                          13


<210>  11
<211>  13
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthesized DNA sequence modified with compound Alpha


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       3-(2,4-dioxo-5-(perylen-3-ylethynyl)-3,4-dihydropyrimidin-1(2H)-y
       l)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<400>  11
cgcaacncaa cgc                                                          13


<210>  12
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide for dsDNA target modified with 
       compound Y


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is 
       Beta-D-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-((
       hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  12
tttttntntn tntnt                                                        15


<210>  13
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide for dsDNA target modified with 
       compound Y


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is 
       Beta-D-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-((
       hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is 
       Beta-D-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-((
       hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is 
       Beta-D-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-((
       hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  13
ttttnnttnn tnnnt                                                        15


<210>  14
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide for dsDNA target modified with 
       compound Y


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is 
       Beta-D-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-((
       hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is 
       Beta-D-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-((
       hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n is 
       Beta-D-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-((
       hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  14
ttttnntntn nntnt                                                        15


<210>  15
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n is 
       Beta-D-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-((
       hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is 
       Beta-D-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-((
       hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       Beta-D-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-((
       hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is 
       Beta-D-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-((
       hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n is 
       Beta-D-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-((
       hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is 
       Beta-D-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-((
       hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  15
ttntnnntnn nnnnt                                                        15


<210>  16
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide for dsDNA target modified with 
       compound V


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is 
       Beta-D-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-1
       (2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  16
tttttntntn tntnt                                                        15


<210>  17
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide for dsDNA target modified with 
       compound V


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is 
       Beta-D-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-1
       (2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is 
       Beta-D-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-1
       (2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is 
       Beta-D-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-1
       (2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  17
ttttnnttnn tnnnt                                                        15


<210>  18
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide for dsDNA target modified with 
       compound V


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is 
       Beta-D-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-1
       (2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is 
       Beta-D-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-1
       (2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n is 
       Beta-D-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-1
       (2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  18
ttttnntntn nntnt                                                        15


<210>  19
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide for dsDNA target modified with 
       compound V


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n is 
       Beta-D-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-1
       (2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is 
       Beta-D-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-1
       (2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       Beta-D-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-1
       (2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is 
       Beta-D-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-1
       (2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n is 
       Beta-D-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-1
       (2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is 
       Beta-D-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-1
       (2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  19
ttntnnntnn nnnnt                                                        15


<210>  20
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide for dsDNA target modified with 
       compound X


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is Beta-D-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is Beta-D-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is Beta-D-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  20
ttttnnttnn tnnnt                                                        15


<210>  21
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide for dsDNA target modified with 
       compound X


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is Beta-D-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is  5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is Beta-D-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is  5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n is Beta-D-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is  5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is  5-methyldeoxycytidine

<400>  21
ttttnntntn nntnt                                                        15


<210>  22
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide for dsDNA target modified with 
       compound X


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is Beta-D-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is Beta-D-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is Beta-D-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n is Beta-D-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is Beta-D-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  22
ttntnnntnn nnnnt                                                        15


<210>  23
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide for dsDNA target modified with 
       compound X


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is Beta-D-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  23
tttttntntn tntnt                                                        15


<210>  24
<211>  13
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide for fluorescence studies

<400>  24
cgcaaataaa cgc                                                          13


<210>  25
<211>  13
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide for fluorescence studies

<400>  25
cgcaagtgaa cgc                                                          13


<210>  26
<211>  13
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide for fluorescence studies

<400>  26
cgcaatttaa cgc                                                          13


<210>  27
<211>  13
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide modified with compound P''


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       5-(2,4-dioxo-5-(3-(pyrene-1-carboxamido)prop-1-ynyl)-3,4-dihydrop
       yrimidin-1(2H)-yl)-2-((hydrogen 
       phosphonatooxy)methyl)tetrahydrofuran-3-yl phosphate

<400>  27
cgcaaanaaa cgc                                                          13


<210>  28
<211>  13
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide modified with compound P''


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       5-(2,4-dioxo-5-(3-(pyrene-1-carboxamido)prop-1-ynyl)-3,4-dihydrop
       yrimidin-1(2H)-yl)-2-((hydrogen 
       phosphonatooxy)methyl)tetrahydrofuran-3-yl phosphate

<400>  28
cgcaacncaa cgc                                                          13


<210>  29
<211>  13
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide modified with compound P''


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       5-(2,4-dioxo-5-(3-(pyrene-1-carboxamido)prop-1-ynyl)-3,4-dihydrop
       yrimidin-1(2H)-yl)-2-((hydrogen 
       phosphonatooxy)methyl)tetrahydrofuran-3-yl phosphate

<400>  29
cgcaagngaa cgc                                                          13


<210>  30
<211>  13
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide modified with compound P''


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       5-(2,4-dioxo-5-(3-(pyrene-1-carboxamido)prop-1-ynyl)-3,4-dihydrop
       yrimidin-1(2H)-yl)-2-((hydrogen 
       phosphonatooxy)methyl)tetrahydrofuran-3-yl phosphate

<400>  30
cgcaatntaa cgc                                                          13


<210>  31
<211>  13
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide modified with compound P


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       Beta-D-3-(2,4-dioxo-5-(3-(pyrene-1-carboxamido)prop-1-ynyl)-3,4-d
       ihydropyrimidin-1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<400>  31
cgcaaanaaa cgc                                                          13


<210>  32
<211>  13
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide modified with compound P


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       Beta-D-3-(2,4-dioxo-5-(3-(pyrene-1-carboxamido)prop-1-ynyl)-3,4-d
       ihydropyrimidin-1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<400>  32
cgcaagngaa cgc                                                          13


<210>  33
<211>  13
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide modified with compound P


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       Beta-D-3-(2,4-dioxo-5-(3-(pyrene-1-carboxamido)prop-1-ynyl)-3,4-d
       ihydropyrimidin-1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<400>  33
cgcaatntaa cgc                                                          13


<210>  34
<211>  13
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide modified with compound P'


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       Alpha-L-3-(2,4-dioxo-5-(3-(pyrene-1-carboxamido)prop-1-ynyl)-3,4-
       dihydropyrimidin-1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<400>  34
cgcaaanaaa cgc                                                          13


<210>  35
<211>  13
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide modified with compound P'


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       Alpha-L3-(2,4-dioxo-5-(3-(pyrene-1-carboxamido)prop-1-ynyl)-3,4-d
       ihydropyrimidin-1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<400>  35
cgcaagngaa cgc                                                          13


<210>  36
<211>  13
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthesized oligonucleotide modified with compound P'


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       Alpha-L3-(2,4-dioxo-5-(3-(pyrene-1-carboxamido)prop-1-ynyl)-3,4-d
       ihydropyrimidin-1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<400>  36
cgcaatntaa cgc                                                          13


<210>  37
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthesized oligonucleotide modified with compound X'


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is Alpha-L-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  37
tttttntntn tntnt                                                        15


<210>  38
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthesized oligonucleotide modified with compound Y'


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is 
       Alpha-L-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-(
       (hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  38
tttttntntn tntnt                                                        15


<210>  39
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthesized oligonucleotide modified with compound V'


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is 
       Alpha-D-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-
       1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  39
tttttntntn tntnt                                                        15


<210>  40
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide modified with compound X'


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is Alpha-L-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is Alpha-L-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is Alpha-L-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  40
ttttnnttnn tnnnt                                                        15


<210>  41
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic oligonucleotide modified with compound X'


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is Alpha-L-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is  5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is Alpha-L-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is  5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n is Alpha-L-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is  5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is  5-methyldeoxycytidine

<400>  41
ttttnntntn nntnt                                                        15


<210>  42
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic oligonucleotide modified with compound X'


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n is Alpha-L-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is Alpha-L-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is Alpha-L-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is Alpha-L-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n is Alpha-L-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is Alpha-L-1-((hydrogen 
       phosphonatooxy)methyl)-3-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin
       -1(2H)-yl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl phosphate

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  42
ttntnnntnn nnnnt                                                        15


<210>  43
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic oligonucleotide modified with compound Y'


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is 
       Alpha-L-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-(
       (hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is 
       Alpha-L-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-(
       (hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is 
       Alpha-L-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-(
       (hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  43
ttttnnttnn tnnnt                                                        15


<210>  44
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic oligonucleotide modified with compound Y'


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is 
       Alpha-L-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-(
       (hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is 
       Alpha-L-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-(
       (hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n is 
       Alpha-L-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-(
       (hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  44
ttttnntntn nntnt                                                        15


<210>  45
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic oligonucleotide modified with compound Y'


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n is 
       Alpha-L-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-(
       (hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is 
       Alpha-L-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-(
       (hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       Alpha-L-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-(
       (hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is 
       Alpha-L-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-(
       (hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n is 
       Alpha-L-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-(
       (hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is 
       Alpha-L-3-(5-ethynyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-(
       (hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  45
ttntnnntnn nnnnt                                                        15


<210>  46
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic oligonucleotide modified with compound V'


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is 
       Alpha-L-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-
       1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is 
       Alpha-L-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-
       1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is 
       Alpha-L-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-
       1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  46
ttttnnttnn tnnnt                                                        15


<210>  47
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic oligonucleotide modified with compound V'


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is 
       Alpha-L-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-
       1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is 
       Alpha-L-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-
       1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n is 
       Alpha-L-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-
       1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  47
ttttnntntn nntnt                                                        15


<210>  48
<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic oligonucleotide modified with compound V'


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n is 
       Alpha-L-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-
       1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is 
       Alpha-L-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-
       1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is 
       Alpha-L-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-
       1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is 
       Alpha-L-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-
       1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n is 
       Alpha-L-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-
       1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is 5-methyldeoxycytidine

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is 
       Alpha-L-3-(5-(3-aminoprop-1-ynyl)-2,4-dioxo-3,4-dihydropyrimidin-
       1(2H)-yl)-1-((hydrogen 
       phosphonatooxy)methyl)-2,5-dioxabicyclo[2.2.1]heptan-7-yl 
       

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is 5-methyldeoxycytidine

<400>  48
ttntnnntnn nnnnt                                                        15


