                         SEQUENCE LISTING

<110>  Biocon Limited
 
<120>  Novel fusion polypeptides and expression methods thereof

<130>  PCT0916

<150>  02193/CHE/2009
<151>  2009-09-10

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  978
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exon


<220>
<221>  exon
<222>  (1)..(978)

<400>  1
gtt cct gtt tct ggt aaa tct gga tct tcc aac acc gcc gtc tct gca         48
Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala           
1               5                   10                  15                

tct gac aat gct gcc ctc cct cct ctc atc tcc agc cgt tgt gct cct         96
Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro           
            20                  25                  30                    

cct tct aac aag gga agt aaa agc gat ctc caa gct gaa cct tac aac        144
Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn           
        35                  40                  45                        

atg caa aag aat aca gaa tgg tat gag tcc cat ggt ggc aac ctg aca        192
Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr           
    50                  55                  60                            

tcc atc gga aag cgt gat gac aac ttg gtt ggt ggc atg act ttg gac        240
Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp           
65                  70                  75                  80            

tta ccc agc gat gct cct cct atc agc ctc tct agc tct acc aac agc        288
Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser           
                85                  90                  95                

gcc gat gac gat gac aag att gtc gga ggc tac acc tgt ggt gca aac        336
Ala Asp Asp Asp Asp Lys Ile Val Gly Gly Tyr Thr Cys Gly Ala Asn           
            100                 105                 110                   

act gtg ccc tac caa gtg agc ctg aat tca gga tac cac ttt tgc ggt        384
Thr Val Pro Tyr Gln Val Ser Leu Asn Ser Gly Tyr His Phe Cys Gly           
        115                 120                 125                       

ggg tca tta atc aac tcg caa tgg gtt gta tct gcc gca cat tgt tac        432
Gly Ser Leu Ile Asn Ser Gln Trp Val Val Ser Ala Ala His Cys Tyr           
    130                 135                 140                           

aaa tca ggc att caa gtg aga ctt gga gaa gat aat att aat gtc gta        480
Lys Ser Gly Ile Gln Val Arg Leu Gly Glu Asp Asn Ile Asn Val Val           
145                 150                 155                 160           

gag ggt aat gaa cag ttc att agt gcc tct aaa tcc att gtt cat cct        528
Glu Gly Asn Glu Gln Phe Ile Ser Ala Ser Lys Ser Ile Val His Pro           
                165                 170                 175               

tct tat aat tcc aac acc ctt aac aat gat ata atg ttg ata aag cta        576
Ser Tyr Asn Ser Asn Thr Leu Asn Asn Asp Ile Met Leu Ile Lys Leu           
            180                 185                 190                   

aag tcc gct gcc agt ttg aat tct aga gtc gct tct atc tct ctg cca        624
Lys Ser Ala Ala Ser Leu Asn Ser Arg Val Ala Ser Ile Ser Leu Pro           
        195                 200                 205                       

act tcc tgt gca agc gct ggc act caa tgt ttg ata agt ggt tgg gga        672
Thr Ser Cys Ala Ser Ala Gly Thr Gln Cys Leu Ile Ser Gly Trp Gly           
    210                 215                 220                           

aac acg aag agt tcc ggt act agc tac cct gac gtt cta aag tgt ctc        720
Asn Thr Lys Ser Ser Gly Thr Ser Tyr Pro Asp Val Leu Lys Cys Leu           
225                 230                 235                 240           

aaa gct cca atc ttg tca gat tcc tca tgc aaa agt gct tat cct ggt        768
Lys Ala Pro Ile Leu Ser Asp Ser Ser Cys Lys Ser Ala Tyr Pro Gly           
                245                 250                 255               

cag atc aca tcg aac atg ttt tgt gct ggt tac ttg gag gga ggg aag        816
Gln Ile Thr Ser Asn Met Phe Cys Ala Gly Tyr Leu Glu Gly Gly Lys           
            260                 265                 270                   

gat tct tgt cag gga gac tct gga ggt cca gtt gtc tgt tct gga aaa        864
Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro Val Val Cys Ser Gly Lys           
        275                 280                 285                       

tta cag ggt att gta tcg tgg ggt tca ggg tgc gca caa aag aac aaa        912
Leu Gln Gly Ile Val Ser Trp Gly Ser Gly Cys Ala Gln Lys Asn Lys           
    290                 295                 300                           

cca ggt gtt tat acc aag gtt tgc aat tat gtt tca tgg atc aag caa        960
Pro Gly Val Tyr Thr Lys Val Cys Asn Tyr Val Ser Trp Ile Lys Gln           
305                 310                 315                 320           

aca att gcg tct aac taa                                                978
Thr Ile Ala Ser Asn                                                       
                325                                                       


<210>  2
<211>  325
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide


<220>
<221>  PEPTIDE
<222>  (1)..(325)

<400>  2

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Asp Asp Asp Asp Lys Ile Val Gly Gly Tyr Thr Cys Gly Ala Asn 
            100                 105                 110         


Thr Val Pro Tyr Gln Val Ser Leu Asn Ser Gly Tyr His Phe Cys Gly 
        115                 120                 125             


Gly Ser Leu Ile Asn Ser Gln Trp Val Val Ser Ala Ala His Cys Tyr 
    130                 135                 140                 


Lys Ser Gly Ile Gln Val Arg Leu Gly Glu Asp Asn Ile Asn Val Val 
145                 150                 155                 160 


Glu Gly Asn Glu Gln Phe Ile Ser Ala Ser Lys Ser Ile Val His Pro 
                165                 170                 175     


Ser Tyr Asn Ser Asn Thr Leu Asn Asn Asp Ile Met Leu Ile Lys Leu 
            180                 185                 190         


Lys Ser Ala Ala Ser Leu Asn Ser Arg Val Ala Ser Ile Ser Leu Pro 
        195                 200                 205             


Thr Ser Cys Ala Ser Ala Gly Thr Gln Cys Leu Ile Ser Gly Trp Gly 
    210                 215                 220                 


Asn Thr Lys Ser Ser Gly Thr Ser Tyr Pro Asp Val Leu Lys Cys Leu 
225                 230                 235                 240 


Lys Ala Pro Ile Leu Ser Asp Ser Ser Cys Lys Ser Ala Tyr Pro Gly 
                245                 250                 255     


Gln Ile Thr Ser Asn Met Phe Cys Ala Gly Tyr Leu Glu Gly Gly Lys 
            260                 265                 270         


Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro Val Val Cys Ser Gly Lys 
        275                 280                 285             


Leu Gln Gly Ile Val Ser Trp Gly Ser Gly Cys Ala Gln Lys Asn Lys 
    290                 295                 300                 


Pro Gly Val Tyr Thr Lys Val Cys Asn Tyr Val Ser Trp Ile Lys Gln 
305                 310                 315                 320 


Thr Ile Ala Ser Asn 
                325 


<210>  3
<211>  975
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exon


<220>
<221>  exon
<222>  (1)..(975)

<400>  3
gtt cca gtt tct ggt aag tct ggt tcc tcc aac act gct gtt tct gct         48
Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala           
1               5                   10                  15                

tcc gat aac gct gct ttg cca cca ttg atc tct tct aga tgt gct cca         96
Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro           
            20                  25                  30                    

cca tct aac aag ggt tcc aag tcc gat ttg cag gct gaa cca tac aac        144
Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn           
        35                  40                  45                        

atg cag aaa aac act gag tgg tac gaa tct cac ggt ggt aac ttg act        192
Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr           
    50                  55                  60                            

tcc atc gga aag aga gat gac aac ttg gtt ggt gga atg act ttg gac        240
Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp           
65                  70                  75                  80            

ttg cca tct gac gct cca cca att tct ttg tcc tct tcc act aac tct        288
Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser           
                85                  90                  95                

gct gat gac gac gac aag atc gtt ggt ggt tac act tgt ggt gct aac        336
Ala Asp Asp Asp Asp Lys Ile Val Gly Gly Tyr Thr Cys Gly Ala Asn           
            100                 105                 110                   

act gtt cca tac cag gtt tca ttg aac tcc gga tac cac ttt tgt ggt        384
Thr Val Pro Tyr Gln Val Ser Leu Asn Ser Gly Tyr His Phe Cys Gly           
        115                 120                 125                       

ggt tcc ttg att aac tcc cag tgg gtt gtc tct gct gct cac tgt tac        432
Gly Ser Leu Ile Asn Ser Gln Trp Val Val Ser Ala Ala His Cys Tyr           
    130                 135                 140                           

aag tcc ggt atc caa gtt aga ttg gga gag gac aac atc aac gtt gtt        480
Lys Ser Gly Ile Gln Val Arg Leu Gly Glu Asp Asn Ile Asn Val Val           
145                 150                 155                 160           

gag ggt aac gag caa ttc att tcc gct tcc aag tcc att gtt cac cca        528
Glu Gly Asn Glu Gln Phe Ile Ser Ala Ser Lys Ser Ile Val His Pro           
                165                 170                 175               

tcc tac aac tcc aac act ttg aac aac gac atc atg ttg atc aag ttg        576
Ser Tyr Asn Ser Asn Thr Leu Asn Asn Asp Ile Met Leu Ile Lys Leu           
            180                 185                 190                   

aag tcc gct gct tct ttg aac tct aga gtt gct tcc atc tcc ttg cca        624
Lys Ser Ala Ala Ser Leu Asn Ser Arg Val Ala Ser Ile Ser Leu Pro           
        195                 200                 205                       

act tca tgt gct tcc gct ggt act caa tgt ttg atc tcc gga tgg ggt        672
Thr Ser Cys Ala Ser Ala Gly Thr Gln Cys Leu Ile Ser Gly Trp Gly           
    210                 215                 220                           

aac act aag tcc tct ggt act tcc tac cca gac gtt ttg aag tgt ttg        720
Asn Thr Lys Ser Ser Gly Thr Ser Tyr Pro Asp Val Leu Lys Cys Leu           
225                 230                 235                 240           

aag gct cca att ttg tct gac tcc tcc tgt aag tct gct tac cca gga        768
Lys Ala Pro Ile Leu Ser Asp Ser Ser Cys Lys Ser Ala Tyr Pro Gly           
                245                 250                 255               

caa atc act tcc aac atg ttc tgt gct ggt tac ttg gaa ggt gga aag        816
Gln Ile Thr Ser Asn Met Phe Cys Ala Gly Tyr Leu Glu Gly Gly Lys           
            260                 265                 270                   

gac tct tgt cag gga gat tct ggt ggt cca gtt gtt tgt tcc ggt aag        864
Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro Val Val Cys Ser Gly Lys           
        275                 280                 285                       

ttg cag ggt att gtt tct tgg ggt tcc ggt tgt gct caa aag aac aag        912
Leu Gln Gly Ile Val Ser Trp Gly Ser Gly Cys Ala Gln Lys Asn Lys           
    290                 295                 300                           

cct ggt gtt tac act aaa gtt tgt aac tac gtt tcc tgg atc aag caa        960
Pro Gly Val Tyr Thr Lys Val Cys Asn Tyr Val Ser Trp Ile Lys Gln           
305                 310                 315                 320           

act atc gct tct aac                                                    975
Thr Ile Ala Ser Asn                                                       
                325                                                       


