                                SEQUENCE LISTING

<110> Crawford, Nigel
      Wang, Rongchen
      Guan, Peizhu
      Chen, Mingsheng
      The Regents of the University of California
      

<120> Nitrate-Responsive Promoter
  

<130> 19452A-004610PC

<140> WO Not yet assigned 
<141> Not yet assigned  

<150> US 61/239,721       
<151> 2009-09-03  

<160> 22

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 192
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic nitrate-responsive enhancer, nitrate-regulated promoter
      (NRP), nitrate inducible promoter Nia1_up_180bp from Arabidopsis
      nitrate reductase (NIA1) distal promoter region containing
      restriction endonuclease sites, NIA1 enhancer fragment

<400> 1
aagcttcgaa aattcaaaca agtgacaaaa ataatattac gacccgtccc tttgtttggt 60
tgccccgttc gtgtatttgc ttggccgttg gtgttcccat gcgccactta gcctccaaag 120
tcttctctct aaactccttt tttataccat aatctctgtt tagtttatac ttaattgcgc 180
tttatcctgc ag                                                     192

<210> 2
<211> 137
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic nitrate-responsive enhancer, nitrate-regulated promoter
      (NRP), nitrate inducible promoter NiR_up_130bp from Arabidopsis
      nitrite reductase (NiR) promoter region containing restriction
      endonuclease site, NiR 3' fragment R

<400> 2
taaacacaat ttaaatagtt tcaaataaat ttagaaagaa taaaacaaat agaaatcaga 60
aggtgtctgt ttcctcctcg caacatacga tcaaagagaa acaacttgac cctttacatt 120
gctcaagagc ttctaga                                                137

<210> 3
<211> 55
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic Cauliflower mosaic virus (CaMV) 35S
      minimal promoter fragment

<400> 3
gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac gctga      55

<210> 4
<211> 91
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic cloning sites and Cauliflower mosaic
      virus (CaMV) 35S minimal promoter fragment

<400> 4
ggatccggta ccgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac 60
acgctgagat ccccgggtag gtcagtccct t                                91

<210> 5
<211> 8
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic cis-regulatory element HVH21 nitrate
      enhancer element (NEE) transcription factor
      binding site

<400> 5
caagtgac                                                          8

<210> 6
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic sequence comprising cis-regulatory
      element HVH21 nitrate enhancer element (NEE)
      transcription factor binding site

<400> 6
tcaaacaagt gacaaaaata atat                                        24

<210> 7
<211> 7
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic cis-regulatory element Myb-2 nitrate
      enhancer element (NEE) transcription factor
      binding site

<400> 7
tggccgt                                                           7

<210> 8
<211> 7
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic cis-regulatory element Alfin1 nitrate
      enhancer element (NEE) transcription factor
      binding site

<400> 8
atgcgcc                                                           7

<210> 9
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic cis-regulatory elements Myb2 + Alfin1
      nitrate enhancer elements (NEE) transcription
      factor binding sites

<400> 9
tgtatttgct tggccgttgg tgttcccatg cgccactta                        39

<210> 10
<211> 109
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic nitrate-responsive enhancer, nitrate-regulated
      promoter (NRP), nitrate inducible promoter NIA1-14e 109 bp
      fragment (3d3) from 5' end of Arabidopsis nitrate reductase
      (NIA1) distal promoter region

<400> 10
tcgaaaattc aaacaagtga caaaaataat attacgaccc gtccctttgt ttggttgccc 60
cgttcgtgta tttgcttggc cgttggtgtt cccatgcgcc acttagcct             109

<210> 11
<211> 180
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic nitrate-responsive enhancer, nitrate-regulated
      promoter (NRP), nitrate inducible promoter Nia1_up_180bp from
      Arabidopsis nitrate reductase (NIA1) distal promoter region
      without restriction endonuclease sites, NIA1 enhancer fragment

<400> 11
cgaaaattca aacaagtgac aaaaataata ttacgacccg tccctttgtt tggttgcccc 60
gttcgtgtat ttgcttggcc gttggtgttc ccatgcgcca cttagcctcc aaagtcttct 120
ctctaaactc cttttttata ccataatctc tgtttagttt atacttaatt gcgctttatc 180


<210> 12
<211> 131
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic nitrate-responsive enhancer, nitrate-regulated
      promoter (NRP), nitrate inducible promoter NiR_up_130bp from
      Arabidopsis nitrite reductase (NiR) promoter region without
      restriction endonuclease site, NiR 3' fragment R

<400> 12
taaacacaat ttaaatagtt tcaaataaat ttagaaagaa taaaacaaat agaaatcaga 60
aggtgtctgt ttcctcctcg caacatacga tcaaagagaa acaacttgac cctttacatt 120
gctcaagagc t                                                      131

<210> 13
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic sequence comprising cis-regulatory
      element HVH21 nitrate enhancer element (NEE)
      transcription factor binding site

<400> 13
ttcaaacaag tgacaaaaat aatat                                       25

<210> 14
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic cis-regulatory elements Myb-2 + Alfin1
      nitrate enhancer elements (NEE) transcription
      factor binding sites

<400> 14
tatttgcttg gccgttggtg ttcccatgcg ccacttagcc t                     41

<210> 15
<211> 317
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic nitrate-responsive promoter (NRP) fusion of Arabidopsis
      nitrate reductase (NIA1) distal promoter region, nitrite reductase
      (NiR) promoter region and Cauliflower mosaic virus (CaMV) 35S
      minimal promoter fragments

<400> 15
cgaaaattca aacaagtgac aaaaataata ttacgacccg tccctttgtt tggttgcccc 60
gttcgtgtat ttgcttggcc gttggtgttc ccatgcgcca cttagcctcc aaagtcttct 120
ctctaaactc cttttttata ccataatctc tgtttagttt atacttaatt gcgctttatc 180
ctgcagtaaa cacaatttaa atagtttcaa ataaatttag aaagaataaa acaaatagaa 240
atcagaaggt gtctgtttcc tcctcgcaac atacgatcaa agagaaacaa cttgaccctt 300
tacattgctc aagagct                                                317

<210> 16
<211> 63
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic wild-type Arabidopsis nitrate
      transporter NRT1.1 (CHL1) partial CDS

<400> 16
aagggtaaac aaaagctgcc acacactgaa caattccgtt cattagataa ggcagcaata 60
agg                                                               63

<210> 17
<211> 21
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic partial wild-type Arabidopsis nitrate
      transporter NRT1.1 (CHL1)

<400> 17
Lys Gly Lys Gln Lys Leu Pro His Thr Glu Gln Phe Arg Ser Leu Asp
 1               5                  10                  15      
Lys Ala Ala Ile Arg
            20      


<210> 18
<211> 63
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic Mut21 (nrg1) Arabidopsis nitrate
      transporter NRT1.1 (CHL1) partial CDS

<400> 18
aagggtaaac aaaagctgcc acacactgaa taattccgtt cattagataa ggcagcaata 60
agg                                                               63

<210> 19
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic partial Mut21 (nrg1) Arabidopsis nitrate
      transporter NRT1.1 (CHL1)

<400> 19
Lys Gly Lys Gln Lys Leu Pro His Thr Glu
 1               5                  10  


<210> 20
<211> 8
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic cis-regulatory element Myb-2 nitrate
      enhancer element (NEE) transcription factor
      binding site

<400> 20
ggccgttg                                                          8

<210> 21
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic cis-regulatory element Alfin1 nitrate
      enhancer element (NEE) transcription factor
      binding site

<400> 21
cgccacttag                                                        10

<210> 22
<211> 412
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic nitrate-responsive promoter (NRP) fusion of Arabidopsis
      nitrate reductase (NIA1) distal promoter region, nitrite reductase
      (NiR) promoter region and Cauliflower mosaic virus (CaMV) 35S
      minimal promoter fragments complete functional promoter

<400> 22
cgaaaattca aacaagtgac aaaaataata ttacgacccg tccctttgtt tggttgcccc 60
gttcgtgtat ttgcttggcc gttggtgttc ccatgcgcca cttagcctcc aaagtcttct 120
ctctaaactc cttttttata ccataatctc tgtttagttt atacttaatt gcgctttatc 180
ctgcagtaaa cacaatttaa atagtttcaa ataaatttag aaagaataaa acaaatagaa 240
atcagaaggt gtctgtttcc tcctcgcaac atacgatcaa agagaaacaa cttgaccctt 300
tacattgctc aagagcttct agaggatccg gtaccgcaag acccttcctc tatataagga 360
agttcatttc atttggagag gacacgctga gatccccggg taggtcagtc cc         412


